Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,548,144 G→T 89.5% intergenic (‑49/+85) adhP ← / ← maeA ethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, (decarboxylating, NAD‑requiring) (malic enzyme)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,548,1440GT89.5% 590.0 / 61.3 206intergenic (‑49/+85)adhP/maeAethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, (decarboxylating, NAD‑requiring) (malic enzyme)
Reads supporting (aligned to +/- strand):  ref base G (10/12);  new base T (91/93);  total (101/105)
Fisher's exact test for biased strand distribution p-value = 8.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 210 total aligned reads displayed.

ACACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTT  >  CP009273/1547997‑1548293
                                                                                                                                                   |                                                                                                                                                     
 cacTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATAtt                                                                                                                                                    <  2:1480335/150‑1 (MQ=255)
   cTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTa                                                                                                                                                  <  1:1103480/150‑1 (MQ=255)
   cTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTa                                                                                                                                                  <  2:4289805/150‑1 (MQ=255)
    tCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTAt                                                                                                                                                 >  1:1022443/1‑150 (MQ=255)
     ccATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTAtt                                                                                                                                                >  1:3429530/1‑150 (MQ=255)
       aTTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCg                                                                                                                                              >  2:2517545/1‑150 (MQ=255)
            cagcagGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACt                                                                                                                                         >  1:1544216/1‑150 (MQ=255)
            cagcagGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACt                                                                                                                                         >  2:1831797/1‑150 (MQ=255)
             agcagGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTa                                                                                                                                        >  2:126312/1‑150 (MQ=255)
              gcagGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTCACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTAc                                                                                                                                       <  2:4339483/150‑1 (MQ=255)
               cagGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTAcc                                                                                                                                      >  1:2789842/1‑150 (MQ=255)
                agGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTAccc                                                                                                                                     >  1:2153679/1‑150 (MQ=255)
                 gggCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCa                                                                                                                                    >  1:3533831/1‑150 (MQ=255)
                      tCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATg                                                                                                                               <  1:2205301/150‑1 (MQ=255)
                       cGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATgg                                                                                                                              <  1:968910/150‑1 (MQ=255)
                       cGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATgg                                                                                                                              >  2:1316370/1‑150 (MQ=255)
                         ccATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGTTGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGAt                                                                                                                            <  2:1003469/150‑1 (MQ=255)
                         ccATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGAt                                                                                                                            >  2:1180831/1‑150 (MQ=255)
                          cATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATg                                                                                                                           >  1:3631012/1‑150 (MQ=255)
                                 cAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTg                                                                                                                    <  2:3675162/150‑1 (MQ=255)
                                 cAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTg                                                                                                                    <  1:4016081/150‑1 (MQ=255)
                                   gTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGaa                                                                                                                  >  2:90950/1‑150 (MQ=255)
                                     gAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGaaaa                                                                                                                <  1:4463754/150‑1 (MQ=255)
                                         gcAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAggg                                                                                                            >  1:1027403/1‑150 (MQ=255)
                                           aGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGgag                                                                                                          <  1:1316370/150‑1 (MQ=255)
                                              gtTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAgg                                                                                                       <  1:1461027/150‑1 (MQ=255)
                                               ttttATACGTACCGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTGTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAggg                                                                                                      <  2:1376142/150‑1 (MQ=255)
                                               ttttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGagagag                                                                                                      <  2:218242/150‑3 (MQ=255)
                                               ttttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAggg                                                                                                      >  1:2011661/1‑150 (MQ=255)
                                               ttttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAggg                                                                                                      <  2:1723725/150‑1 (MQ=255)
                                                tttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGa                                                                                                     <  2:604092/150‑1 (MQ=255)
                                                tttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGa                                                                                                     <  1:932643/150‑1 (MQ=255)
                                                tttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGa                                                                                                     <  1:1738839/150‑1 (MQ=255)
                                                   ataCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAAt                                                                                                  >  1:4259411/1‑150 (MQ=255)
                                                    taCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATa                                                                                                 >  1:2609263/1‑150 (MQ=255)
                                                    taCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATa                                                                                                 <  1:1831173/150‑1 (MQ=255)
                                                      cGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGc                                                                                               <  1:3552750/150‑1 (MQ=255)
                                                         aaCGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCg                                                                                            <  1:1227519/150‑1 (MQ=255)
