Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,373,457 C→A 91.3% intergenic (‑101/‑93) yhcM ← / → yhcB putative AFG1‑like family P‑loop ATPase/DUF1043 family inner membrane‑anchored protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,373,4570CA91.3% 387.2 / 29.5 146intergenic (‑101/‑93)yhcM/yhcBputative AFG1‑like family P‑loop ATPase/DUF1043 family inner membrane‑anchored protein
Reads supporting (aligned to +/- strand):  ref base C (8/5);  new base A (74/59);  total (82/64)
Fisher's exact test for biased strand distribution p-value = 7.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

TTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTG  >  CP009273/3373313‑3373598
                                                                                                                                                |                                                                                                                                             
ttAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTAtgt                                                                                                                                            >  1:503789/1‑148 (MQ=255)
     cgcCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAAt                                                                                                                                       >  2:2471108/1‑148 (MQ=255)
        cTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATCTGCAGTCGGGATTCCACTTCTGTGGAATTaa                                                                                                                                    >  1:1524872/1‑148 (MQ=255)
             ggTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGtt                                                                                                                               <  2:398505/148‑1 (MQ=255)
              gTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTta                                                                                                                              >  2:816709/1‑148 (MQ=255)
                aTTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTtata                                                                                                                            <  1:1041076/148‑1 (MQ=255)
                aTTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTtata                                                                                                                            <  1:1041104/148‑1 (MQ=255)
                  tGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGt                                                                                                                          >  1:390494/1‑148 (MQ=255)
                    cGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTgg                                                                                                                        <  2:3102476/148‑1 (MQ=255)
                      aTGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCa                                                                                                                      >  1:937901/1‑148 (MQ=255)
                        gTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATa                                                                                                                    >  2:439692/1‑148 (MQ=255)
                         ttGGGGTAACGCTTTGCCTGATCTTATTGTTAGTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATaa                                                                                                                   <  2:1780822/148‑1 (MQ=255)
                          tGGGGTAACGCTTTGCATGATCTTAGTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAAt                                                                                                                  <  2:2729217/148‑1 (MQ=255)
                           ggggTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATc                                                                                                                 <  2:440735/148‑1 (MQ=255)
                             ggTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAg                                                                                                               <  2:2892070/148‑1 (MQ=255)
                             ggTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAg                                                                                                               >  2:2310509/1‑148 (MQ=255)
                                    cTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAgg                                                                                                        >  2:288389/1‑148 (MQ=255)
                                     tttGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGc                                                                                                       <  1:2115068/148‑1 (MQ=255)
                                     tttGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGc                                                                                                       >  1:234700/1‑148 (MQ=255)
                                         cATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATgg                                                                                                   >  2:574890/1‑148 (MQ=255)
                                           tGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAg                                                                                                 >  1:3238350/1‑148 (MQ=255)
                                            gATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGc                                                                                                <  2:2137949/148‑1 (MQ=255)
                                            gATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGc                                                                                                <  1:3246103/148‑1 (MQ=255)
                                            gATCTTATTGTTAGTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGc                                                                                                <  1:1608324/148‑1 (MQ=255)
                                             aTCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGcc                                                                                               <  1:981249/148‑1 (MQ=255)
                                             aTCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGcc                                                                                               >  1:1665941/1‑148 (MQ=255)
                                              tCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCt                                                                                              >  2:95816/1‑148 (MQ=255)
                                                 tATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGaa                                                                                           >  1:2515335/1‑148 (MQ=255)
                                                 tATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGaa                                                                                           >  2:2745864/1‑148 (MQ=255)
                                                 tATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGaa                                                                                           >  1:2708968/1‑148 (MQ=255)
                                                  aTTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAg                                                                                          >  2:2187796/1‑148 (MQ=255)
                                                   gtGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGc                                                                                         <  2:1305483/147‑1 (MQ=255)
                                                    tGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGcc                                                                                        >  2:25228/1‑148 (MQ=255)
                                                      ttATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCaa                                                                                      <  1:2989637/148‑1 (MQ=255)
                                                       tATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAac                                                                                     >  1:2367251/1‑148 (MQ=255)
                                                          tCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACAcc                                                                                  >  1:1820703/1‑148 (MQ=255)
                                                          tCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACAcc                                                                                  >  1:318478/1‑148 (MQ=255)
                                                           ccTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACAccc                                                                                 <  2:1636581/148‑1 (MQ=255)
                                                            cTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCt                                                                                <  1:2742619/148‑1 (MQ=255)
                                                              tGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTAc                                                                              >  2:807833/1‑148 (MQ=255)
                                                               gAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACg                                                                             >  1:34508/1‑148 (MQ=255)
                                                                   aatCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGaaa                                                                         <  2:1917413/148‑1 (MQ=255)
                                                                     tCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACa                                                                       >  2:2930255/1‑148 (MQ=255)
