Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F204 I0 R1	1155	255.8	8356456	95.7%	7997128	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,785,915	G→T	100%	intergenic (‑4/+271)	kbl ← / ← yibB	glycine C‑acetyltransferase/YibB family protein, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,785,915	0	G	T	99.2%	879.6 / ‑1.2	248	intergenic (‑4/+271)	kbl/yibB	glycine C‑acetyltransferase/YibB family protein, function unknown
Reads supporting (aligned to +/- strand): ref base G (0/2); new base T (125/121); total (125/123)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 248 total aligned reads displayed.

ACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTAC  >  CP009273/3785768‑3786061

  aaaaGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATtc                                                                                                                                                >  2:3514897/1‑150 (MQ=255)

    aaGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATtctc                                                                                                                                              >  2:1929601/1‑150 (MQ=255)

    aaGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATtctc                                                                                                                                              <  2:2633662/150‑1 (MQ=255)

     aGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTcc                                                                                                                                             >  1:216506/1‑150 (MQ=255)

      gTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCa                                                                                                                                            >  1:4067578/1‑150 (MQ=255)

      gTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCa                                                                                                                                            <  1:3151008/150‑1 (MQ=255)

      gTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCa                                                                                                                                            <  1:2123910/150‑1 (MQ=255)

            gACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAgg                                                                                                                                      >  2:2940483/1‑150 (MQ=255)

               gTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCa                                                                                                                                   >  2:2686338/1‑150 (MQ=255)

                  gCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAAt                                                                                                                                >  1:678881/1‑150 (MQ=255)

                  gCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAAt                                                                                                                                <  2:2606004/150‑1 (MQ=255)

                   cTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAAtt                                                                                                                               <  1:396994/150‑1 (MQ=255)

                    ttCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTc                                                                                                                              >  1:3886979/1‑150 (MQ=255)

                    ttCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTc                                                                                                                              >  2:3881875/1‑150 (MQ=255)

                    ttCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTc                                                                                                                              >  2:742321/1‑150 (MQ=255)

                           aGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTACCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGcaca                                                                                                                       <  1:3095880/150‑1 (MQ=255)

                            gCCACAGTGATATCTTCTTGCTGCGCAGAAGTAATAATGAGATCTTCTTTAAACAAAACTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACat                                                                                                                      <  1:3113907/150‑1 (MQ=255)

                              cacaGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACatat                                                                                                                    <  2:2377356/150‑1 (MQ=255)

                              cacaGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACatat                                                                                                                    >  1:2329804/1‑150 (MQ=255)

                                 aGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTAc                                                                                                                 <  2:2128280/150‑1 (MQ=255)

                                 aGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTAc                                                                                                                 <  1:2854866/150‑1 (MQ=255)

                                  gTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTAcc                                                                                                                <  2:1236769/150‑1 (MQ=255)

                                   tGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTAccc                                                                                                               <  1:856718/150‑1 (MQ=255)

                                   tGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTAccc                                                                                                               <  1:1545719/150‑1 (MQ=255)

                                    gATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCa                                                                                                              <  1:2155/150‑1 (MQ=255)

                                    gATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCa                                                                                                              <  1:2925806/150‑1 (MQ=255)

                                     atatCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCaa                                                                                                             >  2:2827465/1‑150 (MQ=255)

                                     atatCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCaa                                                                                                             >  2:2827517/1‑150 (MQ=255)

                                        tCTGCTTGATGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCTTGAGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTAACCAAACt                                                                                                          <  1:141346/150‑1 (MQ=255)

                                          tgcttgctGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTta                                                                                                        <  1:3386523/150‑1 (MQ=255)

                                            cttgctGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTtata                                                                                                      >  2:732753/1‑150 (MQ=255)

                                             ttgctGCGAAGACGTATTAATGCGCTATTCTTTAAACAACCATTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACTCATTGCTATTCTCCAGTCAGTGCATATTCCAGCACATATTACCCAAACTTATAg                                                                                                     <  2:2644619/150‑1 (MQ=255)

                                                 tgcGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTcg                                                                                                 >  1:3356399/1‑150 (MQ=255)

                                                   cgcAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTcgcg                                                                                               <  1:731793/150‑1 (MQ=255)

                                                     cAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGcgac                                                                                             <  2:682309/150‑1 (MQ=255)