                                                         aaCGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCg                                                                                            >  2:1106791/1‑150 (MQ=255)
                                                         aaCGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCg                                                                                            >  2:2186429/1‑150 (MQ=255)
                                                         aaCGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCg                                                                                            >  1:3478416/1‑150 (MQ=255)
                                                           cGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGt                                                                                          >  2:3698195/1‑150 (MQ=255)
                                                           cGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGt                                                                                          >  1:2500497/1‑150 (MQ=255)
                                                            gTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGACATAGCCCGGTa                                                                                         >  2:2401889/1‑150 (MQ=255)
                                                            gTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGtc                                                                                         >  2:3934364/1‑149 (MQ=255)
                                                             tCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAg                                                                                        <  1:3113866/150‑1 (MQ=255)
                                                              cAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGc                                                                                       >  2:1598231/1‑150 (MQ=255)
                                                              cAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGc                                                                                       >  2:1283352/1‑150 (MQ=255)
                                                                aCATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCt                                                                                     <  1:4136272/150‑1 (MQ=255)
                                                                aCATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCt                                                                                     <  2:2982975/150‑1 (MQ=255)
                                                                aCATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCt                                                                                     <  1:11791/150‑1 (MQ=255)
                                                                  atgatgATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTc                                                                                   >  1:1013615/1‑150 (MQ=255)
                                                                     atgatCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGTTGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACt                                                                                <  1:894684/150‑1 (MQ=255)
                                                                       gatCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTAc                                                                              >  2:2013409/1‑150 (MQ=255)
                                                                        atCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGTATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTAcc                                                                             >  1:2743592/1‑150 (MQ=255)
                                                                        atCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTAcc                                                                             <  2:3631012/150‑1 (MQ=255)
                                                                         tCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCg                                                                            <  1:2745624/150‑1 (MQ=255)
                                                                         tCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCg                                                                            <  2:3947286/150‑1 (MQ=255)
                                                                               gTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCa                                                                      <  1:588211/150‑1 (MQ=255)
                                                                               gTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCa                                                                      >  1:988287/1‑150 (MQ=255)
                                                                                tAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAg                                                                     >  1:1659089/1‑150 (MQ=255)
                                                                                       cgCAGCCTTCAGAGGTCCTCCTGGTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGa                                                              <  2:2604131/149‑1 (MQ=255)
                                                                                            ccTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAg                                                         >  2:1246957/1‑150 (MQ=255)
                                                                                             cTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAgg                                                        >  1:2045668/1‑150 (MQ=255)
                                                                                             cTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAgg                                                        >  1:3995752/1‑150 (MQ=255)
                                                                                               tCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTa                                                      <  2:438730/150‑1 (MQ=255)
                                                                                                 aTAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTAcg                                                    <  2:1544203/150‑1 (MQ=255)
                                                                                                   aGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTAcggc                                                  <  1:4395988/150‑1 (MQ=255)
                                                                                                    gTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGATATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTAcggcg                                                 <  1:1152591/150‑1 (MQ=255)
                                                                                                    gTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTAcggcg                                                 >  1:2285643/1‑150 (MQ=255)
                                                                                                    gTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTAcggcg                                                 <  2:4317543/150‑1 (MQ=255)
                                                                                                    gTTCCTCCTGTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTAcggcg                                                 <  2:3891314/150‑1 (MQ=255)
                                                                                                     ttCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTAcggcgg                                                >  2:3567426/1‑150 (MQ=255)
                                                                                                       cctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTa                                              >  2:4504895/1‑150 (MQ=255)
                                                                                                       cctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTa                                              >  2:1264186/1‑150 (MQ=255)
                                                                                                        ctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAg                                             >  1:1903914/1‑150 (MQ=255)
                                                                                                        ctcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAg                                             <  2:1764111/150‑1 (MQ=255)
                                                                                                         tcctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGt                                            >  1:3664573/1‑150 (MQ=255)
                                                                                                           ctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTcg                                          <  2:2861287/150‑1 (MQ=255)
                                                                                                           ctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTcg                                          >  1:4489796/1‑150 (MQ=255)
                                                                                                           catttCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTcg                                          <  2:852643/148‑1 (MQ=255)
