Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,767,410 G→T 87.6% V590V (GTG→GTT)  mtlA → fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,767,4100GT87.6% 428.0 / 60.1 178V590V (GTG→GTT) mtlAfused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components
Reads supporting (aligned to +/- strand):  ref base G (8/14);  new base T (75/81);  total (83/95)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 178 total aligned reads displayed.

CCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAA  >  CP009273/3767267‑3767554
                                                                                                                                               |                                                                                                                                                
ccGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTg                                                                                                                                              <  1:2811322/148‑1 (MQ=255)
ccGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTg                                                                                                                                              <  2:3295389/148‑1 (MQ=255)
 cGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTgg                                                                                                                                             >  1:963994/1‑148 (MQ=255)
 cGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTgg                                                                                                                                             <  2:1506564/148‑1 (MQ=255)
  gtcTTTTCTGGGTTAGTCTTTCGCGTTTAGTCTCTGTACTGTTGAAGCTATAGTTCGCGTACTGAATAGTGGCGTCGTGTTGTGCCTTTATCCGGAAGGCGTGCGCTTCGGTGAAGAAGAATATGACAGTTCGCGTATGGTTATTgat                                                                                                                                            <  2:1079336/146‑3 (MQ=255)
   aCTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTa                                                                                                                                           <  2:1637864/148‑1 (MQ=255)
   aCTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTa                                                                                                                                           <  2:1214886/148‑1 (MQ=255)
      tATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtg                                                                                                                                        <  2:1668082/148‑1 (MQ=255)
      tATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtg                                                                                                                                        >  2:1433083/1‑148 (MQ=255)
         cTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtgctg                                                                                                                                     >  1:1495931/1‑148 (MQ=255)
           gggTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGcc                                                                                                                                   >  1:537933/1‑148 (MQ=255)
            ggTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAGCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGccc                                                                                                                                  >  1:2280460/1‑148 (MQ=255)
             gTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCg                                                                                                                                 >  1:1265062/1‑148 (MQ=255)
              tGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTTCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGt                                                                                                                                >  1:3215013/1‑148 (MQ=255)
                aGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTaa                                                                                                                              <  2:1175671/148‑1 (MQ=255)
                  tCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTaaca                                                                                                                            <  2:346409/148‑1 (MQ=255)
                    tATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTaacaac                                                                                                                          >  2:462181/1‑148 (MQ=255)
                     aTCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACg                                                                                                                         <  2:3092566/148‑1 (MQ=255)
                       cgcgGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAg                                                                                                                       <  1:899426/148‑1 (MQ=255)
                       cgcgGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAg                                                                                                                       <  1:2555275/148‑1 (MQ=255)
                        gcgGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACACGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGc                                                                                                                      <  1:1252927/148‑1 (MQ=255)
                          ggTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGcac                                                                                                                    <  2:1311743/148‑1 (MQ=255)
                                acacGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAg                                                                                                              >  2:466212/1‑148 (MQ=255)
                                  acggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGt                                                                                                            <  2:1336311/148‑1 (MQ=255)
                                  acggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGt                                                                                                            <  2:725890/148‑1 (MQ=255)
                                  acggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGt                                                                                                            <  2:2386094/148‑1 (MQ=255)
                                   cggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGtt                                                                                                           >  1:101125/1‑148 (MQ=255)
                                   cggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGtt                                                                                                           <  2:1899705/148‑1 (MQ=255)
                                   cggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGtt                                                                                                           <  2:226403/148‑1 (MQ=255)
                                   cggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGtt                                                                                                           >  1:298433/1‑148 (MQ=255)
                                    ggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTa                                                                                                          >  2:1139060/1‑148 (MQ=255)
                                    ggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTa                                                                                                          <  2:1981807/148‑1 (MQ=255)
                                    ggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTa                                                                                                          <  1:1897977/148‑1 (MQ=255)
                                    ggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTa                                                                                                          >  2:302785/1‑148 (MQ=255)
                                     gtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTAt                                                                                                         <  2:1024689/148‑1 (MQ=255)
                                      tacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATc                                                                                                        <  2:275472/148‑1 (MQ=255)
                                      tacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATc                                                                                                        <  2:777498/148‑1 (MQ=255)
