Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,292,271 A→T 18.1% C263S (TGC→AGC)  napH ← ferredoxin‑type protein essential for electron transfer from ubiquinol to periplasmic nitrate reductase (NapAB)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,292,2710AT18.1% 680.9 / 137.4 253C263S (TGC→AGC) napHferredoxin‑type protein essential for electron transfer from ubiquinol to periplasmic nitrate reductase (NapAB)
Reads supporting (aligned to +/- strand):  ref base A (108/99);  new base T (25/21);  total (133/120)
Fisher's exact test for biased strand distribution p-value = 8.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

AACCGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTAATCACACCTT  >  CP009273/2292123‑2292420
                                                                                                                                                    |                                                                                                                                                     
 aCCGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCATAACAGACATCCACGCAGCGACCGCACg                                                                                                                                                     <  1:659811/150‑1 (MQ=255)
     cgccgcTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCAt                                                                                                                                                 <  2:3964998/150‑1 (MQ=255)
           ttACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATc                                                                                                                                           >  2:4485687/1‑150 (MQ=255)
           ttACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATc                                                                                                                                           <  2:1700729/150‑1 (MQ=255)
           ttACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATc                                                                                                                                           >  1:574633/1‑150 (MQ=255)
            tACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATcg                                                                                                                                          >  2:4503823/1‑150 (MQ=255)
              ccaccaGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATcgcg                                                                                                                                        >  1:2613736/1‑150 (MQ=255)
                accaGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGc                                                                                                                                      >  1:3774201/1‑150 (MQ=255)
                   aGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTgg                                                                                                                                   >  1:568042/1‑150 (MQ=255)
                      gCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGa                                                                                                                                >  2:3170203/1‑150 (MQ=255)
                         aGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCt                                                                                                                             >  2:4449470/1‑150 (MQ=255)
                          gCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCTAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTg                                                                                                                            <  2:4332190/150‑1 (MQ=255)
                            aTCGCCGTCCATTGACACAGCGCTGTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCa                                                                                                                          <  2:3305973/150‑1 (MQ=255)
                              cGCCGGCCATTGACACAGCGCTTTCTTCCGGTCTTGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTTTTGTAAATACATCCACAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCAcc                                                                                                                        <  1:3424175/150‑1 (MQ=255)
                                  gTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGc                                                                                                                    >  2:4160874/1‑150 (MQ=255)
                                   tCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCt                                                                                                                   <  1:4066088/150‑1 (MQ=255)
                                    ccATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCtt                                                                                                                  <  1:3246200/150‑1 (MQ=255)
                                     cATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCttt                                                                                                                 >  1:3083286/1‑150 (MQ=255)
                                      aTTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTg                                                                                                                <  2:7297/150‑1 (MQ=255)
                                           cacaGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCACTCGCGGCTGGTGACCTGCACCGGGCTTTGTTCAt                                                                                                           >  2:3209606/1‑150 (MQ=255)
                                           cacaGCGCTTGCTTCAGGTCATGGCTTTTCATGATGTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCAt                                                                                                           >  2:1448128/1‑150 (MQ=255)
                                             caGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATcc                                                                                                         >  1:1786546/1‑150 (MQ=255)
                                             caGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGATTTGTTCATcc                                                                                                         >  1:955663/1‑150 (MQ=255)
                                              aGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCa                                                                                                        >  1:4531697/1‑150 (MQ=255)
                                                      cttcAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCggg                                                                                                >  1:4324120/1‑150 (MQ=255)
                                                      cttcAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCggg                                                                                                <  2:2461290/150‑1 (MQ=255)
                                                       ttcAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCgggg                                                                                               <  2:1838207/150‑1 (MQ=255)
                                                         cAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCa                                                                                             >  1:1559514/1‑150 (MQ=255)
                                                              cATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAg                                                                                        >  2:658709/1‑150 (MQ=255)
                                                              cATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAg                                                                                        >  2:2548914/1‑150 (MQ=255)
                                                                  gCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACAGGGGCACGTAGcaca                                                                                    >  1:1935686/1‑150 (MQ=255)
                                                                     tttCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGc                                                                                 <  1:2798095/150‑1 (MQ=255)
                                                                     tttCATGATGTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAAGACATCCTCAGAACAGACATCCACGCCGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGc                                                                                 <  2:4532765/150‑1 (MQ=255)
                                                                      ttCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCg                                                                                >  1:2837596/1‑150 (MQ=255)
                                                                        cATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGt                                                                              >  2:1023003/1‑150 (MQ=255)
                                                                        cATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGt                                                                              >  2:2180261/1‑150 (MQ=255)
                                                                         aTGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGtt                                                                             >  1:585998/1‑150 (MQ=255)
                                                                          tGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTc                                                                            >  1:1696545/1‑150 (MQ=255)
                                                                           gATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTcc                                                                           >  2:1762711/1‑150 (MQ=255)
                                                                           gATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTcc                                                                           <  1:4327740/150‑1 (MQ=255)
                                                                            aTTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCg                                                                          <  1:1875058/150‑1 (MQ=255)
                                                                              ttCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCggg                                                                        <  2:2348873/150‑1 (MQ=255)
                                                                              ttCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCggg                                                                        >  1:997731/1‑150 (MQ=255)
                                                                                ngCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCa                                                                      >  2:2200746/2‑150 (MQ=255)
                                                                                cGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCa                                                                      <  1:3948344/150‑1 (MQ=255)
                                                                                 gCTCCCGAACTCCATCGTTTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCaa                                                                     <  1:2579878/150‑1 (MQ=255)
                                                                                 gCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCaa                                                                     <  1:4406441/150‑1 (MQ=255)
                                                                                    cccGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACa                                                                  >  2:2863950/1‑150 (MQ=255)
