Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 214,084 G→T 100% G393G (GGC→GGA)  proS ← prolyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273214,0840GT95.5% 469.1 / 13.0 155G393G (GGC→GGA) proSprolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (3/4);  new base T (74/74);  total (77/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 158 total aligned reads displayed.

CCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAA  >  CP009273/213939‑214229
                                                                                                                                                 |                                                                                                                                                 
ccATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCtcc                                                                                                                                                 <  1:2659773/148‑1 (MQ=255)
      tCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGccac                                                                                                                                           <  2:1845827/148‑1 (MQ=255)
      tCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGccac                                                                                                                                           >  1:687644/1‑148 (MQ=255)
      tCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGccac                                                                                                                                           >  1:398822/1‑148 (MQ=255)
        aGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGccacca                                                                                                                                         <  2:2967413/148‑1 (MQ=255)
          gATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCATCACg                                                                                                                                       <  1:2296766/148‑1 (MQ=255)
          gATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCATCACg                                                                                                                                       <  1:2296788/148‑1 (MQ=255)
          gATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACg                                                                                                                                       <  1:1003469/148‑1 (MQ=255)
          gATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACg                                                                                                                                       >  1:2112155/1‑148 (MQ=255)
          gATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACg                                                                                                                                       <  1:1918457/148‑1 (MQ=255)
          gATTTGGTTACGGCCATCGTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACg                                                                                                                                       <  2:2516468/148‑1 (MQ=255)
            tttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGtt                                                                                                                                     >  1:3012997/1‑148 (MQ=255)
            tttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGtt                                                                                                                                     >  1:3012957/1‑148 (MQ=255)
            tttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGtt                                                                                                                                     >  1:2486879/1‑148 (MQ=255)
            tttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGtt                                                                                                                                     >  1:790703/1‑148 (MQ=255)
             ttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTa                                                                                                                                    <  1:3287168/148‑1 (MQ=255)
                gTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACgg                                                                                                                                 <  2:2123017/148‑1 (MQ=255)
                    cGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGatat                                                                                                                             <  1:1720808/148‑1 (MQ=255)
                     ggCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATc                                                                                                                            <  1:3256458/148‑1 (MQ=255)
                       ccATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTg                                                                                                                          >  1:461705/1‑148 (MQ=255)
                       ccATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACACTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTg                                                                                                                          >  1:2601481/1‑148 (MQ=255)
                        cATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGc                                                                                                                         >  1:862404/1‑148 (MQ=255)
                        cATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGc                                                                                                                         >  1:259761/1‑148 (MQ=255)
                         aTCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCa                                                                                                                        >  2:3198222/1‑148 (MQ=255)
                          tCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCaa                                                                                                                       >  2:2005774/1‑148 (MQ=255)
                           cTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAAc                                                                                                                      <  1:2096981/148‑1 (MQ=255)
                           cTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAAc                                                                                                                      >  1:755387/1‑148 (MQ=255)
                             tCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACtt                                                                                                                    <  1:2895296/148‑1 (MQ=255)
                               aCCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTcc                                                                                                                  >  1:3160193/1‑148 (MQ=255)
                                 ccTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCgg                                                                                                                >  1:816729/1‑148 (MQ=255)
                                   tGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCgggg                                                                                                              <  2:518520/148‑1 (MQ=255)
                                   tGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCgggg                                                                                                              <  2:2732147/148‑1 (MQ=255)
                                         gAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGa                                                                                                        >  1:1283071/1‑148 (MQ=255)
                                              tttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcg                                                                                                   <  1:2307378/148‑1 (MQ=255)
                                               ttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgc                                                                                                  >  2:2425600/1‑148 (MQ=255)
                                                tCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgcg                                                                                                 >  1:2363110/1‑148 (MQ=255)
                                                tCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgcg                                                                                                 >  1:2363107/1‑148 (MQ=255)
                                                 cAGTGCTTCGGAGTACTTGGTTCCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGa                                                                                                <  2:602602/148‑1 (MQ=255)
                                                 cAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGa                                                                                                <  2:2264430/148‑1 (MQ=255)