                                       acggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCa                                                                                                       >  2:1019654/1‑148 (MQ=255)
                                       acggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCa                                                                                                       >  2:3164608/1‑148 (MQ=255)
                                            tGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGc                                                                                                  <  1:1399578/148‑1 (MQ=255)
                                             gAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGcc                                                                                                 <  1:1719338/148‑1 (MQ=255)
                                               aGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTg                                                                                               <  1:227852/148‑1 (MQ=255)
                                               aGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTg                                                                                               <  1:1898001/148‑1 (MQ=255)
                                                    aaGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCaa                                                                                          <  2:1844384/148‑1 (MQ=255)
                                                     agttCGCGTACTGAAAACGGTCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAAt                                                                                         <  2:3017627/145‑1 (MQ=255)
                                                       aTCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGc                                                                                       >  1:2976476/1‑148 (MQ=255)
                                                       aTCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGc                                                                                       >  1:1429628/1‑148 (MQ=255)
                                                       aTCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGc                                                                                       >  1:3093960/1‑148 (MQ=255)
                                                        tCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCa                                                                                      <  1:2773603/148‑1 (MQ=255)
                                                        tCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCa                                                                                      <  2:393615/148‑1 (MQ=255)
                                                          gcgTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACt                                                                                    >  2:3286450/1‑148 (MQ=255)
                                                           cgTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTg                                                                                   <  1:2353308/148‑1 (MQ=255)
                                                              aCTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgat                                                                                <  1:1139060/148‑1 (MQ=255)
                                                              aCTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgat                                                                                <  1:302785/148‑1 (MQ=255)
                                                                 gAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgatgat                                                                             <  2:1289604/148‑1 (MQ=255)
                                                                  aaaaCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGatgatg                                                                            >  2:2957022/1‑148 (MQ=255)
                                                                    aaCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAg                                                                          >  1:448753/1‑148 (MQ=255)
                                                                      cGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGt                                                                         >  1:781068/1‑147 (MQ=255)
                                                                      cGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGt                                                                         <  2:781068/147‑1 (MQ=255)
                                                                         gCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGt                                                                     <  2:101125/148‑1 (MQ=255)
                                                                         gCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGt                                                                     >  1:1580055/1‑148 (MQ=255)
                                                                         gCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCACGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACAAGCATGACCAATGCACTGGATGATGAGTCCGt                                                                     >  2:2104960/1‑148 (MQ=255)
                                                                          cgtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTc                                                                    <  1:665697/148‑1 (MQ=255)
                                                                          cgtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTc                                                                    <  2:2438626/148‑1 (MQ=255)
                                                                            tcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCAt                                                                  <  2:2788658/148‑1 (MQ=255)
                                                                            tcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCAt                                                                  >  1:713300/1‑148 (MQ=255)
                                                                            tcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCAt                                                                  >  1:3117181/1‑148 (MQ=255)
                                                                             cgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATc                                                                 >  2:1041133/1‑148 (MQ=255)
                                                                              gtgtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCg                                                                <  1:78062/148‑1 (MQ=255)
                                                                                gtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAg                                                              >  2:3076794/1‑148 (MQ=255)
                                                                                  tCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCg                                                            >  1:3228241/1‑148 (MQ=255)
                                                                                  tCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAAGGCACTGGATGATGAGTCCGTCATCGAGCg                                                            >  2:86772/1‑148 (MQ=255)
                                                                                     gCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCt                                                         <  2:2060464/148‑1 (MQ=255)
                                                                                     gCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCt                                                         <  1:3072614/148‑1 (MQ=255)
                                                                                         gTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGca                                                     <  1:2078756/148‑1 (MQ=255)
                                                                                          tACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGcac                                                    <  2:2895719/148‑1 (MQ=255)
                                                                                                gAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACAcacca                                              >  2:1772582/1‑148 (MQ=255)
                                                                                                 aaGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACAcaccac                                             <  2:819683/148‑1 (MQ=255)
                                                                                                   ggCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACAccacca                                           >  2:977472/1‑148 (MQ=255)
