Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,178,969 G→T 100% R1231L (CGT→CTT)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,178,9690GT100.0% 646.5 / NA 201R1231L (CGT→CTT) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (77/124);  total (77/124)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 202 total aligned reads displayed.

GCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACA  >  CP009273/4178825‑4179113
                                                                                                                                                |                                                                                                                                                
gCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGGTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTa                                                                                                                                               >  2:1332680/1‑148 (MQ=255)
 cGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGACCGTGGTGNCGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTAc                                                                                                                                              <  1:1785686/148‑1 (MQ=255)
   aTCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACAt                                                                                                                                            >  2:663521/1‑148 (MQ=255)
   aTCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACAt                                                                                                                                            <  1:3048958/148‑1 (MQ=255)
     ccGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCg                                                                                                                                          >  2:1814332/1‑148 (MQ=255)
     ccGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCg                                                                                                                                          <  2:1981604/148‑1 (MQ=255)
       gTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGtt                                                                                                                                        >  1:590021/1‑148 (MQ=255)
        tACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTa                                                                                                                                       >  2:2950998/1‑148 (MQ=255)
         aCGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTaa                                                                                                                                      >  2:1991234/1‑148 (MQ=255)
         aCGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTaa                                                                                                                                      >  2:217024/1‑148 (MQ=255)
           gAAGAGATGATTCCGAATTGGCGTCAGCTCATCGTGTTAGAAGGTGAACGTGTTGATCGTGGTGACGTAATTTCGGACGGTCCGGAAGCTCCGAACGAAATTCTTCGTCTGCGTGGTGTTCATGCTGTTACTATTTACATCGTTAACg                                                                                                                                    <  1:362635/148‑1 (MQ=255)
            aaGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGa                                                                                                                                   <  1:1456743/148‑1 (MQ=255)
             agagATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGaa                                                                                                                                  <  2:2620712/148‑1 (MQ=255)
              gagaTGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAg                                                                                                                                 <  2:1119188/148‑1 (MQ=255)
               agaTGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGt                                                                                                                                >  2:1317505/1‑148 (MQ=255)
                gatgatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTa                                                                                                                               <  2:2548529/148‑1 (MQ=255)
                 atgatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTAc                                                                                                                              <  2:2189416/148‑1 (MQ=255)
                 atgatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTAc                                                                                                                              <  1:596589/148‑1 (MQ=255)
                 atgatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTAc                                                                                                                              <  1:139820/148‑1 (MQ=255)
                  tgatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACa                                                                                                                             <  2:1190263/148‑1 (MQ=255)
                  tgatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACa                                                                                                                             <  1:826968/148‑1 (MQ=255)
                   gatTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAg                                                                                                                            >  2:2548906/1‑148 (MQ=255)
                    atTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAgg                                                                                                                           >  1:1310371/1‑148 (MQ=255)
                       ccGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACg                                                                                                                        <  1:3144679/148‑1 (MQ=255)
                        cGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGt                                                                                                                       <  2:849610/148‑1 (MQ=255)
                            aTGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATAc                                                                                                                   >  2:2778626/1‑148 (MQ=255)
                            aTGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATAc                                                                                                                   >  1:1506400/1‑148 (MQ=255)
                             tGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATAcc                                                                                                                  >  2:2273709/1‑148 (MQ=255)
                               gCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTGTACCGt                                                                                                                >  2:2113852/1‑148 (MQ=255)
                               gCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGt                                                                                                                <  2:2626174/148‑1 (MQ=255)
                                    aGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTTATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCa                                                                                                           <  2:304011/148‑1 (MQ=255)
                                    aGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCa                                                                                                           <  1:2823191/148‑1 (MQ=255)
                                    aGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCa                                                                                                           <  2:2071641/148‑1 (MQ=255)
                                    aGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCa                                                                                                           <  1:412087/148‑1 (MQ=255)
                                     gCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAg                                                                                                          <  1:1665068/148‑1 (MQ=255)
                                     gCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAg                                                                                                          <  1:3403772/148‑1 (MQ=255)
                                      cTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAgg                                                                                                         >  2:3467991/1‑148 (MQ=255)
                                      cTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAgg                                                                                                         >  2:3467962/1‑148 (MQ=255)
