Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,850,839 G→T 18.0% P309Q (CCG→CAG)  yidJ ← sulfatase/phosphatase superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,850,8390GT18.0% 649.8 / 106.6 227P309Q (CCG→CAG) yidJsulfatase/phosphatase superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (80/106);  new base T (27/14);  total (107/120)
Fisher's exact test for biased strand distribution p-value = 9.48e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

TGGCTCTTTCACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGTCCGATTTGGTC  >  CP009273/3850691‑3850987
                                                                                                                                                    |                                                                                                                                                    
tGGCTCTTTCACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCgg                                                                                                                                                     >  1:2502626/1‑150 (MQ=255)
   ctctTTCACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAAt                                                                                                                                                  <  1:4810358/150‑1 (MQ=255)
   ctctTTCACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAAt                                                                                                                                                  <  1:286461/150‑1 (MQ=255)
    tctTTCACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATg                                                                                                                                                 <  1:4609336/150‑1 (MQ=255)
    tctTTCACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATg                                                                                                                                                 >  2:2720458/1‑150 (MQ=255)
    tctTTCACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATg                                                                                                                                                 >  2:4058453/1‑150 (MQ=255)
         cACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGACAGCGCCATCATTGTCGGCAGTAAATCGATATTACTGACTGGCTTATCGACCTGAAGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGt                                                                                                                                            >  1:3024429/1‑150 (MQ=255)
          aCGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTg                                                                                                                                           >  1:2090838/1‑150 (MQ=255)
            ggCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGAt                                                                                                                                         <  1:2966431/150‑1 (MQ=255)
             gCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATg                                                                                                                                        <  1:4885684/150‑1 (MQ=255)
              cAAGGATATTGTCCCCCGGCAGAATATATTGTTTTTCAATATCTGCCAGCGCCATCATTGTCGTCTGTTTCTCGCTATGTCTGACTGGCTTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATgt                                                                                                                                       <  1:2656414/150‑1 (MQ=255)
               aaGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATgtc                                                                                                                                      <  1:4949840/150‑1 (MQ=255)
               aaGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATgtc                                                                                                                                      >  2:532487/1‑150 (MQ=255)
                aGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATgtcg                                                                                                                                     <  2:2854358/150‑1 (MQ=255)
                   atatTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCa                                                                                                                                  <  1:632523/150‑1 (MQ=255)
                   atatTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCa                                                                                                                                  <  2:1806493/150‑1 (MQ=255)
                    tatTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCAt                                                                                                                                 <  1:2535613/150‑1 (MQ=255)
                      ttttCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATAc                                                                                                                               >  2:1680969/1‑150 (MQ=255)
                         tCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATc                                                                                                                            <  2:1755586/150‑1 (MQ=255)
                          cccccGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATcg                                                                                                                           >  2:39971/1‑150 (MQ=255)
                            cccGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATcgcc                                                                                                                         <  2:2975264/150‑1 (MQ=255)
                            cccGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATcgcc                                                                                                                         <  1:4867436/150‑1 (MQ=255)
                              cGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATcgccgc                                                                                                                       >  1:501623/1‑150 (MQ=255)
                                 cAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGcccc                                                                                                                    >  2:671654/1‑150 (MQ=255)
                                  aGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCt                                                                                                                   <  2:2481468/150‑1 (MQ=255)
                                   gAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCtt                                                                                                                  <  1:3337767/150‑1 (MQ=255)
                                   gAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCtt                                                                                                                  <  2:1289899/150‑1 (MQ=255)
                                    aaTCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCttt                                                                                                                 >  2:4667512/1‑150 (MQ=255)
                                         ctGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGACTGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGa                                                                                                            >  2:27571/1‑150 (MQ=255)
                                            gTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCa                                                                                                         >  2:4221313/1‑150 (MQ=255)
                                                 tCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTGTACTGATCAGCTTa                                                                                                    >  1:1868018/1‑150 (MQ=255)
