Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,365,607 G→T 100% I109I (ATC→ATA)  nanT ← sialic acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,365,6070GT100.0% 527.1 / NA 164I109I (ATC→ATA) nanTsialic acid transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (87/77);  total (87/77)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 164 total aligned reads displayed.

GGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAGAG  >  CP009273/3365463‑3365751
                                                                                                                                                |                                                                                                                                                
ggCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATg                                                                                                                                               <  2:3089660/148‑1 (MQ=255)
ggCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATg                                                                                                                                               <  2:2715798/148‑1 (MQ=255)
   ccccACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAg                                                                                                                                            <  1:916923/148‑1 (MQ=255)
     ccACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGcc                                                                                                                                          <  2:163378/148‑1 (MQ=255)
      cacaGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCt                                                                                                                                         >  2:3057193/1‑148 (MQ=255)
      cacaGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCt                                                                                                                                         <  1:1437830/148‑1 (MQ=255)
      cacaGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCt                                                                                                                                         >  1:166696/1‑148 (MQ=255)
        caGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTgg                                                                                                                                       <  1:1596124/148‑1 (MQ=255)
           agaAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGgcgc                                                                                                                                    >  2:2951553/1‑148 (MQ=255)
             aaGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCaa                                                                                                                                  >  1:1013403/1‑148 (MQ=255)
             aaGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCaa                                                                                                                                  >  1:3079844/1‑148 (MQ=255)
                    aaaTCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCa                                                                                                                           <  2:3177039/148‑1 (MQ=255)
                       tCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCATACGAGCGATAAAAATGGTTATGTAGCCTGGCGCAAAGCCGCAGGc                                                                                                                        <  2:1544021/148‑1 (MQ=255)
                       tCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGATCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGc                                                                                                                        <  1:2517422/148‑1 (MQ=255)
                          aaaaaCCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAg                                                                                                                     >  2:2849702/1‑148 (MQ=255)
                            aaaCCACTGGCTTTGTTACCCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTAATCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCg                                                                                                                   >  1:609989/1‑148 (MQ=255)
                             aaCCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGt                                                                                                                  >  2:727290/1‑148 (MQ=255)
                               ccACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTcc                                                                                                                <  2:652319/148‑1 (MQ=255)
                               ccACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTcc                                                                                                                >  2:278942/1‑148 (MQ=255)
                               ccACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTcc                                                                                                                <  2:349503/148‑1 (MQ=255)
                               ccACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTcc                                                                                                                >  2:1808901/1‑148 (MQ=255)
                                  cTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCgg                                                                                                             <  1:505400/148‑1 (MQ=255)
                                    ggCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGcc                                                                                                           >  2:116964/1‑148 (MQ=255)
                                    ggCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGcc                                                                                                           >  2:2929590/1‑148 (MQ=255)
                                           ttACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaaga                                                                                                    >  1:550368/1‑148 (MQ=255)
                                           ttACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaaga                                                                                                    >  2:3101365/1‑148 (MQ=255)
                                            tACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagag                                                                                                   >  1:2718167/1‑148 (MQ=255)
                                            tACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagag                                                                                                   <  2:712588/148‑1 (MQ=255)
                                            tACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagag                                                                                                   >  1:1986736/1‑148 (MQ=255)
                                             aCGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagaga                                                                                                  <  1:2378477/148‑1 (MQ=255)
                                             aCGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagaga                                                                                                  <  2:816067/148‑1 (MQ=255)
                                             aCGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagaga                                                                                                  <  1:718616/148‑1 (MQ=255)
                                               gCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAAc                                                                                                >  1:1298202/1‑148 (MQ=255)
                                               gCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAAc                                                                                                >  2:143656/1‑148 (MQ=255)
                                                cAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACg                                                                                               >  1:2234598/1‑148 (MQ=255)
                                                 aGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGa                                                                                              >  1:1562893/1‑148 (MQ=255)
                                                 aGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGa                                                                                              >  1:881954/1‑148 (MQ=255)
                                                  gATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGAt                                                                                             >  1:1828888/1‑148 (MQ=255)
                                                  gATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGAt                                                                                             >  2:1832839/1‑148 (MQ=255)
                                                   aTGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATg                                                                                            >  2:2084585/1‑148 (MQ=255)
                                                   aTGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATg                                                                                            >  2:2085778/1‑148 (MQ=255)
