Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F121 I0 R1	856	273.6	9081716	94.1%	8545894	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,785,915	G→T	85.3%	intergenic (‑4/+271)	kbl ← / ← yibB	glycine C‑acetyltransferase/YibB family protein, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,785,915	0	G	T	85.3%	591.4 / 106.1	232	intergenic (‑4/+271)	kbl/yibB	glycine C‑acetyltransferase/YibB family protein, function unknown
Reads supporting (aligned to +/- strand): ref base G (13/21); new base T (89/109); total (102/130)
Fisher's exact test for biased strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 233 total aligned reads displayed.

ACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGT  >  CP009273/3785768‑3786064

acaAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTAt                                                                                                                                                     >  2:1022260/1‑150 (MQ=255)

 caAAAGTTAATGACGTGGTTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTAtt                                                                                                                                                    <  1:784836/150‑1 (MQ=255)

  aaaaGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATtc                                                                                                                                                   <  1:3584536/150‑1 (MQ=255)

  aaaaGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATtc                                                                                                                                                   <  1:3584600/150‑1 (MQ=255)

     aGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTcc                                                                                                                                                <  1:652746/150‑1 (MQ=255)

     aGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTcc                                                                                                                                                <  2:3937009/150‑1 (MQ=255)

     aGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTcc                                                                                                                                                <  2:437267/150‑1 (MQ=255)

     aGTTAAGGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTcc                                                                                                                                                <  2:1655289/150‑1 (MQ=255)

      gTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCa                                                                                                                                               >  2:1157055/1‑150 (MQ=255)

      gTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCa                                                                                                                                               >  2:2027105/1‑150 (MQ=255)

      gTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCa                                                                                                                                               >  2:3828223/1‑150 (MQ=255)

         aaTGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGAc                                                                                                                                            >  2:3193590/1‑150 (MQ=255)

             aCGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAggg                                                                                                                                        <  1:4260815/150‑1 (MQ=255)

             aCGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGAGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCATATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAggg                                                                                                                                        >  1:9652/1‑150 (MQ=255)

               gTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCa                                                                                                                                      <  2:945614/150‑1 (MQ=255)

                    ttCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTc                                                                                                                                 >  1:959357/1‑150 (MQ=255)

                    ttCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTc                                                                                                                                 <  1:455372/150‑1 (MQ=255)

                         tCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGca                                                                                                                            >  1:538529/1‑150 (MQ=255)

                         tCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGca                                                                                                                            >  2:752272/1‑150 (MQ=255)

                              cacaGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACatat                                                                                                                       <  2:2210980/150‑1 (MQ=255)

                                caGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTa                                                                                                                     <  1:996974/150‑1 (MQ=255)

                                caGTGATATCTGCTTGCTGCGCAGACGTAATAAGGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTa                                                                                                                     <  1:1000326/150‑1 (MQ=255)

                                       atCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAAc                                                                                                              <  2:207518/150‑1 (MQ=255)

                                         cTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACtt                                                                                                            <  2:1109745/150‑1 (MQ=255)

                                         cTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACtt                                                                                                            <  1:3147268/150‑1 (MQ=255)

                                          tgcttgctGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTta                                                                                                           >  1:3257160/1‑150 (MQ=255)

                                            cttgctGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTtata                                                                                                         >  1:2894419/1‑150 (MQ=255)

                                             ttgctGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAg                                                                                                        >  1:918885/1‑150 (MQ=255)

                                              tgctgcGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAgg                                                                                                       >  1:165120/1‑150 (MQ=255)

                                              tgctgcGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAgg                                                                                                       <  1:2485549/150‑1 (MQ=255)

                                              tgctgcGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAgg                                                                                                       <  1:1173652/150‑1 (MQ=255)

                                                ctgcGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTGACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTc                                                                                                     <  2:1574297/150‑1 (MQ=255)

                                                ctgcGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACTGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTc                                                                                                     <  2:1459690/150‑1 (MQ=255)

                                                  gcgcAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTcgc                                                                                                   <  1:314072/150‑1 (MQ=255)

                                                   cgcAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTcgcg                                                                                                  >  2:4476670/1‑150 (MQ=255)

                                                   cgcAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTcgcg                                                                                                  <  1:3790940/150‑1 (MQ=255)

