Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,113,450 A→T 91.6% I161I (ATT→ATA)  yceI ← secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,113,4500AT91.6% 499.4 / 31.0 167I161I (ATT→ATA) yceIsecreted protein
Reads supporting (aligned to +/- strand):  ref base A (6/8);  new base T (98/55);  total (104/63)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 167 total aligned reads displayed.

CAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTC  >  CP009273/1113305‑1113594
                                                                                                                                                 |                                                                                                                                                
  aCAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTAttt                                                                                                                                              <  2:2897542/148‑1 (MQ=255)
   cAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTAtttt                                                                                                                                             >  2:3262943/1‑148 (MQ=255)
   cAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTAtttt                                                                                                                                             >  1:517622/1‑148 (MQ=255)
    aGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCCTCCACTGNAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTg                                                                                                                                            <  1:1683358/148‑1 (MQ=255)
     gAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGc                                                                                                                                           <  2:2476358/148‑1 (MQ=255)
     gAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGc                                                                                                                                           <  2:2476344/148‑1 (MQ=255)
      aataaataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGcc                                                                                                                                          >  2:706056/1‑148 (MQ=255)
      aataaataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGcc                                                                                                                                          >  1:1727676/1‑148 (MQ=255)
      aataaataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCAGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTGTTGATATTGAATTCCTTGAGCTTTATTTTGcc                                                                                                                                          >  1:2724341/1‑148 (MQ=255)
       ataaataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCt                                                                                                                                         <  2:2542792/148‑1 (MQ=255)
        taaataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCtt                                                                                                                                        <  2:2227481/148‑1 (MQ=255)
        taaataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGACtt                                                                                                                                        >  2:1747207/1‑148 (MQ=255)
           atatCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCgg                                                                                                                                     >  2:2057823/1‑148 (MQ=255)
            tatCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGc                                                                                                                                    <  2:1022145/148‑1 (MQ=255)
            tatCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGc                                                                                                                                    >  1:3199411/1‑148 (MQ=255)
            tatCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGc                                                                                                                                    >  1:768489/1‑148 (MQ=255)
                 ttGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGa                                                                                                                               >  1:2957964/1‑148 (MQ=255)
                  tGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGaa                                                                                                                              >  2:103867/1‑148 (MQ=255)
                       gATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGACGCCTg                                                                                                                         >  2:1700811/1‑148 (MQ=255)
                             tGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGtt                                                                                                                   >  2:2236283/1‑148 (MQ=255)
                             tGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGtt                                                                                                                   >  2:729473/1‑148 (MQ=255)
                             tGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGtt                                                                                                                   >  2:920407/1‑148 (MQ=255)
                             tGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGtt                                                                                                                   >  2:2605382/1‑148 (MQ=255)
                               ccATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTa                                                                                                                 <  2:953743/148‑1 (MQ=255)
                               ccATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTa                                                                                                                 >  1:2047672/1‑148 (MQ=255)
                                 aTCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTacc                                                                                                               <  1:2152126/148‑1 (MQ=255)
                                  tCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTacca                                                                                                              >  1:1763058/1‑148 (MQ=255)
                                  tCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTacca                                                                                                              >  1:3027318/1‑148 (MQ=255)
                                   ccGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTaccac                                                                                                             >  2:3297195/1‑148 (MQ=255)
                                      gCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCAcccc                                                                                                          >  1:2638097/1‑148 (MQ=255)
                                       cAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCa                                                                                                         >  2:1792571/1‑148 (MQ=255)
                                       cAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCa                                                                                                         >  2:2716529/1‑148 (MQ=255)
                                            tttCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGgt                                                                                                    <  1:993993/148‑1 (MQ=255)
                                              tCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGgtcg                                                                                                  <  2:3105833/148‑1 (MQ=255)
                                                   cTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGcc                                                                                             <  2:394560/148‑1 (MQ=255)
                                                    ttACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGccc                                                                                            >  2:644883/1‑148 (MQ=255)
                                                    ttACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGccc                                                                                            <  1:2607645/148‑1 (MQ=255)
                                                    ttACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGccc                                                                                            <  1:1969907/148‑1 (MQ=255)
                                                        ttCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGAc                                                                                        >  2:2121020/1‑148 (MQ=255)
                                                        ttCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGAc                                                                                        >  2:1218244/1‑148 (MQ=255)
                                                          ctgctgTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCa                                                                                      <  1:2865658/148‑1 (MQ=255)
                                                           tgctgTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACTTTTACCACCCCATGGGTCGTCGCCCTGACCaa                                                                                     <  2:857294/148‑1 (MQ=255)
                                                               gTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCaattaa                                                                                 <  1:2365128/148‑1 (MQ=255)
                                                               gTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCaattaa                                                                                 >  1:1589323/1‑148 (MQ=255)
