Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,113,450 A→T 100% I161I (ATT→ATA)  yceI ← secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,113,4500AT97.3% 713.9 / 7.0 220I161I (ATT→ATA) yceIsecreted protein
Reads supporting (aligned to +/- strand):  ref base A (2/4);  new base T (135/79);  total (137/83)
Fisher's exact test for biased strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 220 total aligned reads displayed.

CAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTT  >  CP009273/1113305‑1113593
                                                                                                                                                 |                                                                                                                                               
cAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTAt                                                                                                                                               <  2:1016386/148‑1 (MQ=255)
 aaCAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTAtt                                                                                                                                              >  2:785512/1‑148 (MQ=255)
  aCAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTAttt                                                                                                                                             >  1:2301371/1‑148 (MQ=255)
  aCAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTAttt                                                                                                                                             >  2:527402/1‑148 (MQ=255)
   cAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAATTCTGTCTTGATCTTGAAGTCCTTGAGCTTTAtttt                                                                                                                                            >  2:2888001/1‑148 (MQ=255)
    aGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTg                                                                                                                                           >  2:3661788/1‑148 (MQ=255)
     gAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGACGTCCGTGAGCTTTATTTTGc                                                                                                                                          >  2:3328354/1‑148 (MQ=255)
      aataaataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGcc                                                                                                                                         <  1:213918/148‑1 (MQ=255)
      aataaataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGcc                                                                                                                                         >  1:2762964/1‑148 (MQ=255)
       ataaataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCt                                                                                                                                        >  2:342867/1‑148 (MQ=255)
        taaataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCtt                                                                                                                                       <  2:1572658/148‑1 (MQ=255)
        taaataTCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCtt                                                                                                                                       >  1:114724/1‑148 (MQ=255)
           atatCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCgg                                                                                                                                    <  2:1896902/148‑1 (MQ=255)
                aTTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCg                                                                                                                               >  2:1743945/1‑148 (MQ=255)
                 ttGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGa                                                                                                                              <  1:2702924/148‑1 (MQ=255)
                 ttGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGa                                                                                                                              >  2:3728692/1‑148 (MQ=255)
                 ttGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGa                                                                                                                              <  1:3117749/148‑1 (MQ=255)
                    ccGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGc                                                                                                                           <  1:2265789/148‑1 (MQ=255)
                         tGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCa                                                                                                                      >  1:2876308/1‑148 (MQ=255)
                             tGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGtt                                                                                                                  >  1:1460669/1‑148 (MQ=255)
                               ccATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTa                                                                                                                >  2:2885610/1‑148 (MQ=255)
                                cATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTac                                                                                                               >  2:746039/1‑148 (MQ=255)
                                   ccGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTaccac                                                                                                            >  2:3277025/1‑148 (MQ=255)
                                    cGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTaccacc                                                                                                           <  1:2018088/148‑1 (MQ=255)
                                       cAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCa                                                                                                        >  1:622611/1‑148 (MQ=255)
                                       cAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCa                                                                                                        >  2:3586047/1‑148 (MQ=255)
                                       cAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCa                                                                                                        >  1:1762231/1‑148 (MQ=255)
                                       cAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCa                                                                                                        >  1:3254729/1‑148 (MQ=255)
                                       cAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCa                                                                                                        >  1:1350459/1‑148 (MQ=255)
                                          cATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATgg                                                                                                     >  1:2737795/1‑148 (MQ=255)
                                           aTTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATggg                                                                                                    >  2:1124087/1‑148 (MQ=255)
                                                aCGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGgtcgtc                                                                                               >  2:1372052/1‑148 (MQ=255)
                                                aCGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGgtcgtc                                                                                               >  2:1372344/1‑148 (MQ=255)
                                                   cTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGcc                                                                                            >  2:2000309/1‑148 (MQ=255)
                                                   cTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGcc                                                                                            >  1:2666027/1‑148 (MQ=255)
                                                   cTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGcc                                                                                            >  2:3020852/1‑148 (MQ=255)
                                                   cTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCAATTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGcc                                                                                            <  1:112876/148‑1 (MQ=255)
