Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F142 I0 R1	859	274.6	8723680	96.4%	8409627	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	344,800	G→T	91.3%	E136D (GAG→GAT)	prpB →	2‑methylisocitrate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	344,800	0	G	T	91.3%	687.0 / 58.2	242	E136D (GAG→GAT)	prpB	2‑methylisocitrate lyase
Reads supporting (aligned to +/- strand): ref base G (9/12); new base T (100/121); total (109/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01

Only 100 of 244 total aligned reads displayed.

TATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTT  >  CP009273/344653‑344946

tatCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGatat                                                                                                                                                  >  1:3697880/1‑150 (MQ=255)

 atCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtg                                                                                                                                                 <  1:1877640/150‑1 (MQ=255)

 atCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGAtg                                                                                                                                                 <  1:2659667/150‑1 (MQ=255)

  tCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtgg                                                                                                                                                <  1:2364964/150‑1 (MQ=255)

    ggTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtggtg                                                                                                                                              >  1:3941319/1‑150 (MQ=255)

    ggTTTTGGGTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtggtg                                                                                                                                              <  2:1076529/150‑1 (MQ=255)

     gTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtggtgg                                                                                                                                             <  2:1279630/150‑1 (MQ=255)

     gTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtggtgg                                                                                                                                             >  2:3240035/1‑150 (MQ=255)

     gTTTTGGGTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAtggtgg                                                                                                                                             <  2:731690/150‑1 (MQ=255)

      ttttGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTgga                                                                                                                                            >  2:2909814/1‑150 (MQ=255)

       tttGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTggat                                                                                                                                           >  1:1178047/1‑150 (MQ=255)

        ttGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTggatc                                                                                                                                          <  2:1507321/150‑1 (MQ=255)

          ggTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTggatcgg                                                                                                                                        >  1:3437295/1‑150 (MQ=255)

            ttcttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTggatcggat                                                                                                                                      <  1:1645300/150‑1 (MQ=255)

             tcttcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTggatcggatc                                                                                                                                     <  2:3527645/150‑1 (MQ=255)

                tcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCcgc                                                                                                                                  >  2:648155/1‑150 (MQ=255)

                tcGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCcgc                                                                                                                                  <  1:3168372/150‑1 (MQ=255)

                 cGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCcgcg                                                                                                                                 <  1:3152691/150‑1 (MQ=255)

                         aaCGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTg                                                                                                                         >  2:2425441/1‑150 (MQ=255)

                         aaCGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTg                                                                                                                         <  1:2959629/150‑1 (MQ=255)

                          aCGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTgg                                                                                                                        <  2:55720/150‑1 (MQ=255)

                           cGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGa                                                                                                                       <  1:71277/150‑1 (MQ=255)

                           cGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGa                                                                                                                       <  2:2699304/150‑1 (MQ=255)

                             tGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATg                                                                                                                     <  1:1684851/150‑1 (MQ=255)

                             tGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATg                                                                                                                     <  2:4173018/150‑1 (MQ=255)

                              ggCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGc                                                                                                                    <  2:2636417/150‑1 (MQ=255)

                                cgcgcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGa                                                                                                                  <  2:3703720/150‑1 (MQ=255)

                                 gcgcACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGaa                                                                                                                 <  1:278350/150‑1 (MQ=255)

                                    cACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAAc                                                                                                              <  1:2668799/150‑1 (MQ=255)

                                     aCCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAAcc                                                                                                             <  2:917225/150‑1 (MQ=255)

                                        gTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGAt                                                                                                          <  2:4162669/150‑1 (MQ=255)

                                         tGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATc                                                                                                         <  2:1811481/150‑1 (MQ=255)

                                         tGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATc                                                                                                         <  2:1811531/150‑1 (MQ=255)

                                         tGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATc                                                                                                         >  2:4042079/1‑150 (MQ=255)

                                          gAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATcc                                                                                                        >  1:201076/1‑150 (MQ=255)

                                            aaTCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTg                                                                                                      <  1:2353678/150‑1 (MQ=255)

                                            aaTCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTg                                                                                                      <  1:2909814/150‑1 (MQ=255)

                                             aTCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGa                                                                                                     >  1:2961676/1‑150 (MQ=255)