                                                                                                                 ggATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAt                                    >  2:2059267/1‑150 (MQ=255)
                                                                                                                 ggATGATGTTATGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAt                                    <  1:3003212/150‑1 (MQ=255)
                                                                                                                  gatgatGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAtt                                   <  2:49451/150‑1 (MQ=255)
                                                                                                                   atgatgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTc                                  <  2:2925471/150‑1 (MQ=255)
                                                                                                                      atgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGc                               >  2:377055/1‑150 (MQ=255)
                                                                                                                      atgTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGc                               <  1:1396466/150‑1 (MQ=255)
                                                                                                                           cTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGcc                          <  1:2771930/150‑1 (MQ=255)
                                                                                                                                  gCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAtt                   <  2:3762147/150‑1 (MQ=255)
                                                                                                                                   cAGGTGAGGCAAATGGTATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTa                  <  2:2753638/150‑1 (MQ=255)
                                                                                                                                    aGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAt                 <  1:3262393/150‑1 (MQ=255)
                                                                                                                                      gTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCg               >  1:633662/1‑150 (MQ=255)
                                                                                                                                      gTGAGGCAAATGATATTTATTCGCCACTACCCAGAATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCg               <  2:4140470/150‑1 (MQ=255)
                                                                                                                                       tGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGt              <  2:354419/150‑1 (MQ=255)
                                                                                                                                       tGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGt              <  2:1933065/150‑1 (MQ=255)
                                                                                                                                        gAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTc             <  2:1704923/150‑1 (MQ=255)
                                                                                                                                         aGGCAAATGAGATTTATGCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCa            <  2:3263824/150‑1 (MQ=255)
                                                                                                                                               aTGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGcc      <  1:245134/150‑1 (MQ=255)
                                                                                                                                                tGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCt     >  2:1363359/1‑150 (MQ=255)
                                                                                                                                                tGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCt     <  2:1022443/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTT  >  CP009273/1547997‑1548293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTT  >  CP009273/1547999‑1548293
                                                                                                                                                 |                                                                                                                                                     
ACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTT                                                                                                                                                   >  SRR19973080.1863849/1‑150 (MQ=60)
 CTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTA                                                                                                                                                  <  SRR19973080.1109818/150‑1 (MQ=60)
  TCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTAT                                                                                                                                                 >  SRR19973080.1028399/1‑150 (MQ=60)
  TCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTAT                                                                                                                                                 >  SRR19973080.1028523/1‑150 (MQ=60)
   CCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATT                                                                                                                                                >  SRR19973080.3448199/1‑150 (MQ=60)
          CAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACT                                                                                                                                         >  SRR19973080.1553155/1‑150 (MQ=60)
          CAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACT                                                                                                                                         >  SRR19973080.1553142/1‑150 (MQ=60)
             CAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACC                                                                                                                                      >  SRR19973080.2805233/1‑150 (MQ=60)
              AGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCC                                                                                                                                     >  SRR19973080.2165731/1‑150 (MQ=60)
              AGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCC                                                                                                                                     >  SRR19973080.2165730/1‑150 (MQ=60)
               GGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCA                                                                                                                                    >  SRR19973080.3553377/1‑150 (MQ=60)
                    TCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATG                                                                                                                               <  SRR19973080.2217517/150‑1 (MQ=60)
                     CGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGG                                                                                                                              <  SRR19973080.974774/150‑1 (MQ=60)
                       CCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGAT                                                                                                                            <  SRR19973080.1178236/150‑1 (MQ=60)
                        CATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATG                                                                                                                           >  SRR19973080.3651073/1‑150 (MQ=60)
                         ATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGA                                                                                                                          >  SRR19973080.1068101/1‑150 (MQ=60)
                         ATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGA                                                                                                                          <  SRR19973080.1679759/150‑1 (MQ=60)
                               CAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTG                                                                                                                    <  SRR19973080.4038109/150‑1 (MQ=60)
                               CAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTG                                                                                                                    <  SRR19973080.4305085/150‑1 (MQ=60)
                               CAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTG                                                                                                                    <  SRR19973080.1751067/150‑1 (MQ=60)
                                  TGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAA                                                                                                                 <  SRR19973080.1386510/150‑1 (MQ=60)
                                   GAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAA                                                                                                                <  SRR19973080.4487937/150‑1 (MQ=60)
