Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 214,084 G→T 100% G393G (GGC→GGA)  proS ← prolyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273214,0840GT97.3% 697.3 / 8.8 224G393G (GGC→GGA) proSprolyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (1/5);  new base T (97/121);  total (98/126)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 229 total aligned reads displayed.

CCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGA  >  CP009273/213939‑214228
                                                                                                                                                 |                                                                                                                                                
ccATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTCTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCtcc                                                                                                                                                >  2:592291/1‑148 (MQ=255)
    cGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCtccagcc                                                                                                                                            <  2:3431373/148‑1 (MQ=255)
       cAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGccacc                                                                                                                                         <  1:3332095/148‑1 (MQ=255)
         ggATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCcaccac                                                                                                                                       >  1:1667083/1‑148 (MQ=255)
           aTTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGt                                                                                                                                     >  2:2167836/1‑148 (MQ=255)
             ttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTa                                                                                                                                   <  1:2576811/148‑1 (MQ=255)
             ttGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTa                                                                                                                                   >  1:233331/1‑148 (MQ=255)
                 ttACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGa                                                                                                                               >  2:3274305/1‑148 (MQ=255)
                  tACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGat                                                                                                                              >  2:869038/1‑148 (MQ=255)
                  tACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGat                                                                                                                              >  2:1522893/1‑148 (MQ=255)
                    cGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGatat                                                                                                                            <  1:1409356/148‑1 (MQ=255)
                     ggCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATc                                                                                                                           <  2:3243293/148‑1 (MQ=255)
                       ccATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTg                                                                                                                         >  1:844478/1‑148 (MQ=255)
                         aTCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCa                                                                                                                       <  2:1597874/148‑1 (MQ=255)
                          tCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCaa                                                                                                                      >  2:3380894/1‑148 (MQ=255)
                             tCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACtt                                                                                                                   >  2:323715/1‑148 (MQ=255)
                              cACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTc                                                                                                                  <  1:3058806/148‑1 (MQ=255)
                                   tGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCgggg                                                                                                             <  2:2837537/148‑1 (MQ=255)
                                    gTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGt                                                                                                            >  2:1873604/1‑148 (MQ=255)
                                     tACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTa                                                                                                           <  2:2955070/148‑1 (MQ=255)
                                      aCGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAg                                                                                                          <  1:3411798/148‑1 (MQ=255)
                                      aCGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAg                                                                                                          >  2:1207145/1‑148 (MQ=255)
                                       cGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGc                                                                                                         >  2:229836/1‑148 (MQ=255)
                                        ggAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCg                                                                                                        <  2:3071359/148‑1 (MQ=255)
                                          aGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGAc                                                                                                      >  1:984024/1‑148 (MQ=255)
                                          aGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGAc                                                                                                      >  1:1399984/1‑148 (MQ=255)
                                          aGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGAc                                                                                                      >  2:1674482/1‑148 (MQ=255)
                                               ttCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATcgc                                                                                                 >  2:3144211/1‑148 (MQ=255)
                                                  aGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGAt                                                                                              <  1:1252616/148‑1 (MQ=255)
                                                   gTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATc                                                                                             >  2:2442406/1‑148 (MQ=255)
                                                    tGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATcc                                                                                            <  1:1611333/148‑1 (MQ=255)
                                                    tGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGACCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATcc                                                                                            >  1:2512751/1‑148 (MQ=255)
                                                     gCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGNCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATccc                                                                                           <  1:1810835/148‑1 (MQ=255)
                                                     gCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATccc                                                                                           <  1:934770/148‑1 (MQ=255)
                                                        tCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGt                                                                                        >  1:2670000/1‑148 (MQ=255)
                                                        tCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGt                                                                                        >  1:459211/1‑148 (MQ=255)
                                                          ggAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTg                                                                                      <  2:2151736/148‑1 (MQ=255)
                                                             gTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATg                                                                                   <  2:2795162/148‑1 (MQ=255)
                                                              tACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGc                                                                                  >  1:2971685/1‑148 (MQ=255)
                                                              tACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGc                                                                                  <  2:2760005/148‑1 (MQ=255)