                                         aaCGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCg                                                                                                      <  2:1393027/148‑1 (MQ=255)
                                         aaCGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCg                                                                                                      <  1:2321722/148‑1 (MQ=255)
                                          aCGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGt                                                                                                     <  2:1078853/148‑1 (MQ=255)
                                           cGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGtt                                                                                                    <  2:2412606/148‑1 (MQ=255)
                                            gtgtTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTa                                                                                                   <  1:2117206/148‑1 (MQ=255)
                                               ttCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGa                                                                                                <  2:278305/148‑1 (MQ=255)
                                               ttCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGa                                                                                                <  2:278865/148‑1 (MQ=255)
                                                tCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGAt                                                                                               <  2:1504608/148‑1 (MQ=255)
                                                  gAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTa                                                                                             <  2:1462175/148‑1 (MQ=255)
                                                  gAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTa                                                                                             <  2:173110/148‑1 (MQ=255)
                                                  gAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTa                                                                                             <  2:172657/148‑1 (MQ=255)
                                                   aaGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTaa                                                                                            <  1:3139809/148‑1 (MQ=255)
                                                      gTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGa                                                                                         >  1:1935228/1‑148 (MQ=255)
                                                         aaCGTGTTGAACGTGGTGACGTAATTTCCGACGTTCCGGAAGCGCCGCACTACTTTCTTCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATTCAGTCTGCAGTGCGTTAAGATTATCGATat                                                                                      <  2:485240/148‑2 (MQ=255)
                                                         aaCGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATaa                                                                                      <  1:270922/148‑1 (MQ=255)
                                                          aCGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATaaa                                                                                     <  2:3215545/148‑1 (MQ=255)
                                                          aCGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATaaa                                                                                     <  1:2684736/148‑1 (MQ=255)
                                                           cGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCTTCTGCAGGGCGTTAAGATTAACGAGAAac                                                                                    >  1:2382026/1‑148 (MQ=255)
                                                           cGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAac                                                                                    >  1:1994162/1‑148 (MQ=255)
                                                           cGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAac                                                                                    >  1:1169883/1‑148 (MQ=255)
                                                               tAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACAt                                                                                >  1:2574528/1‑148 (MQ=255)
                                                                  aaCGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGa                                                                             <  1:3472502/148‑1 (MQ=255)
                                                                  aaCGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGa                                                                             <  1:799658/148‑1 (MQ=255)
                                                                     gtggtgACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGt                                                                          <  2:1798791/148‑1 (MQ=255)
                                                                        gtgACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTAt                                                                       <  1:2778626/148‑1 (MQ=255)
                                                                         tgACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTAtc                                                                      <  1:3016474/148‑1 (MQ=255)
                                                                           aCGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTAtcgt                                                                    >  2:2541821/1‑148 (MQ=255)
                                                                                aTTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTc                                                               <  1:662069/148‑1 (MQ=255)
                                                                                   tCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGa                                                            >  2:2704998/1‑148 (MQ=255)
                                                                                     cGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGAtg                                                          <  2:3426327/148‑1 (MQ=255)
                                                                                        cGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGAtgctg                                                       <  1:7131/148‑1 (MQ=255)
                                                                                          gTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCg                                                     <  1:3288185/148‑1 (MQ=255)
                                                                                             cGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTaa                                                  <  2:1571465/148‑1 (MQ=255)
                                                                                               gAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAg                                                <  2:3082624/148‑1 (MQ=255)
                                                                                                aaGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGc                                               <  1:1131248/148‑1 (MQ=255)
                                                                                                aaGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGc                                               >  1:55138/1‑148 (MQ=255)
                                                                                                   cgccgcACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTAc                                            >  1:943494/1‑148 (MQ=255)
                                                                                                        cACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCTTTCGTCAGATGCTGCGTAAAGCTACCATCg                                       >  2:2059890/1‑148 (MQ=255)
                                                                                                          cGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGtt                                     <  1:1030615/148‑1 (MQ=255)
                                                                                                           gACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTa                                    <  2:3228091/148‑1 (MQ=255)
                                                                                                           gACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTa                                    <  1:3202048/148‑1 (MQ=255)
                                                                                                           gACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTa                                    <  2:1233143/148‑1 (MQ=255)
                                                                                                            aCATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTaa                                   <  1:2947999/148‑1 (MQ=255)
