Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,114,329 C→A 100% L58F (TTG→TTT)  yceJ ← putative cytochrome b561

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,114,3290CA98.7% 767.4 / 0.0 234L58F (TTG→TTT) yceJputative cytochrome b561
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (95/136);  total (96/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 244 total aligned reads displayed.

CTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGCG  >  CP009273/1114183‑1114478
                                                                                                                                                  |                                                                                                                                                     
cTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTaaaaaa                                                                                                                                                    <  1:3514150/150‑1 (MQ=255)
cTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTaaaaaa                                                                                                                                                    >  1:758949/1‑150 (MQ=255)
  gATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAAt                                                                                                                                                  >  1:3140902/1‑150 (MQ=255)
   aTGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATa                                                                                                                                                 <  2:1327954/150‑1 (MQ=255)
   aTGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATa                                                                                                                                                 >  1:4501972/1‑150 (MQ=255)
   aTGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATa                                                                                                                                                 >  1:3203120/1‑150 (MQ=255)
    tGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATAc                                                                                                                                                >  2:80761/1‑150 (MQ=255)
     gCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATAcc                                                                                                                                               <  2:2351109/150‑1 (MQ=255)
      ccGATGGCGAATAGCAATAGGTACCATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCg                                                                                                                                              <  2:202992/150‑1 (MQ=255)
      ccGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCg                                                                                                                                              <  1:4244652/150‑1 (MQ=255)
        gATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGAt                                                                                                                                            >  2:197735/1‑150 (MQ=255)
        gATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGAt                                                                                                                                            <  1:1933614/150‑1 (MQ=255)
        gATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGAt                                                                                                                                            >  2:714023/1‑150 (MQ=255)
        gATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGAt                                                                                                                                            <  1:4044142/150‑1 (MQ=255)
            gCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCtt                                                                                                                                        <  2:3744542/150‑1 (MQ=255)
              gAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCtttt                                                                                                                                      >  2:4902795/1‑150 (MQ=255)
              gAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACACGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCtttt                                                                                                                                      <  2:2754521/150‑1 (MQ=255)
               aaaaGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTa                                                                                                                                     <  1:1874562/150‑1 (MQ=255)
                 aaGCAAGAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATg                                                                                                                                   >  2:1702860/1‑150 (MQ=255)
                    cAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAg                                                                                                                                >  1:651317/1‑150 (MQ=255)
                    cAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAg                                                                                                                                >  1:3655497/1‑150 (MQ=255)
                      aTAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGtt                                                                                                                              <  1:1365191/150‑1 (MQ=255)
                       tAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTc                                                                                                                             >  2:455825/1‑150 (MQ=255)
                        aGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCg                                                                                                                            <  1:4209441/150‑1 (MQ=255)
                           tACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGt                                                                                                                         >  1:4672196/1‑150 (MQ=255)
                            aCAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTg                                                                                                                        <  1:2790805/150‑1 (MQ=255)
                            aCAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTg                                                                                                                        >  1:4128815/1‑150 (MQ=255)
                            aCAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTg                                                                                                                        >  1:1803441/1‑150 (MQ=255)
                                tGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCttt                                                                                                                    <  2:4434857/150‑1 (MQ=255)
                                  cGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTtgt                                                                                                                  <  1:3173180/150‑1 (MQ=255)
                                   gagaTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTtgtg                                                                                                                 <  2:4082136/150‑1 (MQ=255)
                                      aTGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATa                                                                                                              >  1:2645675/1‑150 (MQ=255)
                                       tGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATAc                                                                                                             >  1:1969159/1‑150 (MQ=255)
                                       tGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATAc                                                                                                             <  2:2384540/150‑1 (MQ=255)
                                         tCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCa                                                                                                           >  2:1152933/1‑150 (MQ=255)
                                            aGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGcc                                                                                                        <  2:2889166/150‑1 (MQ=255)
                                            aGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGcc                                                                                                        <  2:1792023/150‑1 (MQ=255)
                                             gCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCa                                                                                                       <  1:4784339/150‑1 (MQ=255)
                                             gCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCa                                                                                                       <  1:4744136/150‑1 (MQ=255)
