Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F202 I0 R1 1397 275.7 9089454 95.2% 8653160 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,208,942 C→A 48.9% A140E (GCG→GAG)  aceK → isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,208,9420CA48.9% 140.4 / inf 313A140E (GCG→GAG) aceKisocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base C (68/92);  new base A (71/82);  total (139/174)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 315 total aligned reads displayed.

GGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCG  >  CP009273/4208796‑4209091
                                                                                                                                                  |                                                                                                                                                     
ggATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGaa                                                                                                                                                    >  2:1343198/1‑150 (MQ=255)
ggATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGaa                                                                                                                                                    >  2:2427454/1‑150 (MQ=255)
ggATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTATAGCTCTCAGCCAGAGCGCCGCTTTCGTACAATTCCCCGCCCGCTGGCGaa                                                                                                                                                    >  2:2950486/1‑150 (MQ=255)
      cccGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACtt                                                                                                                                              <  2:3049442/150‑1 (MQ=255)
       ccGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACttt                                                                                                                                             >  1:4005744/1‑150 (MQ=255)
        cgcgCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTc                                                                                                                                            <  2:1667822/150‑1 (MQ=255)
        cgcgCTTCGAGAGTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTc                                                                                                                                            <  2:3087734/150‑1 (MQ=255)
          cgcTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTATCAc                                                                                                                                          >  2:2744431/1‑150 (MQ=255)
            cTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGGTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCAccc                                                                                                                                        >  2:810009/1‑150 (MQ=255)
             ttCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCCCTTTCGTACCATTACCCGCCCCCTGGCGAAAGACATTCAcccc                                                                                                                                       >  1:2894952/1‑150 (MQ=255)
             gtCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCAcccc                                                                                                                                       <  2:2912212/149‑1 (MQ=255)
              tCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCg                                                                                                                                      <  1:2537844/150‑1 (MQ=255)
                 agaTTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATc                                                                                                                                   >  2:3849157/1‑150 (MQ=255)
                 agaTTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATc                                                                                                                                   <  2:2052328/150‑1 (MQ=255)
                        ggAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCACTCAGCCAGAGCGCCCCTTTCGTAACATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTg                                                                                                                            >  1:1810041/1‑150 (MQ=255)
                         gagaGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTgg                                                                                                                           <  2:1960773/150‑1 (MQ=255)
                         gagaGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTgg                                                                                                                           <  2:1108451/150‑1 (MQ=255)
                         gagaGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTgg                                                                                                                           >  1:4390302/1‑150 (MQ=255)
                               ttttttAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCGGGGAAtat                                                                                                                     >  2:333535/1‑148 (MQ=255)
                                tttttAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAAtctc                                                                                                                    <  1:1878669/150‑1 (MQ=255)
                                tttttAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAAtctc                                                                                                                    <  1:3717755/150‑1 (MQ=255)
                                  tttAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTa                                                                                                                  <  2:1466188/150‑1 (MQ=255)
                                    tAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACt                                                                                                                <  1:2827791/150‑1 (MQ=255)
                                      aCTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTGAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGa                                                                                                              <  2:3108670/150‑1 (MQ=255)
                                       cTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGAt                                                                                                             <  1:1174840/150‑1 (MQ=255)
                                        tCCGTGTACTGTCGGTTATCTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGATAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATg                                                                                                            <  2:2811521/150‑1 (MQ=255)
                                          cGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATgcg                                                                                                          >  1:2605869/1‑150 (MQ=255)
                                              tACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGtt                                                                                                      >  2:2213638/1‑150 (MQ=255)
                                               aCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTa                                                                                                     <  2:1807000/150‑1 (MQ=255)
                                               aCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTa                                                                                                     <  1:4037773/150‑1 (MQ=255)
                                               aCTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTa                                                                                                     <  1:3619689/150‑1 (MQ=255)
                                                cTGTCGGTTATTTGACCACCTCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCGACTGATGCGCGTTAt                                                                                                    >  1:3494347/1‑150 (MQ=255)
                                                cTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTAt                                                                                                    >  1:3494281/1‑150 (MQ=255)
                                                cTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTATCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTAt                                                                                                    >  2:679832/1‑150 (MQ=255)
                                                  gTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCa                                                                                                  <  2:3132858/150‑1 (MQ=255)