                                                                cTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACCTCGCGATCCCAGTTGATGCCg                                                                                >  1:1104439/1‑148 (MQ=255)
                                                                 ttGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGa                                                                               <  2:3363066/148‑1 (MQ=255)
                                                                  tGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGaa                                                                              >  1:1512245/1‑148 (MQ=255)
                                                                  tGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGaa                                                                              <  1:2104432/148‑1 (MQ=255)
                                                                     tccccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagta                                                                           >  2:346586/3‑148 (MQ=255)
                                                                     tACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagta                                                                           <  2:2362743/148‑1 (MQ=255)
                                                                       cccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagtagt                                                                         <  1:878845/148‑1 (MQ=255)
                                                                       cccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAagtagt                                                                         <  2:438289/148‑1 (MQ=255)
                                                                        ccAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtg                                                                        >  1:1759955/1‑148 (MQ=255)
                                                                         cAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAgtgt                                                                       <  1:2726024/148‑1 (MQ=255)
                                                                          aGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGtt                                                                      <  2:1568880/148‑1 (MQ=255)
                                                                           gCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGttt                                                                     <  2:1374030/148‑1 (MQ=255)
                                                                              ggAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTAcc                                                                  >  1:1000387/1‑148 (MQ=255)
                                                                              ggAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTAcc                                                                  <  1:2742974/148‑1 (MQ=255)
                                                                                  gATGTGACCAACTGCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCg                                                              <  2:1310130/148‑1 (MQ=255)
                                                                                   aTGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGa                                                             >  1:1688202/1‑148 (MQ=255)
                                                                                   aTGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGa                                                             <  2:646174/148‑1 (MQ=255)
                                                                                    tgtgACCAACTTCGATACCACGTGTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCAAt                                                            <  2:2467589/148‑1 (MQ=255)
                                                                                      tgACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGGTATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGt                                                          <  2:2459621/148‑1 (MQ=255)
                                                                                      tgACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGt                                                          <  2:2204081/148‑1 (MQ=255)
                                                                                      tgACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGt                                                          <  1:774792/148‑1 (MQ=255)
                                                                                      tgACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGt                                                          <  2:544481/148‑1 (MQ=255)
                                                                                       gACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGtt                                                         <  1:201761/148‑1 (MQ=255)
                                                                                         ccAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAg                                                       <  2:2157995/148‑1 (MQ=255)
                                                                                            aCTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCAc                                                    <  2:2017210/148‑1 (MQ=255)
                                                                                             cTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCAcc                                                   >  2:2201899/1‑148 (MQ=255)
                                                                                                    aCCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCg                                            <  1:3560245/148‑1 (MQ=255)
                                                                                                     ccACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGa                                           >  2:1652660/1‑148 (MQ=255)
                                                                                                       aCGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGaaa                                         <  1:1341011/148‑1 (MQ=255)
                                                                                                        cGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAAt                                        >  2:2891101/1‑148 (MQ=255)
                                                                                                        cGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAAt                                        >  2:2890644/1‑148 (MQ=255)
                                                                                                          tttGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCa                                      <  1:1875252/148‑1 (MQ=255)
                                                                                                           ttGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGGAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCAc                                     >  2:1956291/1‑148 (MQ=255)
                                                                                                             gATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTc                                   <  2:233331/148‑1 (MQ=255)
                                                                                                             gATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTc                                   >  2:2644158/1‑148 (MQ=255)
                                                                                                             gATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTc                                   <  1:2229433/148‑1 (MQ=255)
                                                                                                              aTCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCa                                  >  1:2946973/1‑148 (MQ=255)
                                                                                                               tCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCAt                                 >  1:3230018/1‑148 (MQ=255)
                                                                                                               tCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCAt                                 <  1:3380894/148‑1 (MQ=255)
                                                                                                                 agcagcCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcg                               <  1:3124829/148‑1 (MQ=255)
                                                                                                                  gcagcCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcgc                              >  1:2654516/1‑148 (MQ=255)
                                                                                                                    agcCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcgccg                            <  2:62438/148‑1 (MQ=255)
                                                                                                                    agcCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATcgccg                            <  1:2860861/148‑1 (MQ=255)