                                                                     tCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACa                                                                       >  2:1599862/1‑148 (MQ=255)
                                                                     tCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACa                                                                       >  2:1593705/1‑148 (MQ=255)
                                                                      cGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTAAAAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACaa                                                                      >  2:2674536/1‑148 (MQ=255)
                                                                      cGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACaa                                                                      <  1:2671942/148‑1 (MQ=255)
                                                                      cGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACaa                                                                      >  2:822670/1‑148 (MQ=255)
                                                                      cGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACaa                                                                      >  1:3259825/1‑148 (MQ=255)
                                                                         tGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGATCCTGAAGCCAACACCCTACGGAAACAAAAg                                                                   >  1:2649485/1‑148 (MQ=255)
                                                                         tGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAg                                                                   <  1:2310509/148‑1 (MQ=255)
                                                                           cgcCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGAc                                                                 <  1:1260942/148‑1 (MQ=255)
                                                                             ccGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACaa                                                               <  2:2025364/148‑1 (MQ=255)
                                                                                ttgttCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACgg                                                            >  2:3263999/1‑148 (MQ=255)
                                                                                ttgttCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACgg                                                            >  2:942437/1‑148 (MQ=255)
                                                                                 tgttCACGGTTGACGAAAATAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACggg                                                           <  1:1840442/148‑1 (MQ=255)
                                                                                 tgttCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACggg                                                           >  2:410234/1‑148 (MQ=255)
                                                                                    tCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGgaga                                                        <  2:937901/148‑1 (MQ=255)
                                                                                      aCGGTTGACGAAAAAAAGGCCGTTCTAAACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATg                                                      <  1:2868197/148‑1 (MQ=255)
                                                                                        ggTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGtt                                                    <  2:1486359/148‑1 (MQ=255)
                                                                                         gTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTc                                                   >  2:906678/1‑148 (MQ=255)
                                                                                         gTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTc                                                   <  2:436029/148‑1 (MQ=255)
                                                                                           tGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCAt                                                 >  2:765342/1‑148 (MQ=255)
                                                                                            gACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATg                                                >  2:2757771/1‑148 (MQ=255)
                                                                                            gACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATg                                                >  1:270504/1‑148 (MQ=255)
                                                                                             aCGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGa                                               <  2:2943315/148‑1 (MQ=255)
                                                                                              cGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGAc                                              <  1:937229/148‑1 (MQ=255)
                                                                                               gAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGAcc                                             <  1:2855559/148‑1 (MQ=255)
                                                                                                 aaaaaaGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTg                                           >  2:2289865/1‑148 (MQ=255)
                                                                                                     aaGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGaa                                       <  1:291704/148‑1 (MQ=255)
                                                                                                     aaGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGaa                                       <  1:501629/148‑1 (MQ=255)
                                                                                                        gCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAatat                                    >  1:650117/1‑148 (MQ=255)
                                                                                                         ccGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATg                                   <  1:1975107/148‑1 (MQ=255)
                                                                                                         ccGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATg                                   <  2:2110388/148‑1 (MQ=255)
                                                                                                            ttCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATgcgc                                <  1:963366/148‑1 (MQ=255)
                                                                                                              cTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTa                              <  2:503789/148‑1 (MQ=255)
                                                                                                               tACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTaa                             <  1:2885177/148‑1 (MQ=255)
                                                                                                               tACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTaa                             >  2:2525865/1‑148 (MQ=255)
                                                                                                                acacTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAAt                            <  1:3263999/148‑1 (MQ=255)
                                                                                                                  acTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATNGTGGCATAATNAGCCGCANGCATGGNGCCTNAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTg                          >  2:1975699/1‑148 (MQ=255)
                                                                                                                  acTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTg                          <  1:1018274/148‑1 (MQ=255)
                                                                                                                  acTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTg                          <  2:1142005/148‑1 (MQ=255)
                                                                                                                  acTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTg                          <  2:2638130/148‑1 (MQ=255)
                                                                                                                   cTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTgg                         >  1:2572979/1‑148 (MQ=255)
                                                                                                                   cTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTgg                         >  1:2573118/1‑148 (MQ=255)
                                                                                                                    tACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTggg                        >  1:1252588/1‑148 (MQ=255)
                                                                                                                       gcgATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTa                     <  1:2493132/148‑1 (MQ=255)
                                                                                                                       gcgATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTa                     <  1:2449538/148‑1 (MQ=255)
                                                                                                                       gcgATATGCAGTCGGGATACCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTa                     <  1:1946796/148‑1 (MQ=255)
                                                                                                                        cgATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAg                    >  2:2395363/1‑148 (MQ=255)
