Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 666,998 G→T 8.6% L22I (CTT→ATT)  holA ← DNA polymerase III, delta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273666,9980GT 8.6% 874.6 / 45.4 243L22I (CTT→ATT) holADNA polymerase III, delta subunit
Reads supporting (aligned to +/- strand):  ref base G (128/94);  new base T (12/9);  total (140/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

Only 100 of 247 total aligned reads displayed.

CATAGCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAAT  >  CP009273/666849‑667144
                                                                                                                                                     |                                                                                                                                                  
 aTAGCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGa                                                                                                                                                   >  2:285145/1‑150 (MQ=255)
  tAGCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGAt                                                                                                                                                  <  2:3978824/150‑1 (MQ=255)
  tAGCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGAt                                                                                                                                                  <  2:4445471/150‑1 (MQ=255)
   aGCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATa                                                                                                                                                 <  1:204473/150‑1 (MQ=255)
   aGCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATa                                                                                                                                                 <  2:3049020/150‑1 (MQ=255)
    gCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATAc                                                                                                                                                <  1:2284760/150‑1 (MQ=255)
      cTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATAcgc                                                                                                                                              <  2:885897/150‑1 (MQ=255)
      cTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATAcgc                                                                                                                                              >  1:4056552/1‑150 (MQ=255)
       tGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATAcgcc                                                                                                                                             <  2:3655049/150‑1 (MQ=255)
       tGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATAcgcc                                                                                                                                             <  1:3721135/150‑1 (MQ=255)
              aCGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCa                                                                                                                                      <  1:1046940/150‑1 (MQ=255)
                gAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGc                                                                                                                                    >  2:4410801/1‑150 (MQ=255)
                gAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGc                                                                                                                                    >  2:2645694/1‑150 (MQ=255)
                gAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGc                                                                                                                                    >  2:2136362/1‑150 (MQ=255)
                gAAAAGATCGCATTCCAGTCAGTGTGGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGc                                                                                                                                    >  2:3642317/1‑150 (MQ=255)
                  aaaGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGTAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGccc                                                                                                                                  >  2:4291014/1‑150 (MQ=255)
                  aaaGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCCGccc                                                                                                                                  >  1:43541/1‑150 (MQ=255)
                   aaGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCt                                                                                                                                 <  2:1321316/150‑1 (MQ=255)
                     gATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTc                                                                                                                               <  2:3323249/150‑1 (MQ=255)
                       tCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAt                                                                                                                             <  1:3836552/150‑1 (MQ=255)
                       tCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAt                                                                                                                             >  1:2927608/1‑150 (MQ=255)
                          cATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGa                                                                                                                          >  1:2593156/1‑150 (MQ=255)
                             tCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCt                                                                                                                       <  2:1752562/150‑1 (MQ=255)
                                agtcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTgcg                                                                                                                    <  1:1079179/150‑1 (MQ=255)
                                 gtcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCCGCCCGTCATTGAGCGgcgc                                                                                                                   >  1:423513/1‑150 (MQ=255)
                                 gtcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGTGCTgcgc                                                                                                                   >  1:421619/1‑150 (MQ=255)
                                   cagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTgcgcgc                                                                                                                 >  2:3192372/1‑150 (MQ=255)
                                    agtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCCGCCCGTCAATTAGCTGcgcgcg                                                                                                                >  1:3457000/1‑150 (MQ=255)
                                         tGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAgtt                                                                                                           >  1:3017073/1‑150 (MQ=255)
                                         tGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAgtt                                                                                                           <  1:3056203/150‑1 (MQ=255)
                                             aTCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCCTTGAGCTGCGCGCGGAggtgtta                                                                                                       >  1:3152577/1‑149 (MQ=255)
                                              tCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTcc                                                                                                      <  1:3598767/150‑1 (MQ=255)
                                               cAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCAATGAGCTGCGCGCGGAGTTGTTACg                                                                                                     >  1:2757780/1‑150 (MQ=255)
                                                aaTGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAATGAGCTGCGCGCGGAGTTGTTCCgg                                                                                                    >  1:4435426/1‑150 (MQ=255)
