Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F218 I0 R1	1506	278.7	9275112	94.5%	8764980	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,457,725	G→T	9.5%	intergenic (+30/‑71)	paaK → / → paaX	phenylacetyl‑CoA ligase/transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,457,725	0	G	T	9.5%	949.2 / 47.2	258	intergenic (+30/‑71)	paaK/paaX	phenylacetyl‑CoA ligase/transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer
Reads supporting (aligned to +/- strand): ref base G (97/136); new base T (11/14); total (108/150)
Fisher's exact test for biased strand distribution p-value = 8.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

CGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTCTCTGT  >  CP009273/1457576‑1457874

cgtaTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAgcg                                                                                                                                                       >  1:4271548/4‑150 (MQ=255)

 ggATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAgcgc                                                                                                                                                      >  2:2274589/1‑150 (MQ=255)

 ggATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAgcgc                                                                                                                                                      >  2:4117061/1‑150 (MQ=255)

  gATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGcc                                                                                                                                                     <  2:1411735/150‑1 (MQ=255)

   aTTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCa                                                                                                                                                    >  2:390378/1‑150 (MQ=255)

     tAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAgg                                                                                                                                                  >  2:1732500/1‑150 (MQ=255)

     tAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAgg                                                                                                                                                  <  2:2511667/150‑1 (MQ=255)

      aaGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAggg                                                                                                                                                 >  1:240379/1‑150 (MQ=255)

        gTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGcc                                                                                                                                               <  2:1251256/150‑1 (MQ=255)

               gtggGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTc                                                                                                                                        <  1:2632574/150‑1 (MQ=255)

                tggGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCACTATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCg                                                                                                                                       >  2:3669617/1‑150 (MQ=255)

                tggGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCg                                                                                                                                       >  1:3944259/1‑150 (MQ=255)

                  gggATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGATGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATGGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGAt                                                                                                                                     <  1:4119885/150‑1 (MQ=255)

                   ggATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATa                                                                                                                                    >  1:868519/1‑150 (MQ=255)

                      tctcTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGa                                                                                                                                 >  2:3022541/1‑150 (MQ=255)

                       ctctACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGAc                                                                                                                                >  1:1559635/1‑150 (MQ=255)

                       ctctACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGAc                                                                                                                                >  1:4449998/1‑150 (MQ=255)

                        tctACCGATGTGATGATCGTTAACTGTGGCAGTATACCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGTTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGAcc                                                                                                                               >  1:913276/1‑150 (MQ=255)

                          tACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCtg                                                                                                                             <  2:1793834/150‑1 (MQ=255)

                            ccGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCtgtg                                                                                                                           <  2:1822865/150‑1 (MQ=255)

                             cGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGc                                                                                                                          >  2:4397723/1‑150 (MQ=255)

                             cGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGc                                                                                                                          >  2:1850390/1‑150 (MQ=255)

                               aTGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTa                                                                                                                        <  1:533897/150‑1 (MQ=255)

                                 gtgATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATg                                                                                                                      >  2:2060935/1‑150 (MQ=255)

                                 gtgATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATg                                                                                                                      <  1:3476773/150‑1 (MQ=255)

                                 gtgATGATCGTAAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGCAGTGTCGGGAGTATGATCTGCGCAATATTCTTGGTGACTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATg                                                                                                                      >  2:1434967/1‑150 (MQ=255)

                                  tgatgaTCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGa                                                                                                                     >  1:4358683/1‑150 (MQ=255)

                                    atgatCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGCTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGAtt                                                                                                                   <  2:3453666/150‑1 (MQ=255)

                                      gatCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCa                                                                                                                 <  1:213005/150‑1 (MQ=255)

                                      gatCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCGGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCa                                                                                                                 >  1:2657917/1‑150 (MQ=255)

                                        tCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATa                                                                                                               <  2:629399/150‑1 (MQ=255)

                                            tAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATc                                                                                                           <  1:3796984/150‑1 (MQ=255)

                                            tAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATc                                                                                                           >  1:1984296/1‑150 (MQ=255)

                                               cTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATcaca                                                                                                        <  1:1097554/150‑1 (MQ=255)

                                                  tgGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCacaaca                                                                                                     <  2:3695367/150‑1 (MQ=255)

                                                      aGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATaa                                                                                                 >  2:362906/1‑150 (MQ=255)

                                                      aGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATaa                                                                                                 <  1:506784/150‑1 (MQ=255)

                                                         aTCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATaacaa                                                                                              >  1:4540884/1‑150 (MQ=255)

                                                           cccGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAacaaca                                                                                            <  2:928489/150‑1 (MQ=255)

                                                           cccGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAacaaca                                                                                            <  2:2298739/150‑1 (MQ=255)

                                                            ccGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAg                                                                                           <  2:4302125/150‑1 (MQ=255)

                                                             cgcgTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGa                                                                                          >  2:2119436/1‑150 (MQ=255)

                                                             cgcgTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGa                                                                                          >  2:4445976/1‑150 (MQ=255)

                                                               cgTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACt                                                                                        <  1:4513693/150‑1 (MQ=255)

                                                                 ttCAGAAGGCAAGGCGTGTCGGGTGTTTTATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAAAAGACTGa                                                                                      <  1:2750272/150‑1 (MQ=255)

                                                                    aGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGc                                                                                   <  1:1510677/150‑1 (MQ=255)

                                                                      aaggcaaggcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGa                                                                                 <  1:1868745/150‑1 (MQ=255)

                                                                       aggcaaggcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGaa                                                                                <  2:4630438/150‑1 (MQ=255)

                                                                        ggcaaggcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAAt                                                                               <  1:4210687/150‑1 (MQ=255)

                                                                        ggcaaggcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAAt                                                                               <  1:3067832/150‑1 (MQ=255)

