Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,358,855 G→T 92.1% G123C (GGC→TGC)  puuB → gamma‑glutamylputrescine oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,358,8550GT92.1% 479.3 / 34.7 177G123C (GGC→TGC) puuBgamma‑glutamylputrescine oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (5/9);  new base T (88/75);  total (93/84)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

Only 100 of 187 total aligned reads displayed.

TGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACC  >  CP009273/1358709‑1358998
                                                                                                                                                  |                                                                                                                                               
  aaCTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCctgcg                                                                                                                                              >  2:188594/1‑148 (MQ=255)
    cTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCtgcggc                                                                                                                                            <  2:1137472/148‑1 (MQ=255)
    cTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCtgcggc                                                                                                                                            <  1:1645302/148‑1 (MQ=255)
    cTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCtgcggc                                                                                                                                            <  1:1645353/148‑1 (MQ=255)
       cTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTg                                                                                                                                         <  1:1198882/148‑1 (MQ=255)
       cTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTg                                                                                                                                         <  1:1744241/148‑1 (MQ=255)
        tACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGt                                                                                                                                        <  2:384212/148‑1 (MQ=255)
         aCAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGtt                                                                                                                                       <  1:2857645/148‑1 (MQ=255)
         aCAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGtt                                                                                                                                       <  1:3302634/148‑1 (MQ=255)
          cAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGttt                                                                                                                                      <  2:2091079/148‑1 (MQ=255)
          cAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGttt                                                                                                                                      <  2:2091017/148‑1 (MQ=255)
          cAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGttt                                                                                                                                      >  2:1546514/1‑148 (MQ=255)
           aGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTtg                                                                                                                                     >  2:1195185/1‑148 (MQ=255)
                 gACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGa                                                                                                                               <  2:1297296/148‑1 (MQ=255)
                     tCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatgaa                                                                                                                           >  1:1463539/1‑148 (MQ=255)
                      cGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatgaat                                                                                                                          >  2:594478/1‑148 (MQ=255)
                        aCGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatgaatga                                                                                                                        <  2:2677391/148‑1 (MQ=255)
                        aCGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatgaatga                                                                                                                        >  2:890881/1‑148 (MQ=255)
                         cGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGAt                                                                                                                       >  1:1545165/1‑148 (MQ=255)
                           tGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATaa                                                                                                                     <  2:1447741/148‑1 (MQ=255)
                           tGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATaa                                                                                                                     >  1:2363831/1‑148 (MQ=255)
                           tGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATaa                                                                                                                     >  2:948719/1‑148 (MQ=255)
                               cGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAg                                                                                                                 >  2:2307940/1‑148 (MQ=255)
                                 aaaaaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATACACAGCt                                                                                                               >  1:1072033/1‑148 (MQ=255)
                                 aaaaaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCt                                                                                                               <  2:762992/148‑1 (MQ=255)
                                  aaaaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTc                                                                                                              >  2:2806179/1‑148 (MQ=255)
                                   aaaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCg                                                                                                             >  1:892533/1‑148 (MQ=255)
                                       gCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCcac                                                                                                         >  2:1082364/1‑148 (MQ=255)
                                       gCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCcac                                                                                                         >  2:1590867/1‑148 (MQ=255)
                                       gCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCcac                                                                                                         >  1:135140/1‑148 (MQ=255)
                                       gCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCcac                                                                                                         >  2:3182261/1‑148 (MQ=255)
                                       gCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCcac                                                                                                         >  2:2126988/1‑148 (MQ=255)
                                         tACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCacac                                                                                                       >  2:2533546/1‑148 (MQ=255)
                                          aCGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACt                                                                                                      >  1:2246215/1‑148 (MQ=255)
                                          aCGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACt                                                                                                      >  1:2246186/1‑148 (MQ=255)
                                          aCGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACt                                                                                                      <  1:3007425/148‑1 (MQ=255)
                                             gCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGa                                                                                                   <  1:494503/148‑1 (MQ=255)
                                               aTGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAag                                                                                                 <  2:688688/148‑1 (MQ=255)
                                                 ggACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAagag                                                                                               <  2:1609175/148‑1 (MQ=255)
