Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 186,279 A→T 11.8% noncoding (81/136 nt) tff → novel sRNA, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273186,2790AT11.8% 574.1 / 58.2 194noncoding (81/136 nt)tffnovel sRNA, function unknown
Reads supporting (aligned to +/- strand):  ref base A (79/92);  new base T (15/8);  total (94/100)
Fisher's exact test for biased strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 205 total aligned reads displayed.

CGTCTGATGCTGTACACAGCGCCAACAATTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTA  >  CP009273/186132‑186425
                                                                                                                                                   |                                                                                                                                                  
cGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGca                                                                                                                                                    <  1:396865/148‑1 (MQ=255)
cGTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGca                                                                                                                                                    >  1:771411/1‑148 (MQ=255)
 gTCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGcac                                                                                                                                                   >  2:1091369/1‑148 (MQ=255)
  tCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGcaca                                                                                                                                                  >  2:2460164/1‑148 (MQ=255)
  tCTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGcaca                                                                                                                                                  >  1:2191480/1‑148 (MQ=255)
   cTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACat                                                                                                                                                 <  1:734837/148‑1 (MQ=255)
   cTGATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACat                                                                                                                                                 <  1:1744718/148‑1 (MQ=255)
     gATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACatat                                                                                                                                               >  1:531478/1‑148 (MQ=255)
     gATGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACatat                                                                                                                                               >  2:3150837/1‑148 (MQ=255)
      aTGCTGTACACAGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATAtt                                                                                                                                              <  1:998991/148‑1 (MQ=255)
              cacaGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACACTCTCACTTTGTGTAACAACACACACGTATCGGCACATATTACGGGGTg                                                                                                                                      >  1:2832518/1‑148 (MQ=255)
              cacaGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTg                                                                                                                                      <  1:2913717/148‑1 (MQ=255)
              cacaGCGCCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTg                                                                                                                                      >  1:2175280/1‑148 (MQ=255)
                 aGCGCCAACAGTTATTGGTGTCCACTACGTATTTGTGTTATAAAGCGCGCCTGACTTCCGATCAATATCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAAAACACACGAATCGGCACATATACAGTGGTGccc                                                                                                                                   >  2:2719792/1‑148 (MQ=255)
                   cgcCAACAGTTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCtt                                                                                                                                 >  1:885044/1‑148 (MQ=255)
                         cagttattGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGt                                                                                                                           >  1:2074044/4‑148 (MQ=255)
                         cagttattGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGt                                                                                                                           <  1:2332527/145‑1 (MQ=255)
                             tattGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGt                                                                                                                       <  2:2393514/148‑1 (MQ=255)
                             tattGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGt                                                                                                                       <  2:2546051/148‑1 (MQ=255)
                               ttGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCTCATATTCCGGGGTGCCCTTTGGGGTCGGTaa                                                                                                                     >  2:2137358/1‑148 (MQ=255)
                               ttGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTaa                                                                                                                     <  2:2191468/148‑1 (MQ=255)
                                tGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCTCATATTCCGGGGTGCCCTTTGGGGTCGGTAat                                                                                                                    >  1:1000197/1‑148 (MQ=255)
                                tGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAat                                                                                                                    <  2:2967861/148‑1 (MQ=255)
                                tGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAat                                                                                                                    >  2:46150/1‑148 (MQ=255)
                                tGGTGTCCACGAAGTATTTGTGGTATAAAGCGCGGCGGACTTCGGATCCTTTTCGTTTACACATACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAat                                                                                                                    <  1:1689635/148‑1 (MQ=255)
                                   tgtCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACTATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATg                                                                                                                 <  2:2204711/148‑1 (MQ=255)
                                   tgtCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATg                                                                                                                 <  2:2613946/148‑1 (MQ=255)
                                        acgacgTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATa                                                                                                            <  1:2725376/148‑1 (MQ=255)
                                          gacgTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACg                                                                                                          <  2:2118285/148‑1 (MQ=255)
                                           acgTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGt                                                                                                         >  2:1907218/1‑148 (MQ=255)
                                            cgTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTg                                                                                                        >  2:1342275/1‑148 (MQ=255)
                                               aTTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAg                                                                                                     <  1:1627452/148‑1 (MQ=255)
                                               aTTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAg                                                                                                     >  1:2936780/1‑148 (MQ=255)
                                               aTTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAg                                                                                                     >  1:134410/1‑148 (MQ=255)
                                               aTTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAg                                                                                                     >  2:2061142/1‑148 (MQ=255)
                                                tttGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAgg                                                                                                    >  1:2750241/1‑148 (MQ=255)
                                                 ttGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGc                                                                                                   >  1:2745076/1‑148 (MQ=255)
                                                 ttGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGc                                                                                                   >  1:1940677/1‑148 (MQ=255)
                                                 ttGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGc                                                                                                   >  2:2257149/1‑148 (MQ=255)
                                                     ggTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATaa                                                                                               <  2:3071515/148‑1 (MQ=255)
                                                        ataAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAAccc                                                                                            <  2:2647679/148‑1 (MQ=255)
                                                        ataAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAAccc                                                                                            <  2:771411/148‑1 (MQ=255)
                                                        ataAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAAccc                                                                                            <  2:537987/148‑1 (MQ=255)
                                                         taAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAAcccc                                                                                           >  1:2700937/1‑148 (MQ=255)
                                                          aaaGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCa                                                                                          >  1:1290699/1‑148 (MQ=255)
                                                            aGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCacc                                                                                        >  2:2798100/1‑146 (MQ=255)
                                                            aGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAAc                                                                                        >  2:1430464/1‑148 (MQ=255)
                                                              cgcgcCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACtt                                                                                      <  1:182496/148‑1 (MQ=255)
                                                               gcgcCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACttt                                                                                     <  1:2678004/148‑1 (MQ=255)