                                                ctTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCg                                                                                                    <  1:1702860/150‑1 (MQ=255)
                                                  tGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGta                                                                                                  >  1:1009716/1‑150 (MQ=255)
                                                  tGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGta                                                                                                  >  2:2423056/1‑150 (MQ=255)
                                                  tGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGta                                                                                                  >  2:634591/1‑150 (MQ=255)
                                                    cGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGtaat                                                                                                >  2:539004/1‑150 (MQ=255)
                                                     ggCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGtaata                                                                                               >  2:4643790/1‑150 (MQ=255)
                                                        aaGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATTGTAATAACt                                                                                            >  2:3894849/1‑150 (MQ=255)
                                                        aaGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACt                                                                                            >  2:2261796/1‑150 (MQ=255)
                                                        aaGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACt                                                                                            >  1:2348371/1‑150 (MQ=255)
                                                              aGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGt                                                                                      <  1:4445934/150‑1 (MQ=255)
                                                                 cATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGAc                                                                                   <  2:1811520/150‑1 (MQ=255)
                                                                  aTGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGAcc                                                                                  <  1:2450192/150‑1 (MQ=255)
                                                                       cGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCca                                                                             <  1:3451466/150‑1 (MQ=255)
                                                                       cGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCca                                                                             <  2:3964205/150‑1 (MQ=255)
                                                                        gAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCcac                                                                            <  1:3947156/150‑1 (MQ=255)
                                                                         aaTAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAAc                                                                                             <  1:1916777/132‑1 (MQ=255)
                                                                         aaTAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCcaca                                                                           >  1:3842627/1‑150 (MQ=255)
                                                                         aaTAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCcaca                                                                           >  2:3390178/1‑150 (MQ=255)
                                                                              cTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCt                                                                      <  1:52295/150‑1 (MQ=255)
                                                                              cTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCt                                                                      <  2:3560173/150‑1 (MQ=255)
                                                                               tCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTa                                                                     <  2:2043083/150‑1 (MQ=255)
                                                                                   cAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGc                                                                 <  1:4153285/150‑1 (MQ=255)
                                                                                     gCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCaa                                                               <  1:386560/150‑1 (MQ=255)
                                                                                      cGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCaaa                                                              >  1:221600/1‑150 (MQ=255)
                                                                                       ggACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAAc                                                             <  1:1509936/150‑1 (MQ=255)
                                                                                        gACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACa                                                            <  1:4348665/150‑1 (MQ=255)
                                                                                         aCCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAt                                                           <  2:18783/150‑1 (MQ=255)
                                                                                         aCCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAt                                                           >  1:451597/1‑150 (MQ=255)
                                                                                         aCCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAt                                                           >  1:7142/1‑150 (MQ=255)
                                                                                         aCCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAt                                                           <  1:1640816/150‑1 (MQ=255)
                                                                                           ccGGCGGGGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGc                                                         <  2:1988542/150‑1 (MQ=255)
                                                                                             ggcggTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCg                                                       >  2:4403160/1‑150 (MQ=255)
                                                                                               cggTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTa                                                     >  2:846302/1‑150 (MQ=255)
                                                                                               aggTTGAGATATGACACGCCACAGAACGAGAATAACCAGCACCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATAGTAATAACTGAGCGTGACCATCCACAGACCTTAAGCAAACATGCCGTa                                                     <  2:3027920/149‑1 (MQ=255)
                                                                                                ggTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAg                                                    >  2:4816674/1‑150 (MQ=255)
                                                                                                         atGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAAtt                                           >  1:1946183/1‑150 (MQ=255)
                                                                                                               cGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCtt                                     >  1:2502789/1‑150 (MQ=255)
                                                                                                               cGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCtt                                     <  2:3655497/150‑1 (MQ=255)
                                                                                                                 ccACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAg                                   >  2:3464009/1‑150 (MQ=255)
                                                                                                                 ccACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAg                                   <  1:2596371/150‑1 (MQ=255)
                                                                                                                   acaGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGcc                                 >  1:181809/1‑150 (MQ=255)
                                                                                                                    caGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCa                                >  2:2197654/1‑150 (MQ=255)
