Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 662,108 A→T 100% V522V (GTT→GTA)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273662,1080AT100.0% 688.7 / NA 205V522V (GTT→GTA) mrdAtranspeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (125/80);  total (125/80)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

CGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCA  >  CP009273/661968‑662252
                                                                                                                                            |                                                                                                                                                
cGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGt                                                                                                                                           <  2:199908/148‑1 (MQ=255)
 gTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGtt                                                                                                                                          <  2:1081903/148‑2 (MQ=255)
     aCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATa                                                                                                                                      <  2:2627711/148‑1 (MQ=255)
         tCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGt                                                                                                                                  <  2:920447/148‑1 (MQ=255)
         tCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGt                                                                                                                                  >  2:1576505/1‑148 (MQ=255)
         tCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGt                                                                                                                                  <  1:1251588/148‑1 (MQ=255)
            gCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCtt                                                                                                                               >  1:1011912/1‑148 (MQ=255)
             cAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCttt                                                                                                                              >  1:2759332/1‑148 (MQ=255)
             cAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCttt                                                                                                                              >  2:1837834/1‑148 (MQ=255)
               aTTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCg                                                                                                                            <  1:1911070/148‑1 (MQ=255)
                ttttGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGc                                                                                                                           >  1:2965062/1‑148 (MQ=255)
                 tttGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGcc                                                                                                                          >  2:2154738/1‑148 (MQ=255)
                 tttGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGcc                                                                                                                          >  2:680267/1‑148 (MQ=255)
                          aTTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAg                                                                                                                 <  2:1973541/148‑1 (MQ=255)
                          aTTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAg                                                                                                                 <  2:3212047/148‑1 (MQ=255)
                           ttATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGt                                                                                                                >  2:2238747/1‑148 (MQ=255)
                           ttATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGt                                                                                                                >  2:1725903/1‑148 (MQ=255)
                            tataGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTa                                                                                                               >  2:2124761/1‑148 (MQ=255)
                            tataGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTa                                                                                                               >  2:1938333/1‑148 (MQ=255)
                             ataGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTaa                                                                                                              >  1:3066665/1‑148 (MQ=255)
                              taGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAAc                                                                                                             >  2:436477/1‑148 (MQ=255)
                               aGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAAcc                                                                                                            >  1:365333/1‑148 (MQ=255)
                                ggTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCg                                                                                                           >  1:310800/1‑148 (MQ=255)
                                 gTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCgg                                                                                                          >  1:795545/1‑148 (MQ=255)
                                  tttCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGTGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGga                                                                                                         <  2:416196/148‑1 (MQ=255)
                                   ttcgttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaa                                                                                                        >  2:805635/1‑148 (MQ=255)
                                   ttcgttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaa                                                                                                        >  2:1323418/1‑148 (MQ=255)
                                   ttcgttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaa                                                                                                        <  1:2967829/148‑1 (MQ=255)
                                      gttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatga                                                                                                     <  1:1672906/148‑1 (MQ=255)
                                        tcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatgaat                                                                                                   >  2:1972967/1‑148 (MQ=255)
                                        tcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatgaat                                                                                                   <  1:1515394/148‑1 (MQ=255)
                                        tcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatgaat                                                                                                   <  1:1512992/148‑1 (MQ=255)
                                        tcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatgaat                                                                                                   <  1:3026648/148‑1 (MQ=255)
                                         cgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAata                                                                                                  <  1:1435918/148‑1 (MQ=255)
                                         cgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAata                                                                                                  <  2:2556126/148‑1 (MQ=255)
                                         cgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAata                                                                                                  <  2:3204693/148‑1 (MQ=255)
                                         cgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAata                                                                                                  <  2:679909/148‑1 (MQ=255)
                                          gCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAatat                                                                                                 >  2:1515486/1‑148 (MQ=255)
                                          gCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAatat                                                                                                 >  1:2326439/1‑148 (MQ=255)
                                            tttCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCg                                                                                               >  1:1023268/1‑148 (MQ=255)
                                             ttCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGc                                                                                              >  1:1854726/1‑148 (MQ=255)
                                                  aCCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACg                                                                                         <  2:1709231/148‑1 (MQ=255)
                                                  aCCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTAAATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACg                                                                                         >  1:655928/1‑148 (MQ=255)