                                                                                                                      ccTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCa                          <  2:2823972/148‑1 (MQ=255)
                                                                                                                      ccTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCa                          >  2:3212072/1‑148 (MQ=255)
                                                                                                                      ccTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCa                          >  2:1911772/1‑148 (MQ=255)
                                                                                                                         aCCCTGACCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacg                       <  2:2512751/148‑1 (MQ=255)
                                                                                                                          cccTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacgg                      >  1:795173/1‑148 (MQ=255)
                                                                                                                             tGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtac                   >  2:1797721/1‑148 (MQ=255)
                                                                                                                             tGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtac                   >  1:644869/1‑148 (MQ=255)
                                                                                                                               gCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAacggtacgg                 >  2:2904483/1‑148 (MQ=255)
                                                                                                                                   tCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCaa             >  2:1091055/1‑148 (MQ=255)
                                                                                                                                   tCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCaa             <  1:3004309/148‑1 (MQ=255)
                                                                                                                                    ccGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAt            >  2:532658/1‑148 (MQ=255)
                                                                                                                                    ccGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAAt            >  2:1537081/1‑148 (MQ=255)
                                                                                                                                       ggCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcac         <  1:155311/148‑1 (MQ=255)
                                                                                                                                       ggCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcac         >  2:372845/1‑148 (MQ=255)
                                                                                                                                       ggCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcac         >  1:2383982/1‑148 (MQ=255)
                                                                                                                                        gCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATcacc        <  2:2543106/148‑1 (MQ=255)
                                                                                                                                           tGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCaccacc     <  1:3543818/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGA  >  CP009273/213939‑214228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGG  >  CP009273/213940‑214233
                                                                                                                                                |                                                                                                                                                     
CATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCAGCCGGGCTTGGATCTCCAGC                                                                                                                                                  <  SRR19973066.2820338/150‑1 (MQ=60)
  TCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCA                                                                                                                                                <  SRR19973066.3227999/150‑1 (MQ=60)
      CAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCAC                                                                                                                                            >  SRR19973066.1679231/1‑150 (MQ=60)
      CAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCAC                                                                                                                                            <  SRR19973066.3354250/150‑1 (MQ=60)
      CAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCAC                                                                                                                                            <  SRR19973066.3354351/150‑1 (MQ=60)
          ATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTA                                                                                                                                        >  SRR19973066.235176/1‑150 (MQ=60)
          ATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTA                                                                                                                                        >  SRR19973066.235497/1‑150 (MQ=60)
          ATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTA                                                                                                                                        <  SRR19973066.290329/150‑1 (MQ=60)
            TTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACG                                                                                                                                      >  SRR19973066.2210181/1‑150 (MQ=60)
            TTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACG                                                                                                                                      <  SRR19973066.2594293/150‑1 (MQ=60)
            TTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACG                                                                                                                                      <  SRR19973066.2594319/150‑1 (MQ=60)
                   CGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCT                                                                                                                               <  SRR19973066.1419484/150‑1 (MQ=60)
                    GGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTG                                                                                                                              >  SRR19973066.850609/1‑150 (MQ=60)
                    GGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTG                                                                                                                              <  SRR19973066.813716/150‑1 (MQ=60)
                      CCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCA                                                                                                                            >  SRR19973066.229459/1‑150 (MQ=60)
                         TCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACT                                                                                                                         <  SRR19973066.1691988/150‑1 (MQ=60)
                          CTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTT                                                                                                                        <  SRR19973066.2674849/150‑1 (MQ=60)
                             CACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCG                                                                                                                     <  SRR19973066.3079266/150‑1 (MQ=60)
                             CACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCG                                                                                                                     >  SRR19973066.704831/1‑150 (MQ=60)
                              ACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGG                                                                                                                    <  SRR19973066.1802512/150‑1 (MQ=60)
                                 CTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGT                                                                                                                 >  SRR19973066.3283725/1‑150 (MQ=60)
                                     ACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCG                                                                                                             <  SRR19973066.3434606/150‑1 (MQ=60)
                                       GGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGAC                                                                                                           >  SRR19973066.1410112/1‑150 (MQ=60)
                                       GGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGAC                                                                                                           >  SRR19973066.991285/1‑150 (MQ=60)