                                             aTCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGa                                                                                                     >  1:332769/1‑150 (MQ=255)

                                                gatgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAttt                                                                                                  <  1:3644053/150‑1 (MQ=255)

                                                gatgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAttt                                                                                                  >  1:2386879/1‑150 (MQ=255)

                                                gatgatTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAttt                                                                                                  <  1:4251622/150‑1 (MQ=255)

                                                 atgatTAAAGCCGGTGCTGAAGGTTTGCATATTGAAGATCTGGTTGGTGCGAATCGCTGCTGTCATCGTCCGAATATAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtttt                                                                                                 <  1:382240/150‑1 (MQ=255)

                                                 atgatTAAAGCCGGTGCGGCAGGAGTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGAtttt                                                                                                 <  1:2267820/150‑1 (MQ=255)

                                                       aaaGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATc                                                                                           <  1:2683084/150‑1 (MQ=255)

                                                        aaGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCa                                                                                          >  1:4101902/1‑150 (MQ=255)

                                                            cGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGgc                                                                                      <  2:3034800/150‑1 (MQ=255)

                                                             ggTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGgcg                                                                                     >  1:1421125/1‑150 (MQ=255)

                                                               tGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGgcgcg                                                                                   <  1:4109177/150‑1 (MQ=255)

                                                                    cAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCg                                                                              <  2:3324994/150‑1 (MQ=255)

                                                                      ggATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAACGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGAt                                                                            <  2:2862718/150‑1 (MQ=255)

                                                                        aTTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCCCCGATGc                                                                          >  2:3101791/1‑150 (MQ=255)

                                                                        aTTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGc                                                                          >  1:856191/1‑150 (MQ=255)

                                                                            cATATTGAAGATCAGGTTGGTGCTAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTg                                                                      >  1:2860470/1‑150 (MQ=255)

                                                                             atatTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTgg                                                                     >  1:3413458/1‑150 (MQ=255)

                                                                              tatTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGc                                                                    <  1:1044114/150‑1 (MQ=255)

                                                                                    aGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTaga                                                              >  1:3512670/1‑150 (MQ=255)

                                                                                      aTCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAgg                                                            <  2:40920/150‑1 (MQ=255)

                                                                                       tCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATACAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAggg                                                           <  2:2326992/150‑1 (MQ=255)

                                                                                          ggTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCt                                                        >  2:4156357/1‑150 (MQ=255)

                                                                                           gTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTg                                                       <  1:641320/150‑1 (MQ=255)

                                                                                            ttGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTgg                                                      <  1:1290421/150‑1 (MQ=255)

                                                                                             tGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGa                                                     >  2:2468036/1‑150 (MQ=255)

                                                                                              ggTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGAt                                                    <  2:136242/150‑1 (MQ=255)

                                                                                                tGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATgc                                                  <  2:2184786/150‑1 (MQ=255)

                                                                                                tGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATgc                                                  >  2:1414016/1‑150 (MQ=255)

                                                                                                   gAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATgcggc                                               <  2:192645/150‑1 (MQ=255)

                                                                                                   gAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATgcggc                                               >  2:2991090/1‑150 (MQ=255)

                                                                                                    aaaCGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATgcggcg                                              <  2:3197510/150‑1 (MQ=255)

                                                                                                    aaaCGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATgcggcg                                              <  2:4051538/150‑1 (MQ=255)

                                                                                                      aCGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGAt                                            <  2:4342949/150‑1 (MQ=255)

                                                                                                      aCGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGAt                                            <  2:82216/150‑1 (MQ=255)

                                                                                                      aCGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGAt                                            <  2:3555688/150‑1 (MQ=255)

                                                                                                        gCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCg                                          >  2:3336159/1‑150 (MQ=255)

                                                                                                         cTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGa                                         >  1:1789701/1‑150 (MQ=255)

                                                                                                             ggTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGt                                     <  1:2286916/150‑1 (MQ=255)

                                                                                                              gTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTg                                    <  1:4146817/150‑1 (MQ=255)

                                                                                                                    gTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAgg                              >  1:1668087/1‑150 (MQ=255)

                                                                                                                     tCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGc                             >  1:1494774/1‑150 (MQ=255)

