Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F204 I0 R1	1155	255.8	8356456	95.7%	7997128	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,548,144	G→T	100%	intergenic (‑49/+85)	adhP ← / ← maeA	ethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, (decarboxylating, NAD‑requiring) (malic enzyme)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,548,144	0	G	T	99.2%	820.9 / ‑1.3	236	intergenic (‑49/+85)	adhP/maeA	ethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, (decarboxylating, NAD‑requiring) (malic enzyme)
Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (112/122); total (113/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 237 total aligned reads displayed.

ACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCT  >  CP009273/1547999‑1548290

acTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATAttt                                                                                                                                                >  1:733743/1‑150 (MQ=255)

acTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATAttt                                                                                                                                                <  2:2002246/150‑1 (MQ=255)

acTCCATTTTCAGCAGGGCTTCGCCATGTCTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATAttt                                                                                                                                                >  1:2481626/1‑150 (MQ=255)

        tacagcagGGCGTCGCCATGCTCCCGTGAGCGCAGTGGTTTATACGTACCGTCAACACGCTGATCCATCGTAACAACTGCAGCCTTCATACTTCCCCCTTTACGGCTGATGTTCTGCATCGCAGGTGAGGCAAATGATATTTATTCGCCa                                                                                                                                        <  2:3766525/148‑1 (MQ=255)

         tCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCAc                                                                                                                                       >  2:3967142/1‑150 (MQ=255)

           agcagGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTa                                                                                                                                     >  1:2378136/1‑150 (MQ=255)

           agcagGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTa                                                                                                                                     >  1:3403088/1‑150 (MQ=255)

           agcagGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTa                                                                                                                                     >  2:1332593/1‑150 (MQ=255)

            gcagGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTAc                                                                                                                                    <  1:1450116/150‑1 (MQ=255)

               gggCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCa                                                                                                                                 >  2:1099246/1‑150 (MQ=255)

                ggCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAg                                                                                                                                >  2:3312936/1‑150 (MQ=255)

                ggCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAg                                                                                                                                <  1:2066656/150‑1 (MQ=255)

                ggCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAg                                                                                                                                <  2:1319974/150‑1 (MQ=255)

                ggCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAg                                                                                                                                <  1:1596683/150‑1 (MQ=255)

                ggCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAg                                                                                                                                <  2:2534160/150‑1 (MQ=255)

                  cTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTa                                                                                                                              <  2:4155626/150‑1 (MQ=255)

                  cTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTa                                                                                                                              <  2:1841988/150‑1 (MQ=255)

                  cTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTa                                                                                                                              <  1:3758167/150‑1 (MQ=255)

                      gCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGa                                                                                                                          >  1:3355941/1‑150 (MQ=255)

                       ccATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGAt                                                                                                                         <  2:1660810/150‑1 (MQ=255)

                       ccATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGAt                                                                                                                         <  2:1716538/150‑1 (MQ=255)

                         aTGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATga                                                                                                                       >  2:3898038/1‑150 (MQ=255)

                           gTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATgaga                                                                                                                     >  2:903308/1‑150 (MQ=255)

                            tttCAGTGAGCGCAGTGTTTTATACGTAACGTCAACTTGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTATGCATAGCAGGTGAGGCAATTGATATTTATTCGCCACTACCCAGTATGGATGAGAt                                                                                                                    <  1:1362635/150‑1 (MQ=255)

                               cAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATNCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCNGTATGGANGAGATCtn                                                                                                                 <  2:2626200/150‑2 (MQ=255)

                                aGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGa                                                                                                                >  2:2337063/1‑150 (MQ=255)

                                 gTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGaa                                                                                                               <  1:3568197/150‑1 (MQ=255)

                                  tGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAATTGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGaaa                                                                                                              >  1:311696/1‑150 (MQ=255)

                                  tGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGaaa                                                                                                              >  2:1121497/1‑150 (MQ=255)

                                  tGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGaaa                                                                                                              >  1:3650678/1‑150 (MQ=255)

                                      cgcAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAgg                                                                                                          <  1:476623/150‑1 (MQ=255)