                                                                                                     cGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGc                                         <  2:632907/148‑1 (MQ=255)
                                                                                                            ttCGGTGAAGAAGAAGATGATATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATg                                  >  2:262333/1‑148 (MQ=255)
                                                                                                            ttCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATg                                  <  1:2459346/148‑1 (MQ=255)
                                                                                                               ggTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAg                               <  1:2395714/148‑1 (MQ=255)
                                                                                                                     gaagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTgg                         <  1:1862871/148‑1 (MQ=255)
                                                                                                                     gaagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTgg                         >  2:625340/1‑148 (MQ=255)
                                                                                                                     gaagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTgg                         >  1:2897953/1‑148 (MQ=255)
                                                                                                                      aagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGa                        >  1:121362/1‑148 (MQ=255)
                                                                                                                       agaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGaa                       >  1:1938301/1‑148 (MQ=255)
                                                                                                                         aagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAAct                     >  1:280900/1‑148 (MQ=255)
                                                                                                                         aagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAAct                     >  1:602101/1‑148 (MQ=255)
                                                                                                                          agaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActg                    <  2:1075926/148‑1 (MQ=255)
                                                                                                                           gaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgc                   <  1:486525/148‑1 (MQ=255)
                                                                                                                             tGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgctg                 >  1:2107714/1‑148 (MQ=255)
                                                                                                                               aCATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGc               <  1:1574907/148‑1 (MQ=255)
                                                                                                                                cATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCa              <  1:1262896/148‑1 (MQ=255)
                                                                                                                                cATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCa              >  1:2677324/1‑148 (MQ=255)
                                                                                                                                   tGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGt           >  2:1944606/1‑148 (MQ=255)
                                                                                                                                    gCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTc          >  1:3277224/1‑148 (MQ=255)
                                                                                                                                     cccGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCg         <  1:2220534/148‑1 (MQ=255)
                                                                                                                                         tCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgtaag     >  2:2160514/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAA  >  CP009273/3767267‑3767554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGACCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAA  >  CP009273/3767261‑3767559
                                                                                                                                                     |                                                                                                                                                     
CTGACCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTT                                                                                                                                                       <  SRR19973070.1268999/150‑1 (MQ=60)
 TGACCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTA                                                                                                                                                      <  SRR19973070.1944604/150‑1 (MQ=60)
   ACCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATT                                                                                                                                                    <  SRR19973070.3200602/150‑1 (MQ=60)
     CCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGG                                                                                                                                                  >  SRR19973070.970878/1‑150 (MQ=60)
      CCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGT                                                                                                                                                 <  SRR19973070.2829499/150‑1 (MQ=60)
          CTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTG                                                                                                                                             <  SRR19973070.2608700/150‑1 (MQ=60)
          CTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGAGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTG                                                                                                                                             <  SRR19973070.1248748/150‑1 (MQ=60)
             ATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTG                                                                                                                                          >  SRR19973070.1506400/1‑150 (MQ=60)
               CTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCC                                                                                                                                        >  SRR19973070.541535/1‑150 (MQ=60)
                TGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAGCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCC                                                                                                                                       >  SRR19973070.2295609/1‑150 (MQ=60)
                TGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCC                                                                                                                                       >  SRR19973070.2294782/1‑150 (MQ=60)
                 GGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCG                                                                                                                                      >  SRR19973070.1273921/1‑150 (MQ=60)
                  GGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTTCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGT                                                                                                                                     >  SRR19973070.3235803/1‑150 (MQ=60)
                      AGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACA                                                                                                                                 >  SRR19973070.2045780/1‑150 (MQ=60)
                         CTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACG                                                                                                                              >  SRR19973070.1930261/1‑150 (MQ=60)
                             CGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCA                                                                                                                          <  SRR19973070.2571976/150‑1 (MQ=60)
                             CGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCA                                                                                                                          <  SRR19973070.905800/150‑1 (MQ=60)
