Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,767,410 G→T 100% V590V (GTG→GTT)  mtlA → fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,767,4100GT97.6% 644.1 / 6.1 204V590V (GTG→GTT) mtlAfused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base T (103/96);  total (105/99)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 204 total aligned reads displayed.

CGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAAT  >  CP009273/3767268‑3767555
                                                                                                                                              |                                                                                                                                                 
 gACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGt                                                                                                                                             >  1:369875/1‑148 (MQ=255)
 gACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGt                                                                                                                                             >  1:370435/1‑148 (MQ=255)
   cTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTAt                                                                                                                                           <  1:2456672/148‑1 (MQ=255)
   cTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTAt                                                                                                                                           >  2:570804/1‑148 (MQ=255)
   cTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTAt                                                                                                                                           <  1:2907425/148‑1 (MQ=255)
   cTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTAt                                                                                                                                           <  1:1640873/148‑1 (MQ=255)
    ttATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTAtt                                                                                                                                          >  1:206658/1‑148 (MQ=255)
     tATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtg                                                                                                                                         <  2:1839292/148‑1 (MQ=255)
     tATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtg                                                                                                                                         <  1:1416820/148‑1 (MQ=255)
      aTCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtgc                                                                                                                                        >  2:2484425/1‑148 (MQ=255)
          gggTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGcc                                                                                                                                    <  1:329422/148‑1 (MQ=255)
          gggTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGcc                                                                                                                                    <  1:1107126/148‑1 (MQ=255)
           ggTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGccc                                                                                                                                   >  2:1478711/1‑148 (MQ=255)
             tGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGt                                                                                                                                 >  2:2487493/1‑148 (MQ=255)
              gAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTa                                                                                                                                >  2:2754411/1‑148 (MQ=255)
              gAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTa                                                                                                                                >  1:121731/1‑148 (MQ=255)
                 tCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTaaca                                                                                                                             >  1:2356012/1‑148 (MQ=255)
                  cTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTATTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTaacaa                                                                                                                            <  1:642611/148‑1 (MQ=255)
                     tCGCGGTGCCACACGGTACGGTTGAAGCGAAAGTTCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGa                                                                                                                         >  1:760113/1‑148 (MQ=255)
                     tCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGa                                                                                                                         <  1:1130123/148‑1 (MQ=255)
                      cgcgGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAg                                                                                                                        <  1:413534/148‑1 (MQ=255)
                       gcgGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGc                                                                                                                       <  1:2643905/148‑1 (MQ=255)
                          gTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGcaca                                                                                                                    <  1:1735920/148‑1 (MQ=255)
                           tGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACAt                                                                                                                   <  1:589918/148‑1 (MQ=255)
                            gCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACAtt                                                                                                                  <  2:144303/148‑1 (MQ=255)
                             ccACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTc                                                                                                                 <  1:3363252/148‑1 (MQ=255)
                               acacGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAg                                                                                                               >  2:2097929/1‑148 (MQ=255)
                                 acggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGt                                                                                                             >  2:2228454/1‑148 (MQ=255)
                                 acggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGt                                                                                                             <  2:80183/148‑1 (MQ=255)
                                    gtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTAt                                                                                                          <  2:2030441/148‑1 (MQ=255)
                                     tacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATc                                                                                                         >  2:1279052/1‑148 (MQ=255)
                                      acggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCa                                                                                                        >  1:204826/1‑148 (MQ=255)
                                        ggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCAcc                                                                                                      <  2:3103272/148‑1 (MQ=255)
                                          ttGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAg                                                                                                    >  1:242549/1‑148 (MQ=255)
                                           tGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGc                                                                                                   <  2:2720781/148‑1 (MQ=255)
                                           tGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGc                                                                                                   <  2:3156599/148‑1 (MQ=255)
                                              aGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTg                                                                                                <  2:888553/148‑1 (MQ=255)
                                               gCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGa                                                                                               <  2:1927430/148‑1 (MQ=255)
                                                  aaaGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCa                                                                                            <  2:2666120/148‑1 (MQ=255)
                                                   aaGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCaa                                                                                           >  2:3526958/1‑148 (MQ=255)