                                                                                                                            atGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAgtcgt                <  1:536744/148‑1 (MQ=255)
                                                                                                                             tGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAgtcgtc               >  2:1000560/1‑148 (MQ=255)
                                                                                                                             tGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAgtcgtc               >  2:2653773/1‑148 (MQ=255)
                                                                                                                                    gggATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGcatca        <  1:1384792/148‑1 (MQ=255)
                                                                                                                                     ggATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGcatcat       >  1:937711/1‑148 (MQ=255)
                                                                                                                                     ggATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGcatcat       >  1:860822/1‑148 (MQ=255)
                                                                                                                                      gATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCatt      <  1:221511/148‑1 (MQ=255)
                                                                                                                                      gATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCatt      <  1:221499/148‑1 (MQ=255)
                                                                                                                                      gATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCatt      >  2:2666907/1‑148 (MQ=255)
                                                                                                                                          ccACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTg  <  1:3199685/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                             
TTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTG  >  CP009273/3373313‑3373598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTG  >  CP009273/3373311‑3373606
                                                                                                                                                  |                                                                                                                                                     
CATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGT                                                                                                                                                    >  SRR19973070.507282/1‑150 (MQ=60)
      GCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATT                                                                                                                                              <  SRR19973070.2380011/150‑1 (MQ=60)
        GCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATCTGCAGTCGGGATTCCACTTCTGTGGAATTAA                                                                                                                                            >  SRR19973070.1535580/1‑150 (MQ=60)
          CTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACG                                                                                                                                          >  SRR19973070.2962279/1‑150 (MQ=60)
              AGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTA                                                                                                                                      >  SRR19973070.2288406/1‑150 (MQ=60)
                  ATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGT                                                                                                                                  <  SRR19973070.1048426/150‑1 (MQ=60)
                  ATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGT                                                                                                                                  >  SRR19973070.393356/1‑150 (MQ=60)
                  ATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGT                                                                                                                                  <  SRR19973070.1048454/150‑1 (MQ=60)
                   TTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTG                                                                                                                                 <  SRR19973070.947913/150‑1 (MQ=60)
                      CGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCA                                                                                                                              >  SRR19973070.944583/1‑150 (MQ=60)
                         TGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAA                                                                                                                           >  SRR19973070.1884420/1‑150 (MQ=60)
                                     GCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGC                                                                                                               >  SRR19973070.236345/1‑150 (MQ=60)
                                       TTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCAT                                                                                                             <  SRR19973070.2129248/150‑1 (MQ=60)
                                           CATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAG                                                                                                         >  SRR19973070.3259249/1‑150 (MQ=60)
                                           CATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAG                                                                                                         >  SRR19973070.3259244/1‑150 (MQ=60)
                                             TGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCC                                                                                                       >  SRR19973070.1677388/1‑150 (MQ=60)
                                              GATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCT                                                                                                      <  SRR19973070.3267024/150‑1 (MQ=60)
                                              GATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTACACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCT                                                                                                      >  SRR19973070.503732/1‑150 (MQ=60)
                                              GATCTTATTGTTAGTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCT                                                                                                      <  SRR19973070.1619531/150‑1 (MQ=60)
                                               ATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTG                                                                                                     <  SRR19973070.988247/150‑1 (MQ=60)
                                                 CTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAA                                                                                                   >  SRR19973070.2726478/1‑150 (MQ=60)
                                                 CTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAA                                                                                                   >  SRR19973070.2531722/1‑150 (MQ=60)
                                                       GTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAAC                                                                                             >  SRR19973070.2382907/1‑150 (MQ=60)
                                                        TTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACA                                                                                            <  SRR19973070.3008791/150‑1 (MQ=60)
                                                          ATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACC                                                                                          >  SRR19973070.1833242/1‑150 (MQ=60)
                                                          ATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACC                                                                                          >  SRR19973070.320793/1‑150 (MQ=60)
                                                          ATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACC                                                                                          >  SRR19973070.320787/1‑150 (MQ=60)
                                                             CCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTA                                                                                       >  SRR19973070.2533621/1‑150 (MQ=60)
                                                              CTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTAC                                                                                      <  SRR19973070.2760266/150‑1 (MQ=60)
                                                              CTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTAC                                                                                      <  SRR19973070.2760259/150‑1 (MQ=60)
                                                               TTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACG                                                                                     >  SRR19973070.34956/1‑150 (MQ=60)
                                                                    TAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAAC                                                                                <  SRR19973070.3300380/150‑1 (MQ=60)