                                                     cAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGcgac                                                                                             <  2:4087151/150‑1 (MQ=255)

                                                      aGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGcgacg                                                                                            >  1:2472253/1‑150 (MQ=255)

                                                       gACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGcgacga                                                                                           >  1:2732536/1‑150 (MQ=255)

                                                       gACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGcgacga                                                                                           >  2:3078/1‑150 (MQ=255)

                                                          gTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGAt                                                                                        >  2:2622495/1‑150 (MQ=255)

                                                          gTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGAt                                                                                        >  1:2302929/1‑150 (MQ=255)

                                                            aataatGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTTATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATaa                                                                                      <  2:3655747/150‑1 (MQ=255)

                                                            aataatGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATaa                                                                                      <  2:3375074/150‑1 (MQ=255)

                                                            aataatGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATaa                                                                                      <  2:952224/150‑1 (MQ=255)

                                                            aataatGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATaa                                                                                      >  1:1107416/1‑150 (MQ=255)

                                                            aataatGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATaa                                                                                      <  1:2117348/150‑1 (MQ=255)

                                                             ataatGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAAc                                                                                     >  1:1690184/1‑150 (MQ=255)

                                                             ataatGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAAc                                                                                     <  2:3617748/150‑1 (MQ=255)

                                                                atGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAActcg                                                                                  >  1:1739005/1‑150 (MQ=255)

                                                                atGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAAcgcg                                                                                  >  2:3738567/1‑150 (MQ=255)

                                                                atGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAAcgcg                                                                                  <  2:3296210/150‑1 (MQ=255)

                                                                   cgcTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTa                                                                               <  2:2515626/150‑1 (MQ=255)

                                                                        ttcttTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACt                                                                          <  1:584140/150‑1 (MQ=255)

                                                                          cttTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTc                                                                        >  1:1979539/1‑150 (MQ=255)

                                                                           tttAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCt                                                                       <  1:985079/150‑1 (MQ=255)

                                                                                 caacCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCaaaa                                                                 >  1:1572936/1‑150 (MQ=255)

                                                                                  aacCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAAt                                                                <  1:618322/150‑1 (MQ=255)

                                                                                  aacCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAAt                                                                >  2:1818314/1‑150 (MQ=255)

                                                                                        tCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGa                                                          <  1:1182472/150‑1 (MQ=255)

                                                                                         ccGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGat                                                         <  2:3968017/150‑1 (MQ=255)

                                                                                          cGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGata                                                        <  1:1821494/150‑1 (MQ=255)

                                                                                          cGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGata                                                        >  1:3080249/1‑150 (MQ=255)

                                                                                          cGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGata                                                        >  2:1484408/1‑150 (MQ=255)

                                                                                            cccGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGataaa                                                      >  2:1623194/1‑150 (MQ=255)

                                                                                             ccGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGataaat                                                     <  2:1891994/150‑1 (MQ=255)

                                                                                              cGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGataaata                                                    <  2:489763/150‑1 (MQ=255)

                                                                                              cGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGataaata                                                    <  2:3155195/150‑1 (MQ=255)

                                                                                              cGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGataaata                                                    <  1:3042516/150‑1 (MQ=255)

                                                                                                   ggTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACgtg                                               >  1:845606/1‑150 (MQ=255)

                                                                                                   ggTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACggg                                               >  2:2723910/1‑148 (MQ=255)

                                                                                                        ccAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGTCTAAATAACGTGTAACt                                          >  1:2885080/1‑150 (MQ=255)

                                                                                                         cAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACtt                                         <  2:1757426/150‑1 (MQ=255)

                                                                                                           gATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGa                                       <  1:2544103/150‑1 (MQ=255)

                                                                                                                 tGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGtctc                                 >  1:2087831/1‑150 (MQ=255)

                                                                                                                 tGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGtctc                                 <  2:597793/150‑1 (MQ=255)

                                                                                                                  ggTTAACTGCTGATAAAATTCTCCAAGCATTGCTATTCTCCAGACCGGGCAAATTCCAGCACAAATTACCCTAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGtctct                                >  2:4061535/1‑150 (MQ=255)

                                                                                                                   gTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGtctctc                               >  1:1218113/1‑150 (MQ=255)

                                                                                                                   gTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGtctctc                               >  1:3624052/1‑150 (MQ=255)