                                                                                                                      gAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAAt                              <  1:391825/150‑1 (MQ=255)
                                                                                                                         cgcgAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATAGCGCTTAGCCAATGGa                           >  2:1129889/1‑150 (MQ=255)
                                                                                                                           cgAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAg                         >  1:3963347/1‑150 (MQ=255)
                                                                                                                            gAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAgg                        <  1:2768990/150‑1 (MQ=255)
                                                                                                                            gAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCACCCTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAgg                        >  2:1463874/1‑150 (MQ=255)
                                                                                                                              aTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGcc                      <  1:3616623/150‑1 (MQ=255)
                                                                                                                              aTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGcc                      <  1:1129845/150‑1 (MQ=255)
                                                                                                                              aTAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGcc                      <  1:4902795/150‑1 (MQ=255)
                                                                                                                                 aCCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCa                   <  2:4535045/150‑1 (MQ=255)
                                                                                                                                 aCCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCa                   <  2:1581043/150‑1 (MQ=255)
                                                                                                                                    aGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGat                <  2:5067181/150‑1 (MQ=255)
                                                                                                                                     gCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCTTCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGata               <  1:2042995/150‑1 (MQ=255)
                                                                                                                                     gCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGata               <  1:608378/150‑1 (MQ=255)
                                                                                                                                      ccccATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGatat              <  2:3652045/150‑1 (MQ=255)
                                                                                                                                       cccATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGAtata             <  1:2646879/150‑1 (MQ=255)
                                                                                                                                       cccATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGAtata             <  1:1484090/150‑1 (MQ=255)
                                                                                                                                          atcatTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAaca          <  2:3140902/150‑1 (MQ=255)
                                                                                                                                               tAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATa     <  1:1733154/150‑1 (MQ=255)
                                                                                                                                                  cAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGCg  >  2:3447257/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGCG  >  CP009273/1114183‑1114478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGCG  >  CP009273/1114180‑1114478
                                                                                                                                                     |                                                                                                                                                     
CCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAA                                                                                                                                                       >  SRR19973063.1185578/1‑150 (MQ=60)
 CGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAA                                                                                                                                                      <  SRR19973063.2663594/150‑1 (MQ=60)
 CGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAA                                                                                                                                                      <  SRR19973063.2663545/150‑1 (MQ=60)
   CTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGTAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAA                                                                                                                                                    >  SRR19973063.760645/1‑150 (MQ=60)
   CTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAA                                                                                                                                                    >  SRR19973063.761161/1‑150 (MQ=60)
   CTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAA                                                                                                                                                    <  SRR19973063.3525389/150‑1 (MQ=60)
   CTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAA                                                                                                                                                    <  SRR19973063.3525345/150‑1 (MQ=60)
    TGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGAAACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAA                                                                                                                                                   >  SRR19973063.199394/1‑150 (MQ=60)
     GATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAAT                                                                                                                                                  >  SRR19973063.785442/1‑150 (MQ=60)
     GATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAAT                                                                                                                                                  >  SRR19973063.3151004/1‑150 (MQ=60)
     GATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAAT                                                                                                                                                  >  SRR19973063.1716712/1‑150 (MQ=60)
      ATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATA                                                                                                                                                 >  SRR19973063.3213370/1‑150 (MQ=60)
      ATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATA                                                                                                                                                 >  SRR19973063.4516425/1‑150 (MQ=60)
      ATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATA                                                                                                                                                 >  SRR19973063.3213423/1‑150 (MQ=60)
         CCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCG                                                                                                                                              <  SRR19973063.4258181/150‑1 (MQ=60)
         CCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCG                                                                                                                                              <  SRR19973063.4254710/150‑1 (MQ=60)
           GATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGAT                                                                                                                                            <  SRR19973063.4057100/150‑1 (MQ=60)
           GATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGAT                                                                                                                                            <  SRR19973063.1939658/150‑1 (MQ=60)
           GATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGAT                                                                                                                                            <  SRR19973063.1939653/150‑1 (MQ=60)
              GGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACTCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCT                                                                                                                                         >  SRR19973063.1392867/1‑150 (MQ=60)
                CGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTT                                                                                                                                       >  SRR19973063.2222064/1‑150 (MQ=60)
                  AAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTA                                                                                                                                     >  SRR19973063.1797568/1‑150 (MQ=60)
                  AAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTA                                                                                                                                     <  SRR19973063.1880422/150‑1 (MQ=60)
                       CAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAG                                                                                                                                >  SRR19973063.653227/1‑150 (MQ=60)
                       CAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAG                                                                                                                                >  SRR19973063.3667219/1‑150 (MQ=60)
                         ATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTT                                                                                                                              <  SRR19973063.1369304/150‑1 (MQ=60)
                           AGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCG                                                                                                                            <  SRR19973063.4222618/150‑1 (MQ=60)
                           AGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCG                                                                                                                            <  SRR19973063.4222869/150‑1 (MQ=60)
                              TACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGT                                                                                                                         >  SRR19973063.4687099/1‑150 (MQ=60)
                               ACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATGTGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTG                                                                                                                        <  SRR19973063.3089743/150‑1 (MQ=60)
                               ACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTG                                                                                                                        >  SRR19973063.4142031/1‑150 (MQ=60)
                               ACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTG                                                                                                                        <  SRR19973063.2799785/150‑1 (MQ=60)
                               ACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTG                                                                                                                        >  SRR19973063.4509946/1‑150 (MQ=60)
                               ACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTG                                                                                                                        >  SRR19973063.1809126/1‑150 (MQ=60)
                               ACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTG                                                                                                                        >  SRR19973063.1809034/1‑150 (MQ=60)
                               ACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTG                                                                                                                        <  SRR19973063.1720158/150‑1 (MQ=60)
                                 AATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCT                                                                                                                      <  SRR19973063.1420796/150‑1 (MQ=60)
                                     CGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGT                                                                                                                  <  SRR19973063.457948/150‑1 (MQ=60)
                                     CGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGT                                                                                                                  <  SRR19973063.3183394/150‑1 (MQ=60)
                                         ATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATA                                                                                                              >  SRR19973063.2654133/1‑150 (MQ=60)
                                          TGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATAC                                                                                                             >  SRR19973063.1975317/1‑150 (MQ=60)
                                            TCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCA                                                                                                           >  SRR19973063.4283877/1‑150 (MQ=60)
                                                GCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCA                                                                                                       <  SRR19973063.4759241/150‑1 (MQ=60)
                                                GCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCA                                                                                                       <  SRR19973063.4799622/150‑1 (MQ=60)
                                                   CTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCG                                                                                                    <  SRR19973063.1708050/150‑1 (MQ=60)
                                                    TTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGT                                                                                                   <  SRR19973063.541033/150‑1 (MQ=60)
                                                     TGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTA                                                                                                  >  SRR19973063.1012755/1‑150 (MQ=60)
                                                     TGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTA                                                                                                  >  SRR19973063.1143020/1‑150 (MQ=60)
                                                     TGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTA                                                                                                  >  SRR19973063.1012761/1‑150 (MQ=60)
                                                       CGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAAT                                                                                                <  SRR19973063.2512803/150‑1 (MQ=60)
                                                          cgtgtgctcttccgatctAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAAC                                                                                             <  SRR19973063.1922774/132‑1 (MQ=60)
                                                          CAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAAC                                                                                             <  SRR19973063.3805223/150‑1 (MQ=60)
                                                           AAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACT                                                                                            >  SRR19973063.2355885/1‑150 (MQ=60)
                                                              ACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAG                                                                                         >  SRR19973063.4256739/1‑150 (MQ=60)
                                                              ACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAG                                                                                         <  SRR19973063.1947812/150‑1 (MQ=60)
                                                              ACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAG                                                                                         >  SRR19973063.4976448/1‑150 (MQ=60)
                                                                 AGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGT                                                                                      <  SRR19973063.4460193/150‑1 (MQ=60)
                                                                 AGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGT                                                                                      <  SRR19973063.4547269/150‑1 (MQ=60)
                                                                 AGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGT                                                                                      >  SRR19973063.1833694/1‑150 (MQ=60)
                                                                 AGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGAGCTTTGTGATACCAGCCATCGTAATAACTGAGCGT                                                                                      >  SRR19973063.1833666/1‑150 (MQ=60)
