Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 302,428 G→T 100% P34Q (CCG→CAG)  ecpD ← polymerized tip adhesin of ECP fibers

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273302,4280GT95.0% 674.9 / 22.1 231P34Q (CCG→CAG) ecpDpolymerized tip adhesin of ECP fibers
Reads supporting (aligned to +/- strand):  ref base G (5/7);  new base T (97/122);  total (102/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 231 total aligned reads displayed.

GCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCC  >  CP009273/302283‑302571
                                                                                                                                                 |                                                                                                                                               
gCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTCACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGt                                                                                                                                               >  1:2273779/1‑148 (MQ=255)
   ttGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACTAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACACGCGTGCCTGTGCa                                                                                                                                            >  1:2968718/1‑148 (MQ=255)
   ttGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCa                                                                                                                                            >  1:3527343/1‑148 (MQ=255)
    tGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCAt                                                                                                                                           >  2:1956107/1‑148 (MQ=255)
    tGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCAt                                                                                                                                           <  1:762342/148‑1 (MQ=255)
      cTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATcc                                                                                                                                         >  2:132/1‑148 (MQ=255)
         aTAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGcc                                                                                                                                      <  2:639918/148‑1 (MQ=255)
         aTAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGcc                                                                                                                                      <  1:223165/148‑1 (MQ=255)
         aTAGATGGGTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGcc                                                                                                                                      <  1:1919350/148‑1 (MQ=255)
           aGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCa                                                                                                                                    >  1:1134493/1‑148 (MQ=255)
           aGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCa                                                                                                                                    >  2:2821051/1‑148 (MQ=255)
           aGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCa                                                                                                                                    >  1:3437085/1‑148 (MQ=255)
             aTGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAgg                                                                                                                                  >  1:3376711/1‑148 (MQ=255)
             aTGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAgg                                                                                                                                  <  2:186717/148‑1 (MQ=255)
                 ttCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGgttgt                                                                                                                              <  1:625550/148‑1 (MQ=255)
                 ttCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGgttgt                                                                                                                              >  2:1714289/1‑148 (MQ=255)
                   ccTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGttt                                                                                                                            <  2:2462709/148‑1 (MQ=255)
                   ccTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGttt                                                                                                                            <  2:1622494/148‑1 (MQ=255)
                     tGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTg                                                                                                                          >  1:2201369/1‑148 (MQ=255)
                       aaCCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCt                                                                                                                        >  2:1997841/1‑148 (MQ=255)
                             tgtATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGc                                                                                                                  >  2:391506/1‑148 (MQ=255)
                                aTTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGCGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGc                                                                                                               >  2:1489192/1‑148 (MQ=255)
                                 ttttAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGct                                                                                                              <  2:955331/148‑1 (MQ=255)
                                 ttttAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGct                                                                                                              <  2:2062794/148‑1 (MQ=255)
                                  tttAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGctc                                                                                                             <  1:1845548/148‑1 (MQ=255)
                                  tttAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGctc                                                                                                             <  2:3431014/148‑1 (MQ=255)
                                  tttAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGctc                                                                                                             <  1:1269427/148‑1 (MQ=255)
                                   ttAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGctct                                                                                                            >  1:1002197/1‑148 (MQ=255)
                                    tAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTg                                                                                                           >  2:2630729/1‑148 (MQ=255)
                                      aaCCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAg                                                                                                         >  2:3171515/1‑148 (MQ=255)
                                       aCCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAgc                                                                                                        >  2:2047010/1‑148 (MQ=255)
                                        ccGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAgcg                                                                                                       <  2:229166/148‑1 (MQ=255)
                                        ccGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAgcg                                                                                                       <  2:1476202/148‑1 (MQ=255)
                                           gTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAg                                                                                                    <  1:3103435/148‑1 (MQ=255)
                                           gTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAg                                                                                                    <  2:708352/148‑1 (MQ=255)
                                            tCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGt                                                                                                   <  2:2678516/148‑1 (MQ=255)
                                               aGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTAc                                                                                                <  1:214900/148‑1 (MQ=255)
                                                 cGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTg                                                                                              <  1:2813367/148‑1 (MQ=255)
                                                    ttGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGcc                                                                                           <  2:166979/148‑1 (MQ=255)