                                                                                     ccGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACAt                                                                 >  1:1093765/1‑150 (MQ=255)
                                                                                      cGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATg                                                                >  2:559086/1‑150 (MQ=255)
                                                                                      cGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATg                                                                <  1:863482/150‑1 (MQ=255)
                                                                                       gAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGa                                                               <  2:3452034/150‑1 (MQ=255)
                                                                                       gAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGa                                                               <  2:3452609/150‑1 (MQ=255)
                                                                                        aaCTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGaa                                                              <  1:3623093/150‑1 (MQ=255)
                                                                                             cATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACaa                                                         >  2:2617859/1‑150 (MQ=255)
                                                                                             cATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACaa                                                         >  1:3490695/1‑150 (MQ=255)
                                                                                                cGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATcc                                                      <  1:2479743/150‑1 (MQ=255)
                                                                                                  tgtAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCAt                                                    >  1:95717/1‑150 (MQ=255)
                                                                                                  tgtAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCAt                                                    >  1:1252704/1‑150 (MQ=255)
                                                                                                   gtAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATa                                                   <  1:1199569/150‑1 (MQ=255)
                                                                                                    tAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATAc                                                  <  2:78952/150‑1 (MQ=255)
                                                                                                     aGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACa                                                 >  2:317491/1‑150 (MQ=255)
                                                                                                      gTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAg                                                <  2:4062213/150‑1 (MQ=255)
                                                                                                       ttATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGc                                               <  2:97273/150‑1 (MQ=255)
                                                                                                       ttATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGc                                               <  2:2437837/150‑1 (MQ=255)
                                                                                                        tATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCg                                              <  1:4044145/150‑1 (MQ=255)
                                                                                                          ttGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGt                                            >  2:2906077/1‑150 (MQ=255)
                                                                                                          ttGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGt                                            <  1:3346671/150‑1 (MQ=255)
                                                                                                           tGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGtt                                           <  1:4091597/150‑1 (MQ=255)
                                                                                                           tGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGtt                                           <  2:2566227/150‑1 (MQ=255)
                                                                                                                aTACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACAtt                                      <  2:2044641/150‑1 (MQ=255)
                                                                                                                 tACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACAttt                                     <  2:950571/150‑1 (MQ=255)
                                                                                                                 tACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACAttt                                     >  2:1246671/1‑150 (MQ=255)
                                                                                                                  aCATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTc                                    <  2:3940533/150‑1 (MQ=255)
                                                                                                                   cATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCt                                   <  2:3337531/150‑1 (MQ=255)
                                                                                                                    aTCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTg                                  <  1:4476882/150‑1 (MQ=255)
                                                                                                                      ccTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGcc                                <  2:1351981/150‑1 (MQ=255)
                                                                                                                        tCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGa                              >  1:4162247/1‑150 (MQ=255)
                                                                                                                         cAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGAt                             <  2:2612989/150‑1 (MQ=255)
                                                                                                                          aGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATc                            >  1:828641/1‑150 (MQ=255)
                                                                                                                          aGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATc                            <  2:3032466/150‑1 (MQ=255)
                                                                                                                           gAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCg                           <  2:1995649/150‑1 (MQ=255)
                                                                                                                           gAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCg                           >  1:1833583/1‑150 (MQ=255)
                                                                                                                            aaCAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCCCGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCgg                          >  2:3739215/1‑150 (MQ=255)
                                                                                                                            aaCAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCgg                          >  1:1365730/1‑150 (MQ=255)
                                                                                                                            aaCAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCgg                          <  2:2717857/150‑1 (MQ=255)
                                                                                                                             aCAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGt                         >  1:4182878/1‑150 (MQ=255)
                                                                                                                              cAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTTGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTg                        <  2:3537179/150‑1 (MQ=255)
                                                                                                                                 aCATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGcc                     >  2:35692/1‑150 (MQ=255)
                                                                                                                                  cATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCg                    <  2:1649615/150‑1 (MQ=255)
                                                                                                                                  cATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGGGGCCg                    <  1:2075528/150‑1 (MQ=255)
                                                                                                                                        cGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCACCgg              >  2:1191876/1‑150 (MQ=255)
                                                                                                                                          cAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAGCATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTa            <  1:3978327/150‑1 (MQ=255)
                                                                                                                                             cGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTAATc         >  2:4119816/1‑150 (MQ=255)
                                                                                                                                             cGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTAATc         >  1:2033133/1‑150 (MQ=255)
                                                                                                                                               aCCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTAATcac       >  1:2499821/1‑150 (MQ=255)
                                                                                                                                                ccGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTAATcaca      <  1:1143549/150‑1 (MQ=255)
                                                                                                                                                 cGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTAATCacac     >  2:4256985/1‑150 (MQ=255)
                                                                                                                                                  gCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTAATCACAcc    <  1:2706155/150‑1 (MQ=255)
                                                                                                                                                    aCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTAATCACACCtt  <  2:110777/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AACCGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTAATCACACCTT  >  CP009273/2292123‑2292420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A