Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 662,108 A→T 100% V522V (GTT→GTA)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273662,1080AT100.0% 772.2 / NA 229V522V (GTT→GTA) mrdAtranspeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (129/100);  total (129/100)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 229 total aligned reads displayed.

GGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGT  >  CP009273/661963‑662250
                                                                                                                                                 |                                                                                                                                              
     cGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGt                                                                                                                                         >  2:1080971/1‑148 (MQ=255)
       tAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTAc                                                                                                                                       <  2:854183/148‑1 (MQ=255)
       tAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTAc                                                                                                                                       <  2:2664159/148‑1 (MQ=255)
       tAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTAc                                                                                                                                       <  2:1334369/148‑1 (MQ=255)
        aaaCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACa                                                                                                                                      >  2:3636393/1‑148 (MQ=255)
         aaCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACAt                                                                                                                                     >  1:1667570/1‑148 (MQ=255)
         aaCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACAt                                                                                                                                     <  1:1893734/148‑1 (MQ=255)
          aCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATa                                                                                                                                    <  2:3547287/148‑1 (MQ=255)
          aCGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATa                                                                                                                                    >  1:3492002/1‑148 (MQ=255)
           cGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATAc                                                                                                                                   >  1:2196844/1‑148 (MQ=255)
           cGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATAc                                                                                                                                   >  2:2410393/1‑148 (MQ=255)
             cTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCg                                                                                                                                 <  1:1053550/148‑1 (MQ=255)
             cTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCg                                                                                                                                 >  1:1980432/1‑148 (MQ=255)
               cGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTc                                                                                                                               <  1:1924972/148‑1 (MQ=255)
               cGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTc                                                                                                                               >  1:2339850/1‑148 (MQ=255)
               cGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTc                                                                                                                               <  1:2147228/148‑1 (MQ=255)
                ggCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCt                                                                                                                              <  1:2219868/148‑1 (MQ=255)
                ggCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGAGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCt                                                                                                                              <  1:3556926/148‑1 (MQ=255)
                 gCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCtt                                                                                                                             <  1:1499161/148‑1 (MQ=255)
                  cAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCttt                                                                                                                            >  1:3781748/1‑148 (MQ=255)
                  cAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCttt                                                                                                                            >  2:936909/1‑148 (MQ=255)
                     ttttGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGc                                                                                                                         >  1:3679826/1‑148 (MQ=255)
                     ttttGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGc                                                                                                                         <  2:2459781/148‑1 (MQ=255)
                      tttGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGcc                                                                                                                        <  1:1097642/148‑1 (MQ=255)
                      tttGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATATATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGcc                                                                                                                        >  2:1377003/1‑148 (MQ=255)
                            cgcATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTcc                                                                                                                  >  1:3232394/1‑148 (MQ=255)
                               aTTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAg                                                                                                               >  2:2961392/1‑148 (MQ=255)
                                ttATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGt                                                                                                              >  1:882777/1‑148 (MQ=255)
                                ttATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGt                                                                                                              <  1:2916204/148‑1 (MQ=255)
                                 tataGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTa                                                                                                             <  1:1955169/148‑1 (MQ=255)
                                       tttCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGga                                                                                                       >  1:22899/1‑148 (MQ=255)
                                       tttCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGga                                                                                                       >  2:1367797/1‑148 (MQ=255)
                                       tttCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTAGCCGCAATTTTTTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGga                                                                                                       >  1:22649/1‑148 (MQ=255)
                                        ttcgttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaa                                                                                                      >  2:3056617/1‑148 (MQ=255)
                                          cgttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatg                                                                                                    <  2:1692099/148‑1 (MQ=255)
                                           gttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatga                                                                                                   <  2:1456965/148‑1 (MQ=255)
                                            ttcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatgaa                                                                                                  <  2:3702743/148‑1 (MQ=255)
                                             tcgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGgaatgaat                                                                                                 >  1:2952533/1‑148 (MQ=255)
                                              cgCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCGAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAata                                                                                                <  2:194291/148‑1 (MQ=255)