                                                  aCCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTAACGTTAGGGCGGTTAGCTACACCGTACATACCGTATTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACg                                                                                         >  1:3156642/1‑148 (MQ=255)
                                                    cGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACggg                                                                                       <  1:244355/148‑1 (MQ=255)
                                                     gAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGc                                                                                      >  2:2950850/1‑148 (MQ=255)
                                                     gAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGc                                                                                      >  1:1875328/1‑148 (MQ=255)
                                                      aaGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCg                                                                                     >  1:1902604/1‑148 (MQ=255)
                                                      aaGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCg                                                                                     >  1:1893892/1‑148 (MQ=255)
                                                       aGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCgg                                                                                    <  2:2511886/148‑1 (MQ=255)
                                                             gAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATg                                                                              >  1:1043896/1‑148 (MQ=255)
                                                             gAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATg                                                                              <  1:329177/148‑1 (MQ=255)
                                                              aGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGc                                                                             >  2:774435/1‑148 (MQ=255)
                                                                        gATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTAccc                                                                   >  2:1830849/1‑148 (MQ=255)
                                                                              gccgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCa                                                             >  2:760155/1‑148 (MQ=255)
                                                                               ccgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCAc                                                            <  1:2597885/148‑1 (MQ=255)
                                                                                cgcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCAcc                                                           >  1:651810/1‑148 (MQ=255)
                                                                                 gcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCt                                                          <  1:680247/148‑1 (MQ=255)
                                                                                 gcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCt                                                          >  2:2656023/1‑148 (MQ=255)
                                                                                 gcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCt                                                          <  1:2154738/148‑1 (MQ=255)
                                                                                  cAATTTTGTACGGTGCTCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTg                                                         <  2:1405770/148‑1 (MQ=255)
                                                                                    aTTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGtt                                                       >  1:765760/1‑148 (MQ=255)
                                                                                    aTTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGtt                                                       >  2:2675206/1‑148 (MQ=255)
                                                                                     ttttGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGttt                                                      >  1:857987/1‑148 (MQ=255)
                                                                                     ttttGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGttt                                                      >  1:1596985/1‑148 (MQ=255)
                                                                                      tttGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTg                                                     >  1:2534617/1‑148 (MQ=255)
                                                                                      tttGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTg                                                     <  1:287449/148‑1 (MQ=255)
                                                                                          tACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGt                                                 <  1:543390/148‑1 (MQ=255)
                                                                                            cGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCt                                               <  1:1744849/148‑1 (MQ=255)
                                                                                               tGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTcg                                            >  2:2200620/1‑148 (MQ=255)
                                                                                               tGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTcg                                            >  2:2202954/1‑148 (MQ=255)
                                                                                               tGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTcg                                            >  2:537846/1‑148 (MQ=255)
                                                                                                     aGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTg                                      >  1:169328/1‑148 (MQ=255)
                                                                                                     aGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTg                                      >  1:168992/1‑148 (MQ=255)
                                                                                                      gCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGc                                     >  1:1977965/1‑148 (MQ=255)
                                                                                                      gCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGc                                     >  1:109736/1‑148 (MQ=255)
                                                                                                       cAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCt                                    >  1:2452508/1‑148 (MQ=255)
                                                                                                       cAAAGTATTTATGCGCCGTACCGTAAGGGCGGTAAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGAAACCGGAATGAATATCGCCGACGCCCGGTTCAAGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCTGCGGTGCt                                    >  1:2995345/1‑148 (MQ=255)
                                                                                                         aaGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtca                                  <  2:2326439/148‑1 (MQ=255)
                                                                                                         aaGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtca                                  >  1:1162131/1‑148 (MQ=255)
                                                                                                            tATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcatca                               >  2:1826277/1‑148 (MQ=255)
                                                                                                             aTTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAg                              >  1:2891596/1‑148 (MQ=255)
                                                                                                             aTTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAg                              >  2:2886078/1‑148 (MQ=255)
                                                                                                                tATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCaa                           <  2:28039/148‑1 (MQ=255)
                                                                                                                tATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCaa                           <  2:867651/148‑1 (MQ=255)
                                                                                                                  tGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAAt                         >  1:530622/1‑148 (MQ=255)
                                                                                                                   gcgcCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATg                        <  2:1591339/148‑1 (MQ=255)
                                                                                                                      ccGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAgg                     >  1:597464/1‑148 (MQ=255)
                                                                                                                       cGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGa                    <  1:2889138/148‑1 (MQ=255)
