Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F52 I0 R1 779 320.1 10410758 96.6% 10056792 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 705,588 C→A 37.1% intergenic (+16/+68) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273705,5880CA37.1% 469.7 / inf 334intergenic (+16/+68)chiQ/furchitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (107/103);  new base A (69/55);  total (176/158)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 348 total aligned reads displayed.

GTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGT  >  CP009273/705440‑705736
                                                                                                                                                    |                                                                                                                                                    
gTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAActc                                                                                                                                                      <  2:892740/149‑1 (MQ=255)
gTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAActc                                                                                                                                                      <  1:2048394/149‑1 (MQ=255)
   tGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCg                                                                                                                                                   >  1:113168/1‑149 (MQ=255)
     ccGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCt                                                                                                                                                 <  1:1481830/149‑1 (MQ=255)
       gACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCttt                                                                                                                                               <  1:1984760/149‑1 (MQ=255)
       gACCAGGAAAGTGTACGCGTACTGGATGATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCttt                                                                                                                                               <  2:3539186/149‑1 (MQ=255)
         ccAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTc                                                                                                                                             >  2:1832271/1‑149 (MQ=255)
         ccAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTc                                                                                                                                             >  2:4327569/1‑149 (MQ=255)
          cAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAACTAAGTGAGAGCTGTAACTCTCGCTTTTCt                                                                                                                                            <  2:2165893/149‑1 (MQ=255)
          cAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCt                                                                                                                                            <  1:1609022/149‑1 (MQ=255)
            ggAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTa                                                                                                                                          <  2:2262119/149‑1 (MQ=255)
            ggAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTa                                                                                                                                          <  2:1170415/149‑1 (MQ=255)
            ggAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTa                                                                                                                                          >  2:2560446/1‑149 (MQ=255)
            ggAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTa                                                                                                                                          <  1:281523/149‑1 (MQ=255)
            ggAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTa                                                                                                                                          <  2:4599921/149‑1 (MQ=255)
                aGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTc                                                                                                                                      >  1:3329837/1‑149 (MQ=255)
                 gtgtACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTcc                                                                                                                                     <  2:3070740/149‑1 (MQ=255)
                   gtACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCt                                                                                                                                   <  2:1984416/149‑1 (MQ=255)
                      cgcgTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGc                                                                                                                                >  2:2448349/1‑149 (MQ=255)
                       gcgTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCa                                                                                                                               >  1:1238083/1‑149 (MQ=255)
                       gcgTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCa                                                                                                                               <  2:83899/149‑1 (MQ=255)
                        cgTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCAt                                                                                                                              <  2:379003/149‑1 (MQ=255)
                        cgTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCCTTTCTTATTTCCCTTGCAt                                                                                                                              <  2:4502964/149‑1 (MQ=255)
                           aCTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATaaa                                                                                                                           <  1:1836474/149‑1 (MQ=255)
                              ggATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTCTTTCCCTTGCATaaaaaa                                                                                                                        >  2:3134694/1‑149 (MQ=255)
                                 ttATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGcc                                                                                                                     >  1:3660714/1‑149 (MQ=255)
                                  tATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCa                                                                                                                    >  1:2929638/1‑149 (MQ=255)
                                  tATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTACTTATTTCCCTAGCATAAAAAAGCCa                                                                                                                    >  1:327423/1‑149 (MQ=255)
                                   aTCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCaa                                                                                                                   <  2:3693975/149‑1 (MQ=255)
                                   aTCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCaa                                                                                                                   <  1:3908524/149‑1 (MQ=255)
                                      agcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAccc                                                                                                                <  1:2811081/149‑1 (MQ=255)
                                       gcagTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCg                                                                                                               >  1:3958810/1‑149 (MQ=255)
                                         agTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCa                                                                                                             <  2:3193598/149‑1 (MQ=255)
                                             tCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGtt                                                                                                         <  1:2038075/149‑1 (MQ=255)
                                             tCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGtt                                                                                                         <  2:4805718/149‑1 (MQ=255)
                                                gCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGc                                                                                                      <  1:640873/149‑1 (MQ=255)
                                                 cGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATACCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCt                                                                                                     <  1:1004382/149‑1 (MQ=255)
                                                     cAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                                 <  1:4839399/149‑1 (MQ=255)
                                                     cAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                                 <  2:1641412/149‑1 (MQ=255)
                                                      aaCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct                                                                                                >  2:3860682/1‑149 (MQ=255)
                                                       aCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtctc                                                                                               >  1:3416977/1‑149 (MQ=255)
                                                       aCGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtctc                                                                                               <  1:1087189/149‑1 (MQ=255)
                                                         gCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGt                                                                                             <  1:5110358/149‑1 (MQ=255)
                                                          cAAACCGGAAACGATCAGGCGGAGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGtt                                                                                            <  2:2710507/149‑1 (MQ=255)
                                                            aaCCTGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCa                                                                                          <  2:3050035/149‑1 (MQ=255)
                                                               cGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggc                                                                                       <  2:1893273/149‑1 (MQ=255)
                                                               cGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggc                                                                                       >  2:917059/1‑149 (MQ=255)
                                                                 gAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctg                                                                                     >  1:2548575/1‑149 (MQ=255)
                                                                   aaTGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctggc                                                                                   <  1:4907109/149‑1 (MQ=255)
