Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F527 I0 R1	1651	322.4	10661268	95.2%	10149527	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,373,457	C→A	100%	intergenic (‑101/‑93)	yhcM ← / → yhcB	putative AFG1‑like family P‑loop ATPase/DUF1043 family inner membrane‑anchored protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,373,457	0	C	A	98.9%	886.3 / ‑0.0	283	intergenic (‑101/‑93)	yhcM/yhcB	putative AFG1‑like family P‑loop ATPase/DUF1043 family inner membrane‑anchored protein
Reads supporting (aligned to +/- strand): ref base C (1/3); new base A (142/137); total (143/140)
Fisher's exact test for biased strand distribution p-value = 3.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 283 total aligned reads displayed.

TCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCT  >  CP009273/3373310‑3373603

      aGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGGTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAAt                                                                                                                                            <  1:123894/150‑1 (MQ=255)

        cgcCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTa                                                                                                                                          <  2:3898410/150‑1 (MQ=255)

        cgcCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATATTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTa                                                                                                                                          <  1:1657867/150‑1 (MQ=255)

          ccTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAAc                                                                                                                                        <  2:4562215/150‑1 (MQ=255)

          ccTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAAc                                                                                                                                        <  1:1840321/150‑1 (MQ=255)

            ttCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACgg                                                                                                                                      <  2:28728/150‑1 (MQ=255)

             tCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGt                                                                                                                                     <  2:1972821/150‑1 (MQ=255)

              cAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGtt                                                                                                                                    <  1:2167004/150‑1 (MQ=255)

               aGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTta                                                                                                                                   <  1:4010355/150‑1 (MQ=255)

                ggTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTtat                                                                                                                                  <  1:1934551/150‑1 (MQ=255)

                 gTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTtata                                                                                                                                 <  1:2069802/150‑1 (MQ=255)

                 gTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTtata                                                                                                                                 <  1:4144533/150‑1 (MQ=255)

                  tATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAg                                                                                                                                >  2:3669623/1‑150 (MQ=255)

                  tATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATGCCTTGAAGAATCGGTGCGCCGGTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAg                                                                                                                                <  2:155167/150‑1 (MQ=255)

                   aNTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAGAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGt                                                                                                                               >  1:4222861/1‑150 (MQ=255)

                    gtGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTg                                                                                                                              <  2:2046316/149‑1 (MQ=255)

                    gtGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTg                                                                                                                              <  2:4286212/149‑1 (MQ=255)

                         aTGTTTGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATa                                                                                                                         <  1:3672797/150‑1 (MQ=255)

                         aTGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATa                                                                                                                         >  1:4360842/1‑150 (MQ=255)

                         aTGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGACATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATa                                                                                                                         <  1:3498564/150‑1 (MQ=255)

                             tGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCa                                                                                                                     >  1:1676147/1‑150 (MQ=255)

                              ggggTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAg                                                                                                                    >  2:729459/1‑150 (MQ=255)

                                  tAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGc                                                                                                                >  2:2115894/1‑150 (MQ=255)

                                      gCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGc                                                                                                            <  2:947078/150‑1 (MQ=255)

                                       cTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCa                                                                                                           >  2:2180726/1‑150 (MQ=255)

                                       cTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCa                                                                                                           >  1:3231639/1‑150 (MQ=255)

                                          tGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATgg                                                                                                        <  1:4284617/150‑1 (MQ=255)

                                          tGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATgg                                                                                                        <  1:464900/150‑1 (MQ=255)

                                            cATGATCTTATTGTTATTCCTTGAATATTCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAg                                                                                                      <  1:3954300/150‑1 (MQ=255)

                                            cATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAg                                                                                                      >  2:4316024/1‑150 (MQ=255)

                                            cATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAg                                                                                                      <  2:1161608/150‑1 (MQ=255)

                                             aTGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGc                                                                                                     >  2:3709995/1‑150 (MQ=255)

                                                aTCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTg                                                                                                  >  1:78877/1‑150 (MQ=255)

                                                  cTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACTGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGaa                                                                                                >  1:3912000/1‑150 (MQ=255)

                                                    tATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGc                                                                                              >  2:3078019/1‑150 (MQ=255)

                                                     aTTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGcc                                                                                             >  2:1276526/1‑150 (MQ=255)

                                                      ttGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCa                                                                                            >  1:136490/1‑150 (MQ=255)