                                                                                                                        gTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGaa                   <  2:3087342/148‑1 (MQ=255)
                                                                                                                         tACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAAc                  >  1:871457/1‑148 (MQ=255)
                                                                                                                          aCCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAAcc                 <  2:652774/148‑1 (MQ=255)
                                                                                                                          aCCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAAcc                 <  2:655928/148‑1 (MQ=255)
                                                                                                                            cGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCtt               <  2:3284821/148‑1 (MQ=255)
                                                                                                                             gTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTc              >  1:962516/1‑148 (MQ=255)
                                                                                                                             gTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTc              >  1:2621474/1‑148 (MQ=255)
                                                                                                                              ttAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCa             <  1:1837834/148‑1 (MQ=255)
                                                                                                                                 gggCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGa          <  1:3220926/148‑1 (MQ=255)
                                                                                                                                      gTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCg     >  2:1854846/1‑148 (MQ=255)
                                                                                                                                         aGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAAGATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCa  <  1:427553/148‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                
CGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCA  >  CP009273/661968‑662252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAAT  >  CP009273/661960‑662257
                                                                                                                                                    |                                                                                                                                                     
TGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTA                                                                                                                                                      <  SRR19973070.3288967/150‑1 (MQ=60)
TGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTA                                                                                                                                                      <  SRR19973070.2335211/150‑1 (MQ=60)
         GTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACA                                                                                                                                             >  SRR19973070.618698/1‑150 (MQ=60)
         GTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACA                                                                                                                                             >  SRR19973070.374106/1‑150 (MQ=60)
          TAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACAT                                                                                                                                            <  SRR19973070.1535803/150‑1 (MQ=60)
                 TCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCNATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCT                                                                                                                                     <  SRR19973070.1692045/150‑1 (MQ=60)
                 TCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCT                                                                                                                                     <  SRR19973070.3090661/150‑1 (MQ=60)
                 TCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCT                                                                                                                                     <  SRR19973070.1260368/150‑1 (MQ=60)
                  CGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTT                                                                                                                                    >  SRR19973070.1019966/1‑150 (MQ=60)
                  CGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTT                                                                                                                                    <  SRR19973070.3054100/150‑1 (MQ=60)
                  CGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTT                                                                                                                                    >  SRR19973070.1019105/1‑150 (MQ=60)
                   GGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTT                                                                                                                                   >  SRR19973070.2777033/1‑150 (MQ=60)
                     CAATTTTGAGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACATGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACAGTTAGGGCGGTTAGCTACACCGTACATACAGTCTTTCT                                                                                                                                 >  SRR19973070.2566697/1‑150 (MQ=60)
                      AATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGC                                                                                                                                >  SRR19973070.2984126/1‑150 (MQ=60)
                       ATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCC                                                                                                                               <  SRR19973070.1923982/150‑1 (MQ=60)
                         TTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAG                                                                                                                             <  SRR19973070.2107147/150‑1 (MQ=60)
                              GCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCC                                                                                                                        <  SRR19973070.2938129/150‑1 (MQ=60)
                              GCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCC                                                                                                                        <  SRR19973070.998384/150‑1 (MQ=60)
                                GCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAG                                                                                                                      <  SRR19973070.1638682/150‑1 (MQ=60)
                                   TTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAA                                                                                                                   >  SRR19973070.3086326/1‑150 (MQ=60)
                                    TATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAAC                                                                                                                  >  SRR19973070.1704396/1‑150 (MQ=60)
                                     ATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACC                                                                                                                 >  SRR19973070.367978/1‑150 (MQ=60)
                                     ATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACC                                                                                                                 >  SRR19973070.368013/1‑150 (MQ=60)
                                      TAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCG                                                                                                                >  SRR19973070.577400/1‑150 (MQ=60)
                                      TAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCG                                                                                                                >  SRR19973070.313027/1‑150 (MQ=60)
                                       AGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGG                                                                                                               >  SRR19973070.801040/1‑150 (MQ=60)
                                           TTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATG                                                                                                           <  SRR19973070.2986900/150‑1 (MQ=60)
                                           TTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATG                                                                                                           <  SRR19973070.2985905/150‑1 (MQ=60)
                                              GTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAAT                                                                                                        <  SRR19973070.1684372/150‑1 (MQ=60)
                                                TCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATAT                                                                                                      >  SRR19973070.671323/1‑150 (MQ=60)
                                                TCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATAT                                                                                                      <  SRR19973070.1526013/150‑1 (MQ=60)