                                                                                                                    ttAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCa                              <  1:2949713/150‑1 (MQ=255)

                                                                                                                      aaCTGCTGATAAAATTCTCCACGAATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAac                            <  2:3638591/150‑1 (MQ=255)

                                                                                                                         tgctgATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACAcc                         <  2:1054459/150‑1 (MQ=255)

                                                                                                                         tgctgATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACAcc                         >  2:363065/1‑150 (MQ=255)

                                                                                                                             gATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACa                     <  2:2318395/150‑1 (MQ=255)

                                                                                                                             gATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACa                     >  1:459586/1‑150 (MQ=255)

                                                                                                                             gATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACa                     <  1:105831/150‑1 (MQ=255)

                                                                                                                              aTAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAg                    <  1:325620/150‑1 (MQ=255)

                                                                                                                                aaaaTTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGAt                  <  2:322686/150‑1 (MQ=255)

                                                                                                                                 aaaTTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGAta                 >  1:1800546/1‑150 (MQ=255)

                                                                                                                                  aaTTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGAtag                >  2:2250169/1‑150 (MQ=255)

                                                                                                                                    ttCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGAtagta              >  1:926551/1‑150 (MQ=255)

                                                                                                                                       tcCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGaa           >  2:129801/1‑150 (MQ=255)

                                                                                                                                         cACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGaaaa         <  1:3652472/150‑1 (MQ=255)

                                                                                                                                            gCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACt      <  2:3037703/150‑1 (MQ=255)

                                                                                                                                            gCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACt      >  2:3009884/1‑150 (MQ=255)

                                                                                                                                             cATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAATACTg     >  2:413949/1‑150 (MQ=255)

                                                                                                                                             cATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTg     >  1:559533/1‑150 (MQ=255)

                                                                                                                                             cATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTg     <  1:2007854/150‑1 (MQ=255)

                                                                                                                                              aTTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGt    >  1:1887687/1‑150 (MQ=255)

                                                                                                                                              aTTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGt    >  2:711287/1‑150 (MQ=255)

                                                                                                                                              aTTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGt    >  1:3931564/1‑150 (MQ=255)

ACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTAC  >  CP009273/3785768‑3786061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGT  >  CP009273/3785766‑3786064

GCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCT                                                                                                                                                       <  SRR19973074.964458/150‑1 (MQ=60)

GCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCT                                                                                                                                                       <  SRR19973074.1325837/150‑1 (MQ=60)

GCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCT                                                                                                                                                       <  SRR19973074.543156/150‑1 (MQ=60)

 CACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTA                                                                                                                                                      <  SRR19973074.3123377/150‑1 (MQ=60)

       AGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCC                                                                                                                                                >  SRR19973074.1900432/1‑150 (MQ=60)

       AGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCC                                                                                                                                                <  SRR19973074.200544/150‑1 (MQ=60)

       AGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCC                                                                                                                                                >  SRR19973074.216953/1‑150 (MQ=60)

       AGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCC                                                                                                                                                >  SRR19973074.216954/1‑150 (MQ=60)

       AGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCC                                                                                                                                                <  SRR19973074.3932452/150‑1 (MQ=60)

       AGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGGTGGTTAACTGCTGATAAAAGTCTCCACGCATTGCTATTCTCC                                                                                                                                                >  SRR19973074.216957/1‑150 (MQ=60)

        GTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCA                                                                                                                                               <  SRR19973074.3156794/150‑1 (MQ=60)

        GTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCA                                                                                                                                               <  SRR19973074.2128134/150‑1 (MQ=60)

        GTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCA                                                                                                                                               <  SRR19973074.2127897/150‑1 (MQ=60)

        GTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCA                                                                                                                                               >  SRR19973074.4075100/1‑150 (MQ=60)

        GTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCA                                                                                                                                               >  SRR19973074.4075204/1‑150 (MQ=60)

         TTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAG                                                                                                                                              <  SRR19973074.736983/150‑1 (MQ=60)

          TAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGA                                                                                                                                             >  SRR19973074.909345/1‑150 (MQ=60)

           AATGACGTGGCTTACATCAGCAACAGTGATATCTGCTTGCTGCGCAGACGAAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGAGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATAGCTATTCTCCAGAC                                                                                                                                            >  SRR19973074.1256280/1‑150 (MQ=60)

                CGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGC                                                                                                                                       <  SRR19973074.512664/150‑1 (MQ=60)

                  TGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAA                                                                                                                                     >  SRR19973074.401680/1‑150 (MQ=60)

                    GCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAAT                                                                                                                                   >  SRR19973074.680132/1‑150 (MQ=60)

                    GCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAAT                                                                                                                                   >  SRR19973074.679872/1‑150 (MQ=60)

                     CTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATT                                                                                                                                  >  SRR19973074.4124552/1‑150 (MQ=60)

                     CTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATT                                                                                                                                  <  SRR19973074.1594573/150‑1 (MQ=60)

                     CTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATT                                                                                                                                  >  SRR19973074.4124575/1‑150 (MQ=60)

                     CTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATT                                                                                                                                  <  SRR19973074.397750/150‑1 (MQ=60)

                     CTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATT                                                                                                                                  >  SRR19973074.4124549/1‑150 (MQ=60)

                      TTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTC                                                                                                                                 >  SRR19973074.3894101/1‑150 (MQ=60)

                         CATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAG                                                                                                                              <  SRR19973074.143852/150‑1 (MQ=60)

                             AGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTACCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACA                                                                                                                          <  SRR19973074.3101565/150‑1 (MQ=60)

                              GCCACAGTGATATCTTCTTGCTGCGCAGAAGTAATAATGAGATCTTCTTTAAACAAAACTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACAT                                                                                                                         <  SRR19973074.3119620/150‑1 (MQ=60)

                              GCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACAT                                                                                                                         <  SRR19973074.2778432/150‑1 (MQ=60)

                               CCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATA                                                                                                                        <  SRR19973074.177504/150‑1 (MQ=60)

                                CACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATAT                                                                                                                       <  SRR19973074.2640849/150‑1 (MQ=60)

                                CACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATAT                                                                                                                       >  SRR19973074.2334150/1‑150 (MQ=60)

                                CACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATAT                                                                                                                       >  SRR19973074.2333982/1‑150 (MQ=60)

                                   AGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTAC                                                                                                                    <  SRR19973074.2860159/150‑1 (MQ=60)

                                   AGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTAC                                                                                                                    <  SRR19973074.2860169/150‑1 (MQ=60)

                                     TGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCC                                                                                                                  <  SRR19973074.858357/150‑1 (MQ=60)

                                     TGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCC                                                                                                                  <  SRR19973074.858369/150‑1 (MQ=60)

                                     TGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCC                                                                                                                  <  SRR19973074.1548652/150‑1 (MQ=60)

                                      GATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCA                                                                                                                 <  SRR19973074.2161/150‑1 (MQ=60)

                                      GATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCA                                                                                                                 <  SRR19973074.3097494/150‑1 (MQ=60)

                                      GATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCA                                                                                                                 <  SRR19973074.2159/150‑1 (MQ=60)

                                      GATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCA                                                                                                                 <  SRR19973074.2132/150‑1 (MQ=60)

                                      GATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCA                                                                                                                 <  SRR19973074.2126/150‑1 (MQ=60)

                                      GATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCA                                                                                                                 <  SRR19973074.2896829/150‑1 (MQ=60)

                                      GATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCA                                                                                                                 <  SRR19973074.2931249/150‑1 (MQ=60)

                                        TATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAA                                                                                                               >  SRR19973074.2102881/1‑150 (MQ=60)

                                          TCTGCTTGATGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCTTGAGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTAACCAAACT                                                                                                             <  SRR19973074.141670/150‑1 (MQ=60)

                                            TGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTA                                                                                                           <  SRR19973074.3392759/150‑1 (MQ=60)

                                            TGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTA                                                                                                           <  SRR19973074.3392791/150‑1 (MQ=60)

                                              CTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATA                                                                                                         <  SRR19973074.618706/150‑1 (MQ=60)

                                                   TGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCG                                                                                                    >  SRR19973074.3362625/1‑150 (MQ=60)

                                                     CGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCG                                                                                                  <  SRR19973074.733167/150‑1 (MQ=60)

                                                       CAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGAC                                                                                                <  SRR19973074.2368389/150‑1 (MQ=60)

                                                        AGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACG                                                                                               >  SRR19973074.2476840/1‑150 (MQ=60)

                                                        AGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACG                                                                                               >  SRR19973074.2476836/1‑150 (MQ=60)