                                                       cTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGa                                                                                        <  2:443966/148‑1 (MQ=255)
                                                                 aCGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCaattaatt                                                                              >  2:2124155/1‑148 (MQ=255)
                                                                      ttCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCt                                                                         <  1:3326711/148‑1 (MQ=255)
                                                                      ttCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCt                                                                         >  1:1519155/1‑148 (MQ=255)
                                                                       tCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCtt                                                                        >  1:2417801/1‑148 (MQ=255)
                                                                        ccACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTc                                                                       <  1:608693/148‑1 (MQ=255)
                                                                             gAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCg                                                                  >  2:2837510/1‑148 (MQ=255)
                                                                             gAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCg                                                                  >  2:2560106/1‑148 (MQ=255)
                                                                             gAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCg                                                                  <  1:1062120/148‑1 (MQ=255)
                                                                              aaaTAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGt                                                                 >  2:1505277/1‑148 (MQ=255)
                                                                               aataatCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTg                                                                <  1:89978/148‑1 (MQ=255)
                                                                                ataatCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGTCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGa                                                               <  2:2161913/148‑1 (MQ=255)
                                                                                   atCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACgg                                                            >  2:2417096/1‑148 (MQ=255)
                                                                                    tCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACggg                                                           <  1:713403/148‑1 (MQ=255)
                                                                                    tCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACggg                                                           >  1:3722476/1‑148 (MQ=255)
                                                                                    tCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACggg                                                           <  1:3593782/148‑1 (MQ=255)
                                                                                     cAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGt                                                          <  1:1268494/148‑1 (MQ=255)
                                                                                      aGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGtt                                                         >  2:3495017/1‑148 (MQ=255)
                                                                                       gATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGttt                                                        >  2:3047427/1‑148 (MQ=255)
                                                                                        aTCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTTAATTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTAGCCCTGACCAATTAATATCGCTTCCAGAGTGACGGGTTTg                                                       >  1:148688/1‑148 (MQ=255)
                                                                                        aTCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTg                                                       >  1:2465575/1‑148 (MQ=255)
                                                                                        aTCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTg                                                       >  1:2472175/1‑148 (MQ=255)
                                                                                        aTCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTg                                                       >  1:2615238/1‑148 (MQ=255)
                                                                                         tCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTgg                                                      >  2:1798807/1‑148 (MQ=255)
                                                                                         tCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTgg                                                      <  1:2825213/148‑1 (MQ=255)
                                                                                          ccACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGt                                                     <  1:3052387/148‑1 (MQ=255)
                                                                                           cACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGtt                                                    >  2:928255/1‑148 (MQ=255)
                                                                                            aCTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCACTTAATTTCGCTTCCAGCGTGACGGGTTTGGTTa                                                   <  2:2310849/148‑1 (MQ=255)
                                                                                               tCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGc                                                >  2:2449986/1‑148 (MQ=255)
                                                                                               tCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGc                                                >  1:3213253/1‑148 (MQ=255)
                                                                                                    agaAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTc                                           <  1:818443/148‑1 (MQ=255)
                                                                                                    agaAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTc                                           >  2:2537912/1‑148 (MQ=255)
                                                                                                        gCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCg                                       >  1:1978774/1‑148 (MQ=255)
                                                                                                        gCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCg                                       >  1:216862/1‑148 (MQ=255)
                                                                                                        gCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCg                                       >  1:217259/1‑148 (MQ=255)
                                                                                                        gCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCAGTCAGCg                                       >  1:1978705/1‑148 (MQ=255)
                                                                                                         cTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGt                                      >  2:2668265/1‑148 (MQ=255)
                                                                                                            gACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAg                                   >  2:571400/1‑148 (MQ=255)
                                                                                                             aCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGa                                  <  1:1273699/148‑1 (MQ=255)
                                                                                                             aCCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGa                                  <  1:1703595/148‑1 (MQ=255)
                                                                                                              ccTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGAt                                 >  2:1857646/1‑148 (MQ=255)