                                                      aTCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGc                                                                                        <  1:1684541/148‑1 (MQ=255)
                                                       tCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCa                                                                                       <  1:2673660/148‑1 (MQ=255)
                                                             aCTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgat                                                                                 <  1:3475508/148‑1 (MQ=255)
                                                             aCTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgat                                                                                 >  1:2962788/1‑148 (MQ=255)
                                                              cTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCTCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgatg                                                                                >  1:1135109/1‑148 (MQ=255)
                                                               tGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgatga                                                                               <  1:1356466/148‑1 (MQ=255)
                                                                gAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgatgat                                                                              >  2:2867901/1‑148 (MQ=255)
                                                                  aaaCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGNTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGAtgatga                                                                            <  1:1805925/148‑1 (MQ=255)
                                                                    aCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGt                                                                          >  1:468093/1‑148 (MQ=255)
                                                                    aCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGt                                                                          <  2:2510872/148‑1 (MQ=255)
                                                                     cGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTc                                                                         >  2:2391798/1‑148 (MQ=255)
                                                                     cGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTc                                                                         >  2:3154472/1‑148 (MQ=255)
                                                                     cGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTc                                                                         >  1:2383147/1‑148 (MQ=255)
                                                                     cGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTc                                                                         >  1:1133333/1‑148 (MQ=255)
                                                                        gCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGAGGATGAGTCCGt                                                                      >  1:2212484/1‑148 (MQ=255)
                                                                           tcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCAt                                                                   <  2:2599644/148‑1 (MQ=255)
                                                                              tgtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGa                                                                >  2:357368/1‑148 (MQ=255)
                                                                              tgtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGa                                                                <  1:2357761/148‑1 (MQ=255)
                                                                               gtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAg                                                               <  2:1619802/148‑1 (MQ=255)
                                                                               gtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAg                                                               <  2:370435/148‑1 (MQ=255)
                                                                               gtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAg                                                               <  2:369875/148‑1 (MQ=255)
                                                                                           cccGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGcacac                                                   >  1:3222112/1‑148 (MQ=255)
                                                                                           cccGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGcacac                                                   >  1:544350/1‑148 (MQ=255)
                                                                                                  ggCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACAccacca                                            >  1:796129/1‑148 (MQ=255)
                                                                                                  ggCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACAccacca                                            >  2:2351669/1‑148 (MQ=255)
                                                                                                   gCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAg                                           >  2:545397/1‑148 (MQ=255)
                                                                                                   gCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAg                                           >  2:506564/1‑148 (MQ=255)
                                                                                                         gcgtCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTTGCACACACCACCAGCGTGGa                                     <  2:2461327/145‑1 (MQ=255)
                                                                                                          cTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGAt                                    >  2:1806879/1‑148 (MQ=255)
                                                                                                          cTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGAt                                    >  2:3368156/1‑148 (MQ=255)
                                                                                                                tGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTg                              <  1:1892032/148‑1 (MQ=255)
                                                                                                                 gaagaagaaGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGc                             <  1:3131427/148‑1 (MQ=255)
                                                                                                                  aagaagaagATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACTAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCt                            >  2:1942790/1‑148 (MQ=255)
                                                                                                                  aagaagaagATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCt                            <  2:2274465/148‑1 (MQ=255)
                                                                                                                   agaagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTg                           >  2:1562579/1‑148 (MQ=255)
                                                                                                                   agaagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTg                           >  2:3371422/1‑148 (MQ=255)
                                                                                                                     aagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGa                         >  1:2017341/1‑148 (MQ=255)
                                                                                                                     aagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGa                         <  2:1920550/148‑1 (MQ=255)
                                                                                                                     aagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCAACGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGa                         >  2:857045/1‑148 (MQ=255)
                                                                                                                      agaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGaa                        <  2:3081392/148‑1 (MQ=255)
                                                                                                                        aagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAAct                      >  1:1013682/1‑148 (MQ=255)
                                                                                                                        aagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAAct                      >  2:467252/1‑148 (MQ=255)