                                      cgcAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAgg                                                                                                          <  2:2259683/150‑1 (MQ=255)

                                      cgcAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAgg                                                                                                          <  1:230268/150‑1 (MQ=255)

                                      cgcAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAgg                                                                                                          >  2:2545517/1‑150 (MQ=255)

                                      cgcAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAgg                                                                                                          <  2:1189455/150‑1 (MQ=255)

                                       gcAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAggg                                                                                                         <  2:3196929/150‑1 (MQ=255)

                                        cAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGga                                                                                                        >  1:636276/1‑150 (MQ=255)

                                         aGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGgag                                                                                                       <  2:217278/150‑1 (MQ=255)

                                           tgtTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGagag                                                                                                     >  1:1509060/1‑150 (MQ=255)

                                            gtTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAgg                                                                                                    >  2:2892125/1‑150 (MQ=255)

                                             ttttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAggg                                                                                                   <  1:1363806/150‑1 (MQ=255)

                                              tttATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGa                                                                                                  <  2:430846/150‑1 (MQ=255)

                                                tataCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGaaa                                                                                                <  1:2736627/150‑1 (MQ=255)

                                                  taCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATa                                                                                              <  2:1006674/150‑1 (MQ=255)

                                                   aCGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAg                                                                                             >  2:230313/1‑150 (MQ=255)

                                                    cGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGc                                                                                            >  1:2180995/1‑150 (MQ=255)

                                                     gTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGcc                                                                                           <  1:23262/150‑1 (MQ=255)

                                                      tAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGccc                                                                                          <  2:2028135/150‑1 (MQ=255)

                                                      tAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGccc                                                                                          <  2:1068922/150‑1 (MQ=255)

                                                      tAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCATTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGccc                                                                                          <  2:3770006/150‑1 (MQ=255)

                                                         cGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGt                                                                                       >  2:2448272/1‑150 (MQ=255)

                                                         cGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGt                                                                                       <  2:3454198/150‑1 (MQ=255)

                                                          gTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTa                                                                                      >  1:3594369/1‑150 (MQ=255)

                                                            cAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGc                                                                                    >  2:2717317/1‑150 (MQ=255)

                                                              aCATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCt                                                                                  <  1:1495423/150‑1 (MQ=255)

                                                                 tgatgaTCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCa                                                                               >  1:1188202/1‑150 (MQ=255)

                                                                  gatgatCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCAc                                                                              >  1:772872/1‑150 (MQ=255)

                                                                  gatgatCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCAc                                                                              <  1:595108/150‑1 (MQ=255)

                                                                     gatCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTAc                                                                           <  1:944806/150‑1 (MQ=255)

                                                                        ccTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCgg                                                                        <  1:82608/150‑1 (MQ=255)

                                                                        ccTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCgg                                                                        >  1:2200758/1‑150 (MQ=255)

                                                                        ccTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCgg                                                                        >  1:422654/1‑150 (MQ=255)

                                                                         cTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCggg                                                                       >  1:525111/1‑150 (MQ=255)

                                                                         cTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCggg                                                                       <  2:3577360/150‑1 (MQ=255)

                                                                            cGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGgcgc                                                                    >  1:4126483/1‑150 (MQ=255)

                                                                              tAACAACTGCAGCCTTCATGGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAg                                                                  <  1:171792/150‑1 (MQ=255)

                                                                                acaacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGc                                                                >  1:2138908/1‑150 (MQ=255)

                                                                                 caacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCt                                                               <  1:2855328/150‑1 (MQ=255)

                                                                                 caacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCt                                                               >  2:3099444/1‑150 (MQ=255)

                                                                                  aacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCtt                                                              >  2:3575914/1‑150 (MQ=255)

                                                                                  aacTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCtt                                                              >  2:927643/1‑150 (MQ=255)

                                                                                     tGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGa                                                           >  2:2082601/1‑150 (MQ=255)

                                                                                      gCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGAt                                                          <  1:3989651/150‑1 (MQ=255)

                                                                                         gCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGa                                                       >  2:2587766/1‑150 (MQ=255)

                                                                                          ccTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCTAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAg                                                      <  2:780683/150‑1 (MQ=255)