                                                 cAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGa                                                                                                >  2:1471816/1‑148 (MQ=255)
                                                 cAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGa                                                                                                >  2:1470074/1‑148 (MQ=255)
                                                     gCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATccc                                                                                            >  1:2871187/1‑148 (MQ=255)
                                                          ggAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTg                                                                                       <  1:2770859/148‑1 (MQ=255)
                                                           gAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGa                                                                                      <  2:2376327/148‑1 (MQ=255)
                                                           gAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGa                                                                                      <  2:2824568/148‑1 (MQ=255)
                                                                 ttGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGa                                                                                <  1:3179518/148‑1 (MQ=255)
                                                                  tGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGaa                                                                               <  1:2143620/148‑1 (MQ=255)
                                                                  tGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGaa                                                                               >  2:1825097/1‑148 (MQ=255)
                                                                     tACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagta                                                                            <  1:1459643/148‑1 (MQ=255)
                                                                       cccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagtagt                                                                          <  2:2112153/148‑1 (MQ=255)
                                                                       cccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagtagt                                                                          <  1:900621/148‑1 (MQ=255)
                                                                        ccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtg                                                                         <  1:3150198/148‑1 (MQ=255)
                                                                        ccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtg                                                                         <  1:3148269/148‑1 (MQ=255)
                                                                        ccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtg                                                                         <  1:1279554/148‑1 (MQ=255)
                                                                          aGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGtt                                                                       >  2:3061936/1‑148 (MQ=255)
                                                                           gCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGttt                                                                      >  2:734653/1‑148 (MQ=255)
                                                                               gAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCa                                                                  >  2:2132778/1‑148 (MQ=255)
                                                                               gAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCa                                                                  >  2:2038297/1‑148 (MQ=255)
                                                                               gAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCa                                                                  >  2:278149/1‑148 (MQ=255)
                                                                                aaGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCAt                                                                 <  1:278149/148‑1 (MQ=255)
                                                                                aaGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCAt                                                                 <  1:2038294/148‑1 (MQ=255)
                                                                                  gATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCg                                                               <  1:2714205/148‑1 (MQ=255)
                                                                                   aTGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGa                                                              <  2:1182083/148‑1 (MQ=255)
                                                                                   aTGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGa                                                              <  1:1557874/148‑1 (MQ=255)
                                                                                    tgtgACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGAt                                                             <  1:2970825/148‑1 (MQ=255)
                                                                                      tgACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGt                                                           <  2:1271372/148‑1 (MQ=255)
                                                                                      tgACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGt                                                           <  2:1399063/148‑1 (MQ=255)
                                                                                        aCCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTa                                                         >  1:1175952/1‑148 (MQ=255)
                                                                                        aCCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTa                                                         >  1:1205358/1‑148 (MQ=255)
                                                                                           aaCTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCa                                                      <  1:1563760/148‑1 (MQ=255)
                                                                                             cTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCAcc                                                    >  2:722085/1‑148 (MQ=255)
                                                                                             cTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCAcc                                                    >  1:957648/1‑148 (MQ=255)
                                                                                                  aTACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACcagcag                                               <  1:1859076/148‑1 (MQ=255)
                                                                                                   tACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCagcagc                                              >  1:3280475/1‑148 (MQ=255)
                                                                                                       aCGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGaaa                                          <  1:221762/148‑1 (MQ=255)
                                                                                                       aCGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGaaa                                          >  1:25664/1‑148 (MQ=255)
                                                                                                           ttGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCAc                                      >  2:328785/1‑148 (MQ=255)
                                                                                                            tGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACt                                     <  2:140728/148‑1 (MQ=255)
                                                                                                            tGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACt                                     <  2:1813257/148‑1 (MQ=255)
                                                                                                              aTCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCa                                   >  2:1352225/1‑148 (MQ=255)
                                                                                                              aTCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCa                                   >  1:843878/1‑148 (MQ=255)
                                                                                                                cagcagCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACCGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATc                                 >  1:1340442/1‑148 (MQ=255)
