Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 829,009 G→T 85.3% D162Y (GAT→TAT)  dinG → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273829,0090GT85.3% 369.3 / 69.0 169D162Y (GAT→TAT) dinGATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (10/15);  new base T (55/89);  total (65/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 170 total aligned reads displayed.

TTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCG  >  CP009273/828862‑829153
                                                                                                                                                   |                                                                                                                                                
  tgggcgtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTAt                                                                                                                                                <  1:1019116/148‑1 (MQ=255)
  tgggcgtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTAt                                                                                                                                                <  1:1011755/148‑1 (MQ=255)
   gggcgtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTAtc                                                                                                                                               <  1:2726038/148‑1 (MQ=255)
      cgtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCg                                                                                                                                            <  1:2971275/148‑1 (MQ=255)
      cgtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCg                                                                                                                                            <  1:2971253/148‑1 (MQ=255)
      cgtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCg                                                                                                                                            <  1:2970465/148‑1 (MQ=255)
      cgggggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCg                                                                                                                                            <  1:2042825/145‑1 (MQ=255)
         gggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATa                                                                                                                                         >  2:1477584/1‑148 (MQ=255)
         gggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCCAACAAACAGGAAGAGCAAAAACGTTGTGCGAAGCGGAAGGGCGATCTCGATa                                                                                                                                         >  1:2774027/1‑148 (MQ=255)
            cgcTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACtt                                                                                                                                      <  2:800215/148‑1 (MQ=255)
               tACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTtata                                                                                                                                   >  2:1605264/1‑148 (MQ=255)
                 cGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATaaa                                                                                                                                 <  2:2290465/148‑1 (MQ=255)
                   tttGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATg                                                                                                                               <  1:1721074/148‑1 (MQ=255)
                    ttGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATgg                                                                                                                              <  1:1068934/148‑1 (MQ=255)
                     tGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATggg                                                                                                                             <  1:901578/148‑1 (MQ=255)
                     tGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATggg                                                                                                                             <  1:1911783/148‑1 (MQ=255)
                      gTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGa                                                                                                                            <  2:894369/148‑1 (MQ=255)
                      gTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGa                                                                                                                            <  2:2570455/148‑1 (MQ=255)
                       tCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGAt                                                                                                                           >  1:973332/1‑148 (MQ=255)
                             tAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTg                                                                                                                     <  1:1390148/148‑1 (MQ=255)
                             tAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTg                                                                                                                     <  1:1390160/148‑1 (MQ=255)
                              aaTCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTgc                                                                                                                    >  2:325970/1‑148 (MQ=255)
                                      ggCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCAt                                                                                                            <  2:2005828/148‑1 (MQ=255)
                                       gcgcTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATa                                                                                                           <  2:572847/148‑1 (MQ=255)
                                        cgcTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATaa                                                                                                          <  2:2444097/148‑2 (MQ=255)
                                          cTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTg                                                                                                        >  2:108019/1‑148 (MQ=255)
                                           tCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGa                                                                                                       <  2:620796/148‑1 (MQ=255)
                                           tCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGa                                                                                                       <  1:2459398/148‑1 (MQ=255)
                                           tCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGa                                                                                                       <  2:1116610/148‑1 (MQ=255)
                                            cGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGat                                                                                                      <  1:1195218/148‑1 (MQ=255)
                                             gCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGata                                                                                                     >  2:469844/1‑148 (MQ=255)
                                              ccAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGatat                                                                                                    <  2:308840/148‑1 (MQ=255)
                                                aGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCg                                                                                                  >  1:1563639/1‑148 (MQ=255)
                                                 gTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGc                                                                                                 <  1:1145329/148‑1 (MQ=255)
                                                  tACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCt                                                                                                <  2:2923650/148‑1 (MQ=255)
                                                  tACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCt                                                                                                <  2:1730916/148‑1 (MQ=255)
                                                  tACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCt                                                                                                <  2:1962718/148‑1 (MQ=255)
                                                     tGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCtata                                                                                             <  2:723344/148‑1 (MQ=255)
                                                     tGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCtata                                                                                             >  2:3080648/1‑148 (MQ=255)
