Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,358,855 G→T 100% G123C (GGC→TGC)  puuB → gamma‑glutamylputrescine oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,358,8550GT98.7% 744.8 / 0.7 233G123C (GGC→TGC) puuBgamma‑glutamylputrescine oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (104/126);  total (105/128)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 238 total aligned reads displayed.

AACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCG  >  CP009273/1358711‑1358999
                                                                                                                                                |                                                                                                                                                
aaCTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCctgcg                                                                                                                                               >  1:2921793/1‑148 (MQ=255)
  cTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCtgcggc                                                                                                                                             >  2:340219/1‑148 (MQ=255)
     cTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTg                                                                                                                                          >  1:3301680/1‑148 (MQ=255)
      tACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGt                                                                                                                                         >  2:1167574/1‑148 (MQ=255)
      tACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGt                                                                                                                                         <  2:237381/148‑1 (MQ=255)
         aGCCGCGACAGAGACGTGATCGAAACAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGATATCATCCGAGAACGTATCAAACGTTATAAGTTTGAATGCGACTACCGCCCCTGCGGCCTGTTtg                                                                                                                                      <  2:3473116/148‑1 (MQ=255)
          gCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTtgt                                                                                                                                     <  1:1386024/148‑1 (MQ=255)
          gCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTtgt                                                                                                                                     <  2:2294104/148‑1 (MQ=255)
           ccGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTtgtg                                                                                                                                    >  2:2752630/1‑148 (MQ=255)
             gcgACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGc                                                                                                                                  >  1:2236724/1‑148 (MQ=255)
                  aTCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatga                                                                                                                             <  1:516409/148‑1 (MQ=255)
                  aTCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatga                                                                                                                             <  1:3123886/148‑1 (MQ=255)
                    cGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGatgaat                                                                                                                           <  2:2285596/148‑1 (MQ=255)
                       cGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGAt                                                                                                                        >  1:3197997/1‑148 (MQ=255)
                       cGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGAt                                                                                                                        <  1:459756/148‑1 (MQ=255)
                       cGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGAt                                                                                                                        >  1:1636867/1‑148 (MQ=255)
                        gTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATa                                                                                                                       <  2:365617/148‑1 (MQ=255)
                          gATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATaaa                                                                                                                     >  2:816923/1‑148 (MQ=255)
                           aTCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAAc                                                                                                                    <  2:309864/148‑1 (MQ=255)
                             cGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAg                                                                                                                  <  2:2782081/148‑1 (MQ=255)
                               aaaaaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCt                                                                                                                >  2:415689/1‑148 (MQ=255)
                                 aaaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCg                                                                                                              >  2:3489563/1‑148 (MQ=255)
                                  aaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGc                                                                                                             >  2:1369322/1‑148 (MQ=255)
                                  aaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGc                                                                                                             <  1:893425/148‑1 (MQ=255)
                                  aaaGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGc                                                                                                             <  1:1806362/148‑1 (MQ=255)
                                     gCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCcac                                                                                                          >  1:875008/1‑148 (MQ=255)
                                     gCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCcac                                                                                                          >  1:256439/1‑148 (MQ=255)
                                       tACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCacac                                                                                                        >  1:78623/1‑148 (MQ=255)
                                        aCGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACt                                                                                                       >  1:2735508/1‑148 (MQ=255)
                                           gCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGa                                                                                                    >  1:2162512/1‑148 (MQ=255)
                                            cATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAATCGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGaa                                                                                                   >  2:2080869/1‑148 (MQ=255)
                                            cATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGAGGAATGATAAACAGCTCGCCACACTTGaa                                                                                                   >  1:2911069/1‑148 (MQ=255)
                                             aTGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAag                                                                                                  <  2:350305/148‑1 (MQ=255)
                                              tGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAaga                                                                                                 <  2:3161197/148‑1 (MQ=255)
                                                gACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGc                                                                                               <  1:2686355/148‑1 (MQ=255)
                                                gACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGc                                                                                               <  1:2685714/148‑1 (MQ=255)
                                                 acacCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCa                                                                                              >  2:2647795/1‑148 (MQ=255)
                                                  cacCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAg                                                                                             <  2:1241913/148‑1 (MQ=255)
                                                   acCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGa                                                                                            <  2:2013528/148‑1 (MQ=255)
                                                   acCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGa                                                                                            >  1:1389389/1‑148 (MQ=255)
