Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 829,009 G→T 100% D162Y (GAT→TAT)  dinG → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273829,0090GT98.0% 628.9 / 3.5 199D162Y (GAT→TAT) dinGATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (82/113);  total (83/116)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 199 total aligned reads displayed.

GGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCT  >  CP009273/828865‑829155
                                                                                                                                                |                                                                                                                                                  
gggcgtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTAtc                                                                                                                                                 <  1:717140/148‑1 (MQ=255)
gggcgtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTATTTACGACGAACAGACGCCGAACAATCAGGAAGAGCAAAAAAGTTGTGCGAAGCAGAAGGGCTAtc                                                                                                                                                 >  1:917864/1‑148 (MQ=255)
   cgtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCg                                                                                                                                              <  1:2126736/148‑1 (MQ=255)
    gtgggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGa                                                                                                                                             <  2:2679107/148‑1 (MQ=255)
      gggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATa                                                                                                                                           >  2:2286518/1‑148 (MQ=255)
      gggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATa                                                                                                                                           <  1:2871987/148‑1 (MQ=255)
       ggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATAc                                                                                                                                          <  2:631919/148‑1 (MQ=255)
       ggcgCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATAc                                                                                                                                          <  2:3310555/148‑1 (MQ=255)
          gcTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTta                                                                                                                                       <  1:3052238/148‑1 (MQ=255)
           cTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTtat                                                                                                                                      >  2:663401/1‑148 (MQ=255)
             aCGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATaa                                                                                                                                    >  2:2879748/1‑148 (MQ=255)
              cGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATaaa                                                                                                                                   <  2:3137269/148‑1 (MQ=255)
              cGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATaaa                                                                                                                                   >  2:456892/1‑148 (MQ=255)
               gTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAAt                                                                                                                                  <  2:3024970/148‑1 (MQ=255)
               gTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAAt                                                                                                                                  <  2:3262684/148‑1 (MQ=255)
                tttGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATg                                                                                                                                 >  1:1749659/1‑148 (MQ=255)
                 ttGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATgg                                                                                                                                >  2:1458550/1‑148 (MQ=255)
                  tGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATggg                                                                                                                               <  1:1381098/148‑1 (MQ=255)
                     ccGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATg                                                                                                                            <  2:1975339/148‑1 (MQ=255)
                     ccGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATg                                                                                                                            >  2:518902/1‑148 (MQ=255)
                     ccGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATg                                                                                                                            <  2:1270430/148‑1 (MQ=255)
                          tAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTg                                                                                                                       <  1:1340648/148‑1 (MQ=255)
                            aTCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTgcg                                                                                                                     <  2:2653897/148‑1 (MQ=255)
                               tGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGa                                                                                                                  <  1:1312637/148‑1 (MQ=255)
                                gACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGAt                                                                                                                 <  1:3481245/148‑1 (MQ=255)
                                   ggCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCAt                                                                                                              <  1:1904610/148‑1 (MQ=255)
                                   ggCGCTCGCCAGTACAGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCAt                                                                                                              <  2:1656689/148‑1 (MQ=255)
                                     cgcTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATAc                                                                                                            >  1:162562/1‑148 (MQ=255)
                                        tCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGa                                                                                                         <  2:3178142/148‑1 (MQ=255)
                                          gCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGata                                                                                                       <  1:1675850/148‑1 (MQ=255)
                                              gTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGc                                                                                                   <  1:1687171/148‑1 (MQ=255)
                                              gTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGc                                                                                                   <  1:2514995/148‑1 (MQ=255)
                                               tACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAAAGTTGTGCGAAGCTGAAGGGCTATCTCGATACGTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCt                                                                                                  >  1:451312/1‑148 (MQ=255)
                                               tACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAAAGTTGTGCGAAGCGGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCt                                                                                                  >  1:3323205/1‑148 (MQ=255)
                                               tACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACAGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCt                                                                                                  >  1:1962090/1‑148 (MQ=255)
