Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 302,428 G→T 100% P34Q (CCG→CAG)  ecpD ← polymerized tip adhesin of ECP fibers

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273302,4280GT97.1% 795.0 / 10.7 254P34Q (CCG→CAG) ecpDpolymerized tip adhesin of ECP fibers
Reads supporting (aligned to +/- strand):  ref base G (5/3);  new base T (121/125);  total (126/128)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 257 total aligned reads displayed.

AGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCT  >  CP009273/302281‑302574
                                                                                                                                                   |                                                                                                                                                  
 ggCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCtgtg                                                                                                                                                 <  2:3936165/150‑1 (MQ=255)
 ggCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCtgtg                                                                                                                                                 >  2:3645836/1‑150 (MQ=255)
  gCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCCGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGc                                                                                                                                                <  2:4261534/150‑1 (MQ=255)
  gCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGc                                                                                                                                                >  2:3050681/1‑150 (MQ=255)
   cTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCa                                                                                                                                               >  1:2435625/1‑150 (MQ=255)
    tttGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCAt                                                                                                                                              >  2:3825641/1‑150 (MQ=255)
       gCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCg                                                                                                                                           <  1:680749/150‑1 (MQ=255)
           aTAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCa                                                                                                                                       <  2:3641529/150‑1 (MQ=255)
           aTAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCa                                                                                                                                       <  2:1941752/150‑1 (MQ=255)
           aTAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCa                                                                                                                                       <  2:1819720/150‑1 (MQ=255)
             aGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAgg                                                                                                                                     <  1:1872357/150‑1 (MQ=255)
               aTGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGgtt                                                                                                                                   <  2:3764710/150‑1 (MQ=255)
               aTGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGgtt                                                                                                                                   <  2:3593804/150‑1 (MQ=255)
               aTGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGgtt                                                                                                                                   >  2:1049645/1‑150 (MQ=255)
                   ttCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGttt                                                                                                                               >  2:539201/1‑150 (MQ=255)
                           ccAGTGTATTTTAAACCGGTCCCGCGGGTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACt                                                                                                                       <  2:1148777/150‑1 (MQ=255)
                           ccAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACt                                                                                                                       <  1:3905430/150‑1 (MQ=255)
                              gtgtATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGcc                                                                                                                    <  2:243413/150‑1 (MQ=255)
                               tgtATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCg                                                                                                                   >  2:851885/1‑150 (MQ=255)
                                 tATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGct                                                                                                                 <  2:593502/150‑1 (MQ=255)
                                 tATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGct                                                                                                                 >  1:2610364/1‑150 (MQ=255)
                                   ttttAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGctct                                                                                                               <  2:2143149/150‑1 (MQ=255)
                                   ttttAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGctca                                                                                                               <  2:3012549/150‑2 (MQ=255)
                                     ttAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGa                                                                                                             <  2:4909251/150‑1 (MQ=255)
                                       aaaCCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAgc                                                                                                           >  1:2906692/1‑150 (MQ=255)
                                       aaaCCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAgc                                                                                                           >  2:515999/1‑150 (MQ=255)
                                        aaCCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAgcg                                                                                                          <  1:3238111/150‑1 (MQ=255)
                                         aCCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAgcgc                                                                                                         >  1:2303525/1‑150 (MQ=255)
                                           cGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAg                                                                                                       >  2:1665528/1‑150 (MQ=255)
                                           cGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAg                                                                                                       <  1:155662/150‑1 (MQ=255)
                                            ggTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGt                                                                                                      >  2:2370438/1‑150 (MQ=255)
                                            ggTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGt                                                                                                      >  1:4633829/1‑150 (MQ=255)
                                            ggTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGt                                                                                                      >  2:2183385/1‑150 (MQ=255)
                                            ggTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGt                                                                                                      >  2:2183424/1‑150 (MQ=255)
                                             gTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTGTTGCTCACTGCCGCTCTGAGCGCAGtt                                                                                                     >  1:631342/1‑150 (MQ=255)
                                             gTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGtt                                                                                                     <  1:3818667/150‑1 (MQ=255)