                                                    ccGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGaa                                                                                           <  2:2787865/148‑1 (MQ=255)
                                                       cccGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAaga                                                                                        <  2:758273/148‑1 (MQ=255)
                                                       cccGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAaga                                                                                        >  2:838020/1‑148 (MQ=255)
                                                         cGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAgaga                                                                                      <  1:2354915/148‑1 (MQ=255)
                                                             tGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGACCGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTg                                                                                  <  2:1410451/148‑1 (MQ=255)
                                                              gCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGAGAACGTATCACACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAATCAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTgg                                                                                 <  2:3480258/148‑1 (MQ=255)
                                                               cTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTggg                                                                                >  1:1226897/1‑148 (MQ=255)
                                                                tCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGa                                                                               <  1:3404827/148‑1 (MQ=255)
                                                                tCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGa                                                                               <  1:2203608/148‑1 (MQ=255)
                                                                 cGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGaa                                                                              <  1:380058/148‑1 (MQ=255)
                                                                 cGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGaa                                                                              >  1:1986481/1‑148 (MQ=255)
                                                                 cGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGaa                                                                              <  1:1942156/148‑1 (MQ=255)
                                                                  ggCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAAc                                                                             >  2:1585756/1‑148 (MQ=255)
                                                                   gcagcaTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACg                                                                            <  2:790022/148‑1 (MQ=255)
                                                                   gcagcaTGATGTTCGAAGGTGGTGAGATCATCCGCAAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACg                                                                            >  2:910619/1‑148 (MQ=255)
                                                                    cagcaTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGc                                                                           <  1:723350/148‑1 (MQ=255)
                                                                    cagcaTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGc                                                                           <  1:645733/148‑1 (MQ=255)
                                                                     agcaTGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCt                                                                          <  2:61879/148‑1 (MQ=255)
                                                                         tgatgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACgg                                                                      >  1:427612/1‑148 (MQ=255)
                                                                          gatgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGc                                                                     >  2:3372986/1‑148 (MQ=255)
                                                                          gatgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGc                                                                     >  2:3323041/1‑148 (MQ=255)
                                                                          gatgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGc                                                                     >  2:3227201/1‑148 (MQ=255)
                                                                          gatgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGc                                                                     >  2:406972/1‑148 (MQ=255)
                                                                            tgTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCaa                                                                   >  2:961868/1‑148 (MQ=255)
                                                                             gTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAAt                                                                  <  2:1502592/148‑1 (MQ=255)
                                                                             gTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAAt                                                                  >  2:2463989/1‑148 (MQ=255)
                                                                               tCGAAGGTGGTGAGATCATCCGCGAAAGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATaa                                                                <  1:1009505/148‑1 (MQ=255)
                                                                                cGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATaaa                                                               <  1:3131018/148‑1 (MQ=255)
                                                                                cGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATaaa                                                               <  2:1064770/148‑1 (MQ=255)
                                                                                   aGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAg                                                            <  2:2001953/148‑1 (MQ=255)
                                                                                   aGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAg                                                            <  2:1741372/148‑1 (MQ=255)
                                                                                         tgAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGaa                                                      >  2:2101738/1‑148 (MQ=255)
                                                                                          gagaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACCGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAt                                                     >  1:215036/1‑148 (MQ=255)
                                                                                          gagaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAt                                                     >  2:768313/1‑148 (MQ=255)
                                                                                          gagaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAt                                                     <  2:3256348/148‑1 (MQ=255)
                                                                                           agaTCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAtt                                                    >  1:3260979/1‑148 (MQ=255)
                                                                                             atcatcCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGc                                                  >  2:61446/1‑148 (MQ=255)
                                                                                             atcatcCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGc                                                  <  1:3455748/148‑1 (MQ=255)
                                                                                             atcatcCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGc                                                  <  2:1258525/148‑1 (MQ=255)
                                                                                                    gcgAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgc                                           <  2:2958242/148‑1 (MQ=255)
                                                                                                        aCGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaac                                       >  1:387033/1‑148 (MQ=255)
                                                                                                         cGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaacg                                      >  1:1249294/1‑148 (MQ=255)
                                                                                                           tATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGacgccaacgcc                                    <  1:2803922/148‑1 (MQ=255)
