Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,178,969 G→T 91.9% R1231L (CGT→CTT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,178,9690GT91.9% 453.0 / 32.0 172R1231L (CGT→CTT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (7/7);  new base T (50/108);  total (57/115)
Fisher's exact test for biased strand distribution p-value = 2.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 177 total aligned reads displayed.

AGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGT  >  CP009273/4178824‑4179116
                                                                                                                                                 |                                                                                                                                                   
aGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCttt                                                                                                                                                   <  2:1515934/148‑1 (MQ=255)
aGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCttt                                                                                                                                                   <  2:1834404/148‑1 (MQ=255)
  cGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTAc                                                                                                                                                 >  1:3084739/1‑148 (MQ=255)
   gATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACa                                                                                                                                                <  2:2549168/148‑1 (MQ=255)
    aTCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACAt                                                                                                                                               <  2:2313502/148‑1 (MQ=255)
    aTCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACAt                                                                                                                                               <  2:2313513/148‑1 (MQ=255)
     tCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATc                                                                                                                                              >  1:1846785/1‑148 (MQ=255)
      ccGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCg                                                                                                                                             <  2:1532463/148‑1 (MQ=255)
      ccGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCg                                                                                                                                             <  2:1180384/148‑1 (MQ=255)
       cGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTANTTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGt                                                                                                                                            <  1:1676652/148‑1 (MQ=255)
       cGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTANTTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGt                                                                                                                                            <  1:1676665/148‑1 (MQ=255)
               gagaTGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGACGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAg                                                                                                                                    >  2:2070063/1‑148 (MQ=255)
               gagaTGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAg                                                                                                                                    <  1:1219159/148‑1 (MQ=255)
                  atgatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTAc                                                                                                                                 <  1:308923/148‑1 (MQ=255)
                     atTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAgg                                                                                                                              >  2:433485/1‑148 (MQ=255)
                      ttCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATTCTGTTACTCTTTACATCGTTAACGAAGTACAGGa                                                                                                                             >  2:3268449/1‑148 (MQ=255)
                      ttCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGa                                                                                                                             <  1:2081537/148‑1 (MQ=255)
                        ccGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACg                                                                                                                           <  1:3170900/148‑1 (MQ=255)
                           aaaTGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGtat                                                                                                                        >  2:1767164/1‑148 (MQ=255)
                             aTGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATAc                                                                                                                      >  2:599543/1‑148 (MQ=255)
                             aTGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATAc                                                                                                                      >  1:3157778/1‑148 (MQ=255)
                              tGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGGGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATAcc                                                                                                                     >  2:35607/1‑148 (MQ=255)
                                  gTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCt                                                                                                                 <  1:8817/148‑1 (MQ=255)
                                     aGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCa                                                                                                              >  1:800008/1‑148 (MQ=255)
                                     aGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCa                                                                                                              <  2:766682/148‑1 (MQ=255)
                                       cTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAgg                                                                                                            >  2:1309914/1‑148 (MQ=255)
                                         cAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGc                                                                                                          >  1:898493/1‑148 (MQ=255)
                                          aaCGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCg                                                                                                         >  2:679721/1‑148 (MQ=255)
                                             gtgtTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTa                                                                                                      <  1:2773183/148‑1 (MQ=255)
                                             gtgtTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTa                                                                                                      <  1:1527344/148‑1 (MQ=255)
                                               gtTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAg                                                                                                    <  1:1311962/148‑1 (MQ=255)
                                               gtTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAg                                                                                                    <  1:642721/148‑1 (MQ=255)
                                                ttCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGa                                                                                                   <  2:2334854/148‑1 (MQ=255)
                                                 tCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGAt                                                                                                  <  2:1796882/148‑1 (MQ=255)
                                                 tCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGAt                                                                                                  >  1:1718019/1‑148 (MQ=255)
                                                   gAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTa                                                                                                >  1:379890/1‑148 (MQ=255)
                                                    aaGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTaa                                                                                               <  1:2765186/148‑1 (MQ=255)
                                                    aaGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTaa                                                                                               <  2:1670859/148‑1 (MQ=255)
                                                      ggTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAggg                                                                                                           >  1:2992643/1‑134 (MQ=255)
                                                      ggTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACg                                                                                             <  1:284148/148‑1 (MQ=255)
