Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 877,482 C→A 6.0% D237Y (GAT→TAT)  deoR ← deoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273877,4820CA 6.0% 624.4 / 12.6 165D237Y (GAT→TAT) deoRdeoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx
Reads supporting (aligned to +/- strand):  ref base C (84/71);  new base A (7/3);  total (91/74)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 169 total aligned reads displayed.

GATTTCGTGATGATGAAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCAACTCTTC  >  CP009273/877335‑877629
                                                                                                                                                   |                                                                                                                                                   
gATTTCGTGATGATGAAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATc                                                                                                                                                     <  1:920270/148‑1 (MQ=255)
   ttCGTGATGATGAAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTc                                                                                                                                                  <  2:742474/148‑1 (MQ=255)
   ttCGTGATGATGAAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTc                                                                                                                                                  <  2:2400949/148‑1 (MQ=255)
    tCGTGATGATGAAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTcc                                                                                                                                                 <  2:1065994/148‑1 (MQ=255)
    tCGTGATGATGAAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTcc                                                                                                                                                 >  2:186144/1‑148 (MQ=255)
       tgatgatgaAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCggg                                                                                                                                              <  2:488499/148‑1 (MQ=255)
        gatgatgaAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGc                                                                                                                                             >  2:2925694/1‑148 (MQ=255)
         atgatgaAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGca                                                                                                                                            >  1:3189864/1‑148 (MQ=255)
             tgaAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGcaacaa                                                                                                                                        <  2:2993568/148‑1 (MQ=255)
              gaAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAAt                                                                                                                                       <  1:1143365/148‑1 (MQ=255)
              gaAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAAt                                                                                                                                       <  1:2795461/148‑1 (MQ=255)
              gaAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAAt                                                                                                                                       >  2:3182763/1‑148 (MQ=255)
                 aTTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGc                                                                                                                                    >  2:540696/1‑148 (MQ=255)
                 aTTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGc                                                                                                                                    <  1:1397381/148‑1 (MQ=255)
                    ccATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTca                                                                                                                                 <  2:1775327/148‑1 (MQ=255)
                      aTCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTcacc                                                                                                                               >  2:1440029/1‑148 (MQ=255)
                           tGGTTGGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAat                                                                                                                          <  2:3082568/148‑1 (MQ=255)
                           tGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAat                                                                                                                          <  2:3082797/148‑1 (MQ=255)
                                cGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCaa                                                                                                                     <  1:1307416/148‑1 (MQ=255)
                                  gCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAg                                                                                                                   <  2:1629912/148‑1 (MQ=255)
                                      cTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGtt                                                                                                               >  2:2460764/1‑148 (MQ=255)
                                        ggTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTc                                                                                                             >  2:683446/1‑148 (MQ=255)
                                          ttCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAg                                                                                                           >  2:818133/1‑148 (MQ=255)
                                          ttCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAg                                                                                                           >  2:818238/1‑148 (MQ=255)
                                            ctctTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGt                                                                                                         <  2:327088/148‑1 (MQ=255)
                                               ttAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCAc                                                                                                      >  2:798110/1‑148 (MQ=255)
                                               ttAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCAc                                                                                                      >  2:58993/1‑148 (MQ=255)
                                                tAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCAcc                                                                                                     >  1:1181828/1‑148 (MQ=255)
                                                tAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCAcc                                                                                                     <  1:3239580/148‑1 (MQ=255)
                                                 aaTCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCAccc                                                                                                    <  2:2645846/148‑1 (MQ=255)
                                                  aTCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCa                                                                                                   >  2:1917224/1‑148 (MQ=255)
                                                          aTAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGcc                                                                                           >  1:2528277/1‑148 (MQ=255)
                                                          aTAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGcc                                                                                           >  2:1873371/1‑148 (MQ=255)
                                                             aCCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGa                                                                                        >  1:2395451/1‑148 (MQ=255)
                                                              ccGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGAc                                                                                       >  1:2467825/1‑148 (MQ=255)
                                                                 gATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCa                                                                                    >  1:2338129/1‑148 (MQ=255)
                                                                  aTGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTCCTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCACAGCGTTTCAGGTCACCCATGCGCGCCGGACGCAc                                                                                   >  1:1806334/1‑148 (MQ=255)
                                                                  aTGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCAc                                                                                   >  1:1806353/1‑148 (MQ=255)
                                                                     gcgcGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCtt                                                                                >  1:512750/1‑148 (MQ=255)
                                                                     gcgcGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCtt                                                                                >  2:3166545/1‑148 (MQ=255)
                                                                            aCGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAAt                                                                         >  2:3138887/1‑148 (MQ=255)
                                                                             cGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAAtt                                                                        >  1:1887621/1‑148 (MQ=255)
                                                                             cGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAAtt                                                                        >  2:3302647/1‑148 (MQ=255)
                                                                                cATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTAc                                                                     >  2:1497752/1‑148 (MQ=255)
                                                                                cATCCGGTTATACGTCATTAATAAATCAACTTAATGCGCTGCGACTGCGCGTACTTCACATACTCATCTTCAGGGCAACAATCTATCACCACAATATCAAAGCGTTTCAGCTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTaa                                                                     >  1:893829/1‑147 (MQ=255)
                                                                                  tCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACtg                                                                   <  2:3310267/148‑1 (MQ=255)
                                                                                  tCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACAGACTCATCGTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACtg                                                                   <  2:1475896/148‑1 (MQ=255)
                                                                                   ccGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACtgt                                                                  >  1:3133047/1‑148 (MQ=255)
