Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,114,329 C→A 100% L58F (TTG→TTT)  yceJ ← putative cytochrome b561

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,114,3290CA88.4% 418.7 / 53.6 172L58F (TTG→TTT) yceJputative cytochrome b561
Reads supporting (aligned to +/- strand):  ref base C (14/6);  new base A (75/77);  total (89/83)
Fisher's exact test for biased strand distribution p-value = 9.85e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 174 total aligned reads displayed.

CTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACC  >  CP009273/1114183‑1114472
                                                                                                                                                  |                                                                                                                                               
cTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACa                                                                                                                                                >  2:848009/1‑148 (MQ=255)
 tGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAaaaa                                                                                                                                               <  1:2987777/148‑1 (MQ=255)
    tGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAAt                                                                                                                                            <  2:2427802/148‑1 (MQ=255)
        gATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCg                                                                                                                                        <  1:1725102/148‑1 (MQ=255)
            gCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGc                                                                                                                                    >  2:656359/1‑148 (MQ=255)
            gCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGc                                                                                                                                    >  2:656440/1‑148 (MQ=255)
            gCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGc                                                                                                                                    <  2:3176999/148‑1 (MQ=255)
             cGAAAAGCAATAGGTACAATGCTAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCTAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCt                                                                                                                                   <  2:2106593/148‑1 (MQ=255)
             cGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCt                                                                                                                                   <  2:2091738/148‑1 (MQ=255)
               aaaaGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCttt                                                                                                                                 >  2:2005959/1‑148 (MQ=255)
               aaaaGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCttt                                                                                                                                 <  1:542875/148‑1 (MQ=255)
                aaaGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCtttt                                                                                                                                <  2:1568281/148‑1 (MQ=255)
                aaaGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCtttt                                                                                                                                >  2:1090284/1‑148 (MQ=255)
                aaaGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCAGCATTAAAAAAATACCGATGCtttt                                                                                                                                >  2:1747417/1‑148 (MQ=255)
                 aaGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTa                                                                                                                               >  1:2756324/1‑148 (MQ=255)
                 aaGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTa                                                                                                                               <  1:880989/148‑1 (MQ=255)
                  aGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTAt                                                                                                                              >  1:2394866/1‑148 (MQ=255)
                  aGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTAt                                                                                                                              >  2:761050/1‑148 (MQ=255)
                   gCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATg                                                                                                                             <  2:710025/148‑1 (MQ=255)
                    cAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGc                                                                                                                            >  2:730671/1‑148 (MQ=255)
                      aTAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAg                                                                                                                          <  1:2697458/148‑1 (MQ=255)
                        aGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGtt                                                                                                                        <  1:2536559/148‑1 (MQ=255)
                        aGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGtt                                                                                                                        <  2:2393075/148‑1 (MQ=255)
                        aGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGtt                                                                                                                        <  1:650926/148‑1 (MQ=255)
                        aGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGtt                                                                                                                        >  2:2077062/1‑148 (MQ=255)
                        aGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGtt                                                                                                                        <  1:2188907/148‑1 (MQ=255)
                        aGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGtt                                                                                                                        >  2:412924/1‑148 (MQ=255)
                           tACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCgg                                                                                                                     <  1:1698578/148‑1 (MQ=255)
                            aCAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCggg                                                                                                                    <  1:1515960/148‑1 (MQ=255)
                            aCAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCggg                                                                                                                    >  2:2370794/1‑148 (MQ=255)
                             cAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGt                                                                                                                   >  1:2164008/1‑148 (MQ=255)
                             cAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGt                                                                                                                   >  1:1272367/1‑148 (MQ=255)
                              aaTGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTg                                                                                                                  >  2:171738/1‑148 (MQ=255)
                               aTGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGc                                                                                                                 >  2:2429953/1‑148 (MQ=255)
                                tGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATTACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCt                                                                                                                <  1:2488621/148‑1 (MQ=255)
                                tGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCt                                                                                                                >  2:1592850/1‑148 (MQ=255)
