Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,292,271 A→T 18.6% C263S (TGC→AGC)  napH ← ferredoxin‑type protein essential for electron transfer from ubiquinol to periplasmic nitrate reductase (NapAB)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,292,2710AT18.6% 697.2 / 155.4 279C263S (TGC→AGC) napHferredoxin‑type protein essential for electron transfer from ubiquinol to periplasmic nitrate reductase (NapAB)
Reads supporting (aligned to +/- strand):  ref base A (124/103);  new base T (25/27);  total (149/130)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.76e-01

Only 100 of 281 total aligned reads displayed.

AAACCGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTAATCACAC  >  CP009273/2292122‑2292417
                                                                                                                                                     |                                                                                                                                                  
aaaCCGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCa                                                                                                                                                    >  2:4459567/1‑150 (MQ=255)
  aCCGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACg                                                                                                                                                  <  1:85465/150‑1 (MQ=255)
   ccGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGt                                                                                                                                                 <  1:1067940/150‑1 (MQ=255)
    cgcgCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTc                                                                                                                                                >  2:2759240/1‑150 (MQ=255)
       gccgcTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATg                                                                                                                                             >  1:586996/1‑150 (MQ=255)
       gccgcTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATg                                                                                                                                             <  2:2652851/150‑1 (MQ=255)
           cTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAAt                                                                                                                                         <  1:4248585/150‑1 (MQ=255)
           cTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAAt                                                                                                                                         <  2:558386/150‑1 (MQ=255)
              accaccAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATcgc                                                                                                                                      <  1:2084980/150‑1 (MQ=255)
              accaccAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATcgc                                                                                                                                      >  2:2901206/1‑150 (MQ=255)
               ccaccaGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCCGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATcgcg                                                                                                                                     <  2:3906404/150‑1 (MQ=255)
               ccaccaGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATcgcg                                                                                                                                     >  2:2175101/1‑150 (MQ=255)
                      ggCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTg                                                                                                                              <  1:2753497/150‑1 (MQ=255)
                          aGCATCGCCGTCCAATGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCt                                                                                                                          <  2:3595407/150‑1 (MQ=255)
                           gCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATTATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTg                                                                                                                         <  1:2780589/150‑1 (MQ=255)
                               cGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCAcc                                                                                                                     <  2:3420892/150‑1 (MQ=255)
                               cGCCGTCCATTGACACAGCGCTGTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCAcc                                                                                                                     <  2:3270047/150‑1 (MQ=255)
                                gCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCg                                                                                                                    >  2:3955813/1‑150 (MQ=255)
                                   gTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGc                                                                                                                 >  2:1024891/1‑150 (MQ=255)
                                     ccATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCtt                                                                                                               <  1:4350809/150‑1 (MQ=255)
                                       aTTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTg                                                                                                             >  2:68772/1‑150 (MQ=255)
                                            cacaGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCAt                                                                                                        <  1:1150297/150‑1 (MQ=255)
                                             acaGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATc                                                                                                       >  2:1578264/1‑150 (MQ=255)
                                              caGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATcc                                                                                                      >  2:792015/1‑150 (MQ=255)
                                               aGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGTCATCCAAGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCa                                                                                                     >  2:4838560/1‑150 (MQ=255)
                                               aGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCa                                                                                                     <  2:1694279/150‑1 (MQ=255)
                                               aGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCa                                                                                                     <  1:458019/150‑1 (MQ=255)
                                                gcgcTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAg                                                                                                    >  2:1781636/1‑150 (MQ=255)
                                                 cgcTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGc                                                                                                   <  2:1049813/150‑1 (MQ=255)
                                                 cgcTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGc                                                                                                   <  1:3810560/150‑1 (MQ=255)
                                                     ttcttcAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCg                                                                                               >  1:3314756/1‑150 (MQ=255)
                                                       cttcAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCggg                                                                                             >  1:1061759/1‑150 (MQ=255)
                                                       cttcAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCggg                                                                                             <  1:1977211/150‑1 (MQ=255)
                                                            ggTCATGGCTTTTCATGATGTCGCTCCCGAACTCCATCGTGTAGTTATTGTACAGACCTCCTCCGACCCGACCTCCACGCAGCGACCGCACGTCATGCACTCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACg                                                                                        <  2:84425/150‑1 (MQ=255)
                                                               cATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAg                                                                                     >  1:5079453/1‑150 (MQ=255)
                                                               cATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAg                                                                                     >  1:313527/1‑150 (MQ=255)
                                                                aTGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGc                                                                                    >  2:1479590/1‑150 (MQ=255)
                                                                aTGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGc                                                                                    >  1:1924699/1‑150 (MQ=255)
                                                                  ggCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGcac                                                                                  >  1:538771/1‑150 (MQ=255)
                                                                    cTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACAt                                                                                >  1:4473252/1‑150 (MQ=255)
                                                                      tttCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGc                                                                              <  1:3688879/150‑1 (MQ=255)
                                                                      tttCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGc                                                                              >  1:4230697/1‑150 (MQ=255)
                                                                       ttCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCg                                                                             <  2:3072554/150‑1 (MQ=255)
                                                                          aTGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGtt                                                                          >  2:5039634/1‑150 (MQ=255)
                                                                            gATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTcc                                                                        <  2:5032912/150‑1 (MQ=255)
                                                                            gATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTcc                                                                        >  2:849209/1‑150 (MQ=255)
                                                                            gATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTcc                                                                        <  2:587059/150‑1 (MQ=255)
                                                                            gATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTcc                                                                        <  2:1362072/150‑1 (MQ=255)