                                                                         gcaaggcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATg                                                                              <  2:3389665/150‑1 (MQ=255)

                                                                          caaggcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGa                                                                             >  2:4229281/1‑150 (MQ=255)

                                                                           aaggcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAg                                                                            <  1:178425/150‑1 (MQ=255)

                                                                            aggcGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGt                                                                           >  1:2915912/1‑150 (MQ=255)

                                                                                 tgtCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACt                                                                      <  1:3715351/150‑1 (MQ=255)

                                                                                  gtCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACtt                                                                     <  2:1532475/150‑1 (MQ=255)

                                                                                   tCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTg                                                                    >  1:2393258/1‑150 (MQ=255)

                                                                                      ggTGTTTGATCTGCGCAATATTGTTGGGGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATa                                                                 <  1:668184/150‑1 (MQ=255)

                                                                                         gtTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACtt                                                              <  1:1011989/150‑1 (MQ=255)

                                                                                           ttGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACtttt                                                            <  1:2782679/150‑1 (MQ=255)

                                                                                            tGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTa                                                           <  1:1151938/150‑1 (MQ=255)

                                                                                             gATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTAt                                                          <  1:3280447/150‑1 (MQ=255)

                                                                                              aTCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATc                                                         >  1:2212393/1‑150 (MQ=255)

                                                                                               tCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATTCTTTTATcc                                                        <  1:430600/150‑1 (MQ=255)

                                                                                                 tGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCaa                                                      >  1:2260877/1‑150 (MQ=255)

                                                                                                   cgcAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACa                                                    <  1:2486480/150‑1 (MQ=255)

                                                                                                    gcAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACAt                                                   >  2:4019554/1‑150 (MQ=255)

                                                                                                      aaTATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGc                                                 >  1:3735292/1‑150 (MQ=255)

                                                                                                      aaTATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATTCTTTTATCCAACATGc                                                 >  1:2560908/1‑150 (MQ=255)

                                                                                                      aaTATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATTCTTTTATCCAACATGc                                                 >  2:1175955/1‑150 (MQ=255)

                                                                                                        tatTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTg                                               >  2:381836/1‑150 (MQ=255)

                                                                                                         atTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGt                                              >  1:269805/1‑150 (MQ=255)

                                                                                                            gttgGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTaaa                                           <  2:2559413/150‑1 (MQ=255)

                                                                                                             ttgGTGCCTGCCGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAAc                                          <  1:1154285/150‑1 (MQ=255)

                                                                                                             ttgGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAAc                                          >  2:2810832/1‑150 (MQ=255)

                                                                                                               ggTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGc                                        <  1:830226/150‑1 (MQ=255)

                                                                                                                    cTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTc                                   >  1:4470736/1‑150 (MQ=255)

                                                                                                                     tGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTcc                                  >  1:1418946/1‑150 (MQ=255)

                                                                                                                       aCGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCgg                                <  2:2895311/150‑1 (MQ=255)

                                                                                                                          tATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCa                             >  2:1578020/1‑150 (MQ=255)

                                                                                                                           aTCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATTCTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAg                            >  2:3190074/1‑150 (MQ=255)

                                                                                                                           aTCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATTCTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAg                            >  2:772503/1‑150 (MQ=255)

                                                                                                                                cTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATTCTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGca                       <  1:3229984/150‑1 (MQ=255)

                                                                                                                                 tGGCCCTGGTGGGGTAAAGCTCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGcac                      <  1:390378/150‑1 (MQ=255)

                                                                                                                                     ccTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATTCTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCt                  >  1:3429115/1‑150 (MQ=255)

                                                                                                                                       tggtggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCttt                <  1:1214237/150‑1 (MQ=255)

                                                                                                                                        ggtggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTg               >  2:2438780/1‑150 (MQ=255)

                                                                                                                                        ggtggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTg               >  2:2438526/1‑150 (MQ=255)

                                                                                                                                        ggtggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTg               <  2:868519/150‑1 (MQ=255)

                                                                                                                                         gtggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATTCTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGa              <  2:3574219/150‑1 (MQ=255)

                                                                                                                                          tggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATTCTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGAt             <  1:4177626/150‑1 (MQ=255)

                                                                                                                                          tggGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGAt             >  1:1350450/1‑150 (MQ=255)

                                                                                                                                            gggTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATTCTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGAtct           <  1:3471639/150‑1 (MQ=255)

                                                                                                                                             ggTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATTCTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGAtctc          <  2:3447699/150‑1 (MQ=255)

                                                                                                                                             ggTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATTCTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGAtctc          <  1:2696625/150‑1 (MQ=255)

                                                                                                                                             ggTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGAtctc          >  1:1682471/1‑150 (MQ=255)

                                                                                                                                              gTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATTCTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATctcc         <  1:2876283/150‑1 (MQ=255)

                                                                                                                                                  aGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATTCTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGatctcatctc     >  2:3720443/1‑150 (MQ=255)

                                                                                                                                                    cgcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTCTCTg   <  1:3155989/150‑1 (MQ=255)

                                                                                                                                                     gcCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTCTCTGt  <  2:3045126/150‑1 (MQ=255)

CGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAAGGCAAGGCGTGTCGGGTGTTTGATCTGCGCAATATTGTTGGTGCCTGACGTATCGTCTGGCCCTGGTGGGGTAAAGCGCCAGGGCCAGAAGTCGATACGACCTGTGCTATGATTCATAAATCACAACAATAACAACAGACTGAAGCGAATGAGTAAACTTGATACTTTTATCCAACATGCTGTAAACGCTGTTCCGGTCAGTGGCACATCTTTGATCTCCTCTCTGT  >  CP009273/1457576‑1457874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A