                                 gCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCtt                                                                                                               >  1:2264985/1‑148 (MQ=255)
                                 gCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCtt                                                                                                               <  1:164115/148‑1 (MQ=255)
                                   gagaTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTtg                                                                                                             <  1:2061022/148‑1 (MQ=255)
                                      aTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTCACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTGTGTGa                                                                                                          >  2:3313104/1‑148 (MQ=255)
                                      aTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGa                                                                                                          >  2:3066369/1‑148 (MQ=255)
                                         tCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATAc                                                                                                       <  2:1784681/148‑1 (MQ=255)
                                           cAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCa                                                                                                     >  2:1875731/1‑148 (MQ=255)
                                              ctctTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGcc                                                                                                  >  1:1414528/1‑148 (MQ=255)
                                              ctcgTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGcc                                                                                                  <  2:1878593/148‑1 (MQ=255)
                                                ctTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAANGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCAt                                                                                                >  1:1679077/1‑148 (MQ=255)
                                                ctTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCAt                                                                                                >  2:397423/1‑148 (MQ=255)
                                                  tGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCg                                                                                              >  2:735197/1‑148 (MQ=255)
                                                  tGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCg                                                                                              >  2:267918/1‑148 (MQ=255)
                                                      gCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGtaat                                                                                          <  1:1123731/148‑1 (MQ=255)
                                                          gACGAGTCATGGGCGAATAACTCGTCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACt                                                                                      <  2:129773/148‑1 (MQ=255)
                                                           aCGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTg                                                                                     >  1:1062342/1‑148 (MQ=255)
                                                             gAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAg                                                                                   <  1:2438413/148‑1 (MQ=255)
                                                               gTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCg                                                                                 <  1:2929998/148‑1 (MQ=255)
                                                               gTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCg                                                                                 <  1:2030552/148‑1 (MQ=255)
                                                               gTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCg                                                                                 <  1:2030472/148‑1 (MQ=255)
                                                                 cATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTg                                                                               >  2:1680121/1‑148 (MQ=255)
                                                                    gggCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGAcc                                                                            <  1:759846/148‑1 (MQ=255)
                                                                          aTAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCcac                                                                      >  1:2084715/1‑148 (MQ=255)
                                                                          aTAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCAACATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCcac                                                                      <  2:1232230/148‑1 (MQ=255)
                                                                             aCTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGa                                                                   >  2:2638093/1‑148 (MQ=255)
                                                                              cTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGAc                                                                  <  2:1792240/148‑1 (MQ=255)
                                                                                cGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCt                                                                >  2:2224658/1‑148 (MQ=255)
                                                                                         aCCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAAc                                                       <  1:942362/148‑1 (MQ=255)
                                                                                         aCCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAAc                                                       >  1:3010715/1‑148 (MQ=255)
                                                                                          cccGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACa                                                      <  1:736246/148‑1 (MQ=255)
                                                                                                     agaTATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACa                                           >  2:3089629/1‑148 (MQ=255)
                                                                                                     agaTATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACa                                           <  1:1848719/148‑1 (MQ=255)
                                                                                                      gaTATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACaa                                          >  1:1928353/1‑148 (MQ=255)
                                                                                                      gaTATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACaa                                          <  1:3256782/148‑1 (MQ=255)
                                                                                                        tatGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACaata                                        >  1:2963543/1‑148 (MQ=255)
                                                                                                         atGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACaataa                                       >  1:210965/1‑148 (MQ=255)
                                                                                                         atGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACaataa                                       >  2:135734/1‑148 (MQ=255)
                                                                                                          tGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACaataat                                      <  2:3072257/148‑1 (MQ=255)
                                                                                                             cacGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTgc                                   >  1:2643542/1‑148 (MQ=255)
                                                                                                              acGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAATCAAACATGCCGTAGACAATAATTgcg                                  <  1:692759/148‑1 (MQ=255)