                                                                    TAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAAC                                                                                >  SRR19973070.520964/1‑150 (MQ=60)
                                                                      ATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAA                                                                              >  SRR19973070.3280816/1‑150 (MQ=60)
                                                                      ATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAA                                                                              >  SRR19973070.1716044/1‑150 (MQ=60)
                                                                        CGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAA                                                                            <  SRR19973070.2689297/150‑1 (MQ=60)
                                                                         GGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGATCCTGAAGCCAACACCCTACGGAAACAAAAG                                                                           >  SRR19973070.2666728/1‑150 (MQ=60)
                                                                           TGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGAC                                                                         <  SRR19973070.2325867/150‑1 (MQ=60)
                                                                             CGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAA                                                                       <  SRR19973070.1269781/150‑1 (MQ=60)
                                                                                CGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGG                                                                    >  SRR19973070.1199041/1‑150 (MQ=60)
                                                                                   TGTTCACGGTTGACGAAAATAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAG                                                                 <  SRR19973070.1853077/150‑1 (MQ=60)
                                                                                        ACGGTTGACGAAAAAAAGGCCGTTCTAAACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTT                                                            <  SRR19973070.2886778/150‑1 (MQ=60)
                                                                                            TTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATG                                                        >  SRR19973070.272390/1‑150 (MQ=60)
                                                                                            TTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATG                                                        >  SRR19973070.272280/1‑150 (MQ=60)
                                                                                             TGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGA                                                       >  SRR19973070.137/1‑150 (MQ=60)
                                                                                                CGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCT                                                    <  SRR19973070.943909/150‑1 (MQ=60)
                                                                                                CGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCT                                                    <  SRR19973070.943911/150‑1 (MQ=60)
                                                                                                 GAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTG                                                   <  SRR19973070.2874057/150‑1 (MQ=60)
                                                                                                  AAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGG                                                  >  SRR19973070.216269/1‑150 (MQ=60)
                                                                                                       AAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATA                                             <  SRR19973070.293695/150‑1 (MQ=60)
                                                                                                       AAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATA                                             <  SRR19973070.505109/150‑1 (MQ=60)
                                                                                                        AGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATAT                                            >  SRR19973070.654750/1‑150 (MQ=60)
                                                                                                        AGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATAT                                            <  SRR19973070.2487213/150‑1 (MQ=60)
                                                                                                           CCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCG                                         <  SRR19973070.1988465/150‑1 (MQ=60)
                                                                                                           CCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCG                                         <  SRR19973070.81504/150‑1 (MQ=60)
                                                                                                              TTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTA                                      <  SRR19973070.970247/150‑1 (MQ=60)
                                                                                                                 TACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATT                                   <  SRR19973070.2903856/150‑1 (MQ=60)
                                                                                                                  ACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTG                                  <  SRR19973070.3285007/150‑1 (MQ=60)
                                                                                                                   CACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGG                                 >  SRR19973070.2589793/1‑150 (MQ=60)
                                                                                                                   CACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGG                                 >  SRR19973070.2589932/1‑150 (MQ=60)
                                                                                                                    ACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGG                                >  SRR19973070.1261374/1‑150 (MQ=60)
                                                                                                                    ACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGG                                <  SRR19973070.1025506/150‑1 (MQ=60)
                                                                                                                      TACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTT                              <  SRR19973070.290330/150‑1 (MQ=60)
                                                                                                                         GCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGT                           <  SRR19973070.2509305/150‑1 (MQ=60)
                                                                                                                         GCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGT                           <  SRR19973070.2465560/150‑1 (MQ=60)
                                                                                                                         GCGATATGCAGTCGGGATACCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGT                           <  SRR19973070.1959870/150‑1 (MQ=60)
                                                                                                                              ATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCG                      <  SRR19973070.540345/150‑1 (MQ=60)
                                                                                                                                    TCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCA                <  SRR19973070.1609534/150‑1 (MQ=60)
                                                                                                                                     CGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCAT               >  SRR19973070.866795/1‑150 (MQ=60)
                                                                                                                                     CGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCAT               >  SRR19973070.2609601/1‑150 (MQ=60)
                                                                                                                                     CGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCAT               >  SRR19973070.944392/1‑150 (MQ=60)
                                                                                                                                      GGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATT              <  SRR19973070.1394163/150‑1 (MQ=60)
                                                                                                                                        GATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTAT            <  SRR19973070.223115/150‑1 (MQ=60)
                                                                                                                                        GATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTAT            <  SRR19973070.223102/150‑1 (MQ=60)
                                                                                                                                            CCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGT        <  SRR19973070.3220389/150‑1 (MQ=60)
                                                                                                                                                  ATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTG  <  SRR19973070.442881/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
CATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTG  >  CP009273/3373311‑3373606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