Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 344,800 G→T 100% E136D (GAG→GAT)  prpB → 2‑methylisocitrate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273344,8000GT99.3% 478.1 / ‑4.1 150E136D (GAG→GAT) prpB2‑methylisocitrate lyase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (82/67);  total (82/68)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 152 total aligned reads displayed.

TCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGAT  >  CP009273/344655‑344943
                                                                                                                                                 |                                                                                                                                               
tCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGatat                                                                                                                                               <  1:2277621/148‑1 (MQ=255)
tCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGatat                                                                                                                                               >  2:932928/1‑148 (MQ=255)
 cGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtg                                                                                                                                              >  2:272781/1‑148 (MQ=255)
   gTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtggt                                                                                                                                            <  2:2935773/148‑1 (MQ=255)
    ttttGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtggtg                                                                                                                                           >  2:1558191/1‑148 (MQ=255)
    ttttGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtggtg                                                                                                                                           >  1:618973/1‑148 (MQ=255)
    ttttGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtggtg                                                                                                                                           >  2:3008385/1‑148 (MQ=255)
    ttttGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtggtg                                                                                                                                           >  2:3008381/1‑148 (MQ=255)
          ttcttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTggatcgg                                                                                                                                     <  2:1996562/148‑1 (MQ=255)
            cttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTggatcggat                                                                                                                                   <  1:677583/148‑1 (MQ=255)
            cttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTggatcggat                                                                                                                                   <  1:1725569/148‑1 (MQ=255)
            cttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTggatcggat                                                                                                                                   <  1:1725567/148‑1 (MQ=255)
             ttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTggatcggatc                                                                                                                                  <  2:2190213/148‑1 (MQ=255)
              tcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATcc                                                                                                                                 >  1:312333/1‑148 (MQ=255)
              tcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATcc                                                                                                                                 >  1:2900012/1‑148 (MQ=255)
              tcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATcc                                                                                                                                 >  1:2952197/1‑148 (MQ=255)
                    tttAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCgcgg                                                                                                                           >  1:147378/1‑148 (MQ=255)
                    tttAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCgcgg                                                                                                                           >  2:117669/1‑148 (MQ=255)
                     ttAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCgcggc                                                                                                                          >  2:2832482/1‑148 (MQ=255)
                           tGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGa                                                                                                                    >  1:266999/1‑148 (MQ=255)
                            ggCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGAt                                                                                                                   <  2:618973/148‑1 (MQ=255)
                            ggCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTAGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGAt                                                                                                                   <  1:3268080/148‑1 (MQ=255)
                                 gcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGaa                                                                                                              <  1:1023480/148‑1 (MQ=255)
                                  cACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGaaa                                                                                                             >  2:76009/1‑148 (MQ=255)
                                  cACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGaaa                                                                                                             <  2:254858/148‑1 (MQ=255)
                                   aCCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGaaaa                                                                                                            <  1:1372009/148‑1 (MQ=255)
                                    ccGTGACATCGATGATTACAGCCGGTGCGGCAGGATTGCAGATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATTAAGCGATCGTCTCGAAAGATGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAAc                                                                                                           <  1:1268061/148‑1 (MQ=255)
                                    ccGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAAc                                                                                                           <  1:828363/148‑1 (MQ=255)
                                    ccGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAAc                                                                                                           <  1:120150/148‑1 (MQ=255)
                                       tGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGa                                                                                                        <  1:1949533/148‑1 (MQ=255)
                                            tCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTg                                                                                                   <  1:1043029/148‑1 (MQ=255)
                                               atgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtt                                                                                                >  1:2534025/1‑148 (MQ=255)
                                               atgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtt                                                                                                <  1:239752/148‑1 (MQ=255)
                                               atgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtt                                                                                                >  1:2354959/1‑148 (MQ=255)
                                               atgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtt                                                                                                >  1:1815267/1‑148 (MQ=255)
                                                tgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAttt                                                                                               <  1:3311528/148‑1 (MQ=255)
                                                tgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAttt                                                                                               >  2:1410080/1‑148 (MQ=255)
                                                 gatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtttt                                                                                              <  1:1642202/148‑1 (MQ=255)