                                               gCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAatat                                                                                               <  1:793650/148‑1 (MQ=255)
                                               gCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAatat                                                                                               <  1:1717073/148‑1 (MQ=255)
                                                  ttCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGc                                                                                            >  1:3298186/1‑148 (MQ=255)
                                                  ttCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGc                                                                                            <  2:601356/148‑1 (MQ=255)
                                                  ttCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGc                                                                                            >  1:1834847/1‑148 (MQ=255)
                                                  ttCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGc                                                                                            >  1:1052561/1‑148 (MQ=255)
                                                   tCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGcc                                                                                           >  1:2138574/1‑148 (MQ=255)
                                                    cAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCg                                                                                          <  2:128646/148‑1 (MQ=255)
                                                    cAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCg                                                                                          >  1:3312377/1‑148 (MQ=255)
                                                      gACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGAc                                                                                        >  1:513254/1‑148 (MQ=255)
                                                      gACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGAc                                                                                        <  2:3432990/148‑1 (MQ=255)
                                                       aCCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACg                                                                                       >  1:2998234/1‑148 (MQ=255)
                                                       aCCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACg                                                                                       >  2:107172/1‑148 (MQ=255)
                                                          gAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGc                                                                                    >  2:1344263/1‑148 (MQ=255)
                                                          gAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGc                                                                                    >  2:3028446/1‑148 (MQ=255)
                                                           aaGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCg                                                                                   >  1:2587825/1‑148 (MQ=255)
                                                            aGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCgg                                                                                  >  2:3809836/1‑148 (MQ=255)
                                                            aGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCgg                                                                                  >  1:2522842/1‑148 (MQ=255)
                                                             gACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGt                                                                                 <  1:3182937/148‑1 (MQ=255)
                                                              aCCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGtt                                                                                >  1:2683547/1‑148 (MQ=255)
                                                                cTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCa                                                                              <  2:3414579/148‑1 (MQ=255)
                                                                        tACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTg                                                                      >  2:2641114/1‑148 (MQ=255)
                                                                         aCCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGt                                                                     >  1:3464374/1‑148 (MQ=255)
                                                                         aCCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGt                                                                     >  1:1487600/1‑148 (MQ=255)
                                                                          ccGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTa                                                                    <  1:3633715/148‑1 (MQ=255)
                                                                          ccGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTa                                                                    <  2:2191433/148‑1 (MQ=255)
                                                                             gATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTAccc                                                                 <  1:3446370/148‑1 (MQ=255)
                                                                                ttCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATg                                                              >  1:1874675/1‑148 (MQ=255)
                                                                                      gcAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCt                                                        <  2:1980432/148‑1 (MQ=255)
                                                                                       cAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTg                                                       >  2:3243868/1‑148 (MQ=255)
                                                                                       cAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTg                                                       >  2:3489183/1‑148 (MQ=255)
                                                                                       cAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTg                                                       >  2:2560409/1‑148 (MQ=255)
                                                                                       cAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTg                                                       >  1:3672813/1‑148 (MQ=255)
                                                                                            ttGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGc                                                  >  1:60046/1‑148 (MQ=255)
                                                                                                 cGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCt                                             <  2:513807/148‑1 (MQ=255)
                                                                                                 cGGTGCGCTAGCAAAGTATTTATGAGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCt                                             <  2:718781/148‑1 (MQ=255)
                                                                                                    tGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTcg                                          <  1:25185/148‑1 (MQ=255)
                                                                                                          aGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGTCGTCTTCGGCGGTg                                    <  2:2903186/148‑1 (MQ=255)
                                                                                                            cAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCt                                  >  2:195419/1‑148 (MQ=255)
                                                                                                            cAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCt                                  >  2:1206623/1‑148 (MQ=255)
                                                                                                               aGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcat                               <  1:215961/148‑1 (MQ=255)
                                                                                                               aGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcat                               >  1:936130/1‑148 (MQ=255)