                                                                                               aTAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTAcg                                                 <  2:808971/150‑1 (MQ=255)

                                                                                                 aGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTAcggc                                               >  2:1233819/1‑150 (MQ=255)

                                                                                                  gTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCAGTACCCAGTTTGGATGAGATGTGAAAAAGGGAGAGGGTAATAGGCCGGTATACTTCACTACCGGGCGCAGGCTTAGATGGAGGTAcggcg                                              <  1:3431259/150‑1 (MQ=255)

                                                                                                  gTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTAcggcg                                              <  2:2238431/150‑1 (MQ=255)

                                                                                                        cctTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTc                                        <  1:109976/150‑1 (MQ=255)

                                                                                                            ttCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCgg                                    <  1:1332593/150‑1 (MQ=255)

                                                                                                                gatgatGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAtt                                >  2:1203630/1‑150 (MQ=255)

                                                                                                                gatgatGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTAtt                                >  1:3028606/1‑150 (MQ=255)

                                                                                                                 atgatgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTc                               >  1:1772526/1‑150 (MQ=255)

                                                                                                                 atgatgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTc                               <  1:3688928/150‑1 (MQ=255)

                                                                                                                 atgatgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTc                               >  2:307667/1‑150 (MQ=255)

                                                                                                                   gatgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCgg                             >  2:2380871/1‑150 (MQ=255)

                                                                                                                    atgTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGc                            >  1:1053532/1‑150 (MQ=255)

                                                                                                                           gCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAg                     >  1:1733686/1‑150 (MQ=255)

                                                                                                                            cATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGa                    <  2:4057036/150‑1 (MQ=255)

                                                                                                                              tAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGaaa                  >  1:2682713/1‑150 (MQ=255)

                                                                                                                               aGCAGGTGATGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAt                 <  2:3839871/150‑1 (MQ=255)

                                                                                                                               aGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAt                 <  1:1653228/150‑1 (MQ=255)

                                                                                                                                gCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAtt                <  1:2312119/150‑1 (MQ=255)

                                                                                                                                  aGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAt              >  1:2058252/1‑150 (MQ=255)

                                                                                                                                  aGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAt              <  1:2429485/150‑1 (MQ=255)

                                                                                                                                  aGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAt              >  2:483444/1‑150 (MQ=255)

                                                                                                                                  aGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAt              >  2:1478907/1‑150 (MQ=255)

                                                                                                                                       aGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCa         >  1:3836008/1‑150 (MQ=255)

                                                                                                                                           aaaTGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGAGGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATgg     >  2:3518586/1‑150 (MQ=255)

ACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCT  >  CP009273/1547999‑1548290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTT  >  CP009273/1547999‑1548293

ACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTT                                                                                                                                                   >  SRR19973074.735108/1‑150 (MQ=60)

ACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTT                                                                                                                                                   >  SRR19973074.735119/1‑150 (MQ=60)

ACTCCATTTTCAGCAGGGCTTCGCCATGTCTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTT                                                                                                                                                   >  SRR19973074.2486228/1‑150 (MQ=60)

  TCCATTTTCAGCAGGTCTTCGAAATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACTTGATGATCCTTCGTAACAACTTCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATATCAGGTGAGGCAAATGATATTTAT                                                                                                                                                 <  SRR19973074.4170481/150‑1 (MQ=60)

  TCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTAT                                                                                                                                                 <  SRR19973074.3789990/150‑1 (MQ=60)

  TCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTAT                                                                                                                                                 >  SRR19973074.654146/1‑150 (MQ=60)

   CCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATT                                                                                                                                                <  SRR19973074.3908586/150‑1 (MQ=60)

    CATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTC                                                                                                                                               <  SRR19973074.1361184/150‑1 (MQ=60)

      TTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGC                                                                                                                                             >  SRR19973074.212885/1‑150 (MQ=60)

           AGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTA                                                                                                                                        <  SRR19973074.2350569/150‑1 (MQ=60)

           AGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTA                                                                                                                                        >  SRR19973074.3409384/1‑150 (MQ=60)

           AGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTA                                                                                                                                        >  SRR19973074.2382555/1‑150 (MQ=60)

            GCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTAC                                                                                                                                       <  SRR19973074.1452828/150‑1 (MQ=60)

              AGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCC                                                                                                                                     >  SRR19973074.586749/1‑150 (MQ=60)

               GGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACACA                                                                                                                                    <  SRR19973074.1585794/150‑1 (MQ=60)

                GGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAG                                                                                                                                   <  SRR19973074.2070528/150‑1 (MQ=60)

                GGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAG                                                                                                                                   <  SRR19973074.2070534/150‑1 (MQ=60)

                GGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAG                                                                                                                                   >  SRR19973074.2152968/1‑150 (MQ=60)

                GGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAG                                                                                                                                   <  SRR19973074.1599719/150‑1 (MQ=60)

                  CTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTA                                                                                                                                 <  SRR19973074.3765075/150‑1 (MQ=60)

                      GCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGA                                                                                                                             <  SRR19973074.2252101/150‑1 (MQ=60)

                      GCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGA                                                                                                                             >  SRR19973074.3362167/1‑150 (MQ=60)

                           GTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGA                                                                                                                        <  SRR19973074.126250/150‑1 (MQ=60)

                            TTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACTTGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTATGCATAGCAGGTGAGGCAATTGATATTTATTCGCCACTACCCAGTATGGATGAGAT                                                                                                                       <  SRR19973074.1365159/150‑1 (MQ=60)

                            TTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGAT                                                                                                                       <  SRR19973074.1365210/150‑1 (MQ=60)

                             TTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATC                                                                                                                      >  SRR19973074.1128539/1‑150 (MQ=60)

                               CAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTG                                                                                                                    >  SRR19973074.1704347/1‑150 (MQ=60)

                                 GTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAA                                                                                                                  <  SRR19973074.4071971/150‑1 (MQ=60)

                                 GTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAA                                                                                                                  <  SRR19973074.3574778/150‑1 (MQ=60)

                                 GTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAA                                                                                                                  <  SRR19973074.1754377/150‑1 (MQ=60)

                                  TGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAATTGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAA                                                                                                                 >  SRR19973074.312269/1‑150 (MQ=60)

                                  TGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAA                                                                                                                 >  SRR19973074.3657435/1‑150 (MQ=60)

                                   GAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAA                                                                                                                <  SRR19973074.1788954/150‑1 (MQ=60)

                                      CGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGG                                                                                                             <  SRR19973074.477565/150‑1 (MQ=60)

                                      CGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGG                                                                                                             <  SRR19973074.230768/150‑1 (MQ=60)

                                      CGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGG                                                                                                             <  SRR19973074.230742/150‑1 (MQ=60)

                                        CAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGA                                                                                                           >  SRR19973074.637465/1‑150 (MQ=60)

                                           TGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAG                                                                                                        >  SRR19973074.1512031/1‑150 (MQ=60)

                                           TGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAG                                                                                                        >  SRR19973074.1511906/1‑150 (MQ=60)

                                             TTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGG                                                                                                      <  SRR19973074.1366330/150‑1 (MQ=60)

                                             TTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGG                                                                                                      <  SRR19973074.2617609/150‑1 (MQ=60)

                                               TTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAA                                                                                                    <  SRR19973074.2698005/150‑1 (MQ=60)

                                               TTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAA                                                                                                    >  SRR19973074.2235291/1‑150 (MQ=60)

                                                TATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAA                                                                                                   <  SRR19973074.2741674/150‑1 (MQ=60)

                                                 ATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAAT                                                                                                  <  SRR19973074.3677596/150‑1 (MQ=60)

                                                    CGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGC                                                                                               >  SRR19973074.2185092/1‑150 (MQ=60)

                                                     GTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCC                                                                                              <  SRR19973074.23302/150‑1 (MQ=60)

                                                     GTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCC                                                                                              <  SRR19973074.23325/150‑1 (MQ=60)

                                                     GTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCC                                                                                              <  SRR19973074.1648850/150‑1 (MQ=60)

                                                      TAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCC                                                                                             >  SRR19973074.2997597/1‑150 (MQ=60)

                                                          GTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTA                                                                                         >  SRR19973074.3601008/1‑150 (MQ=60)

