Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,457,725 G→T 93.1% intergenic (+30/‑71) paaK → / → paaX phenylacetyl‑CoA ligase/transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,457,7250GT93.1% 490.1 / 28.1 174intergenic (+30/‑71)paaK/paaXphenylacetyl‑CoA ligase/transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer
Reads supporting (aligned to +/- strand):  ref base G (6/6);  new base T (64/98);  total (70/104)
Fisher's exact test for biased strand distribution p-value = 5.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 174 total aligned reads displayed.

TTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCT  >  CP009273/1457580‑1457868
                                                                                                                                                 |                                                                                                                                               
ttAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTcc                                                                                                                                               >  1:1450922/1‑148 (MQ=255)
ttAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTcc                                                                                                                                               >  2:266913/1‑148 (MQ=255)
  aaGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAg                                                                                                                                             <  1:2644088/148‑1 (MQ=255)
  aaGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAg                                                                                                                                             <  2:2011607/148‑1 (MQ=255)
  aaGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAg                                                                                                                                             <  1:714034/148‑1 (MQ=255)
                gATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGAt                                                                                                                               <  1:2273111/148‑1 (MQ=255)
                gATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGAt                                                                                                                               >  2:760131/1‑148 (MQ=255)
                gATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGAt                                                                                                                               <  1:2424441/148‑1 (MQ=255)
                gATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGAt                                                                                                                               <  1:2423747/148‑1 (MQ=255)
                gATCTCTACCGATGTGATGATCGTTAACTGGGGCAGTATCCCGCGTTCAGAAGGCACGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCGGGCCCTGGTGGGGTAACGCTCCAGGGCCAGAAGTCGAt                                                                                                                               <  1:2226550/148‑1 (MQ=255)
                  tctcTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATAc                                                                                                                             >  1:295883/1‑148 (MQ=255)
                  tctcTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATAc                                                                                                                             >  1:2778640/1‑148 (MQ=255)
                   ctctACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACg                                                                                                                            >  1:862592/1‑148 (MQ=255)
                     ctACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGAc                                                                                                                          <  1:1206929/148‑1 (MQ=255)
                       aCCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCt                                                                                                                        >  1:3193928/1‑148 (MQ=255)
                         cGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCtgt                                                                                                                      <  2:326151/148‑1 (MQ=255)
                          gATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCtgtg                                                                                                                     <  1:1026908/148‑1 (MQ=255)
                            tgtgATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCt                                                                                                                   <  1:321792/148‑1 (MQ=255)
                            tgtgATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCt                                                                                                                   >  2:1833849/1‑148 (MQ=255)
                             gtgATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTa                                                                                                                  <  1:1775887/148‑1 (MQ=255)
                             gtgATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTa                                                                                                                  <  2:348056/148‑1 (MQ=255)
                             gtgATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTa                                                                                                                  <  2:348057/148‑1 (MQ=255)
                               gatgatCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATg                                                                                                                >  2:1605974/1‑148 (MQ=255)
                                atgatCGTTAACTGTGGTAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGa                                                                                                               >  1:1373309/1‑148 (MQ=255)
                                atgatCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGa                                                                                                               <  2:2676779/148‑1 (MQ=255)
                                  gatCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGAtt                                                                                                             <  1:580822/148‑1 (MQ=255)
                                  gatCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGAtt                                                                                                             <  1:585587/148‑1 (MQ=255)
                                    tCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCa                                                                                                           >  2:1436738/1‑148 (MQ=255)
                                    tCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCa                                                                                                           >  1:1574015/1‑148 (MQ=255)
                                    tCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCa                                                                                                           >  2:1436726/1‑148 (MQ=255)
                                        tAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATaaa                                                                                                       >  1:649503/1‑148 (MQ=255)
                                        tAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATaaa                                                                                                       >  2:822889/1‑148 (MQ=255)
                                                gCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCacaaca                                                                                               <  2:2434061/148‑1 (MQ=255)
                                                  aGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAAt                                                                                             <  2:1433135/148‑1 (MQ=255)
                                                  aGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAAt                                                                                             <  2:3267472/148‑1 (MQ=255)
                                                      tCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATaaca                                                                                         <  2:2456565/148‑1 (MQ=255)
                                                      tCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATaaca                                                                                         >  1:3121334/1‑148 (MQ=255)
                                                        ccGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATaacaac                                                                                       >  1:2973418/1‑148 (MQ=255)