                                                 aTGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCCTTGAGCTGCGCGCGGAGTTGTTCCggg                                                                                                   >  2:3090264/1‑150 (MQ=255)
                                                  tGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCAATGAGCTGCGCGCGGAGTTGTTCCGGGt                                                                                                  >  2:4161262/1‑150 (MQ=255)
                                                   ggAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTa                                                                                                 >  1:2597588/1‑150 (MQ=255)
                                                   ggAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCACAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTa                                                                                                 >  2:2864892/1‑150 (MQ=255)
                                                     aaaaaGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACa                                                                                               >  2:2017116/1‑150 (MQ=255)
                                                         aGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCg                                                                                           >  2:1036134/1‑150 (MQ=255)
                                                           tgtgGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCCGCCCTACATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAAACGaa                                                                                         >  2:189305/1‑150 (MQ=255)
                                                             tggtgTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAatc                                                                                       <  1:4536036/150‑1 (MQ=255)
                                                             tggtgTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAatc                                                                                       >  2:3004464/1‑150 (MQ=255)
                                                             tggtgTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAatc                                                                                       >  1:3402233/1‑150 (MQ=255)
                                                              ggtgTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAatca                                                                                      <  1:2023746/150‑1 (MQ=255)
                                                                  ttcttcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCAGCGCGCGGAGTTGTTCCGGGTAAAAACGAAACATCAg                                                                                  >  1:2803592/1‑150 (MQ=255)
                                                                   tcttcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGt                                                                                 <  1:1910872/150‑1 (MQ=255)
                                                                    cttcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGtt                                                                                <  2:3935197/150‑1 (MQ=255)
                                                                     ttcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTa                                                                               >  1:3301012/1‑150 (MQ=255)
                                                                     ttcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTa                                                                               <  1:160236/150‑1 (MQ=255)
                                                                        gAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTAccc                                                                            <  1:4091951/150‑1 (MQ=255)
                                                                        gAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTAccc                                                                            <  1:1965384/150‑1 (MQ=255)
                                                                        gAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTGACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCGGCGCGCGGAGTTGTTCCGGGTACAACCGACTCATCAGTTAccc                                                                            >  2:3214369/1‑150 (MQ=255)
                                                                         aaTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTATAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCa                                                                           <  2:4297076/150‑1 (MQ=255)
                                                                         aaTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCAATGAGCTGCGCGCGGGGTTGTTCCGGGGACAACCCAATCATCAGTTACCCa                                                                           >  1:3217325/1‑150 (MQ=255)
                                                                          aTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAg                                                                          <  1:4473007/150‑1 (MQ=255)
                                                                            ccTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTACGGGTACAACCGAATCATCAGTTACCCAGCg                                                                        >  2:3444423/1‑150 (MQ=255)
                                                                            ccTTGGGCCGCCGCGACCGGACGAACAGCGTCCTGGCGTTCCTGCACTACCAGAGGATCGGTACCACGTCACAGATACGCCGCGCGCAGCCCTTCAGTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTGACCCAGCg                                                                        <  2:2331121/150‑1 (MQ=255)
                                                                              ttGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCgtg                                                                      <  1:626656/150‑1 (MQ=255)
                                                                               tgtgCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCgtgg                                                                     <  2:699046/150‑1 (MQ=255)
                                                                                gtgCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCgtggt                                                                    <  2:946004/150‑1 (MQ=255)
                                                                                 tgCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAGTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCgtggtg                                                                   <  2:1874253/150‑1 (MQ=255)
                                                                                   ccGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTGATTGAGCAGCGCGGGGAGTTGTTCCGGGTAAAACCGAATACTAAGTTTCCCAGCGGGGTGga                                                                 >  1:940840/1‑150 (MQ=255)
                                                                                      cAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTTAGCTGCGCGCGGAGTTTTTGCGGGTACAAACGAATCATCAGTTACCCAGCGGGGTGGAGAc                                                              >  1:2385553/1‑150 (MQ=255)
                                                                                      cAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAGTGAGCTGCGCGCGGAGTTGTTCCGGGGACAACCGAATACTCAGTTACCCAGCGGGGTGGAGAc                                                              >  1:2433693/1‑150 (MQ=255)
                                                                                       aGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCCGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGGACAACCGAATCAGCAGTTACCCAGCGGGGTGGAGAcg                                                             >  2:1463747/1‑150 (MQ=255)
                                                                                         cTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGAcgcg                                                           >  2:1123189/1‑150 (MQ=255)
                                                                                           aCCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTg                                                         <  1:2975129/150‑1 (MQ=255)