                                                                                                                gCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCt                                <  2:1551223/148‑1 (MQ=255)
                                                                                                                      gAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCa                          <  1:1373969/148‑1 (MQ=255)
                                                                                                                      gAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCa                          <  2:966373/148‑1 (MQ=255)
                                                                                                                            gAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGaa                    <  1:2773357/148‑1 (MQ=255)
                                                                                                                             aaTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAg                   >  2:62272/1‑148 (MQ=255)
                                                                                                                              aTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAgg                  >  1:2521522/1‑148 (MQ=255)
                                                                                                                              aTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAgg                  >  2:2814766/1‑148 (MQ=255)
                                                                                                                              aTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAgg                  <  1:2944447/148‑1 (MQ=255)
                                                                                                                              aTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAgg                  <  2:18037/148‑1 (MQ=255)
                                                                                                                              aTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAgg                  <  1:508829/148‑1 (MQ=255)
                                                                                                                               tAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGc                 >  2:36465/1‑148 (MQ=255)
                                                                                                                                aaCCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGcc                >  1:1458005/1‑148 (MQ=255)
                                                                                                                                 aCCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCg               <  2:1924799/148‑1 (MQ=255)
                                                                                                                                  ccAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGc              >  2:2672913/1‑148 (MQ=255)
                                                                                                                                     gCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGa           <  1:822988/148‑1 (MQ=255)
                                                                                                                                      ccccATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGat          >  1:1753542/1‑148 (MQ=255)
                                                                                                                                        ccATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGatat        >  2:2554042/1‑148 (MQ=255)
                                                                                                                                        ccATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGatat        <  1:85561/148‑1 (MQ=255)
                                                                                                                                           tcatTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAac     <  1:2105604/148‑1 (MQ=255)
                                                                                                                                           tcatTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAac     >  2:2363690/1‑148 (MQ=255)
                                                                                                                                              ttAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACAcc  <  2:2320069/148‑1 (MQ=255)
                                                                                                                                              ttAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACAcc  <  1:1012629/148‑1 (MQ=255)
                                                                                                                                              ttAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACAcc  <  2:1101458/148‑1 (MQ=255)
                                                                                                                                              ttAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACAAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAAAAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACAcc  >  1:3162534/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACC  >  CP009273/1114183‑1114472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGC  >  CP009273/1114184‑1114477
                                                                                                                                                 |                                                                                                                                                    
TGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAA                                                                                                                                                  >  SRR19973070.116027/1‑150 (MQ=60)
TGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAA                                                                                                                                                  <  SRR19973070.3006925/150‑1 (MQ=60)
       GATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGAT                                                                                                                                           <  SRR19973070.1737045/150‑1 (MQ=60)
          GGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCT                                                                                                                                        <  SRR19973070.2860012/150‑1 (MQ=60)
          GGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCT                                                                                                                                        >  SRR19973070.826973/1‑150 (MQ=60)
            CGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTT                                                                                                                                      >  SRR19973070.540091/1‑150 (MQ=60)
              AAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTA                                                                                                                                    >  SRR19973070.2774013/1‑150 (MQ=60)
              AAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTA                                                                                                                                    <  SRR19973070.546499/150‑1 (MQ=60)
               AAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTAT                                                                                                                                   >  SRR19973070.2410674/1‑150 (MQ=60)
                AAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATG                                                                                                                                  <  SRR19973070.887198/150‑1 (MQ=60)
                AAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATG                                                                                                                                  <  SRR19973070.874455/150‑1 (MQ=60)
                AAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATG                                                                                                                                  <  SRR19973070.758602/150‑1 (MQ=60)
                   CAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAG                                                                                                                               >  SRR19973070.1437499/1‑150 (MQ=60)
                   CAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGAGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAG                                                                                                                               <  SRR19973070.990894/150‑1 (MQ=60)