                                                                                                             tCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCAt                                  >  2:1919823/1‑148 (MQ=255)
                                                                                                                  cGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGcc                             >  2:3105887/1‑148 (MQ=255)
                                                                                                                  cGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGcc                             >  2:387552/1‑148 (MQ=255)
                                                                                                                      aTCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGa                         >  2:2514274/1‑148 (MQ=255)
                                                                                                                       tCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGaa                        <  1:3416836/148‑1 (MQ=255)
                                                                                                                        cAGATTGACTGCGACTCCCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAg                       <  2:643797/148‑1 (MQ=255)
                                                                                                                            ttGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGc                   >  1:1079379/1‑148 (MQ=255)
                                                                                                                             tGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGcc                  <  2:2731559/148‑1 (MQ=255)
                                                                                                                             tGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGcc                  <  1:996641/148‑1 (MQ=255)
                                                                                                                             tGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGcc                  <  2:2636662/148‑1 (MQ=255)
                                                                                                                                 tGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCg              >  2:2153614/1‑148 (MQ=255)
                                                                                                                                  gCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGa             >  1:2689984/1‑148 (MQ=255)
                                                                                                                                  gCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGa             <  1:1585756/148‑1 (MQ=255)
                                                                                                                                  gCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGa             >  2:2770033/1‑148 (MQ=255)
                                                                                                                                       tACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCt        <  2:1090286/148‑1 (MQ=255)
                                                                                                                                          cGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCtata     <  2:2895724/148‑1 (MQ=255)
                                                                                                                                             cccTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCg  >  2:305524/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCG  >  CP009273/1358711‑1358999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTG  >  CP009273/1358709‑1359002
                                                                                                                                                  |                                                                                                                                                   
TGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCG                                                                                                                                                  <  SRR19973065.1071804/150‑1 (MQ=60)
TGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCG                                                                                                                                                  >  SRR19973065.352758/1‑150 (MQ=60)
TGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCG                                                                                                                                                  >  SRR19973065.2913404/1‑150 (MQ=60)
TGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCG                                                                                                                                                  >  SRR19973065.2939553/1‑150 (MQ=60)
TGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCG                                                                                                                                                  <  SRR19973065.3307730/150‑1 (MQ=60)
     TCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTG                                                                                                                                             >  SRR19973065.3321789/1‑150 (MQ=60)
        TACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACTTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTT                                                                                                                                          >  SRR19973065.1052499/1‑150 (MQ=60)
        TACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTT                                                                                                                                          <  SRR19973065.359650/150‑1 (MQ=60)
        TACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTT                                                                                                                                          >  SRR19973065.1512309/1‑150 (MQ=60)
         ACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTG                                                                                                                                         <  SRR19973065.2369383/150‑1 (MQ=60)
            GCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGG                                                                                                                                      <  SRR19973065.1395133/150‑1 (MQ=60)
            GCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGG                                                                                                                                      <  SRR19973065.1395261/150‑1 (MQ=60)
             CCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGC                                                                                                                                     >  SRR19973065.2250945/1‑150 (MQ=60)
               GCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGA                                                                                                                                   <  SRR19973065.890861/150‑1 (MQ=60)
                 GACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATG                                                                                                                                 >  SRR19973065.343447/1‑150 (MQ=60)
                 GACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATG                                                                                                                                 >  SRR19973065.2374152/1‑150 (MQ=60)
                    ATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAAT                                                                                                                              <  SRR19973065.3142910/150‑1 (MQ=60)
                    ATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAAT                                                                                                                              <  SRR19973065.519825/150‑1 (MQ=60)
                       GACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGAT                                                                                                                           >  SRR19973065.3217271/1‑150 (MQ=60)
                       GACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGAT                                                                                                                           >  SRR19973065.3217263/1‑150 (MQ=60)
                       GACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGAT                                                                                                                           >  SRR19973065.1647858/1‑150 (MQ=60)
                         CGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAA                                                                                                                         <  SRR19973065.462409/150‑1 (MQ=60)
                         CGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAA                                                                                                                         <  SRR19973065.462928/150‑1 (MQ=60)
                         CGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAA                                                                                                                         >  SRR19973065.826599/1‑150 (MQ=60)
                          GTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAA                                                                                                                        >  SRR19973065.1646534/1‑150 (MQ=60)
