Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 666,998 G→T 35.2% L22I (CTT→ATT)  holA ← DNA polymerase III, delta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273666,9980GT35.2% 380.1 / 216.3 237L22I (CTT→ATT) holADNA polymerase III, delta subunit
Reads supporting (aligned to +/- strand):  ref base G (88/65);  new base T (44/39);  total (133/104)
Fisher's exact test for biased strand distribution p-value = 5.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

CATAGCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATA  >  CP009273/666849‑667145
                                                                                                                                                     |                                                                                                                                                   
cATAGCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAg                                                                                                                                                     <  2:2815496/150‑1 (MQ=255)
     ccTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATAcg                                                                                                                                                >  2:2709766/1‑150 (MQ=255)
        ggCATAACGAAAAGATCGCATTCCAGTCAGTGTTTGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATAcgccg                                                                                                                                             <  2:1310126/150‑1 (MQ=255)
          cATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCcgcg                                                                                                                                           >  2:2433651/1‑150 (MQ=255)
          cATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCcgcg                                                                                                                                           >  1:3093192/1‑150 (MQ=255)
          cATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCcgcg                                                                                                                                           >  1:564472/1‑150 (MQ=255)
           aTAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCcgcgc                                                                                                                                          >  2:2918077/1‑150 (MQ=255)
           aTAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCcgcgc                                                                                                                                          >  2:177974/1‑150 (MQ=255)
           aTAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCcgcgc                                                                                                                                          >  1:443351/1‑150 (MQ=255)
            tAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCcgcgcg                                                                                                                                         >  2:452907/1‑150 (MQ=255)
             aaCGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAAGGGAAAAAGTGTGGTGTTCTTCGAATCCGTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCACTAACAGAGGATCGGTACCAAGTCAAATATACGCCgcgcgc                                                                                                                                        >  2:3356499/1‑150 (MQ=255)
              aCGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCGACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCa                                                                                                                                       <  2:250470/150‑1 (MQ=255)
              aCGAAAAGATCGCATTCCAGTCAGTGTTGGGAGCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCa                                                                                                                                       <  2:2332312/150‑1 (MQ=255)
                gAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGc                                                                                                                                     >  2:1657740/1‑150 (MQ=255)
                gAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGc                                                                                                                                     >  1:1263361/1‑150 (MQ=255)
                 aaaaGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGcc                                                                                                                                    >  1:2227129/1‑150 (MQ=255)
                    aGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCtt                                                                                                                                 <  2:1029700/150‑1 (MQ=255)
                     gATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTc                                                                                                                                <  2:638080/150‑1 (MQ=255)
                     gATCGCATCCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTc                                                                                                                                >  2:1714143/1‑150 (MQ=255)
                        cGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAtt                                                                                                                             >  1:3303600/1‑150 (MQ=255)
                        cGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTATCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCAtt                                                                                                                             >  1:3441124/1‑150 (MQ=255)
                        cGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGGGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGCTCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTGCAtt                                                                                                                             >  2:2802572/1‑150 (MQ=255)
                          cATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGa                                                                                                                           >  2:1113567/1‑150 (MQ=255)
                           aTTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAg                                                                                                                          <  2:3017513/150‑1 (MQ=255)
                            ttCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGc                                                                                                                         >  2:1837061/1‑150 (MQ=255)
                             tCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCt                                                                                                                        <  1:2583552/150‑1 (MQ=255)
                             tCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCt                                                                                                                        >  2:3904798/1‑150 (MQ=255)
                              ccAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTg                                                                                                                       <  1:2827308/150‑1 (MQ=255)
                              ccAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTg                                                                                                                       <  1:4028763/150‑1 (MQ=255)
                               cagtcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTgc                                                                                                                      <  2:2543379/150‑1 (MQ=255)
                                agtcagtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTgcg                                                                                                                     <  1:1463533/150‑1 (MQ=255)
                                    agtGTTGGGATCAATGGAAAAAGTGTGGTGTTCTGCGAATCCTGGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCAGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCCGCCCTTCATTGAGCTGcgcgcg                                                                                                                 <  2:2265679/150‑1 (MQ=255)
                                     gtgtTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCgg                                                                                                                <  1:1394997/150‑1 (MQ=255)
                                         tGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAgtt                                                                                                            <  2:2070658/150‑1 (MQ=255)
                                          gggATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAgttg                                                                                                           <  1:3594495/150‑1 (MQ=255)
                                             aTCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTc                                                                                                        >  1:2242389/1‑150 (MQ=255)
                                              tCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGAGTTGTTcc                                                                                                       >  1:1801213/1‑150 (MQ=255)
                                               cAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCg                                                                                                      >  1:1672772/1‑150 (MQ=255)
                                               cAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCg                                                                                                      <  2:74568/150‑1 (MQ=255)
                                                aaTGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCgg                                                                                                     <  2:227267/150‑1 (MQ=255)
                                                 aTGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCggg                                                                                                    >  1:3740344/1‑150 (MQ=255)
                                                    gAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTAc                                                                                                 >  1:700831/1‑150 (MQ=255)
                                                      aaaaGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACaa                                                                                               >  2:793324/1‑150 (MQ=255)
                                                       aaaGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAAc                                                                                              >  2:3805167/1‑150 (MQ=255)
                                                        aaGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAAcc                                                                                             >  1:3649772/1‑150 (MQ=255)
                                                                tgtTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAatcatc                                                                                     <  1:2285846/150‑1 (MQ=255)
                                                                tgtTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCGTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAatcatc                                                                                     >  1:3915423/1‑150 (MQ=255)
                                                                 gtTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAAtcatca                                                                                    >  2:1563268/1‑150 (MQ=255)
                                                                   tcttcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGt                                                                                  >  2:4057415/1‑150 (MQ=255)