                                                                                                            aCATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCAGCGTTaa                                   <  1:2220217/148‑1 (MQ=255)
                                                                                                             cATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAc                                  >  2:408537/1‑148 (MQ=255)
                                                                                                              aTTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAcg                                 >  2:2942941/1‑148 (MQ=255)
                                                                                                                 ctgcgtctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCgg                              >  2:1346922/1‑148 (MQ=255)
                                                                                                                 ctgcgtctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCgg                              >  2:1347097/1‑148 (MQ=255)
                                                                                                                  tgcgtctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCggg                             >  1:178797/1‑148 (MQ=255)
                                                                                                                   gcgtctgcgtGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGt                            <  1:2273709/148‑1 (MQ=255)
                                                                                                                              gtgtTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACtt                 <  2:2341598/148‑1 (MQ=255)
                                                                                                                               tgtTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTc                <  2:2048074/148‑1 (MQ=255)
                                                                                                                               tgtTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTc                <  2:1642564/148‑1 (MQ=255)
                                                                                                                               tgtTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTc                <  1:2278796/148‑1 (MQ=255)
                                                                                                                                  tCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTg             >  1:2866163/1‑148 (MQ=255)
                                                                                                                                      gCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAg         >  2:315301/1‑148 (MQ=255)
                                                                                                                                      gCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAg         <  1:1701552/148‑1 (MQ=255)
                                                                                                                                       cTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAgg        >  1:1947363/1‑148 (MQ=255)
                                                                                                                                       cTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAgg        <  2:809064/148‑1 (MQ=255)
                                                                                                                                       cTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACACCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAgg        <  2:551688/148‑1 (MQ=255)
                                                                                                                                         gTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCg      <  2:733373/148‑1 (MQ=255)
                                                                                                                                          ttACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGa     >  1:2065102/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACA  >  CP009273/4178825‑4179113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTG  >  CP009273/4178821‑4179118
                                                                                                                                                    |                                                                                                                                                     
GGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTT                                                                                                                                                      <  SRR19973065.1577475/150‑1 (MQ=60)
     CGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGACCGTGGTGNCGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACAT                                                                                                                                                 <  SRR19973065.1797372/150‑1 (MQ=60)
     CGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGACCGTGGTGNCGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACAT                                                                                                                                                 <  SRR19973065.1797927/150‑1 (MQ=60)
       ATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCG                                                                                                                                               <  SRR19973065.3067610/150‑1 (MQ=60)
         CCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTT                                                                                                                                             >  SRR19973065.593921/1‑150 (MQ=60)
         CCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTT                                                                                                                                             >  SRR19973065.593679/1‑150 (MQ=60)
         CCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTT                                                                                                                                             <  SRR19973065.800272/150‑1 (MQ=60)
               GAAGAGATGATTCCGAATTGGCGTCAGCTCATCGTGTTAGAAGGTGAACGTGTTGATCGTGGTGACGTAATTTCGGACGGTCCGGAAGCTCCGAACGAAATTCTTCGTCTGCGTGGTGTTCATGCTGTTACTATTTACATCGTTAACGAA                                                                                                                                       <  SRR19973065.365173/150‑1 (MQ=60)
                AAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAG                                                                                                                                      <  SRR19973065.1466113/150‑1 (MQ=60)
                AAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAG                                                                                                                                      <  SRR19973065.1466120/150‑1 (MQ=60)
                AAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAG                                                                                                                                      >  SRR19973065.1212645/1‑150 (MQ=60)
                   AGATGTTTCCGAAAGGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTAC                                                                                                                                   <  SRR19973065.2674355/150‑1 (MQ=60)
                     ATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAG                                                                                                                                 <  SRR19973065.141016/150‑1 (MQ=60)
                     ATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAG                                                                                                                                 <  SRR19973065.600293/150‑1 (MQ=60)
                      TGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGG                                                                                                                                >  SRR19973065.1319021/1‑150 (MQ=60)
                      TGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGG                                                                                                                                <  SRR19973065.832382/150‑1 (MQ=60)
                           CCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTA                                                                                                                           <  SRR19973065.3163163/150‑1 (MQ=60)
                           CCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTA                                                                                                                           <  SRR19973065.3163780/150‑1 (MQ=60)
                             GAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATA                                                                                                                         <  SRR19973065.1574432/150‑1 (MQ=60)