                           TGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAAC                                                                                                                       <  SRR19973065.647924/150‑1 (MQ=60)
                            GATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACA                                                                                                                      <  SRR19973065.3272835/150‑1 (MQ=60)
                               CGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCT                                                                                                                   <  SRR19973065.387409/150‑1 (MQ=60)
                                  AAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGC                                                                                                                >  SRR19973065.2647555/1‑150 (MQ=60)
                                    AAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCA                                                                                                              <  SRR19973065.2008153/150‑1 (MQ=60)
                                    AAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCA                                                                                                              <  SRR19973065.899100/150‑1 (MQ=60)
                                    AAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCA                                                                                                              <  SRR19973065.1818244/150‑1 (MQ=60)
                                     AAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCAC                                                                                                             >  SRR19973065.258225/1‑150 (MQ=60)
                                     AAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCAC                                                                                                             >  SRR19973065.880600/1‑150 (MQ=60)
                                       GCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACAC                                                                                                           >  SRR19973065.78296/1‑150 (MQ=60)
                                       GCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACAC                                                                                                           >  SRR19973065.79417/1‑150 (MQ=60)
                                        CTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACT                                                                                                          >  SRR19973065.2752521/1‑150 (MQ=60)
                                           CGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGA                                                                                                       >  SRR19973065.2176410/1‑150 (MQ=60)
                                            GGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAA                                                                                                      >  SRR19973065.2928789/1‑150 (MQ=60)
                                            GGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGAGGAATGATAAACAGCTCGCCACACTTGAA                                                                                                      >  SRR19973065.2928788/1‑150 (MQ=60)
                                             GCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAG                                                                                                     <  SRR19973065.2728475/150‑1 (MQ=60)
                                                  GACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAG                                                                                                <  SRR19973065.2702375/150‑1 (MQ=60)
                                                  GACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAG                                                                                                <  SRR19973065.2702400/150‑1 (MQ=60)
                                                  GACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAG                                                                                                <  SRR19973065.2703027/150‑1 (MQ=60)
                                                   ACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGA                                                                                               >  SRR19973065.1398515/1‑150 (MQ=60)
                                                         CCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGA                                                                                         >  SRR19973065.1097471/1‑150 (MQ=60)
                                                           CGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAAC                                                                                       <  SRR19973065.2369559/150‑1 (MQ=60)
                                                              ATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGG                                                                                    >  SRR19973065.509326/1‑150 (MQ=60)
                                                               TGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGG                                                                                   >  SRR19973065.1234906/1‑150 (MQ=60)
                                                                GCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGA                                                                                  <  SRR19973065.3465914/150‑1 (MQ=60)
                                                                 CTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAA                                                                                 >  SRR19973065.1999379/1‑150 (MQ=60)
                                                                  TCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAAC                                                                                <  SRR19973065.2217710/150‑1 (MQ=60)
                                                                  TCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAAC                                                                                <  SRR19973065.3425631/150‑1 (MQ=60)
                                                                   CGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACG                                                                               <  SRR19973065.382719/150‑1 (MQ=60)
                                                                   CGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACG                                                                               <  SRR19973065.1954855/150‑1 (MQ=60)
                                                                      CAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTA                                                                            <  SRR19973065.649979/150‑1 (MQ=60)
                                                                      CAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTA                                                                            <  SRR19973065.727027/150‑1 (MQ=60)
                                                                      CAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTA                                                                            <  SRR19973065.728195/150‑1 (MQ=60)
                                                                       AGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTAC                                                                           >  SRR19973065.555882/1‑150 (MQ=60)
                                                                         CATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGG                                                                         >  SRR19973065.44981/1‑150 (MQ=60)
                                                                         CATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGG                                                                         >  SRR19973065.430584/1‑150 (MQ=60)
                                                                         CATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGG                                                                         >  SRR19973065.2207040/1‑150 (MQ=60)
                                                                             ATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAAT                                                                     <  SRR19973065.1247434/150‑1 (MQ=60)
                                                                             ATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAAT                                                                     <  SRR19973065.1248411/150‑1 (MQ=60)
                                                                                 TCGAAGGTGGTGAGATCATCCGCGAAAGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAAC                                                                 <  SRR19973065.1016199/150‑1 (MQ=60)
                                                                                  CGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACA                                                                <  SRR19973065.3150068/150‑1 (MQ=60)
                                                                                  CGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACA                                                                <  SRR19973065.3150193/150‑1 (MQ=60)