                                                    cGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGc                                                                                                >  1:4054393/1‑150 (MQ=255)
                                                      gTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGa                                                                                              <  2:3898952/150‑1 (MQ=255)
                                                       ttATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGAc                                                                                             >  2:4038569/1‑150 (MQ=255)
                                                           ttGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTAc                                                                                         <  2:3156648/150‑1 (MQ=255)
                                                            tGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGACTCTCTACTGATGCGCGTTATCAGCGACCTAcc                                                                                        >  2:4337603/1‑150 (MQ=255)
                                                            tGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTAcc                                                                                        <  2:4210702/150‑1 (MQ=255)
                                                             gACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCg                                                                                       <  2:425853/150‑1 (MQ=255)
                                                              accaccGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGc                                                                                      >  1:743243/1‑150 (MQ=255)
                                                               ccaccGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGct                                                                                     >  2:3749646/1‑150 (MQ=255)
                                                                caccGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGctg                                                                                    >  2:867089/1‑150 (MQ=255)
                                                                    gctcgctTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGctgcgcc                                                                                >  1:592503/1‑150 (MQ=255)
                                                                    gctcgctTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGctgcgcc                                                                                >  1:591727/1‑150 (MQ=255)
                                                                     ctcgctTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGctgcgcct                                                                               >  2:1535428/1‑150 (MQ=255)
                                                                       cgctTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCAGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGctgcgcctgc                                                                             >  1:3306624/1‑150 (MQ=255)
                                                                        gctTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACAGATGCGCGTTATCAGCGACCAACCGcagcgccagcg                                                                            >  2:2168544/1‑150 (MQ=255)
                                                                         ctTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGctgcgcctgcgc                                                                           >  2:4229237/1‑150 (MQ=255)
                                                                         ctTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGctgcgcctgcgc                                                                           >  2:364863/1‑150 (MQ=255)
                                                                              tCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCa                                                                      >  2:2353504/1‑150 (MQ=255)
                                                                              tCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCa                                                                      <  1:4182098/150‑1 (MQ=255)
                                                                                 cGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTCCCGCTGCGCCTGCGCTGGCAGaa                                                                   >  2:649050/1‑150 (MQ=255)
                                                                                    gCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATaa                                                                <  1:3974767/150‑1 (MQ=255)
                                                                                     cGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATaaa                                                               <  2:87214/150‑1 (MQ=255)
                                                                                     cGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATACCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATaaa                                                               >  2:4451618/1‑150 (MQ=255)
                                                                                      ggCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAGTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATaaaa                                                              <  1:3857201/150‑1 (MQ=255)
                                                                                      ggCATTTTATCTTTAGCTCACAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCGGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATaaaa                                                              <  2:4357824/150‑1 (MQ=255)
                                                                                        cattttATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGATAGACTTTCACCCCGATCTCGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGc                                                            <  1:1114810/148‑1 (MQ=255)
                                                                                        cTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGc                                                            <  2:4341875/150‑1 (MQ=255)
                                                                                          ttttATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCg                                                          >  1:72417/1‑150 (MQ=255)
                                                                                          ttttATCTTGAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCg                                                          <  2:1880268/150‑1 (MQ=255)
                                                                                            ttATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTg                                                        <  1:1319723/150‑1 (MQ=255)
                                                                                              aTCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGAc                                                      <  1:1615806/150‑1 (MQ=255)
                                                                                               tCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAGTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACa                                                     <  1:1240135/150‑1 (MQ=255)
                                                                                                cTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACAt                                                    >  1:1901304/1‑150 (MQ=255)
                                                                                                 tttAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATc                                                   <  2:2123931/150‑1 (MQ=255)
                                                                                                  ttAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATcc                                                  >  1:540704/1‑150 (MQ=255)
                                                                                                     gCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCcatt                                               <  2:3423383/150‑1 (MQ=255)
                                                                                                       tctcAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCcattac                                             <  2:540704/150‑1 (MQ=255)
                                                                                                           aGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGACTAAAAGCCGTGACATCcattacatta                                         >  1:2528666/1‑150 (MQ=255)
                                                                                                            gCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACattat                                        <  1:2006010/150‑1 (MQ=255)