                                                                 aCGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCaattaatt                                                                               >  1:2659402/1‑148 (MQ=255)
                                                                 aCGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCaattaatt                                                                               <  2:144011/148‑1 (MQ=255)
                                                                  cGCCGTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAAttt                                                                              <  1:3262943/148‑1 (MQ=255)
                                                                   gCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTc                                                                             >  1:1370635/1‑148 (MQ=255)
                                                                       tCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTCATTTCGCtt                                                                         >  2:2006378/1‑148 (MQ=255)
                                                                       tCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCtt                                                                         <  2:2830709/148‑1 (MQ=255)
                                                                       tCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCtt                                                                         <  2:1215837/148‑1 (MQ=255)
                                                                       tCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAAGTAATTTCGCtt                                                                         >  2:1190829/1‑148 (MQ=255)
                                                                        ccACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTc                                                                        <  1:1702501/148‑1 (MQ=255)
                                                                          aCTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCa                                                                      >  1:1305972/1‑148 (MQ=255)
                                                                             gAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCg                                                                   <  2:2099883/148‑1 (MQ=255)
                                                                              aaaTAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGt                                                                  >  1:225037/1‑148 (MQ=255)
                                                                                  aatCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACg                                                              >  1:2816978/1‑148 (MQ=255)
                                                                                  aatCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACg                                                              <  2:3199411/148‑1 (MQ=255)
                                                                                   atCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACgg                                                             >  2:845697/1‑148 (MQ=255)
                                                                                        aTCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTg                                                        >  2:2987419/1‑148 (MQ=255)
                                                                                        aTCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTg                                                        >  2:2209127/1‑148 (MQ=255)
                                                                                            aCTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTa                                                    >  1:2162189/1‑148 (MQ=255)
                                                                                            aCTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTa                                                    <  2:1081170/148‑1 (MQ=255)
                                                                                            aCTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTa                                                    >  1:1167312/1‑148 (MQ=255)
                                                                                            aCTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTa                                                    <  2:1081178/148‑1 (MQ=255)
                                                                                                ccTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGTTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGcc                                                >  1:1974098/1‑148 (MQ=255)
                                                                                                ccTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGTTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGcc                                                >  1:2205314/1‑148 (MQ=255)
                                                                                                ccTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGcc                                                >  1:2640431/1‑148 (MQ=255)
                                                                                                   gagaAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCAtt                                             >  1:650570/1‑148 (MQ=255)
                                                                                                    agaAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTc                                            <  1:846176/148‑1 (MQ=255)
                                                                                                         cTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGt                                       >  1:744298/1‑148 (MQ=255)
                                                                                                          tGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCCGCGTGACGGGTTTGGTTACGCCATTCAGCGTc                                      <  2:2964391/148‑1 (MQ=255)
                                                                                                          tGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTc                                      <  2:1626368/148‑1 (MQ=255)
                                                                                                          tGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTc                                      <  1:1114046/148‑1 (MQ=255)
                                                                                                          tGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATATTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTc                                      <  2:238299/148‑1 (MQ=255)
                                                                                                            gACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAg                                    >  1:2425122/1‑148 (MQ=255)
                                                                                                            gACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAg                                    >  1:2419202/1‑148 (MQ=255)
                                                                                                             aCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGa                                   >  2:848745/1‑148 (MQ=255)
                                                                                                              ccTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGAt                                  >  2:3044061/1‑148 (MQ=255)
                                                                                                              ccTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGAt                                  >  2:1400564/1‑148 (MQ=255)
                                                                                                                tAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCg                                >  1:798179/1‑148 (MQ=255)
                                                                                                                tAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCg                                <  1:1792571/148‑1 (MQ=255)
                                                                                                                       gTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTaa                         >  2:1480595/1‑148 (MQ=255)
                                                                                                                             atatTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCa                   >  2:518039/1‑148 (MQ=255)
                                                                                                                             atatTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCa                   >  2:3293219/1‑148 (MQ=255)
                                                                                                                              tatTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAg                  >  1:1892733/1‑148 (MQ=255)
                                                                                                                                  gAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCg              >  1:1481168/1‑148 (MQ=255)
                                                                                                                                    aGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTc            >  1:2391641/1‑148 (MQ=255)
                                                                                                                                     gTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCa           <  1:1218244/148‑1 (MQ=255)
                                                                                                                                     gTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACCCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCa           >  1:1540635/1‑148 (MQ=255)
                                                                                                                                      tCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCAc          >  2:2567281/1‑148 (MQ=255)
                                                                                                                                        cTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCg        >  1:3266784/1‑148 (MQ=255)