                    AATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGT                                                                                                                              >  SRR19973070.3012039/1‑150 (MQ=60)
                     ATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTT                                                                                                                             <  SRR19973070.2714919/150‑1 (MQ=60)
                     ATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTT                                                                                                                             <  SRR19973070.2714817/150‑1 (MQ=60)
                       AGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCG                                                                                                                           <  SRR19973070.655568/150‑1 (MQ=60)
                       AGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCG                                                                                                                           <  SRR19973070.2203366/150‑1 (MQ=60)
                       AGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCG                                                                                                                           <  SRR19973070.2203347/150‑1 (MQ=60)
                       AGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCG                                                                                                                           <  SRR19973070.2553043/150‑1 (MQ=60)
                       AGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCG                                                                                                                           <  SRR19973070.2553138/150‑1 (MQ=60)
                          TACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGT                                                                                                                        <  SRR19973070.1710276/150‑1 (MQ=60)
                          TACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGT                                                                                                                        >  SRR19973070.2178376/1‑150 (MQ=60)
                          TACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGT                                                                                                                        >  SRR19973070.2178318/1‑150 (MQ=60)
                          TACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGT                                                                                                                        >  SRR19973070.1281269/1‑150 (MQ=60)
                           ACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTG                                                                                                                       <  SRR19973070.1526587/150‑1 (MQ=60)
                            CAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGC                                                                                                                      >  SRR19973070.2077617/1‑150 (MQ=60)
                             AATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCT                                                                                                                     <  SRR19973070.2214290/150‑1 (MQ=60)
                              ATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTT                                                                                                                    >  SRR19973070.2280015/1‑150 (MQ=60)
                              ATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGCCGGTGGAGATAAGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTATTATGCAGTTCGGGagatt                                                                                                                    >  SRR19973070.607292/1‑145 (MQ=60)
                               TGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATTACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTT                                                                                                                   <  SRR19973070.2504783/150‑1 (MQ=60)
                                GCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTG                                                                                                                  <  SRR19973070.165454/150‑1 (MQ=60)
                                  GAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTG                                                                                                                <  SRR19973070.1118463/150‑1 (MQ=60)
                                  GAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTG                                                                                                                <  SRR19973070.2074934/150‑1 (MQ=60)
                                    GATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGAT                                                                                                              <  SRR19973070.2116039/150‑1 (MQ=60)
                                           AGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCC                                                                                                       >  SRR19973070.1424204/1‑150 (MQ=60)
                                             CTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAANGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCAT                                                                                                     >  SRR19973070.1691437/1‑150 (MQ=60)
                                             CTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAANGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCAT                                                                                                     >  SRR19973070.1690578/1‑150 (MQ=60)
                                                     GCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAA                                                                                             <  SRR19973070.1131444/150‑1 (MQ=60)
                                                        AGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTG                                                                                          >  SRR19973070.1069790/1‑150 (MQ=60)
                                                            GAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCG                                                                                      <  SRR19973070.2454395/150‑1 (MQ=60)
                                                              GTCATGGGCGAATACCTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTG                                                                                    <  SRR19973070.2046267/150‑1 (MQ=60)
                                                              GTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTG                                                                                    <  SRR19973070.2948903/150‑1 (MQ=60)
                                                              GTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTG                                                                                    <  SRR19973070.2044289/150‑1 (MQ=60)
                                                              GTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTG                                                                                    <  SRR19973070.2044208/150‑1 (MQ=60)
                                                                   GGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCAT                                                                               <  SRR19973070.765188/150‑1 (MQ=60)
                                                                       GAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCAC                                                                           >  SRR19973070.1423909/1‑150 (MQ=60)
                                                                       GAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCAC                                                                           >  SRR19973070.2098737/1‑150 (MQ=60)
                                                                                      GGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAAC                                                            >  SRR19973070.3029932/1‑150 (MQ=60)
                                                                                      GGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAAC                                                            >  SRR19973070.895202/1‑150 (MQ=60)
                                                                                      GGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAAC                                                            >  SRR19973070.2782393/1‑150 (MQ=60)