                                                                                                               aGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCAGTCTTCGGCGGTGCtcat                               >  2:3281884/1‑148 (MQ=255)
                                                                                                                gTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcatc                              <  1:733524/148‑1 (MQ=255)
                                                                                                                 tATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCtcatca                             >  2:3601532/1‑148 (MQ=255)
                                                                                                                     tATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCaa                         <  1:161422/148‑1 (MQ=255)
                                                                                                                       tGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAAt                       >  2:315300/1‑148 (MQ=255)
                                                                                                                          gcCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAg                    <  1:606054/148‑1 (MQ=255)
                                                                                                                           ccGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAgg                   >  1:2816430/1‑148 (MQ=255)
                                                                                                                             gTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGaa                 <  1:1376836/148‑1 (MQ=255)
                                                                                                                             gTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGaa                 <  1:1377007/148‑1 (MQ=255)
                                                                                                                              tACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAAc                <  1:1941709/148‑1 (MQ=255)
                                                                                                                               aCCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAAcc               <  2:3298186/148‑1 (MQ=255)
                                                                                                                                ccGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCt              >  2:843423/1‑148 (MQ=255)
                                                                                                                                  gTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTc            <  2:1667570/148‑1 (MQ=255)
                                                                                                                                    tAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCAc          >  2:1611992/1‑148 (MQ=255)
                                                                                                                                       ggCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGAt       >  1:1545946/1‑148 (MQ=255)
                                                                                                                                       ggCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGAt       >  2:1010913/1‑148 (MQ=255)
                                                                                                                                       ggCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGAt       >  1:907825/1‑148 (MQ=255)
                                                                                                                                        gCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATa      <  1:397980/148‑1 (MQ=255)
                                                                                                                                            ttAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATCTCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGt  <  1:1238624/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
GGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGT  >  CP009273/661963‑662250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAAT  >  CP009273/661959‑662257
                                                                                                                                                      |                                                                                                                                                     
TTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCT                                                                                                                                                       <  SRR19973069.2338087/150‑1 (MQ=60)
TTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCT                                                                                                                                                       <  SRR19973069.2338794/150‑1 (MQ=60)
          GTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACA                                                                                                                                             >  SRR19973069.3646028/1‑150 (MQ=60)
           TAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACAT                                                                                                                                            >  SRR19973069.1679138/1‑150 (MQ=60)
            AAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATA                                                                                                                                           >  SRR19973069.3514517/1‑150 (MQ=60)
             AACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATAC                                                                                                                                          <  SRR19973069.1906854/150‑1 (MQ=60)
             AACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATAC                                                                                                                                          >  SRR19973069.2211769/1‑150 (MQ=60)
               CGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCG                                                                                                                                        <  SRR19973069.3214622/150‑1 (MQ=60)
               CGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCG                                                                                                                                        >  SRR19973069.1994204/1‑150 (MQ=60)
                GCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGT                                                                                                                                       >  SRR19973069.595092/1‑150 (MQ=60)
                 CTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTC                                                                                                                                      <  SRR19973069.1060839/150‑1 (MQ=60)
                 CTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTC                                                                                                                                      >  SRR19973069.2355768/1‑150 (MQ=60)
                 CTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTC                                                                                                                                      <  SRR19973069.1060862/150‑1 (MQ=60)
                   CGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTT                                                                                                                                    <  SRR19973069.2161950/150‑1 (MQ=60)
                   CGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTT                                                                                                                                    <  SRR19973069.1938203/150‑1 (MQ=60)
                   CGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTT                                                                                                                                    <  SRR19973069.1937968/150‑1 (MQ=60)
                    GGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTT                                                                                                                                   <  SRR19973069.295809/150‑1 (MQ=60)
                    GGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTT                                                                                                                                   >  SRR19973069.3806294/1‑150 (MQ=60)
                    GGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTT                                                                                                                                   <  SRR19973069.2234885/150‑1 (MQ=60)
                    GGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTT                                                                                                                                   <  SRR19973069.1674333/150‑1 (MQ=60)