                                                                                    cGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACtgtg                                                                 >  1:3246764/1‑148 (MQ=255)
                                                                                     ggTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTgg                                                                <  2:3056736/148‑1 (MQ=255)
                                                                                      gTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGt                                                               <  1:1440029/148‑1 (MQ=255)
                                                                                      gTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGt                                                               <  2:1249072/148‑1 (MQ=255)
                                                                                       ttATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTc                                                              >  1:2597599/1‑148 (MQ=255)
                                                                                        tataCGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCg                                                             <  2:2875708/148‑1 (MQ=255)
                                                                                              tCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAAcc                                                       >  2:340895/1‑148 (MQ=255)
                                                                                                aTTAATACATCAACTTAATGCGCGGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAg                                                     <  2:2729230/148‑1 (MQ=255)
                                                                                                 ttAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGc                                                    >  1:2236422/1‑148 (MQ=255)
                                                                                                  tAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGca                                                   >  1:2893965/1‑148 (MQ=255)
                                                                                                  tAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGca                                                   >  1:3185284/1‑148 (MQ=255)
                                                                                                      aCATCAACTTACTGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCCAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATg                                               <  2:2096290/148‑1 (MQ=255)
                                                                                                      aCATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATg                                               <  1:3298299/148‑1 (MQ=255)
                                                                                                       cATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGc                                              <  1:2069778/148‑1 (MQ=255)
                                                                                                       cATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGc                                              <  1:2725629/148‑1 (MQ=255)
                                                                                                        aTCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCt                                             <  1:383841/148‑1 (MQ=255)
                                                                                                        aTCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCt                                             <  1:383833/148‑1 (MQ=255)
                                                                                                         tCAGCTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCtt                                            >  2:2468696/1‑148 (MQ=255)
                                                                                                         tCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCtt                                            >  2:1216995/1‑148 (MQ=255)
                                                                                                         tCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCtt                                            >  2:2750905/1‑148 (MQ=255)
                                                                                                          cAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCttt                                           >  2:73120/1‑148 (MQ=255)
                                                                                                           aaCTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCtttt                                          <  2:419472/148‑1 (MQ=255)
                                                                                                            aCTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTg                                         >  2:2543734/1‑148 (MQ=255)
                                                                                                                 aTGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCa                                    >  2:386530/1‑148 (MQ=255)
                                                                                                                 aTGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCa                                    >  1:1772499/1‑148 (MQ=255)
                                                                                                                  tGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCAt                                   >  1:474921/1‑148 (MQ=255)
                                                                                                                   gcgcTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATc                                  <  2:831123/148‑1 (MQ=255)
                                                                                                                      cTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGAc                               <  1:2750905/148‑1 (MQ=255)
                                                                                                                      cTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGAc                               >  1:107110/1‑148 (MQ=255)
                                                                                                                        gCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACAt                             >  2:892153/1‑148 (MQ=255)
                                                                                                                        gCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACAt                             <  1:2441649/148‑1 (MQ=255)
                                                                                                                            cTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGcc                         >  1:3031564/1‑148 (MQ=255)
                                                                                                                                 cgTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAgtg                    <  2:2108433/148‑1 (MQ=255)
                                                                                                                                  gTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAgtgt                   <  1:827027/148‑1 (MQ=255)
                                                                                                                                    aCTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGttt                 >  1:613564/1‑148 (MQ=255)
                                                                                                                                     cTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGtttt                <  1:2719067/148‑1 (MQ=255)
                                                                                                                                     cTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGtttt                >  1:500101/1‑148 (MQ=255)
                                                                                                                                     cTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGtttt                <  1:340827/148‑1 (MQ=255)
                                                                                                                                     cTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGGGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGtttt                >  1:500236/1‑148 (MQ=255)
                                                                                                                                      ttCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTa               <  1:3182763/148‑1 (MQ=255)
                                                                                                                                       tCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTAc              >  2:421217/1‑148 (MQ=255)
                                                                                                                                          caTACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCgg           >  2:1696448/1‑148 (MQ=255)
                                                                                                                                           aTACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCCTCGACATGGCCCAGGGTTTTACCGGc          >  2:1968711/1‑148 (MQ=255)
                                                                                                                                            tACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCa         >  1:929056/1‑148 (MQ=255)
                                                                                                                                            tACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCa         >  1:2678898/1‑148 (MQ=255)
                                                                                                                                            tACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCa         >  1:2678890/1‑148 (MQ=255)
                                                                                                                                            tACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCa         >  1:1025091/1‑148 (MQ=255)
                                                                                                                                             aCTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCaa        >  2:1368955/1‑148 (MQ=255)
                                                                                                                                                cATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCAActc     <  1:798110/148‑1 (MQ=255)
                                                                                                                                                cATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCAActc     >  1:104697/1‑148 (MQ=255)
                                                                                                                                                   cTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCAACTCTTc  <  1:2045065/148‑1 (MQ=255)
                                                                                                                                                   cTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCAACTCTTc  <  1:2045053/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GATTTCGTGATGATGAAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCAACTCTTC  >  CP009273/877335‑877629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A