                                                                                  CGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGAGACTACCGCCCCTGCGGACTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACA                                                                <  SRR19973065.3150066/150‑1 (MQ=60)
                                                                                    AAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGC                                                              >  SRR19973065.2236173/1‑150 (MQ=60)
                                                                                    AAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGC                                                              >  SRR19973065.2236188/1‑150 (MQ=60)
                                                                                      GGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTG                                                            <  SRR19973065.2924908/150‑1 (MQ=60)
                                                                                        TGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGA                                                          >  SRR19973065.1851951/1‑150 (MQ=60)
                                                                                          GTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACCGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAT                                                        >  SRR19973065.216655/1‑150 (MQ=60)
                                                                                          GTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAT                                                        >  SRR19973065.3025132/1‑150 (MQ=60)
                                                                                          GTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAT                                                        >  SRR19973065.216688/1‑150 (MQ=60)
                                                                                          GTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAAT                                                        <  SRR19973065.2118704/150‑1 (MQ=60)
                                                                                           TGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATT                                                       >  SRR19973065.3280722/1‑150 (MQ=60)
                                                                                           TGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATT                                                       >  SRR19973065.2430737/1‑150 (MQ=60)
                                                                                           TGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATT                                                       >  SRR19973065.3280777/1‑150 (MQ=60)
                                                                                               ATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTG                                                   <  SRR19973065.3476811/150‑1 (MQ=60)
                                                                                               ATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTG                                                   >  SRR19973065.1594987/1‑150 (MQ=60)
                                                                                               ATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTG                                                   <  SRR19973065.2965258/150‑1 (MQ=60)
                                                                                                   TCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACG                                               <  SRR19973065.840323/150‑1 (MQ=60)
                                                                                                        GAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAAC                                          >  SRR19973065.389731/1‑150 (MQ=60)
                                                                                                        GAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAAC                                          >  SRR19973065.391496/1‑150 (MQ=60)
                                                                                                         AACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACG                                         >  SRR19973065.1257520/1‑150 (MQ=60)
                                                                                                         AACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACG                                         >  SRR19973065.1259179/1‑150 (MQ=60)
                                                                                                             TATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCAT                                     <  SRR19973065.2821280/150‑1 (MQ=60)
                                                                                                              ATCAAACGTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATT                                    >  SRR19973065.3422502/1‑150 (MQ=60)
                                                                                                                     GTTATCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTG                             <  SRR19973065.2450654/150‑1 (MQ=60)
                                                                                                                         TCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGT                         <  SRR19973065.3437712/150‑1 (MQ=60)
                                                                                                                         TCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGT                         <  SRR19973065.2705662/150‑1 (MQ=60)
                                                                                                                         TCAGATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGT                         <  SRR19973065.2705818/150‑1 (MQ=60)
                                                                                                                            GATTGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGC                      >  SRR19973065.1086506/1‑150 (MQ=60)
                                                                                                                               TGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAG                   <  SRR19973065.2769753/150‑1 (MQ=60)
                                                                                                                               TGACTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAG                   <  SRR19973065.1003265/150‑1 (MQ=60)
                                                                                                                                  CTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGA                >  SRR19973065.2706683/1‑150 (MQ=60)
                                                                                                                                  CTGCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGA                >  SRR19973065.939841/1‑150 (MQ=60)
                                                                                                                                    GCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATC              <  SRR19973065.342592/150‑1 (MQ=60)
                                                                                                                                    GCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATC              <  SRR19973065.1959386/150‑1 (MQ=60)
                                                                                                                                    GCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATC              <  SRR19973065.172090/150‑1 (MQ=60)
                                                                                                                                    GCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATC              <  SRR19973065.171856/150‑1 (MQ=60)
                                                                                                                                    GCGACTACCGCCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATC              <  SRR19973065.1596331/150‑1 (MQ=60)
                                                                                                                                              CCCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGG    >  SRR19973065.1526595/1‑150 (MQ=60)
                                                                                                                                               CCCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGT   >  SRR19973065.1774355/1‑150 (MQ=60)
                                                                                                                                                CCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTCTACCGGTG  >  SRR19973065.940692/1‑150 (MQ=60)
                                                                                                                                                CCTGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTG  >  SRR19973065.940698/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                   
TGAACTCCTACAGCCGCGACATCGACGTGATCGAAAAAAGCTACGGCATGGACACCGCCCGTATGCTCGGCAGCATGATGTTCGAAGGTGGTGAGATCATCCGCGAACGTATCAAACGTTATCAGATTGACTGCGACTACCGCCCCGGCGGCCTGTTTGTGGCGATGAATGATAAACAGCTCGCCACACTTGAAGAGCAGAAAGAGAACTGGGAACGCTACGGCAATAAACAGCTGGAATTGCTGGACGCCAACGCCATTCGCCGTGAAGTAGCCAGCGATCGCTATACCGGTG  >  CP009273/1358709‑1359002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