                                                TCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATAT                                                                                                      <  SRR19973070.3045924/150‑1 (MQ=60)
                                                TCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATAT                                                                                                      >  SRR19973070.2341896/1‑150 (MQ=60)
                                                TCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATAT                                                                                                      <  SRR19973070.1523577/150‑1 (MQ=60)
                                                TCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATAT                                                                                                      <  SRR19973070.1526009/150‑1 (MQ=60)
                                                 CGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATC                                                                                                     <  SRR19973070.1445859/150‑1 (MQ=60)
                                                 CGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATC                                                                                                     <  SRR19973070.1985485/150‑1 (MQ=60)
                                                  GCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCG                                                                                                    >  SRR19973070.1030940/1‑150 (MQ=60)
                                                  GCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCG                                                                                                    >  SRR19973070.1030525/1‑150 (MQ=60)
                                                   CTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGC                                                                                                   >  SRR19973070.1867444/1‑150 (MQ=60)
                                                   CTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGC                                                                                                   >  SRR19973070.1867427/1‑150 (MQ=60)
                                                        AGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACG                                                                                              >  SRR19973070.657429/1‑150 (MQ=60)
                                                        AGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTAAATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCTCCGACG                                                                                              >  SRR19973070.660603/1‑150 (MQ=60)
                                                        AGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTAACGTTAGGGCGGTTAGCTACACCGTACATACCGTATTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACG                                                                                              >  SRR19973070.3177046/1‑150 (MQ=60)
                                                          ACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGG                                                                                            >  SRR19973070.2255717/1‑150 (MQ=60)
                                                           CCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGC                                                                                           >  SRR19973070.1888104/1‑150 (MQ=60)
                                                            CGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCG                                                                                          >  SRR19973070.1906302/1‑150 (MQ=60)
                                                            CGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCG                                                                                          >  SRR19973070.1906730/1‑150 (MQ=60)
                                                            CGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCG                                                                                          <  SRR19973070.246151/150‑1 (MQ=60)
                                                            CGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCG                                                                                          <  SRR19973070.246036/150‑1 (MQ=60)
                                                            CGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCG                                                                                          >  SRR19973070.1915724/1‑150 (MQ=60)
                                                            CGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCG                                                                                          >  SRR19973070.1906732/1‑150 (MQ=60)
                                                            CGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCG                                                                                          >  SRR19973070.1915476/1‑150 (MQ=60)
                                                              AAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGT                                                                                        >  SRR19973070.3007686/1‑150 (MQ=60)
                                                              AAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGT                                                                                        >  SRR19973070.3226096/1‑150 (MQ=60)
                                                                 ACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCA                                                                                     >  SRR19973070.2662246/1‑150 (MQ=60)
                                                                   CTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATG                                                                                   >  SRR19973070.1051256/1‑150 (MQ=60)
                                                                     GAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCG                                                                                 <  SRR19973070.331584/150‑1 (MQ=60)
                                                                            ACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGTGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTAC                                                                          <  SRR19973070.1706864/150‑1 (MQ=60)
                                                                                      GCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACC                                                                >  SRR19973070.656461/1‑150 (MQ=60)
                                                                                       CCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCT                                                               <  SRR19973070.2614855/150‑1 (MQ=60)
                                                                                        CGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTG                                                              >  SRR19973070.3274264/1‑150 (MQ=60)
                                                                                         GCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGT                                                             <  SRR19973070.685123/150‑1 (MQ=60)
                                                                                         GCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGT                                                             <  SRR19973070.2169074/150‑1 (MQ=60)
                                                                                         GCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGT                                                             <  SRR19973070.685103/150‑1 (MQ=60)
                                                                                          CAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTT                                                            <  SRR19973070.3020036/150‑1 (MQ=60)
                                                                                          CAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTT                                                            >  SRR19973070.771140/1‑150 (MQ=60)
                                                                                           AATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTT                                                           >  SRR19973070.863922/1‑150 (MQ=60)
                                                                                           AATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTT                                                           >  SRR19973070.863924/1‑150 (MQ=60)
                                                                                           AATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTT                                                           >  SRR19973070.1608144/1‑150 (MQ=60)
                                                                                            ATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTG                                                          >  SRR19973070.2551182/1‑150 (MQ=60)
                                                                                              TTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCC                                                        <  SRR19973070.289380/150‑1 (MQ=60)
                                                                                                  TACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCT                                                    <  SRR19973070.263559/150‑1 (MQ=60)