                              AAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATAC                                                                                                                        >  SRR19973065.1516179/1‑150 (MQ=60)
                               AATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACC                                                                                                                       <  SRR19973065.1477516/150‑1 (MQ=60)
                                        AGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGG                                                                                                              <  SRR19973065.2840627/150‑1 (MQ=60)
                                        AGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGG                                                                                                              <  SRR19973065.414969/150‑1 (MQ=60)
                                        AGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGG                                                                                                              <  SRR19973065.2709386/150‑1 (MQ=60)
                                         GCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGG                                                                                                             <  SRR19973065.1676217/150‑1 (MQ=60)
                                         GCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGG                                                                                                             <  SRR19973065.3424572/150‑1 (MQ=60)
                                          CTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGC                                                                                                            >  SRR19973065.2491530/1‑150 (MQ=60)
                                           TCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCG                                                                                                           <  SRR19973065.2884325/150‑1 (MQ=60)
                                           TCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCG                                                                                                           >  SRR19973065.1081251/1‑150 (MQ=60)
                                             AACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTT                                                                                                         <  SRR19973065.2336271/150‑1 (MQ=60)
                                                GTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAG                                                                                                      <  SRR19973065.2130832/150‑1 (MQ=60)
                                                   TTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATT                                                                                                   <  SRR19973065.3089920/150‑1 (MQ=60)
                                                   TTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATT                                                                                                   <  SRR19973065.2303763/150‑1 (MQ=60)
                                                      GAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAAC                                                                                                <  SRR19973065.71846/150‑1 (MQ=60)
                                                       AAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACG                                                                                               <  SRR19973065.3158897/150‑1 (MQ=60)
                                                        AGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGA                                                                                              >  SRR19973065.1947895/1‑150 (MQ=60)
                                                          GTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATA                                                                                            <  SRR19973065.1858305/150‑1 (MQ=60)
                                                             AACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCTTCTGCAGGGCGTTAAGATTAACGAGAAAC                                                                                         >  SRR19973065.2396944/1‑150 (MQ=60)
                                                             AACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAAC                                                                                         >  SRR19973065.538958/1‑150 (MQ=60)
                                                             AACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAAC                                                                                         <  SRR19973065.848858/150‑1 (MQ=60)
                                                             AACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAAC                                                                                         <  SRR19973065.272753/150‑1 (MQ=60)
                                                             AACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAAC                                                                                         >  SRR19973065.2007087/1‑150 (MQ=60)
                                                             AACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAAC                                                                                         >  SRR19973065.1177340/1‑150 (MQ=60)
                                                             AACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAAC                                                                                         >  SRR19973065.2396926/1‑150 (MQ=60)
                                                              ACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACA                                                                                        <  SRR19973065.2701388/150‑1 (MQ=60)
                                                               CGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACAC                                                                                       <  SRR19973065.2013951/150‑1 (MQ=60)
                                                                 TGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACAT                                                                                     >  SRR19973065.2590590/1‑150 (MQ=60)
                                                                  GTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATC                                                                                    <  SRR19973065.1706530/150‑1 (MQ=60)
                                                                      AACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAG                                                                                <  SRR19973065.3493636/150‑1 (MQ=60)
                                                                      AACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAG                                                                                <  SRR19973065.3493646/150‑1 (MQ=60)
                                                                      AACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAG                                                                                <  SRR19973065.804942/150‑1 (MQ=60)
                                                                           GGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATC                                                                           <  SRR19973065.20940/150‑1 (MQ=60)
                                                                            GTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCG                                                                          <  SRR19973065.2793558/150‑1 (MQ=60)
                                                                            GTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCG                                                                          <  SRR19973065.2795870/150‑1 (MQ=60)
                                                                             TGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGT                                                                         <  SRR19973065.3034953/150‑1 (MQ=60)
                                                                                CGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCG                                                                      >  SRR19973065.2811761/1‑150 (MQ=60)
                                                                                  TAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTC                                                                    >  SRR19973065.3359518/1‑150 (MQ=60)
                                                                                    ATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAG                                                                  <  SRR19973065.666453/150‑1 (MQ=60)
                                                                                         CGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCT                                                             <  SRR19973065.3451655/150‑1 (MQ=60)