                                                  cAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTAt                                                                                                   >  2:1699371/1‑150 (MQ=255)
                                                      atCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTgc                                                                                               >  1:3189772/1‑150 (MQ=255)
                                                       tCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTgcg                                                                                              <  1:2526364/150‑1 (MQ=255)
                                                            cAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCcat                                                                                         >  2:2645478/1‑150 (MQ=255)
                                                             aGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCcatc                                                                                        >  2:3115412/1‑150 (MQ=255)
                                                              gcgcCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCcatca                                                                                       >  2:377118/1‑150 (MQ=255)
                                                               cgcCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCcatcat                                                                                      <  1:1252679/150‑1 (MQ=255)
                                                                gcCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCAtt                                                                                     <  1:3470472/150‑1 (MQ=255)
                                                                  catcatTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTc                                                                                   <  2:2574690/150‑1 (MQ=255)
                                                                   atcatTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCg                                                                                  >  2:429826/1‑150 (MQ=255)
                                                                      atTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCg                                                                               <  1:2013159/150‑1 (MQ=255)
                                                                       ttGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGt                                                                              <  2:4429918/150‑1 (MQ=255)
                                                                       ttGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGAGGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGt                                                                              <  2:1407223/150‑1 (MQ=255)
                                                                         gTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGa                                                                            >  2:613862/1‑150 (MQ=255)
                                                                           cGGCAGTAAAGCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATc                                                                          <  2:3359652/150‑1 (MQ=255)
                                                                              cAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCCGCTTATGTGCGCCCATCATTTCGCCGTGATCGGa                                                                       >  2:4918463/1‑150 (MQ=255)
                                                                               aGTAAATCGCTATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAg                                                                      <  2:2231948/150‑1 (MQ=255)
                                                                               aGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAg                                                                      >  2:1162961/1‑150 (MQ=255)
                                                                               aGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAg                                                                      >  2:1162938/1‑150 (MQ=255)
                                                                               aGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAg                                                                      >  1:103273/1‑150 (MQ=255)
                                                                                 tAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGt                                                                    >  1:3249179/1‑150 (MQ=255)
                                                                                   aaTCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGtat                                                                  <  2:1927224/150‑1 (MQ=255)
                                                                                   aaTCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGtat                                                                  <  1:2434041/150‑1 (MQ=255)
                                                                                      cGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTataaa                                                               <  1:996615/150‑1 (MQ=255)
                                                                                       gATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTataaat                                                              >  2:3586370/1‑150 (MQ=255)
                                                                                           tgactgacTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGACTGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAAcc                                                          >  2:2420358/1‑150 (MQ=255)
                                                                                           tgactgacTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAAcc                                                          >  2:4315626/1‑150 (MQ=255)
                                                                                               tgactgGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACg                                                      >  2:3846452/1‑150 (MQ=255)
                                                                                                  ctgGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTAATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTAt                                                   >  1:4438571/1‑150 (MQ=255)
                                                                                                   tgGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTAtt                                                  <  2:2380626/150‑1 (MQ=255)
                                                                                                    ggCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGATCGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAATTAACCCACGTAttt                                                 <  2:2597664/150‑1 (MQ=255)
                                                                                                    ggCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTAttt                                                 >  2:515887/1‑150 (MQ=255)
                                                                                                    ggCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTAttt                                                 <  2:5174869/150‑1 (MQ=255)
                                                                                                      cGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTc                                               >  1:1545580/1‑150 (MQ=255)
                                                                                                      cGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTc                                               <  2:4146377/150‑1 (MQ=255)