                                                    ttGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGcc                                                                                           >  1:3416952/1‑148 (MQ=255)
                                                       gCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGa                                                                                        <  2:1913387/148‑1 (MQ=255)
                                                          ccGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTa                                                                                     <  1:2639777/148‑1 (MQ=255)
                                                               cAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGcgcg                                                                                <  2:1090428/148‑1 (MQ=255)
                                                                aGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGa                                                                               <  2:3531661/148‑1 (MQ=255)
                                                                  gcgcGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGaaa                                                                             <  2:3527336/148‑1 (MQ=255)
                                                                  gcgcGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGaaa                                                                             <  2:3395110/148‑1 (MQ=255)
                                                                   cgcgGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGaaaa                                                                            <  2:1102505/148‑1 (MQ=255)
                                                                   cgcgGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGaaaa                                                                            <  2:1103128/148‑1 (MQ=255)
                                                                      ggATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTa                                                                         <  1:901579/148‑1 (MQ=255)
                                                                      ggATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTa                                                                         >  2:1838918/1‑148 (MQ=255)
                                                                        aTCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTCCTGGCCAGATTAGCGCGAAAATTatc                                                                       >  2:965866/1‑148 (MQ=255)
                                                                          ccAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTatcat                                                                     <  2:2129216/148‑1 (MQ=255)
                                                                          ccAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTGTTCGACAAACACAATCTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTatcat                                                                     <  2:1806097/148‑1 (MQ=255)
                                                                            aGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCg                                                                   <  2:1734146/148‑1 (MQ=255)
                                                                            aGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCg                                                                   >  1:2848536/1‑148 (MQ=255)
                                                                             gcgcCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGt                                                                  >  1:382007/1‑148 (MQ=255)
                                                                               gcCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGtta                                                                <  2:308326/148‑1 (MQ=255)
                                                                               gcCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGtta                                                                <  1:451984/148‑1 (MQ=255)
                                                                                ccccGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGttat                                                               <  2:3376711/148‑1 (MQ=255)
                                                                                    gCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATtagt                                                           <  1:3198991/148‑1 (MQ=255)
                                                                                    gCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATtagt                                                           <  2:347482/148‑1 (MQ=255)
                                                                                        ggAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTATCGCGAAAATTATAATCGTTATTAGTATGt                                                       >  1:603602/1‑148 (MQ=255)
                                                                                        ggAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGt                                                       >  1:602273/1‑148 (MQ=255)
                                                                                        ggAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGt                                                       <  2:1065108/148‑1 (MQ=255)
                                                                                           gTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTaa                                                    <  2:1131350/148‑1 (MQ=255)
                                                                                                gAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACtctc                                               <  1:3093994/148‑1 (MQ=255)
                                                                                                gAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACtctc                                               <  1:2759738/148‑1 (MQ=255)
                                                                                                 aaaaaGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGCTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCa                                              <  2:2697737/148‑1 (MQ=255)
                                                                                                  aaaaGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAt                                             <  2:291326/148‑1 (MQ=255)
                                                                                                  aaaaGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAt                                             <  2:3290060/148‑1 (MQ=255)
                                                                                                   aaaGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCTCACAGGTTGTTTTGCTCTCTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAtt                                            <  2:1579664/148‑1 (MQ=255)
                                                                                                   aaaGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAtt                                            <  1:3171613/148‑1 (MQ=255)
                                                                                                     aGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAttct                                          <  2:2922673/148‑1 (MQ=255)
                                                                                                       ttGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAttctct                                        >  1:1172364/1‑148 (MQ=255)
                                                                                                          tcgtcTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAttctcttct                                     >  1:1625094/1‑148 (MQ=255)
                                                                                                           cgtcTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAttctcttctc                                    >  1:2765367/1‑148 (MQ=255)
                                                                                                            gtcTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTcc                                   >  1:1795120/1‑148 (MQ=255)
                                                                                                              cTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTg                                 >  2:3309194/1‑148 (MQ=255)
                                                                                                               tGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGt                                >  2:1857474/1‑148 (MQ=255)
                                                                                                                gAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTc                               >  1:3431839/1‑148 (MQ=255)
                                                                                                                 aGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCt                              >  2:1165754/1‑148 (MQ=255)
                                                                                                                 aGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGGTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCt                              >  2:1439566/1‑148 (MQ=255)