                                                                                                            gCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACattat                                        <  1:3397144/150‑1 (MQ=255)
                                                                                                             ccAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACattatt                                       <  1:2269426/150‑1 (MQ=255)
                                                                                                              cAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTc                                      <  2:4126105/150‑1 (MQ=255)
                                                                                                               agagCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCg                                     >  1:4042116/1‑150 (MQ=255)
                                                                                                                gagCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGc                                    <  1:662473/150‑1 (MQ=255)
                                                                                                                       gcTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGa                             <  2:3522151/150‑1 (MQ=255)
                                                                                                                       gcTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGa                             <  2:3522190/150‑1 (MQ=255)
                                                                                                                       gcTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGa                             <  2:4464853/150‑1 (MQ=255)
                                                                                                                       gcTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGa                             <  1:3212133/150‑1 (MQ=255)
                                                                                                                        cTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGAc                            <  1:4348645/150‑1 (MQ=255)
                                                                                                                        cTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGAc                            <  2:2093449/150‑1 (MQ=255)
                                                                                                                            cGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGaa                        <  1:145751/150‑1 (MQ=255)
                                                                                                                             gTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGaaa                       >  1:1006278/1‑150 (MQ=255)
                                                                                                                             gTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGaaa                       >  2:2956482/1‑150 (MQ=255)
                                                                                                                              tACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAAc                      <  2:1708222/150‑1 (MQ=255)
                                                                                                                              tACCAGTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAAc                      <  2:2852140/150‑1 (MQ=255)
                                                                                                                               aCCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACg                     <  1:2976852/150‑1 (MQ=255)
                                                                                                                                 cATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCt                   >  2:41679/1‑150 (MQ=255)
                                                                                                                                    tCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTggg                <  2:1866295/150‑1 (MQ=255)
                                                                                                                                    tCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTggg                >  2:3273293/1‑150 (MQ=255)
                                                                                                                                     ccccGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTgggg               >  1:2383081/1‑150 (MQ=255)
                                                                                                                                     ccccGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTgggg               >  1:2383099/1‑150 (MQ=255)
                                                                                                                                          cccgcTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGgacaga          >  2:1195330/1‑150 (MQ=255)
                                                                                                                                            cgcTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGgacagaca        >  2:3119414/1‑150 (MQ=255)
                                                                                                                                            cgcTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGgacagaca        <  2:2504549/150‑1 (MQ=255)
                                                                                                                                              cTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAAc      <  2:4257443/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAACCTCG  >  CP009273/4208796‑4209091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAA  >  CP009273/4208798‑4209086
                                                                                                                                                |                                                                                                                                                
ATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAG                                                                                                                                             >  SRR19973076.3260564/1‑150 (MQ=60)
ATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATATTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAG                                                                                                                                             >  SRR19973076.246001/1‑150 (MQ=60)
  TACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGAC                                                                                                                                           <  SRR19973076.1784183/150‑1 (MQ=60)
  TACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATATTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGAC                                                                                                                                           >  SRR19973076.2225535/1‑150 (MQ=60)
     CCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTT                                                                                                                                        >  SRR19973076.4011666/1‑150 (MQ=60)
     CCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTT                                                                                                                                        >  SRR19973076.4011667/1‑150 (MQ=60)
      CGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTC                                                                                                                                       >  SRR19973076.2537571/1‑150 (MQ=60)
       GCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCA                                                                                                                                      >  SRR19973076.4011630/1‑150 (MQ=60)
        CGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCAC                                                                                                                                     >  SRR19973076.3142072/1‑150 (MQ=60)
         GCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACC                                                                                                                                    <  SRR19973076.4079869/150‑1 (MQ=60)
           TTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCCCTTTCGTACCATTACCCGCCCCCTGGCGAAAGACATTCACCCC                                                                                                                                  >  SRR19973076.2899153/1‑150 (MQ=60)
            TCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCG                                                                                                                                 <  SRR19973076.2541536/150‑1 (MQ=60)
             CGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGA                                                                                                                                >  SRR19973076.1344969/1‑150 (MQ=60)
             CGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGA                                                                                                                                >  SRR19973076.1346746/1‑150 (MQ=60)
               AGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATC                                                                                                                              <  SRR19973076.3996745/150‑1 (MQ=60)
                 ATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCAC                                                                                                                            <  SRR19973076.3918202/150‑1 (MQ=60)
                     CGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCT                                                                                                                        <  SRR19973076.3591600/150‑1 (MQ=60)
                      GGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCACTCAGCCAGAGCGCCCCTTTCGTAACATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTG                                                                                                                       >  SRR19973076.1812648/1‑150 (MQ=60)
                       GAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGG                                                                                                                      >  SRR19973076.4396779/1‑150 (MQ=60)
                             TTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCT                                                                                                                >  SRR19973076.1868991/1‑150 (MQ=60)
                              TTTTTAACTCCGTGTACTGTCTGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTC                                                                                                               <  SRR19973076.1881409/150‑1 (MQ=60)
                              TTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTC                                                                                                               <  SRR19973076.3723207/150‑1 (MQ=60)
                              TTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTC                                                                                                               <  SRR19973076.3724467/150‑1 (MQ=60)
                              TTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTC                                                                                                               <  SRR19973076.1881372/150‑1 (MQ=60)
                              TTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCACGAGCGGCTTTTTATCTTTAGCTCACATCCAGAGCGCCGCTTTCGAACCAATACCCGCCCGCTGGCTAAAGACGTTCAACCCGATCACGGCTGGGAAaatc                                                                                                               >  SRR19973076.4388216/1‑146 (MQ=60)
                                  TAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACT                                                                                                           <  SRR19973076.2831880/150‑1 (MQ=60)
                                   AACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTG                                                                                                          >  SRR19973076.1869711/1‑150 (MQ=60)
                                    ACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGA                                                                                                         <  SRR19973076.1152342/150‑1 (MQ=60)
                                     CTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGTT                                                                                                        <  SRR19973076.1176554/150‑1 (MQ=60)
                                     CTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGAT                                                                                                        <  SRR19973076.1176582/150‑1 (MQ=60)
                                        CGTGTACTGTCGGTTATTTGACCACCGCTCTCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCG                                                                                                     >  SRR19973076.4467522/1‑150 (MQ=60)
                                        CGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCG                                                                                                     >  SRR19973076.2609664/1‑150 (MQ=60)
                                             ACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTA                                                                                                <  SRR19973076.3624949/150‑1 (MQ=60)
                                             ACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTA                                                                                                <  SRR19973076.4043738/150‑1 (MQ=60)
                                             ACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTA                                                                                                <  SRR19973076.4043752/150‑1 (MQ=60)
                                             ACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTA                                                                                                <  SRR19973076.4043774/150‑1 (MQ=60)
                                              CTGTCGGTTATTTGACCACCTCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCGACTGATGCGCGTTAT                                                                                               >  SRR19973076.3499417/1‑150 (MQ=60)
                                              CTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTAT                                                                                               >  SRR19973076.3499351/1‑150 (MQ=60)
                                              CTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTAT                                                                                               >  SRR19973076.3499406/1‑150 (MQ=60)
                                              CTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTAT                                                                                               >  SRR19973076.3499408/1‑150 (MQ=60)
                                               TGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGggggggcgctctacggatgcgcgttttc                                                                                              >  SRR19973076.2605801/1‑122 (MQ=60)
                                                  CGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGC                                                                                           >  SRR19973076.4060385/1‑150 (MQ=60)
                                                   GGTTATTTGGCCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCG                                                                                          >  SRR19973076.580287/1‑150 (MQ=60)
                                                          TGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATATTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACC                                                                                   >  SRR19973076.2359874/1‑150 (MQ=60)
                                                           GACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCG                                                                                  <  SRR19973076.1685323/150‑1 (MQ=60)
                                                            ACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGC                                                                                 >  SRR19973076.744344/1‑150 (MQ=60)
                                                             CCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCT                                                                                <  SRR19973076.4198692/150‑1 (MQ=60)
                                                                  GCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCC                                                                           >  SRR19973076.592643/1‑150 (MQ=60)
                                                                  GCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCC                                                                           >  SRR19973076.593229/1‑150 (MQ=60)
                                                                  GCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCC                                                                           >  SRR19973076.593390/1‑150 (MQ=60)
                                                                  GCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCC                                                                           >  SRR19973076.593419/1‑150 (MQ=60)
                                                                     CGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGC                                                                        >  SRR19973076.3311810/1‑150 (MQ=60)
                                                                     CGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCAGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGC                                                                        >  SRR19973076.3311424/1‑150 (MQ=60)