                                                                                                                tAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCg                               >  2:2798899/1‑148 (MQ=255)
                                                                                                                  aaTCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGcc                             >  1:518002/1‑148 (MQ=255)
                                                                                                                   aTCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCg                            >  1:3505767/1‑148 (MQ=255)
                                                                                                                      tGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTa                         <  1:917650/148‑1 (MQ=255)
                                                                                                                        tCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAat                       >  1:708701/1‑148 (MQ=255)
                                                                                                                        tCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAat                       >  1:2888981/1‑148 (MQ=255)
                                                                                                                        tCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAat                       >  1:2347949/1‑148 (MQ=255)
                                                                                                                             atatTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCa                  >  2:488908/1‑148 (MQ=255)
                                                                                                                              tatTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAg                 <  2:611939/148‑1 (MQ=255)
                                                                                                                              tatTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAg                 <  2:2358028/148‑1 (MQ=255)
                                                                                                                               atTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGTTTACGCCATTCAGAGTCAGATCGCCGTTAATATCCAGt                >  1:181090/1‑148 (MQ=255)
                                                                                                                               atTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGt                >  1:505653/1‑148 (MQ=255)
                                                                                                                               atTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGt                <  1:746045/148‑1 (MQ=255)
                                                                                                                                ttGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGtt               >  2:3162503/1‑148 (MQ=255)
                                                                                                                                ttGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGtt               >  1:2190901/1‑148 (MQ=255)
                                                                                                                                 tGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTc              >  1:837577/1‑148 (MQ=255)
                                                                                                                                     gTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCa          <  2:505056/148‑1 (MQ=255)
                                                                                                                                      tCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCAc         >  1:3125373/1‑148 (MQ=255)
                                                                                                                                          tGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGtc     <  1:2850492/148‑1 (MQ=255)
                                                                                                                                             gCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGtcttt  >  2:3300654/1‑148 (MQ=255)
                                                                                                                                             gCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGtcttt  <  1:670422/148‑1 (MQ=255)
                                                                                                                                             gCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGtcttt  >  2:1400656/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTT  >  CP009273/1113305‑1113593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCTT  >  CP009273/1113301‑1113596
                                                                                                                                                     |                                                                                                                                                  
GATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAATTCTGTCTTGATATTGAAGTCCTTGAGCTTT                                                                                                                                                    >  SRR19973069.3647060/1‑150 (MQ=60)
GATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTT                                                                                                                                                    >  SRR19973069.184711/1‑150 (MQ=60)
GATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTT                                                                                                                                                    <  SRR19973069.3760171/150‑1 (MQ=60)
GATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTT                                                                                                                                                    >  SRR19973069.3647041/1‑150 (MQ=60)
  TCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTAT                                                                                                                                                  <  SRR19973069.1656858/150‑1 (MQ=60)
    CAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTT                                                                                                                                                >  SRR19973069.2317019/1‑150 (MQ=60)
    CAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTT                                                                                                                                                >  SRR19973069.2797264/1‑150 (MQ=60)
     AACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTT                                                                                                                                               >  SRR19973069.882711/1‑150 (MQ=60)
     AACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTT                                                                                                                                               >  SRR19973069.2808395/1‑150 (MQ=60)
     AACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTNT                                                                                                                                               <  SRR19973069.2602284/150‑1 (MQ=60)
        AGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCC                                                                                                                                            >  SRR19973069.2643881/1‑150 (MQ=60)
        AGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCC                                                                                                                                            >  SRR19973069.2781404/1‑150 (MQ=60)
          AATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTT                                                                                                                                          <  SRR19973069.215598/150‑1 (MQ=60)
          AATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTT                                                                                                                                          >  SRR19973069.115847/1‑150 (MQ=60)
             AAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGG                                                                                                                                       >  SRR19973069.2703384/1‑150 (MQ=60)
             AAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGG                                                                                                                                       >  SRR19973069.2377770/1‑150 (MQ=60)