                             CGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCA                                                                                                                          >  SRR19973070.377785/1‑150 (MQ=60)
                              GCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACACGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCAC                                                                                                                         <  SRR19973070.1261819/150‑1 (MQ=60)
                              GCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACACGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCAC                                                                                                                         <  SRR19973070.1261713/150‑1 (MQ=60)
                                 GTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATT                                                                                                                      <  SRR19973070.273455/150‑1 (MQ=60)
                                    CCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAG                                                                                                                   <  SRR19973070.3123458/150‑1 (MQ=60)
                                       CACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTT                                                                                                                >  SRR19973070.300506/1‑150 (MQ=60)
                                       CACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTT                                                                                                                >  SRR19973070.300298/1‑150 (MQ=60)
                                       CACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTT                                                                                                                >  SRR19973070.102092/1‑150 (MQ=60)
                                          GGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATC                                                                                                             <  SRR19973070.1908649/150‑1 (MQ=60)
                                          GGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATC                                                                                                             <  SRR19973070.1910834/150‑1 (MQ=60)
                                          GGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATC                                                                                                             <  SRR19973070.119411/150‑1 (MQ=60)
                                             ACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACC                                                                                                          <  SRR19973070.1729100/150‑1 (MQ=60)
                                                  TGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCT                                                                                                     <  SRR19973070.1409012/150‑1 (MQ=60)
                                                  TGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCT                                                                                                     <  SRR19973070.1409017/150‑1 (MQ=60)
                                                   GAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTG                                                                                                    <  SRR19973070.1731231/150‑1 (MQ=60)
                                                    AAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGA                                                                                                   >  SRR19973070.971582/1‑150 (MQ=60)
                                                     AGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGAC                                                                                                  <  SRR19973070.229476/150‑1 (MQ=60)
                                                     AGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGAC                                                                                                  <  SRR19973070.1910858/150‑1 (MQ=60)
                                                           AGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGC                                                                                            >  SRR19973070.1438793/1‑150 (MQ=60)
                                                           AGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGC                                                                                            >  SRR19973070.1439496/1‑150 (MQ=60)
                                                           AGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGC                                                                                            >  SRR19973070.3113893/1‑150 (MQ=60)
                                                           AGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGC                                                                                            >  SRR19973070.3113853/1‑150 (MQ=60)
                                                           AGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGC                                                                                            >  SRR19973070.2995582/1‑150 (MQ=60)
                                                           AGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGC                                                                                            >  SRR19973070.2995571/1‑150 (MQ=60)
                                                           AGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGC                                                                                            >  SRR19973070.2337463/1‑150 (MQ=60)
                                                            GATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCA                                                                                           <  SRR19973070.30986/150‑1 (MQ=60)
                                                              TCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACT                                                                                         <  SRR19973070.2791380/150‑1 (MQ=60)
                                                                 CGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGA                                                                                      <  SRR19973070.2368901/150‑1 (MQ=60)
                                                                 CGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGA                                                                                      <  SRR19973070.2369037/150‑1 (MQ=60)
                                                                    ACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGA                                                                                   <  SRR19973070.1146957/150‑1 (MQ=60)
                                                                    ACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGA                                                                                   <  SRR19973070.304915/150‑1 (MQ=60)
                                                                    ACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGA                                                                                   <  SRR19973070.1804332/150‑1 (MQ=60)
                                                                     CTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGAT                                                                                  <  SRR19973070.3147927/150‑1 (MQ=60)
                                                                       GAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGA                                                                                >  SRR19973070.2805612/1‑150 (MQ=60)
                                                                        AAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAG                                                                               >  SRR19973070.451991/1‑150 (MQ=60)
                                                                          AACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTC                                                                             <  SRR19973070.465476/150‑1 (MQ=60)
                                                                          AACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTC                                                                             >  SRR19973070.786519/1‑150 (MQ=60)
                                                                            CGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCG                                                                           >  SRR19973070.1514625/1‑150 (MQ=60)
                                                                             GGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGT                                                                          >  SRR19973070.1591136/1‑150 (MQ=60)