                                                   gAACCCACGCAACAGGATCTGCTGGCGTGTATTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATTGGATGGTCTGCGCGATCAAACTGATATCGCTATAg                                                                                              >  2:2379326/1‑148 (MQ=255)
                                                    aaCCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGa                                                                                             <  2:2050135/148‑1 (MQ=255)
                                                     aCCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGAt                                                                                            >  1:813020/1‑148 (MQ=255)
                                                      cccACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATg                                                                                           <  1:2800400/148‑1 (MQ=255)
                                                      cccACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATg                                                                                           <  1:1653749/148‑1 (MQ=255)
                                                       ccACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGa                                                                                          <  1:3380053/148‑1 (MQ=255)
                                                       ccACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGa                                                                                          <  1:1444446/148‑1 (MQ=255)
                                                        cACGCAACAGGATCTGCTGGCGTTTCTTGACGACTAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGAc                                                                                         <  2:10493/148‑1 (MQ=255)
                                                         aCGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACg                                                                                        <  1:3146205/148‑1 (MQ=255)
                                                          cGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGa                                                                                       >  2:1128801/1‑148 (MQ=255)
                                                              aCAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGAtctc                                                                                   <  1:2097500/148‑1 (MQ=255)
                                                               cAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATctct                                                                                  >  1:1448654/1‑148 (MQ=255)
                                                                 ggATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTgg                                                                                <  1:3259327/148‑1 (MQ=255)
                                                                  gATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCGATAGATGACGATCTCTGGc                                                                               >  2:1026946/1‑148 (MQ=255)
                                                                   aTCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGcg                                                                              >  2:2126885/1‑148 (MQ=255)
                                                                   aTCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGcg                                                                              >  2:3435386/1‑148 (MQ=255)
                                                                    tCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGcgt                                                                             <  1:149650/148‑1 (MQ=255)
                                                                         tgGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATACATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGttt                                                                        <  1:2410847/148‑1 (MQ=255)
                                                                         tgGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGttt                                                                        <  2:234348/148‑1 (MQ=255)
                                                                          ggCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTa                                                                       <  2:1654502/148‑1 (MQ=255)
                                                                          ggCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTa                                                                       <  2:977227/148‑1 (MQ=255)
                                                                           gCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTaa                                                                      >  2:2401527/1‑148 (MQ=255)
                                                                            cGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAg                                                                     <  1:826367/148‑1 (MQ=255)
                                                                              tttCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTa                                                                   <  2:2635260/148‑1 (MQ=255)
                                                                               ttCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTAc                                                                  >  2:2213965/1‑148 (MQ=255)
                                                                               ttCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTAc                                                                  >  1:1370275/1‑148 (MQ=255)
                                                                               ttCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTAc                                                                  >  1:1465597/1‑148 (MQ=255)
                                                                                tCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTAcc                                                                 >  2:3501536/1‑148 (MQ=255)
                                                                                  ttGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGa                                                               <  1:2725271/148‑1 (MQ=255)
                                                                                     acgacgAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACaa                                                            <  1:103870/148‑1 (MQ=255)
                                                                                     acgacgAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACaa                                                            <  2:2688854/148‑1 (MQ=255)
                                                                                        acgaACTGACGCCGAACAGTCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGc                                                         >  2:2198213/1‑148 (MQ=255)
                                                                                        acgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGc                                                         >  1:2304600/1‑148 (MQ=255)
                                                                                        acgaACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGc                                                         <  2:847283/148‑1 (MQ=255)
                                                                                            aCTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGc                                                     >  2:1149418/1‑148 (MQ=255)
                                                                                                aCGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGcc                                                 <  1:3106924/148‑1 (MQ=255)
                                                                                                       aCAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCg                                          >  1:3030915/1‑148 (MQ=255)
                                                                                                           tCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAt                                      >  2:1530755/1‑148 (MQ=255)