                                       GGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGAC                                                                                                           >  SRR19973066.1410070/1‑150 (MQ=60)
                                         AGGCTCTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACAT                                                                                                         <  SRR19973066.596399/150‑1 (MQ=60)
                                                CAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATC                                                                                                  >  SRR19973066.56195/1‑150 (MQ=60)
                                                 AGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCC                                                                                                 <  SRR19973066.1261623/150‑1 (MQ=60)
                                                 AGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCC                                                                                                 <  SRR19973066.266044/150‑1 (MQ=60)
                                                 AGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCC                                                                                                 <  SRR19973066.1261967/150‑1 (MQ=60)
                                                 AGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGACCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCC                                                                                                 >  SRR19973066.2529776/1‑150 (MQ=60)
                                                 AGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGACCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCC                                                                                                 >  SRR19973066.2529852/1‑150 (MQ=60)
                                                 AGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGACCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCC                                                                                                 >  SRR19973066.1043209/1‑150 (MQ=60)
                                                  GTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCC                                                                                                <  SRR19973066.2701542/150‑1 (MQ=60)
                                                   TGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCA                                                                                               <  SRR19973066.1623021/150‑1 (MQ=60)
                                                    GCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGNCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAG                                                                                              <  SRR19973066.1823710/150‑1 (MQ=60)
                                                    GCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAG                                                                                              <  SRR19973066.941562/150‑1 (MQ=60)
                                                    GCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAG                                                                                              <  SRR19973066.936871/150‑1 (MQ=60)
                                                     CTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGT                                                                                             >  SRR19973066.2687849/1‑150 (MQ=60)
                                                     CTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGT                                                                                             >  SRR19973066.462729/1‑150 (MQ=60)
                                                           AGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGC                                                                                       >  SRR19973066.2991327/1‑150 (MQ=60)
                                                            GTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCC                                                                                      <  SRR19973066.2579607/150‑1 (MQ=60)
                                                             TACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACCTCGCGATCCCAGTTGATGCCG                                                                                     >  SRR19973066.1112444/1‑150 (MQ=60)
                                                               CTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAA                                                                                   >  SRR19973066.1522844/1‑150 (MQ=60)
                                                                 TGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGT                                                                                 <  SRR19973066.2118930/150‑1 (MQ=60)
                                                                 TGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGT                                                                                 <  SRR19973066.1900291/150‑1 (MQ=60)
                                                                    TACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGT                                                                              <  SRR19973066.3524865/150‑1 (MQ=60)
                                                                     ACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTG                                                                             >  SRR19973066.1772594/1‑150 (MQ=60)
                                                                      CCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGT                                                                            <  SRR19973066.884103/150‑1 (MQ=60)
                                                                      CCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGT                                                                            <  SRR19973066.885106/150‑1 (MQ=60)
                                                                       CCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTT                                                                           <  SRR19973066.3459582/150‑1 (MQ=60)
                                                                        CAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTT                                                                          <  SRR19973066.2744357/150‑1 (MQ=60)
                                                                           CTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACC                                                                       >  SRR19973066.1523929/1‑150 (MQ=60)
                                                                           CTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACC                                                                       <  SRR19973066.2835866/150‑1 (MQ=60)
                                                                           CTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACC                                                                       >  SRR19973066.1523928/1‑150 (MQ=60)
                                                                           CTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACC                                                                       >  SRR19973066.1007760/1‑150 (MQ=60)
                                                                            TGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCA                                                                      >  SRR19973066.1509017/1‑150 (MQ=60)
                                                                             GGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCAT                                                                     <  SRR19973066.2761430/150‑1 (MQ=60)
                                                                             GGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCAT                                                                     <  SRR19973066.2761088/150‑1 (MQ=60)
                                                                               AAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTTGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCG                                                                   >  SRR19973066.979015/1‑150 (MQ=60)
                                                                                AGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGA                                                                  >  SRR19973066.1700489/1‑150 (MQ=60)
                                                                                    GTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTT                                                              <  SRR19973066.1664608/150‑1 (MQ=60)
                                                                                     TGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCCGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTA                                                             <  SRR19973066.3417185/150‑1 (MQ=60)
                                                                                     TGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTA                                                             <  SRR19973066.780521/150‑1 (MQ=60)
                                                                                      GACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAG                                                            <  SRR19973066.203469/150‑1 (MQ=60)
