Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F202 I0 R1	1397	275.7	9089454	95.2%	8653160	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,846,721	G→T	32.4%	D37Y (GAT→TAT)	hypE →	carbamoyl dehydratase, hydrogenases 1,2,3 maturation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,846,721	0	G	T	32.4%	552.2 / 261.5	307	D37Y (GAT→TAT)	hypE	carbamoyl dehydratase, hydrogenases 1,2,3 maturation protein
Reads supporting (aligned to +/- strand): ref base G (108/99); new base T (48/52); total (156/151)
Fisher's exact test for biased strand distribution p-value = 5.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

TGCGCCGCGTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGACGTTGCGGTC  >  CP009273/2846572‑2846870

tGCGCCGCGTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAg                                                                                                                                                       >  1:1621137/1‑150 (MQ=255)

   gccgcGTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGAtt                                                                                                                                                    >  1:502842/1‑149 (MQ=255)

   gccgcGTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATc                                                                                                                                                    <  2:3109222/150‑1 (MQ=255)

     cgcgTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAg                                                                                                                                                  >  2:2230246/1‑150 (MQ=255)

       cgTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGc                                                                                                                                                <  1:3887149/150‑1 (MQ=255)

            tATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTACTCAACAGCCTGTTTAAGGAAGCCTTTGCCAACCCGAGGCTGGCAGAGCAGGAATATCAGGCACGTc                                                                                                                                           >  2:2671244/1‑150 (MQ=255)

              tCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCtt                                                                                                                                         >  2:747342/1‑150 (MQ=255)

                aGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCAAGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGa                                                                                                                                       >  1:4466281/1‑150 (MQ=255)

                 gTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGAt                                                                                                                                      >  2:4374182/1‑150 (MQ=255)

                 gTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGAt                                                                                                                                      <  1:2485369/150‑1 (MQ=255)

                        cagcagGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGgcgc                                                                                                                               >  1:1101599/1‑150 (MQ=255)

                        cagcagGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGgcgc                                                                                                                               >  1:4473887/1‑150 (MQ=255)

                          gcagGAGAGTGAAGCGTGAATCATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAg                                                                                                                             <  2:275166/150‑1 (MQ=255)

                           cagGAGAGTGAAGCGTGAATAATATCCAACTCGCCCNCGGTAGCGGCGGCCAGGCGATGNAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCNTGATNTGGCGCAGc                                                                                                                            >  1:2705062/1‑150 (MQ=255)

                           cagGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGc                                                                                                                            >  1:34582/1‑150 (MQ=255)

                            agGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCt                                                                                                                           <  1:3525197/150‑1 (MQ=255)

                              gagaGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTgg                                                                                                                         >  2:76729/1‑150 (MQ=255)

                                gagTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTa                                                                                                                       <  2:2037649/150‑1 (MQ=255)

                                 agTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAg                                                                                                                      <  2:3593790/150‑1 (MQ=255)

                                       gCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAg                                                                                                                <  1:4530132/150‑1 (MQ=255)

                                       gCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAg                                                                                                                <  1:3571040/150‑1 (MQ=255)

                                         gTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGc                                                                                                              <  1:238100/150‑1 (MQ=255)

                                         gTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGc                                                                                                              >  1:715181/1‑150 (MQ=255)

                                          tcaataatATCCAACTCGCCCACGGAAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTGTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCg                                                                                                             >  1:4158893/3‑150 (MQ=255)

                                            aataatATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGAc                                                                                                           <  1:3810210/150‑1 (MQ=255)

                                            aataatATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGAc                                                                                                           <  2:2151292/150‑1 (MQ=255)

                                                 tatCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCt                                                                                                      <  1:2762810/150‑1 (MQ=255)

                                                  atCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTg                                                                                                     >  1:1721168/1‑150 (MQ=255)

                                                  atCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTg                                                                                                     >  1:569449/1‑150 (MQ=255)

                                                  atCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTg                                                                                                     >  1:2856297/1‑150 (MQ=255)

                                                   tCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTgg                                                                                                    <  2:1347063/150‑1 (MQ=255)

                                                     cAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCg                                                                                                  <  2:2838724/150‑1 (MQ=255)

                                                       aCTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGtt                                                                                                <  2:1830648/150‑1 (MQ=255)

                                                         tCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTtct                                                                                              >  1:2547293/1‑150 (MQ=255)

                                                         tCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTtct                                                                                              <  2:842154/150‑1 (MQ=255)

                                                           gCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTcc                                                                                            <  1:871833/150‑1 (MQ=255)

                                                            cccACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCa                                                                                           >  2:3581090/1‑150 (MQ=255)

                                                               aCGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCg                                                                                        >  1:2466949/1‑150 (MQ=255)

                                                                 ggTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGAc                                                                                      >  2:1716051/1‑150 (MQ=255)

                                                                    aGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGt                                                                                   >  1:1551405/1‑150 (MQ=255)

                                                                      cggcggCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTa                                                                                 <  1:3187825/150‑1 (MQ=255)

                                                                            ccAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTAt                                                                           >  2:467957/1‑150 (MQ=255)

                                                                             cAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTAtt                                                                          <  2:857799/150‑1 (MQ=255)

                                                                              aGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTg                                                                         >  2:1972638/1‑150 (MQ=255)

                                                                              aGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTg                                                                         >  2:2970892/1‑150 (MQ=255)

                                                                                  gATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGAccc                                                                     <  1:2353878/150‑1 (MQ=255)

                                                                                   aTGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCg                                                                    >  2:1448347/1‑150 (MQ=255)

                                                                                   aTGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCg                                                                    >  2:1491552/1‑150 (MQ=255)