                                                          tATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAacac                                                                                        >  1:3302136/1‑150 (MQ=255)

                                                          tATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAacac                                                                                        <  2:2682354/150‑1 (MQ=255)

                                                          tATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAacac                                                                                        <  2:3294467/150‑1 (MQ=255)

                                                              ccTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTa                                                                                    <  1:448990/150‑1 (MQ=255)

                                                              ccTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTa                                                                                    >  2:414017/1‑150 (MQ=255)

                                                                 tGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACgg                                                                                 >  1:575107/1‑150 (MQ=255)

                                                                 tGAATAATCGGTGCACCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACgg                                                                                 <  2:1426227/150‑1 (MQ=255)

                                                                   aataatCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGaa                                                                               >  1:2201027/1‑150 (MQ=255)

                                                                    ataatCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGaaa                                                                              >  2:2472271/1‑150 (MQ=255)

                                                                     taatCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAAc                                                                             <  2:3943300/150‑1 (MQ=255)

                                                                     taatCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAAc                                                                             >  2:1337513/1‑150 (MQ=255)

                                                                      aatCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACa                                                                            >  2:3024477/1‑150 (MQ=255)

                                                                        tCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACaaa                                                                          <  1:4658160/150‑1 (MQ=255)

                                                                         cGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGACTTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACaaaa                                                                         >  2:1716152/1‑150 (MQ=255)

                                                                          ggTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAg                                                                        <  2:5171568/150‑1 (MQ=255)

                                                                            tGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGAc                                                                      >  2:5210789/1‑150 (MQ=255)

                                                                             gcgcCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACa                                                                     <  2:4240717/150‑1 (MQ=255)

                                                                               gcCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAAc                                                                   >  1:3060635/1‑150 (MQ=255)

                                                                               gcCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAAc                                                                   >  2:3624641/1‑150 (MQ=255)

                                                                               gcCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAAc                                                                   <  2:3798107/150‑1 (MQ=255)

                                                                                ccGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACg                                                                  <  2:2233339/150‑1 (MQ=255)

                                                                                ccGGTGGTCTCGGTTGACGAACAAAAGGCCGTTCTACACTTCGCGATATGCCGTCGGGATTCCTCTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACg                                                                  <  2:320799/150‑1 (MQ=255)

                                                                                 cGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACgg                                                                 >  1:3037074/1‑150 (MQ=255)

                                                                                     gttCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGgaga                                                             <  1:718728/150‑1 (MQ=255)

                                                                                     gttCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTACACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGgaga                                                             >  2:2400020/1‑150 (MQ=255)

                                                                                       tCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATg                                                           <  2:4635887/150‑1 (MQ=255)

                                                                                         aCGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGtt                                                         <  1:2945700/150‑1 (MQ=255)

                                                                                            gTTGACGAAAAAAAGGCCGTTCTACACTACTCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCAt                                                      <  2:4491014/150‑1 (MQ=255)

                                                                                                aCGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGAcc                                                  <  2:3251527/150‑1 (MQ=255)

                                                                                                aCGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCACAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGAcc                                                  <  2:4721704/150‑1 (MQ=255)

                                                                                                  gAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTg                                                <  1:4838011/150‑1 (MQ=255)

                                                                                                  gAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTg                                                >  1:514225/1‑150 (MQ=255)

                                                                                                   aaaaaaaGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTgg                                               >  1:2258013/1‑150 (MQ=255)

                                                                                                    aaaaaaGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTggg                                              >  2:3995969/1‑150 (MQ=255)

                                                                                                     aaaaaGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGa                                             <  2:842515/150‑1 (MQ=255)

                                                                                                      aaaaGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGaa                                            >  2:3411355/1‑150 (MQ=255)

                                                                                                         aGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAatat                                         <  1:455228/150‑1 (MQ=255)

                                                                                                         aGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAatat                                         >  2:3894708/1‑150 (MQ=255)

                                                                                                             cGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATgcgc                                     >  1:1148989/1‑150 (MQ=255)

                                                                                                              gTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCt                                    >  1:2445019/1‑150 (MQ=255)

                                                                                                              gTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCt                                    <  2:5320848/150‑1 (MQ=255)

                                                                                                                tCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTaa                                  >  2:72649/1‑150 (MQ=255)

                                                                                                                tCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTaa                                  >  2:5118381/1‑150 (MQ=255)