                                                                                            CTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCCGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAG                                                      <  SRR19973066.1590102/150‑1 (MQ=60)
                                                                                            CTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAG                                                      <  SRR19973066.3295789/150‑1 (MQ=60)
                                                                                                 ATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCG                                                 >  SRR19973066.2394299/1‑150 (MQ=60)
                                                                                                  TACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGA                                                >  SRR19973066.2067415/1‑150 (MQ=60)
                                                                                                   ACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAA                                               <  SRR19973066.3583855/150‑1 (MQ=60)
                                                                                                   ACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAA                                               <  SRR19973066.3583838/150‑1 (MQ=60)
                                                                                                      ACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATC                                            <  SRR19973066.3138007/150‑1 (MQ=60)
                                                                                                      ACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATC                                            <  SRR19973066.1350746/150‑1 (MQ=60)
                                                                                                      ACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATC                                            <  SRR19973066.1350884/150‑1 (MQ=60)
                                                                                                        GTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCAC                                          <  SRR19973066.1215567/150‑1 (MQ=60)
                                                                                                         TTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCCGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACT                                         <  SRR19973066.1888640/150‑1 (MQ=60)
                                                                                                           TGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCA                                       >  SRR19973066.2966536/1‑150 (MQ=60)
                                                                                                           TGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCA                                       >  SRR19973066.1709884/1‑150 (MQ=60)
                                                                                                           TGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCA                                       >  SRR19973066.1710054/1‑150 (MQ=60)
                                                                                                            GATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCAT                                      >  SRR19973066.3251357/1‑150 (MQ=60)
                                                                                                            GATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCAT                                      <  SRR19973066.2244832/150‑1 (MQ=60)
                                                                                                              TCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCG                                    <  SRR19973066.3403445/150‑1 (MQ=60)
                                                                                                               CAGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGC                                   >  SRR19973066.2672323/1‑150 (MQ=60)
                                                                                                                AGCAGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCC                                  <  SRR19973066.3145697/150‑1 (MQ=60)
                                                                                                                   AGCCTACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCA                               <  SRR19973066.2880073/150‑1 (MQ=60)
                                                                                                                       TACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGG                           >  SRR19973066.801012/1‑150 (MQ=60)
                                                                                                                       TACCCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGG                           >  SRR19973066.800992/1‑150 (MQ=60)
                                                                                                                          CCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTAC                        <  SRR19973066.2968876/150‑1 (MQ=60)
                                                                                                                          CCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTAC                        <  SRR19973066.3035963/150‑1 (MQ=60)
                                                                                                                          CCTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTAC                        >  SRR19973066.649541/1‑150 (MQ=60)
                                                                                                                           CTGGCCATCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACG                       <  SRR19973066.868286/150‑1 (MQ=60)
                                                                                                                                  TCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATC                <  SRR19973066.1951789/150‑1 (MQ=60)
                                                                                                                                  TCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATC                <  SRR19973066.1403943/150‑1 (MQ=60)
                                                                                                                                  TCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATC                <  SRR19973066.3024254/150‑1 (MQ=60)
                                                                                                                                  TCCGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATC                <  SRR19973066.3024290/150‑1 (MQ=60)
                                                                                                                                    CGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCAC              >  SRR19973066.37090/1‑150 (MQ=60)
                                                                                                                                    CGGGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCAC              >  SRR19973066.2400056/1‑150 (MQ=60)
                                                                                                                                      GGGTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCA            <  SRR19973066.3001951/150‑1 (MQ=60)
                                                                                                                                      GGCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCA            <  SRR19973066.156751/150‑1 (MQ=60)
                                                                                                                                       GCTTGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCAC           >  SRR19973066.1874411/1‑150 (MQ=60)
                                                                                                                                          TGGATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGG        <  SRR19973066.3567351/150‑1 (MQ=60)
                                                                                                                                            GATCTCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAA      >  SRR19973066.1418502/1‑150 (MQ=60)
                                                                                                                                                TCCAGCCTCCACGTTACGGATATCTTCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATAACTCATCGCCGCATCGGTACGGTCAATCACCACCGGAATCGG  <  SRR19973066.89403/150‑1 (MQ=60)
                                                                                                                                                |                                                                                                                                                     
CATCGTCAGGATTTGGTTACGGCCATCTTCACCCTGTACGGAGGCTTTCAGTGCTTCGGAGTACTTGGTACCCAGCTGGAAGATGTGACCAACTTCGATACCACGTTTGATCAGCAGCCTACCCTGGCCATCCGGGCTTGGATCGCCAGCCACCACGTTACGGATATCTGCAACTTCCGGGGTAGCGACATCGCGATCCCAGTTGATGCCGAAGTAGTGTTTACCATCGATGTTAGCACCAGCAGCGAAATCACTCATCGCCGCAACGGTACGGTCAATCACCACCGGAATCGG  >  CP009273/213940‑214233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