                                                   cgcAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTcgcg                                                                                                  <  1:3791045/150‑1 (MQ=255)

                                                     cAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGcgac                                                                                                <  2:347739/150‑1 (MQ=255)

                                                        aCGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACgag                                                                                             >  1:3040236/1‑150 (MQ=255)

                                                         cGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACgaga                                                                                            <  2:1694086/150‑1 (MQ=255)

                                                         cGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACgaga                                                                                            <  1:4344477/150‑1 (MQ=255)

                                                            aataatGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATaa                                                                                         >  1:3852835/1‑150 (MQ=255)

                                                              taatGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAAcg                                                                                       <  2:491029/150‑1 (MQ=255)

                                                              taatGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAAcg                                                                                       <  1:2645038/150‑1 (MQ=255)

                                                                   cgcTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTa                                                                                  >  2:269843/1‑150 (MQ=255)

                                                                      tcttctTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAaca                                                                               <  2:528064/150‑1 (MQ=255)

                                                                       cttcatTAAACACCCCGTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAacac                                                                              <  2:2422523/150‑1 (MQ=255)

                                                                         tcttTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACtt                                                                            <  2:1409484/150‑1 (MQ=255)

                                                                           tttAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCt                                                                          <  1:1597006/150‑1 (MQ=255)

                                                                           tttAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCt                                                                          <  1:2458008/150‑1 (MQ=255)

                                                                            ttAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTg                                                                         >  1:878565/1‑150 (MQ=255)

                                                                             tAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGc                                                                        >  2:1431727/1‑150 (MQ=255)

                                                                               aacaacCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGTTCGCGACGAGATAACGCGTTAACACTTCTGCaa                                                                      >  2:1856311/1‑150 (MQ=255)

                                                                                  aacCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAAt                                                                   <  2:3012815/150‑1 (MQ=255)

                                                                                   acCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAAtt                                                                  >  2:890039/1‑150 (MQ=255)

                                                                                   acCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAAtt                                                                  <  2:3357398/150‑1 (MQ=255)

                                                                                   acCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAAtt                                                                  <  1:2183735/150‑1 (MQ=255)

                                                                                   acCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAAtt                                                                  <  1:4397899/150‑1 (MQ=255)

                                                                                   acCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAAtt                                                                  <  2:304418/150‑1 (MQ=255)

                                                                                   acCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAAtc                                                                  <  1:2183563/150‑2 (MQ=255)

                                                                                     ccTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCa                                                                <  2:3558217/150‑1 (MQ=255)

                                                                                           gCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGataa                                                          >  1:393100/1‑150 (MQ=255)

                                                                                            cccGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGataaa                                                         <  1:3232583/150‑1 (MQ=255)

                                                                                            cccGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGataaa                                                         <  1:2513457/150‑1 (MQ=255)

                                                                                            cccGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGataaa                                                         <  1:3232520/150‑1 (MQ=255)

                                                                                            cccGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGataaa                                                         >  2:2256794/1‑150 (MQ=255)

                                                                                              cGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGataaatc                                                       >  1:394155/1‑149 (MQ=255)

                                                                                              cGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGataaata                                                       <  1:610995/150‑1 (MQ=255)

                                                                                              cGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGataaata                                                       >  1:394021/1‑150 (MQ=255)

                                                                                               gTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGataaataa                                                      >  2:3777919/1‑150 (MQ=255)

                                                                                                tGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAAc                                                     <  1:242364/150‑1 (MQ=255)

                                                                                                   ggTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACgtg                                                  >  1:1838369/1‑150 (MQ=255)

                                                                                                    gTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACgtgt                                                 >  2:3945687/1‑150 (MQ=255)

                                                                                                        ccAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACt                                             <  1:10038/150‑1 (MQ=255)

                                                                                                            aTCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGAt                                         <  1:2849549/150‑1 (MQ=255)

                                                                                                              cGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGt                                       >  2:2773625/1‑150 (MQ=255)

                                                                                                                ttGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGtct                                     <  1:1378527/150‑1 (MQ=255)

                                                                                                                 tGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGtctc                                    >  2:4159624/1‑150 (MQ=255)

                                                                                                                 tGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGtctc                                    >  1:4008104/1‑150 (MQ=255)