                                                 gatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtttt                                                                                              <  1:3279848/148‑1 (MQ=255)
                                                  atTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtg                                                                                             <  1:1238839/148‑1 (MQ=255)
                                                  atTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtg                                                                                             <  1:1238802/148‑1 (MQ=255)
                                                   ttAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtgt                                                                                            >  1:2739665/1‑148 (MQ=255)
                                                   ttAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtgt                                                                                            >  2:3261848/1‑148 (MQ=255)
                                                    tAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGTTGGAATATATGGTGTATCGTATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtgtg                                                                                           <  1:3249481/148‑1 (MQ=255)
                                                    tAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtgtg                                                                                           <  2:1998457/148‑1 (MQ=255)
                                                    tAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtgtg                                                                                           >  2:2783741/1‑148 (MQ=255)
                                                    tAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtgtg                                                                                           >  2:2783826/1‑148 (MQ=255)
                                                    tAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTtgtg                                                                                           >  1:3124759/1‑148 (MQ=255)
                                                       aGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATc                                                                                        >  1:3273604/1‑148 (MQ=255)
                                                          cGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATg                                                                                     <  2:3171291/148‑1 (MQ=255)
                                                                    ggATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCg                                                                           <  2:626825/148‑1 (MQ=255)
                                                                    ggATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCg                                                                           >  1:658002/1‑148 (MQ=255)
                                                                     gATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGa                                                                          >  2:855089/1‑148 (MQ=255)
                                                                     gATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGa                                                                          >  1:1204980/1‑148 (MQ=255)
                                                                       ttGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATg                                                                        >  2:3283747/1‑148 (MQ=255)
                                                                        tGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGc                                                                       >  1:161671/1‑148 (MQ=255)
                                                                            tatTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTg                                                                   >  1:2943591/1‑148 (MQ=255)
                                                                            tatTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTg                                                                   >  1:1296494/1‑148 (MQ=255)
                                                                             atTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTgg                                                                  >  1:1819975/1‑148 (MQ=255)
                                                                               tGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCg                                                                >  2:1928058/1‑148 (MQ=255)
                                                                               tGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCg                                                                >  1:2856160/1‑148 (MQ=255)
                                                                                 aaGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGt                                                              >  1:2912623/1‑148 (MQ=255)
                                                                                   gATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTag                                                            <  1:1793545/148‑1 (MQ=255)
                                                                                   gATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTag                                                            >  1:2078956/1‑148 (MQ=255)
                                                                                    aTCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTaga                                                           <  1:258598/148‑1 (MQ=255)
                                                                                           tGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTg                                                    <  2:234897/148‑1 (MQ=255)
                                                                                                  aaaCGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATgcgg                                             >  2:1778873/1‑148 (MQ=255)
                                                                                                  aaaCGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATgcgg                                             >  2:1778976/1‑148 (MQ=255)
                                                                                                    aCGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATgcggcg                                           <  1:2185674/148‑1 (MQ=255)
                                                                                                     cGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGa                                          <  1:393944/148‑1 (MQ=255)
                                                                                                        tGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCg                                       >  2:1180842/1‑148 (MQ=255)
                                                                                                         gCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGa                                      <  2:2971161/148‑1 (MQ=255)
                                                                                                         gCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGa                                      <  2:2969266/148‑1 (MQ=255)
                                                                                                          cGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAg                                     <  1:1981202/148‑1 (MQ=255)
                                                                                                              cATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTg                                 <  2:330637/148‑1 (MQ=255)
                                                                                                              cATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTg                                 >  2:1362724/1‑148 (MQ=255)
                                                                                                               aTCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTgc                                >  2:1011762/1‑148 (MQ=255)
                                                                                                                tCGTCAGAATAAAGAGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCTCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTTATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTgcg                               >  2:1670304/1‑148 (MQ=255)
                                                                                                                 cGTCCGAATAAAGCGATCGTCTCGAATGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTTATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGTGGGGCTGGATGCGGCGATCGAGCGTgcgc                              >  2:898783/1‑148 (MQ=255)