                    GGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGAGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTT                                                                                                                                   <  SRR19973069.3580061/150‑1 (MQ=60)
                     GCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTC                                                                                                                                  <  SRR19973069.1509553/150‑1 (MQ=60)
                      CAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTGCGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCG                                                                                                                                 <  SRR19973069.1681365/150‑1 (MQ=60)
                       AATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGC                                                                                                                                >  SRR19973069.959245/1‑150 (MQ=60)
                       AATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGC                                                                                                                                >  SRR19973069.3703892/1‑150 (MQ=60)
                          TTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAG                                                                                                                             <  SRR19973069.1105312/150‑1 (MQ=60)
                          TTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGAACGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAG                                                                                                                             <  SRR19973069.2199768/150‑1 (MQ=60)
                              TGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCC                                                                                                                         >  SRR19973069.3253546/1‑150 (MQ=60)
                              TGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCC                                                                                                                         >  SRR19973069.1498367/1‑150 (MQ=60)
                                  CATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGT                                                                                                                     >  SRR19973069.888891/1‑150 (MQ=60)
                                    TTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAA                                                                                                                   <  SRR19973069.2935501/150‑1 (MQ=60)
                                     TATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAAC                                                                                                                  <  SRR19973069.3735731/150‑1 (MQ=60)
                                     TATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAAC                                                                                                                  <  SRR19973069.1968741/150‑1 (MQ=60)
                                     TATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAAC                                                                                                                  <  SRR19973069.1968703/150‑1 (MQ=60)
                                      ATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACC                                                                                                                 >  SRR19973069.3545985/1‑150 (MQ=60)
                                      ATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACC                                                                                                                 <  SRR19973069.3827208/150‑1 (MQ=60)
                                         GGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGA                                                                                                              >  SRR19973069.23145/1‑150 (MQ=60)
                                         GGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGA                                                                                                              >  SRR19973069.23232/1‑150 (MQ=60)
                                         GGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGA                                                                                                              >  SRR19973069.23231/1‑150 (MQ=60)
                                         GGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGA                                                                                                              >  SRR19973069.23148/1‑150 (MQ=60)
                                         GGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGA                                                                                                              >  SRR19973069.900194/1‑150 (MQ=60)
                                         GGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑AGCCGCAATTTTTTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGA                                                                                                              >  SRR19973069.22977/1‑150 (MQ=60)
                                              CGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAA                                                                                                         >  SRR19973069.1682296/1‑150 (MQ=60)
                                               GTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAAT                                                                                                        >  SRR19973069.2972123/1‑150 (MQ=60)
                                               GTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAAT                                                                                                        >  SRR19973069.2972464/1‑150 (MQ=60)
                                               GTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAAT                                                                                                        <  SRR19973069.776908/150‑1 (MQ=60)
                                                 TCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTTCCGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATA                                                                                                       >  SRR19973069.1465604/1‑150 (MQ=60)
                                                   GCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCG                                                                                                    <  SRR19973069.1729105/150‑1 (MQ=60)
                                                   GCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCG                                                                                                    <  SRR19973069.799281/150‑1 (MQ=60)
                                                    CTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCTCCAGCTCCCAGTCACCGGAATGAATATCGC                                                                                                   >  SRR19973069.2580040/1‑150 (MQ=60)
                                                    CTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGC                                                                                                   >  SRR19973069.1847690/1‑150 (MQ=60)
                                                    CTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGC                                                                                                   >  SRR19973069.1886165/1‑150 (MQ=60)
                                                    CTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGC                                                                                                   >  SRR19973069.3319783/1‑150 (MQ=60)
                                                    CTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGC                                                                                                   >  SRR19973069.3319731/1‑150 (MQ=60)
                                                    CTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGC                                                                                                   >  SRR19973069.1059864/1‑150 (MQ=60)
                                                     TTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCC                                                                                                  >  SRR19973069.2153261/1‑150 (MQ=60)
                                                     TTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCC                                                                                                  >  SRR19973069.2153196/1‑150 (MQ=60)
                                                      TTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCG                                                                                                 >  SRR19973069.3333996/1‑150 (MQ=60)
                                                      TTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCG                                                                                                 >  SRR19973069.3334085/1‑150 (MQ=60)