                                                                                CGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCAT                                                                       <  SRR19973070.670434/150‑1 (MQ=60)
                                                                                CGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCAT                                                                       >  SRR19973070.3137293/1‑150 (MQ=60)
                                                                                CGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCAT                                                                       <  SRR19973070.670444/150‑1 (MQ=60)
                                                                                CGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCAT                                                                       >  SRR19973070.718391/1‑150 (MQ=60)
                                                                                 GTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATC                                                                      >  SRR19973070.2212036/1‑150 (MQ=60)
                                                                                    GTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAG                                                                   <  SRR19973070.78887/150‑1 (MQ=60)
                                                                                      GTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCG                                                                 >  SRR19973070.3249092/1‑150 (MQ=60)
                                                                                           GCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGG                                                            <  SRR19973070.3273494/150‑1 (MQ=60)
                                                                                           GCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGG                                                            <  SRR19973070.3092337/150‑1 (MQ=60)
                                                                                             CAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCA                                                          >  SRR19973070.762786/1‑150 (MQ=60)
                                                                                               GTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACA                                                        <  SRR19973070.2092746/150‑1 (MQ=60)
                                                                                                 ACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACA                                                      >  SRR19973070.954083/1‑150 (MQ=60)
                                                                                                   CCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACC                                                    <  SRR19973070.574800/150‑1 (MQ=60)
                                                                                                       AAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCA                                                <  SRR19973070.681055/150‑1 (MQ=60)
                                                                                                            GTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGTTGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTG                                           >  SRR19973070.844258/1‑150 (MQ=60)
                                                                                                                  TTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAA                                     <  SRR19973070.2475411/150‑1 (MQ=60)
                                                                                                                     GGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTG                                  <  SRR19973070.2411527/150‑1 (MQ=60)
                                                                                                                         AAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGG                              >  SRR19973070.2916700/1‑150 (MQ=60)
                                                                                                                         AAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGG                              >  SRR19973070.2916699/1‑150 (MQ=60)
                                                                                                                          AGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGA                             >  SRR19973070.122445/1‑150 (MQ=60)
                                                                                                                           GAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGACGTGCTGGAA                            >  SRR19973070.1951330/1‑150 (MQ=60)
                                                                                                                           GAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAA                            <  SRR19973070.1875609/150‑1 (MQ=60)
                                                                                                                            AAGAAGGTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAAC                           >  SRR19973070.1446139/1‑150 (MQ=60)
                                                                                                                             AGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACT                          >  SRR19973070.606298/1‑150 (MQ=60)
                                                                                                                             AGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACT                          >  SRR19973070.282735/1‑150 (MQ=60)
                                                                                                                                 GATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTG                      <  SRR19973070.489947/150‑1 (MQ=60)
                                                                                                                                 GATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTG                      >  SRR19973070.2121814/1‑150 (MQ=60)
                                                                                                                                 GATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTG                      >  SRR19973070.2121848/1‑150 (MQ=60)
                                                                                                                                    GACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCA                   >  SRR19973070.2694703/1‑150 (MQ=60)
                                                                                                                                     ACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAG                  <  SRR19973070.1585962/150‑1 (MQ=60)
                                                                                                                                      CATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGG                 <  SRR19973070.1271743/150‑1 (MQ=60)
                                                                                                                                        TTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTC               >  SRR19973070.3298283/1‑150 (MQ=60)
                                                                                                                                           CCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTA            <  SRR19973070.2235112/150‑1 (MQ=60)
                                                                                                                                                  GGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATC     <  SRR19973070.2511586/150‑1 (MQ=60)
                                                                                                                                                   GTTATTGGTATTGCTTCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCC    <  SRR19973070.2904851/150‑1 (MQ=60)
                                                                                                                                                   GTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATAGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCC    <  SRR19973070.2119083/150‑1 (MQ=60)
                                                                                                                                                     TATTGGTATTGCTGCCCGTAACAACGAGCACATTCCGGTTATCACCAGCCTGCCCAATGCACTGGATGATGAGGCCGTCATCGAGCGTCTCGCACACACCACCCGCGGGGAGGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTActcgct  >  SRR19973070.2433728/1‑144 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTGACCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAA  >  CP009273/3767261‑3767559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