Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,846,721 G→T 6.0% D37Y (GAT→TAT)  hypE → carbamoyl dehydratase, hydrogenases 1,2,3 maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,846,7210GT 6.0% 1083.7 / 28.5 281D37Y (GAT→TAT) hypEcarbamoyl dehydratase, hydrogenases 1,2,3 maturation protein
Reads supporting (aligned to +/- strand):  ref base G (137/127);  new base T (10/7);  total (147/134)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 282 total aligned reads displayed.

CGCCGCGTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGACGTTGCGGTC  >  CP009273/2846574‑2846870
                                                                                                                                                   |                                                                                                                                                     
cgccgcGTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGAt                                                                                                                                                     <  1:3653392/150‑1 (MQ=255)
cgccgcGTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGAt                                                                                                                                                     <  1:1670287/150‑1 (MQ=255)
   cgcgTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAg                                                                                                                                                  >  1:3855092/1‑150 (MQ=255)
   cgcgTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAg                                                                                                                                                  <  1:388686/150‑1 (MQ=255)
   cgcgTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAg                                                                                                                                                  <  2:3944516/150‑1 (MQ=255)
     cgTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGc                                                                                                                                                <  1:4239124/150‑1 (MQ=255)
        ggTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACg                                                                                                                                             <  2:3065010/150‑1 (MQ=255)
              aGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGa                                                                                                                                       >  2:1025882/1‑150 (MQ=255)
                 aTCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCt                                                                                                                                    <  2:2404866/150‑1 (MQ=255)
                  tCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTg                                                                                                                                   >  1:3938486/1‑150 (MQ=255)
                   cGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTgg                                                                                                                                  >  1:868903/1‑150 (MQ=255)
                     tCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCATTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTAGATCTGgcg                                                                                                                                >  1:1464853/1‑150 (MQ=255)
                         cagGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGc                                                                                                                            >  1:1167009/1‑150 (MQ=255)
                          agGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCt                                                                                                                           >  1:2561001/1‑150 (MQ=255)
                             agagTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGt                                                                                                                        <  1:1785587/150‑1 (MQ=255)
                             agagTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGt                                                                                                                        <  1:3406248/150‑1 (MQ=255)
                               agTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAg                                                                                                                      <  1:8605/150‑1 (MQ=255)
                                gTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGc                                                                                                                     >  2:2973776/1‑150 (MQ=255)
                                       gTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGc                                                                                                              <  1:1065958/150‑1 (MQ=255)
                                        tGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCg                                                                                                             <  1:1651881/150‑1 (MQ=255)
                                        tGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCg                                                                                                             <  2:2780428/150‑1 (MQ=255)
                                           ataataTCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGAcc                                                                                                          <  1:3488934/150‑1 (MQ=255)
                                           ataataTCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGAcc                                                                                                          <  2:3943692/150‑1 (MQ=255)
                                             aataTCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATAAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGt                                                                                                        >  1:559391/1‑150 (MQ=255)
                                              atatCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTc                                                                                                       >  1:2858947/1‑150 (MQ=255)
                                               tatCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCt                                                                                                      >  2:444478/1‑150 (MQ=255)
                                                    aaCTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGt                                                                                                 >  1:3632015/1‑150 (MQ=255)
                                                     aCTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGtt                                                                                                >  2:3103321/1‑150 (MQ=255)
                                                     aCTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGtt                                                                                                >  2:656795/1‑150 (MQ=255)
                                                     aCTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGtt                                                                                                >  2:2551852/1‑150 (MQ=255)
                                                     aCTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGtt                                                                                                <  2:4020642/150‑1 (MQ=255)
                                                      cTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTtc                                                                                               >  2:1651465/1‑150 (MQ=255)
                                                      cTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTtc                                                                                               <  2:748513/150‑1 (MQ=255)
                                                       tCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTtct                                                                                              <  1:369067/150‑1 (MQ=255)
                                                           ccACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCAc                                                                                          >  1:1893304/1‑150 (MQ=255)
                                                               ggTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGAc                                                                                      >  2:2844494/1‑150 (MQ=255)
                                                               ggTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGAc                                                                                      >  2:2155727/1‑150 (MQ=255)
                                                               ggTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGAc                                                                                      >  1:652378/1‑150 (MQ=255)
                                                               ggTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGAAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGAc                                                                                      >  1:652587/1‑150 (MQ=255)
                                                                gTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACa                                                                                     >  1:3738958/1‑150 (MQ=255)
                                                                gTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACa                                                                                     >  2:3471949/1‑150 (MQ=255)
                                                                     ggcggcCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTAc                                                                                >  1:1372741/1‑150 (MQ=255)
                                                                      gcggcCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACg                                                                               <  1:2325500/150‑1 (MQ=255)
                                                                           cAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTAtt                                                                          >  1:3584034/1‑150 (MQ=255)
                                                                                gATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGAccc                                                                     <  2:1993180/150‑1 (MQ=255)
                                                                                     agcaATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGt                                                                <  1:4010725/150‑1 (MQ=255)
                                                                                     agcaATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGt                                                                <  2:3428756/150‑1 (MQ=255)
                                                                                     agcaATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGt                                                                <  2:4511132/150‑1 (MQ=255)
                                                                                     agcaATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGt                                                                >  1:2757187/1‑150 (MQ=255)