                                                       gTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGa                                                                                            <  1:249908/148‑1 (MQ=255)
                                                            cGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAac                                                                                       >  1:1565850/1‑148 (MQ=255)
                                                             gtgtAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAaca                                                                                      <  1:3039308/148‑1 (MQ=255)
                                                             gtgtAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAaca                                                                                      <  1:1609170/148‑1 (MQ=255)
                                                              tgtAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAacac                                                                                     <  1:738274/148‑1 (MQ=255)
                                                                tAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACAt                                                                                   <  1:2557104/148‑1 (MQ=255)
                                                                  gAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCg                                                                                 <  2:1646339/148‑1 (MQ=255)
                                                                   aaCGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGa                                                                                <  1:2077460/148‑1 (MQ=255)
                                                                   aaCGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGa                                                                                <  2:2201439/148‑1 (MQ=255)
                                                                   aaCGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGa                                                                                <  2:887618/148‑1 (MQ=255)
                                                                    aCGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGaa                                                                               <  1:2270407/148‑1 (MQ=255)
                                                                    aCGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGaa                                                                               <  2:304640/148‑1 (MQ=255)
                                                                     cGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAg                                                                              <  1:459797/148‑1 (MQ=255)
                                                                      gtggtgACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGt                                                                             <  2:3006627/148‑1 (MQ=255)
                                                                      gtggtgACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGt                                                                             <  1:191073/148‑1 (MQ=255)
                                                                       tggtgACGTAATTTCCGACGGTCTGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGtt                                                                            <  2:167793/148‑1 (MQ=255)
                                                                       tggtgACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGtt                                                                            <  2:417141/148‑1 (MQ=255)
                                                                            aCGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTAtcgt                                                                       <  2:2586170/148‑1 (MQ=255)
                                                                            aCGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTAtcgt                                                                       <  2:1645403/148‑1 (MQ=255)
                                                                             cGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTAtcgtt                                                                      <  1:2375720/148‑1 (MQ=255)
                                                                                aaTTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTAtcgttcgt                                                                   <  1:588761/148‑1 (MQ=255)
                                                                                   ttCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAg                                                                >  1:464505/1‑148 (MQ=255)
                                                                                        aCGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGAtgct                                                           >  1:2067467/1‑148 (MQ=255)
                                                                                            tCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGt                                                       >  1:1105719/1‑148 (MQ=255)
                                                                                                 aaGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGc                                                  <  2:738478/148‑1 (MQ=255)
                                                                                                    cgccgcACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTAc                                               >  2:455209/1‑148 (MQ=255)
                                                                                                       cgcACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCAt                                            <  2:1914940/148‑1 (MQ=255)
                                                                                                            gACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTa                                       <  2:1527742/148‑1 (MQ=255)
                                                                                                            gACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTa                                       <  1:2986677/148‑1 (MQ=255)
                                                                                                             aCATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTaa                                      <  1:1511267/148‑1 (MQ=255)
                                                                                                               aTTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTCTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAcg                                    <  2:3043257/148‑1 (MQ=255)
                                                                                                                 tctgcgtctgcgTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAcgcg                                  <  1:2729728/148‑1 (MQ=255)
                                                                                                                 tctgcgtctgcgTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAcgcg                                  <  1:500911/148‑1 (MQ=255)
                                                                                                                  ctgcgtctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCgg                                 <  1:2499883/148‑1 (MQ=255)
                                                                                                                   tgcgtctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCggg                                <  2:864156/148‑1 (MQ=255)
                                                                                                                   tgcgtctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCggg                                >  2:2983794/1‑148 (MQ=255)
                                                                                                                    gcgtctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGt                               <  1:599543/148‑1 (MQ=255)
                                                                                                                        ctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCt                           >  1:61870/1‑148 (MQ=255)
                                                                                                                            gtggtgTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGa                       <  2:1458474/148‑1 (MQ=255)
                                                                                                                             tggtgTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGAc                      <  2:1283200/148‑1 (MQ=255)
                                                                                                                             tggtgTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGAc                      <  1:1132891/148‑1 (MQ=255)
                                                                                                                             tggtgTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGAc                      >  2:1231919/1‑148 (MQ=255)
                                                                                                                               gtggTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGTAGTATACCGTCTGCAGGGCGTTAAGTTTAAAGATAATCACATCGAAGTTATCGTTCGTCAGTTGCTGCGTTCAGCTACCATCGTTAACGCGGGTAGCTCAGACtt                    <  1:977132/148‑1 (MQ=255)