                                                  gACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGc                                                                                              >  1:3040825/1‑148 (MQ=255)
                                                      ccGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGaa                                                                                          <  2:1808109/148‑1 (MQ=255)
                                                      ccGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGaa                                                                                          <  1:686319/148‑1 (MQ=255)
                                                        gCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAag                                                                                        >  1:710277/1‑148 (MQ=255)
                                                         cccGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAaga                                                                                       <  1:2466398/148‑1 (MQ=255)
                                                           cGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAgaga                                                                                     <  1:3162243/148‑1 (MQ=255)
                                                           cGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAgaga                                                                                     <  1:1520532/148‑1 (MQ=255)
                                                             tATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAAc                                                                                   <  2:3217051/148‑1 (MQ=255)
                                                             tATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAAc                                                                                   >  2:497998/1‑148 (MQ=255)
                                                             tATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAAc                                                                                   <  1:2939490/148‑1 (MQ=255)
                                                              aTGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACt                                                                                  <  1:200940/148‑1 (MQ=255)
                                                                gCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTgg                                                                                <  1:2024052/148‑1 (MQ=255)
                                                                  tCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGa                                                                              <  2:144264/148‑1 (MQ=255)
                                                                  tCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGa                                                                              <  1:781390/148‑1 (MQ=255)
                                                                  tCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGa                                                                              <  1:248992/148‑1 (MQ=255)
                                                                   cGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGaa                                                                             <  2:2070926/148‑1 (MQ=255)
                                                                   cGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGaa                                                                             <  2:1339139/148‑1 (MQ=255)
                                                                      cagcaTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGc                                                                          <  1:166044/148‑1 (MQ=255)
                                                                           tgatgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACgg                                                                     >  2:3084287/1‑148 (MQ=255)
                                                                             atgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCa                                                                   >  1:2005208/1‑148 (MQ=255)
                                                                             atgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCa                                                                   >  1:294936/1‑148 (MQ=255)
                                                                                    aaGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACa                                                            >  1:2748715/1‑148 (MQ=255)
                                                                                     aGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAg                                                           <  2:339399/148‑1 (MQ=255)
                                                                                       gtggtgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCt                                                         <  1:2068690/148‑1 (MQ=255)
                                                                                           tgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGaa                                                     <  2:2971379/148‑1 (MQ=255)
                                                                                           tgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGaa                                                     <  1:3032977/148‑1 (MQ=255)
                                                                                            gagaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAt                                                    >  2:1455673/1‑148 (MQ=255)
                                                                                            gagaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAt                                                    >  2:799581/1‑148 (MQ=255)
                                                                                             agaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAtt                                                   <  2:616063/148‑1 (MQ=255)
                                                                                             agaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAtt                                                   >  1:3283157/1‑148 (MQ=255)
                                                                                             agaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAtt                                                   <  2:226349/148‑1 (MQ=255)
                                                                                              gaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTg                                                  >  2:1718495/1‑148 (MQ=255)
                                                                                               atcatcCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGc                                                 <  2:2147033/148‑1 (MQ=255)
                                                                                               atcatcCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGc                                                 >  2:1464850/1‑148 (MQ=255)
                                                                                                 catcCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTg                                               <  1:2532529/148‑1 (MQ=255)
                                                                                                  atcCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTgg                                              >  2:3122087/1‑148 (MQ=255)
                                                                                                    ccGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGac                                            >  1:3058787/1‑148 (MQ=255)
                                                                                                       cgAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgcc                                         <  2:2437737/148‑1 (MQ=255)
                                                                                                       cgAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgcc                                         <  2:2437699/148‑1 (MQ=255)
                                                                                                          aCGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaac                                      >  1:1885732/1‑148 (MQ=255)
                                                                                                           cGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaacg                                     >  1:377795/1‑148 (MQ=255)
                                                                                                             tATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaacgcc                                   >  1:733660/1‑148 (MQ=255)