                                             gTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGtt                                                                                                     <  1:4294901/150‑1 (MQ=255)
                                              tCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTa                                                                                                    >  1:2112489/1‑150 (MQ=255)
                                              tCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTa                                                                                                    <  1:2629844/150‑1 (MQ=255)
                                              tCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTa                                                                                                    >  1:1408733/1‑150 (MQ=255)
                                                 aGCGGTTGGCACCGGTCAGGCGCGGATCAAGCGCCCCGCCAGGAGTGACGAAAAATTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCATCTCTGAGCGCAGTTACTg                                                                                                 >  1:1269467/1‑150 (MQ=255)
                                                  gCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTgg                                                                                                <  1:4378554/150‑1 (MQ=255)
                                                   cGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGc                                                                                               <  2:2094026/150‑1 (MQ=255)
                                                     gTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCa                                                                                             >  1:4324982/1‑150 (MQ=255)
                                                        ggCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGAt                                                                                          >  1:573135/1‑150 (MQ=255)
                                                        ggCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGAt                                                                                          >  2:4589796/1‑150 (MQ=255)
                                                        ggCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGAt                                                                                          >  1:1164074/1‑150 (MQ=255)
                                                          cACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTa                                                                                        <  2:699261/150‑1 (MQ=255)
                                                           aCCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAg                                                                                       <  1:4616032/150‑1 (MQ=255)
                                                           aCCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAg                                                                                       <  2:2391068/150‑1 (MQ=255)
                                                            ccGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAgc                                                                                      <  2:4478944/150‑1 (MQ=255)
                                                               gTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGcgcg                                                                                   <  1:1108251/150‑1 (MQ=255)
                                                                  aGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTCCTGGCCAGATTAGCGCGaaa                                                                                >  1:2733969/1‑150 (MQ=255)
                                                                  aGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGaaa                                                                                >  1:1666792/1‑150 (MQ=255)
                                                                   ggCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGaaaa                                                                               <  2:5033649/150‑1 (MQ=255)
                                                                      gcgGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTa                                                                            >  2:1489091/1‑150 (MQ=255)
                                                                      gcgGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTa                                                                            <  1:4676416/150‑1 (MQ=255)
                                                                           tCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTatcatc                                                                       <  2:1020947/150‑1 (MQ=255)
                                                                           tCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTatcatc                                                                       <  1:1924228/150‑1 (MQ=255)
                                                                            ccAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCg                                                                      >  2:347989/1‑150 (MQ=255)
                                                                                  ccccGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGttatta                                                                >  1:3730852/1‑150 (MQ=255)
                                                                                   cccGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATtag                                                               <  2:2662210/150‑1 (MQ=255)
                                                                                     cGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATtagta                                                             <  2:3562863/150‑1 (MQ=255)
                                                                                       ccAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCCGTTACTGGCCAGATTAGCGCGACAATTATCATCGTTCTTAGTAgg                                                           >  1:893183/1‑150 (MQ=255)
                                                                                       ccAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAgg                                                           >  1:5065411/1‑150 (MQ=255)
                                                                                       ccAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAgg                                                           >  2:4750113/1‑150 (MQ=255)
                                                                                       ccAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAgg                                                           >  1:341213/1‑150 (MQ=255)
                                                                                              tGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAActc                                                    <  2:283420/150‑1 (MQ=255)
                                                                                                 cGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCa                                                 <  2:4103539/150‑1 (MQ=255)
                                                                                                 cGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCa                                                 >  1:1812821/1‑150 (MQ=255)
                                                                                                  gAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAt                                                <  2:3131720/150‑1 (MQ=255)
                                                                                                   aaaaaGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAtt                                               >  1:360836/1‑150 (MQ=255)
                                                                                                    aaaaGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAttc                                              <  2:794707/150‑1 (MQ=255)
                                                                                                    aaaaGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGGTATTAGTAGGTTAACTCTCAtta                                              >  1:3937630/1‑149 (MQ=255)
                                                                                                     aaaGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAttct                                             <  1:828666/150‑1 (MQ=255)