                                                                                                                                         ttGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGt       >  1:1291245/1‑148 (MQ=255)
                                                                                                                                          tGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGtc      >  2:313458/1‑148 (MQ=255)
                                                                                                                                          tGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGtc      <  2:1801120/148‑1 (MQ=255)
                                                                                                                                          tGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGtc      >  2:2145251/1‑148 (MQ=255)
                                                                                                                                          tGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATACAGTTCGTCACCGtc      >  2:2143654/1‑148 (MQ=255)
                                                                                                                                           gAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGTTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGtct     <  2:1974098/148‑1 (MQ=255)
                                                                                                                                              cTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGtctttc  <  1:667911/148‑1 (MQ=255)
                                                                                                                                              cTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGtctttc  <  2:3001708/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTC  >  CP009273/1113305‑1113594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCTTCAC  >  CP009273/1113303‑1113599
                                                                                                                                                   |                                                                                                                                                     
TCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTAT                                                                                                                                                     >  SRR19973070.1365457/1‑150 (MQ=60)
   AACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACTCTTACATCTGCTGTACGCCTTCCACTTAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTCTCTTGATATTGAAGTCCTTGAGGTTTATTTT                                                                                                                                                  >  SRR19973070.936464/1‑150 (MQ=60)
   AACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTT                                                                                                                                                  >  SRR19973070.521160/1‑150 (MQ=60)
     CAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAgatcgga                                                                                                                                                >  SRR19973070.2349710/1‑143 (MQ=60)
      AGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCC                                                                                                                                               >  SRR19973070.1739643/1‑150 (MQ=60)
      AGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCC                                                                                                                                               >  SRR19973070.1740555/1‑150 (MQ=60)
      AGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCCTCCACTGNAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCC                                                                                                                                               <  SRR19973070.1694900/150‑1 (MQ=60)
      AGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCAGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTGTTGATATTGAATTCCTTGAGCTTTATTTTGCC                                                                                                                                               >  SRR19973070.2741921/1‑150 (MQ=60)
            AATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGC                                                                                                                                         >  SRR19973070.773881/1‑150 (MQ=60)
            AATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGC                                                                                                                                         >  SRR19973070.3220115/1‑150 (MQ=60)
                 CATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGA                                                                                                                                    >  SRR19973070.2976996/1‑150 (MQ=60)
                               TGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTA                                                                                                                      >  SRR19973070.2061524/1‑150 (MQ=60)
                                  CATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCA                                                                                                                   >  SRR19973070.1775257/1‑150 (MQ=60)
                                  CATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCA                                                                                                                   >  SRR19973070.3046596/1‑150 (MQ=60)
                                   ATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCAC                                                                                                                  <  SRR19973070.2166447/150‑1 (MQ=60)
                                      CGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCC                                                                                                               >  SRR19973070.2655292/1‑150 (MQ=60)
                                              TTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCG                                                                                                       <  SRR19973070.1001082/150‑1 (MQ=60)
                                                TCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTC                                                                                                     <  SRR19973070.2908960/150‑1 (MQ=60)
                                                      TTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTG                                                                                               <  SRR19973070.1983218/150‑1 (MQ=60)
                                                      TTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTG                                                                                               <  SRR19973070.2624668/150‑1 (MQ=60)
                                                            CTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAAT                                                                                         <  SRR19973070.2884183/150‑1 (MQ=60)
                                                            CTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAAT                                                                                         <  SRR19973070.2884223/150‑1 (MQ=60)
                                                               CTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAA                                                                                      >  SRR19973070.1600440/1‑150 (MQ=60)
                                                               CTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAA                                                                                      >  SRR19973070.1600119/1‑150 (MQ=60)
                                                                 GTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATT                                                                                    <  SRR19973070.2380774/150‑1 (MQ=60)
                                                                 GTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATT                                                                                    >  SRR19973070.2676699/1‑150 (MQ=60)
                                                                   ACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTC                                                                                  >  SRR19973070.2400596/1‑150 (MQ=60)
                                                                   ACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTC                                                                                  >  SRR19973070.1379959/1‑150 (MQ=60)
                                                                    CGCCGTCCACTGAAATAATCAGATCCACGTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCG                                                                                 <  SRR19973070.3283944/150‑1 (MQ=60)
                                                                          CCACTGACATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCA                                                                           <  SRR19973070.1714371/150‑1 (MQ=60)
                                                                          CCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCA                                                                           <  SRR19973070.1714240/150‑1 (MQ=60)
                                                                          CCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCA                                                                           >  SRR19973070.1315045/1‑150 (MQ=60)
                                                                              TGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGT                                                                       >  SRR19973070.226648/1‑150 (MQ=60)
                                                                                AAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGA                                                                     >  SRR19973070.1619621/1‑150 (MQ=60)
                                                                                AAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGA                                                                     >  SRR19973070.728068/1‑150 (MQ=60)