                                                                                                                         agaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActg                     <  1:2317579/148‑1 (MQ=255)
                                                                                                                         agaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActg                     <  1:2618320/148‑1 (MQ=255)
                                                                                                                          gaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGATCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgc                    >  2:2504755/1‑148 (MQ=255)
                                                                                                                           aTGACAGTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgct                   <  2:48701/148‑1 (MQ=255)
                                                                                                                            tGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgctg                  <  2:1074461/148‑1 (MQ=255)
                                                                                                                            tGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgctg                  >  1:2522640/1‑148 (MQ=255)
                                                                                                                             gACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTgg                 <  1:150617/148‑1 (MQ=255)
                                                                                                                              aCATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGc                >  1:1728342/1‑148 (MQ=255)
                                                                                                                                aTTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAg              <  2:1793271/148‑1 (MQ=255)
                                                                                                                                aTTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAg              >  1:986096/1‑148 (MQ=255)
                                                                                                                                aTTGCCCGTCTGGTTATTGGTAGTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAg              <  2:1859626/148‑1 (MQ=255)
                                                                                                                                  tGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGt            <  2:1348549/148‑1 (MQ=255)
                                                                                                                                  tGCCCGTCTGGTTATTGGTATTGCGGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGt            <  2:3013438/148‑1 (MQ=255)
                                                                                                                                       gTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgtaa       <  1:2820190/148‑1 (MQ=255)
                                                                                                                                         cTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgtaagt     >  1:1065653/1‑148 (MQ=255)
                                                                                                                                           ggTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgtaagtaa   >  2:517704/1‑148 (MQ=255)
                                                                                                                                           ggTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgtaagtaa   >  2:743676/1‑148 (MQ=255)
                                                                                                                                            gTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTaat  >  1:567870/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
CGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAAT  >  CP009273/3767268‑3767555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATC  >  CP009273/3767263‑3767556
                                                                                                                                                   |                                                                                                                                                  
GACCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTAT                                                                                                                                                  <  SRR19973066.3105332/150‑1 (MQ=60)
   CCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGG                                                                                                                                               >  SRR19973066.139602/1‑150 (MQ=60)
    CCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAATATGACATTGCCCGTCTGGTTATTGGT                                                                                                                                              >  SRR19973066.373304/1‑150 (MQ=60)
    CCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGT                                                                                                                                              >  SRR19973066.372773/1‑150 (MQ=60)
    CCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGT                                                                                                                                              >  SRR19973066.373337/1‑150 (MQ=60)
     CGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTA                                                                                                                                             >  SRR19973066.1831482/1‑150 (MQ=60)
       ACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATT                                                                                                                                           >  SRR19973066.208393/1‑150 (MQ=60)
       ACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATT                                                                                                                                           <  SRR19973066.2956194/150‑1 (MQ=60)
        CTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTG                                                                                                                                          <  SRR19973066.2473415/150‑1 (MQ=60)
        CTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTG                                                                                                                                          <  SRR19973066.1137251/150‑1 (MQ=60)
        CTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTG                                                                                                                                          <  SRR19973066.2926836/150‑1 (MQ=60)
        CTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTG                                                                                                                                          >  SRR19973066.2683963/1‑150 (MQ=60)
        CTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTG                                                                                                                                          <  SRR19973066.1652807/150‑1 (MQ=60)
          TATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCT                                                                                                                                        <  SRR19973066.1426970/150‑1 (MQ=60)
               GGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCG                                                                                                                                   <  SRR19973066.630319/150‑1 (MQ=60)
               GGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCG                                                                                                                                   <  SRR19973066.1115143/150‑1 (MQ=60)
               GGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCG                                                                                                                                   <  SRR19973066.331933/150‑1 (MQ=60)
                GGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGT                                                                                                                                  >  SRR19973066.2264674/1‑150 (MQ=60)
                GGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGT                                                                                                                                  >  SRR19973066.460021/1‑150 (MQ=60)
                 GTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTA                                                                                                                                 >  SRR19973066.122959/1‑150 (MQ=60)
                    AGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACA                                                                                                                              >  SRR19973066.2372004/1‑150 (MQ=60)
                    AGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACA                                                                                                                              >  SRR19973066.2372002/1‑150 (MQ=60)