                                                                  GTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTG                                                                                     >  SRR19973063.156950/1‑150 (MQ=60)
                                                                   TCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATTCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGA                                                                                    <  SRR19973063.2288621/150‑1 (MQ=60)
                                                                     ATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACC                                                                                  <  SRR19973063.2760709/150‑1 (MQ=60)
                                                                     ATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACC                                                                                  <  SRR19973063.2458019/150‑1 (MQ=60)
                                                                     ATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGCAATAACTGAGCGTGACC                                                                                  <  SRR19973063.293366/150‑1 (MQ=60)
                                                                         GCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGAAATAACagagctataccatcc                                                                              >  SRR19973063.416314/1‑135 (MQ=60)
                                                                         GCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACACCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCC                                                                              <  SRR19973063.4145326/150‑1 (MQ=60)
                                                                          CGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCA                                                                             <  SRR19973063.4764523/150‑1 (MQ=60)
                                                                          CGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCA                                                                             <  SRR19973063.3462440/150‑1 (MQ=60)
                                                                           GAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCAC                                                                            <  SRR19973063.3959781/150‑1 (MQ=60)
                                                                           GAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCAC                                                                            <  SRR19973063.3959965/150‑1 (MQ=60)
                                                                            AATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACA                                                                           >  SRR19973063.3854847/1‑150 (MQ=60)
                                                                                 CTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCT                                                                      <  SRR19973063.52481/150‑1 (MQ=60)
                                                                                      CAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGC                                                                 <  SRR19973063.4166567/150‑1 (MQ=60)
                                                                                      CAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGC                                                                 >  SRR19973063.1489898/1‑150 (MQ=60)
                                                                                       AGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCA                                                                <  SRR19973063.632887/150‑1 (MQ=60)
                                                                                        GCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAA                                                               <  SRR19973063.387659/150‑1 (MQ=60)
                                                                                        GCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAA                                                               <  SRR19973063.387497/150‑1 (MQ=60)
                                                                                         CGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAA                                                              >  SRR19973063.222263/1‑150 (MQ=60)
                                                                                          GGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAAC                                                             <  SRR19973063.1514565/150‑1 (MQ=60)
                                                                                           GACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACA                                                            <  SRR19973063.2508149/150‑1 (MQ=60)
                                                                                           GACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACA                                                            <  SRR19973063.4362550/150‑1 (MQ=60)
                                                                                            ACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAT                                                           <  SRR19973063.3981425/150‑1 (MQ=60)
                                                                                            ACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAT                                                           <  SRR19973063.1645758/150‑1 (MQ=60)
                                                                                            ACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAT                                                           >  SRR19973063.7178/1‑150 (MQ=60)
                                                                                            ACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAT                                                           <  SRR19973063.1645834/150‑1 (MQ=60)
                                                                                            ACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAT                                                           >  SRR19973063.452854/1‑150 (MQ=60)
                                                                                            ACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAT                                                           >  SRR19973063.452864/1‑150 (MQ=60)
                                                                                            ACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAT                                                           <  SRR19973063.3245924/150‑1 (MQ=60)
                                                                                            ACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACAT                                                           >  SRR19973063.7054/1‑150 (MQ=60)
                                                                                              CCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGC                                                         <  SRR19973063.1969506/150‑1 (MQ=60)
                                                                                                GGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCG                                                       >  SRR19973063.44696/1‑150 (MQ=60)
                                                                                                    GTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGA                                                   >  SRR19973063.5052107/1‑150 (MQ=60)
                                                                                                     TGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGAC                                                  >  SRR19973063.2408496/1‑150 (MQ=60)
                                                                                                        AGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAAT                                               >  SRR19973063.3959267/1‑150 (MQ=60)
                                                                                                            ATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATT                                           >  SRR19973063.1952273/1‑150 (MQ=60)
                                                                                                              GACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGC                                         <  SRR19973063.1906699/150‑1 (MQ=60)
                                                                                                               ACACGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCG                                        <  SRR19973063.3419393/150‑1 (MQ=60)
                                                                                                                  CGCCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTT                                     >  SRR19973063.2510770/1‑150 (MQ=60)
                                                                                                                    CCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAG                                   <  SRR19973063.2604619/150‑1 (MQ=60)