                                                                                         CGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCT                                                             >  SRR19973065.1725199/1‑150 (MQ=60)
                                                                                            CGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCG                                                          <  SRR19973065.7244/150‑1 (MQ=60)
                                                                                              GTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTA                                                        <  SRR19973065.3308193/150‑1 (MQ=60)
                                                                                                  GGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGC                                                    >  SRR19973065.55735/1‑150 (MQ=60)
                                                                                                    AAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTA                                                  <  SRR19973065.1138757/150‑1 (MQ=60)
                                                                                                    AAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTA                                                  <  SRR19973065.1138595/150‑1 (MQ=60)
                                                                                                     AGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTAC                                                 >  SRR19973065.949729/1‑150 (MQ=60)
                                                                                                             ACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTA                                         <  SRR19973065.3050403/150‑1 (MQ=60)
                                                                                                              CGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAA                                        <  SRR19973065.1037442/150‑1 (MQ=60)
                                                                                                               GACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAC                                       <  SRR19973065.1405230/150‑1 (MQ=60)
                                                                                                               GACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAC                                       <  SRR19973065.3221326/150‑1 (MQ=60)
                                                                                                               GACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAC                                       <  SRR19973065.2262563/150‑1 (MQ=60)
                                                                                                               GACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAAC                                       >  SRR19973065.404192/1‑150 (MQ=60)
                                                                                                                ACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACG                                      <  SRR19973065.2968973/150‑1 (MQ=60)
                                                                                                                ACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACG                                      <  SRR19973065.2965939/150‑1 (MQ=60)
                                                                                                                ACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCAGCGTTAACG                                      <  SRR19973065.2234386/150‑1 (MQ=60)
                                                                                                                  ATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCG                                    >  SRR19973065.3211433/1‑150 (MQ=60)
                                                                                                                    TCTGCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGG                                  >  SRR19973065.180209/1‑150 (MQ=60)
                                                                                                                       GCGTGTGCGTGGTGTTGATGCTGTTACTCTTTACATCGTTAACGAAGTAGAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAG                               <  SRR19973065.2288067/150‑1 (MQ=60)
                                                                                                                       GCGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAG                               <  SRR19973065.2288061/150‑1 (MQ=60)
                                                                                                                        CGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGC                              >  SRR19973065.814407/1‑150 (MQ=60)
                                                                                                                        CGTCTGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACAACGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGC                              >  SRR19973065.555231/1‑150 (MQ=60)
                                                                                                                            TGCGTGGTGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCG                          <  SRR19973065.3234648/150‑1 (MQ=60)
                                                                                                                              CGTGGTGTTCATTCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGAC                        <  SRR19973065.218650/150‑1 (MQ=60)
                                                                                                                              CGTGGTGGTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGAC                        <  SRR19973065.2004147/150‑1 (MQ=60)
                                                                                                                                   TGTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCT                   <  SRR19973065.2293169/150‑1 (MQ=60)
                                                                                                                                    GTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTG                  >  SRR19973065.2883751/1‑150 (MQ=60)
                                                                                                                                    GTTCATGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTG                  >  SRR19973065.2883058/1‑150 (MQ=60)
                                                                                                                                         TGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGG             >  SRR19973065.1957942/1‑150 (MQ=60)
                                                                                                                                         TGCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGG             >  SRR19973065.1960090/1‑150 (MQ=60)
                                                                                                                                          GCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGC            <  SRR19973065.1712892/150‑1 (MQ=60)
                                                                                                                                          GCTGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGC            <  SRR19973065.665377/150‑1 (MQ=60)
                                                                                                                                            TGTTACTCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGA          >  SRR19973065.2078263/1‑150 (MQ=60)
                                                                                                                                                  TCTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGT    <  SRR19973065.3093604/150‑1 (MQ=60)
                                                                                                                                                   CTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTT   <  SRR19973065.1951620/150‑1 (MQ=60)
                                                                                                                                                   CTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTT   <  SRR19973065.753907/150‑1 (MQ=60)
                                                                                                                                                   CTTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTT   <  SRR19973065.292395/150‑1 (MQ=60)
                                                                                                                                                    TTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTG  <  SRR19973065.437483/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GGTAGCGATCCGTACGAAGAGATGATTCCGAAATGGCGTCAGCTCAACGTGTTCGAAGGTGAACGTGTAGAACGTGGTGACGTAATTTCCGACGGTCCGGAAGCGCCGCACGACATTCTGCGTCTGCGTGGTGTTCATGCTGTTACTCGTTACATCGTTAACGAAGTACAGGACGTATACCGTCTGCAGGGCGTTAAGATTAACGATAAACACATCGAAGTTATCGTTCGTCAGATGCTGCGTAAAGCTACCATCGTTAACGCGGGTAGCTCCGACTTCCTGGAAGGCGAACAGGTTG  >  CP009273/4178821‑4179118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