                                                                                                                 cGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTgcgc                              >  2:1274831/1‑148 (MQ=255)
                                                                                                                    ccGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAgg                           >  1:2789233/1‑148 (MQ=255)
                                                                                                                       aaTAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCt                        >  1:2666094/1‑148 (MQ=255)
                                                                                                                        aTAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTa                       >  1:1798265/1‑148 (MQ=255)
                                                                                                                        aTAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTa                       >  1:961228/1‑148 (MQ=255)
                                                                                                                          aaaGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATg                     <  1:1455218/148‑1 (MQ=255)
                                                                                                                          aaaGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATg                     <  1:1455000/148‑1 (MQ=255)
                                                                                                                           aaGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGt                    <  2:2720937/148‑1 (MQ=255)
                                                                                                                              cGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGa                 >  1:1423153/1‑148 (MQ=255)
                                                                                                                                 tCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGc              >  2:1485634/1‑148 (MQ=255)
                                                                                                                                  cGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCg             <  1:272781/148‑1 (MQ=255)
                                                                                                                                  cGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCg             >  1:1341475/1‑148 (MQ=255)
                                                                                                                                  cGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCg             <  1:1664076/148‑1 (MQ=255)
                                                                                                                                    tctcGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCggg           >  2:2176797/1‑148 (MQ=255)
                                                                                                                                    tctcGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCggg           <  1:3008385/148‑1 (MQ=255)
                                                                                                                                     ctcGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGt          >  2:968511/1‑148 (MQ=255)
                                                                                                                                       cGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGc        >  1:2329445/1‑148 (MQ=255)
                                                                                                                                          aagaagATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCga     >  1:2611383/1‑148 (MQ=255)
                                                                                                                                           agaagaTATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCgag    <  1:1705643/148‑1 (MQ=255)
                                                                                                                                           agaagaTATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCgag    <  2:1295014/148‑1 (MQ=255)
                                                                                                                                             aagaTATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGAt  >  2:2643011/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGAT  >  CP009273/344655‑344943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTT  >  CP009273/344652‑344949
                                                                                                                                                    |                                                                                                                                                     
ATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATA                                                                                                                                                      >  SRR19973070.2693086/1‑150 (MQ=60)
 TATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAT                                                                                                                                                     <  SRR19973070.2857211/150‑1 (MQ=60)
  ATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATG                                                                                                                                                    >  SRR19973070.2738499/1‑150 (MQ=60)
   TCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGG                                                                                                                                                   <  SRR19973070.2292751/150‑1 (MQ=60)
    CGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGT                                                                                                                                                  <  SRR19973070.3272034/150‑1 (MQ=60)
     GGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTG                                                                                                                                                 >  SRR19973070.623324/1‑150 (MQ=60)
           GGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGG                                                                                                                                           >  SRR19973070.333048/1‑150 (MQ=60)
               CTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCC                                                                                                                                       >  SRR19973070.2971198/1‑150 (MQ=60)
               CTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCC                                                                                                                                       >  SRR19973070.314573/1‑150 (MQ=60)
               CTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCC                                                                                                                                       >  SRR19973070.2918774/1‑150 (MQ=60)
               CTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCC                                                                                                                                       >  SRR19973070.1259543/1‑150 (MQ=60)
               CTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCC                                                                                                                                       <  SRR19973070.682415/150‑1 (MQ=60)
               CTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCC                                                                                                                                       <  SRR19973070.1737515/150‑1 (MQ=60)
               CTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCC                                                                                                                                       <  SRR19973070.1737513/150‑1 (MQ=60)
                TTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCG                                                                                                                                      >  SRR19973070.2197366/1‑150 (MQ=60)
                     CCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGG                                                                                                                                 >  SRR19973070.148631/1‑150 (MQ=60)
                            CGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGA                                                                                                                          >  SRR19973070.268765/1‑150 (MQ=60)
                               GGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTAGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGC                                                                                                                       <  SRR19973070.3289103/150‑1 (MQ=60)