                                                                                                                   aCGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGc                             >  1:3181690/1‑148 (MQ=255)
                                                                                                                     gTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCg                           >  1:2758043/1‑148 (MQ=255)
                                                                                                                       tATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTg                         >  1:772444/1‑148 (MQ=255)
                                                                                                                       tATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTg                         >  1:772364/1‑148 (MQ=255)
                                                                                                                          cAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAg                      >  2:3079374/1‑148 (MQ=255)
                                                                                                                          cAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAg                      >  2:3012290/1‑148 (MQ=255)
                                                                                                                          cAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAg                      >  2:348046/1‑148 (MQ=255)
                                                                                                                           aGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGt                     <  1:1332146/148‑1 (MQ=255)
                                                                                                                            gATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTa                    >  1:1616999/1‑148 (MQ=255)
                                                                                                                              ttGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGc                  >  1:307387/1‑148 (MQ=255)
                                                                                                                               tGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGcc                 >  1:2903294/1‑148 (MQ=255)
                                                                                                                                       aCTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCg         <  1:2126988/148‑1 (MQ=255)
                                                                                                                                       aCTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCg         <  1:1590867/148‑1 (MQ=255)
                                                                                                                                        cTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGc        <  1:630376/148‑1 (MQ=255)
                                                                                                                                         tACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCt       >  1:2407900/1‑148 (MQ=255)
                                                                                                                                         tACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCt       <  1:1195185/148‑1 (MQ=255)
                                                                                                                                            cGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCtata    >  2:1100466/1‑148 (MQ=255)
                                                                                                                                             gCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATAc   >  2:2801479/1‑148 (MQ=255)
                                                                                                                                              ccccTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCAGTCGCCGTGAAGTAGCCAGCGATCGCTATAcc  >  2:2729221/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACC  >  CP009273/1358709‑1358998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGC  >  CP009273/1358709‑1359003
                                                                                                                                                  |                                                                                                                                                    
TGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCG                                                                                                                                                   <  SRR19973070.1539068/150‑1 (MQ=60)
    CTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCTCCCGTATGCTCGGCAGAATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCT                                                                                                                                               <  SRR19973070.2497555/150‑1 (MQ=60)
    CTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCT                                                                                                                                               <  SRR19973070.1656722/150‑1 (MQ=60)
    CTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCT                                                                                                                                               <  SRR19973070.1656677/150‑1 (MQ=60)
    CTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCT                                                                                                                                               <  SRR19973070.828566/150‑1 (MQ=60)
    CTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCT                                                                                                                                               <  SRR19973070.1656671/150‑1 (MQ=60)
       CTACAGCCGCGACCTCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTT                                                                                                                                            <  SRR19973070.1207162/150‑1 (MQ=60)
       CTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTT                                                                                                                                            <  SRR19973070.1754535/150‑1 (MQ=60)
       CTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTT                                                                                                                                            >  SRR19973070.2226234/1‑150 (MQ=60)
       CTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTT                                                                                                                                            <  SRR19973070.1756333/150‑1 (MQ=60)
       CTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTT                                                                                                                                            <  SRR19973070.1207304/150‑1 (MQ=60)
         ACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTG                                                                                                                                          <  SRR19973070.2876160/150‑1 (MQ=60)
         ACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTG                                                                                                                                          <  SRR19973070.3323783/150‑1 (MQ=60)
                   CATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAA                                                                                                                                >  SRR19973070.1473765/1‑150 (MQ=60)
                       GACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGAT                                                                                                                            >  SRR19973070.1556027/1‑150 (MQ=60)
                         CGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAA                                                                                                                          >  SRR19973070.2379472/1‑150 (MQ=60)
                         CGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAA                                                                                                                          >  SRR19973070.1603876/1‑150 (MQ=60)
                         CGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAA                                                                                                                          >  SRR19973070.707541/1‑150 (MQ=60)
                         CGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTAAGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACAGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAA                                                                                                                          >  SRR19973070.1431586/1‑150 (MQ=60)
