Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 907,596 G→T 8.1% P4Q (CCA→CAA)  hcr ← HCP oxidoreductase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273907,5960GT 8.1% 479.4 / 15.7 135P4Q (CCA→CAA) hcrHCP oxidoreductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (58/66);  new base T (6/5);  total (64/71)
Fisher's exact test for biased strand distribution p-value = 7.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01

Only 100 of 137 total aligned reads displayed.

GTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCGGTGACGA  >  CP009273/907449‑907742
                                                                                                                                                   |                                                                                                                                                  
gTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTg                                                                                                                                                    <  2:803234/148‑1 (MQ=255)
  ttCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGc                                                                                                                                                  >  2:830341/1‑148 (MQ=255)
  ttCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGc                                                                                                                                                  >  2:3134621/1‑148 (MQ=255)
                 cACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACaaa                                                                                                                                   >  2:2195860/1‑148 (MQ=255)
                   cGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACt                                                                                                                                 >  1:951469/1‑148 (MQ=255)
                    gCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTc                                                                                                                                <  1:60330/148‑1 (MQ=255)
                     cTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTcc                                                                                                                               >  2:783813/1‑148 (MQ=255)
                     cTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTcc                                                                                                                               >  2:2118203/1‑148 (MQ=255)
                      tGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCt                                                                                                                              <  2:2507637/148‑1 (MQ=255)
                       gACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGCGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCtt                                                                                                                             <  1:1778124/148‑1 (MQ=255)
                        aCCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTa                                                                                                                            >  2:2017589/1‑148 (MQ=255)
                         ccAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTAc                                                                                                                           <  2:1628695/148‑1 (MQ=255)
                            gTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATTAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTAcgcg                                                                                                                        >  1:567570/1‑148 (MQ=255)
                             tGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACgcgc                                                                                                                       <  1:914836/148‑1 (MQ=255)
                              gCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCt                                                                                                                      <  2:1441772/148‑1 (MQ=255)
                                  atTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAAc                                                                                                                  >  2:1433945/1‑148 (MQ=255)
                                  atTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAAc                                                                                                                  >  1:1785262/1‑148 (MQ=255)
                                   ttGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACa                                                                                                                 >  1:3305505/1‑148 (MQ=255)
                                        cGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGc                                                                                                            <  1:1597332/148‑1 (MQ=255)
                                        cGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCAACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGc                                                                                                            >  2:1071878/1‑148 (MQ=255)
                                        cGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCAACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGc                                                                                                            >  2:2199144/1‑148 (MQ=255)
                                           cgcgATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTc                                                                                                         <  1:2550813/148‑1 (MQ=255)
                                           cgcgATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTc                                                                                                         <  1:2551083/148‑1 (MQ=255)
                                             cgATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCAt                                                                                                       >  1:2111116/1‑148 (MQ=255)
                                             cgATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCAt                                                                                                       >  1:1308781/1‑148 (MQ=255)
                                             cgATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCAt                                                                                                       >  1:1308796/1‑148 (MQ=255)
                                               atatGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGt                                                                                                     <  2:211871/148‑1 (MQ=255)
                                               atatGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGt                                                                                                     <  1:337648/148‑1 (MQ=255)
                                                tatGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGtc                                                                                                    >  2:2008732/1‑148 (MQ=255)
                                                tatGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGtc                                                                                                    >  1:923571/1‑148 (MQ=255)
                                                tatGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGtc                                                                                                    <  2:897036/148‑1 (MQ=255)
                                                 atGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGtct                                                                                                   >  1:2284249/1‑148 (MQ=255)
                                                  tGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGtctt                                                                                                  <  1:2464945/148‑1 (MQ=255)
                                                  tGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGtctt                                                                                                  <  1:3195539/148‑1 (MQ=255)
                                                  tGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGtctt                                                                                                  <  2:3039536/148‑1 (MQ=255)
                                                    ggTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGtcttct                                                                                                >  2:595937/1‑148 (MQ=255)
                                                      tagtaATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCttcttc                                                                                              <  1:1887630/148‑1 (MQ=255)
                                                            tCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAgt                                                                                        >  2:716660/1‑148 (MQ=255)
                                                            tCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAgt                                                                                        >  2:2872847/1‑148 (MQ=255)
                                                             cGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAgtg                                                                                       >  2:1255105/1‑148 (MQ=255)
                                                              gTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAgtgg                                                                                      <  1:2765772/148‑1 (MQ=255)
                                                              gTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAgtgg                                                                                      <  1:501671/148‑1 (MQ=255)
                                                               tGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAgtggt                                                                                     >  1:630915/1‑148 (MQ=255)
                                                                  cAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGAt                                                                                  <  2:2080440/148‑1 (MQ=255)
                                                                     aTCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAAAAGTGGTGATAGa                                                                               <  2:2568621/148‑1 (MQ=255)
                                                                       cAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAAc                                                                             >  2:2957173/1‑148 (MQ=255)
                                                                            aaaTCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAgg                                                                        <  1:1571682/148‑1 (MQ=255)
                                                                             aaTCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGc                                                                       >  2:2873326/1‑148 (MQ=255)