                                                                      GCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCG                                                                       <  SRR19973076.401750/150‑1 (MQ=60)
                                                                       CTTACTCCCGAGCGGCTTTTTATCTTTATCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGC                                                                      >  SRR19973076.632568/1‑150 (MQ=60)
                                                                       CTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGC                                                                      >  SRR19973076.1307981/1‑150 (MQ=60)
                                                                        TTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCT                                                                     >  SRR19973076.284963/1‑150 (MQ=60)
                                                                        TTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCT                                                                     >  SRR19973076.285882/1‑150 (MQ=60)
                                                                         TACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTG                                                                    <  SRR19973076.93090/150‑1 (MQ=60)
                                                                            TCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCA                                                                 <  SRR19973076.1182629/150‑1 (MQ=60)
                                                                            TCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCA                                                                 <  SRR19973076.4188280/150‑1 (MQ=60)
                                                                            TCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCA                                                                 <  SRR19973076.4188271/150‑1 (MQ=60)
                                                                             CCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAG                                                                <  SRR19973076.1948147/150‑1 (MQ=60)
                                                                             CCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAG                                                                <  SRR19973076.1948211/150‑1 (MQ=60)
                                                                                GAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAAT                                                             >  SRR19973076.2906996/1‑150 (MQ=60)
                                                                                 AGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATA                                                            >  SRR19973076.2627456/1‑150 (MQ=60)
                                                                                 AGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATA                                                            >  SRR19973076.2370512/1‑150 (MQ=60)
                                                                                  GCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAA                                                           <  SRR19973076.3980618/150‑1 (MQ=60)
                                                                                   CGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAA                                                          <  SRR19973076.658438/150‑1 (MQ=60)
                                                                                    GGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAGTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAA                                                         <  SRR19973076.3862886/150‑1 (MQ=60)
                                                                                    GGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAGTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAA                                                         <  SRR19973076.3863000/150‑1 (MQ=60)
                                                                                     GCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAG                                                        >  SRR19973076.3734820/1‑150 (MQ=60)
                                                                                     GCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAG                                                        >  SRR19973076.3735495/1‑150 (MQ=60)
                                                                                      CATTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGATAGACTTTCACCCCGATCTCGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGC                                                       <  SRR19973076.1116458/150‑1 (MQ=60)
                                                                                      CATTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGC                                                       <  SRR19973076.1903702/150‑1 (MQ=60)
                                                                                        TTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCG                                                     >  SRR19973076.72571/1‑150 (MQ=60)
                                                                                          TTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTG                                                   <  SRR19973076.1321663/150‑1 (MQ=60)
                                                                                           TATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGA                                                  >  SRR19973076.4370799/1‑150 (MQ=60)
                                                                                            ATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGAC                                                 <  SRR19973076.1618173/150‑1 (MQ=60)
                                                                                             TCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACA                                                <  SRR19973076.1098964/150‑1 (MQ=60)
                                                                                             TCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATATAAGCCGTGACA                                                <  SRR19973076.1241953/150‑1 (MQ=60)
                                                                                             TCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACA                                                <  SRR19973076.734999/150‑1 (MQ=60)
                                                                                             TCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACA                                                <  SRR19973076.735082/150‑1 (MQ=60)
                                                                                             TCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCAGTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACA                                                <  SRR19973076.1241969/150‑1 (MQ=60)
                                                                                              CTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACAT                                               >  SRR19973076.1904043/1‑150 (MQ=60)
                                                                                                TTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCC                                             <  SRR19973076.2469911/150‑1 (MQ=60)
                                                                                                TTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCC                                             >  SRR19973076.541579/1‑150 (MQ=60)
                                                                                                TTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCC                                             >  SRR19973076.541561/1‑150 (MQ=60)
                                                                                                  AGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCAT                                           >  SRR19973076.1292522/1‑150 (MQ=60)
                                                                                                   GCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATT                                          <  SRR19973076.269557/150‑1 (MQ=60)
                                                                                                    CTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGTAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTA                                         <  SRR19973076.671749/150‑1 (MQ=60)
                                                                                                    CTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTA                                         <  SRR19973076.1607119/150‑1 (MQ=60)