                                                             ttCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACt                                                                                  >  2:297493/1‑148 (MQ=255)
                                                               cAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGa                                                                                <  2:2330210/148‑1 (MQ=255)
                                                                 gAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAg                                                                              >  2:1059866/1‑148 (MQ=255)
                                                                  aaggcaaggcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGc                                                                             <  1:330892/148‑1 (MQ=255)
                                                                    ggcaaggcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGa                                                                           <  2:131310/148‑1 (MQ=255)
                                                                    ggcaaggcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGa                                                                           >  1:2313225/1‑148 (MQ=255)
                                                                     gcatggcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAANCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGaa                                                                          <  1:1680038/148‑1 (MQ=255)
                                                                          gcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGt                                                                     <  2:2088462/148‑1 (MQ=255)
                                                                            gtgtCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTaa                                                                   <  1:2584666/148‑1 (MQ=255)
                                                                              gtCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAAc                                                                 <  1:2752980/148‑1 (MQ=255)
                                                                              gtCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAAc                                                                 >  1:3014966/1‑148 (MQ=255)
                                                                               tCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACt                                                                >  2:3095413/1‑148 (MQ=255)
                                                                                 gggTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTg                                                              <  1:2581259/148‑1 (MQ=255)
                                                                                  ggTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAATTCTATACGACCTGTGCTATGATTCAAAAATCACAACAATAACAACATACTGAAGCGAATGAGTAAACATGa                                                             >  1:25694/1‑148 (MQ=255)
                                                                                  ggTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGa                                                             <  2:1705150/148‑1 (MQ=255)
                                                                                    tgtTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATa                                                           <  1:2289799/148‑1 (MQ=255)
                                                                                     gtTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATAc                                                          >  2:930413/1‑148 (MQ=255)
                                                                                     gtTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATAc                                                          <  2:643501/148‑1 (MQ=255)
                                                                                      tttGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt                                                         >  1:986086/1‑148 (MQ=255)
                                                                                      tttGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACt                                                         >  1:2643319/1‑148 (MQ=255)
                                                                                        tGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACttt                                                       <  1:1822175/148‑1 (MQ=255)
                                                                                           tCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTAt                                                    <  1:1425110/148‑1 (MQ=255)
                                                                                            cTGCGCAATAGTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAACTCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATc                                                   <  2:725194/148‑1 (MQ=255)
                                                                                               cgcAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCaa                                                <  1:687675/148‑1 (MQ=255)
                                                                                                gcAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAAc                                               <  2:3142613/148‑1 (MQ=255)
                                                                                                gcAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAAc                                               >  2:1875913/1‑148 (MQ=255)
                                                                                                gcAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAAc                                               >  2:1497127/1‑148 (MQ=255)
                                                                                                 cAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACa                                              <  2:1726053/148‑1 (MQ=255)
                                                                                                 cAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACa                                              <  1:685509/148‑1 (MQ=255)
                                                                                                 cAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACa                                              <  2:50672/148‑1 (MQ=255)
                                                                                                 cAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACa                                              >  1:642213/1‑148 (MQ=255)
                                                                                                  aaTATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACAt                                             <  2:1867876/148‑1 (MQ=255)
                                                                                                     atTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCt                                          >  1:1978617/1‑148 (MQ=255)
                                                                                                     atTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCt                                          >  2:2240882/1‑148 (MQ=255)
                                                                                                         ttgGTGCCTGACGTATCGTGTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTTCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaa                                      <  1:1442321/148‑1 (MQ=255)
                                                                                                         ttgGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaa                                      >  2:2790148/1‑148 (MQ=255)
                                                                                                          tgGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaaa                                     <  2:1829781/148‑1 (MQ=255)
                                                                                                          tgGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaaa                                     <  1:152114/148‑1 (MQ=255)
                                                                                                            gTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACg                                   >  2:2957999/1‑148 (MQ=255)
                                                                                                            gTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACg                                   <  2:2984370/148‑1 (MQ=255)
                                                                                                                 tGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGtt                              <  1:2926958/148‑1 (MQ=255)