                                                       TCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGA                                                                                                <  SRR19973069.2377762/150‑1 (MQ=60)
                                                        CAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGAC                                                                                               >  SRR19973069.440004/1‑150 (MQ=60)
                                                        CAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGAC                                                                                               >  SRR19973069.516906/1‑150 (MQ=60)
                                                         AGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACG                                                                                              >  SRR19973069.3018131/1‑150 (MQ=60)
                                                             CGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCG                                                                                          >  SRR19973069.2605057/1‑150 (MQ=60)
                                                              GAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTGGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGG                                                                                         >  SRR19973069.2849479/1‑150 (MQ=60)
                                                              GAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTCACCGGAATGAATATCGCCGACGGGCGG                                                                                         >  SRR19973069.2539609/1‑150 (MQ=60)
                                                              GAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGG                                                                                         >  SRR19973069.2153721/1‑150 (MQ=60)
                                                              GAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGG                                                                                         >  SRR19973069.2539257/1‑150 (MQ=60)
                                                              GAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGG                                                                                         >  SRR19973069.2539303/1‑150 (MQ=60)
                                                                AGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTT                                                                                       >  SRR19973069.2701535/1‑150 (MQ=60)
                                                                 GACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTC                                                                                      <  SRR19973069.3203631/150‑1 (MQ=60)
                                                                           GTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGT                                                                            >  SRR19973069.1497935/1‑150 (MQ=60)
                                                                           GTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGT                                                                            >  SRR19973069.3486695/1‑150 (MQ=60)
                                                                           GTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGT                                                                            >  SRR19973069.3486797/1‑150 (MQ=60)
                                                                           GTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGT                                                                            >  SRR19973069.1497979/1‑150 (MQ=60)
                                                                              CCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACC                                                                         <  SRR19973069.3657472/150‑1 (MQ=60)
                                                                                 GATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCAT                                                                      <  SRR19973069.3468726/150‑1 (MQ=60)
                                                                                  ATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATG                                                                     >  SRR19973069.1887717/1‑150 (MQ=60)
                                                                                       CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCAC                                                                 >  SRR19973069.810810/1‑150 (MQ=60)
                                                                                          CGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTG                                                              >  SRR19973069.3696841/1‑150 (MQ=60)
                                                                                          CGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTG                                                              >  SRR19973069.3696861/1‑150 (MQ=60)
                                                                                          CGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTG                                                              >  SRR19973069.3696862/1‑150 (MQ=60)
                                                                                          CGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCACAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTG                                                              >  SRR19973069.3696844/1‑150 (MQ=60)
                                                                                            CAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTT                                                            >  SRR19973069.3324925/1‑150 (MQ=60)
                                                                                             AATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTT                                                           <  SRR19973069.2892460/150‑1 (MQ=60)
                                                                                               TTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGC                                                         >  SRR19973069.60771/1‑150 (MQ=60)
                                                                                               TTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGC                                                         >  SRR19973069.60398/1‑150 (MQ=60)
                                                                                                         TGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGC                                               <  SRR19973069.25548/150‑1 (MQ=60)
                                                                                                            GCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGT                                            <  SRR19973069.1836250/150‑1 (MQ=60)
                                                                                                            GCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGT                                            <  SRR19973069.3022720/150‑1 (MQ=60)
                                                                                                             CTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTG                                           <  SRR19973069.1537387/150‑1 (MQ=60)
                                                                                                             CTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTG                                           >  SRR19973069.2162591/1‑150 (MQ=60)
                                                                                                             CTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTG                                           >  SRR19973069.2162635/1‑150 (MQ=60)
                                                                                                               AGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCT                                         >  SRR19973069.2221673/1‑150 (MQ=60)
                                                                                                                 CAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCA                                       >  SRR19973069.1959061/1‑150 (MQ=60)
                                                                                                                  AAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCAT                                      >  SRR19973069.942470/1‑150 (MQ=60)
                                                                                                                    AGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCA                                    <  SRR19973069.217651/150‑1 (MQ=60)
                                                                                                                    AGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAAGATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCA                                    <  SRR19973069.3385421/150‑1 (MQ=60)
