Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 877,482 C→A 28.8% D237Y (GAT→TAT)  deoR ← deoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273877,4820CA28.8% 492.8 / 187.9 239D237Y (GAT→TAT) deoRdeoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx
Reads supporting (aligned to +/- strand):  ref base C (77/93);  new base A (38/31);  total (115/124)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 239 total aligned reads displayed.

GGATTTCGTGATGATGAAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCAACTCTTC  >  CP009273/877334‑877629
                                                                                                                                                    |                                                                                                                                                   
ggATTTCGTGATGATGAAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCt                                                                                                                                                    >  1:1025872/1‑150 (MQ=255)
ggATTTCGTGATGATGAAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCt                                                                                                                                                    <  2:3140891/150‑1 (MQ=255)
  aTTTCGTGATGATGAAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTc                                                                                                                                                  <  2:2081918/150‑1 (MQ=255)
         gatgatgaAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGcaa                                                                                                                                           >  1:3706234/1‑150 (MQ=255)
          atgatgaAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGcaac                                                                                                                                          <  2:4610594/150‑1 (MQ=255)
           tgatgaAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGcaaca                                                                                                                                         >  2:2934886/1‑150 (MQ=255)
           tgatgaAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGcaaca                                                                                                                                         <  2:1238644/150‑1 (MQ=255)
             atgaAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAAt                                                                                                                                       <  1:4014362/150‑1 (MQ=255)
              tgaAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATc                                                                                                                                      <  1:1514782/150‑1 (MQ=255)
               gaAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCg                                                                                                                                     <  2:210330/150‑1 (MQ=255)
                 aaTTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCt                                                                                                                                   >  1:4447694/1‑150 (MQ=255)
                   ttCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTca                                                                                                                                 <  1:2415418/150‑1 (MQ=255)
                     ccATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTcacc                                                                                                                               <  1:1385369/150‑1 (MQ=255)
                     ccATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTcacc                                                                                                                               <  2:2277708/150‑1 (MQ=255)
                      cATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTcacca                                                                                                                              >  1:3501536/1‑150 (MQ=255)
                        tCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACcaca                                                                                                                            <  2:814053/150‑1 (MQ=255)
                           gTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAata                                                                                                                         >  1:346174/1‑150 (MQ=255)
                              gTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCa                                                                                                                      <  1:2036125/150‑1 (MQ=255)
                                 cGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAg                                                                                                                   >  1:4283612/1‑150 (MQ=255)
                                     aGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGtt                                                                                                               <  2:3547471/150‑1 (MQ=255)
                                       cTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTc                                                                                                             >  1:2302794/1‑150 (MQ=255)
                                        tGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCa                                                                                                            <  1:476004/150‑1 (MQ=255)
                                          gTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAgg                                                                                                          <  1:3790899/150‑1 (MQ=255)
                                           ttCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGt                                                                                                         >  1:2093630/1‑150 (MQ=255)
                                           ttCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGt                                                                                                         >  2:1961365/1‑150 (MQ=255)
                                             ctctTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCa                                                                                                       >  1:1317556/1‑150 (MQ=255)
                                               ctTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCAcc                                                                                                     <  2:3901287/150‑1 (MQ=255)
                                                ttAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCAccc                                                                                                    <  1:609417/150‑1 (MQ=255)
                                                 tAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCa                                                                                                   >  2:946000/1‑150 (MQ=255)
                                                 tAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCa                                                                                                   <  2:740912/150‑1 (MQ=255)
                                                 tAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCa                                                                                                   >  2:4524181/1‑150 (MQ=255)
                                                  aaTCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCAt                                                                                                  >  2:400452/1‑150 (MQ=255)
                                                  aaTCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCAt                                                                                                  >  1:2121909/1‑150 (MQ=255)
                                                  aaTCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCAt                                                                                                  <  2:1687042/150‑1 (MQ=255)
                                                   aTCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATg                                                                                                 <  2:785334/150‑1 (MQ=255)
                                                    tcttctGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATgc                                                                                                <  2:1443593/150‑1 (MQ=255)
                                                    tcttctGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATgc                                                                                                <  2:3853564/150‑1 (MQ=255)
                                                    tcttctGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATgc                                                                                                >  1:4298229/1‑150 (MQ=255)
                                                     cttctGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGTTCACCCATgcg                                                                                               >  2:1982723/1‑150 (MQ=255)
                                                       tctGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATgcgcg                                                                                             <  1:659756/150‑1 (MQ=255)
                                                        ctGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATgcgcgc                                                                                            >  1:4719163/1‑150 (MQ=255)
                                                           aTAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCgg                                                                                         >  2:1789707/1‑150 (MQ=255)
                                                           aTAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCgg                                                                                         >  2:4196121/1‑150 (MQ=255)
                                                           aTAACCGGATGGCGCGAAACGTCATCCGGTTATACGACATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCAGGCGCGCCgg                                                                                         >  1:1782880/1‑150 (MQ=255)
                                                            tAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGa                                                                                        >  2:2848088/1‑150 (MQ=255)
                                                              aCCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACg                                                                                      >  2:4747643/1‑150 (MQ=255)
                                                                 ggATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCAc                                                                                   >  2:4126950/1‑150 (MQ=255)
                                                                  gATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCAcc                                                                                  <  2:4648884/150‑1 (MQ=255)
                                                                    tGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCAGCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCtt                                                                                <  2:4305356/150‑1 (MQ=255)