                                                                                                                 cTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAAt                                 <  1:4167778/150‑1 (MQ=255)

                                                                                                                 cTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAAt                                 >  1:3758919/1‑150 (MQ=255)

                                                                                                                   acacTACGCGATATGCAGTCGGGATTCCACTTATGTGGCCTTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGACACAAAAGACACCGGGAGATGTTCATGACCTGGGAATATGCGCtcagtg                               >  2:4601451/1‑145 (MQ=255)

                                                                                                                      cTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGt                            >  1:981615/1‑150 (MQ=255)

                                                                                                                      cTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGt                            >  2:620585/1‑150 (MQ=255)

                                                                                                                       tACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGtt                           <  2:1868055/150‑1 (MQ=255)

                                                                                                                        aCGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTa                          <  2:1312989/150‑1 (MQ=255)

                                                                                                                        aCGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTa                          >  1:5247013/1‑150 (MQ=255)

                                                                                                                          gcgATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAgt                        >  1:4797010/1‑150 (MQ=255)

                                                                                                                             atatGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAgtcgt                     >  1:5040666/1‑150 (MQ=255)

                                                                                                                              tatGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAgtcgtc                    >  1:1500985/1‑150 (MQ=255)

                                                                                                                                   cgTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGcat               >  2:1380709/2‑150 (MQ=255)

                                                                                                                                       gggATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCatt           <  1:941789/150‑1 (MQ=255)

                                                                                                                                       gggATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCatt           <  2:2495518/150‑1 (MQ=255)

                                                                                                                                        ggATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCatta          >  1:4727025/1‑150 (MQ=255)

                                                                                                                                         gATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCattat         >  2:4084659/1‑150 (MQ=255)

                                                                                                                                           ttCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTg       >  1:2322107/1‑150 (MQ=255)

                                                                                                                                           ttCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTg       <  2:5017315/150‑1 (MQ=255)

                                                                                                                                            tCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTgg      <  2:2296949/150‑1 (MQ=255)

                                                                                                                                                cTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGAATTTAATTACCAGTGAATATGCGCTAATTCTGATAGTCGACGGCATCATTATTgctgct  >  2:278153/1‑150 (MQ=255)

TCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCT  >  CP009273/3373310‑3373603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCT  >  CP009273/3373308‑3373603

CTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAATAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCAACTTA                                                                                                                                                    >  SRR19973062.1843274/1‑150 (MQ=60)

 TTCATTAAGCGCCTTCAGGTATTGCTATTTTTGGGTAACGCTTTGCATGATCTTTTTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCTCGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTAT                                                                                                                                                   <  SRR19973062.1406349/150‑1 (MQ=60)

 TTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTAT                                                                                                                                                   <  SRR19973062.3348666/150‑1 (MQ=60)

 TTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTAT                                                                                                                                                   <  SRR19973062.4300591/150‑1 (MQ=60)

 TTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTAT                                                                                                                                                   <  SRR19973062.4300584/150‑1 (MQ=60)

 TTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTAAGCGATATGCCGTCGGGATTCCACTTAT                                                                                                                                                   >  SRR19973062.2244509/1‑150 (MQ=60)

    ATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTG                                                                                                                                                >  SRR19973062.3664583/1‑150 (MQ=60)

      TAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGA                                                                                                                                              >  SRR19973062.3736406/1‑150 (MQ=60)

        AGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAAT                                                                                                                                            <  SRR19973062.125086/150‑1 (MQ=60)

        AGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGGTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAAT                                                                                                                                            <  SRR19973062.124647/150‑1 (MQ=60)

          CGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTA                                                                                                                                          >  SRR19973062.2668871/1‑150 (MQ=60)

          CGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATATTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTA                                                                                                                                          <  SRR19973062.1666020/150‑1 (MQ=60)

            CCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAAC                                                                                                                                        <  SRR19973062.1849481/150‑1 (MQ=60)

                CAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTT                                                                                                                                    <  SRR19973062.2177950/150‑1 (MQ=60)

                 AGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTA                                                                                                                                   <  SRR19973062.4030595/150‑1 (MQ=60)

                  GGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTAT                                                                                                                                  <  SRR19973062.1944475/150‑1 (MQ=60)

                   GTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATA                                                                                                                                 <  SRR19973062.2080396/150‑1 (MQ=60)

                   GTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATA                                                                                                                                 <  SRR19973062.4166020/150‑1 (MQ=60)