                                                       aaCCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGa                                                                                           >  1:2713413/1‑148 (MQ=255)
                                                        aCCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGAt                                                                                          <  1:1327821/148‑1 (MQ=255)
                                                         cccACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATg                                                                                         >  2:56747/1‑148 (MQ=255)
                                                          ccACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGa                                                                                        <  2:2506555/148‑1 (MQ=255)
                                                            aCGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACg                                                                                      <  1:1332707/148‑1 (MQ=255)
                                                             cGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGa                                                                                     >  1:2389898/1‑148 (MQ=255)
                                                                  cAGGATCTGCTGGCGTTTATTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATctct                                                                                <  2:1876080/148‑1 (MQ=255)
                                                                       tCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGcgt                                                                           <  1:380681/148‑1 (MQ=255)
                                                                         tgctgGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGcgtcg                                                                         <  1:2623471/148‑1 (MQ=255)
                                                                          gctgGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGcgtcgt                                                                        <  1:927882/148‑1 (MQ=255)
                                                                           ctgGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGtt                                                                       <  2:2487871/148‑1 (MQ=255)
                                                                           ctgGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGtt                                                                       <  1:41778/148‑1 (MQ=255)
                                                                             ggCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTa                                                                     >  2:1709112/1‑148 (MQ=255)
                                                                              gCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTaa                                                                    >  2:2084349/1‑148 (MQ=255)
                                                                              gCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTaa                                                                    <  2:704752/148‑1 (MQ=255)
                                                                              gCGTTACTTGACGACGAACTGACGCCGAACAATCAGGACGAGCAAAAACGTAGTGCGAAGCTCAAGGCAGATCTCGAGACTTATAAATTGGATGTTCTGCGCGATCATACTGATATCGCTAGAGATGAATATCTCTGGCGTCGTTTaa                                                                    >  2:2786819/1‑148 (MQ=255)
                                                                               cGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAg                                                                   >  2:350132/1‑148 (MQ=255)
                                                                                gTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGt                                                                  <  1:32778/148‑1 (MQ=255)
                                                                                      tGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGAc                                                            <  1:3052025/148‑1 (MQ=255)
                                                                                          gacgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAg                                                        <  1:325970/148‑1 (MQ=255)
                                                                                          gacgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAg                                                        <  1:3121463/148‑1 (MQ=255)
                                                                                          gacgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAg                                                        <  1:3010383/148‑1 (MQ=255)
                                                                                          gacgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAg                                                        <  1:1473801/148‑1 (MQ=255)
                                                                                           acgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGc                                                       <  1:2306010/148‑1 (MQ=255)
                                                                                               aCTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGc                                                   <  2:2749072/148‑1 (MQ=255)
                                                                                               aCTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGc                                                   >  2:429492/1‑148 (MQ=255)
                                                                                                  gACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGc                                                >  2:641968/1‑148 (MQ=255)
                                                                                                          aCAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCg                                        >  2:2166722/1‑148 (MQ=255)
                                                                                                          aCAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCg                                        >  2:2862438/1‑148 (MQ=255)
                                                                                                          aCAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCg                                        >  2:1679999/1‑148 (MQ=255)
                                                                                                            aaTCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCa                                      <  1:1605264/148‑1 (MQ=255)
                                                                                                             aTCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCaa                                     >  2:556022/1‑148 (MQ=255)
                                                                                                             aTCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCaa                                     >  2:2539107/1‑148 (MQ=255)
                                                                                                             aTCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCaa                                     <  1:247812/148‑1 (MQ=255)
                                                                                                             aTCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCaa                                     <  1:244320/148‑1 (MQ=255)
                                                                                                                  gAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCCATTGtt                                >  1:2516699/1‑148 (MQ=255)
                                                                                                                  gAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGtt                                >  1:2204998/1‑148 (MQ=255)
                                                                                                                   aaGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTta                               >  2:3135300/1‑148 (MQ=255)
                                                                                                                    agagCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATCCTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTTCCGACAATGCCAGCTGCCTCTCCCGCCCTTGGtac                              >  2:2929878/1‑148 (MQ=255)
                                                                                                                    agagCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCACTTGTtac                              >  2:1281640/1‑148 (MQ=255)
                                                                                                                    agagCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtac                              >  1:1751687/1‑148 (MQ=255)
                                                                                                                    agagCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtac                              >  2:1281650/1‑148 (MQ=255)