                                                                                   aTGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCg                                                                    <  1:4091884/150‑1 (MQ=255)

                                                                                   aTGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCg                                                                    <  2:224442/150‑1 (MQ=255)

                                                                                   aTGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCg                                                                    <  1:3955191/150‑1 (MQ=255)

                                                                                      cagcaATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTg                                                                 <  2:83778/150‑1 (MQ=255)

                                                                                      cagcaATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTg                                                                 <  1:3811264/150‑1 (MQ=255)

                                                                                      cagcaAGTAATCAACAGCCTGTTTATGGAAGCCTGTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGGAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTg                                                                 <  2:2806678/150‑1 (MQ=255)

                                                                                       agcaATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGt                                                                >  2:3638824/1‑150 (MQ=255)

                                                                                       agcaATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAAACCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGTCTCAGCTAGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGt                                                                >  1:4439570/1‑150 (MQ=255)

                                                                                           aTTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGttctt                                                            >  2:239541/1‑150 (MQ=255)

                                                                                           aTTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGttctt                                                            >  2:1546749/1‑150 (MQ=255)

                                                                                           aTTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTAAGTTATTGACCCGCTGttctt                                                            <  2:2507157/150‑1 (MQ=255)

                                                                                             tAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTcc                                                          <  2:1881159/150‑1 (MQ=255)

                                                                                               aTCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTAAGTTATTGACCCGCTGTTCTTCCCt                                                        <  1:1515864/150‑1 (MQ=255)

                                                                                                  aaCAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGc                                                     <  2:896899/150‑1 (MQ=255)

                                                                                                   aCAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCg                                                    >  2:2184492/1‑150 (MQ=255)

                                                                                                     aGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGt                                                  <  1:3634803/150‑1 (MQ=255)

                                                                                                     aGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGt                                                  >  1:4228912/1‑150 (MQ=255)

                                                                                                      gCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTa                                                 >  2:1628529/1‑150 (MQ=255)

                                                                                                       ccTGTTTATTGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGTCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTaa                                                <  2:2635856/150‑1 (MQ=255)

                                                                                                         tGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCATTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAata                                              <  2:4447838/150‑1 (MQ=255)

                                                                                                          gTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAatat                                             <  1:2230246/150‑1 (MQ=255)

                                                                                                          gTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAatat                                             >  1:1880991/1‑150 (MQ=255)

                                                                                                          gTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAatat                                             >  1:3488659/1‑150 (MQ=255)

                                                                                                           tttATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATc                                            >  2:2819578/1‑150 (MQ=255)

                                                                                                            ttATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCg                                           <  2:330858/150‑1 (MQ=255)

                                                                                                              aTGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGc                                         >  1:763354/1‑150 (MQ=255)

                                                                                                               tGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCa                                        >  1:949885/1‑150 (MQ=255)

                                                                                                               tGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCa                                        >  1:948696/1‑150 (MQ=255)

                                                                                                                ggAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCaa                                       >  2:2879319/1‑150 (MQ=255)

                                                                                                                      cTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGc                                 >  1:3750047/1‑150 (MQ=255)

                                                                                                                       tttGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCg                                >  2:4315971/1‑150 (MQ=255)

                                                                                                                          gCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGAtt                             <  1:3246859/150‑1 (MQ=255)

                                                                                                                            cAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTg                           <  1:1572183/150‑1 (MQ=255)

                                                                                                                            cAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTg                           >  2:321380/1‑150 (MQ=255)

                                                                                                                             aaCCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGc                          >  2:1023081/1‑150 (MQ=255)

                                                                                                                                    ggctggcAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAg                   >  1:14546/1‑150 (MQ=255)

                                                                                                                                       tggcAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCa                <  2:502092/150‑1 (MQ=255)

                                                                                                                                        ggcAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCaa               <  1:406750/150‑1 (MQ=255)

                                                                                                                                        ggcAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCaa               >  1:1674970/1‑150 (MQ=255)

                                                                                                                                        ggcAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCaa               >  1:4518707/1‑150 (MQ=255)

                                                                                                                                        ggcAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTAAGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCaa               >  2:4433764/1‑150 (MQ=255)

                                                                                                                                         gcAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAAt              <  1:2080414/150‑1 (MQ=255)

                                                                                                                                          cAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATg             >  2:4455982/1‑150 (MQ=255)

                                                                                                                                           agagCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGAGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTGACTGTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGa            >  1:2932250/1‑150 (MQ=255)

                                                                                                                                            gagCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGAc           >  2:4262028/1‑150 (MQ=255)

                                                                                                                                            gagCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGAc           >  2:1787788/1‑150 (MQ=255)

                                                                                                                                             agCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGACg          >  1:103405/1‑150 (MQ=255)

                                                                                                                                               catgAATATCATGCACGTCTTGATCTGGCGCAGCTGGTATCTGTAGGAGTCGGTATGTCTTTCTCGACCTACAGTTTCGTTATTGACCCGCTTTTCTTTCCTGGCGGTAATATGGGCAAGCTGGCGTTTTGCTGCACAGACTATGACGtt        <  2:2193417/147‑1 (MQ=255)

                                                                                                                                               cAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGACGtt        <  1:3735161/150‑1 (MQ=255)

                                                                                                                                               cAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGACGtt        >  2:2546804/1‑150 (MQ=255)

                                                                                                                                                aGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGACGTTg       >  2:3830059/1‑150 (MQ=255)

                                                                                                                                                 ggAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGACGTTGc      <  1:3018963/150‑1 (MQ=255)

TGCGCCGCGTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGACGTTGCGGTC  >  CP009273/2846572‑2846870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A