                  TCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCG                                                                                                                                  <  SRR19973069.3442041/150‑1 (MQ=60)
                     TTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAG                                                                                                                               <  SRR19973069.2721043/150‑1 (MQ=60)
                     TTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAG                                                                                                                               <  SRR19973069.3138314/150‑1 (MQ=60)
                     TTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAG                                                                                                                               <  SRR19973069.3138235/150‑1 (MQ=60)
                        CCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCT                                                                                                                            <  SRR19973069.1719254/150‑1 (MQ=60)
                        CCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCT                                                                                                                            <  SRR19973069.2281177/150‑1 (MQ=60)
                        CCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCT                                                                                                                            <  SRR19973069.2281138/150‑1 (MQ=60)
                         CGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTG                                                                                                                           >  SRR19973069.2115651/1‑150 (MQ=60)
                           GATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCA                                                                                                                         >  SRR19973069.2895179/1‑150 (MQ=60)
                               CGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTT                                                                                                                     >  SRR19973069.1470858/1‑150 (MQ=60)
                                GTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTT                                                                                                                    <  SRR19973069.1136726/150‑1 (MQ=60)
                                   CCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCCTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACC                                                                                                                 <  SRR19973069.651037/150‑1 (MQ=60)
                                    CATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCA                                                                                                                >  SRR19973069.2332056/1‑150 (MQ=60)
                                      TCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACC                                                                                                              <  SRR19973069.1790794/150‑1 (MQ=60)
                                        CGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCC                                                                                                            <  SRR19973069.2031999/150‑1 (MQ=60)
                                        CGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCC                                                                                                            <  SRR19973069.2032123/150‑1 (MQ=60)
                                         GGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCA                                                                                                           >  SRR19973069.1774657/1‑150 (MQ=60)
                                         GGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCA                                                                                                           >  SRR19973069.1359812/1‑150 (MQ=60)
                                         GGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCA                                                                                                           >  SRR19973069.3276046/1‑150 (MQ=60)
                                         GGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCA                                                                                                           >  SRR19973069.3276064/1‑150 (MQ=60)
                                         GGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCA                                                                                                           >  SRR19973069.626749/1‑150 (MQ=60)
                                           CAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATG                                                                                                         >  SRR19973069.701782/1‑150 (MQ=60)
                                            AACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGG                                                                                                        >  SRR19973069.2756106/1‑150 (MQ=60)
                                                  TCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTC                                                                                                  >  SRR19973069.616029/1‑150 (MQ=60)
                                                   CACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCG                                                                                                 <  SRR19973069.1117352/150‑1 (MQ=60)
                                                   CACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCG                                                                                                 <  SRR19973069.1413865/150‑1 (MQ=60)
                                                     CGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCC                                                                                               >  SRR19973069.2683900/1‑150 (MQ=60)
                                                      GCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCC                                                                                              >  SRR19973069.575777/1‑150 (MQ=60)
                                                       CTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCAATTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCT                                                                                             <  SRR19973069.113980/150‑1 (MQ=60)
                                                            TTCTGCTGTACGCCTTCCACTGAAATAATCAGCTCCACGTCCTGAGAAGCTTGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCA                                                                                        <  SRR19973069.3231778/150‑1 (MQ=60)
                                                                GCTTTACGCCTTCCACTTAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTATTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCTAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTA                                                                                    <  SRR19973069.3469258/150‑1 (MQ=60)
                                                                        CCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCT                                                                            >  SRR19973069.1529637/1‑150 (MQ=60)
                                                                         CTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTT                                                                           >  SRR19973069.2434133/1‑150 (MQ=60)
                                                                          TTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTC                                                                          <  SRR19973069.3348514/150‑1 (MQ=60)
                                                                            CCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCA                                                                        <  SRR19973069.612775/150‑1 (MQ=60)
                                                                             CACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAG                                                                       <  SRR19973069.2240303/150‑1 (MQ=60)
                                                                               CTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCG                                                                     >  SRR19973069.3532971/1‑150 (MQ=60)
                                                                                 GAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTG                                                                   <  SRR19973069.1069519/150‑1 (MQ=60)