                                                                                                                     gttTTAGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGt                          >  2:75062/1‑148 (MQ=255)
                                                                                                                       tttCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCa                        >  2:2811137/1‑148 (MQ=255)
                                                                                                                               aacacaaacTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCgccgcc                >  1:155851/1‑148 (MQ=255)
                                                                                                                                acacaaacTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGccc               >  1:2509453/1‑148 (MQ=255)
                                                                                                                                acacaaacTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGccc               >  2:2857099/1‑148 (MQ=255)
                                                                                                                                acacaaacTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGccc               <  2:3005897/148‑1 (MQ=255)
                                                                                                                                 cacaaacTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCa              >  1:2094984/1‑148 (MQ=255)
                                                                                                                                   caaacTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACg            >  2:2243666/1‑148 (MQ=255)
                                                                                                                                   caaacTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACg            >  1:2575382/1‑148 (MQ=255)
                                                                                                                                   caaacTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACg            >  1:1770904/1‑148 (MQ=255)
                                                                                                                                       cTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATga        >  2:9066/1‑148 (MQ=255)
                                                                                                                                       cTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATga        >  2:968273/1‑148 (MQ=255)
                                                                                                                                       cTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATga        <  2:578077/148‑1 (MQ=255)
                                                                                                                                        tCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATgag       <  1:1689056/148‑1 (MQ=255)
                                                                                                                                          gcgTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGagag     >  2:2431985/1‑148 (MQ=255)
                                                                                                                                          gcgTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGagag     >  2:1134560/1‑148 (MQ=255)
                                                                                                                                             tGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGcc  >  2:1461574/1‑148 (MQ=255)
                                                                                                                                             tGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGcc  >  1:1865034/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCC  >  CP009273/302283‑302571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGC  >  CP009273/302279‑302576
                                                                                                                                                     |                                                                                                                                                    
CGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCT                                                                                                                                                      >  SRR19973067.1110003/1‑150 (MQ=60)
CGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCT                                                                                                                                                      >  SRR19973067.1110004/1‑150 (MQ=60)
CGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCT                                                                                                                                                      >  SRR19973067.1110629/1‑150 (MQ=60)
CGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTAGCGTGCCT                                                                                                                                                      >  SRR19973067.2895656/1‑150 (MQ=60)
  AGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTCACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGT                                                                                                                                                    >  SRR19973067.2288830/1‑150 (MQ=60)
   GGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTG                                                                                                                                                   <  SRR19973067.132536/150‑1 (MQ=60)
     CTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACTAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACACGCGTGCCTGTGCA                                                                                                                                                 >  SRR19973067.2987454/1‑150 (MQ=60)
     CTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCA                                                                                                                                                 >  SRR19973067.2986598/1‑150 (MQ=60)
     CTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCA                                                                                                                                                 >  SRR19973067.3549642/1‑150 (MQ=60)
     CTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCA                                                                                                                                                 >  SRR19973067.3549649/1‑150 (MQ=60)
        TGCTGATAGCTGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCC                                                                                                                                              <  SRR19973067.22018/150‑1 (MQ=60)
        TGCTGATAGATGGTTCCTGAACCTGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCC                                                                                                                                              <  SRR19973067.767637/150‑1 (MQ=60)
             ATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCA                                                                                                                                         >  SRR19973067.3458969/1‑150 (MQ=60)
             ATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCA                                                                                                                                         >  SRR19973067.3458946/1‑150 (MQ=60)
             ATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCA                                                                                                                                         >  SRR19973067.1142190/1‑150 (MQ=60)
             ATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCA                                                                                                                                         >  SRR19973067.1142135/1‑150 (MQ=60)
             ATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCA                                                                                                                                         <  SRR19973067.225061/150‑1 (MQ=60)
             ATAGATGGGTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCA                                                                                                                                         <  SRR19973067.224790/150‑1 (MQ=60)
             ATAGATGGGTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCA                                                                                                                                         <  SRR19973067.1932445/150‑1 (MQ=60)
               AGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGG                                                                                                                                       >  SRR19973067.3398175/1‑150 (MQ=60)
               AGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGG                                                                                                                                       >  SRR19973067.1625107/1‑150 (MQ=60)