                                                   aTGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATg                                                                                            >  2:2908244/1‑148 (MQ=255)
                                                   aTGATTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAACCATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATg                                                                                            <  2:2856933/148‑1 (MQ=255)
                                                    tGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGc                                                                                           >  1:1884968/1‑148 (MQ=255)
                                                     gTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCt                                                                                          >  1:3311104/1‑148 (MQ=255)
                                                      ttttGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTg                                                                                         >  2:2841350/1‑148 (MQ=255)
                                                           gCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGAc                                                                                    <  2:1872061/148‑1 (MQ=255)
                                                              aGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCAt                                                                                 >  1:3152053/1‑148 (MQ=255)
                                                                cTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTg                                                                               >  2:1372525/1‑148 (MQ=255)
                                                                cTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTg                                                                               >  2:2271319/1‑148 (MQ=255)
                                                                cTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTg                                                                               >  2:2271307/1‑148 (MQ=255)
                                                                cTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTg                                                                               <  2:1840635/148‑1 (MQ=255)
                                                                 tttCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGc                                                                              <  2:588848/148‑1 (MQ=255)
                                                                  ttCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGACGAGAACGATGCTGGTGACCATTGcc                                                                             >  2:1254251/1‑148 (MQ=255)
                                                                  ttCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGcc                                                                             >  2:1109088/1‑148 (MQ=255)
                                                                    cAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAg                                                                           >  1:1484856/1‑148 (MQ=255)
                                                                    cAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAg                                                                           <  2:2137685/148‑1 (MQ=255)
                                                                       tGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAgacg                                                                        >  2:925360/1‑148 (MQ=255)
                                                                       tGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAgacg                                                                        >  1:1981498/1‑148 (MQ=255)
                                                                       tGACATAGGTGGCGCTGGAACCGTAGTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAgacg                                                                        <  2:3186628/148‑1 (MQ=255)
                                                                           aTAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCTTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGc                                                                    <  1:1049267/148‑1 (MQ=255)
                                                                            tAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGcc                                                                   >  1:2976516/1‑148 (MQ=255)
                                                                            tAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGcc                                                                   >  1:2976483/1‑148 (MQ=255)
                                                                            tAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGcc                                                                   >  1:842304/1‑148 (MQ=255)
                                                                                        gAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCAc                                                       >  2:1954511/1‑148 (MQ=255)
                                                                                         aaCCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCAcc                                                      <  2:550368/148‑1 (MQ=255)
                                                                                            cGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCAt                                                   <  1:1695689/148‑1 (MQ=255)
                                                                                            cGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCAt                                                   <  2:1092797/148‑1 (MQ=255)
                                                                                            cGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCAt                                                   <  2:2745094/148‑1 (MQ=255)
                                                                                              tATTCACCCGCCATCCCCATGCAGATGAACAGACGAGCGATAAACATGGTTATGTAGCCTTGCGCTAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGTGGCTGGTGACCATTGCCAGACGACGCCCGTAGCTGTCACCCATat                                                 <  2:3248117/148‑2 (MQ=255)
                                                                                                         cATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATc                                      <  2:2267655/148‑1 (MQ=255)
                                                                                                          aTCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCa                                     >  1:1372312/1‑148 (MQ=255)
                                                                                                               cATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgccg                                >  2:410347/1‑148 (MQ=255)
                                                                                                               cATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgccg                                >  2:409795/1‑148 (MQ=255)
                                                                                                               cATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgccg                                <  2:3012784/148‑1 (MQ=255)
                                                                                                                    cGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGaa                           <  2:1884968/148‑1 (MQ=255)
                                                                                                                        gACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAg                       >  2:2247295/1‑148 (MQ=255)
                                                                                                                         aCCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGc                      <  2:609641/148‑1 (MQ=255)
                                                                                                                            aGACGAGCGATAAACCTGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgag                   <  2:1939831/148‑1 (MQ=255)
                                                                                                                             gACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgaga                  <  1:3270046/148‑1 (MQ=255)
                                                                                                                             gACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgaga                  >  2:3013986/1‑148 (MQ=255)
                                                                                                                             gACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgaga                  >  2:607527/1‑148 (MQ=255)
                                                                                                                             gACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgaga                  >  1:935145/1‑148 (MQ=255)
                                                                                                                               cGAGCGATACACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgagaga                <  2:764913/148‑1 (MQ=255)
                                                                                                                               cGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgagaga                <  2:1466390/148‑1 (MQ=255)