                                                         GACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGA                                                                                              >  SRR19973074.2737579/1‑150 (MQ=60)

                                                           CGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGA                                                                                            >  SRR19973074.3622922/1‑150 (MQ=60)

                                                           CGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGA                                                                                            >  SRR19973074.583223/1‑150 (MQ=60)

                                                           CGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGA                                                                                            >  SRR19973074.583817/1‑150 (MQ=60)

                                                            GTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGAT                                                                                           >  SRR19973074.2307235/1‑150 (MQ=60)

                                                              AATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAA                                                                                         >  SRR19973074.2890422/1‑150 (MQ=60)

                                                              AATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAA                                                                                         <  SRR19973074.2121323/150‑1 (MQ=60)

                                                              AATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAA                                                                                         >  SRR19973074.146513/1‑150 (MQ=60)

                                                              AATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAA                                                                                         >  SRR19973074.1109498/1‑150 (MQ=60)

                                                               ATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAAC                                                                                        >  SRR19973074.1693342/1‑150 (MQ=60)

                                                                  ATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACTCG                                                                                     >  SRR19973074.1742224/1‑150 (MQ=60)

                                                                  ATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCG                                                                                     <  SRR19973074.3268393/150‑1 (MQ=60)

                                                                    GCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTT                                                                                   <  SRR19973074.3142567/150‑1 (MQ=60)

                                                                    GCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTT                                                                                   <  SRR19973074.3142543/150‑1 (MQ=60)

                                                                    GCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTT                                                                                   <  SRR19973074.1845594/150‑1 (MQ=60)

                                                                          TTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACT                                                                             <  SRR19973074.585268/150‑1 (MQ=60)

                                                                            CTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTC                                                                           >  SRR19973074.1983305/1‑150 (MQ=60)

                                                                            CTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTC                                                                           >  SRR19973074.1983355/1‑150 (MQ=60)

                                                                             TTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCT                                                                          <  SRR19973074.986921/150‑1 (MQ=60)

                                                                               TAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGC                                                                        <  SRR19973074.1619560/150‑1 (MQ=60)

                                                                                   CAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAA                                                                    <  SRR19973074.2058174/150‑1 (MQ=60)

                                                                                   CAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAA                                                                    >  SRR19973074.1575922/1‑150 (MQ=60)

                                                                                    AACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAAT                                                                   <  SRR19973074.619491/150‑1 (MQ=60)

                                                                                     ACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATT                                                                  >  SRR19973074.3657510/1‑150 (MQ=60)

                                                                                       CCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCA                                                                >  SRR19973074.3703605/1‑150 (MQ=60)

                                                                                         TTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGG                                                              >  SRR19973074.641828/1‑150 (MQ=60)

                                                                                          TCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGA                                                             <  SRR19973074.1184728/150‑1 (MQ=60)

                                                                                            CGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATA                                                           <  SRR19973074.1824966/150‑1 (MQ=60)

                                                                                            CGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATA                                                           <  SRR19973074.2559087/150‑1 (MQ=60)

                                                                                            CGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATA                                                           >  SRR19973074.3085971/1‑150 (MQ=60)

                                                                                            CGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATA                                                           >  SRR19973074.3085915/1‑150 (MQ=60)

                                                                                                CGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATA                                                       <  SRR19973074.3157557/150‑1 (MQ=60)

                                                                                                CGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATA                                                       <  SRR19973074.3048353/150‑1 (MQ=60)

                                                                                                CGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATA                                                       <  SRR19973074.3048142/150‑1 (MQ=60)

                                                                                                  TGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAAC                                                     >  SRR19973074.1176721/1‑150 (MQ=60)

                                                                                                   GCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATAGTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACG                                                    >  SRR19973074.3103743/1‑150 (MQ=60)

                                                                                                    CGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGT                                                   >  SRR19973074.1567452/1‑150 (MQ=60)

                                                                                                     GGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTG                                                  >  SRR19973074.847236/1‑150 (MQ=60)

                                                                                                     GGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTG                                                  >  SRR19973074.846806/1‑150 (MQ=60)

                                                                                                         TCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAAC                                              <  SRR19973074.1212362/150‑1 (MQ=60)

                                                                                                          CCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGTCTAAATAACGTGTAACT                                             >  SRR19973074.2890432/1‑150 (MQ=60)

