Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 907,596 G→T 35.5% P4Q (CCA→CAA)  hcr ← HCP oxidoreductase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273907,5960GT35.5% 477.0 / 311.0 309P4Q (CCA→CAA) hcrHCP oxidoreductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (103/96);  new base T (57/53);  total (160/149)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 311 total aligned reads displayed.

GCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCGGTGACGAT  >  CP009273/907447‑907743
                                                                                                                                                     |                                                                                                                                                   
gCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTg                                                                                                                                                     <  2:1766772/150‑1 (MQ=255)
     tCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATc                                                                                                                                                <  1:1247074/150‑1 (MQ=255)
     tCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATc                                                                                                                                                <  2:4763973/150‑1 (MQ=255)
       cGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGt                                                                                                                                              >  2:663926/1‑150 (MQ=255)
        gCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTc                                                                                                                                             >  2:4232805/1‑150 (MQ=255)
         cTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCa                                                                                                                                            >  2:3482008/1‑150 (MQ=255)
            aGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCg                                                                                                                                         >  2:4075933/1‑150 (MQ=255)
             gTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGa                                                                                                                                        >  1:5071180/1‑150 (MQ=255)
              tttCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGAc                                                                                                                                       >  2:544827/1‑150 (MQ=255)
               ttCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACa                                                                                                                                      <  1:1632088/150‑1 (MQ=255)
               ttCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACa                                                                                                                                      <  2:1167454/150‑1 (MQ=255)
                tCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACaa                                                                                                                                     <  1:4130214/150‑1 (MQ=255)
                tCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACaa                                                                                                                                     >  2:791731/1‑150 (MQ=255)
                 cGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACaaa                                                                                                                                    <  1:2511287/150‑1 (MQ=255)
                 cGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACaaa                                                                                                                                    >  2:3538981/1‑150 (MQ=255)
                    aCGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTc                                                                                                                                 >  1:5120771/1‑150 (MQ=255)
                    aCGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTc                                                                                                                                 >  1:3365081/1‑150 (MQ=255)
                      gCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCt                                                                                                                               <  2:225068/150‑1 (MQ=255)
                        tGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTa                                                                                                                             >  1:3080656/1‑150 (MQ=255)
                         gACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTAc                                                                                                                            <  2:2592585/150‑1 (MQ=255)
                         gACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTAc                                                                                                                            >  1:2827525/1‑150 (MQ=255)
                         gACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTAc                                                                                                                            <  1:1696294/150‑1 (MQ=255)
                          aCCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTAcg                                                                                                                           <  1:47671/150‑1 (MQ=255)
                             aGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACgcgc                                                                                                                        >  1:3869840/1‑150 (MQ=255)
                             aGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACgcgc                                                                                                                        >  2:2910798/1‑150 (MQ=255)
                                    atTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAg                                                                                                                 >  2:3715823/1‑150 (MQ=255)
                                     ttGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGt                                                                                                                <  1:841545/150‑1 (MQ=255)
                                      tGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGtt                                                                                                               >  1:680748/1‑150 (MQ=255)
                                      tGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGtt                                                                                                               <  1:1005713/150‑1 (MQ=255)
                                       gCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTg                                                                                                              >  1:1907/1‑150 (MQ=255)
                                       gCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTg                                                                                                              >  1:3713807/1‑150 (MQ=255)
                                           ggCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTc                                                                                                          <  2:2377973/150‑1 (MQ=255)
                                           ggCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTc                                                                                                          <  1:4592710/150‑1 (MQ=255)
                                           ggCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTc                                                                                                          <  2:3847823/150‑1 (MQ=255)
                                             cgcgATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCAt                                                                                                        >  1:269115/1‑150 (MQ=255)
                                             cgcgATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCAt                                                                                                        >  2:4948056/1‑150 (MQ=255)
                                                        tagtaATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCaa                                                                                             >  2:3118684/1‑150 (MQ=255)
                                                        tagtaATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCGTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCaa                                                                                             <  2:1476905/150‑1 (MQ=255)
                                                         agtaATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAAc                                                                                            >  1:2748656/1‑150 (MQ=255)
                                                          gtaATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACa                                                                                           >  1:2935192/1‑150 (MQ=255)
                                                             aTCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAgtg                                                                                        <  2:1968543/150‑1 (MQ=255)
                                                             aTCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAgtg                                                                                        <  1:4458775/150‑1 (MQ=255)
                                                             aTCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAgtg                                                                                        >  1:5045004/1‑150 (MQ=255)
                                                              tCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAgtgg                                                                                       <  1:153697/150‑1 (MQ=255)
                                                              tCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAgtgg                                                                                       <  2:4346814/150‑1 (MQ=255)
                                                                   gCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATa                                                                                  >  2:5146237/1‑150 (MQ=255)
                                                                   gCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTATTCTTCAAAAGTGGTGATa                                                                                  >  2:2603888/1‑150 (MQ=255)
                                                                    cAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAg                                                                                 >  2:1508920/1‑150 (MQ=255)
                                                                      aaTCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGaa                                                                               >  2:1146300/1‑150 (MQ=255)