                                                              ACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCT                                                                                     <  SRR19973074.1498243/150‑1 (MQ=60)

                                                              ACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCT                                                                                     <  SRR19973074.1311768/150‑1 (MQ=60)

                                                               CATGATGATACTTCGTAACAACTGCAGACTTCATAGTTCCTCCTTTTCGGATGATGTTCTTCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCATTATGGAAGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTT                                                                                    <  SRR19973074.3839039/150‑1 (MQ=60)

                                                                ATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTC                                                                                   >  SRR19973074.503716/1‑150 (MQ=60)

                                                                ATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTC                                                                                   <  SRR19973074.1499646/150‑1 (MQ=60)

                                                                 TGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCA                                                                                  >  SRR19973074.1190469/1‑150 (MQ=60)

                                                                  GATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCAC                                                                                 >  SRR19973074.767634/1‑150 (MQ=60)

                                                                  GATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCAC                                                                                 <  SRR19973074.596250/150‑1 (MQ=60)

                                                                  GATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCAC                                                                                 >  SRR19973074.774357/1‑150 (MQ=60)

                                                                    TGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTA                                                                               <  SRR19973074.1404835/150‑1 (MQ=60)

                                                                     GATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTAC                                                                              <  SRR19973074.946602/150‑1 (MQ=60)

                                                                     GATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTAC                                                                              <  SRR19973074.946679/150‑1 (MQ=60)

                                                                      ATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACC                                                                             <  SRR19973074.2158634/150‑1 (MQ=60)

                                                                        CCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGG                                                                           >  SRR19973074.423470/1‑150 (MQ=60)

                                                                        CCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGG                                                                           <  SRR19973074.868939/150‑1 (MQ=60)

                                                                        CCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGG                                                                           <  SRR19973074.82827/150‑1 (MQ=60)

                                                                        CCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGG                                                                           <  SRR19973074.82809/150‑1 (MQ=60)

                                                                        CCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGG                                                                           >  SRR19973074.423397/1‑150 (MQ=60)

                                                                        CCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGG                                                                           >  SRR19973074.2204899/1‑150 (MQ=60)

                                                                        CCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGG                                                                           >  SRR19973074.2204509/1‑150 (MQ=60)

                                                                         CTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGG                                                                          >  SRR19973074.526158/1‑150 (MQ=60)

                                                                            CGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGC                                                                       >  SRR19973074.4134091/1‑150 (MQ=60)

                                                                             GTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCA                                                                      >  SRR19973074.2438903/1‑150 (MQ=60)

                                                                              TAACAACTGCAGCCTTCATGGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAG                                                                     <  SRR19973074.172167/150‑1 (MQ=60)

                                                                              TAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAG                                                                     <  SRR19973074.172180/150‑1 (MQ=60)

                                                                              TAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAG                                                                     <  SRR19973074.172156/150‑1 (MQ=60)

                                                                                ACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGC                                                                   >  SRR19973074.2142923/1‑150 (MQ=60)

                                                                                 CAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCT                                                                  <  SRR19973074.2860623/150‑1 (MQ=60)

                                                                                 CAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCT                                                                  <  SRR19973074.2860617/150‑1 (MQ=60)

                                                                                      GCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGAT                                                             <  SRR19973074.3997019/150‑1 (MQ=60)

                                                                                           CTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGG                                                        >  SRR19973074.3468057/1‑150 (MQ=60)

                                                                                             TCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTA                                                      <  SRR19973074.3377830/150‑1 (MQ=60)

                                                                                              CATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTAC                                                     <  SRR19973074.471625/150‑1 (MQ=60)

                                                                                                 AGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGC                                                  >  SRR19973074.3057796/1‑150 (MQ=60)

                                                                                                  GTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCAGTACCCAGTTTGGATGAGATGTGAAAAAGGGAGAGGGTAATAGGCCGGTATACTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCG                                                 <  SRR19973074.3437583/150‑1 (MQ=60)

                                                                                                  GTTCCTCCTGTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCG                                                 <  SRR19973074.3587220/150‑1 (MQ=60)

                                                                                                        CCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTC                                           <  SRR19973074.110235/150‑1 (MQ=60)