                                                                                                                 tGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAAGATAAATAACGTGTAACTTGATGtctc                                    >  2:4045301/1‑150 (MQ=255)

                                                                                                                  ggTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCTGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGtctct                                   >  1:3157257/1‑150 (MQ=255)

                                                                                                                  ggTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGtctct                                   >  1:3036076/1‑150 (MQ=255)

                                                                                                                   gTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGtctctc                                  >  1:1786512/1‑150 (MQ=255)

                                                                                                                   gTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGtctctc                                  <  1:739275/150‑1 (MQ=255)

                                                                                                                     tAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCaa                                >  2:2978209/1‑150 (MQ=255)

                                                                                                                     tAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCAAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCaa                                >  2:4356264/1‑150 (MQ=255)

                                                                                                                           ctgATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTa                          <  1:3895532/150‑1 (MQ=255)

                                                                                                                             gATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACa                        >  2:492939/1‑150 (MQ=255)

                                                                                                                             gATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGAGAAATAACGTGTAACTTGATGTCTCTCAACACCTACa                        >  1:4460149/1‑150 (MQ=255)

                                                                                                                                aaaaTTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGAt                     >  1:3364676/1‑150 (MQ=255)

                                                                                                                                aaaaTTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGAt                     <  1:2579825/150‑1 (MQ=255)

                                                                                                                                  aaTTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGAtag                   <  2:1281546/150‑1 (MQ=255)

                                                                                                                                  aaTTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGAtag                   <  1:3827850/150‑1 (MQ=255)

                                                                                                                                  aaTTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCAGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGAtag                   <  2:1281782/150‑1 (MQ=255)

                                                                                                                                    ttCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGAtagta                 >  1:1989200/1‑150 (MQ=255)

                                                                                                                                      ctcCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGa               <  1:492939/150‑1 (MQ=255)

                                                                                                                                         cACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGaaaa            >  1:2724066/1‑150 (MQ=255)

                                                                                                                                            gCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACt         <  1:1022260/150‑1 (MQ=255)

                                                                                                                                              aTTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGt       >  1:1776341/1‑150 (MQ=255)

                                                                                                                                                tGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTAc     <  1:3089936/150‑1 (MQ=255)

ACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGT  >  CP009273/3785768‑3786064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGT  >  CP009273/3785768‑3786064

ACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTAT                                                                                                                                                     >  SRR19973080.1304810/1‑150 (MQ=60)

 CAAAAGTTAATGACGTGGTTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATT                                                                                                                                                    <  SRR19973080.789348/150‑1 (MQ=60)

 CAAAAGTTAATGACGTGGTTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATT                                                                                                                                                    <  SRR19973080.787375/150‑1 (MQ=60)

  AAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTC                                                                                                                                                   <  SRR19973080.3604422/150‑1 (MQ=60)

  AAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTC                                                                                                                                                   <  SRR19973080.3604378/150‑1 (MQ=60)

  AAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTC                                                                                                                                                   <  SRR19973080.3604357/150‑1 (MQ=60)

     AGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCC                                                                                                                                                <  SRR19973080.656706/150‑1 (MQ=60)

     AGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCC                                                                                                                                                <  SRR19973080.656684/150‑1 (MQ=60)

      GTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCA                                                                                                                                               <  SRR19973080.2499411/150‑1 (MQ=60)

      GTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGAGTCTCCA                                                                                                                                               <  SRR19973080.2754620/150‑1 (MQ=60)

       TTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAG                                                                                                                                              <  SRR19973080.681714/150‑1 (MQ=60)

           TGACGGGGCGTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTACTACTGCGCTCTTCTTTAAACACCCCGTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAG                                                                                                                                          <  SRR19973080.4394629/150‑1 (MQ=60)

             ACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGG                                                                                                                                        <  SRR19973080.2946860/150‑1 (MQ=60)

             ACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGG                                                                                                                                        <  SRR19973080.4284061/150‑1 (MQ=60)

             ACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGG                                                                                                                                        >  SRR19973080.2288833/1‑150 (MQ=60)

             ACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGG                                                                                                                                        >  SRR19973080.9835/1‑150 (MQ=60)

             ACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGG                                                                                                                                        >  SRR19973080.3610964/1‑150 (MQ=60)

             ACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGAGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCATATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGG                                                                                                                                        >  SRR19973080.9760/1‑150 (MQ=60)

              CGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGC                                                                                                                                       <  SRR19973080.2446831/150‑1 (MQ=60)

                 GGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAA                                                                                                                                    >  SRR19973080.421011/1‑150 (MQ=60)

                    TTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTC                                                                                                                                 >  SRR19973080.3252412/1‑150 (MQ=60)

                    TTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTC                                                                                                                                 <  SRR19973080.458239/150‑1 (MQ=60)

                    TTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTC                                                                                                                                 >  SRR19973080.965178/1‑150 (MQ=60)

                    TTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTC                                                                                                                                 <  SRR19973080.2736619/150‑1 (MQ=60)

                       CATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAG                                                                                                                              <  SRR19973080.3962076/150‑1 (MQ=60)

                        ATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGC                                                                                                                             <  SRR19973080.1644237/150‑1 (MQ=60)

                        ATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGC                                                                                                                             <  SRR19973080.323555/150‑1 (MQ=60)

                        ATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGC                                                                                                                             >  SRR19973080.1906756/1‑150 (MQ=60)

                         TCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCA                                                                                                                            >  SRR19973080.541739/1‑150 (MQ=60)

                         TCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCA                                                                                                                            >  SRR19973080.541959/1‑150 (MQ=60)

                             CCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATA                                                                                                                        <  SRR19973080.46644/150‑1 (MQ=60)

                                CAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTA                                                                                                                     <  SRR19973080.3899275/150‑1 (MQ=60)

                                CAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTA                                                                                                                     <  SRR19973080.1002958/150‑1 (MQ=60)

                                CAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTA                                                                                                                     <  SRR19973080.1006698/150‑1 (MQ=60)

                                CAGTGATATCTGCTTGCTGCGCAGACGTAATAAGGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTA                                                                                                                     <  SRR19973080.1006323/150‑1 (MQ=60)

                                   TGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCC                                                                                                                  <  SRR19973080.3061344/150‑1 (MQ=60)

                                   TGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCC                                                                                                                  >  SRR19973080.431211/1‑150 (MQ=60)

                                     ATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAC                                                                                                                >  SRR19973080.3438215/1‑150 (MQ=60)

                                        TCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACT                                                                                                             >  SRR19973080.992796/1‑150 (MQ=60)

                                         CTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTT                                                                                                            <  SRR19973080.884400/150‑1 (MQ=60)

                                         CTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTT                                                                                                            <  SRR19973080.884365/150‑1 (MQ=60)

                                         CTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTT                                                                                                            <  SRR19973080.3164687/150‑1 (MQ=60)

                                         CTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTT                                                                                                            <  SRR19973080.3164601/150‑1 (MQ=60)

                                          TGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTA                                                                                                           >  SRR19973080.3345279/1‑150 (MQ=60)

                                          TGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTA                                                                                                           >  SRR19973080.3275078/1‑150 (MQ=60)

                                            CTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATA                                                                                                         >  SRR19973080.2910237/1‑150 (MQ=60)

                                             TTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAG                                                                                                        >  SRR19973080.924510/1‑150 (MQ=60)

                                              TGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGG                                                                                                       <  SRR19973080.1180645/150‑1 (MQ=60)

                                              TGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGG                                                                                                       <  SRR19973080.2499282/150‑1 (MQ=60)

                                              TGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGG                                                                                                       >  SRR19973080.166432/1‑150 (MQ=60)

                                                  GCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGC                                                                                                   <  SRR19973080.316039/150‑1 (MQ=60)

                                                   CTCAGACGTAATAATGCTCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCG                                                                                                  <  SRR19973080.3811673/150‑1 (MQ=60)

                                                   CGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCG                                                                                                  <  SRR19973080.3811574/150‑1 (MQ=60)

                                                   CGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCG                                                                                                  <  SRR19973080.3811679/150‑1 (MQ=60)

                                                        ACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAG                                                                                             >  SRR19973080.3056668/1‑150 (MQ=60)

                                                         CGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGA                                                                                            >  SRR19973080.4056885/1‑150 (MQ=60)

                                                         CGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGA                                                                                            >  SRR19973080.1987278/1‑150 (MQ=60)

                                                         CGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGA                                                                                            <  SRR19973080.4368195/150‑1 (MQ=60)