                                                                                                                  gACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTc                             <  2:1450931/148‑1 (MQ=255)
                                                                                                                   aCGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTcc                            >  2:1551809/1‑148 (MQ=255)
                                                                                                                    cGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCg                           >  1:1160094/1‑148 (MQ=255)
                                                                                                                      tATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGt                         >  1:77149/1‑148 (MQ=255)
                                                                                                                      tATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGt                         >  2:2878478/1‑148 (MQ=255)
                                                                                                                         cGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAg                      <  1:1735162/148‑1 (MQ=255)
                                                                                                                         cGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAg                      >  1:1127968/1‑148 (MQ=255)
                                                                                                                          gTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGt                     <  2:1198637/148‑1 (MQ=255)
                                                                                                                           tCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTg                    >  1:2347876/1‑148 (MQ=255)
                                                                                                                           tCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTg                    <  2:2973419/148‑1 (MQ=255)
                                                                                                                            cTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTgg                   >  2:2803912/1‑148 (MQ=255)
                                                                                                                             tGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGc                  >  1:1256114/1‑148 (MQ=255)
                                                                                                                              ggCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGca                 >  2:3080404/1‑148 (MQ=255)
                                                                                                                                cccTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGcaca               >  2:1740123/1‑148 (MQ=255)
                                                                                                                                   tggtggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCt            <  2:1560953/148‑1 (MQ=255)
                                                                                                                                   tggtggGGAAAAGCGCCAGGGACAGAAGTCGATACGACCTGTGATATGATTCATAAATCAAAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATAATTTTATACAAAATGCTGTAAACCCACTTCCGGTAAGTGGAACAtat            >  2:1196777/1‑146 (MQ=255)
                                                                                                                                    ggtggGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCtt           <  1:1455377/148‑1 (MQ=255)
                                                                                                                                    ggtggGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCtt           <  1:405822/148‑1 (MQ=255)
                                                                                                                                    ggtggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCtt           <  1:2755602/148‑1 (MQ=255)
                                                                                                                                        gggTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGAt       <  1:1802612/148‑1 (MQ=255)
                                                                                                                                             aaGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATctcct  >  2:2211395/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCT  >  CP009273/1457580‑1457868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTCTCTG  >  CP009273/1457576‑1457873
                                                                                                                                                     |                                                                                                                                                    
CGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCT                                                                                                                                                      <  SRR19973070.2142459/150‑1 (MQ=60)
 GGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTC                                                                                                                                                     <  SRR19973070.2452252/150‑1 (MQ=60)
 GGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTC                                                                                                                                                     >  SRR19973070.1322843/1‑150 (MQ=60)
 GGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTC                                                                                                                                                     >  SRR19973070.1322778/1‑150 (MQ=60)
  GATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCC                                                                                                                                                    >  SRR19973070.1461014/1‑150 (MQ=60)
  GATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCC                                                                                                                                                    >  SRR19973070.1461023/1‑150 (MQ=60)
     TAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGG                                                                                                                                                 <  SRR19973070.2286728/150‑1 (MQ=60)
      AAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGG                                                                                                                                                <  SRR19973070.2661311/150‑1 (MQ=60)
      AAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGG                                                                                                                                                <  SRR19973070.719128/150‑1 (MQ=60)
        GTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCC                                                                                                                                              <  SRR19973070.607252/150‑1 (MQ=60)
             TGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAG                                                                                                                                         <  SRR19973070.657342/150‑1 (MQ=60)
                    GATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATAC                                                                                                                                  <  SRR19973070.2288209/150‑1 (MQ=60)
                    GATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATAC                                                                                                                                  <  SRR19973070.2439681/150‑1 (MQ=60)
                    GATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATAC                                                                                                                                  >  SRR19973070.2796494/1‑150 (MQ=60)
                    GATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATAC                                                                                                                                  >  SRR19973070.297928/1‑150 (MQ=60)
                    GATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATAC                                                                                                                                  <  SRR19973070.2440379/150‑1 (MQ=60)
                    GATCTCTACCGATGTGATGATCGTTAACTGGGGCAGTATCCCGCGTTCAGAAGGCACGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCGGGCCCTGGTGGGGTAACGCTCCAGGGCCAGAAGTCGATAC                                                                                                                                  <  SRR19973070.2241190/150‑1 (MQ=60)