                                                                                                                  gcagcCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcgc                               <  2:281684/148‑1 (MQ=255)
                                                                                                                     gcCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcgccgc                            >  2:1223868/1‑148 (MQ=255)
                                                                                                                      ccTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCCTCGATGTTAGCACCAGCAGCGAAAGCACTCATCGCCGCa                           >  2:2413873/1‑148 (MQ=255)
                                                                                                                      ccTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCa                           >  1:270361/1‑148 (MQ=255)
                                                                                                                      ccTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCa                           >  1:565691/1‑148 (MQ=255)
                                                                                                                      ccTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCa                           >  1:2050565/1‑148 (MQ=255)
                                                                                                                       cTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCaa                          <  1:1684014/148‑1 (MQ=255)
                                                                                                                         aCCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacg                        >  2:1557500/1‑148 (MQ=255)
                                                                                                                              ggCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtacg                   >  1:448490/1‑148 (MQ=255)
                                                                                                                              ggCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtacg                   <  1:1406030/148‑1 (MQ=255)
                                                                                                                               gCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAccggtacgg                  >  2:1639340/1‑148 (MQ=255)
                                                                                                                                  aTCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCa               <  1:722085/148‑1 (MQ=255)
                                                                                                                                   tCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCaa              <  1:2188777/148‑1 (MQ=255)
                                                                                                                                   tCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACGTCCGGGGTCGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCac              >  2:1873182/1‑147 (MQ=255)
                                                                                                                                    ccGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAt             >  1:2041806/1‑148 (MQ=255)
                                                                                                                                         cTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcacca        >  1:1444021/1‑148 (MQ=255)
                                                                                                                                         cTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcacca        >  2:3102324/1‑148 (MQ=255)
                                                                                                                                              aTCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGa   <  1:499347/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAA  >  CP009273/213939‑214229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATC  >  CP009273/213936‑214231
                                                                                                                                                    |                                                                                                                                                   
AACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTC                                                                                                                                                    >  SRR19973070.283601/1‑150 (MQ=60)
AACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTC                                                                                                                                                    >  SRR19973070.283536/1‑150 (MQ=60)
   CCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAG                                                                                                                                                 <  SRR19973070.2677071/150‑1 (MQ=60)
       CGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCAC                                                                                                                                             >  SRR19973070.692549/1‑150 (MQ=60)
       CGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCAC                                                                                                                                             <  SRR19973070.441126/150‑1 (MQ=60)
       CGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCAC                                                                                                                                             >  SRR19973070.401753/1‑150 (MQ=60)
           AGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACG                                                                                                                                         >  SRR19973070.2126315/1‑150 (MQ=60)
           AGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACG                                                                                                                                         >  SRR19973070.2126317/1‑150 (MQ=60)
             GATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCATCACGTT                                                                                                                                       <  SRR19973070.2312069/150‑1 (MQ=60)
             GATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCATCACGTT                                                                                                                                       <  SRR19973070.2312047/150‑1 (MQ=60)
             GATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTT                                                                                                                                       <  SRR19973070.1931395/150‑1 (MQ=60)
             GATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTT                                                                                                                                       >  SRR19973070.796183/1‑150 (MQ=60)
             GATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTT                                                                                                                                       >  SRR19973070.2503037/1‑150 (MQ=60)
             GATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTT                                                                                                                                       >  SRR19973070.3032179/1‑150 (MQ=60)
             GATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTT                                                                                                                                       >  SRR19973070.3032219/1‑150 (MQ=60)
             GATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTT                                                                                                                                       <  SRR19973070.1010615/150‑1 (MQ=60)
              ATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTA                                                                                                                                      <  SRR19973070.306357/150‑1 (MQ=60)
                TTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACG                                                                                                                                    <  SRR19973070.3308270/150‑1 (MQ=60)
                   GTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGAT                                                                                                                                 <  SRR19973070.61579/150‑1 (MQ=60)
                       CGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCT                                                                                                                             <  SRR19973070.1732717/150‑1 (MQ=60)
                        GGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTG                                                                                                                            >  SRR19973070.464998/1‑150 (MQ=60)