                                                                             aTTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCg                                                                       >  1:501023/1‑150 (MQ=255)
                                                                                tCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGc                                                                    >  2:1306443/1‑150 (MQ=255)
                                                                                 cGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCa                                                                   <  2:3735875/150‑1 (MQ=255)
                                                                                 cGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCa                                                                   >  2:1873627/1‑150 (MQ=255)
                                                                                    tCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAAc                                                                >  2:1295782/1‑150 (MQ=255)
                                                                                        gAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGa                                                            >  2:5118326/1‑150 (MQ=255)
                                                                                        gAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGa                                                            >  2:2335496/1‑150 (MQ=255)
                                                                                        gAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGa                                                            >  2:3043931/1‑150 (MQ=255)
                                                                                        gAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGa                                                            >  1:3920633/1‑150 (MQ=255)
                                                                                        gAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGa                                                            <  1:1262561/150‑1 (MQ=255)
                                                                                         aaCTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGaa                                                           <  2:2325955/150‑1 (MQ=255)
                                                                                         aaCTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGaa                                                           >  1:686394/1‑150 (MQ=255)
                                                                                            tCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAAc                                                        >  2:4262097/1‑150 (MQ=255)
                                                                                             ccATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACa                                                       >  1:3330838/1‑150 (MQ=255)
                                                                                              cATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACaa                                                      <  2:1492334/150‑1 (MQ=255)
                                                                                               aTCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAAt                                                     >  1:2596077/1‑150 (MQ=255)
                                                                                               aTCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAAt                                                     >  2:2795006/1‑150 (MQ=255)
                                                                                                tCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATc                                                    <  1:234124/150‑1 (MQ=255)
                                                                                                 cGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATcc                                                   <  1:3415020/150‑1 (MQ=255)
                                                                                                 cGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATcc                                                   >  1:2176859/1‑150 (MQ=255)
                                                                                                  gtgtAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCa                                                  <  2:1445857/150‑1 (MQ=255)
                                                                                                  gtgtAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCa                                                  <  1:4429465/150‑1 (MQ=255)
                                                                                                   tgtAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCAt                                                 >  1:16466/1‑150 (MQ=255)
                                                                                                     tAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATAc                                               >  1:946267/1‑150 (MQ=255)
                                                                                                         tATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACATGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCg                                           >  2:467976/1‑150 (MQ=255)
                                                                                                          aTTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCgg                                          <  2:4378996/150‑1 (MQ=255)
                                                                                                          aTTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCgg                                          >  2:3656235/1‑150 (MQ=255)
                                                                                                             gTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTa                                       <  2:4980396/150‑1 (MQ=255)
                                                                                                             gTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTa                                       >  1:485064/1‑150 (MQ=255)
                                                                                                               aaaTACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACa                                     <  1:3482111/150‑1 (MQ=255)
                                                                                                                aaTACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACAt                                    >  1:898184/1‑150 (MQ=255)
                                                                                                                    cATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCt                                <  2:188044/150‑1 (MQ=255)
                                                                                                                     aTCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTg                               <  1:3938022/150‑1 (MQ=255)
                                                                                                                       ccTCAGAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGcc                             >  2:1494806/1‑150 (MQ=255)
                                                                                                                       ccTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGcc                             >  2:4183778/1‑150 (MQ=255)
                                                                                                                            gAACAGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCg                        <  2:5003241/150‑1 (MQ=255)
                                                                                                                            gAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCg                        <  2:1978484/150‑1 (MQ=255)
                                                                                                                            gAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCg                        <  1:152758/150‑1 (MQ=255)
                                                                                                                             aaCAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCgg                       >  1:2710290/1‑150 (MQ=255)
                                                                                                                             aaCAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCgg                       <  1:1465969/150‑1 (MQ=255)
                                                                                                                                aGACATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTgg                    >  1:159505/1‑150 (MQ=255)
                                                                                                                                  aCATCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGcc                  >  2:954796/1‑150 (MQ=255)
                                                                                                                                  aCATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGcc                  <  2:3180276/150‑1 (MQ=255)
                                                                                                                                    aTCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGc                <  2:2518323/150‑1 (MQ=255)
                                                                                                                                     tCCACGCAGCGACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCa               >  1:2560241/1‑150 (MQ=255)
                                                                                                                                     tCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCa               <  2:2287513/150‑1 (MQ=255)
                                                                                                                                     tCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCa               >  2:2405464/1‑150 (MQ=255)
                                                                                                                                          gCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGt          >  1:4062157/1‑150 (MQ=255)
                                                                                                                                           cAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTa         >  1:3827991/1‑150 (MQ=255)
                                                                                                                                               gACCGCTCGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTAATca     <  1:822720/150‑1 (MQ=255)
                                                                                                                                               gACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTAATca     <  2:3801986/150‑1 (MQ=255)
                                                                                                                                                  cGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTAATCacac  <  2:2096780/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AAACCGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACAGACATCCACGCAGCGACCGCACGTCATGCAATCGCGGCTGGTGACCTGCACCGGGCTTTGTTCATCCAGCACCGGGGCACGTAGCACATGCGGTTCCGGGCAAACATGAAAACAATCCATACAGCGGTTACATTTCTGCCGATCGGTGGCCGCAACGGTAATCACAC  >  CP009273/2292122‑2292417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A