                                                                                                            AGATCGTTGGTTAACTGCTGATAAAATTCTNCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTG                                           >  SRR19973074.3861706/1‑150 (MQ=60)

                                                                                                             GATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGA                                          <  SRR19973074.2548851/150‑1 (MQ=60)

                                                                                                             GATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGA                                          <  SRR19973074.3359775/150‑1 (MQ=60)

                                                                                                                   TGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTC                                    >  SRR19973074.2091840/1‑150 (MQ=60)

                                                                                                                   TGGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTC                                    >  SRR19973074.2091741/1‑150 (MQ=60)

                                                                                                                    GGTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCT                                   >  SRR19973074.905774/1‑150 (MQ=60)

                                                                                                                     GTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGTGCAAATTCCAGCAAATATTACCAAAACTTATAGTTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTC                                  >  SRR19973074.1220424/1‑150 (MQ=60)

                                                                                                                     GTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTC                                  >  SRR19973074.1220450/1‑150 (MQ=60)

                                                                                                                     GTTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTC                                  >  SRR19973074.3630748/1‑150 (MQ=60)

                                                                                                                      TTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCA                                 <  SRR19973074.2955125/150‑1 (MQ=60)

                                                                                                                      TTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCA                                 <  SRR19973074.2955197/150‑1 (MQ=60)

                                                                                                                      TTAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCA                                 >  SRR19973074.107404/1‑150 (MQ=60)

                                                                                                                       TAACTGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAA                                >  SRR19973074.3940995/1‑150 (MQ=60)

                                                                                                                           TGCTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACC                            <  SRR19973074.2994821/150‑1 (MQ=60)

                                                                                                                             CTGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTA                          >  SRR19973074.2528736/1‑150 (MQ=60)

                                                                                                                              TGATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTAC                         <  SRR19973074.3521353/150‑1 (MQ=60)

                                                                                                                               GATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACA                        >  SRR19973074.460487/1‑150 (MQ=60)

                                                                                                                               GATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACA                        <  SRR19973074.106086/150‑1 (MQ=60)

                                                                                                                                ATAAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAG                       <  SRR19973074.326212/150‑1 (MQ=60)

                                                                                                                                  AAAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGG                                                                                                       <  SRR19973074.2416210/68‑1 (MQ=60)

                                                                                                                                   AAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATA                    >  SRR19973074.1803939/1‑150 (MQ=60)

                                                                                                                                   AAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATA                    >  SRR19973074.1803969/1‑150 (MQ=60)

                                                                                                                                   AAATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATA                    >  SRR19973074.1804024/1‑150 (MQ=60)

                                                                                                                                     ATTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGT                  <  SRR19973074.975278/150‑1 (MQ=60)

                                                                                                                                      TTCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTA                 >  SRR19973074.928313/1‑150 (MQ=60)

                                                                                                                                       TCTCCACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAG                <  SRR19973074.2957759/150‑1 (MQ=60)

                                                                                                                                           CACGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAA            <  SRR19973074.3659232/150‑1 (MQ=60)

                                                                                                                                             CGCATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACATCTACAGATAGTAGAAAAAC          <  SRR19973074.428015/150‑1 (MQ=60)

                                                                                                                                               CATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTG        <  SRR19973074.2011660/150‑1 (MQ=60)

                                                                                                                                               CATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTG        >  SRR19973074.560632/1‑150 (MQ=60)

                                                                                                                                               CATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTG        <  SRR19973074.1705889/150‑1 (MQ=60)

                                                                                                                                                ATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGT       >  SRR19973074.1891299/1‑150 (MQ=60)

                                                                                                                                                ATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGT       >  SRR19973074.682078/1‑150 (MQ=60)

                                                                                                                                                ATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGT       >  SRR19973074.3938803/1‑150 (MQ=60)

                                                                                                                                                ATTGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGT       >  SRR19973074.3938804/1‑150 (MQ=60)

                                                                                                                                                  TGCTATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTAC     <  SRR19973074.1889080/150‑1 (MQ=60)

                                                                                                                                                     TATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGT  >  SRR19973074.2937948/1‑150 (MQ=60)

                                                                                                                                                     TATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGT  <  SRR19973074.936016/150‑1 (MQ=60)

GCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGGATAAATAACGTGTAACTTGATGTCTCTCAACACCTACAGATAGTAGAAAAACTGTACAGT  >  CP009273/3785766‑3786064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`