                                                                                                                    agagCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTGTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtac                              >  1:1751549/1‑148 (MQ=255)
                                                                                                                     gagCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtact                             >  2:1810066/1‑148 (MQ=255)
                                                                                                                      agCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtacta                            <  2:376700/148‑1 (MQ=255)
                                                                                                                      agCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtacta                            >  1:235856/1‑148 (MQ=255)
                                                                                                                       gCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtactac                           <  1:1927571/148‑1 (MQ=255)
                                                                                                                       gCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtactac                           <  1:1927579/148‑1 (MQ=255)
                                                                                                                         aaaaaCGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTActacta                         <  1:3050697/148‑1 (MQ=255)
                                                                                                                           aaaCGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAATGATACTACTAta                       >  2:116958/1‑147 (MQ=255)
                                                                                                                            aaCGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCg                      <  2:2058632/148‑1 (MQ=255)
                                                                                                                             aCGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGt                     >  1:2268244/1‑148 (MQ=255)
                                                                                                                              cGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTg                    >  1:819878/1‑148 (MQ=255)
                                                                                                                                ttGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGaa                  >  1:396664/1‑148 (MQ=255)
                                                                                                                                    gCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTTGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGcc              <  2:587730/148‑1 (MQ=255)
                                                                                                                                            gAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGtttttt      >  1:756843/1‑148 (MQ=255)
                                                                                                                                             aaGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTg     >  1:1238749/1‑148 (MQ=255)
                                                                                                                                               gggCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTc   >  2:2399311/1‑148 (MQ=255)
                                                                                                                                                ggCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAATTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCg  >  1:1238350/1‑148 (MQ=255)
                                                                                                                                                ggCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCg  >  1:1238396/1‑148 (MQ=255)
                                                                                                                                                ggCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCg  <  1:1914393/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCG  >  CP009273/828862‑829153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGT  >  CP009273/828860‑829158
                                                                                                                                                     |                                                                                                                                                     
CTTTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCT                                                                                                                                                       <  SRR19973070.1214375/150‑1 (MQ=60)
CTTTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCT                                                                                                                                                       <  SRR19973070.1214386/150‑1 (MQ=60)
CTTTTGGGCGTGGGCGCTACGTGTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCT                                                                                                                                                       <  SRR19973070.1213389/150‑1 (MQ=60)
   TTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATC                                                                                                                                                    <  SRR19973070.1389102/150‑1 (MQ=60)
    TGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCT                                                                                                                                                   <  SRR19973070.1026355/150‑1 (MQ=60)
    TGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCT                                                                                                                                                   <  SRR19973070.1018947/150‑1 (MQ=60)
     GGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTC                                                                                                                                                  <  SRR19973070.2743623/150‑1 (MQ=60)
        CGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGAT                                                                                                                                               <  SRR19973070.2990361/150‑1 (MQ=60)
        CGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGAT                                                                                                                                               <  SRR19973070.2989548/150‑1 (MQ=60)
        CGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGAT                                                                                                                                               <  SRR19973070.2990339/150‑1 (MQ=60)
        CGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGAT                                                                                                                                               <  SRR19973070.2990359/150‑1 (MQ=60)
        CGGGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGAT                                                                                                                                               <  SRR19973070.2056644/150‑1 (MQ=60)
         GTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCCAACAAACAGGAAGAGCAAAAACGTTGTGCGAAGCGGAAGGGCGATCTCGATA                                                                                                                                              >  SRR19973070.2791809/1‑150 (MQ=60)
              CGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTAT                                                                                                                                         <  SRR19973070.2351159/150‑1 (MQ=60)
                  ACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAAT                                                                                                                                     <  SRR19973070.595820/150‑1 (MQ=60)
                     TTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGG                                                                                                                                  <  SRR19973070.1732984/150‑1 (MQ=60)
                      TTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGA                                                                                                                                 <  SRR19973070.1076413/150‑1 (MQ=60)
                      TTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGA                                                                                                                                 <  SRR19973070.1076005/150‑1 (MQ=60)
                       TGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGAT                                                                                                                                >  SRR19973070.980275/1‑150 (MQ=60)