                           TGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAAC                                                                                                                        >  SRR19973070.354639/1‑150 (MQ=60)
                               CGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATACACAGCT                                                                                                                    >  SRR19973070.1079521/1‑150 (MQ=60)
                                 AAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCG                                                                                                                  >  SRR19973070.898838/1‑150 (MQ=60)
                                     AAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCAC                                                                                                              >  SRR19973070.136316/1‑150 (MQ=60)
                                        CTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACT                                                                                                           >  SRR19973070.2261043/1‑150 (MQ=60)
                                        CTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACT                                                                                                           >  SRR19973070.2261037/1‑150 (MQ=60)
                                        CTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACT                                                                                                           >  SRR19973070.2261066/1‑150 (MQ=60)
                                          ACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTG                                                                                                         <  SRR19973070.1995963/150‑1 (MQ=60)
                                          ACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTG                                                                                                         <  SRR19973070.3026633/150‑1 (MQ=60)
                                          ACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTG                                                                                                         <  SRR19973070.3026723/150‑1 (MQ=60)
                                             GCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAG                                                                                                      <  SRR19973070.497957/150‑1 (MQ=60)
                                              CATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGA                                                                                                     >  SRR19973070.3068246/1‑150 (MQ=60)
                                                TGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGC                                                                                                   >  SRR19973070.3060057/1‑150 (MQ=60)
                                                TGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGC                                                                                                   >  SRR19973070.3060157/1‑150 (MQ=60)
                                                 GGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCA                                                                                                  <  SRR19973070.680146/150‑1 (MQ=60)
                                                     ACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAA                                                                                              >  SRR19973070.779655/1‑150 (MQ=60)
                                                      CCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAG                                                                                             <  SRR19973070.691219/150‑1 (MQ=60)
                                                      CCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAG                                                                                             >  SRR19973070.715340/1‑150 (MQ=60)
                                                       CGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGA                                                                                            >  SRR19973070.2393522/1‑150 (MQ=60)
                                                         CCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGA                                                                                          <  SRR19973070.2482490/150‑1 (MQ=60)
                                                           CGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAAC                                                                                        >  SRR19973070.2031283/1‑150 (MQ=60)
                                                           CGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAAC                                                                                        <  SRR19973070.3182707/150‑1 (MQ=60)
                                                           CGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAAC                                                                                        <  SRR19973070.1531205/150‑1 (MQ=60)
                                                             TATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTG                                                                                      <  SRR19973070.2958433/150‑1 (MQ=60)
                                                             TATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTG                                                                                      <  SRR19973070.2311868/150‑1 (MQ=60)
                                                              ATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGG                                                                                     <  SRR19973070.202462/150‑1 (MQ=60)
                                                                GCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGA                                                                                   <  SRR19973070.2038164/150‑1 (MQ=60)
                                                                GCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGA                                                                                   <  SRR19973070.2037749/150‑1 (MQ=60)
                                                                  TCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAAC                                                                                 <  SRR19973070.250694/150‑1 (MQ=60)
                                                                  TCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAAC                                                                                 <  SRR19973070.250722/150‑1 (MQ=60)
                                                                  TCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAAC                                                                                 <  SRR19973070.786841/150‑1 (MQ=60)
                                                                    GGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGC                                                                               >  SRR19973070.3001757/1‑150 (MQ=60)
                                                                      CAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTA                                                                             <  SRR19973070.167400/150‑1 (MQ=60)
                                                                      CAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTA                                                                             <  SRR19973070.167349/150‑1 (MQ=60)
                                                                        GCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACG                                                                           <  SRR19973070.2693671/150‑1 (MQ=60)
                                                                           TGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCA                                                                        >  SRR19973070.2018785/1‑150 (MQ=60)
                                                                           TGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCA                                                                        >  SRR19973070.296971/1‑150 (MQ=60)
                                                                                TTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAA                                                                   <  SRR19973070.460214/150‑1 (MQ=60)
                                                                                 TCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAAC                                                                  >  SRR19973070.324026/1‑150 (MQ=60)
                                                                                  CGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACA                                                                 >  SRR19973070.973704/1‑150 (MQ=60)
                                                                                  CGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACA                                                                 >  SRR19973070.2766518/1‑150 (MQ=60)