                     ATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACG                                                                                                                                 >  SRR19973070.868592/1‑150 (MQ=60)
                         CTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCT                                                                                                                             >  SRR19973070.3214598/1‑150 (MQ=60)
                         CTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCT                                                                                                                             <  SRR19973070.1215407/150‑1 (MQ=60)
                         CTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCT                                                                                                                             >  SRR19973070.1177170/1‑150 (MQ=60)
                          TACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTG                                                                                                                            <  SRR19973070.2493587/150‑1 (MQ=60)
                             CGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGC                                                                                                                         >  SRR19973070.2781463/1‑150 (MQ=60)
                              GATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCT                                                                                                                        <  SRR19973070.1034186/150‑1 (MQ=60)
                               ATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTA                                                                                                                       <  SRR19973070.1154736/150‑1 (MQ=60)
                                TGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTAT                                                                                                                      <  SRR19973070.324133/150‑1 (MQ=60)
                                 GTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGCTCTGCGCAATATTGTGGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATG                                                                                                                     <  SRR19973070.1788278/150‑1 (MQ=60)
                                 GTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATG                                                                                                                     <  SRR19973070.1788179/150‑1 (MQ=60)
                                  TGATGATCGTTAACTGTGGTAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGA                                                                                                                    >  SRR19973070.1378145/1‑150 (MQ=60)
                                  TGATGATCGTTAACTGTGGTAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGA                                                                                                                    >  SRR19973070.1382642/1‑150 (MQ=60)
                                      GATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCA                                                                                                                >  SRR19973070.802729/1‑150 (MQ=60)
                                      GATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCA                                                                                                                <  SRR19973070.584823/150‑1 (MQ=60)
                                      GATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCA                                                                                                                >  SRR19973070.1585064/1‑150 (MQ=60)
                                      GATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCA                                                                                                                <  SRR19973070.589622/150‑1 (MQ=60)
                                       ATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCAT                                                                                                               <  SRR19973070.2215627/150‑1 (MQ=60)
                                          GTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAA                                                                                                            >  SRR19973070.654131/1‑150 (MQ=60)
                                                        TATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACA                                                                                              >  SRR19973070.3141479/1‑150 (MQ=60)
                                                        TATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACA                                                                                              <  SRR19973070.173815/150‑1 (MQ=60)
                                                        TATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACA                                                                                              >  SRR19973070.1571940/1‑150 (MQ=60)
                                                          TCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAAC                                                                                            >  SRR19973070.2992513/1‑150 (MQ=60)
                                                          TCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAAC                                                                                            >  SRR19973070.2992512/1‑150 (MQ=60)
                                                             CGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGA                                                                                         >  SRR19973070.695451/1‑150 (MQ=60)
                                                                      AAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGA                                                                                <  SRR19973070.333303/150‑1 (MQ=60)
                                                                      AAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGA                                                                                >  SRR19973070.2328600/1‑150 (MQ=60)
                                                                        GGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAAT                                                                              <  SRR19973070.199134/150‑1 (MQ=60)
                                                                         GCATGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAANCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATG                                                                             <  SRR19973070.1691546/150‑1 (MQ=60)
                                                                               CGTGGCGGGTGTTTGATCTGCGCAATATTGTTGGGGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAA                                                                       <  SRR19973070.3081060/150‑1 (MQ=60)
                                                                                GTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAAC                                                                      >  SRR19973070.3034200/1‑150 (MQ=60)
                                                                                GTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAAC                                                                      <  SRR19973070.1510823/150‑1 (MQ=60)
                                                                                GTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAAC                                                                      <  SRR19973070.2601554/150‑1 (MQ=60)
                                                                                  GTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTT                                                                    <  SRR19973070.2770661/150‑1 (MQ=60)
                                                                                    CGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAATTCTATACGACCTGTGCTATGATTCAAAAATCACAACAATAACAACATACTGAAGCGAATGAGTAAACATGA                                                                  >  SRR19973070.26047/1‑150 (MQ=60)
                                                                                    CGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGA                                                                  >  SRR19973070.26050/1‑150 (MQ=60)
                                                                                     GGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGAT                                                                 >  SRR19973070.2327020/1‑150 (MQ=60)
                                                                                     GGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGAT                                                                 <  SRR19973070.2598131/150‑1 (MQ=60)
                                                                                        TGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACT                                                              >  SRR19973070.993113/1‑150 (MQ=60)