                                                                    aTGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctggct                                                                                  >  1:4147413/1‑149 (MQ=255)
                                                                       aTCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtttt                                                                                                  >  2:533448/1‑130 (MQ=255)
                                                                         cAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGATTTTCTCGTTCAGGCTGGCTTAttt                                                                             >  2:4754338/1‑149 (MQ=255)
                                                                             cGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCt                                                                         <  1:2284130/149‑1 (MQ=255)
                                                                              ggTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCtt                                                                        <  1:4566198/149‑1 (MQ=255)
                                                                               gTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTc                                                                       <  2:2819690/149‑1 (MQ=255)
                                                                               gTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTc                                                                       >  1:1436453/1‑149 (MQ=255)
                                                                                   aaGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgc                                                                   >  2:573079/1‑149 (MQ=255)
                                                                                       cGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCAt                                                               <  2:5011419/149‑1 (MQ=255)
                                                                                        gATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCGTTCGTGCGCATg                                                              <  1:1314820/149‑1 (MQ=255)
                                                                                         aTTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGt                                                             <  1:4431140/149‑1 (MQ=255)
                                                                                         aTTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGt                                                             >  2:1538566/1‑149 (MQ=255)
                                                                                         aTTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGt                                                             <  1:5165870/149‑1 (MQ=255)
                                                                                          ttGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGtt                                                            >  1:561262/1‑149 (MQ=255)
                                                                                            gtgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCa                                                          >  2:721511/1‑149 (MQ=255)
                                                                                            gtgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCa                                                          <  1:2989113/149‑1 (MQ=255)
                                                                                               aTAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCt                                                       >  1:683168/1‑149 (MQ=255)
                                                                                                tAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCtt                                                      >  1:3553764/1‑149 (MQ=255)
                                                                                                  aaGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcg                                                    >  1:4131189/1‑149 (MQ=255)
                                                                                                   aGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgc                                                   <  1:2457116/149‑1 (MQ=255)
                                                                                                   aGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgc                                                   <  1:2457360/149‑1 (MQ=255)
                                                                                                     tCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCgg                                                 >  2:1411001/1‑149 (MQ=255)
                                                                                                       tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCa                                               >  1:3024659/1‑149 (MQ=255)
                                                                                                        ggCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCaa                                              >  2:2329749/1‑149 (MQ=255)
                                                                                                         gCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAAt                                             >  1:967828/1‑149 (MQ=255)
                                                                                                         gCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAAt                                             >  1:967932/1‑149 (MQ=255)
                                                                                                           aGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCg                                           >  1:3738672/1‑149 (MQ=255)
                                                                                                               aaTACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCtt                                       >  2:4105826/1‑149 (MQ=255)
                                                                                                                    gCAGTAATAACAAATACGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGca                                  <  1:2230121/149‑1 (MQ=255)
                                                                                                                    gCAGTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGca                                  >  2:2067526/1‑149 (MQ=255)
                                                                                                                       gTAATAACAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAg                               >  1:4070029/1‑149 (MQ=255)
                                                                                                                        taataaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGt                              <  1:849052/149‑1 (MQ=255)
                                                                                                                        taataaCAAAGAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGt                              <  1:3150532/149‑1 (MQ=255)
                                                                                                                         aataaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTg                             <  1:3511865/149‑1 (MQ=255)
                                                                                                                         aataaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTg                             <  2:1235453/149‑1 (MQ=255)
                                                                                                                           taaCAAATAAGTGAGAGCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAc                           <  2:1086895/149‑1 (MQ=255)
                                                                                                                            aaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAcc                          >  2:4931423/1‑149 (MQ=255)
                                                                                                                                 aTAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaaa                     >  2:4603860/1‑149 (MQ=255)
                                                                                                                                  tAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGACAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTTCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaatg                    >  2:4863840/1‑147 (MQ=255)
                                                                                                                                   aaGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGa                   <  1:620975/149‑1 (MQ=255)
                                                                                                                                     gTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATa                 <  1:1712788/149‑1 (MQ=255)
                                                                                                                                     gTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATa                 >  2:3231989/1‑149 (MQ=255)
                                                                                                                                     gTGAGAGCTGTAACTCTCGCTTTTCTTATGTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATa                 <  2:112936/149‑1 (MQ=255)
                                                                                                                                          agCTGTAACTATCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGAc            <  2:3959425/149‑1 (MQ=255)
                                                                                                                                           gCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACt           >  2:5106905/1‑149 (MQ=255)
                                                                                                                                           gCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACt           >  2:3628215/1‑149 (MQ=255)
                                                                                                                                              gTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACtgtg        >  2:1210929/1‑149 (MQ=255)
                                                                                                                                              gTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACtgtg        <  1:3090731/149‑1 (MQ=255)
                                                                                                                                               tAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTgg       >  1:5031789/1‑149 (MQ=255)
                                                                                                                                                aaCTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGt      <  2:4147413/149‑1 (MQ=255)
                                                                                                                                                   tctcGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAg   <  1:4329030/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTTTGCCGACCAGGAAAGTGTACGCGTACTGGATTATCAGCAGTGTCTGCGTGCAACGCAAACCGGAAATGATCAGGCGGTGAAAGCCGATTGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGACTGTGGTTAGT  >  CP009273/705440‑705736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A