                       CTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACG                                                                                                                           >  SRR19973066.3314007/1‑150 (MQ=60)
                       CTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTATTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACG                                                                                                                           <  SRR19973066.647260/150‑1 (MQ=60)
                        TATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGTTCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGA                                                                                                                          >  SRR19973066.765744/1‑150 (MQ=60)
                         ATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAG                                                                                                                         >  SRR19973066.1806031/1‑150 (MQ=60)
                          TCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGC                                                                                                                        <  SRR19973066.1138413/150‑1 (MQ=60)
                          TCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGC                                                                                                                        <  SRR19973066.1138234/150‑1 (MQ=60)
                           CGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCA                                                                                                                       <  SRR19973066.416765/150‑1 (MQ=60)
                            GCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCAC                                                                                                                      <  SRR19973066.2661550/150‑1 (MQ=60)
                            GCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCAC                                                                                                                      <  SRR19973066.1654526/150‑1 (MQ=60)
                            GCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCAC                                                                                                                      <  SRR19973066.2661677/150‑1 (MQ=60)
                              GGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACAT                                                                                                                    >  SRR19973066.1051376/1‑150 (MQ=60)
                               GTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATT                                                                                                                   <  SRR19973066.1747020/150‑1 (MQ=60)
                               GTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACANT                                                                                                                   <  SRR19973066.1748453/150‑1 (MQ=60)
                                TGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTC                                                                                                                  <  SRR19973066.594023/150‑1 (MQ=60)
                                TGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTC                                                                                                                  <  SRR19973066.2199883/150‑1 (MQ=60)
                                  CCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAG                                                                                                                <  SRR19973066.3385674/150‑1 (MQ=60)
                                         GTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCA                                                                                                         <  SRR19973066.513505/150‑1 (MQ=60)
                                         GTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCA                                                                                                         >  SRR19973066.206541/1‑150 (MQ=60)
                                         GTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCA                                                                                                         >  SRR19973066.206555/1‑150 (MQ=60)
                                             GGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAG                                                                                                     >  SRR19973066.244444/1‑150 (MQ=60)
                                             GGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAG                                                                                                     <  SRR19973066.791750/150‑1 (MQ=60)
                                             GGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAG                                                                                                     <  SRR19973066.1194398/150‑1 (MQ=60)
                                              GTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATAACCAGC                                                                                                    >  SRR19973066.382864/1‑150 (MQ=60)
                                                TGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCT                                                                                                  >  SRR19973066.557403/1‑150 (MQ=60)
                                                           ATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCAC                                                                                       <  SRR19973066.1696810/150‑1 (MQ=60)
                                                           ATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCAC                                                                                       <  SRR19973066.236315/150‑1 (MQ=60)
                                                            TCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACT                                                                                      <  SRR19973066.2691522/150‑1 (MQ=60)
                                                                GTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGAT                                                                                  >  SRR19973066.2982402/1‑150 (MQ=60)
                                                                GTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGAT                                                                                  <  SRR19973066.574838/150‑1 (MQ=60)
                                                                GTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGAT                                                                                  <  SRR19973066.284932/150‑1 (MQ=60)
                                                                 TACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCTCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATG                                                                                 >  SRR19973066.1143244/1‑150 (MQ=60)
                                                                  ACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGA                                                                                <  SRR19973066.3498712/150‑1 (MQ=60)
                                                                   CTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGAT                                                                               <  SRR19973066.923145/150‑1 (MQ=60)
                                                                    TGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATG                                                                              <  SRR19973066.1366287/150‑1 (MQ=60)
                                                                     GAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCCCTGGATGATGA                                                                             >  SRR19973066.1592050/1‑150 (MQ=60)
                                                                      AAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAG                                                                            >  SRR19973066.3506287/1‑150 (MQ=60)
                                                                       AAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGNTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGT                                                                           <  SRR19973066.1818749/150‑1 (MQ=60)
                                                                       AAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGT                                                                           >  SRR19973066.471654/1‑150 (MQ=60)
                                                                        AACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTC                                                                          >  SRR19973066.1141457/1‑150 (MQ=60)
                                                                        AACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTC                                                                          >  SRR19973066.2399215/1‑150 (MQ=60)