                                                                                  AAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGA                                                                  <  SRR19973069.3116706/150‑1 (MQ=60)
                                                                                   AATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGAC                                                                 <  SRR19973069.90913/150‑1 (MQ=60)
                                                                                      AATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGG                                                              >  SRR19973069.3746773/1‑150 (MQ=60)
                                                                                        TCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTT                                                            <  SRR19973069.3617242/150‑1 (MQ=60)
                                                                                        TCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTT                                                            <  SRR19973069.718424/150‑1 (MQ=60)
                                                                                         CAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTT                                                           <  SRR19973069.1277146/150‑1 (MQ=60)
                                                                                         CAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTT                                                           <  SRR19973069.1277025/150‑1 (MQ=60)
                                                                                          AGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTTAATTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTAGCCCTGACCAATTAATATCGCTTCCAGAGTGACGGGTTTG                                                          >  SRR19973069.150010/1‑150 (MQ=60)
                                                                                          AGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTG                                                          >  SRR19973069.2632717/1‑150 (MQ=60)
                                                                                          AGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTG                                                          >  SRR19973069.922404/1‑150 (MQ=60)
                                                                                          AGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTG                                                          >  SRR19973069.2488758/1‑150 (MQ=60)
                                                                                          AGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTG                                                          >  SRR19973069.2482126/1‑150 (MQ=60)
                                                                                          AGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTG                                                          >  SRR19973069.150009/1‑150 (MQ=60)
                                                                                          AGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTG                                                          >  SRR19973069.2484959/1‑150 (MQ=60)
                                                                                           GATCCACGTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGG                                                         <  SRR19973069.1303708/150‑1 (MQ=60)
                                                                                             TCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTT                                                       <  SRR19973069.2843897/150‑1 (MQ=60)
                                                                                              CCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTA                                                      <  SRR19973069.3072585/150‑1 (MQ=60)
                                                                                              CCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTA                                                      <  SRR19973069.3072607/150‑1 (MQ=60)
                                                                                              CCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTA                                                      >  SRR19973069.714710/1‑150 (MQ=60)
                                                                                                 CTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGC                                                   >  SRR19973069.3234223/1‑150 (MQ=60)
                                                                                                 CTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGC                                                   >  SRR19973069.3234214/1‑150 (MQ=60)
                                                                                                      TGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTC                                              >  SRR19973069.1284435/1‑150 (MQ=60)
                                                                                                      TGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTC                                              <  SRR19973069.2446928/150‑1 (MQ=60)
                                                                                                        AGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAG                                            <  SRR19973069.824224/150‑1 (MQ=60)
                                                                                                          AAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCG                                          >  SRR19973069.218957/1‑150 (MQ=60)
                                                                                                          AAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCG                                          >  SRR19973069.1992537/1‑150 (MQ=60)
                                                                                                          AAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCG                                          >  SRR19973069.218859/1‑150 (MQ=60)
                                                                                                          AAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCG                                          >  SRR19973069.218557/1‑150 (MQ=60)
                                                                                                          AAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCG                                          >  SRR19973069.1992497/1‑150 (MQ=60)
                                                                                                          AAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCG                                          >  SRR19973069.1521066/1‑150 (MQ=60)
                                                                                                          AAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCAGTCAGCG                                          >  SRR19973069.1992468/1‑150 (MQ=60)
                                                                                                                GACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGAT                                    >  SRR19973069.2939406/1‑150 (MQ=60)
                                                                                                                 ACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATC                                   <  SRR19973069.1715514/150‑1 (MQ=60)
                                                                                                                 ACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATC                                   <  SRR19973069.1282250/150‑1 (MQ=60)
                                                                                                                    TAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCC                                >  SRR19973069.521683/1‑150 (MQ=60)
                                                                                                                     AAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCG                               >  SRR19973069.3528326/1‑150 (MQ=60)
                                                                                                                     AAATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCG                               >  SRR19973069.2756504/1‑150 (MQ=60)
                                                                                                                      AATCTGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGG                              <  SRR19973069.3685761/150‑1 (MQ=60)