                                                                                  CGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACA                                                                 >  SRR19973070.2766377/1‑150 (MQ=60)
                                                                                   GAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAG                                                                <  SRR19973070.3104122/150‑1 (MQ=60)
                                                                                       GTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGG                                                            <  SRR19973070.2082628/150‑1 (MQ=60)
                                                                                       GTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGG                                                            <  SRR19973070.2082645/150‑1 (MQ=60)
                                                                                           TGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATT                                                        >  SRR19973070.3304305/1‑150 (MQ=60)
                                                                                           TGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATT                                                        >  SRR19973070.3304243/1‑150 (MQ=60)
                                                                                           TGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATT                                                        <  SRR19973070.3052273/150‑1 (MQ=60)
                                                                                                 CATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGA                                                  <  SRR19973070.2549081/150‑1 (MQ=60)
                                                                                                  ATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGAC                                                 >  SRR19973070.3078346/1‑150 (MQ=60)
                                                                                                        GAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAAC                                           >  SRR19973070.1898537/1‑150 (MQ=60)
                                                                                                         AACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACG                                          >  SRR19973070.1357656/1‑150 (MQ=60)
                                                                                                         AACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACG                                          >  SRR19973070.1486522/1‑150 (MQ=60)
                                                                                                         AACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACG                                          >  SRR19973070.380562/1‑150 (MQ=60)
                                                                                                           CGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCC                                        >  SRR19973070.738855/1‑150 (MQ=60)
                                                                                                                CAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCG                                   >  SRR19973070.1256054/1‑150 (MQ=60)
                                                                                                                 AAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGC                                  >  SRR19973070.3202279/1‑150 (MQ=60)
                                                                                                                 AAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGC                                  >  SRR19973070.2834478/1‑150 (MQ=60)
                                                                                                                   ACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCG                                >  SRR19973070.2775738/1‑150 (MQ=60)
                                                                                                                     GTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTG                              >  SRR19973070.777786/1‑150 (MQ=60)
                                                                                                                     GTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTG                              >  SRR19973070.777778/1‑150 (MQ=60)
                                                                                                                     GTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTG                              >  SRR19973070.777859/1‑150 (MQ=60)
                                                                                                                     GTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTG                              >  SRR19973070.2921657/1‑150 (MQ=60)
                                                                                                                      TTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGA                             >  SRR19973070.2960062/1‑150 (MQ=60)
                                                                                                                          CAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTA                         >  SRR19973070.1628248/1‑150 (MQ=60)
                                                                                                                           AGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAG                        <  SRR19973070.1341326/150‑1 (MQ=60)
                                                                                                                            GATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCACTAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGC                       >  SRR19973070.309558/1‑150 (MQ=60)
                                                                                                                            GATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGC                       >  SRR19973070.309568/1‑150 (MQ=60)
                                                                                                                            GATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGC                       <  SRR19973070.1774831/150‑1 (MQ=60)
                                                                                                                             ATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCC                      >  SRR19973070.2922069/1‑150 (MQ=60)
                                                                                                                                       ACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCT            <  SRR19973070.1601993/150‑1 (MQ=60)
                                                                                                                                       ACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCT            <  SRR19973070.2141215/150‑1 (MQ=60)
                                                                                                                                       ACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCT            >  SRR19973070.2423778/1‑150 (MQ=60)
                                                                                                                                       ACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCT            >  SRR19973070.1647210/1‑150 (MQ=60)
                                                                                                                                        CTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTA           <  SRR19973070.634846/150‑1 (MQ=60)
                                                                                                                                        CTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTA           <  SRR19973070.633139/150‑1 (MQ=60)
                                                                                                                                        CTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTA           <  SRR19973070.763396/150‑1 (MQ=60)
                                                                                                                                         TACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTAT          <  SRR19973070.1203585/150‑1 (MQ=60)
                                                                                                                                               CCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGT    >  SRR19973070.271446/1‑150 (MQ=60)
                                                                                                                                                 CTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGC  >  SRR19973070.3262419/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
TGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTGC  >  CP009273/1358709‑1359003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