                                                                      gcgcGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGc                                                                              <  2:582670/150‑1 (MQ=255)
                                                                       cgcgAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGcc                                                                             <  2:36707/150‑1 (MQ=255)
                                                                         cgAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCaa                                                                           >  2:3914566/1‑150 (MQ=255)
                                                                         cgAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCaa                                                                           >  2:2761752/1‑150 (MQ=255)
                                                                         cgAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCaa                                                                           >  2:4125155/1‑150 (MQ=255)
                                                                         cgAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGATGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCAACACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCAACTTGCCaa                                                                           >  2:2713776/1‑150 (MQ=255)
                                                                           aaaCGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAAt                                                                         >  2:3626563/1‑150 (MQ=255)
                                                                           aaaCGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAAt                                                                         <  1:1893546/150‑1 (MQ=255)
                                                                               gTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTAc                                                                     <  1:3532213/150‑1 (MQ=255)
                                                                                tCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACt                                                                    <  1:1639931/150‑1 (MQ=255)
                                                                                tCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACt                                                                    >  2:2585798/1‑150 (MQ=255)
                                                                                  aTCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACtgt                                                                  <  1:3287777/150‑1 (MQ=255)
                                                                                   tCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACtgtg                                                                 >  1:2936688/1‑150 (MQ=255)
                                                                                      ggTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTc                                                              <  1:2448995/150‑1 (MQ=255)
                                                                                        ttATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGa                                                            >  1:3061998/1‑150 (MQ=255)
                                                                                           taCGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACaa                                                         >  1:4585396/1‑150 (MQ=255)
                                                                                           taCGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACaa                                                         >  1:3909579/1‑150 (MQ=255)
                                                                                            aCGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAAc                                                        <  2:4655412/150‑1 (MQ=255)
                                                                                             cGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAAcc                                                       >  1:1634547/1‑150 (MQ=255)
                                                                                              gTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCa                                                      >  2:2442400/1‑150 (MQ=255)
                                                                                              gTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCa                                                      >  2:3449957/1‑150 (MQ=255)
                                                                                                cATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGc                                                    <  1:1172063/150‑1 (MQ=255)
                                                                                                   tAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGcaca                                                 >  2:2264481/1‑150 (MQ=255)
                                                                                                    aaTACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACAt                                                >  1:3617349/1‑150 (MQ=255)
                                                                                                     aTACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATg                                               >  1:3188227/1‑150 (MQ=255)
                                                                                                       aCATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCt                                             <  1:3870741/150‑1 (MQ=255)
                                                                                                         aTCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCttt                                           >  2:1705641/1‑150 (MQ=255)
                                                                                                          tCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCtttt                                          <  1:1093938/150‑1 (MQ=255)
                                                                                                                tAATGCGCTGCGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCa                                    >  1:3253732/1‑150 (MQ=255)
                                                                                                                   tGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCg                                 <  1:1801602/150‑1 (MQ=255)
                                                                                                                          cGTCTGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGc                          >  2:732055/1‑150 (MQ=255)
                                                                                                                              tGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAg                      >  1:4397633/1‑150 (MQ=255)
                                                                                                                              tGCGCGTACTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAg                      <  2:3689522/150‑1 (MQ=255)
                                                                                                                                 gcgTACTTCACATACTCCTCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAgtgt                   <  1:813024/150‑1 (MQ=255)
                                                                                                                                 gcgTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAgtgt                   <  1:4043096/150‑1 (MQ=255)
                                                                                                                                 gcgTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAgtgt                   <  2:4423432/150‑1 (MQ=255)
                                                                                                                                 gcgTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAgtgt                   <  2:2019421/150‑1 (MQ=255)
                                                                                                                                  cgTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGtt                  <  2:4091772/150‑1 (MQ=255)
                                                                                                                                  cgTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGtt                  <  2:333774/150‑1 (MQ=255)
                                                                                                                                  cgTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGtt                  <  1:3626668/150‑1 (MQ=255)
                                                                                                                                   gTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGttt                 >  2:363445/1‑150 (MQ=255)
                                                                                                                                     aCTTCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTa               <  2:1317556/150‑1 (MQ=255)
                                                                                                                                        tCACATACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCg            <  2:1090027/150‑1 (MQ=255)
                                                                                                                                         cacaTACTCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCgg           >  1:450121/1‑150 (MQ=255)
                                                                                                                                             tACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCAAc       <  1:2729333/150‑1 (MQ=255)
                                                                                                                                              aCTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCAAct      <  2:1268139/150‑1 (MQ=255)
                                                                                                                                                tCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGTAActct    <  1:3876735/150‑1 (MQ=255)
                                                                                                                                                tCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCAActct    >  2:1520209/1‑150 (MQ=255)
                                                                                                                                                tCATATTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCAActct    <  1:4203130/150‑1 (MQ=255)
                                                                                                                                                 cATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCAACTCtt   <  2:3188227/150‑1 (MQ=255)
                                                                                                                                                 cATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCAACTCtt   <  1:3233356/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GGATTTCGTGATGATGAAATTCCATCAGTGGTTCGGCAGCTGGTTCTCTTAATCTTCTGATAACCGGATGGCGCGAAACGTCATCCGGTTATACGTCATTAATACATCAACTTAATGCGCTGCGTCTGCGCGTACTTCACATACTCATCTTCCGGGCAACAATCGCTCACCACAATATCAAAGCGTTTCAGGTCACCCATGCGCGCCGGACGCACCTTGCCAAATTTACTGTGGTCGACAACCAGCACATGCTTTTGCGCCATCGACATGGCCCAGTGTTTTACCGGCAACTCTTC  >  CP009273/877334‑877629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A