                                                                                                   aaCAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGTGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGCGTCGCGTGCAGGCggt                                                 >  2:4150069/1‑148 (MQ=255)
                                                                                                   aaCAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCATCAATTAGCGGCGCGCGGAGTTGTTCCCGGTACAACCGAAAAAAAAGTTAACCAGCGGGGTGGAGACGCGTGCAGGCGtt                                                 >  1:1837688/1‑150 (MQ=255)
                                                                                                     cAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGc                                               <  2:1362138/150‑1 (MQ=255)
                                                                                                             tGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGGGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTACGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTAt                                       >  1:701323/1‑150 (MQ=255)
                                                                                                             tGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGGGCAGCCCATCATTGTGCTGCGCGCGGGGTTGTTACGGGTACAACCGAATAATCCGTTAACCAGCGTGGTGGTGACGCGTGCAGGGGTTGGCGGTGTAt                                       >  1:4295127/1‑150 (MQ=255)
                                                                                                               gCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTGATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAAACAACAGTTACCCAGCGTGGTGGAGAACCGTGCACGCGTTGCCGGAGTATcc                                     >  2:827548/1‑150 (MQ=255)
                                                                                                                cTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCg                                    <  1:1471197/150‑1 (MQ=255)
                                                                                                                      tGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTgg                              <  1:1430351/150‑1 (MQ=255)
                                                                                                                        cAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAAACAACAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTc                            >  1:1787157/1‑150 (MQ=255)
                                                                                                                          aTAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGa                          <  2:1178636/150‑1 (MQ=255)
                                                                                                                           tAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGAc                         <  1:3956965/150‑1 (MQ=255)
                                                                                                                           tAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGAc                         <  1:3309900/150‑1 (MQ=255)
                                                                                                                           tAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGAc                         <  2:2254914/150‑1 (MQ=255)
                                                                                                                            aaCAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACg                        <  1:468001/150‑1 (MQ=255)
                                                                                                                            aaCAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACg                        >  2:4067427/1‑150 (MQ=255)
                                                                                                                            aaCAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATAAGTTACCCAGCGGGGTGGAGACGCGTGCAGGCGGTGCCGGAGTATACGTTGTGGTCGACg                        >  1:2186804/1‑150 (MQ=255)
                                                                                                                            aaCAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGAGTTGTTACGGGTACAAACGAATCATCAGTTACCCACCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACg                        >  1:1015974/1‑150 (MQ=255)
                                                                                                                             aCAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCCTCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGt                       >  2:3001461/1‑150 (MQ=255)
                                                                                                                             aCAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTACGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGt                       >  2:4411816/1‑150 (MQ=255)
                                                                                                                               agagGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGTGCTGCGCGCGGAGGTGTTCCGGGTACAACCGCATCATCAGTTAACCAGCGTGGGGGAGAAGCGTGCAGGCGGTGCCGGAGTATCCGGTGTGGTCGACGTCt                     >  1:234217/1‑150 (MQ=255)
                                                                                                                               agagGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCt                     <  2:4498495/150‑1 (MQ=255)
                                                                                                                               agagGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCt                     <  2:2355959/150‑1 (MQ=255)
                                                                                                                                gagGATCGTTACCAAGTAAAAGATACGCCGCGCGCCGCCCTTCCTTGTGCGGGGCGCGGCGTTGTTGCGGGTACAACCCAAACATCAGGTACCCACCCGGGGGGAGGCGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTg                    >  1:3517544/1‑150 (MQ=255)
                                                                                                                                gagGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTg                    <  2:1462443/150‑1 (MQ=255)
                                                                                                                                gagGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCATAATTTATATGCGCGCGGAGTTTTTCACGGTACAACCCAATCAAAAGTTACCCAGCGTGGTGGGGACGCGTGCAGGCGTTTCCCGAGTATCCGTTGTGGTCGACGGCTg                    >  1:2587981/1‑150 (MQ=255)
                                                                                                                                 agGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGTGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGt                   <  2:2679973/150‑1 (MQ=255)
                                                                                                                                 agGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTTCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGt                   >  1:1864846/1‑150 (MQ=255)
                                                                                                                                 agGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGt                   <  1:2496548/150‑1 (MQ=255)
                                                                                                                                       gTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTtcg             <  2:507415/150‑1 (MQ=255)
                                                                                                                                           ccAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTATTCGTCgg         >  2:498772/1‑150 (MQ=255)
                                                                                                                                              aGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGGGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTATTCGTCGGAAc      >  2:1618858/1‑150 (MQ=255)
                                                                                                                                                 aaaaTATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGaa   <  1:865897/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CATAGCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAAT  >  CP009273/666849‑667144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A