                                                                                                                               cGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgagaga                <  2:2753676/148‑1 (MQ=255)
                                                                                                                               cGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCgagaga                <  2:1466587/148‑1 (MQ=255)
                                                                                                                                  gCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATaa             <  2:2314907/148‑1 (MQ=255)
                                                                                                                                   cGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATaaa            <  2:455412/148‑1 (MQ=255)
                                                                                                                                     aTAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAgg          <  2:623966/148‑1 (MQ=255)
                                                                                                                                     aTAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAgg          >  1:131020/1‑148 (MQ=255)
                                                                                                                                      tAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGc         >  1:1810748/1‑148 (MQ=255)
                                                                                                                                      tAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGc         >  1:633856/1‑148 (MQ=255)
                                                                                                                                       aaaCATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCt        <  1:400431/148‑1 (MQ=255)
                                                                                                                                       aaaCATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCt        >  1:2957651/1‑148 (MQ=255)
                                                                                                                                        aaCATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTg       >  1:1406694/1‑148 (MQ=255)
                                                                                                                                         aCATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGc      <  1:74003/148‑1 (MQ=255)
                                                                                                                                          cATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCa     >  1:172115/1‑148 (MQ=255)
                                                                                                                                          cATGGTTATGTAGCCTGGCGCAAAGCCACAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCa     >  2:2590618/1‑148 (MQ=255)
                                                                                                                                             ggTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCTCCGAACCAGCGAGAGATAAAGGCTGCagag  <  2:1059239/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAGAG  >  CP009273/3365463‑3365751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAGAGATCA  >  CP009273/3365463‑3365755
                                                                                                                                                |                                                                                                                                                    
GGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTA                                                                                                                                                 >  SRR19973070.2282713/1‑150 (MQ=60)
 GCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAG                                                                                                                                                <  SRR19973070.1505133/150‑1 (MQ=60)
   CCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCC                                                                                                                                              <  SRR19973070.923449/150‑1 (MQ=60)
    CCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCT                                                                                                                                             >  SRR19973070.168057/1‑150 (MQ=60)
    CCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCT                                                                                                                                             <  SRR19973070.139556/150‑1 (MQ=60)
      CACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGG                                                                                                                                           <  SRR19973070.1447788/150‑1 (MQ=60)
        CAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCG                                                                                                                                         <  SRR19973070.1610519/150‑1 (MQ=60)
        CAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCG                                                                                                                                         <  SRR19973070.1607276/150‑1 (MQ=60)
           AGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAA                                                                                                                                      >  SRR19973070.1020602/1‑150 (MQ=60)
           AGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAA                                                                                                                                      >  SRR19973070.3099633/1‑150 (MQ=60)
                    AAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGG                                                                                                                             >  SRR19973070.1334177/1‑150 (MQ=60)
                      ATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCC                                                                                                                           >  SRR19973070.735883/1‑150 (MQ=60)
                       TCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGATCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCA                                                                                                                          <  SRR19973070.2533823/150‑1 (MQ=60)
                       TCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGATCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCA                                                                                                                          <  SRR19973070.3004041/150‑1 (MQ=60)
                          AAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCG                                                                                                                       >  SRR19973070.613915/1‑150 (MQ=60)
                          AAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCG                                                                                                                       >  SRR19973070.1806687/1‑150 (MQ=60)
                          AAAAACCACTGGCTTTGTTACCCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTAATCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATCTAGCCTGGCGCAAAGCCGCAGGCCAGCG                                                                                                                       >  SRR19973070.614266/1‑150 (MQ=60)
                               CCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCG                                                                                                                  >  SRR19973070.2638856/1‑150 (MQ=60)
                               CCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCG                                                                                                                  >  SRR19973070.1454675/1‑150 (MQ=60)
                                  CTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCC                                                                                                               <  SRR19973070.508897/150‑1 (MQ=60)
                                         TGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGA                                                                                                        >  SRR19973070.554022/1‑150 (MQ=60)
                                          GTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAG                                                                                                       >  SRR19973070.2735714/1‑150 (MQ=60)
                                          GTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAG                                                                                                       >  SRR19973070.2000192/1‑150 (MQ=60)
                                             ACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAAC                                                                                                    <  SRR19973070.723739/150‑1 (MQ=60)
                                             ACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAAC                                                                                                    >  SRR19973070.1307240/1‑150 (MQ=60)