                                                                                  ATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACG                                                                   >  SRR19973070.2835189/1‑150 (MQ=60)
                                                                                  ATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATAAATTTCGCTTCCAGCGTGACG                                                                   >  SRR19973070.2654922/1‑150 (MQ=60)
                                                                                        AGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCTCTTCCAGCGTGACGGGTTTG                                                             >  SRR19973070.3139278/1‑150 (MQ=60)
                                                                                          ATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGT                                                           >  SRR19973070.2179000/1‑150 (MQ=60)
                                                                                          ATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGT                                                           >  SRR19973070.2487406/1‑150 (MQ=60)
                                                                                            CCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTA                                                         <  SRR19973070.411277/150‑1 (MQ=60)
                                                                                            CCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTA                                                         >  SRR19973070.2176555/1‑150 (MQ=60)
                                                                                            CCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTA                                                         >  SRR19973070.1175486/1‑150 (MQ=60)
                                                                                             CACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTAC                                                        >  SRR19973070.2059420/1‑150 (MQ=60)
                                                                                                TTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGTTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCC                                                     >  SRR19973070.2219837/1‑150 (MQ=60)
                                                                                                TTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGTTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCC                                                     >  SRR19973070.1987446/1‑150 (MQ=60)
                                                                                                TTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCC                                                     >  SRR19973070.2657637/1‑150 (MQ=60)
                                                                                                   CTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATT                                                  >  SRR19973070.655206/1‑150 (MQ=60)
                                                                                                      AGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAG                                               <  SRR19973070.851954/150‑1 (MQ=60)
                                                                                                         AGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGT                                            >  SRR19973070.749560/1‑150 (MQ=60)
                                                                                                            TGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAG                                         <  SRR19973070.1121658/150‑1 (MQ=60)
                                                                                                            TGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAG                                         >  SRR19973070.2441063/1‑150 (MQ=60)
                                                                                                            TGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAG                                         >  SRR19973070.2435117/1‑150 (MQ=60)
                                                                                                                CCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCG                                     >  SRR19973070.803686/1‑150 (MQ=60)
                                                                                                                  TAATTGTGTCTTGATATTGAAGTACTTGAGCTTTATTTTTCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATCGGTCGTCGCCCTGAACAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCC                                   <  SRR19973070.1806347/150‑1 (MQ=60)
                                                                                                                  TAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCC                                   <  SRR19973070.1804956/150‑1 (MQ=60)
                                                                                                                    AATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGG                                 >  SRR19973070.851468/1‑150 (MQ=60)
                                                                                                                       CTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAA                              <  SRR19973070.3229762/150‑1 (MQ=60)
                                                                                                                          TCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATAT                           <  SRR19973070.1193240/150‑1 (MQ=60)
                                                                                                                           CTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATC                          >  SRR19973070.762971/1‑150 (MQ=60)
                                                                                                                              GATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAG                       >  SRR19973070.1905634/1‑150 (MQ=60)
                                                                                                                              GATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAG                       >  SRR19973070.1905564/1‑150 (MQ=60)
                                                                                                                              GATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAG                       >  SRR19973070.59254/1‑150 (MQ=60)
                                                                                                                               ATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGT                      >  SRR19973070.2101853/1‑150 (MQ=60)
                                                                                                                                  TTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCG                   >  SRR19973070.1491122/1‑150 (MQ=60)
                                                                                                                                  TTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCG                   >  SRR19973070.2670265/1‑150 (MQ=60)
                                                                                                                                  TTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCG                   <  SRR19973070.1288380/150‑1 (MQ=60)
                                                                                                                                  TTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCG                   >  SRR19973070.1491538/1‑150 (MQ=60)
                                                                                                                                    GAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTC                 >  SRR19973070.242876/1‑150 (MQ=60)
                                                                                                                                    GAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTC                 >  SRR19973070.2407429/1‑150 (MQ=60)
                                                                                                                                     AAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCA                <  SRR19973070.2251030/150‑1 (MQ=60)
                                                                                                                                     AAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACCCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCA                >  SRR19973070.1551460/1‑150 (MQ=60)
                                                                                                                                      AGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCAC               <  SRR19973070.2734069/150‑1 (MQ=60)
                                                                                                                                       GTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACC              <  SRR19973070.1226784/150‑1 (MQ=60)
                                                                                                                                        TCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCG             >  SRR19973070.3287806/1‑150 (MQ=60)
                                                                                                                                         CCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGT            >  SRR19973070.1300245/1‑150 (MQ=60)
                                                                                                                                                CTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCTT     <  SRR19973070.672679/150‑1 (MQ=60)
                                                                                                                                                 TTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCTTC    <  SRR19973070.3100080/150‑1 (MQ=60)
                                                                                                                                                   TATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCTTCAC  >  SRR19973070.2641147/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
TCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCTTCAC  >  CP009273/1113303‑1113599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