                                                                                                                          TGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAAT                          <  SRR19973069.923985/150‑1 (MQ=60)
                                                                                                                          TGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAAT                          <  SRR19973069.923917/150‑1 (MQ=60)
                                                                                                                          TGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAAT                          <  SRR19973069.923907/150‑1 (MQ=60)
                                                                                                                          TGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAAT                          >  SRR19973069.2908021/1‑150 (MQ=60)
                                                                                                                          TGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAAT                          >  SRR19973069.2907985/1‑150 (MQ=60)
                                                                                                                          TGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAAT                          <  SRR19973069.1264627/150‑1 (MQ=60)
                                                                                                                          TGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAAT                          >  SRR19973069.426469/1‑150 (MQ=60)
                                                                                                                          TGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAAT                          >  SRR19973069.713678/1‑150 (MQ=60)
                                                                                                                          TGTCTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAAT                          >  SRR19973069.2363922/1‑150 (MQ=60)
                                                                                                                             CTTGATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATC                       >  SRR19973069.1740270/1‑150 (MQ=60)
                                                                                                                                 ATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGTTTACGCCATTCAGAGTCAGATCGCCGTTAATATCCAGT                   >  SRR19973069.182598/1‑150 (MQ=60)
                                                                                                                                 ATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGT                   >  SRR19973069.509267/1‑150 (MQ=60)
                                                                                                                                 ATATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGT                   >  SRR19973069.509586/1‑150 (MQ=60)
                                                                                                                                  TATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTT                  >  SRR19973069.2205808/1‑150 (MQ=60)
                                                                                                                                  TATTGAAGACCATGAGCTTTATTTTGCCTTCGGCCTCGAAGACAGAACGATTACCACCCCATGGGTCCACGCCCTGACCAATAAATTTCGCTTCCAGCGTGACGGGTATGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATACAGTT                  >  SRR19973069.2205758/1‑150 (MQ=60)
                                                                                                                                   ATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTC                 <  SRR19973069.2305995/150‑1 (MQ=60)
                                                                                                                                   ATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTC                 >  SRR19973069.843462/1‑150 (MQ=60)
                                                                                                                                   ATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTC                 <  SRR19973069.751331/150‑1 (MQ=60)
                                                                                                                                   ATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTC                 <  SRR19973069.751325/150‑1 (MQ=60)
                                                                                                                                   ATTGAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTC                 <  SRR19973069.751306/150‑1 (MQ=60)
                                                                                                                                      GAAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTC              >  SRR19973069.1611257/1‑150 (MQ=60)
                                                                                                                                       AAGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCA             <  SRR19973069.1891646/150‑1 (MQ=60)
                                                                                                                                        AGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCAC            >  SRR19973069.2754065/1‑150 (MQ=60)
                                                                                                                                        AGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCAC            >  SRR19973069.3145887/1‑150 (MQ=60)
                                                                                                                                        AGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCAC            <  SRR19973069.575048/150‑1 (MQ=60)
                                                                                                                                        AGTCCTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCAC            >  SRR19973069.825981/1‑150 (MQ=60)
                                                                                                                                            CTTGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTC        <  SRR19973069.2506628/150‑1 (MQ=60)
                                                                                                                                              TGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTT      <  SRR19973069.2869256/150‑1 (MQ=60)
                                                                                                                                              TGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTT      <  SRR19973069.2869381/150‑1 (MQ=60)
                                                                                                                                              TGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTT      <  SRR19973069.2869263/150‑1 (MQ=60)
                                                                                                                                              TGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTT      >  SRR19973069.803966/1‑150 (MQ=60)
                                                                                                                                              TGAGCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTT      <  SRR19973069.2063601/150‑1 (MQ=60)
                                                                                                                                                 GCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCT   <  SRR19973069.2171124/150‑1 (MQ=60)
                                                                                                                                                 GCTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCT   <  SRR19973069.675023/150‑1 (MQ=60)
                                                                                                                                                  CTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCTT  >  SRR19973069.3261789/1‑150 (MQ=60)
                                                                                                                                                  CTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCTT  >  SRR19973069.3207417/1‑150 (MQ=60)
                                                                                                                                                  CTTTATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCTT  >  SRR19973069.3207432/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
GATCCAACAGAATAAATATCATTGCCGGATGCGTGCCATCCGGCAACATTTCACGCTTACTTCTGCTGTACGCCTTCCACTGAAATAATCAGATCCACTTCCTGAGAAGCTGGACCTAAATCTGTCTTGATATTGAAGTCCTTGAGCTTAATTTTGCCTTCGGCCTCGAAGCCTGCACGTTTACCACCCCATGGGTCGTCGCCCTGACCAATTAATTTCGCTTCCAGCGTGACGGGTTTGGTTACGCCATTCAGCGTCAGATCGCCGGTAATATCCAGTTCGTCACCGTCTTTCTT  >  CP009273/1113301‑1113596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