                                                                                                            cAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAtt                                     <  1:1209742/148‑1 (MQ=255)
                                                                                                            cAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCGGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAtt                                     <  2:2409428/148‑1 (MQ=255)
                                                                                                             aGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCCGCTGCCTCAACCGCCATTg                                    >  2:2013146/1‑148 (MQ=255)
                                                                                                             aGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTg                                    <  2:1352156/148‑1 (MQ=255)
                                                                                                               gAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCCATTGtt                                  >  2:3037264/1‑148 (MQ=255)
                                                                                                                aaGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTta                                 <  1:708147/148‑1 (MQ=255)
                                                                                                                 agagCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtac                                >  2:2666568/1‑148 (MQ=255)
                                                                                                                 agagCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTtac                                <  1:2668278/148‑1 (MQ=255)
                                                                                                                  gagCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAGTGTtact                               >  1:2867599/1‑148 (MQ=255)
                                                                                                                     cAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTactact                            >  1:2740661/1‑148 (MQ=255)
                                                                                                                     cAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTactact                            <  1:476121/148‑1 (MQ=255)
                                                                                                                         aaCGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCg                        >  2:2096964/1‑148 (MQ=255)
                                                                                                                          aCGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGt                       <  1:3501536/148‑1 (MQ=255)
                                                                                                                          aCGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGt                       >  1:498940/1‑148 (MQ=255)
                                                                                                                           cGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTg                      >  1:214534/1‑148 (MQ=255)
                                                                                                                                    aaGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGt             <  1:2038197/148‑1 (MQ=255)
                                                                                                                                     aGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATTCCCGtt            >  2:1717501/1‑148 (MQ=255)
                                                                                                                                      gCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGttt           >  2:260417/1‑148 (MQ=255)
                                                                                                                                       cTGCAGGGCTATCTCGATACTGATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGtttt          <  2:3512403/148‑1 (MQ=255)
                                                                                                                                       cTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGtttt          <  2:1558695/148‑1 (MQ=255)
                                                                                                                                       cTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGtttt          <  2:2649425/148‑1 (MQ=255)
                                                                                                                                       cTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTGTAAGTAACGACAAAGCCAGCTGCCTCAACCCCAATTGTTACTACTATCGTGAATGCCCGtttt          >  1:1556881/1‑148 (MQ=255)
                                                                                                                                          aaGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTg       <  2:2027307/148‑1 (MQ=255)
                                                                                                                                          aaGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTAATATCGTGAATGCCCGTTTTTTg       >  2:908027/1‑148 (MQ=255)
                                                                                                                                           aGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGt      >  1:1158848/1‑148 (MQ=255)
                                                                                                                                            gggCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAATGTTACTACTATCGTGAATGCCCGTTTTTTGTc     >  2:3078983/1‑148 (MQ=255)
                                                                                                                                               cGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCt  >  1:2318979/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCT  >  CP009273/828865‑829155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGT  >  CP009273/828860‑829158
                                                                                                                                                     |                                                                                                                                                     
CTTTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCT                                                                                                                                                       <  SRR19973065.1774393/150‑1 (MQ=60)
CTTTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCT                                                                                                                                                       >  SRR19973065.1361105/1‑150 (MQ=60)
CTTTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCT                                                                                                                                                       >  SRR19973065.217967/1‑150 (MQ=60)
CTTTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCT                                                                                                                                                       <  SRR19973065.2303835/150‑1 (MQ=60)
 TTTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTA                                                                                                                                                      <  SRR19973065.335014/150‑1 (MQ=60)
 TTTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTA                                                                                                                                                      <  SRR19973065.335055/150‑1 (MQ=60)
  TTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTAT                                                                                                                                                     <  SRR19973065.1639007/150‑1 (MQ=60)
   TTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATC                                                                                                                                                    <  SRR19973065.2754058/150‑1 (MQ=60)
   TTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTATTTACGACGAACAGACGCCGAACAATCAGGAAGAGCAAAAAAGTTGTGCGAAGCAGAAGGGCTATC                                                                                                                                                    >  SRR19973065.923870/1‑150 (MQ=60)
     GGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTC                                                                                                                                                  <  SRR19973065.721940/150‑1 (MQ=60)
     GGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTC                                                                                                                                                  <  SRR19973065.719298/150‑1 (MQ=60)
        CGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGAT                                                                                                                                               <  SRR19973065.2140422/150‑1 (MQ=60)
           GGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACT                                                                                                                                            <  SRR19973065.2889508/150‑1 (MQ=60)
           GGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACT                                                                                                                                            <  SRR19973065.2889588/150‑1 (MQ=60)
               GCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATA                                                                                                                                        <  SRR19973065.3070900/150‑1 (MQ=60)
                 TACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAAATGACGCCGAACAATCAGGAAGAGCAAAAAAGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAA                                                                                                                                      >  SRR19973065.1329974/1‑150 (MQ=60)
                   CGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATG                                                                                                                                    >  SRR19973065.1761185/1‑150 (MQ=60)
                      TTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGA                                                                                                                                 <  SRR19973065.3088092/150‑1 (MQ=60)
                      TTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGA                                                                                                                                 <  SRR19973065.1592973/150‑1 (MQ=60)
                       TGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGAT                                                                                                                                <  SRR19973065.1390191/150‑1 (MQ=60)
                       TGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGAT                                                                                                                                <  SRR19973065.1675234/150‑1 (MQ=60)
                          CCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGT                                                                                                                             <  SRR19973065.2058695/150‑1 (MQ=60)
                           CGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTC                                                                                                                            >  SRR19973065.2000322/1‑150 (MQ=60)
                           CGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGAACTGCTGGCGTTTCATTAAGACGAACTGCCGCCGAACAAACACGAACAGCAAAAACCTTGTGCCAAGCTGAAGGGCAATCACGAAACTTATAAAAGGGAGGGTC                                                                                                                            >  SRR19973065.1624024/1‑150 (MQ=60)
                               TAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCG                                                                                                                        <  SRR19973065.1928142/150‑1 (MQ=60)
                               TAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCG                                                                                                                        <  SRR19973065.1349520/150‑1 (MQ=60)
                               TAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCG                                                                                                                        <  SRR19973065.1349532/150‑1 (MQ=60)
                                    TGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATC                                                                                                                   <  SRR19973065.1321313/150‑1 (MQ=60)
                                     GACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCT                                                                                                                  <  SRR19973065.3502587/150‑1 (MQ=60)
                                     GACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCA                                                                                                                  <  SRR19973065.3502429/150‑1 (MQ=60)
                                       CGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATA                                                                                                                <  SRR19973065.2832099/150‑1 (MQ=60)
                                        GGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATAC                                                                                                               <  SRR19973065.2982483/150‑1 (MQ=60)
                                        GGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATAC                                                                                                               >  SRR19973065.163894/1‑150 (MQ=60)
                                        GGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATAC                                                                                                               <  SRR19973065.2629644/150‑1 (MQ=60)
                                        GGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATAC                                                                                                               <  SRR19973065.1917124/150‑1 (MQ=60)
                                               GCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATC                                                                                                        <  SRR19973065.1687063/150‑1 (MQ=60)
                                                CCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCG                                                                                                       <  SRR19973065.2319777/150‑1 (MQ=60)
                                                  AGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACGTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCT                                                                                                     >  SRR19973065.3343739/1‑150 (MQ=60)
                                                  AGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATATCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCT                                                                                                     >  SRR19973065.496131/1‑150 (MQ=60)
                                                  AGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAAAGTTGTGCGAAGCTGAAGGGCTATCTCGATACGTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCT                                                                                                     >  SRR19973065.454439/1‑150 (MQ=60)
                                                  AGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAAAGTTGTGCGAAGCGGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCT                                                                                                     >  SRR19973065.3343483/1‑150 (MQ=60)
                                                  AGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACAGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCT                                                                                                     >  SRR19973065.1974903/1‑150 (MQ=60)
                                                  AGTACTGAACCCACGCAACAGGATCTGCTGGCGTGTGGTTACGACGAACTGAACCCGAAAAATCAGGAAGATCAAAAAAGTTGTGCGCAGCTGAACGGCGATCTCGATACGTATAAATGGGATGGTCTGCGCGATCAgacagtaatgggt                                                                                                     >  SRR19973065.3343800/1‑137 (MQ=60)