                                                                             aaTCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGc                                                                       >  2:1224716/1‑148 (MQ=255)
                                                                              aTCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGcc                                                                      >  2:3129547/1‑148 (MQ=255)
                                                                              aTCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGcc                                                                      >  1:2274897/1‑148 (MQ=255)
                                                                               tCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCg                                                                     >  2:3103105/1‑148 (MQ=255)
                                                                                cGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGa                                                                    <  1:925507/148‑1 (MQ=255)
                                                                                   ccACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAAtt                                                                 >  2:2372543/1‑148 (MQ=255)
                                                                                      cacaTCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTtct                                                              <  1:3257743/148‑1 (MQ=255)
                                                                                      cacaTCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTtct                                                              <  1:3257807/148‑1 (MQ=255)
                                                                                      cacaTCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAAATCCTTACGCGCTCAACACTTGCTTCATGTCTTATTCAACAGTGGTGATAGAAAGCAGGCCGAAtttat                                                              >  1:59490/1‑146 (MQ=255)
                                                                                              gCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGc                                                      <  1:1344861/148‑1 (MQ=255)
                                                                                              gCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGc                                                      <  1:2621919/148‑1 (MQ=255)
                                                                                               cGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCa                                                     <  1:1457746/148‑1 (MQ=255)
                                                                                                gTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCAc                                                    >  2:3310721/1‑148 (MQ=255)
                                                                                                  ttCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCg                                                  >  2:869077/1‑148 (MQ=255)
                                                                                                  ttCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCg                                                  >  1:3083434/1‑148 (MQ=255)
                                                                                                  ttCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCg                                                  >  2:2678170/1‑148 (MQ=255)
                                                                                                  ttCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCg                                                  >  2:3274435/1‑148 (MQ=255)
                                                                                                  ttCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCg                                                  <  1:2275553/148‑1 (MQ=255)
                                                                                                   tCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGc                                                 <  2:1769800/148‑1 (MQ=255)
                                                                                                    cTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGcc                                                <  2:416803/148‑1 (MQ=255)
                                                                                                    cTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGcc                                                <  2:2336890/148‑1 (MQ=255)
                                                                                                     ttGCGTAATGTGATGAACCTGAATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCca                                               >  2:838906/1‑148 (MQ=255)
                                                                                                      tGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCcag                                              <  2:702555/148‑1 (MQ=255)
                                                                                                      tGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCcag                                              <  2:703102/148‑1 (MQ=255)
                                                                                                       gCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCcagc                                             <  2:1191074/148‑1 (MQ=255)
                                                                                                        cGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCcagca                                            <  2:1709167/148‑1 (MQ=255)
                                                                                                           aaTGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGt                                         <  2:827969/148‑1 (MQ=255)
                                                                                                                gatgaACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGgt                                    <  1:3255196/148‑1 (MQ=255)
                                                                                                                 atgaACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGgtg                                   <  1:3134621/148‑1 (MQ=255)
                                                                                                                  tgaACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGgtgt                                  <  2:1651861/148‑1 (MQ=255)
                                                                                                                  tgaACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGgtgt                                  <  1:563550/148‑1 (MQ=255)
                                                                                                                     aCCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAg                               >  2:1203586/1‑148 (MQ=255)
                                                                                                                        tGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGaa                            >  1:2982390/1‑148 (MQ=255)
                                                                                                                        tGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGaa                            >  1:231022/1‑148 (MQ=255)
                                                                                                                        tGCATCCGCCACGGGAATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGaa                            <  2:1622097/148‑1 (MQ=255)
                                                                                                                         gCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTGTTCTTCAACAGTGGTGATAGAACGCAGGCCGAAAATCTCGTTCAGCACCGCCACAAGGTCTGGTGTCAGGaaa                           >  2:3277476/1‑148 (MQ=255)
                                                                                                                         gCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGaaa                           <  2:671810/148‑1 (MQ=255)
                                                                                                                          cATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAAc                          >  1:995220/1‑148 (MQ=255)
                                                                                                                          cATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAAc                          <  2:65716/148‑1 (MQ=255)
                                                                                                                            tCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCa                        <  1:783813/148‑1 (MQ=255)
                                                                                                                             ccGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAg                       >  2:3037934/1‑148 (MQ=255)
                                                                                                                             ccGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAg                       <  1:1441202/148‑1 (MQ=255)
                                                                                                                                   cGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAg                 <  1:3092137/148‑1 (MQ=255)
                                                                                                                                        aTTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGACCGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCggg            >  2:1615157/1‑148 (MQ=255)
                                                                                                                                        aTTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCggg            >  2:2424612/1‑148 (MQ=255)
                                                                                                                                         ttGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGc           >  1:2166284/1‑148 (MQ=255)
                                                                                                                                           gATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCg         <  1:3256716/148‑1 (MQ=255)
                                                                                                                                           gATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCg         <  1:3256737/148‑1 (MQ=255)
                                                                                                                                            aTTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCgg        >  1:1602286/1‑148 (MQ=255)
                                                                                                                                              tCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCGGTg      >  1:1429083/1‑148 (MQ=255)
                                                                                                                                                 ttGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCGGTGACg   >  2:56238/1‑148 (MQ=255)
                                                                                                                                                  tGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCGGTGACGa  <  2:2225987/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCGGTGACGA  >  CP009273/907449‑907742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A