                                                                                                                    CCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAG                                   <  SRR19973063.2499001/150‑1 (MQ=60)
                                                                                                                    CCACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAG                                   <  SRR19973063.3957931/150‑1 (MQ=60)
                                                                                                                      ACAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCC                                 >  SRR19973063.182372/1‑150 (MQ=60)
                                                                                                                       CAGAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCA                                <  SRR19973063.4020195/150‑1 (MQ=60)
                                                                                                                         GAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAAT                              <  SRR19973063.3571499/150‑1 (MQ=60)
                                                                                                                         GAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAAT                              <  SRR19973063.392939/150‑1 (MQ=60)
                                                                                                                         GAACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAAT                              <  SRR19973063.392870/150‑1 (MQ=60)
                                                                                                                          AACGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATG                             <  SRR19973063.4738133/150‑1 (MQ=60)
                                                                                                                            CGCGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGA                           <  SRR19973063.716120/150‑1 (MQ=60)
                                                                                                                              CGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAG                         <  SRR19973063.4139340/150‑1 (MQ=60)
                                                                                                                              CGAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAG                         >  SRR19973063.3976030/1‑150 (MQ=60)
                                                                                                                               GAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGG                        <  SRR19973063.2777917/150‑1 (MQ=60)
                                                                                                                               GAATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGG                        <  SRR19973063.2777972/150‑1 (MQ=60)
                                                                                                                                AATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGC                       >  SRR19973063.4769200/1‑150 (MQ=60)
                                                                                                                                 ATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCC                      >  SRR19973063.3664003/1‑150 (MQ=60)
                                                                                                                                 ATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCC                      <  SRR19973063.3628232/150‑1 (MQ=60)
                                                                                                                                 ATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCC                      <  SRR19973063.1133271/150‑1 (MQ=60)
                                                                                                                                 ATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCC                      <  SRR19973063.3529221/150‑1 (MQ=60)
                                                                                                                                 ATAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCC                      <  SRR19973063.4918625/150‑1 (MQ=60)
                                                                                                                                  TAACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCG                     <  SRR19973063.4902194/150‑1 (MQ=60)
                                                                                                                                    TCCAGCCTCATCATTAAAATAATTCCGATGCTTTTATGCAGTTCGGGTGCTTTTTGATACGAGCCATCGTATTAACTGAGCGTGACCATCCACAGACCTAAAGCAAACTTTCCGTAGTCAATATTTTCGCTTAGCAAATGGAAGGCCGCA                   <  SRR19973063.2725488/150‑1 (MQ=60)
                                                                                                                                    ACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCGGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCTCA                   >  SRR19973063.2649406/1‑150 (MQ=60)
                                                                                                                                    ACCAGCCCCATCATTAAAAAAATACCGATGCTTTTATGCGGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCA                   >  SRR19973063.2649447/1‑150 (MQ=60)
                                                                                                                                       AGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGAT                >  SRR19973063.4424019/1‑150 (MQ=60)
                                                                                                                                       AGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGAT                >  SRR19973063.1965826/1‑150 (MQ=60)
                                                                                                                                       AGCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGAT                <  SRR19973063.1937920/150‑1 (MQ=60)
                                                                                                                                        GCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCTTCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATA               <  SRR19973063.2049379/150‑1 (MQ=60)
                                                                                                                                        GCCCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATA               <  SRR19973063.610165/150‑1 (MQ=60)
                                                                                                                                          CCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATA             <  SRR19973063.1488632/150‑1 (MQ=60)
                                                                                                                                          CCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATA             <  SRR19973063.2655203/150‑1 (MQ=60)
                                                                                                                                          CCCATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATA             <  SRR19973063.2655341/150‑1 (MQ=60)
                                                                                                                                            CATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAAC           <  SRR19973063.1691211/150‑1 (MQ=60)
                                                                                                                                             ATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACA          <  SRR19973063.2173499/150‑1 (MQ=60)
                                                                                                                                             ATCATTAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACA          <  SRR19973063.377972/150‑1 (MQ=60)
                                                                                                                                                  TAAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATA     <  SRR19973063.1738433/150‑1 (MQ=60)
                                                                                                                                                   AACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAG    <  SRR19973063.3179114/150‑1 (MQ=60)
                                                                                                                                                   AAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAG    <  SRR19973063.2011546/150‑1 (MQ=60)
                                                                                                                                                   AAAAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAG    <  SRR19973063.3616661/150‑1 (MQ=60)
                                                                                                                                                     AAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGCG  >  SRR19973063.3022167/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CCGCTGATGCCGATGGCGAAAAGCAATAGGTACAATGCGAGATGTCCAGCTCTTGCGGCAAGACGAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGCG  >  CP009273/1114180‑1114478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