                                                            AATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAA                                                                                         >  SRR19973080.3874025/1‑150 (MQ=60)

                                                              TAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACG                                                                                       <  SRR19973080.2657574/150‑1 (MQ=60)

                                                              TAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACG                                                                                       <  SRR19973080.2659303/150‑1 (MQ=60)

                                                                 TGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGT                                                                                    >  SRR19973080.4479745/1‑150 (MQ=60)

                                                                   CGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTA                                                                                  <  SRR19973080.1794250/150‑1 (MQ=60)

                                                                     CTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAAC                                                                                >  SRR19973080.2375503/1‑150 (MQ=60)

                                                                       CTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACAC                                                                              >  SRR19973080.580813/1‑150 (MQ=60)

                                                                           TTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCT                                                                          <  SRR19973080.3147563/150‑1 (MQ=60)

                                                                           TTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCT                                                                          <  SRR19973080.1606342/150‑1 (MQ=60)

                                                                           TTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCT                                                                          <  SRR19973080.3146848/150‑1 (MQ=60)

                                                                           TTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCT                                                                          <  SRR19973080.2471621/150‑1 (MQ=60)

                                                                           TTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCT                                                                          <  SRR19973080.2471748/150‑1 (MQ=60)

                                                                            TTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTG                                                                         >  SRR19973080.883940/1‑150 (MQ=60)

                                                                            TTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTG                                                                         <  SRR19973080.1762519/150‑1 (MQ=60)

                                                                              AAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCA                                                                       >  SRR19973080.38789/1‑150 (MQ=60)

                                                                              AAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCA                                                                       >  SRR19973080.38759/1‑150 (MQ=60)

                                                                                   ACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATT                                                                  <  SRR19973080.4421841/150‑1 (MQ=60)

                                                                                   ACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATT                                                                  <  SRR19973080.2195889/150‑1 (MQ=60)

                                                                                   ACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATT                                                                  <  SRR19973080.2195850/150‑1 (MQ=60)

                                                                                   ACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATC                                                                  <  SRR19973080.2195717/150‑1 (MQ=60)

                                                                                      CTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAG                                                               >  SRR19973080.1845509/1‑150 (MQ=60)

                                                                                           GCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAA                                                          >  SRR19973080.395405/1‑150 (MQ=60)

                                                                                            CCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAA                                                         <  SRR19973080.2528489/150‑1 (MQ=60)

                                                                                            CCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAA                                                         <  SRR19973080.2527301/150‑1 (MQ=60)

                                                                                            CCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAA                                                         <  SRR19973080.3250331/150‑1 (MQ=60)

                                                                                            CCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAA                                                         <  SRR19973080.3250372/150‑1 (MQ=60)

                                                                                            CCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAA                                                         <  SRR19973080.3250394/150‑1 (MQ=60)

                                                                                             CCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAAT                                                        <  SRR19973080.3951822/150‑1 (MQ=60)

                                                                                              CGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATC                                                       >  SRR19973080.396472/1‑150 (MQ=60)

                                                                                              CGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATA                                                       >  SRR19973080.396338/1‑150 (MQ=60)

                                                                                              CGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATA                                                       >  SRR19973080.396320/1‑150 (MQ=60)

                                                                                              CGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATA                                                       <  SRR19973080.615817/150‑1 (MQ=60)

                                                                                              CGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATA                                                       <  SRR19973080.614767/150‑1 (MQ=60)

                                                                                                TGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAAC                                                     <  SRR19973080.244041/150‑1 (MQ=60)

                                                                                                TGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAAC                                                     >  SRR19973080.3226441/1‑150 (MQ=60)

                                                                                                TGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAAC                                                     <  SRR19973080.2521973/150‑1 (MQ=60)

                                                                                                  CGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGT                                                   >  SRR19973080.1067207/1‑150 (MQ=60)

                                                                                                   GGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTG                                                  >  SRR19973080.1848724/1‑150 (MQ=60)

                                                                                                    GTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGT                                                 <  SRR19973080.2711300/150‑1 (MQ=60)

                                                                                                        CCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACT                                             <  SRR19973080.10157/150‑1 (MQ=60)

                                                                                                          AGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTG                                           <  SRR19973080.792388/150‑1 (MQ=60)