                       TGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGAT                                                                                                                                <  SRR19973070.907949/150‑1 (MQ=60)
                       TGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGAT                                                                                                                                <  SRR19973070.907969/150‑1 (MQ=60)
                       TGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGAT                                                                                                                                <  SRR19973070.1924705/150‑1 (MQ=60)
                       TGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGAT                                                                                                                                <  SRR19973070.1924700/150‑1 (MQ=60)
                             CGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTG                                                                                                                          <  SRR19973070.1656231/150‑1 (MQ=60)
                               TAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCG                                                                                                                        <  SRR19973070.1399547/150‑1 (MQ=60)
                               TAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCG                                                                                                                        <  SRR19973070.1399535/150‑1 (MQ=60)
                                      ACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCAT                                                                                                                 <  SRR19973070.11264/150‑1 (MQ=60)
                                           GCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGA                                                                                                            >  SRR19973070.2471160/1‑150 (MQ=60)
                                             TCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATA                                                                                                          <  SRR19973070.2475535/150‑1 (MQ=60)
                                             TCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATA                                                                                                          <  SRR19973070.2475463/150‑1 (MQ=60)
                                              CGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATAT                                                                                                         <  SRR19973070.1203619/150‑1 (MQ=60)
                                              CGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATAT                                                                                                         <  SRR19973070.1203746/150‑1 (MQ=60)
                                                CCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCG                                                                                                       >  SRR19973070.1574645/1‑150 (MQ=60)
                                                   GTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTA                                                                                                    <  SRR19973070.1153287/150‑1 (MQ=60)
                                                       TGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGA                                                                                                >  SRR19973070.2730947/1‑150 (MQ=60)
                                                         cccccaccccacagcatcccctcgcgtctcacGACGACGAACTGACGCCGAACAATCAGCAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATG                                                                                              <  SRR19973070.3305504/118‑1 (MQ=60)
                                                         AACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATG                                                                                              <  SRR19973070.1414855/150‑1 (MQ=60)
                                                          ACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGA                                                                                             <  SRR19973070.1336982/150‑1 (MQ=60)
                                                           CCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATTGGATGGTCTGCGCGATCATACTGATATAGCTATAGATGTC                                                                                            >  SRR19973070.129597/1‑150 (MQ=60)
                                                           CCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATAAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGAC                                                                                            >  SRR19973070.3224999/1‑150 (MQ=60)
                                                             CACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGA                                                                                          >  SRR19973070.2405678/1‑150 (MQ=60)
                                                             CACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAAACAGGAAGAGCAAAAAAGTTGTGCGAAGCTGAAGGGCTATATCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCGATAGATTACGA                                                                                          >  SRR19973070.2405824/1‑150 (MQ=60)
                                                              ACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGAT                                                                                         <  SRR19973070.1341891/150‑1 (MQ=60)
                                                              ACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGAT                                                                                         <  SRR19973070.1341072/150‑1 (MQ=60)
                                                                     AGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGTATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGC                                                                                  >  SRR19973070.2761407/1‑150 (MQ=60)
                                                                         TCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCG                                                                              <  SRR19973070.1394514/150‑1 (MQ=60)
                                                                         TCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCG                                                                              <  SRR19973070.383473/150‑1 (MQ=60)
                                                                           TGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTT                                                                            <  SRR19973070.2640590/150‑1 (MQ=60)
                                                                            GCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTT                                                                           <  SRR19973070.934502/150‑1 (MQ=60)
                                                                             CTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTA                                                                          <  SRR19973070.42309/150‑1 (MQ=60)
                                                                                  GTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTAC                                                                     <  SRR19973070.33204/150‑1 (MQ=60)
                                                                                   TTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACC                                                                    <  SRR19973070.2124428/150‑1 (MQ=60)
                                                                                        TGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAA                                                               <  SRR19973070.3071475/150‑1 (MQ=60)
                                                                                        TGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTGTAAGTACCGACAA                                                               >  SRR19973070.387274/1‑150 (MQ=60)
                                                                                            GACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCC                                                           <  SRR19973070.3029600/150‑1 (MQ=60)
                                                                                            GACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCC                                                           <  SRR19973070.3141610/150‑1 (MQ=60)
                                                                                            GACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCC                                                           <  SRR19973070.328343/150‑1 (MQ=60)
                                                                                            GACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCC                                                           <  SRR19973070.1484109/150‑1 (MQ=60)