                                             ACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAAC                                                                                                    <  SRR19973070.2394195/150‑1 (MQ=60)
                                              CGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACG                                                                                                   >  SRR19973070.2249332/1‑150 (MQ=60)
                                               GCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGA                                                                                                  >  SRR19973070.1573893/1‑150 (MQ=60)
                                               GCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGA                                                                                                  >  SRR19973070.888172/1‑150 (MQ=60)
                                                CAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGAT                                                                                                 >  SRR19973070.1841465/1‑150 (MQ=60)
                                                  GATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGC                                                                                               >  SRR19973070.1897771/1‑150 (MQ=60)
                                                   ATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCT                                                                                              >  SRR19973070.3332284/1‑150 (MQ=60)
                                                           GCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCA                                                                                      <  SRR19973070.1855483/150‑1 (MQ=60)
                                                            CCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCAT                                                                                     >  SRR19973070.3172423/1‑150 (MQ=60)
                                                              AGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTG                                                                                   <  SRR19973070.80482/150‑1 (MQ=60)
                                                                CTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCC                                                                                 >  SRR19973070.2089402/1‑150 (MQ=60)
                                                                CTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCC                                                                                 <  SRR19973070.1067043/150‑1 (MQ=60)
                                                                  TTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAG                                                                               >  SRR19973070.1495251/1‑150 (MQ=60)
                                                                  TTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAG                                                                               <  SRR19973070.3245071/150‑1 (MQ=60)
                                                                     AATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACG                                                                            >  SRR19973070.1994904/1‑150 (MQ=60)
                                                                          CATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCC                                                                       <  SRR19973070.1989041/150‑1 (MQ=60)
                                                                          CATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCC                                                                       >  SRR19973070.2995622/1‑150 (MQ=60)
                                                                          CATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCC                                                                       >  SRR19973070.2995592/1‑150 (MQ=60)
                                                                          CATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCC                                                                       >  SRR19973070.2995589/1‑150 (MQ=60)
                                                                          CATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCC                                                                       >  SRR19973070.848015/1‑150 (MQ=60)
                                                                          CATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCC                                                                       >  SRR19973070.286932/1‑150 (MQ=60)
                                                                          CATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCC                                                                       <  SRR19973070.1687514/150‑1 (MQ=60)
                                                                           ATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCTTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCC                                                                      <  SRR19973070.1056652/150‑1 (MQ=60)
                                                                                            CGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAG                                                     <  SRR19973070.1707356/150‑1 (MQ=60)
                                                                                                        CCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCA                                         >  SRR19973070.1381641/1‑150 (MQ=60)
                                                                                                                           CAGACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGA                      >  SRR19973070.941815/1‑150 (MQ=60)
                                                                                                                             GACGAGCGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGA                    <  SRR19973070.3291076/150‑1 (MQ=60)
                                                                                                                                   CGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGG              >  SRR19973070.132169/1‑150 (MQ=60)
                                                                                                                                   CGATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGG              <  SRR19973070.617825/150‑1 (MQ=60)
                                                                                                                                    GATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGC             >  SRR19973070.1823229/1‑150 (MQ=60)
                                                                                                                                    GATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGC             >  SRR19973070.638359/1‑150 (MQ=60)
                                                                                                                                     ATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCT            >  SRR19973070.2976682/1‑150 (MQ=60)
                                                                                                                                     ATAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCT            >  SRR19973070.891102/1‑150 (MQ=60)
                                                                                                                                      TAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCTCCGAACCAGCGAGAGATAAAGGCTG           >  SRR19973070.358365/1‑150 (MQ=60)
                                                                                                                                      TAAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTG           >  SRR19973070.1416246/1‑150 (MQ=60)
                                                                                                                                       AAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGC          <  SRR19973070.403661/150‑1 (MQ=60)
                                                                                                                                       AAACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGC          <  SRR19973070.403372/150‑1 (MQ=60)
                                                                                                                                        AACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCA         >  SRR19973070.173494/1‑150 (MQ=60)
                                                                                                                                         ACATGGTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAG        <  SRR19973070.74801/150‑1 (MQ=60)
                                                                                                                                              GTTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAGAGATC   <  SRR19973070.489955/150‑1 (MQ=60)
                                                                                                                                               TTATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAGAGATCA  <  SRR19973070.694206/150‑1 (MQ=60)
                                                                                                                                                |                                                                                                                                                    
GGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTGGTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGCAGAGATCA  >  CP009273/3365463‑3365755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