                     ANTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAGAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGT                                                                                                                               >  SRR19973062.4244749/1‑150 (MQ=60)

                           ATGTTTGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATA                                                                                                                         <  SRR19973062.3691629/150‑1 (MQ=60)

                           ATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATA                                                                                                                         >  SRR19973062.328270/1‑150 (MQ=60)

                           ATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATA                                                                                                                         >  SRR19973062.4383097/1‑150 (MQ=60)

                           ATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATA                                                                                                                         >  SRR19973062.4383233/1‑150 (MQ=60)

                           ATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATAAACGGTTATAGTGGCATA                                                                                                                         >  SRR19973062.3935891/1‑150 (MQ=60)

                           ATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGACATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATA                                                                                                                         <  SRR19973062.3516320/150‑1 (MQ=60)

                            TGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAA                                                                                                                        >  SRR19973062.2403682/1‑150 (MQ=60)

                               TGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCA                                                                                                                     >  SRR19973062.1684381/1‑150 (MQ=60)

                               TGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCA                                                                                                                     >  SRR19973062.4671298/1‑150 (MQ=60)

                                      ACTCTTTTCATGATATTATTGTTATTCCTTGAATTATCGTTGCGCCGTTGTTCACGGTTGACTAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCTATTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAG                                                                                                              <  SRR19973062.5241953/150‑1 (MQ=60)

                                       CGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGG                                                                                                             <  SRR19973062.5318494/150‑1 (MQ=60)

                                       CGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGG                                                                                                             >  SRR19973062.1952910/1‑150 (MQ=60)

                                         CTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCA                                                                                                           >  SRR19973062.3248410/1‑150 (MQ=60)

                                          TTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGACATAATCAGCCGCAGGCAT                                                                                                          <  SRR19973062.3216907/150‑1 (MQ=60)

                                            TGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGG                                                                                                        <  SRR19973062.4306766/150‑1 (MQ=60)

                                            TGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGG                                                                                                        <  SRR19973062.1857145/150‑1 (MQ=60)

                                            TGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGG                                                                                                        <  SRR19973062.466735/150‑1 (MQ=60)

                                            TGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGG                                                                                                        <  SRR19973062.466965/150‑1 (MQ=60)

                                              CATGATCTTATTGTTATTCCTTGAATATTCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAG                                                                                                      <  SRR19973062.3974365/150‑1 (MQ=60)

                                              CATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAG                                                                                                      >  SRR19973062.4266600/1‑150 (MQ=60)

                                              CATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAG                                                                                                      >  SRR19973062.731693/1‑150 (MQ=60)

                                               ATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGC                                                                                                     >  SRR19973062.4963250/1‑150 (MQ=60)

                                               ATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGC                                                                                                     >  SRR19973062.2308889/1‑150 (MQ=60)

                                                  ATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTG                                                                                                  >  SRR19973062.334481/1‑150 (MQ=60)

                                                  ATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTG                                                                                                  >  SRR19973062.79395/1‑150 (MQ=60)

                                                  ATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTG                                                                                                  >  SRR19973062.2115379/1‑150 (MQ=60)

                                                    CTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACTGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAA                                                                                                >  SRR19973062.3931918/1‑150 (MQ=60)

                                                     TTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAG                                                                                               >  SRR19973062.3331387/1‑150 (MQ=60)

                                                     TTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAG                                                                                               >  SRR19973062.305148/1‑150 (MQ=60)

                                                      TATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGC                                                                                              >  SRR19973062.915430/1‑150 (MQ=60)

                                                       ATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCC                                                                                             <  SRR19973062.4008682/150‑1 (MQ=60)

                                                        TTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCA                                                                                            >  SRR19973062.137298/1‑150 (MQ=60)

                                                          GTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAAC                                                                                          <  SRR19973062.4778151/150‑1 (MQ=60)

                                                           TTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACA                                                                                         >  SRR19973062.2223504/1‑150 (MQ=60)

                                                            TATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACAC                                                                                        >  SRR19973062.3319231/1‑150 (MQ=60)

                                                               TCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCT                                                                                     <  SRR19973062.835695/150‑1 (MQ=60)

                                                               TCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCT                                                                                     <  SRR19973062.3817022/150‑1 (MQ=60)

                                                                CCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTA                                                                                    <  SRR19973062.450921/150‑1 (MQ=60)

                                                                CCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTA                                                                                    <  SRR19973062.947516/150‑1 (MQ=60)