                                                                                                                     GTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAG                                   <  SRR19973069.738978/150‑1 (MQ=60)
                                                                                                                     GTATTTATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAG                                   <  SRR19973069.738722/150‑1 (MQ=60)
                                                                                                                          TATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAAT                              <  SRR19973069.162825/150‑1 (MQ=60)
                                                                                                                          TATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAAGATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAAT                              <  SRR19973069.198615/150‑1 (MQ=60)
                                                                                                                           ATGCGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATG                             <  SRR19973069.3076841/150‑1 (MQ=60)
                                                                                                                              CGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGG                          <  SRR19973069.3501331/150‑1 (MQ=60)
                                                                                                                              CGCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGG                          >  SRR19973069.2835079/1‑150 (MQ=60)
                                                                                                                               GCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGA                         <  SRR19973069.610126/150‑1 (MQ=60)
                                                                                                                               GCCGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGA                         <  SRR19973069.610105/150‑1 (MQ=60)
                                                                                                                                 CGTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAAC                       >  SRR19973069.3533401/1‑150 (MQ=60)
                                                                                                                                  GTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACC                      <  SRR19973069.1386605/150‑1 (MQ=60)
                                                                                                                                  GTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACC                      <  SRR19973069.1386431/150‑1 (MQ=60)
                                                                                                                                  GTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACC                      <  SRR19973069.1386601/150‑1 (MQ=60)
                                                                                                                                  GTACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAACATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACC                      <  SRR19973069.271941/150‑1 (MQ=60)
                                                                                                                                   TACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCT                     <  SRR19973069.1954969/150‑1 (MQ=60)
                                                                                                                                   TACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCT                     <  SRR19973069.1955079/150‑1 (MQ=60)
                                                                                                                                   TACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAAGATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCT                     <  SRR19973069.1282282/150‑1 (MQ=60)
                                                                                                                                   TACCGTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAACATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCT                     <  SRR19973069.1088518/150‑1 (MQ=60)
                                                                                                                                       GTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCAC                 >  SRR19973069.1902383/1‑150 (MQ=60)
                                                                                                                                       GTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCAC                 >  SRR19973069.1902615/1‑150 (MQ=60)
                                                                                                                                       GTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCAC                 >  SRR19973069.709054/1‑150 (MQ=60)
                                                                                                                                       GTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCAC                 <  SRR19973069.1377305/150‑1 (MQ=60)
                                                                                                                                       GTTAGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATAGCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCAC                 <  SRR19973069.2273270/150‑1 (MQ=60)
                                                                                                                                          AGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGAT              >  SRR19973069.2621171/1‑150 (MQ=60)
                                                                                                                                          AGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGAT              >  SRR19973069.1556525/1‑150 (MQ=60)
                                                                                                                                          AGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGAT              >  SRR19973069.914047/1‑150 (MQ=60)
                                                                                                                                          AGGGCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGAT              >  SRR19973069.914669/1‑150 (MQ=60)
                                                                                                                                             GCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACC           <  SRR19973069.1574760/150‑1 (MQ=60)
                                                                                                                                             GCGGTTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACC           <  SRR19973069.400888/150‑1 (MQ=60)
                                                                                                                                                 TTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATCTCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCA       <  SRR19973069.1247053/150‑1 (MQ=60)
                                                                                                                                                 TTAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCA       <  SRR19973069.1246860/150‑1 (MQ=60)
                                                                                                                                                  TAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCAT      >  SRR19973069.1910884/1‑150 (MQ=60)
                                                                                                                                                  TAGCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCAT      >  SRR19973069.1910690/1‑150 (MQ=60)
                                                                                                                                                    GCTACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTA    <  SRR19973069.304507/150‑1 (MQ=60)
                                                                                                                                                      TACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAAT  >  SRR19973069.2874668/1‑150 (MQ=60)
                                                                                                                                                      |                                                                                                                                                     
TTGTGGTCACGTAAACGCTCGGCAATTTTGTGCGCATTATAGGTTTCGTTCGCTTTCAGACCGAAGACCTGAGCGGTACCGGATTT‑CGCCGCAATTTTGTACGGTGCGCTAGCAAAGTATTTATGCGCCGTACCGTTAGGGCGGTTAGCAACACCGTACATACCGTCTTTCGCCAGCTCCCAGTAACCGGAATGAATATCGCCGACGGGCGGTTCATGCGGCTGTACCCATGGCACCTGTTTGCCGTCTTCGGCGGTGCTCATCAGCAAATGAGGAACCTTCACGATACCGTCATTAAT  >  CP009273/661959‑662257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