                                                   GTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTA                                                                                                    <  SRR19973065.2530797/150‑1 (MQ=60)
                                                   GTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTA                                                                                                    <  SRR19973065.1698443/150‑1 (MQ=60)
                                                       TGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGTTGGTCTGCGCGATCATACTGATATCGCTATAGA                                                                                                >  SRR19973065.1386389/1‑150 (MQ=60)
                                                        GAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGAT                                                                                               >  SRR19973065.818382/1‑150 (MQ=60)
                                                        GAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGAT                                                                                               <  SRR19973065.2780056/150‑1 (MQ=60)
                                                           CCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGAC                                                                                            <  SRR19973065.1664837/150‑1 (MQ=60)
                                                           CCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGAC                                                                                            <  SRR19973065.3413294/150‑1 (MQ=60)
                                                           CCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGAC                                                                                            <  SRR19973065.1664830/150‑1 (MQ=60)
                                                           CCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGAC                                                                                            <  SRR19973065.753582/150‑1 (MQ=60)
                                                           CCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGAC                                                                                            <  SRR19973065.2817744/150‑1 (MQ=60)
                                                            CCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACG                                                                                           <  SRR19973065.1453788/150‑1 (MQ=60)
                                                            CCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACG                                                                                           <  SRR19973065.3400718/150‑1 (MQ=60)
                                                              ACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGAT                                                                                         <  SRR19973065.3165313/150‑1 (MQ=60)
                                                                  AACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCT                                                                                     >  SRR19973065.1458011/1‑150 (MQ=60)
                                                                   ACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTG                                                                                    <  SRR19973065.2110992/150‑1 (MQ=60)
                                                                   ACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTG                                                                                    <  SRR19973065.2111097/150‑1 (MQ=60)
                                                                     AGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGC                                                                                  >  SRR19973065.1698259/1‑150 (MQ=60)
                                                                      GGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCG                                                                                 <  SRR19973065.3279058/150‑1 (MQ=60)
                                                                         TCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCG                                                                              <  SRR19973065.150908/150‑1 (MQ=60)
                                                                           TGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTT                                                                            <  SRR19973065.667801/150‑1 (MQ=60)
                                                                              TGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATACATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAA                                                                         <  SRR19973065.2426025/150‑1 (MQ=60)
                                                                              TGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAA                                                                         <  SRR19973065.2425263/150‑1 (MQ=60)
                                                                              TGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAA                                                                         >  SRR19973065.1489601/1‑150 (MQ=60)
                                                                                 CGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTA                                                                      <  SRR19973065.831779/150‑1 (MQ=60)
                                                                                  GTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTAC                                                                     >  SRR19973065.1475002/1‑150 (MQ=60)
                                                                                  GTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTAC                                                                     >  SRR19973065.1379326/1‑150 (MQ=60)
                                                                                  GTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTAC                                                                     <  SRR19973065.445407/150‑1 (MQ=60)
                                                                                   TTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAACTGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACC                                                                    <  SRR19973065.3264384/150‑1 (MQ=60)
                                                                                   TTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACC                                                                    <  SRR19973065.3063586/150‑1 (MQ=60)
                                                                                     TCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGA                                                                  <  SRR19973065.854329/150‑1 (MQ=60)
                                                                                       TTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACA                                                                <  SRR19973065.2742222/150‑1 (MQ=60)
                                                                                       TTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACA                                                                >  SRR19973065.3290287/1‑150 (MQ=60)
                                                                                          ACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAG                                                             <  SRR19973065.104824/150‑1 (MQ=60)
                                                                                           CGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGC                                                            >  SRR19973065.2319088/1‑150 (MQ=60)
                                                                                            GACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCC                                                           <  SRR19973065.1269600/150‑1 (MQ=60)
                                                                                               GAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACACAGCCAGC                                                        >  SRR19973065.456197/1‑150 (MQ=60)
                                                                                                 ACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTG                                                      >  SRR19973065.2467124/1‑150 (MQ=60)