                                                                                                       gTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCa                                              <  1:532890/150‑1 (MQ=255)
                                                                                                       gTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCa                                              >  2:5053427/1‑150 (MQ=255)
                                                                                                       gTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCa                                              >  1:2169622/1‑150 (MQ=255)
                                                                                                       gTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCa                                              >  2:1881916/1‑150 (MQ=255)
                                                                                                              ccTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGct                                       >  1:5152888/1‑150 (MQ=255)
                                                                                                              ccTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGct                                       >  2:2259997/1‑150 (MQ=255)
                                                                                                                tGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGctct                                     >  2:746744/1‑150 (MQ=255)
                                                                                                                 gTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGCGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTg                                    <  1:4227427/150‑1 (MQ=255)
                                                                                                                  tCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTgg                                   >  1:3121079/1‑150 (MQ=255)
                                                                                                                   cgccgcTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGc                                  >  2:2587626/1‑150 (MQ=255)
                                                                                                                    gccgcTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCg                                 >  1:3700729/1‑150 (MQ=255)
                                                                                                                    gccgcTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCg                                 >  2:1772541/1‑150 (MQ=255)
                                                                                                                     ccgcTCCCCTTGCGGCGAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGt                                >  2:4583151/1‑150 (MQ=255)
                                                                                                                     ccgcTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGt                                <  2:1860168/150‑1 (MQ=255)
                                                                                                                          ccccTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAgg                           <  1:82296/150‑1 (MQ=255)
                                                                                                                             cTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCAt                        >  2:2974307/1‑150 (MQ=255)
                                                                                                                             cTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCAt                        >  2:1870640/1‑150 (MQ=255)
                                                                                                                              ttGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCAtt                       <  1:2779111/150‑1 (MQ=255)
                                                                                                                               tGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATtg                      <  1:4370243/150‑1 (MQ=255)
                                                                                                                                gcggcgAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATtga                     <  2:5071633/150‑1 (MQ=255)
                                                                                                                                  ggcgAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATtgatg                   >  1:114118/1‑150 (MQ=255)
                                                                                                                                   gcgAACGGATGATCAGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATtgatga                  >  1:3660204/1‑150 (MQ=255)
                                                                                                                                    cgAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGAc                 <  1:3217164/150‑1 (MQ=255)
                                                                                                                                     gAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGAcc                >  2:1202599/1‑150 (MQ=255)
                                                                                                                                     gAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGAcc                >  2:2005335/1‑150 (MQ=255)
                                                                                                                                       aCGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCg              >  1:1414258/1‑150 (MQ=255)
                                                                                                                                        cGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGt             <  1:1836034/150‑1 (MQ=255)
                                                                                                                                         ggATGATCAGCGGCATGCGGGTGATGTCGTCATACATCGCCGCCCCTTGACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGTc            <  2:4522045/150‑1 (MQ=255)
                                                                                                                                              atCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGTCCGAtt       <  2:2522273/150‑1 (MQ=255)
                                                                                                                                               tCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGTCCGAttt      <  2:307465/150‑1 (MQ=255)
                                                                                                                                                 aGCTGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGTCCGATTTgg    >  2:1547260/1‑150 (MQ=255)
                                                                                                                                                 aGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGTCCGATTTgg    >  2:4799351/1‑150 (MQ=255)
                                                                                                                                                   cGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGTCCGATTTGGtc  <  1:1368797/150‑1 (MQ=255)
                                                                                                                                                   cGGAATGCGGGTGATGTCGTCATACATCGCCGACCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGTCCGATTTGGtc  <  1:3965218/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGGCTCTTTCACGGCAAGGATATTTTCCCCCGGCAGAATCTCTGGTTTTTCAATATCTGCCAGCGCCATCATTGTCGGCAGTAAATCGATATGACTGACTGGCGTATCGACCTGTCGCCGCTCCCCTTGCGGCGAACGGATGATCAGCGGAATGCGGGTGATGTCGTCATACATCGCCGCCCCTTTACTGATCAGCTTATGTGCGCCCATCATTTCGCCGTGATCGGAGGTATAAATAACCCACGTATTTTCACGTTGCTCTGGCGTTAAGGCATTGATGACCCGTCCGATTTGGTC  >  CP009273/3850691‑3850987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A