                                                                   tcttcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGt                                                                                  >  1:1045875/1‑150 (MQ=255)
                                                                    cttcGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGtt                                                                                 >  2:1346154/1‑150 (MQ=255)
                                                                        gAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTAccc                                                                             >  1:213658/1‑150 (MQ=255)
                                                                          aTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAg                                                                           >  1:3361980/1‑150 (MQ=255)
                                                                          aTCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAg                                                                           >  2:3668722/1‑150 (MQ=255)
                                                                                   ccGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGga                                                                  >  1:2061288/1‑150 (MQ=255)
                                                                                    cGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGgag                                                                 >  1:2909458/1‑150 (MQ=255)
                                                                                     gCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGgaga                                                                >  1:1649416/1‑150 (MQ=255)
                                                                                           aCCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTg                                                          <  2:937052/150‑1 (MQ=255)
                                                                                            ccTGCCGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGc                                                         <  1:3141678/150‑1 (MQ=255)
                                                                                              tGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATNAGTTACCCAGCGTGGTGGAGACGCGTGCAg                                                       <  1:3854327/150‑1 (MQ=255)
                                                                                               gACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGGGGTGGAGACGCGTGCAgg                                                      >  2:2001052/1‑150 (MQ=255)
                                                                                                aCGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGc                                                     <  2:3648404/150‑1 (MQ=255)
                                                                                                 cGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCg                                                    >  2:1733281/1‑150 (MQ=255)
                                                                                                  gAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGt                                                   >  2:166505/1‑150 (MQ=255)
                                                                                                   aaCAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGtt                                                  <  1:749541/150‑1 (MQ=255)
                                                                                                   aaCAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGtt                                                  <  1:2461570/150‑1 (MQ=255)
                                                                                                   aaCAGCGTCCTGGCTTTCCTGCAATAACAGAGGAGCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTGCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGGGGTGGAGACGCGTGCAGGCGtt                                                  >  2:1322542/1‑150 (MQ=255)
                                                                                                     cAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGc                                                >  2:2629435/1‑150 (MQ=255)
                                                                                                     cAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGc                                                <  2:145766/150‑1 (MQ=255)
                                                                                                      aGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGcc                                               >  1:1347678/1‑150 (MQ=255)
                                                                                                         gTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGa                                            >  1:2876335/1‑150 (MQ=255)
                                                                                                           ccTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGt                                          <  2:2126481/150‑1 (MQ=255)
                                                                                                             tGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTAt                                        <  2:604139/150‑1 (MQ=255)
                                                                                                               gCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCCTCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATcc                                      >  2:891229/1‑150 (MQ=255)
                                                                                                                cTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCATCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTTCCGGAGTATCCg                                     >  2:1246946/1‑150 (MQ=255)
                                                                                                                 tttCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCGCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGt                                    >  2:436693/1‑150 (MQ=255)
                                                                                                                  ttCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGtt                                   <  1:896120/150‑1 (MQ=255)
                                                                                                                  ttCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGtt                                   <  1:3378712/150‑1 (MQ=255)
                                                                                                                   tCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGGtg                                  >  1:1549403/1‑150 (MQ=255)
                                                                                                                        cAATAACAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTc                             <  1:188738/150‑1 (MQ=255)
                                                                                                                        cAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTc                             >  2:3653050/1‑150 (MQ=255)
                                                                                                                        cAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTAACCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATACGGTGTGGTc                             >  1:1077053/1‑150 (MQ=255)
                                                                                                                             aCAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGt                        >  2:644896/1‑150 (MQ=255)
                                                                                                                              cAGAGGATCGTTACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGATGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTc                       >  2:2384322/1‑150 (MQ=255)
                                                                                                                                  ggATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGtt                   <  2:2976996/150‑1 (MQ=255)
                                                                                                                                   gATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGttc                  >  1:974426/1‑150 (MQ=255)
                                                                                                                                    agcggtACCAAGTCAAAGATACGCCGCTCGCAGCCCTTAATTGATCTGAGCGCTGAGTTGTTCCGTGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCTGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTATGttct                 <  2:3166604/145‑1 (MQ=255)
                                                                                                                                     tCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGttctt                <  2:3053862/150‑1 (MQ=255)
                                                                                                                                      cGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGttcttc               <  1:1317474/150‑1 (MQ=255)
                                                                                                                                       gTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTtcg              >  2:2755308/1‑150 (MQ=255)
                                                                                                                                        ttACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTtcgt             >  2:1491638/1‑150 (MQ=255)
                                                                                                                                        ttACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTtcgt             <  1:455656/150‑1 (MQ=255)
                                                                                                                                         tACCAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTtcgtc            >  2:3805905/1‑150 (MQ=255)
                                                                                                                                           ccAAGTAAAATATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCgg          <  1:1686408/150‑1 (MQ=255)
                                                                                                                                             aaGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGaa        <  2:3977189/150‑1 (MQ=255)
                                                                                                                                              aGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAAc       >  1:3252594/1‑150 (MQ=255)
                                                                                                                                              aGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAAACATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAAc       >  1:3844453/1‑150 (MQ=255)
                                                                                                                                               gTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTACCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACg      <  2:3527539/150‑1 (MQ=255)
                                                                                                                                                  aaaTATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAat   <  2:2286474/150‑1 (MQ=255)
                                                                                                                                                  aaaGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAat   <  1:824059/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CATAGCCTGGCATAACGAAAAGATCGCATTCCAGTCAGTGTTGGGATCAATGGAAAAAGTGTGGTGTTCTTCGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATA  >  CP009273/666849‑667145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A