                                                                                                                      ccGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGcc                            >  1:428524/1‑150 (MQ=255)

                                                                                                                       cGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCt                           >  1:3088516/1‑150 (MQ=255)

                                                                                                                       cGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCt                           <  1:484842/150‑1 (MQ=255)

                                                                                                                       cGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCt                           >  1:531256/1‑150 (MQ=255)

                                                                                                                       cGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCt                           >  1:2597757/1‑150 (MQ=255)

                                                                                                                        gAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTa                          >  2:1680506/1‑150 (MQ=255)

                                                                                                                        gAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTa                          >  2:3868630/1‑150 (MQ=255)

                                                                                                                        gAATAAAGAGATCGTCTCGAAAGAAGATATTGTGGATCGGATCCGCGCGTCGGTGGATGAGAAAAACTATCCAGATTTTGTGATCATGGCGCGCACAGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCCGGCCTa                          >  2:1534344/1‑150 (MQ=255)

                                                                                                                          aTAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATg                        <  2:3418770/150‑1 (MQ=255)

                                                                                                                          aTAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATg                        >  2:1829188/1‑150 (MQ=255)

                                                                                                                            aaaGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGtt                      >  2:1199443/1‑150 (MQ=255)

                                                                                                                              aGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGa                    >  2:4082468/1‑150 (MQ=255)

                                                                                                                                cGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAg                  <  2:2166157/150‑1 (MQ=255)

                                                                                                                                 gATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGc                 >  1:1368412/1‑150 (MQ=255)

                                                                                                                                  aTCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCg                >  2:3815128/1‑150 (MQ=255)

                                                                                                                                     gTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGt             <  1:2148447/150‑1 (MQ=255)

                                                                                                                                      tctcGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTg            >  1:2451044/1‑150 (MQ=255)

                                                                                                                                          gAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCga        >  1:1939163/1‑150 (MQ=255)

                                                                                                                                           aaaGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCgag       >  2:3175810/1‑150 (MQ=255)

                                                                                                                                            aagaagATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCgaga      >  2:1032534/1‑150 (MQ=255)

                                                                                                                                             agaagaTATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGAt     <  1:2834630/150‑1 (MQ=255)

TATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTT  >  CP009273/344653‑344946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGT  >  CP009273/344652‑344948

ATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATA                                                                                                                                                     >  SRR19973079.2040918/1‑150 (MQ=60)

ATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATA                                                                                                                                                     <  SRR19973079.3714651/150‑1 (MQ=60)

 TATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATAT                                                                                                                                                    >  SRR19973079.3716758/1‑150 (MQ=60)

  ATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATG                                                                                                                                                   <  SRR19973079.1887622/150‑1 (MQ=60)

  ATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATG                                                                                                                                                   <  SRR19973079.2673438/150‑1 (MQ=60)

  ATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATG                                                                                                                                                   <  SRR19973079.488664/150‑1 (MQ=60)

   TCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGG                                                                                                                                                  <  SRR19973079.192312/150‑1 (MQ=60)

   TCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGG                                                                                                                                                  <  SRR19973079.2377224/150‑1 (MQ=60)

   TCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGG                                                                                                                                                  <  SRR19973079.2377216/150‑1 (MQ=60)

     GGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTG                                                                                                                                                >  SRR19973079.2281738/1‑150 (MQ=60)

     GGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTG                                                                                                                                                >  SRR19973079.3961854/1‑150 (MQ=60)

     GGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTG                                                                                                                                                >  SRR19973079.3961220/1‑150 (MQ=60)

      GTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGG                                                                                                                                               <  SRR19973079.1463787/150‑1 (MQ=60)

      GTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGG                                                                                                                                               >  SRR19973079.2320881/1‑150 (MQ=60)

        TTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGAT                                                                                                                                             >  SRR19973079.1184816/1‑150 (MQ=60)

        TTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGAT                                                                                                                                             >  SRR19973079.1184829/1‑150 (MQ=60)

          TGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCG                                                                                                                                           <  SRR19973079.3440151/150‑1 (MQ=60)

           GGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGG                                                                                                                                          >  SRR19973079.3455038/1‑150 (MQ=60)

             TTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGAT                                                                                                                                        <  SRR19973079.1654258/150‑1 (MQ=60)

             TTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGAT                                                                                                                                        <  SRR19973079.1654266/150‑1 (MQ=60)

               CTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCC                                                                                                                                      <  SRR19973079.1572909/150‑1 (MQ=60)

                 TCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGC                                                                                                                                    <  SRR19973079.3184604/150‑1 (MQ=60)

                 TCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGC                                                                                                                                    <  SRR19973079.3183255/150‑1 (MQ=60)

                  CGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCG                                                                                                                                   <  SRR19973079.3168873/150‑1 (MQ=60)

                  CGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCG                                                                                                                                   <  SRR19973079.3168916/150‑1 (MQ=60)

                    GCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCG                                                                                                                                 >  SRR19973079.2934972/1‑150 (MQ=60)

                    GCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCG                                                                                                                                 >  SRR19973079.3098952/1‑150 (MQ=60)

                      CTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGC                                                                                                                               >  SRR19973079.3436424/1‑150 (MQ=60)

                          AACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTG                                                                                                                           <  SRR19973079.2974756/150‑1 (MQ=60)

                          AACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTG                                                                                                                           <  SRR19973079.2974830/150‑1 (MQ=60)

                            CGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGA                                                                                                                         <  SRR19973079.71956/150‑1 (MQ=60)

                             GTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCTGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGAT                                                                                                                        <  SRR19973079.372911/150‑1 (MQ=60)

                             GTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGAT                                                                                                                        <  SRR19973079.3538944/150‑1 (MQ=60)

                              TGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATG                                                                                                                       >  SRR19973079.167370/1‑150 (MQ=60)

                              TGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATG                                                                                                                       <  SRR19973079.1693920/150‑1 (MQ=60)

                                  GCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAA                                                                                                                   <  SRR19973079.281080/150‑1 (MQ=60)

                                  GCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAA                                                                                                                   <  SRR19973079.280078/150‑1 (MQ=60)

                                   CGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAA                                                                                                                  <  SRR19973079.1748569/150‑1 (MQ=60)

                                     CACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAAC                                                                                                                <  SRR19973079.2682631/150‑1 (MQ=60)

                                     CACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAAC                                                                                                                >  SRR19973079.2430524/1‑150 (MQ=60)

                                     CACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAAC                                                                                                                <  SRR19973079.2682634/150‑1 (MQ=60)

                                           GAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCC                                                                                                          >  SRR19973079.202499/1‑150 (MQ=60)

                                             AATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTG                                                                                                        <  SRR19973079.2365891/150‑1 (MQ=60)

                                             AATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTG                                                                                                        <  SRR19973079.2924532/150‑1 (MQ=60)

                                              ATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGA                                                                                                       >  SRR19973079.2976871/1‑150 (MQ=60)

                                              ATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGA                                                                                                       <  SRR19973079.2682942/150‑1 (MQ=60)

                                              ATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGA                                                                                                       <  SRR19973079.2684617/150‑1 (MQ=60)

                                              ATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGA                                                                                                       >  SRR19973079.2976893/1‑150 (MQ=60)

                                              ATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGA                                                                                                       >  SRR19973079.334641/1‑150 (MQ=60)

                                              ATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGA                                                                                                       >  SRR19973079.2079124/1‑150 (MQ=60)

                                                CGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATT                                                                                                     <  SRR19973079.3708772/150‑1 (MQ=60)

                                                 GATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTT                                                                                                    <  SRR19973079.3662560/150‑1 (MQ=60)

                                                 GATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTT                                                                                                    <  SRR19973079.4273132/150‑1 (MQ=60)

                                                 GATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTT                                                                                                    >  SRR19973079.2399227/1‑150 (MQ=60)

                                                 GATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTT                                                                                                    <  SRR19973079.4273107/150‑1 (MQ=60)

                                                 GATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTT                                                                                                    <  SRR19973079.4273092/150‑1 (MQ=60)

                                                  ATGATTAAAGCCGGTGCTGAAGGTTTGCATATTGAAGATCTGGTTGGTGCGAATCGCTGCTGTCATCGTCCGAATATAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTT                                                                                                   <  SRR19973079.384398/150‑1 (MQ=60)

                                                  ATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTT                                                                                                   <  SRR19973079.2279240/150‑1 (MQ=60)