                                                                                                                                tgtTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTc                   <  1:3107939/148‑1 (MQ=255)
                                                                                                                                tgtTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTc                   <  1:679721/148‑1 (MQ=255)
                                                                                                                                  ttCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCt                 <  2:2838338/148‑1 (MQ=255)
                                                                                                                                   tCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTg                >  1:1047173/1‑148 (MQ=255)
                                                                                                                                   tCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTg                >  1:1145511/1‑148 (MQ=255)
                                                                                                                                   tCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTg                <  1:103451/148‑1 (MQ=255)
                                                                                                                                      tGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGaa             <  1:2093527/148‑1 (MQ=255)
                                                                                                                                       gCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAg            >  1:3296113/1‑148 (MQ=255)
                                                                                                                                           ttACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGa        >  2:1517233/1‑148 (MQ=255)
                                                                                                                                           ttACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGa        >  1:2068905/1‑148 (MQ=255)
                                                                                                                                           ttACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGa        >  1:1127265/1‑148 (MQ=255)
                                                                                                                                            tACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGACGGCGaa       >  2:2006763/1‑148 (MQ=255)
                                                                                                                                            tACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGaa       >  1:676234/1‑148 (MQ=255)
                                                                                                                                            tACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGaa       >  1:159187/1‑148 (MQ=255)
                                                                                                                                              ctctTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACa     >  2:1032302/1‑148 (MQ=255)
                                                                                                                                               tCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAg    >  2:310137/1‑148 (MQ=255)
                                                                                                                                                 gTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGt  <  1:1759999/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGT  >  CP009273/4178824‑4179116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTG  >  CP009273/4178821‑4179118
                                                                                                                                                    |                                                                                                                                                     
GGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTT                                                                                                                                                      <  SRR19973070.3006474/150‑1 (MQ=60)
GGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTT                                                                                                                                                      <  SRR19973070.738583/150‑1 (MQ=60)
GGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTT                                                                                                                                                      >  SRR19973070.1538475/1‑150 (MQ=60)
GGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTT                                                                                                                                                      >  SRR19973070.1538472/1‑150 (MQ=60)
  TAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTA                                                                                                                                                    >  SRR19973070.1751238/1‑150 (MQ=60)
   AGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTAC                                                                                                                                                   >  SRR19973070.3104578/1‑150 (MQ=60)
    GCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACA                                                                                                                                                  <  SRR19973070.495866/150‑1 (MQ=60)
    GCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACA                                                                                                                                                  <  SRR19973070.1514807/150‑1 (MQ=60)
      GATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATC                                                                                                                                                >  SRR19973070.1857913/1‑150 (MQ=60)
      GATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATC                                                                                                                                                >  SRR19973070.1859451/1‑150 (MQ=60)
       ATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCG                                                                                                                                               <  SRR19973070.2466343/150‑1 (MQ=60)
          CGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTANTTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTA                                                                                                                                            <  SRR19973070.1688139/150‑1 (MQ=60)
          CGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTANTTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTA                                                                                                                                            <  SRR19973070.1688126/150‑1 (MQ=60)
                AAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAG                                                                                                                                      >  SRR19973070.2089521/1‑150 (MQ=60)
                  GAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTA                                                                                                                                    <  SRR19973070.1227703/150‑1 (MQ=60)
                     ATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAG                                                                                                                                 <  SRR19973070.311120/150‑1 (MQ=60)
                     ATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAG                                                                                                                                 <  SRR19973070.2101463/150‑1 (MQ=60)
                      TGATTCCGAAATGGCGTCAGCTCAACTTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGG                                                                                                                                <  SRR19973070.873493/150‑1 (MQ=60)
                         TTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACG                                                                                                                             <  SRR19973070.2095541/150‑1 (MQ=60)
                           CCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTA                                                                                                                           <  SRR19973070.3191425/150‑1 (MQ=60)
                              AAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATAC                                                                                                                        >  SRR19973070.3178207/1‑150 (MQ=60)
                                 TGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGT                                                                                                                     >  SRR19973070.1927869/1‑150 (MQ=60)
                                    CGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTG                                                                                                                  >  SRR19973070.2253977/1‑150 (MQ=60)
                                     GTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGC                                                                                                                 <  SRR19973070.732360/150‑1 (MQ=60)