                                                                                                    GACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCT                                                   >  SRR19973065.2756618/1‑150 (MQ=60)
                                                                                                     ACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTC                                                  <  SRR19973065.3125878/150‑1 (MQ=60)
                                                                                                     ACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTC                                                  <  SRR19973065.3126563/150‑1 (MQ=60)
                                                                                                        CCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTGTAAGTACCGACAAAGCCAGCTGCCTCAAC                                               >  SRR19973065.1706706/1‑150 (MQ=60)
                                                                                                         CGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCCGCTGCCTCAACC                                              >  SRR19973065.2141858/1‑150 (MQ=60)
                                                                                                          GAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCG                                             >  SRR19973065.100876/1‑150 (MQ=60)
                                                                                                          GAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCG                                             >  SRR19973065.3049482/1‑150 (MQ=60)
                                                                                                              AATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAT                                         >  SRR19973065.271041/1‑150 (MQ=60)
                                                                                                                TCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTG                                       <  SRR19973065.1454827/150‑1 (MQ=60)
                                                                                                                 CAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGT                                      <  SRR19973065.1217544/150‑1 (MQ=60)
                                                                                                                  AGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTT                                     <  SRR19973065.176733/150‑1 (MQ=60)
                                                                                                                     AAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACT                                  <  SRR19973065.712873/150‑1 (MQ=60)
                                                                                                                     AAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAAGTGTTACT                                  >  SRR19973065.2885190/1‑150 (MQ=60)
                                                                                                                      AGAGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTA                                 <  SRR19973065.2684801/150‑1 (MQ=60)
                                                                                                                        AGCAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCACCTGCCTCAACCGCAATTGTTACTACT                               >  SRR19973065.2757707/1‑150 (MQ=60)
                                                                                                                          CAAAAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTAT                             <  SRR19973065.479367/150‑1 (MQ=60)
                                                                                                                             AAACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGT                          >  SRR19973065.502287/1‑150 (MQ=60)
                                                                                                                              AACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTG                         >  SRR19973065.216152/1‑150 (MQ=60)
                                                                                                                               ACGTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGA                        <  SRR19973065.3522800/150‑1 (MQ=60)
                                                                                                                                 GTTGTGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTATGTCGTCGTTTAAataacgaaaaaaccacaatcctcaaacgccaattttactaatatagtgaat                      >  SRR19973065.1541299/1‑99 (MQ=60)
                                                                                                                                     TGCGAAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCC                  <  SRR19973065.2300931/150‑1 (MQ=60)
                                                                                                                                         AAGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTT              <  SRR19973065.2051257/150‑1 (MQ=60)
                                                                                                                                          AGCTGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTGTAAGTAACGACAAAGCCAGCTGCCTCAACCCCAATTGTTACTACTATCGTGAATGCCCGTTTT             >  SRR19973065.1567213/1‑150 (MQ=60)
                                                                                                                                             TGAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTGTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTG          >  SRR19973065.825712/1‑150 (MQ=60)
                                                                                                                                              GAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGT         >  SRR19973065.1167093/1‑150 (MQ=60)
                                                                                                                                              GAAGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGT         >  SRR19973065.1166275/1‑150 (MQ=60)
                                                                                                                                                AGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCG       <  SRR19973065.1467932/150‑1 (MQ=60)
                                                                                                                                                AGGGCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCG       <  SRR19973065.1437373/150‑1 (MQ=60)
                                                                                                                                                  GGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCT     >  SRR19973065.2333512/1‑150 (MQ=60)
                                                                                                                                                   GCTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTC    >  SRR19973065.1008416/1‑150 (MQ=60)
                                                                                                                                                    CTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCG   <  SRR19973065.942656/150‑1 (MQ=60)
                                                                                                                                                    CTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCG   >  SRR19973065.1030738/1‑150 (MQ=60)
                                                                                                                                                    CTATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCG   <  SRR19973065.184717/150‑1 (MQ=60)
                                                                                                                                                     TATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGT  >  SRR19973065.1751968/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTTTTGGGCGTGGGCGCTACGTTTGTCCGCGTAATCTGACGGCGCTCGCCAGTACTGAACCCACGCAACAGGATCTGCTGGCGTTTCTTGACGACGAACTGACGCCGAACAATCAGGAAGAGCAAAAACGTTGTGCGAAGCTGAAGGGCGATCTCGATACTTATAAATGGGATGGTCTGCGCGATCATACTGATATCGCTATAGATGACGATCTCTGGCGTCGTTTAAGTACCGACAAAGCCAGCTGCCTCAACCGCAATTGTTACTACTATCGTGAATGCCCGTTTTTTGTCGCTCGT  >  CP009273/828860‑829158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