                                                                       aTCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAAc                                                                              <  2:457893/150‑1 (MQ=255)
                                                                       aTCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAAc                                                                              <  2:4816042/150‑1 (MQ=255)
                                                                       aTCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAAc                                                                              <  1:4073018/150‑1 (MQ=255)
                                                                        tCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACg                                                                             <  2:5067542/150‑1 (MQ=255)
                                                                         cAGGGAAATCGTCCTCACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCAGTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGc                                                                            >  2:1886422/1‑150 (MQ=255)
                                                                         cAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGc                                                                            >  1:4277301/1‑150 (MQ=255)
                                                                         cAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGc                                                                            >  1:3480760/1‑150 (MQ=255)
                                                                         cAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGc                                                                            >  2:4122081/1‑150 (MQ=255)
                                                                          aGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCa                                                                           >  1:2789440/1‑150 (MQ=255)
                                                                          aGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCa                                                                           >  2:3727588/1‑150 (MQ=255)
                                                                             gAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGc                                                                        >  2:4644262/1‑150 (MQ=255)
                                                                             gAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGc                                                                        >  1:2142165/1‑150 (MQ=255)
                                                                               aaTCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCg                                                                      <  1:1073612/150‑1 (MQ=255)
                                                                                   gTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAAtt                                                                  >  2:3365893/1‑150 (MQ=255)
                                                                                   gTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAAtt                                                                  >  1:3636438/1‑150 (MQ=255)
                                                                                   gTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACTCAGGCCGAAtt                                                                  >  1:1640064/1‑150 (MQ=255)
                                                                                   gTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAAtt                                                                  >  1:4684732/1‑150 (MQ=255)
                                                                                         acaTCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGt                                                            >  2:3592051/1‑150 (MQ=255)
                                                                                          caTCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGtt                                                           >  2:1320384/1‑150 (MQ=255)
                                                                                            tCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCa                                                         <  1:2985488/150‑1 (MQ=255)
                                                                                               ggCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCa                                                      <  1:3315917/150‑1 (MQ=255)
                                                                                                      cTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCcag                                               >  2:900319/1‑150 (MQ=255)
                                                                                                      cTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCcag                                               <  2:1419655/150‑1 (MQ=255)
                                                                                                      cTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCcag                                               >  1:3503600/1‑150 (MQ=255)
                                                                                                      cTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCcag                                               >  1:4942085/1‑150 (MQ=255)
                                                                                                       ttGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCcagc                                              >  1:2823762/1‑150 (MQ=255)
                                                                                                           gTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGt                                          <  1:1465666/150‑1 (MQ=255)
                                                                                                            tAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTc                                         >  2:3086506/1‑150 (MQ=255)
                                                                                                             aaTGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCt                                        >  1:1729330/1‑150 (MQ=255)
                                                                                                                  gatgaACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGgtgt                                   >  2:565115/1‑150 (MQ=255)
                                                                                                                     gaACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAg                                >  2:4730114/1‑150 (MQ=255)
                                                                                                                     gaACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAg                                <  2:4884730/150‑1 (MQ=255)
                                                                                                                       aCCTGCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGCTCTGGTGTCAGGa                              >  2:2052520/1‑150 (MQ=255)
                                                                                                                           gCATCCGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAAcc                          >  1:4964777/1‑150 (MQ=255)
                                                                                                                            cATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCa                         <  2:109463/150‑1 (MQ=255)
                                                                                                                            cATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCa                         >  2:2927154/1‑150 (MQ=255)
                                                                                                                               ccGCCACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGt                      >  2:3290315/1‑150 (MQ=255)
                                                                                                                                  ccACGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCa                   <  2:273652/150‑1 (MQ=255)
                                                                                                                                    aCGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGAAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGt                 <  1:765356/150‑1 (MQ=255)
                                                                                                                                    aCGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGt                 >  1:1398810/1‑150 (MQ=255)
                                                                                                                                     cGGGCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTc                <  2:3597727/150‑1 (MQ=255)
                                                                                                                                     cGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTc                <  1:490116/150‑1 (MQ=255)
                                                                                                                                       ggCATTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCgg              >  1:4216812/1‑150 (MQ=255)
                                                                                                                                         cATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGc            <  1:984252/150‑1 (MQ=255)
                                                                                                                                          aTTGATTCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGcc           >  2:949702/1‑150 (MQ=255)
                                                                                                                                          aTTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGcc           <  2:180048/150‑1 (MQ=255)
                                                                                                                                           ttGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCg          <  2:1706171/150‑1 (MQ=255)
                                                                                                                                             gATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCGGt        >  2:4559296/1‑150 (MQ=255)
                                                                                                                                                tCGTTTGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCGGTGAc     <  1:4015533/150‑1 (MQ=255)
                                                                                                                                                 cGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCGGTGACg    >  1:1863018/1‑150 (MQ=255)
                                                                                                                                                 cGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCGGTGACg    <  1:3958739/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAGCAGGTCTGGTGTCAGGAAACCAGGTGCAGTCGGGCCGGTGACGAT  >  CP009273/907447‑907743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A