                                                                                                  TACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCT                                                    <  SRR19973070.547014/150‑1 (MQ=60)
                                                                                                  TACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCT                                                    >  SRR19973070.457273/1‑150 (MQ=60)
                                                                                                  TACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCT                                                    <  SRR19973070.3283936/150‑1 (MQ=60)
                                                                                                    CGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTC                                                  <  SRR19973070.1756947/150‑1 (MQ=60)
                                                                                                           CTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTG                                           >  SRR19973070.170698/1‑150 (MQ=60)
                                                                                                           CTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTG                                           >  SRR19973070.170361/1‑150 (MQ=60)
                                                                                                            TAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGC                                          >  SRR19973070.1991336/1‑150 (MQ=60)
                                                                                                            TAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGC                                          >  SRR19973070.111590/1‑150 (MQ=60)
                                                                                                            TAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGC                                          >  SRR19973070.110752/1‑150 (MQ=60)
                                                                                                             AGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCT                                         >  SRR19973070.2468550/1‑150 (MQ=60)
                                                                                                             AGCAAAGTATTTATGCGCCGTACCGTAAGGGCGGTAAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGAAACCGGAATGAATATCGCCGACGCCCGGTTCAAGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCTGCGGTGCT                                         >  SRR19973070.3014522/1‑150 (MQ=60)
                                                                                                             AGCAAAGTATTTATGCGCCGTACCGATAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCT                                         >  SRR19973070.800664/1‑150 (MQ=60)
                                                                                                               CAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCA                                       >  SRR19973070.1170264/1‑150 (MQ=60)
                                                                                                               CAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCA                                       >  SRR19973070.1425015/1‑150 (MQ=60)
                                                                                                                   GTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAG                                   >  SRR19973070.2910299/1‑150 (MQ=60)
                                                                                                                   GTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAG                                   >  SRR19973070.2910310/1‑150 (MQ=60)
                                                                                                                    TATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGC                                  >  SRR19973070.3162841/1‑150 (MQ=60)
                                                                                                                        TATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAAT                              >  SRR19973070.534203/1‑150 (MQ=60)
                                                                                                                        TATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACAGACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAAT                              >  SRR19973070.534204/1‑150 (MQ=60)
                                                                                                                         ATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATG                             <  SRR19973070.2397640/150‑1 (MQ=60)
                                                                                                                            CGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGG                          >  SRR19973070.866369/1‑150 (MQ=60)
                                                                                                                            CGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGG                          >  SRR19973070.601617/1‑150 (MQ=60)
                                                                                                                              CCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAA                        <  SRR19973070.1861375/150‑1 (MQ=60)
                                                                                                                               CGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAAC                       <  SRR19973070.2907841/150‑1 (MQ=60)
                                                                                                                               CGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAAC                       >  SRR19973070.877561/1‑150 (MQ=60)
                                                                                                                               CGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAAC                       <  SRR19973070.2907829/150‑1 (MQ=60)
                                                                                                                                GTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACC                      <  SRR19973070.1951362/150‑1 (MQ=60)
                                                                                                                                  ACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTT                    >  SRR19973070.1074526/1‑150 (MQ=60)
                                                                                                                                   CCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTC                   >  SRR19973070.2638578/1‑150 (MQ=60)
                                                                                                                                   CCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTC                   >  SRR19973070.969386/1‑150 (MQ=60)
                                                                                                                                      TTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACG                <  SRR19973070.1850443/150‑1 (MQ=60)
                                                                                                                                      TTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACG                <  SRR19973070.1850455/150‑1 (MQ=60)
                                                                                                                                       TAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGA               >  SRR19973070.2473197/1‑150 (MQ=60)
                                                                                                                                       TAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGA               >  SRR19973070.934697/1‑150 (MQ=60)
                                                                                                                                       TAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGA               >  SRR19973070.1183023/1‑150 (MQ=60)
                                                                                                                                         GGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATA             <  SRR19973070.3241741/150‑1 (MQ=60)
                                                                                                                                            CGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCG          >  SRR19973070.1449489/1‑150 (MQ=60)
                                                                                                                                             GGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGT         >  SRR19973070.2880465/1‑150 (MQ=60)
                                                                                                                                                 AGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAAGATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATT     <  SRR19973070.430671/150‑1 (MQ=60)
                                                                                                                                                    TACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAAT  <  SRR19973070.2419864/150‑1 (MQ=60)
                                                                                                                                                    TACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAAT  >  SRR19973070.727611/1‑150 (MQ=60)
                                                                                                                                                    TACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAAT  <  SRR19973070.2165477/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAAT  >  CP009273/661960‑662257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