                 ATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTT                                                                                                                                     >  SRR19973067.2044308/1‑150 (MQ=60)
                     TTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTT                                                                                                                                 <  SRR19973067.629690/150‑1 (MQ=60)
                      TCCTGAACGAGTTTATTTTAAACCGGTCCAGCGGTGGGCACCTGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTT                                                                                                                                <  SRR19973067.377026/150‑1 (MQ=60)
                       CCTGAACCAGTGTATTTTACACCGGGCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTNACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTG                                                                                                                               <  SRR19973067.1816482/150‑1 (MQ=60)
                       CCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTG                                                                                                                               >  SRR19973067.2216052/1‑150 (MQ=60)
                       CCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTG                                                                                                                               >  SRR19973067.507555/1‑150 (MQ=60)
                       CCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTG                                                                                                                               <  SRR19973067.2804382/150‑1 (MQ=60)
                            ACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCAC                                                                                                                          >  SRR19973067.1864185/1‑150 (MQ=60)
                             CCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACT                                                                                                                         <  SRR19973067.3452308/150‑1 (MQ=60)
                                    ATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTC                                                                                                                  >  SRR19973067.3118862/1‑150 (MQ=60)
                                     TTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCT                                                                                                                 >  SRR19973067.1009085/1‑150 (MQ=60)
                                      TTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTG                                                                                                                <  SRR19973067.1862082/150‑1 (MQ=60)
                                      TTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTG                                                                                                                <  SRR19973067.1858128/150‑1 (MQ=60)
                                      TTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTG                                                                                                                <  SRR19973067.1278060/150‑1 (MQ=60)
                                        TAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAG                                                                                                              <  SRR19973067.803023/150‑1 (MQ=60)
                                               GTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTT                                                                                                       <  SRR19973067.3123178/150‑1 (MQ=60)
                                                 CCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTAC                                                                                                     >  SRR19973067.2241441/1‑150 (MQ=60)
                                                  CAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACT                                                                                                    <  SRR19973067.1105712/150‑1 (MQ=60)
                                                   AGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTG                                                                                                   <  SRR19973067.216490/150‑1 (MQ=60)
                                                   AGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTG                                                                                                   <  SRR19973067.216485/150‑1 (MQ=60)
                                                   AGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTG                                                                                                   >  SRR19973067.1900161/1‑150 (MQ=60)
                                                     CGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACACACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTGTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGC                                                                                                 >  SRR19973067.1804217/1‑150 (MQ=60)
                                                     CGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGC                                                                                                 <  SRR19973067.2831718/150‑1 (MQ=60)
                                                     CGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGC                                                                                                 <  SRR19973067.2831492/150‑1 (MQ=60)
                                                      GGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCC                                                                                                >  SRR19973067.3438696/1‑150 (MQ=60)
                                                      GGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCC                                                                                                >  SRR19973067.3438701/1‑150 (MQ=60)
                                                              CCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGC                                                                                        <  SRR19973067.2656838/150‑1 (MQ=60)
                                                                          GGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATC                                                                            <  SRR19973067.907519/150‑1 (MQ=60)
                                                                          GGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATC                                                                            <  SRR19973067.1189713/150‑1 (MQ=60)
                                                                              CCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCG                                                                        >  SRR19973067.2866810/1‑150 (MQ=60)
                                                                              CCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCG                                                                        >  SRR19973067.2866802/1‑150 (MQ=60)
                                                                               CAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGT                                                                       >  SRR19973067.384669/1‑150 (MQ=60)
                                                                                 GCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTA                                                                     <  SRR19973067.240266/150‑1 (MQ=60)
                                                                                 GCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTA                                                                     <  SRR19973067.649331/150‑1 (MQ=60)
                                                                                   GCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATT                                                                   <  SRR19973067.1726043/150‑1 (MQ=60)
                                                                                   GCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATT                                                                   <  SRR19973067.455122/150‑1 (MQ=60)
                                                                                   GCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATT                                                                   <  SRR19973067.1726044/150‑1 (MQ=60)
                                                                                        GCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAG                                                              <  SRR19973067.1281763/150‑1 (MQ=60)
                                                                                        GCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAG                                                              <  SRR19973067.3219263/150‑1 (MQ=60)
                                                                                        GCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAG                                                              <  SRR19973067.3219171/150‑1 (MQ=60)