                                                                CCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTA                                                                                    >  SRR19973062.3749243/1‑150 (MQ=60)

                                                                 CTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTAC                                                                                   >  SRR19973062.2259603/1‑150 (MQ=60)

                                                                 CTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTAC                                                                                   <  SRR19973062.1508098/150‑1 (MQ=60)

                                                                  TTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACG                                                                                  <  SRR19973062.3061526/150‑1 (MQ=60)

                                                                   TGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGG                                                                                 >  SRR19973062.577799/1‑150 (MQ=60)

                                                                     AATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAA                                                                               >  SRR19973062.424013/1‑150 (MQ=60)

                                                                     AATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAA                                                                               >  SRR19973062.2212068/1‑150 (MQ=60)

                                                                      ATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCAACGGAAA                                                                              >  SRR19973062.5169015/1‑150 (MQ=60)

                                                                       TAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAAC                                                                             <  SRR19973062.4437213/150‑1 (MQ=60)

                                                                        AATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACA                                                                            >  SRR19973062.4875943/1‑150 (MQ=60)

                                                                        AATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACA                                                                            >  SRR19973062.5172083/1‑150 (MQ=60)

                                                                          TCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAA                                                                          <  SRR19973062.4682527/150‑1 (MQ=60)

                                                                            GGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAG                                                                        <  SRR19973062.1750528/150‑1 (MQ=60)

                                                                            GGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAG                                                                        >  SRR19973062.4571669/1‑150 (MQ=60)

                                                                            GGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAG                                                                        >  SRR19973062.4571785/1‑150 (MQ=60)

                                                                             GTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGA                                                                       <  SRR19973062.4175616/150‑1 (MQ=60)

                                                                                 GCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAAC                                                                   >  SRR19973062.3076517/1‑150 (MQ=60)

                                                                                 GCCGTTGTACACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAAC                                                                   >  SRR19973062.3934603/1‑150 (MQ=60)

                                                                                   CGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGG                                                                 >  SRR19973062.3052885/1‑150 (MQ=60)

                                                                                   CGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGG                                                                 >  SRR19973062.3052954/1‑150 (MQ=60)

                                                                                     TTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGA                                                               <  SRR19973062.195370/150‑1 (MQ=60)

                                                                                      TGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCCTGGAGCCTGAAGCCAACACCCAACGGAAACAAAAGACAACGGGAG                                                              >  SRR19973062.136189/1‑150 (MQ=60)

                                                                                       TTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGTCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGA                                                             <  SRR19973062.4172506/150‑1 (MQ=60)

                                                                                       GTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGA                                                             <  SRR19973062.722020/150‑1 (MQ=60)

                                                                                         TCACGGTTGACGAAAAAAAGGCCGTTCTACACTTCGCGATATGCCGTCGGGATTCCACTTATGTGGATTTAACGGTTTTAGTGGCATTATCAGCCGCAGGCATGGAGCCTGATGCCAACACCCTACGGAAACAAAAGACAACGGGAGATG                                                           <  SRR19973062.473678/150‑1 (MQ=60)

                                                                                         TCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATG                                                           <  SRR19973062.473700/150‑1 (MQ=60)

                                                                                           ACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTT                                                         <  SRR19973062.2961191/150‑1 (MQ=60)

                                                                                                   CGAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCT                                                 >  SRR19973062.4108450/1‑150 (MQ=60)

                                                                                                    GAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTG                                                <  SRR19973062.4863017/150‑1 (MQ=60)

                                                                                                    GAAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTG                                                <  SRR19973062.4339638/150‑1 (MQ=60)

                                                                                                    GAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTG                                                >  SRR19973062.516637/1‑150 (MQ=60)

                                                                                                     AAAAAAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGG                                               >  SRR19973062.2269357/1‑150 (MQ=60)

                                                                                                     AAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGG                                               >  SRR19973062.1593424/1‑150 (MQ=60)

                                                                                                     AAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGG                                               <  SRR19973062.1677660/150‑1 (MQ=60)

                                                                                                         AAAGGCCGTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAAT                                           <  SRR19973062.1430063/150‑1 (MQ=60)

                                                                                                          AAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATA                                          >  SRR19973062.3846571/1‑150 (MQ=60)

                                                                                                           AGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATAT                                         <  SRR19973062.457198/150‑1 (MQ=60)

                                                                                                               CGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGC                                     >  SRR19973062.1154368/1‑150 (MQ=60)