                                                                                                        CCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTC                                           <  SRR19973074.110242/150‑1 (MQ=60)

                                                                                                        CCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTC                                           <  SRR19973074.2885314/150‑1 (MQ=60)

                                                                                                        CCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCACTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTC                                           <  SRR19973074.2020174/150‑1 (MQ=60)

                                                                                                          TTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGC                                         >  SRR19973074.3688820/1‑150 (MQ=60)

                                                                                                            TTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGG                                       <  SRR19973074.1335085/150‑1 (MQ=60)

                                                                                                              CGGATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTA                                     >  SRR19973074.3423313/1‑150 (MQ=60)

                                                                                                                GATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATT                                   >  SRR19973074.3034205/1‑150 (MQ=60)

                                                                                                                 ATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTC                                  <  SRR19973074.3695749/150‑1 (MQ=60)

                                                                                                                 ATGATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTC                                  >  SRR19973074.1775856/1‑150 (MQ=60)

                                                                                                                   GATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGG                                >  SRR19973074.1481926/1‑150 (MQ=60)

                                                                                                                    ATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGC                               >  SRR19973074.1055472/1‑150 (MQ=60)

                                                                                                                    ATGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGC                               >  SRR19973074.1371837/1‑150 (MQ=60)

                                                                                                                     TGTTCTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCT                              <  SRR19973074.3326750/150‑1 (MQ=60)

                                                                                                                         CTGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCC                          >  SRR19973074.3353299/1‑150 (MQ=60)

                                                                                                                          TGCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCA                         <  SRR19973074.3619893/150‑1 (MQ=60)

                                                                                                                           GCATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAG                        >  SRR19973074.1736894/1‑150 (MQ=60)

                                                                                                                            CATAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGA                       >  SRR19973074.3715250/1‑150 (MQ=60)

                                                                                                                              TAGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAA                     >  SRR19973074.2687698/1‑150 (MQ=60)

                                                                                                                               AGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAT                    >  SRR19973074.597989/1‑150 (MQ=60)

                                                                                                                               AGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAT                    <  SRR19973074.1656350/150‑1 (MQ=60)

                                                                                                                               AGCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAAT                    <  SRR19973074.2618693/150‑1 (MQ=60)

                                                                                                                                GCAGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATT                   <  SRR19973074.2316442/150‑1 (MQ=60)

                                                                                                                                  AGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAT                 >  SRR19973074.2062118/1‑150 (MQ=60)

                                                                                                                                  AGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAT                 <  SRR19973074.2433920/150‑1 (MQ=60)

                                                                                                                                  AGGTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTAT                 <  SRR19973074.2433979/150‑1 (MQ=60)

                                                                                                                                    GTGAGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCG               >  SRR19973074.2337154/1‑150 (MQ=60)

                                                                                                                                       AGGCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCA            >  SRR19973074.3843000/1‑150 (MQ=60)

                                                                                                                                         GCAAATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAAT          >  SRR19973074.576248/1‑150 (MQ=60)

                                                                                                                                            AATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGC       >  SRR19973074.794354/1‑150 (MQ=60)

                                                                                                                                             ATGATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCC      <  SRR19973074.3554405/150‑1 (MQ=60)

                                                                                                                                               GATATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTG    <  SRR19973074.3854414/150‑1 (MQ=60)

                                                                                                                                                 TATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTT  <  SRR19973074.1331663/150‑1 (MQ=60)

ACTCCATTTTCAGCAGGGCTTCGCCATGTTTCAGTGAGCGCAGTGTTTTATACGTAACGTCAACATGATGATCCTTCGTAACAACTGCAGCCTTCATAGTTCCTCCTTTTCGGATGATGTTCTGCATAGCAGGTGAGGCAAATGAGATTTATTCGCCACTACCCAGTATGGATGAGATCTGAAAAAGGGAGAGGGAAATAGCCCGGTAGCCTTCACTACCGGGCGCAGGCTTAGATGGAGGTACGGCGGTAGTCGCGGTATTCGGCTTGCCAGAAATTATCGTCAATGGCCTGTT  >  CP009273/1547999‑1548293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`