                        GGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTG                                                                                                                            <  SRR19973070.3277432/150‑1 (MQ=60)
                        GGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTG                                                                                                                            >  SRR19973070.465703/1‑150 (MQ=60)
                        GGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACACTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTG                                                                                                                            >  SRR19973070.2618465/1‑150 (MQ=60)
                         GCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGC                                                                                                                           >  SRR19973070.261501/1‑150 (MQ=60)
                         GCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGC                                                                                                                           >  SRR19973070.868401/1‑150 (MQ=60)
                            ATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAAC                                                                                                                        >  SRR19973070.760725/1‑150 (MQ=60)
                            ATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAAC                                                                                                                        >  SRR19973070.760715/1‑150 (MQ=60)
                            ATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAAC                                                                                                                        >  SRR19973070.1558259/1‑150 (MQ=60)
                              CTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTT                                                                                                                      <  SRR19973070.2111069/150‑1 (MQ=60)
                              CTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTT                                                                                                                      <  SRR19973070.3300964/150‑1 (MQ=60)
                               TTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTC                                                                                                                     <  SRR19973070.2686392/150‑1 (MQ=60)
                                TCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCC                                                                                                                    >  SRR19973070.3180643/1‑150 (MQ=60)
                                TCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCC                                                                                                                    >  SRR19973070.3180673/1‑150 (MQ=60)
                                TCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCC                                                                                                                    <  SRR19973070.90242/150‑1 (MQ=60)
                                TCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCC                                                                                                                    <  SRR19973070.2914025/150‑1 (MQ=60)
                                  ACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGG                                                                                                                  >  SRR19973070.822310/1‑150 (MQ=60)
                                  ACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGG                                                                                                                  >  SRR19973070.2490897/1‑150 (MQ=60)
                                          CGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGA                                                                                                          >  SRR19973070.1292030/1‑150 (MQ=60)
                                          CGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGA                                                                                                          >  SRR19973070.1292005/1‑150 (MQ=60)
                                             AGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACAT                                                                                                       >  SRR19973070.1812477/1‑150 (MQ=60)
                                               GCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCG                                                                                                     <  SRR19973070.2603554/150‑1 (MQ=60)
                                                 TTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCG                                                                                                   >  SRR19973070.2378742/1‑150 (MQ=60)
                                                 TTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCG                                                                                                   >  SRR19973070.2378745/1‑150 (MQ=60)
                                                 TTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCG                                                                                                   <  SRR19973070.2322717/150‑1 (MQ=60)
                                                 TTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCG                                                                                                   <  SRR19973070.2322515/150‑1 (MQ=60)
                                                      GTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCC                                                                                              >  SRR19973070.2889788/1‑150 (MQ=60)
                                                      GTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCC                                                                                              >  SRR19973070.2889784/1‑150 (MQ=60)
                                                           TCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTG                                                                                         <  SRR19973070.2658479/150‑1 (MQ=60)
                                                             GGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGAT                                                                                       <  SRR19973070.1687104/150‑1 (MQ=60)
                                                             GGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGAT                                                                                       <  SRR19973070.2788590/150‑1 (MQ=60)
                                                                    TTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAG                                                                                <  SRR19973070.3200099/150‑1 (MQ=60)
                                                                     TGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGT                                                                               <  SRR19973070.2157911/150‑1 (MQ=60)
                                                                        TACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGT                                                                            <  SRR19973070.1469824/150‑1 (MQ=60)
                                                                         ACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTG                                                                           >  SRR19973070.2865361/1‑150 (MQ=60)
                                                                         ACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTG                                                                           <  SRR19973070.2355154/150‑1 (MQ=60)
                                                                          CCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGT                                                                          <  SRR19973070.907002/150‑1 (MQ=60)
                                                                           CCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTT                                                                         <  SRR19973070.3168613/150‑1 (MQ=60)
                                                                           CCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTT                                                                         <  SRR19973070.3170556/150‑1 (MQ=60)
                                                                           CCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTT                                                                         <  SRR19973070.1288492/150‑1 (MQ=60)
                                                                                 GGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCAT                                                                   >  SRR19973070.2078784/1‑150 (MQ=60)