                                                                                                    CTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTA                                         <  SRR19973076.1607137/150‑1 (MQ=60)
                                                                                                      CTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACA                                       <  SRR19973076.1775538/150‑1 (MQ=60)
                                                                                                      CTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACA                                       <  SRR19973076.2017375/150‑1 (MQ=60)
                                                                                                         AGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGACTAAAAGCCGTGACATCCATTACATTA                                    >  SRR19973076.2532346/1‑150 (MQ=60)
                                                                                                         AGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTA                                    >  SRR19973076.2532347/1‑150 (MQ=60)
                                                                                                          GCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTAT                                   <  SRR19973076.2008941/150‑1 (MQ=60)
                                                                                                          GCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTAT                                   <  SRR19973076.3402066/150‑1 (MQ=60)
                                                                                                           CCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATT                                  <  SRR19973076.2272676/150‑1 (MQ=60)
                                                                                                             AGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCG                                >  SRR19973076.3402932/1‑150 (MQ=60)
                                                                                                             AGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCG                                >  SRR19973076.3402896/1‑150 (MQ=60)
                                                                                                             AGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCG                                >  SRR19973076.4048236/1‑150 (MQ=60)
                                                                                                             AGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCG                                >  SRR19973076.4048143/1‑150 (MQ=60)
                                                                                                             AGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCG                                >  SRR19973076.4048088/1‑150 (MQ=60)
                                                                                                             AGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCG                                <  SRR19973076.67279/150‑1 (MQ=60)
                                                                                                             AGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCG                                <  SRR19973076.67375/150‑1 (MQ=60)
                                                                                                              GAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGC                               <  SRR19973076.4160721/150‑1 (MQ=60)
                                                                                                              GAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGC                               <  SRR19973076.2946309/150‑1 (MQ=60)
                                                                                                              GAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGC                               <  SRR19973076.663467/150‑1 (MQ=60)
                                                                                                              GAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGC                               <  SRR19973076.3541171/150‑1 (MQ=60)
                                                                                                                     GCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGA                        <  SRR19973076.1051032/150‑1 (MQ=60)
                                                                                                                     GCTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGA                        <  SRR19973076.3216797/150‑1 (MQ=60)
                                                                                                                      CTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGAC                       <  SRR19973076.4355061/150‑1 (MQ=60)
                                                                                                                      CTTTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGAC                       <  SRR19973076.4354999/150‑1 (MQ=60)
                                                                                                                        TTCGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGG                     >  SRR19973076.3371147/1‑150 (MQ=60)
                                                                                                                          CGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAA                   <  SRR19973076.146003/150‑1 (MQ=60)
                                                                                                                          CGTACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATATCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAA                   <  SRR19973076.315895/150‑1 (MQ=60)
                                                                                                                           GTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCTGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAA                  <  SRR19973076.3697465/150‑1 (MQ=60)
                                                                                                                           GTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAA                  >  SRR19973076.1007770/1‑150 (MQ=60)
                                                                                                                           GTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACGGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCTGAATAACAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAA                  <  SRR19973076.3697466/150‑1 (MQ=60)
                                                                                                                             ACCATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACG                <  SRR19973076.2981186/150‑1 (MQ=60)
                                                                                                                              CCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGC               >  SRR19973076.3030773/1‑150 (MQ=60)
                                                                                                                               CATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCT              <  SRR19973076.2409943/150‑1 (MQ=60)
                                                                                                                               CATTCCCCGCCCGCTGGAGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCT              <  SRR19973076.2596926/150‑1 (MQ=60)
                                                                                                                                   CCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGG          >  SRR19973076.2386514/1‑150 (MQ=60)
                                                                                                                                   CCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGG          >  SRR19973076.2386517/1‑150 (MQ=60)
                                                                                                                                   CCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGG          >  SRR19973076.2386532/1‑150 (MQ=60)
                                                                                                                                   CCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCAATACATTATTCGCCATCTGACGGAAACGCTGGGG          >  SRR19973076.2386523/1‑150 (MQ=60)
                                                                                                                                           GCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAA  <  SRR19973076.3219515/150‑1 (MQ=60)
                                                                                                                                                |                                                                                                                                                
ATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTACTGATGCGCGTTATCAGCGACCTACCGCTGCGCCTGCGCTGGCAGAATAAAAGCCGTGACATCCATTACATTATTCGCCATCTGACGGAAACGCTGGGGACAGACAA  >  CP009273/4208798‑4209086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