                                     GTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGC                                                                                                                 <  SRR19973070.8948/150‑1 (MQ=60)
                                     GTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGC                                                                                                                 <  SRR19973070.11339/150‑1 (MQ=60)
                                      TCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCA                                                                                                                >  SRR19973070.805524/1‑150 (MQ=60)
                                       CAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAG                                                                                                               <  SRR19973070.1664060/150‑1 (MQ=60)
                                          CTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGC                                                                                                            >  SRR19973070.904855/1‑150 (MQ=60)
                                          CTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGC                                                                                                            >  SRR19973070.904851/1‑150 (MQ=60)
                                                GTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAG                                                                                                      <  SRR19973070.2790955/150‑1 (MQ=60)
                                                GTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAG                                                                                                      <  SRR19973070.1538077/150‑1 (MQ=60)
                                                 TGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGA                                                                                                     <  SRR19973070.941023/150‑1 (MQ=60)
                                                  GTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGAT                                                                                                    >  SRR19973070.1729897/1‑150 (MQ=60)
                                                  GTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGAT                                                                                                    <  SRR19973070.647307/150‑1 (MQ=60)
                                                  GTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGAT                                                                                                    <  SRR19973070.682901/150‑1 (MQ=60)
                                                  GTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGAT                                                                                                    <  SRR19973070.1321060/150‑1 (MQ=60)
                                                    TCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTA                                                                                                  >  SRR19973070.382674/1‑150 (MQ=60)
                                                      GAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAAC                                                                                                <  SRR19973070.311116/150‑1 (MQ=60)
                                                       AAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGagatcggaagag                                                                                               >  SRR19973070.3011811/1‑138 (MQ=60)
                                                       AAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACG                                                                                               <  SRR19973070.2782898/150‑1 (MQ=60)
                                                         GGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGAT                                                                                             <  SRR19973070.286036/150‑1 (MQ=60)
                                                          GTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATA                                                                                            <  SRR19973070.251615/150‑1 (MQ=60)
                                                             AACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAAC                                                                                         >  SRR19973070.1576561/1‑150 (MQ=60)
                                                             AACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAAC                                                                                         >  SRR19973070.1576865/1‑150 (MQ=60)
                                                             AACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAAC                                                                                         >  SRR19973070.1576869/1‑150 (MQ=60)
                                                             AACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAAC                                                                                         >  SRR19973070.2814212/1‑150 (MQ=60)
                                                                GTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACA                                                                                      <  SRR19973070.3058678/150‑1 (MQ=60)
                                                                GTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACA                                                                                      <  SRR19973070.3058631/150‑1 (MQ=60)
                                                                GTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACA                                                                                      <  SRR19973070.1620383/150‑1 (MQ=60)
                                                                GTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACA                                                                                      <  SRR19973070.1620254/150‑1 (MQ=60)
                                                                GTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACA                                                                                      <  SRR19973070.1207824/150‑1 (MQ=60)
                                                                 TGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACAT                                                                                     <  SRR19973070.743499/150‑1 (MQ=60)
                                                                   TAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCG                                                                                   <  SRR19973070.2573812/150‑1 (MQ=60)
                                                                      AACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAG                                                                                >  SRR19973070.595202/1‑150 (MQ=60)
                                                                      AACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAG                                                                                <  SRR19973070.2091444/150‑1 (MQ=60)
                                                                       ACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGG                                                                               <  SRR19973070.2288742/150‑1 (MQ=60)
                                                                       ACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGG                                                                               <  SRR19973070.2285486/150‑1 (MQ=60)
                                                                        CGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTT                                                                              <  SRR19973070.463082/150‑1 (MQ=60)
                                                                        CGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTT                                                                              <  SRR19973070.436636/150‑1 (MQ=60)
                                                                        CGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGAAATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTT                                                                              <  SRR19973070.2529623/150‑1 (MQ=60)
                                                                         GTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTA                                                                             <  SRR19973070.192544/150‑1 (MQ=60)
                                                                            GTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCG                                                                          >  SRR19973070.1292159/1‑150 (MQ=60)
                                                                                CGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCG                                                                      <  SRR19973070.2391424/150‑1 (MQ=60)
                                                                                   AATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCA                                                                   <  SRR19973070.592828/150‑1 (MQ=60)
                                                                                    ATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAG                                                                  >  SRR19973070.467818/1‑150 (MQ=60)