                                                                                             ACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCA                                                          <  SRR19973070.2321345/150‑1 (MQ=60)
                                                                                                AACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCT                                                       <  SRR19973070.1487923/150‑1 (MQ=60)
                                                                                                      CGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCA                                                 <  SRR19973070.1741915/150‑1 (MQ=60)
                                                                                                        CCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAAC                                               <  SRR19973070.2540612/150‑1 (MQ=60)
                                                                                                             CAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAA                                          >  SRR19973070.3001413/1‑150 (MQ=60)
                                                                                                              AATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAT                                         <  SRR19973070.1616462/150‑1 (MQ=60)
                                                                                                               ATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATT                                        <  SRR19973070.246001/150‑1 (MQ=60)
                                                                                                               ATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATT                                        <  SRR19973070.249509/150‑1 (MQ=60)
                                                                                                                  AGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCCATTGTT                                     >  SRR19973070.2533096/1‑150 (MQ=60)
                                                                                                                  AGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTT                                     >  SRR19973070.2025153/1‑150 (MQ=60)
                                                                                                                  AGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTT                                     >  SRR19973070.2219521/1‑150 (MQ=60)
                                                                                                                    GAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTAC                                   >  SRR19973070.1763826/1‑150 (MQ=60)
                                                                                                                    GAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCCCAATTGTTAC                                   >  SRR19973070.2809484/1‑150 (MQ=60)
                                                                                                                    GAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTGTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTAC                                   >  SRR19973070.1763688/1‑150 (MQ=60)
                                                                                                                      AGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTA                                 >  SRR19973070.237502/1‑150 (MQ=60)
                                                                                                                       GAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTAC                                <  SRR19973070.961108/150‑1 (MQ=60)
                                                                                                                         GCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTA                              <  SRR19973070.1940568/150‑1 (MQ=60)
                                                                                                                         GCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTA                              <  SRR19973070.1940560/150‑1 (MQ=60)
                                                                                                                           AAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATC                            <  SRR19973070.3070129/150‑1 (MQ=60)
                                                                                                                             AAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATAGT                          >  SRR19973070.2283306/1‑150 (MQ=60)
                                                                                                                             AAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTGTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAATGTTACTACTATCGT                          >  SRR19973070.2283324/1‑150 (MQ=60)
                                                                                                                              AACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTG                         >  SRR19973070.825473/1‑150 (MQ=60)
                                                                                                                                CGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGTA                       >  SRR19973070.399581/1‑150 (MQ=60)
                                                                                                                                 GTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAAT                      <  SRR19973070.3100446/150‑1 (MQ=60)
                                                                                                                                          AGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTCCTATCGTGAATGCCCGTTTT             <  SRR19973070.2659734/150‑1 (MQ=60)
                                                                                                                                            CTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTT           >  SRR19973070.762897/1‑150 (MQ=60)
                                                                                                                                            CTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTT           >  SRR19973070.762179/1‑150 (MQ=60)
                                                                                                                                             TGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATTCCCGTTTTTTG          >  SRR19973070.1247426/1‑150 (MQ=60)
                                                                                                                                                AGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAATTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCG       >  SRR19973070.1247022/1‑150 (MQ=60)
                                                                                                                                                AGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCG       >  SRR19973070.1247068/1‑150 (MQ=60)
                                                                                                                                                AGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCCCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCG       >  SRR19973070.1247067/1‑150 (MQ=60)
                                                                                                                                                  GGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCT     <  SRR19973070.1927321/150‑1 (MQ=60)
                                                                                                                                                  GGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCT     <  SRR19973070.1927364/150‑1 (MQ=60)
                                                                                                                                                  GGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCT     <  SRR19973070.1822545/150‑1 (MQ=60)
                                                                                                                                                  GGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCT     <  SRR19973070.3069157/150‑1 (MQ=60)
                                                                                                                                                    CTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCACTTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCG   >  SRR19973070.1743567/1‑150 (MQ=60)
                                                                                                                                                    CTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAAACCCCATTGTTACTAATATCGTGAATGCCCGTTTTTTTTAGCTCG   >  SRR19973070.306304/1‑150 (MQ=60)
                                                                                                                                                     TATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTTGGGGGGGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGT  >  SRR19973070.1622681/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTTTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGT  >  CP009273/828860‑829158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