                               GGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTAGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGC                                                                                                                       <  SRR19973070.3289104/150‑1 (MQ=60)
                                    GCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAA                                                                                                                  <  SRR19973070.1030738/150‑1 (MQ=60)
                                     CACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAAC                                                                                                                 <  SRR19973070.602409/150‑1 (MQ=60)
                                      ACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACC                                                                                                                <  SRR19973070.1381337/150‑1 (MQ=60)
                                       CCGTGACATCGATGATTACAGCCGGTGCGGCAGGATTGCAGATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATTAAGCGATCGTCTCGAAAGATGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCG                                                                                                               <  SRR19973070.1276934/150‑1 (MQ=60)
                                       CCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCG                                                                                                               <  SRR19973070.833991/150‑1 (MQ=60)
                                       CCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCG                                                                                                               <  SRR19973070.834007/150‑1 (MQ=60)
                                       CCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCG                                                                                                               <  SRR19973070.121227/150‑1 (MQ=60)
                                       CCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCG                                                                                                               <  SRR19973070.117525/150‑1 (MQ=60)
                                          TGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATC                                                                                                            <  SRR19973070.1962627/150‑1 (MQ=60)
                                               TCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAT                                                                                                       <  SRR19973070.1050386/150‑1 (MQ=60)
                                                CGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATT                                                                                                      >  SRR19973070.2370555/1‑150 (MQ=60)
                                                CGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATT                                                                                                      >  SRR19973070.2550585/1‑150 (MQ=60)
                                                CGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATT                                                                                                      >  SRR19973070.2551005/1‑150 (MQ=60)
                                                CGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATT                                                                                                      >  SRR19973070.1827779/1‑150 (MQ=60)
                                                  ATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTT                                                                                                    <  SRR19973070.241413/150‑1 (MQ=60)
                                                  ATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTT                                                                                                    <  SRR19973070.240736/150‑1 (MQ=60)
                                                   TGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTG                                                                                                   <  SRR19973070.3332708/150‑1 (MQ=60)
                                                   TGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTG                                                                                                   <  SRR19973070.2304050/150‑1 (MQ=60)
                                                    GATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGT                                                                                                  <  SRR19973070.3300920/150‑1 (MQ=60)
                                                    GATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGT                                                                                                  >  SRR19973070.2757294/1‑150 (MQ=60)
                                                    GATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGT                                                                                                  <  SRR19973070.1653558/150‑1 (MQ=60)
                                                     ATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTG                                                                                                 >  SRR19973070.3144934/1‑150 (MQ=60)
                                                     ATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTG                                                                                                 >  SRR19973070.3144910/1‑150 (MQ=60)
                                                     ATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTG                                                                                                 <  SRR19973070.1247517/150‑1 (MQ=60)
                                                     ATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTG                                                                                                 <  SRR19973070.1247480/150‑1 (MQ=60)
                                                     ATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTG                                                                                                 <  SRR19973070.1247477/150‑1 (MQ=60)
                                                       TAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGTTGGAATATATGGTGTATCGTATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGAT                                                                                               <  SRR19973070.3270421/150‑1 (MQ=60)
                                                       TAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGAT                                                                                               <  SRR19973070.1623706/150‑1 (MQ=60)
                                                        AAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATC                                                                                              >  SRR19973070.3294649/1‑150 (MQ=60)
                                                              GGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCG                                                                                        <  SRR19973070.1740537/150‑1 (MQ=60)
                                                                     CAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCG                                                                                 >  SRR19973070.662763/1‑150 (MQ=60)
                                                                     CAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCG                                                                                 >  SRR19973070.662689/1‑150 (MQ=60)
                                                                      AGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGA                                                                                >  SRR19973070.1213439/1‑150 (MQ=60)
                                                                         ATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGC                                                                             >  SRR19973070.163000/1‑150 (MQ=60)
                                                                         ATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGC                                                                             >  SRR19973070.2943360/1‑150 (MQ=60)
                                                                             CATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTG                                                                         >  SRR19973070.1305517/1‑150 (MQ=60)
                                                                             CATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTG                                                                         >  SRR19973070.2962555/1‑150 (MQ=60)