                                                                                        TGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACT                                                              >  SRR19973070.2660540/1‑150 (MQ=60)
                                                                                        TGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACT                                                              <  SRR19973070.2305023/150‑1 (MQ=60)
                                                                                        TGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACT                                                              <  SRR19973070.2361439/150‑1 (MQ=60)
                                                                                            TGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTA                                                          <  SRR19973070.1834717/150‑1 (MQ=60)
                                                                                              ATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATC                                                        >  SRR19973070.1433773/1‑150 (MQ=60)
                                                                                              ATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATC                                                        <  SRR19973070.765473/150‑1 (MQ=60)
                                                                                               TCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCC                                                       <  SRR19973070.1434947/150‑1 (MQ=60)
                                                                                               TCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCC                                                       <  SRR19973070.1434921/150‑1 (MQ=60)
                                                                                                   CGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACA                                                   >  SRR19973070.646794/1‑150 (MQ=60)
                                                                                                   CGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACA                                                   <  SRR19973070.268679/150‑1 (MQ=60)
                                                                                                   CGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACA                                                   <  SRR19973070.692580/150‑1 (MQ=60)
                                                                                                     CAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATG                                                 <  SRR19973070.690399/150‑1 (MQ=60)
                                                                                                       ATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAATCGAATGAGTAAACTTGATACTTTTATCCAACATGCT                                               >  SRR19973070.652831/1‑150 (MQ=60)
                                                                                                       ATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCT                                               >  SRR19973070.1991993/1‑150 (MQ=60)
                                                                                                        TATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTG                                              >  SRR19973070.2912708/1‑150 (MQ=60)
                                                                                                        TATTGGTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTG                                              <  SRR19973070.2947097/150‑1 (MQ=60)
                                                                                                             TTGGTGCCTGACGTATCGTGTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTTCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAAC                                         <  SRR19973070.1452331/150‑1 (MQ=60)
                                                                                                              TGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACG                                        <  SRR19973070.153392/150‑1 (MQ=60)
                                                                                                                   CCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTT                                   <  SRR19973070.2661014/150‑1 (MQ=60)
                                                                                                                     TGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCC                                 <  SRR19973070.2945850/150‑1 (MQ=60)
                                                                                                                      GACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCG                                >  SRR19973070.2383488/1‑150 (MQ=60)
                                                                                                                      GACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCG                                >  SRR19973070.1168209/1‑150 (MQ=60)
                                                                                                                        CGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGT                              >  SRR19973070.77970/1‑150 (MQ=60)
                                                                                                                         GTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATACAACATGCTGTAAACGCTGTTCCGGTC                             >  SRR19973070.1770394/1‑150 (MQ=60)
                                                                                                                           ATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAG                           >  SRR19973070.1135737/1‑150 (MQ=60)
                                                                                                                             CGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTG                         <  SRR19973070.1747189/150‑1 (MQ=60)
                                                                                                                             CGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTG                         >  SRR19973070.2363450/1‑150 (MQ=60)
                                                                                                                             CGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGACTGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTG                         <  SRR19973070.2644302/150‑1 (MQ=60)
                                                                                                                               TCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGC                       >  SRR19973070.1264920/1‑150 (MQ=60)
                                                                                                                                  GGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACA                    >  SRR19973070.3146267/1‑150 (MQ=60)
                                                                                                                                     CCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACAGCT                 >  SRR19973070.3292076/1‑150 (MQ=60)
                                                                                                                                        GGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTG              <  SRR19973070.408801/150‑1 (MQ=60)
                                                                                                                                        GGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTG              <  SRR19973070.1465519/150‑1 (MQ=60)
                                                                                                                                        GGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTG              <  SRR19973070.408808/150‑1 (MQ=60)
                                                                                                                                        GGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTG              <  SRR19973070.2773290/150‑1 (MQ=60)
                                                                                                                                            GGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCT          <  SRR19973070.3333010/150‑1 (MQ=60)
                                                                                                                                            GGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCT          <  SRR19973070.1815058/150‑1 (MQ=60)
                                                                                                                                                    CTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTCTCTG  >  SRR19973070.740881/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTCTCTG  >  CP009273/1457576‑1457873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