                                   GAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAA                                                                                                                <  SRR19973080.4485575/150‑1 (MQ=60)
                                    AGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAA                                                                                                               >  SRR19973080.2925793/1‑150 (MQ=60)
                                       GCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGG                                                                                                            >  SRR19973080.1031886/1‑150 (MQ=60)
                                       GCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGG                                                                                                            >  SRR19973080.1033505/1‑150 (MQ=60)
                                         AGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAG                                                                                                          <  SRR19973080.1324326/150‑1 (MQ=60)
                                          GTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGA                                                                                                         >  SRR19973080.1573734/1‑150 (MQ=60)
                                            GTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGG                                                                                                       <  SRR19973080.3568000/150‑1 (MQ=60)
                                            GTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGG                                                                                                       <  SRR19973080.2411682/150‑1 (MQ=60)
                                            GTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGG                                                                                                       <  SRR19973080.1469803/150‑1 (MQ=60)
                                            GTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGG                                                                                                       <  SRR19973080.1469469/150‑1 (MQ=60)
                                             TTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGG                                                                                                      >  SRR19973080.2023106/1‑150 (MQ=60)
                                             TTTTATAAGTAACGTCAACATGATGAACCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCTCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGG                                                                                                      >  SRR19973080.2023215/1‑150 (MQ=60)
                                              TTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGA                                                                                                     <  SRR19973080.4381769/150‑1 (MQ=60)
                                              TTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGA                                                                                                     <  SRR19973080.1748889/150‑1 (MQ=60)
                                              TTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGA                                                                                                     <  SRR19973080.938345/150‑1 (MQ=60)
                                              TTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGA                                                                                                     <  SRR19973080.938300/150‑1 (MQ=60)
                                                 ATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAAT                                                                                                  >  SRR19973080.4282649/1‑150 (MQ=60)
                                                  TACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATA                                                                                                 <  SRR19973080.317996/150‑1 (MQ=60)
                                                  TACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATA                                                                                                 <  SRR19973080.1841503/150‑1 (MQ=60)
                                                  TACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATA                                                                                                 >  SRR19973080.2623429/1‑150 (MQ=60)
                                                    CGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGC                                                                                               <  SRR19973080.3572398/150‑1 (MQ=60)
                                                       AACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCG                                                                                            >  SRR19973080.3497547/1‑150 (MQ=60)
                                                       AACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCG                                                                                            <  SRR19973080.1234949/150‑1 (MQ=60)
                                                       AACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCG                                                                                            <  SRR19973080.1235170/150‑1 (MQ=60)
                                                         CGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGT                                                                                          >  SRR19973080.2512993/1‑150 (MQ=60)
                                                         CGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGT                                                                                          >  SRR19973080.2514295/1‑150 (MQ=60)
                                                           TCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAG                                                                                        <  SRR19973080.3130977/150‑1 (MQ=60)
                                                              ACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCT                                                                                     <  SRR19973080.4158657/150‑1 (MQ=60)
                                                              ACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCT                                                                                     <  SRR19973080.11934/150‑1 (MQ=60)
                                                                ATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTC                                                                                   >  SRR19973080.1019670/1‑150 (MQ=60)
                                                                   ATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGTTGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACT                                                                                <  SRR19973080.900159/150‑1 (MQ=60)
                                                                    TGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTA                                                                               >  SRR19973080.3360552/1‑150 (MQ=60)
                                                                    TGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCAACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGACCGGTAGCCTGCACTA                                                                               >  SRR19973080.4006496/1‑150 (MQ=60)
                                                                      ATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGTATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACC                                                                             >  SRR19973080.2758696/1‑150 (MQ=60)
                                                                      ATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACC                                                                             >  SRR19973080.2759322/1‑150 (MQ=60)
                                                                       TCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCG                                                                            <  SRR19973080.2760738/150‑1 (MQ=60)
                                                                          TTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGC                                                                         <  SRR19973080.2777305/150‑1 (MQ=60)
                                                                          TTCGTAACAACTGCAGCCTTCATAGGTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGC                                                                         <  SRR19973080.4087903/150‑1 (MQ=60)
                                                                             GTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGTAATAGCCCGGTAGCCTTCACTACCGGGCGCA                                                                      <  SRR19973080.591962/150‑1 (MQ=60)
                                                                             GTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCA                                                                      >  SRR19973080.994240/1‑150 (MQ=60)
                                                                             GTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCA                                                                      <  SRR19973080.591870/150‑1 (MQ=60)
                                                                              TAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAG                                                                     >  SRR19973080.1668790/1‑150 (MQ=60)