                                                                                                                GTTCTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCT                                    >  SRR19973062.2458137/1‑150 (MQ=60)

                                                                                                                 TTCTACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTA                                   <  SRR19973062.1530318/150‑1 (MQ=60)

                                                                                                                   CTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAAT                                 <  SRR19973062.42897/150‑1 (MQ=60)

                                                                                                                   CTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAAT                                 >  SRR19973062.3778188/1‑150 (MQ=60)

                                                                                                                   CTACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAAT                                 <  SRR19973062.4189406/150‑1 (MQ=60)

                                                                                                                    TACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATT                                <  SRR19973062.4676952/150‑1 (MQ=60)

                                                                                                                    TACACTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATT                                <  SRR19973062.538917/150‑1 (MQ=60)

                                                                                                                     ACACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTG                               >  SRR19973062.2193340/1‑150 (MQ=60)

                                                                                                                      CACTACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGG                              >  SRR19973062.4674647/1‑150 (MQ=60)

                                                                                                                        CTACGCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGT                            >  SRR19973062.986169/1‑150 (MQ=60)

                                                                                                                          ACGCGATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTA                          >  SRR19973062.5274645/1‑150 (MQ=60)

                                                                                                                            GCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGT                        >  SRR19973062.4821895/1‑150 (MQ=60)

                                                                                                                            GCGATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGGTCATGACCTGGGACTATGCGCGAATTGGGTTAGT                        >  SRR19973062.4821923/1‑150 (MQ=60)

                                                                                                                              GATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCG                      >  SRR19973062.1679485/1‑150 (MQ=60)

                                                                                                                               ATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGT                     >  SRR19973062.5067383/1‑150 (MQ=60)

                                                                                                                               ATATGCCGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGT                     >  SRR19973062.5067431/1‑150 (MQ=60)

                                                                                                                               ATATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGT                     >  SRR19973062.2206953/1‑150 (MQ=60)

                                                                                                                                TATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTC                    >  SRR19973062.1508361/1‑150 (MQ=60)

                                                                                                                                 cggccGTCGGGACTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTCCGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCG                   <  SRR19973062.4046597/145‑1 (MQ=60)

                                                                                                                                 ATGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCG                   <  SRR19973062.3012830/150‑1 (MQ=60)

                                                                                                                                  TGCAGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGG                  >  SRR19973062.3650739/1‑150 (MQ=60)

                                                                                                                                     CGTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCAT               >  SRR19973062.2732648/1‑150 (MQ=60)

                                                                                                                                      GTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATC              <  SRR19973062.587650/150‑1 (MQ=60)

                                                                                                                                      GTCGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATC              >  SRR19973062.1900776/1‑150 (MQ=60)

                                                                                                                                        CGGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCAT            <  SRR19973062.2126660/150‑1 (MQ=60)

                                                                                                                                         GGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATT           <  SRR19973062.946044/150‑1 (MQ=60)

                                                                                                                                         GGGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATT           <  SRR19973062.4763955/150‑1 (MQ=60)

                                                                                                                                          GGATTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTA          >  SRR19973062.4751690/1‑150 (MQ=60)

                                                                                                                                             TTCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTG       >  SRR19973062.2334374/1‑150 (MQ=60)

                                                                                                                                              TCCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGG      >  SRR19973062.2384910/1‑150 (MQ=60)

                                                                                                                                               CCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGT     <  SRR19973062.3237773/150‑1 (MQ=60)

                                                                                                                                               CCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGT     <  SRR19973062.4202791/150‑1 (MQ=60)

                                                                                                                                               CCACTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGT     >  SRR19973062.1178187/1‑150 (MQ=60)

                                                                                                                                                CACTTATGTGTAATTAACTGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTG    <  SRR19973062.976721/150‑1 (MQ=60)

                                                                                                                                                  CTTATGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCT  >  SRR19973062.622777/1‑150 (MQ=60)

CTTCATTAAGCGCCTTCAGGTATTGCGATGTTGGGGTAACGCTTTGCATGATCTTATTGTTATTCCTTGAATAATCGGTGCGCCGTTGTTCACGGTTGACGAAAAAAAGGCCGTTCTACACTACGCGATATGCAGTCGGGATTCCACTTCTGTGGAATTAACGGTTATAGTGGCATAATCAGCCGCAGGCATGGAGCCTGAAGCCAACACCCTACGGAAACAAAAGACAACGGGAGATGTTCATGACCTGGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCT  >  CP009273/3373308‑3373603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`