                                                                                        GCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAG                                                              <  SRR19973067.3219154/150‑1 (MQ=60)
                                                                                         CCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGG                                                             <  SRR19973067.504812/150‑1 (MQ=60)
                                                                                         CCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATAAGTAGG                                                             <  SRR19973067.2142650/150‑1 (MQ=60)
                                                                                          CAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTATCGCGAAAATTATAATCGTTATTAGTATGT                                                            >  SRR19973067.607436/1‑150 (MQ=60)
                                                                                          CAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGT                                                            >  SRR19973067.606083/1‑150 (MQ=60)
                                                                                          CAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCAAAGTTATTAGTAGGT                                                            >  SRR19973067.607455/1‑150 (MQ=60)
                                                                                                TGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTC                                                      >  SRR19973067.3419985/1‑150 (MQ=60)
                                                                                                TGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTC                                                      >  SRR19973067.1645547/1‑150 (MQ=60)
                                                                                                   CGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCA                                                   >  SRR19973067.433226/1‑150 (MQ=60)
                                                                                                    GAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAT                                                  <  SRR19973067.3113671/150‑1 (MQ=60)
                                                                                                    GAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAT                                                  <  SRR19973067.2777572/150‑1 (MQ=60)
                                                                                                       AAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCT                                               <  SRR19973067.3191695/150‑1 (MQ=60)
                                                                                                       AAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCT                                               <  SRR19973067.3191595/150‑1 (MQ=60)
                                                                                                         AGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCT                                             >  SRR19973067.1180135/1‑150 (MQ=60)
                                                                                                            TGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCT                                          <  SRR19973067.2444585/150‑1 (MQ=60)
                                                                                                            TGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCT                                          >  SRR19973067.1636243/1‑150 (MQ=60)
                                                                                                             GTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTC                                         >  SRR19973067.3535019/1‑150 (MQ=60)
                                                                                                             GTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTC                                         >  SRR19973067.2783236/1‑150 (MQ=60)
                                                                                                             GTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTATCATTCTCTTCTC                                         >  SRR19973067.2782476/1‑150 (MQ=60)
                                                                                                              TCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCC                                        >  SRR19973067.1807199/1‑150 (MQ=60)
                                                                                                                  CTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTC                                    >  SRR19973067.3453680/1‑150 (MQ=60)
                                                                                                                         GTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCA                             <  SRR19973067.2727403/150‑1 (MQ=60)
                                                                                                                         GTGTTCGACAAACACAAACTCGCGTGGCTGTGCATCCGCCCATGTTGTTTTGCTCACTGCCGCTCTGAGAGCATTTTCTTGCAAGATTAGCGCGAAAATTATCATCGTTATTAGTATGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCA                             <  SRR19973067.635964/150‑1 (MQ=60)
                                                                                                                              CGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCC                        >  SRR19973067.2739827/1‑150 (MQ=60)
                                                                                                                                 CAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCC                     >  SRR19973067.157160/1‑150 (MQ=60)
                                                                                                                                  AAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCC                    >  SRR19973067.2608029/1‑150 (MQ=60)
                                                                                                                                  AAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCC                    >  SRR19973067.2525967/1‑150 (MQ=60)
                                                                                                                                  AAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCC                    >  SRR19973067.2525917/1‑150 (MQ=60)
                                                                                                                                   AACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCA                   >  SRR19973067.2108846/1‑150 (MQ=60)
                                                                                                                                   AACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGGTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCA                   >  SRR19973067.606605/1‑150 (MQ=60)
                                                                                                                                    ACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCAC                  >  SRR19973067.1341581/1‑150 (MQ=60)
                                                                                                                                     CACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACG                 >  SRR19973067.3069567/1‑150 (MQ=60)
                                                                                                                                     CACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACG                 >  SRR19973067.2592176/1‑150 (MQ=60)
                                                                                                                                     CACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACG                 >  SRR19973067.3118743/1‑150 (MQ=60)
                                                                                                                                     CACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACG                 >  SRR19973067.1782923/1‑150 (MQ=60)
                                                                                                                                            TCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAG          <  SRR19973067.1700687/150‑1 (MQ=60)
                                                                                                                                               CGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCC       >  SRR19973067.1877759/1‑150 (MQ=60)
                                                                                                                                                  GCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCT    >  SRR19973067.1992196/1‑150 (MQ=60)
                                                                                                                                                  GCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCT    >  SRR19973067.1992142/1‑150 (MQ=60)
                                                                                                                                                    CTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGC  >  SRR19973067.690067/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGC  >  CP009273/302279‑302576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