                                                  ATGATTAAAGCCGGTGCGGCAGGAGTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTT                                                                                                   <  SRR19973079.2279594/150‑1 (MQ=60)

                                                       TAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGAT                                                                                              <  SRR19973079.4148201/150‑1 (MQ=60)

                                                        AAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATC                                                                                             <  SRR19973079.2668051/150‑1 (MQ=60)

                                                        AAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATC                                                                                             <  SRR19973079.2696988/150‑1 (MQ=60)

                                                        AAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATC                                                                                             <  SRR19973079.2696978/150‑1 (MQ=60)

                                                         AAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCA                                                                                            >  SRR19973079.4122700/1‑150 (MQ=60)

                                                              GGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCG                                                                                       >  SRR19973079.1428832/1‑150 (MQ=60)

                                                                TGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCG                                                                                     <  SRR19973079.282077/150‑1 (MQ=60)

                                                                TGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCG                                                                                     <  SRR19973079.4130017/150‑1 (MQ=60)

                                                                   GGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCAC                                                                                  >  SRR19973079.186000/1‑150 (MQ=60)

                                                                         ATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGC                                                                            >  SRR19973079.861138/1‑150 (MQ=60)

                                                                           TGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTC                                                                          >  SRR19973079.1969753/1‑150 (MQ=60)

                                                                            GCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCT                                                                         >  SRR19973079.2255868/1‑150 (MQ=60)

                                                                            GCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCT                                                                         <  SRR19973079.2624838/150‑1 (MQ=60)

                                                                             CATATTGAAGATCAGGTTGGTGCTAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTG                                                                        >  SRR19973079.2876844/1‑150 (MQ=60)

                                                                             CATATTGAAGATCAGGTTGGTGCTAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTG                                                                        >  SRR19973079.2874999/1‑150 (MQ=60)

                                                                              ATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGG                                                                       >  SRR19973079.3431079/1‑150 (MQ=60)

                                                                               TATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGC                                                                      <  SRR19973079.1049886/150‑1 (MQ=60)

                                                                                  TGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGT                                                                   <  SRR19973079.2575300/150‑1 (MQ=60)

                                                                                     AGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGA                                                                >  SRR19973079.3530734/1‑150 (MQ=60)

                                                                                            GTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTG                                                         <  SRR19973079.644967/150‑1 (MQ=60)

                                                                                             TTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGG                                                        <  SRR19973079.1003305/150‑1 (MQ=60)

                                                                                             TTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGG                                                        <  SRR19973079.1297759/150‑1 (MQ=60)

                                                                                             TTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGG                                                        <  SRR19973079.482557/150‑1 (MQ=60)

                                                                                              TGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGA                                                       >  SRR19973079.1376227/1‑150 (MQ=60)

                                                                                                GTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATG                                                     <  SRR19973079.3970970/150‑1 (MQ=60)

                                                                                                   CGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGG                                                  <  SRR19973079.2299101/150‑1 (MQ=60)

                                                                                                    GAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGC                                                 <  SRR19973079.4124916/150‑1 (MQ=60)

                                                                                                    GAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGC                                                 <  SRR19973079.3660793/150‑1 (MQ=60)

                                                                                                      AACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGA                                               <  SRR19973079.1989240/150‑1 (MQ=60)

                                                                                                       ACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTTGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGAT                                              <  SRR19973079.3829776/150‑1 (MQ=60)

                                                                                                        CGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATC                                             <  SRR19973079.2437926/150‑1 (MQ=60)

                                                                                                         GCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCG                                            >  SRR19973079.3032333/1‑150 (MQ=60)

                                                                                                          CTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGA                                           >  SRR19973079.1799045/1‑150 (MQ=60)

                                                                                                              GGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGT                                       <  SRR19973079.2298782/150‑1 (MQ=60)

                                                                                                               GTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTG                                      <  SRR19973079.540545/150‑1 (MQ=60)

                                                                                                               GTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTG                                      <  SRR19973079.4167860/150‑1 (MQ=60)

                                                                                                               GTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTG                                      <  SRR19973079.1835651/150‑1 (MQ=60)

                                                                                                                    CGTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAG                                 <  SRR19973079.3884103/150‑1 (MQ=60)

                                                                                                                     GTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGG                                <  SRR19973079.648361/150‑1 (MQ=60)