                                                                                 CAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCT                                                                  >  SRR19973080.966070/1‑150 (MQ=60)
                                                                                    CTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAG                                                               >  SRR19973080.3521416/1‑150 (MQ=60)
                                                                                     TGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGA                                                              >  SRR19973080.997658/1‑150 (MQ=60)
                                                                                        AGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGG                                                           <  SRR19973080.983121/150‑1 (MQ=60)
                                                                                         GCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGA                                                          >  SRR19973080.2130251/1‑150 (MQ=60)
                                                                                         GCCTTCATAGTTCCTCCTGTTAGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGA                                                          <  SRR19973080.2021601/150‑1 (MQ=60)
                                                                                           CTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGG                                                        >  SRR19973080.4017708/1‑150 (MQ=60)
                                                                                           CTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGG                                                        >  SRR19973080.4017954/1‑150 (MQ=60)
                                                                                           CTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGG                                                        >  SRR19973080.2057284/1‑150 (MQ=60)
                                                                                                 AGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGC                                                  <  SRR19973080.4419925/150‑1 (MQ=60)
                                                                                                 AGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGC                                                  <  SRR19973080.499673/150‑1 (MQ=60)
                                                                                                  GTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGATATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCG                                                 <  SRR19973080.1159392/150‑1 (MQ=60)
                                                                                                  GTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCG                                                 >  SRR19973080.2298213/1‑150 (MQ=60)
                                                                                                  GTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCG                                                 <  SRR19973080.1159364/150‑1 (MQ=60)
                                                                                                      CTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAG                                             >  SRR19973080.1914591/1‑150 (MQ=60)
                                                                                                       TCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT                                            >  SRR19973080.3684723/1‑150 (MQ=60)
                                                                                                       TCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGT                                            >  SRR19973080.3684770/1‑150 (MQ=60)
                                                                                                         CTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCG                                          >  SRR19973080.4514068/1‑150 (MQ=60)
                                                                                                         CTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCG                                          <  SRR19973080.2224010/150‑1 (MQ=60)
                                                                                                              CGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTA                                     <  SRR19973080.2647814/150‑1 (MQ=60)
                                                                                                               GGATGATGTTATGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAT                                    <  SRR19973080.3019373/150‑1 (MQ=60)
                                                                                                                   GATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGG                                <  SRR19973080.4233975/150‑1 (MQ=60)
                                                                                                                    ATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGC                               <  SRR19973080.1404756/150‑1 (MQ=60)
                                                                                                                    ATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGC                               <  SRR19973080.3616506/150‑1 (MQ=60)
                                                                                                                    ATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGC                               <  SRR19973080.1404807/150‑1 (MQ=60)
                                                                                                                         CTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCC                          <  SRR19973080.2787222/150‑1 (MQ=60)
                                                                                                                               AGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAT                    >  SRR19973080.2417169/1‑150 (MQ=60)
                                                                                                                                 CAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTA                  <  SRR19973080.2531409/150‑1 (MQ=60)
                                                                                                                                  AGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAT                 >  SRR19973080.3448675/1‑150 (MQ=60)
                                                                                                                                  AGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAT                 >  SRR19973080.3447549/1‑150 (MQ=60)
                                                                                                                                  AGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAT                 <  SRR19973080.3280328/150‑1 (MQ=60)
                                                                                                                                   GGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTGAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGACATTATC                >  SRR19973080.790275/1‑150 (MQ=60)
                                                                                                                                    GTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCG               >  SRR19973080.637527/1‑150 (MQ=60)
                                                                                                                                     TGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGT              >  SRR19973080.303049/1‑150 (MQ=60)
                                                                                                                                          CAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATG         >  SRR19973080.2792580/1‑150 (MQ=60)
                                                                                                                                             ATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCC      <  SRR19973080.246822/150‑1 (MQ=60)
                                                                                                                                              TGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCT     <  SRR19973080.4495574/150‑1 (MQ=60)
                                                                                                                                                ATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGT   >  SRR19973080.4436901/1‑150 (MQ=60)
                                                                                                                                                ATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGT   >  SRR19973080.4435282/1‑150 (MQ=60)
                                                                                                                                                 TATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTT  >  SRR19973080.1514153/1‑150 (MQ=60)
                                                                                                                                                 GATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTGCACTACCGGGCGCAGGCTGAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTAGCCCGAAATTATCGTCAATGGCCTGTT  >  SRR19973080.1209280/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                     
ACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTT  >  CP009273/1547999‑1548293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