                                                                                        ACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAT                                                          >  SRR19973070.1733398/1‑150 (MQ=60)
                                                                                        ACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAT                                                          <  SRR19973070.2616981/150‑1 (MQ=60)
                                                                                        ACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAT                                                          <  SRR19973070.949074/150‑1 (MQ=60)
                                                                                         CCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATG                                                         <  SRR19973070.741457/150‑1 (MQ=60)
                                                                                              CGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTA                                                    <  SRR19973070.396126/150‑1 (MQ=60)
                                                                                               GGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAG                                                   >  SRR19973070.1681933/1‑150 (MQ=60)
                                                                                                   GAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAA                                               >  SRR19973070.1941346/1‑150 (MQ=60)
                                                                                                    AGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAAT                                              <  SRR19973070.1861395/150‑1 (MQ=60)
                                                                                                     GATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATA                                             <  SRR19973070.3277756/150‑1 (MQ=60)
                                                                                                     GATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATA                                             >  SRR19973070.2982600/1‑150 (MQ=60)
                                                                                                      ATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAA                                            >  SRR19973070.212524/1‑150 (MQ=60)
                                                                                                          GACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGC                                        >  SRR19973070.2660765/1‑150 (MQ=60)
                                                                                                             ACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAATCAAACATGCCGTAGACAATAATTGCGCT                                     <  SRR19973070.697703/150‑1 (MQ=60)
                                                                                                                CCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAG                                  <  SRR19973070.714425/150‑1 (MQ=60)
                                                                                                                     GAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAAT                             >  SRR19973070.2489083/1‑150 (MQ=60)
                                                                                                                     GAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAAT                             <  SRR19973070.1383307/150‑1 (MQ=60)
                                                                                                                      AACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATG                            >  SRR19973070.183660/1‑150 (MQ=60)
                                                                                                                           GAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGTTGCTTTGTGATACCAGCCATCGTAATAAATGAGCGTTACAATCCACAGAACTAAAGCAAACATTCCGTAGACAATAATTGCGCTTAGCCAATGGAAGG                       >  SRR19973070.2998206/1‑150 (MQ=60)
                                                                                                                           GAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGG                       <  SRR19973070.2791130/150‑1 (MQ=60)
                                                                                                                           GAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGG                       >  SRR19973070.2537972/1‑150 (MQ=60)
                                                                                                                             ATAACCAGCCCCATCATTAAAAAAATACCGATGGTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCC                     >  SRR19973070.1468328/1‑150 (MQ=60)
                                                                                                                             ATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCC                     <  SRR19973070.2963412/150‑1 (MQ=60)
                                                                                                                             ATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCC                     >  SRR19973070.1468172/1‑150 (MQ=60)
                                                                                                                             ATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCC                     <  SRR19973070.512341/150‑1 (MQ=60)
                                                                                                                             ATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCC                     <  SRR19973070.3091247/150‑1 (MQ=60)
                                                                                                                             ATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCC                     <  SRR19973070.2962690/150‑1 (MQ=60)
                                                                                                                                   AGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGAT               >  SRR19973070.1765690/1‑150 (MQ=60)
                                                                                                                                    GCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATA              <  SRR19973070.828595/150‑1 (MQ=60)
                                                                                                                                       CCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAA           <  SRR19973070.86432/150‑1 (MQ=60)
                                                                                                                                        CATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAAC          <  SRR19973070.2002161/150‑1 (MQ=60)
                                                                                                                                        CATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAAC          <  SRR19973070.1888508/150‑1 (MQ=60)
                                                                                                                                         ATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACA         <  SRR19973070.2445906/150‑1 (MQ=60)
                                                                                                                                          TCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACAC        <  SRR19973070.2119728/150‑1 (MQ=60)
                                                                                                                                           CATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACAAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAAAAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACC       >  SRR19973070.3183000/1‑150 (MQ=60)
                                                                                                                                             TTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCAT     <  SRR19973070.1019824/150‑1 (MQ=60)
                                                                                                                                              TAAAAAAATACCGATGCTTTTATGCAGTTAGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATA    >  SRR19973070.2538652/1‑150 (MQ=60)
                                                                                                                                                AAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGC  <  SRR19973070.173116/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                    
TGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGC  >  CP009273/1114184‑1114477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