                                                                                   AAGATGTGACCAACTTCGATACCACGTTTTATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCG                                                                 <  SRR19973070.2052088/150‑1 (MQ=60)
                                                                                   AAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCG                                                                 <  SRR19973070.2052085/150‑1 (MQ=60)
                                                                                   AAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCG                                                                 <  SRR19973070.279952/150‑1 (MQ=60)
                                                                                     GATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGAT                                                               <  SRR19973070.2731772/150‑1 (MQ=60)
                                                                                     GATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGAT                                                               <  SRR19973070.2731740/150‑1 (MQ=60)
                                                                                      ATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATG                                                              <  SRR19973070.1568836/150‑1 (MQ=60)
                                                                                       TGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGT                                                             <  SRR19973070.2989910/150‑1 (MQ=60)
                                                                                       TGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGT                                                             <  SRR19973070.2990898/150‑1 (MQ=60)
                                                                                       TGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGT                                                             >  SRR19973070.826404/1‑150 (MQ=60)
                                                                                         TGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTA                                                           >  SRR19973070.1184192/1‑150 (MQ=60)
                                                                                         TGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTA                                                           >  SRR19973070.1213128/1‑150 (MQ=60)
                                                                                         TGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTA                                                           >  SRR19973070.1213820/1‑150 (MQ=60)
                                                                                              AACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACC                                                      >  SRR19973070.964479/1‑150 (MQ=60)
                                                                                              AACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACC                                                      <  SRR19973070.1574768/150‑1 (MQ=60)
                                                                                                    GATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGC                                                >  SRR19973070.3301548/1‑150 (MQ=60)
                                                                                                     ATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCG                                               <  SRR19973070.1871798/150‑1 (MQ=60)
                                                                                                        CCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAA                                            >  SRR19973070.2196939/1‑150 (MQ=60)
                                                                                                        CCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAA                                            >  SRR19973070.26017/1‑150 (MQ=60)
                                                                                                          ACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATC                                          <  SRR19973070.223367/150‑1 (MQ=60)
                                                                                                           CGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCA                                         >  SRR19973070.1873501/1‑150 (MQ=60)
                                                                                                            GTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCAC                                        >  SRR19973070.1411794/1‑150 (MQ=60)
                                                                                                               TGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCA                                     >  SRR19973070.849612/1‑150 (MQ=60)
                                                                                                                 ATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACCGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATC                                   >  SRR19973070.1349652/1‑150 (MQ=60)
                                                                                                                       AGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCA                             >  SRR19973070.272137/1‑150 (MQ=60)
                                                                                                                       AGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCA                             >  SRR19973070.2064435/1‑150 (MQ=60)
                                                                                                                       AGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCA                             >  SRR19973070.569485/1‑150 (MQ=60)
                                                                                                                          CTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACG                          <  SRR19973070.3171718/150‑1 (MQ=60)
                                                                                                                          CTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACG                          <  SRR19973070.1695565/150‑1 (MQ=60)
                                                                                                                               CTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACG                     >  SRR19973070.451727/1‑150 (MQ=60)
                                                                                                                                 GGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGT                   <  SRR19973070.1415576/150‑1 (MQ=60)
                                                                                                                                  GCCATCCGGGCTTGGATCTCCAGCCACCACTTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTC                  <  SRR19973070.331191/150‑1 (MQ=60)
                                                                                                                                     ATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAT               <  SRR19973070.731501/150‑1 (MQ=60)
                                                                                                                                     ATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAT               <  SRR19973070.727225/150‑1 (MQ=60)
                                                                                                                                     ATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAT               >  SRR19973070.2055616/1‑150 (MQ=60)
                                                                                                                                      TCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATC              <  SRR19973070.2203236/150‑1 (MQ=60)
                                                                                                                                          GGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCA          >  SRR19973070.1453968/1‑150 (MQ=60)
                                                                                                                                          GGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCA          >  SRR19973070.1454047/1‑150 (MQ=60)
                                                                                                                                               GGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGA     >  SRR19973070.2235433/1‑150 (MQ=60)
                                                                                                                                                 ATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAAT   <  SRR19973070.502822/150‑1 (MQ=60)
                                                                                                                                                  TCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATC  <  SRR19973070.802261/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
AACCCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATC  >  CP009273/213936‑214231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