                                                               gcgcCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACttt                                                                                     <  1:2345130/148‑1 (MQ=255)
                                                                cgcCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACtttt                                                                                    <  1:2245707/148‑1 (MQ=255)
                                                                 gcCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTta                                                                                   <  1:2362695/148‑1 (MQ=255)
                                                                 gcCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTta                                                                                   <  1:1469000/148‑1 (MQ=255)
                                                                  ccGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTtat                                                                                  <  1:2491512/148‑1 (MQ=255)
                                                                  ccGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTtat                                                                                  <  1:137020/148‑1 (MQ=255)
                                                                   cGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTtata                                                                                 <  1:633459/148‑1 (MQ=255)
                                                                        ttCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCTCATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATagag                                                                            >  2:900774/1‑148 (MQ=255)
                                                                        ttCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATagag                                                                            >  1:1229012/1‑148 (MQ=255)
                                                                          ccGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCTCATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGt                                                                          <  1:2800449/148‑1 (MQ=255)
                                                                          ccGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGt                                                                          <  1:1990042/148‑1 (MQ=255)
                                                                          ccGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGt                                                                          <  1:46150/148‑1 (MQ=255)
                                                                            gATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGttt                                                                        <  1:2125995/148‑1 (MQ=255)
                                                                            gATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGttt                                                                        <  2:2582433/148‑1 (MQ=255)
                                                                              tCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTa                                                                      >  2:975633/1‑148 (MQ=255)
                                                                                cATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAAt                                                                    <  2:1243524/148‑1 (MQ=255)
                                                                                cATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAAt                                                                    <  2:2891707/148‑1 (MQ=255)
                                                                                 aTTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATc                                                                   <  2:2074044/148‑1 (MQ=255)
                                                                                 aTTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATc                                                                   <  2:2832957/148‑1 (MQ=255)
                                                                                  tttCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCTCATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTGTTATATAGAGGTTTTAATCa                                                                  >  2:1011118/1‑148 (MQ=255)
                                                                                  tttCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCa                                                                  <  2:1902999/148‑1 (MQ=255)
                                                                                  tttCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCa                                                                  <  2:689846/148‑1 (MQ=255)
                                                                                          acacAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt                                                          >  2:1808213/1‑148 (MQ=255)
                                                                                           cacaGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTg                                                         >  1:981251/1‑148 (MQ=255)
                                                                                            acaGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGt                                                        <  2:1684034/148‑1 (MQ=255)
                                                                                             caGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGtt                                                       <  1:675341/148‑1 (MQ=255)
                                                                                                  tGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCAt                                                  >  2:685265/1‑148 (MQ=255)
                                                                                                    gACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATgc                                                >  2:2032861/1‑148 (MQ=255)
                                                                                                    gACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATgc                                                >  2:156115/1‑148 (MQ=255)
                                                                                                      cGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATgcgc                                              <  2:2214280/148‑1 (MQ=255)
                                                                                                        gAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGa                                            >  2:1356379/1‑148 (MQ=255)
                                                                                                          aGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACa                                          <  1:1314817/148‑1 (MQ=255)
                                                                                                           gCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACAt                                         >  2:452359/1‑148 (MQ=255)
                                                                                                            cGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGCAATATGGGATACGTGGAGGCATAACCCCAACTGTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATg                                        >  1:2293539/1‑148 (MQ=255)
                                                                                                               cAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTc                                     >  1:1509913/1‑148 (MQ=255)
                                                                                                                     cACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCt                               >  2:1181297/1‑148 (MQ=255)
                                                                                                                       cTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTgg                             <  1:1711186/148‑1 (MQ=255)
                                                                                                                           gtgtAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt                         <  1:3150837/148‑1 (MQ=255)
                                                                                                                           gtgtAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt                         <  2:2495372/148‑1 (MQ=255)
                                                                                                                           gtgtAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt                         <  2:837022/148‑1 (MQ=255)
                                                                                                                             gtAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCa                       <  1:158542/148‑1 (MQ=255)
                                                                                                                                acaacaCACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACtt                    >  2:332146/1‑148 (MQ=255)
                                                                                                                                       acacGTATCGGCTCATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCAc             >  1:255690/1‑148 (MQ=255)
                                                                                                                                       acacGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCAc             <  1:2889617/148‑1 (MQ=255)
                                                                                                                                        cacGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCAcc            >  2:409893/1‑148 (MQ=255)
                                                                                                                                             aTCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGAcc       >  2:370434/1‑148 (MQ=255)
                                                                                                                                             aTCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGAcc       >  2:2067795/1‑148 (MQ=255)
                                                                                                                                             aTCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGAcc       >  2:1632102/1‑148 (MQ=255)
                                                                                                                                                  cacaTATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTa  >  1:160564/1‑148 (MQ=255)
                                                                                                                                                  cacaTATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTa  <  1:3028884/148‑1 (MQ=255)
                                                                                                                                                  cacaTATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTa  <  1:417823/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTCTGATGCTGTACACAGCGCCAACAATTATTGGTGTCCACGACGTATTTGTGGTATAAAGCGCGCCGGACTTCCGATCCATTTCGTATACACAGACTGGACGGAAGCGACAATCTCACTTTGTGTAACAACACACACGTATCGGCACATATTCCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTA  >  CP009273/186132‑186425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A