                                                                                      gcaATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGtt                                                               >  2:3302473/1‑150 (MQ=255)
                                                                                      gcaATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGtt                                                               <  2:4626949/150‑1 (MQ=255)
                                                                                      gcaATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGtt                                                               <  1:3436715/150‑1 (MQ=255)
                                                                                       caATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGttc                                                              >  2:4417843/1‑150 (MQ=255)
                                                                                           tAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTGCTCCACCGACAGTTACGTTAGTGACCCGCTGTTCTTcc                                                          >  2:2948772/1‑150 (MQ=255)
                                                                                            aaTCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTccc                                                         >  1:2985167/1‑150 (MQ=255)
                                                                                             aTCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCt                                                        >  1:1989818/1‑150 (MQ=255)
                                                                                             aTCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCt                                                        >  2:3376112/1‑150 (MQ=255)
                                                                                              tCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTg                                                       <  1:3303504/150‑1 (MQ=255)
                                                                                                 aCAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCg                                                    <  1:3074924/150‑1 (MQ=255)
                                                                                                  cAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCgg                                                   <  2:1353852/150‑1 (MQ=255)
                                                                                                  cAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCgg                                                   >  2:2095883/1‑150 (MQ=255)
                                                                                                   aGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGt                                                  >  2:3867796/1‑150 (MQ=255)
                                                                                                   aGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGt                                                  >  2:1784820/1‑150 (MQ=255)
                                                                                                   aGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGt                                                  <  2:1178641/150‑1 (MQ=255)
                                                                                                     ccTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTaa                                                <  1:1280065/150‑1 (MQ=255)
                                                                                                     ccTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTaa                                                <  1:3372593/150‑1 (MQ=255)
                                                                                                     ccTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTaa                                                <  2:3342171/150‑1 (MQ=255)
                                                                                                     ccTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTaa                                                <  1:1508301/150‑1 (MQ=255)
                                                                                                        gTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAatat                                             >  1:4330554/1‑150 (MQ=255)
                                                                                                           tATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCgg                                          >  1:2229992/1‑150 (MQ=255)
                                                                                                            aTGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGc                                         <  2:1138875/150‑1 (MQ=255)
                                                                                                             tGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCa                                        <  2:2854969/150‑1 (MQ=255)
                                                                                                                aaGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCTGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGc                                     >  1:3162712/1‑150 (MQ=255)
                                                                                                                  gCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTg                                   >  1:1843620/1‑150 (MQ=255)
                                                                                                                     tttGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCg                                >  2:2568135/1‑150 (MQ=255)
                                                                                                                       tGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGAt                              >  1:1340834/1‑150 (MQ=255)
                                                                                                                       tGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGAt                              >  2:1680231/1‑150 (MQ=255)
                                                                                                                        gCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTACACCGACAGTTACGTTTTTGACCCGCTGTTATTCCCTGGCGGTAATATCGGCAAGCTGGCGAtt                             <  2:1298005/150‑1 (MQ=255)
                                                                                                                         ccAACCCGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGAttt                            <  1:2106134/150‑1 (MQ=255)
                                                                                                                         ccAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGAttt                            <  2:1720976/150‑1 (MQ=255)
                                                                                                                           aaCCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGc                          <  1:1381416/150‑1 (MQ=255)
                                                                                                                           aaCCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGc                          <  1:3571509/150‑1 (MQ=255)
                                                                                                                            aCCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCg                         <  2:3718478/150‑1 (MQ=255)
                                                                                                                             cccGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCCAGCTGGCGATTTGCgg                        >  1:4105655/1‑150 (MQ=255)
                                                                                                                               cGTGGCTGGCAGAGCAGGAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGca                      >  1:2181505/1‑150 (MQ=255)
                                                                                                                               cGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGca                      >  1:2460740/1‑150 (MQ=255)
                                                                                                                                gTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTtc                                                                                               >  2:1476055/1‑76 (MQ=255)
                                                                                                                                gTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGcac                     >  2:3788148/1‑150 (MQ=255)
                                                                                                                                    ctggcAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGcc                 >  2:2495045/1‑150 (MQ=255)
                                                                                                                                     tggcAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCa                <  2:1589087/150‑1 (MQ=255)
                                                                                                                                      ggcAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCaa               <  1:1230954/150‑1 (MQ=255)
                                                                                                                                        cAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATg             >  1:1409583/1‑150 (MQ=255)
                                                                                                                                         agagCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGa            >  1:3193825/1‑150 (MQ=255)
                                                                                                                                         agagCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGa            <  2:56098/150‑1 (MQ=255)
                                                                                                                                          gagCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGAc           >  2:3059766/1‑150 (MQ=255)
                                                                                                                                          gagCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCCCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGAc           <  1:259236/150‑1 (MQ=255)
                                                                                                                                               ggAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGACGTTGc      <  1:124605/150‑1 (MQ=255)
                                                                                                                                                gAATATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGACGTTGCg     >  1:1838562/1‑150 (MQ=255)
                                                                                                                                                  aGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGACGTTGCGGt   >  2:4519361/1‑150 (MQ=255)
                                                                                                                                                   gATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGACGTTGCGGTc  <  2:876844/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGCCGCGTGGTATCAGTATCGTCAGCAGGAGAGTGAAGCGTGAATAATATCCAACTCGCCCACGGTAGCGGCGGCCAGGCGATGCAGCAATTAATCAACAGCCTGTTTATGGAAGCCTTTGCCAACCCGTGGCTGGCAGAGCAGGAAGATCAGGCACGTCTTGATCTGGCGCAGCTGGTAGCGGAAGGCGACCGTCTGGCGTTCTCCACCGACAGTTACGTTATTGACCCGCTGTTCTTCCCTGGCGGTAATATCGGCAAGCTGGCGATTTGCGGCACAGCCAATGACGTTGCGGTC  >  CP009273/2846574‑2846870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A