                                                                                                          AGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTG                                           >  SRR19973080.1719097/1‑150 (MQ=60)

                                                                                                            ATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGAT                                         <  SRR19973080.2865181/150‑1 (MQ=60)

                                                                                                            ATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGAT                                         <  SRR19973080.2866395/150‑1 (MQ=60)

                                                                                                                TTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCT                                     >  SRR19973080.3959052/1‑150 (MQ=60)

                                                                                                                TTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCT                                     <  SRR19973080.1386762/150‑1 (MQ=60)

                                                                                                                 TGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTC                                    >  SRR19973080.4030048/1‑150 (MQ=60)

                                                                                                                 TGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTC                                    >  SRR19973080.4030110/1‑150 (MQ=60)

                                                                                                                  GGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCTGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCT                                   >  SRR19973080.3174652/1‑150 (MQ=60)

                                                                                                                  GGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCT                                   >  SRR19973080.3052476/1‑150 (MQ=60)

                                                                                                                  GGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCT                                   >  SRR19973080.3052457/1‑150 (MQ=60)

                                                                                                                  GGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCT                                   <  SRR19973080.4425101/150‑1 (MQ=60)

                                                                                                                   GTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTC                                  <  SRR19973080.743488/150‑1 (MQ=60)

                                                                                                                   GTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTC                                  >  SRR19973080.1796715/1‑150 (MQ=60)

                                                                                                                       ACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACA                              >  SRR19973080.1235174/1‑150 (MQ=60)

                                                                                                                       ACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACA                              >  SRR19973080.1440627/1‑150 (MQ=60)

                                                                                                                         TGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACC                            >  SRR19973080.3280806/1‑150 (MQ=60)

                                                                                                                           CTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTA                          <  SRR19973080.3769836/150‑1 (MQ=60)

                                                                                                                           CTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTA                          >  SRR19973080.2066657/1‑150 (MQ=60)

                                                                                                                           CTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTA                          <  SRR19973080.3917024/150‑1 (MQ=60)

                                                                                                                             GATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGAGAAATAACGTGTAACTTGATGTCTCTCAACACCTACA                        >  SRR19973080.4484320/1‑150 (MQ=60)

                                                                                                                              ATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAG                       <  SRR19973080.965762/150‑1 (MQ=60)

                                                                                                                              ATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAG                       <  SRR19973080.964490/150‑1 (MQ=60)

                                                                                                                                AAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGAT                     <  SRR19973080.316361/150‑1 (MQ=60)

                                                                                                                                AAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGAT                     <  SRR19973080.2593891/150‑1 (MQ=60)

                                                                                                                                AAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGAT                     >  SRR19973080.3382924/1‑150 (MQ=60)

                                                                                                                                  AATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAG                   <  SRR19973080.3848846/150‑1 (MQ=60)

                                                                                                                                    TTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTA                 >  SRR19973080.2000499/1‑150 (MQ=60)

                                                                                                                                    TTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTA                 <  SRR19973080.4521284/150‑1 (MQ=60)

                                                                                                                                    TTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTA                 <  SRR19973080.497793/150‑1 (MQ=60)

                                                                                                                                      CTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGA               <  SRR19973080.496078/150‑1 (MQ=60)

                                                                                                                                      CTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGA               >  SRR19973080.3819385/1‑150 (MQ=60)

                                                                                                                                        CCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAA             <  SRR19973080.2989790/150‑1 (MQ=60)

                                                                                                                                         CACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAA            >  SRR19973080.2739020/1‑150 (MQ=60)

                                                                                                                                            GCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACT         <  SRR19973080.1028339/150‑1 (MQ=60)

                                                                                                                                            GCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACT         <  SRR19973080.1028256/150‑1 (MQ=60)

                                                                                                                                              ATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGT       >  SRR19973080.1786519/1‑150 (MQ=60)

                                                                                                                                                TGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTAC     <  SRR19973080.3106861/150‑1 (MQ=60)

                                                                                                                                                 GCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACA    >  SRR19973080.3496693/1‑150 (MQ=60)

                                                                                                                                                  CTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAG   >  SRR19973080.4413386/1‑150 (MQ=60)

                                                                                                                                                   TATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGG  >  SRR19973080.103581/1‑150 (MQ=60)

ACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGT  >  CP009273/3785768‑3786064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`