                                                                                                                     GTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGG                                <  SRR19973079.1275352/150‑1 (MQ=60)

                                                                                                                     GTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGG                                >  SRR19973079.1677107/1‑150 (MQ=60)

                                                                                                                     GTCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGG                                <  SRR19973079.3477741/150‑1 (MQ=60)

                                                                                                                      TCCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGC                               >  SRR19973079.1503004/1‑150 (MQ=60)

                                                                                                                       CCGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCC                              >  SRR19973079.431063/1‑150 (MQ=60)

                                                                                                                        CGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCT                             >  SRR19973079.2611104/1‑150 (MQ=60)

                                                                                                                        CGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCT                             >  SRR19973079.535687/1‑150 (MQ=60)

                                                                                                                        CGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCT                             >  SRR19973079.3104488/1‑150 (MQ=60)

                                                                                                                        CGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCT                             >  SRR19973079.534454/1‑150 (MQ=60)

                                                                                                                        CGAATAAAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCT                             <  SRR19973079.487790/150‑1 (MQ=60)

                                                                                                                          AATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTAT                           <  SRR19973079.4316325/150‑1 (MQ=60)

                                                                                                                              AAGCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTG                       >  SRR19973079.4285895/1‑150 (MQ=60)

                                                                                                                                GCGATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAA                     <  SRR19973079.3396316/150‑1 (MQ=60)

                                                                                                                                  GATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGC                   >  SRR19973079.1375823/1‑150 (MQ=60)

                                                                                                                                  GATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGC                   >  SRR19973079.2398392/1‑150 (MQ=60)

                                                                                                                                  GATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGC                   <  SRR19973079.808581/150‑1 (MQ=60)

                                                                                                                                  GATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGC                   >  SRR19973079.1375825/1‑150 (MQ=60)

                                                                                                                                  GATCGTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGC                   >  SRR19973079.1376006/1‑150 (MQ=60)

                                                                                                                                      GTCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGT               <  SRR19973079.2159479/150‑1 (MQ=60)

                                                                                                                                       TCTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTG              >  SRR19973079.2463617/1‑150 (MQ=60)

                                                                                                                                        CTCGAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGC             >  SRR19973079.3249438/1‑150 (MQ=60)

                                                                                                                                           GAAAGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGA          >  SRR19973079.1949501/1‑150 (MQ=60)

                                                                                                                                             AAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGA        >  SRR19973079.837407/1‑150 (MQ=60)

                                                                                                                                              AGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGAT       <  SRR19973079.2849091/150‑1 (MQ=60)

                                                                                                                                              AGAAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGAT       <  SRR19973079.2850597/150‑1 (MQ=60)

                                                                                                                                                AAGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGT     >  SRR19973079.1440276/1‑150 (MQ=60)

                                                                                                                                                AAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGT     >  SRR19973079.1686043/1‑150 (MQ=60)

                                                                                                                                                 AGATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTT    <  SRR19973079.3130891/150‑1 (MQ=60)

                                                                                                                                                  GATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTG   <  SRR19973079.4305723/150‑1 (MQ=60)

                                                                                                                                                  GATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTG   <  SRR19973079.4305672/150‑1 (MQ=60)

                                                                                                                                                  GATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTG   <  SRR19973079.4250496/150‑1 (MQ=60)

                                                                                                                                                   ATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGT  <  SRR19973079.758664/150‑1 (MQ=60)

                                                                                                                                                   ATATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGT  >  SRR19973079.2748308/1‑150 (MQ=60)

ATATCGGTTTTGGTTCTTCGGCCTTTAACGTGGCGCGCACCGTGAAATCGATGATTAAAGCCGGTGCGGCAGGATTGCATATTGAAGATCAGGTTGGTGCGAAACGCTGCGGTCATCGTCCGAATAAAGCGATCGTCTCGAAAGAAGAGATGGTGGATCGGATCCGCGCGGCGGTGGATGCGAAAACCGATCCTGATTTTGTGATCATGGCGCGCACCGATGCTCTGGCGGTAGAGGGGCTGGATGCGGCGATCGAGCGTGCGCAGGCCTATGTTGAAGCGGGTGCCGAGATGTTGT  >  CP009273/344652‑344948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`