                                                                                       TCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATG                                                               <  SRR19973070.1100080/150‑1 (MQ=60)
                                                                                       TCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATG                                                               <  SRR19973070.3114888/150‑1 (MQ=60)
                                                                                         CGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCT                                                             >  SRR19973070.2081402/1‑150 (MQ=60)
                                                                                             GGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGT                                                         >  SRR19973070.1113299/1‑150 (MQ=60)
                                                                                               TCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAA                                                       <  SRR19973070.2753441/150‑1 (MQ=60)
                                                                                                            CACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTT                                          >  SRR19973070.3196644/1‑150 (MQ=60)
                                                                                                             ACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTA                                         <  SRR19973070.560031/150‑1 (MQ=60)
                                                                                                               GACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAC                                       <  SRR19973070.3005822/150‑1 (MQ=60)
                                                                                                                ACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACG                                      <  SRR19973070.1521837/150‑1 (MQ=60)
                                                                                                                ACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTGTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACG                                      <  SRR19973070.3172876/150‑1 (MQ=60)
                                                                                                                  ATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCG                                    >  SRR19973070.301058/1‑150 (MQ=60)
                                                                                                                    TCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGG                                  <  SRR19973070.2747324/150‑1 (MQ=60)
                                                                                                                    TCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGG                                  <  SRR19973070.504390/150‑1 (MQ=60)
                                                                                                                     CTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGT                                 <  SRR19973070.2516105/150‑1 (MQ=60)
                                                                                                                       GCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAG                               <  SRR19973070.603718/150‑1 (MQ=60)
                                                                                                                         GTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCT                             >  SRR19973070.62567/1‑150 (MQ=60)
                                                                                                                         GTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCT                             >  SRR19973070.62878/1‑150 (MQ=60)
                                                                                                                                TGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTT                      <  SRR19973070.2996457/150‑1 (MQ=60)
                                                                                                                                TGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTT                      <  SRR19973070.1140723/150‑1 (MQ=60)
                                                                                                                                 GGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTC                     >  SRR19973070.2642704/1‑150 (MQ=60)
                                                                                                                                  GTGGTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGTAGTATACCGTCTGCAGGGCGTTAAGTTTAAAGATAATCACATCGAAGTTATCGTTCGTCAGTTGCTGCGTTCAGCTACCATCGTTAACGCGGGTAGCTCAGACTTCC                    <  SRR19973070.984110/150‑1 (MQ=60)
                                                                                                                                   TGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCT                   <  SRR19973070.334377/150‑1 (MQ=60)
                                                                                                                                   TGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCT                   <  SRR19973070.3127987/150‑1 (MQ=60)
                                                                                                                                   TGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCT                   <  SRR19973070.684570/150‑1 (MQ=60)
                                                                                                                                    GTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTG                  >  SRR19973070.1054549/1‑150 (MQ=60)
                                                                                                                                    GTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTG                  >  SRR19973070.1153471/1‑150 (MQ=60)
                                                                                                                                      TCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGA                <  SRR19973070.104432/150‑1 (MQ=60)
                                                                                                                                        ATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAG              >  SRR19973070.3317242/1‑150 (MQ=60)
                                                                                                                                        ATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAG              <  SRR19973070.1723114/150‑1 (MQ=60)
                                                                                                                                         TGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGG             <  SRR19973070.2107603/150‑1 (MQ=60)
                                                                                                                                            TGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGA          >  SRR19973070.2082843/1‑150 (MQ=60)
                                                                                                                                            TGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGA          >  SRR19973070.2082752/1‑150 (MQ=60)
                                                                                                                                            TGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGA          >  SRR19973070.1135831/1‑150 (MQ=60)
                                                                                                                                            TGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGA          >  SRR19973070.1135024/1‑150 (MQ=60)
                                                                                                                                             GTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAA         >  SRR19973070.679505/1‑150 (MQ=60)
                                                                                                                                             GTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAA         >  SRR19973070.681057/1‑150 (MQ=60)
                                                                                                                                             GTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAA         >  SRR19973070.160506/1‑150 (MQ=60)
                                                                                                                                                ACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAG      >  SRR19973070.1836541/1‑150 (MQ=60)
                                                                                                                                                 CTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGG     >  SRR19973070.2542478/1‑150 (MQ=60)
                                                                                                                                                   CTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTT   <  SRR19973070.1894999/150‑1 (MQ=60)
                                                                                                                                                    GTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTG  <  SRR19973070.1772183/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTG  >  CP009273/4178821‑4179118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