                                                                              ATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGG                                                                        >  SRR19973070.1832513/1‑150 (MQ=60)
                                                                                ATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCG                                                                      >  SRR19973070.2874660/1‑150 (MQ=60)
                                                                                  TGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGT                                                                    >  SRR19973070.2931448/1‑150 (MQ=60)
                                                                                    AAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAG                                                                  >  SRR19973070.2092947/1‑150 (MQ=60)
                                                                                     AGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGA                                                                 >  SRR19973070.291037/1‑150 (MQ=60)
                                                                                      GATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAG                                                                <  SRR19973070.1805935/150‑1 (MQ=60)
                                                                                       ATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGG                                                               <  SRR19973070.260333/150‑1 (MQ=60)
                                                                                                     AAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGCATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCATGTTTTTGTGAGAAGGGCGCGCACCGAGGCTCTGGCGGTAGAGGGGCTGGATGCGGCG                                                 >  SRR19973070.1335366/1‑150 (MQ=60)
                                                                                                       ACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGAT                                               <  SRR19973070.2200124/150‑1 (MQ=60)
                                                                                                        CGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATC                                              <  SRR19973070.396836/150‑1 (MQ=60)
                                                                                                        CGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATC                                              >  SRR19973070.1813288/1‑150 (MQ=60)
                                                                                                             CGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCG                                         <  SRR19973070.1994607/150‑1 (MQ=60)
                                                                                                             CGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCG                                         <  SRR19973070.1419689/150‑1 (MQ=60)
                                                                                                             CGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCG                                         <  SRR19973070.1994594/150‑1 (MQ=60)
                                                                                                                     GTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGG                                 >  SRR19973070.2807200/1‑150 (MQ=60)
                                                                                                                        CGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCT                              >  SRR19973070.2683414/1‑150 (MQ=60)
                                                                                                                        CGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCT                              >  SRR19973070.2683263/1‑150 (MQ=60)
                                                                                                                         GAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTA                             >  SRR19973070.1810683/1‑150 (MQ=60)
                                                                                                                         GAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTA                             >  SRR19973070.968084/1‑150 (MQ=60)
                                                                                                                             AAAGCGATCGTCTCGACAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGAGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTT                         <  SRR19973070.1465184/150‑1 (MQ=60)
                                                                                                                             AAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTT                         <  SRR19973070.1465360/150‑1 (MQ=60)
                                                                                                                             AAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTT                         <  SRR19973070.1465139/150‑1 (MQ=60)
                                                                                                                               AGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGA                       >  SRR19973070.1432936/1‑150 (MQ=60)
                                                                                                                                 CGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAG                     >  SRR19973070.2770240/1‑150 (MQ=60)
                                                                                                                                   ATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCG                   >  SRR19973070.1350690/1‑150 (MQ=60)
                                                                                                                                     CGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGG                 <  SRR19973070.1675408/150‑1 (MQ=60)
                                                                                                                                     CGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGG                 <  SRR19973070.1675517/150‑1 (MQ=60)
                                                                                                                                     CGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGG                 <  SRR19973070.274564/150‑1 (MQ=60)
                                                                                                                                       TCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTG               <  SRR19973070.3027592/150‑1 (MQ=60)
                                                                                                                                       TCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTG               <  SRR19973070.3027596/150‑1 (MQ=60)
                                                                                                                                        CTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGC              >  SRR19973070.2344919/1‑150 (MQ=60)
                                                                                                                                           GAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGA           >  SRR19973070.2628423/1‑150 (MQ=60)
                                                                                                                                           GAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGA           >  SRR19973070.1913185/1‑150 (MQ=60)
                                                                                                                                              AGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGAT        <  SRR19973070.1717412/150‑1 (MQ=60)
                                                                                                                                              AGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGAT        <  SRR19973070.1718135/150‑1 (MQ=60)
                                                                                                                                                 AGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTT     >  SRR19973070.3186948/1‑150 (MQ=60)
                                                                                                                                                  GATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTG    >  SRR19973070.172349/1‑150 (MQ=60)
                                                                                                                                                    TATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTT  <  SRR19973070.3008912/150‑1 (MQ=60)
                                                                                                                                                    TATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTT  <  SRR19973070.939584/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGTT  >  CP009273/344652‑344949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