                                                                           GGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGAGGATGAGTCCGT                                                                       >  SRR19973066.2227804/1‑150 (MQ=60)
                                                                                 CGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGA                                                                 >  SRR19973066.2025882/1‑150 (MQ=60)
                                                                                  GTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAG                                                                <  SRR19973066.246343/150‑1 (MQ=60)
                                                                                   TGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGC                                                               <  SRR19973066.2373753/150‑1 (MQ=60)
                                                                                              TACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACAC                                                    >  SRR19973066.3243425/1‑150 (MQ=60)
                                                                                              TACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACAC                                                    >  SRR19973066.548282/1‑150 (MQ=60)
                                                                                                    GAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACC                                              >  SRR19973066.2377559/1‑150 (MQ=60)
                                                                                                     AAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCA                                             >  SRR19973066.801976/1‑150 (MQ=60)
                                                                                                         CGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGT                                         <  SRR19973066.2506348/150‑1 (MQ=60)
                                                                                                          GTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTG                                        <  SRR19973066.1846376/150‑1 (MQ=60)
                                                                                                          GTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATAAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTG                                        <  SRR19973066.1241514/150‑1 (MQ=60)
                                                                                                             CGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGAT                                     <  SRR19973066.1739757/150‑1 (MQ=60)
                                                                                                                 TCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAG                                 <  SRR19973066.3319215/150‑1 (MQ=60)
                                                                                                                   GGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTG                               >  SRR19973066.2988802/1‑150 (MQ=60)
                                                                                                                   GGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTG                               >  SRR19973066.1104844/1‑150 (MQ=60)
                                                                                                                     TGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCT                             <  SRR19973066.1905520/150‑1 (MQ=60)
                                                                                                                      GAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTG                            <  SRR19973066.3152330/150‑1 (MQ=60)
                                                                                                                        AGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGA                          >  SRR19973066.2031464/1‑150 (MQ=60)
                                                                                                                           AGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACT                       >  SRR19973066.2458133/1‑150 (MQ=60)
                                                                                                                           AGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACT                       >  SRR19973066.3404962/1‑150 (MQ=60)
                                                                                                                           AGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACT                       >  SRR19973066.1021144/1‑150 (MQ=60)
                                                                                                                              AGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCT                    <  SRR19973066.2635998/150‑1 (MQ=60)
                                                                                                                              AGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCT                    <  SRR19973066.2635501/150‑1 (MQ=60)
                                                                                                                              AGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCT                    <  SRR19973066.2333426/150‑1 (MQ=60)
                                                                                                                              AGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCT                    <  SRR19973066.2067103/150‑1 (MQ=60)
                                                                                                                               GATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTG                   >  SRR19973066.2539796/1‑150 (MQ=60)
                                                                                                                                 TGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGC                 >  SRR19973066.1740836/1‑150 (MQ=60)
                                                                                                                                  GACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCA                <  SRR19973066.1489111/150‑1 (MQ=60)
                                                                                                                                  GACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCA                <  SRR19973066.152032/150‑1 (MQ=60)
                                                                                                                                  GACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCA                >  SRR19973066.2418506/1‑150 (MQ=60)
                                                                                                                                   ACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAG               >  SRR19973066.993367/1‑150 (MQ=60)
                                                                                                                                           CGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAG       <  SRR19973066.3243162/150‑1 (MQ=60)
                                                                                                                                            GTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTACGT      >  SRR19973066.1142992/1‑150 (MQ=60)
                                                                                                                                            GTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGT      >  SRR19973066.687075/1‑150 (MQ=60)
                                                                                                                                            GTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGT      <  SRR19973066.2837967/150‑1 (MQ=60)
                                                                                                                                            GTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGT      <  SRR19973066.2839142/150‑1 (MQ=60)
                                                                                                                                            GTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGT      >  SRR19973066.1073466/1‑150 (MQ=60)
                                                                                                                                               TGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAAT   >  SRR19973066.571890/1‑150 (MQ=60)
                                                                                                                                                GGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATC  >  SRR19973066.1904789/1‑150 (MQ=60)
                                                                                                                                                GGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATC  >  SRR19973066.695237/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                  
GACCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATC  >  CP009273/3767263‑3767556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