                                                                                                      aaGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTCTTAGTAGGGGAACTCGCAgtcgc                                            >  2:4362534/1‑148 (MQ=255)
                                                                                                         ttGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAttctcttc                                         >  1:1983307/1‑150 (MQ=255)
                                                                                                         ttGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAttctcttc                                         <  2:4721104/150‑1 (MQ=255)
                                                                                                          tGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAttctcttct                                        <  2:4951693/150‑1 (MQ=255)
                                                                                                          tGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAttctcttct                                        >  2:1363891/1‑150 (MQ=255)
                                                                                                             cgtcTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCt                                     >  1:2176746/1‑150 (MQ=255)
                                                                                                             cgtcTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCt                                     <  1:2190471/150‑1 (MQ=255)
                                                                                                             cgtcTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCt                                     >  1:1734064/1‑150 (MQ=255)
                                                                                                             cgtcTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCt                                     <  1:3050681/150‑1 (MQ=255)
                                                                                                                cTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTCCTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTc                                  >  2:2494639/1‑150 (MQ=255)
                                                                                                                 tGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCt                                 >  2:759622/1‑150 (MQ=255)
                                                                                                                 tGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCt                                 <  1:2693686/150‑1 (MQ=255)
                                                                                                                    gttgttTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCg                              <  1:2346961/150‑1 (MQ=255)
                                                                                                                       gttTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCa                           <  2:4029928/150‑1 (MQ=255)
                                                                                                                       gttTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCa                           <  1:48576/150‑1 (MQ=255)
                                                                                                                       gttTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATGAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCa                           <  1:4736974/150‑1 (MQ=255)
                                                                                                                         tttCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCAcc                         >  1:5045759/1‑150 (MQ=255)
                                                                                                                            cGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCAccgcc                      <  1:1204315/150‑1 (MQ=255)
                                                                                                                            cGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCAccgcc                      <  2:1587012/150‑1 (MQ=255)
                                                                                                                                 aacacaaacTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCa                 >  1:3153563/1‑150 (MQ=255)
                                                                                                                                  acacaaacTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCAc                >  1:4477516/1‑150 (MQ=255)
                                                                                                                                       aacTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATga           >  2:2547931/1‑150 (MQ=255)
                                                                                                                                         cTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATgaga         <  2:1777937/150‑1 (MQ=255)
                                                                                                                                           cgcgTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAgg       >  2:4735912/1‑150 (MQ=255)
                                                                                                                                                gCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCt  <  2:341213/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCT  >  CP009273/302281‑302574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTG  >  CP009273/302279‑302575
                                                                                                                                                     |                                                                                                                                                   
CGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCAAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCT                                                                                                                                                     >  SRR19973063.699884/1‑150 (MQ=60)
 GAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTG                                                                                                                                                    >  SRR19973063.1841104/1‑150 (MQ=60)
  AGGCTTTGCTGATAGATGGTTCCTGTACCAGTGTTTTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGT                                                                                                                                                   <  SRR19973063.1198489/150‑1 (MQ=60)
     CTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCA                                                                                                                                                >  SRR19973063.2443409/1‑150 (MQ=60)
      TTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCAT                                                                                                                                               >  SRR19973063.2166473/1‑150 (MQ=60)
      TTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCAT                                                                                                                                               >  SRR19973063.2166705/1‑150 (MQ=60)
         GCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCG                                                                                                                                            <  SRR19973063.682744/150‑1 (MQ=60)
         GCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCG                                                                                                                                            <  SRR19973063.2584609/150‑1 (MQ=60)
           TGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCC                                                                                                                                          >  SRR19973063.3806767/1‑150 (MQ=60)
               AGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGG                                                                                                                                      <  SRR19973063.1878213/150‑1 (MQ=60)
               AGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGG                                                                                                                                      <  SRR19973063.1878284/150‑1 (MQ=60)
               AGAGGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGG                                                                                                                                      <  SRR19973063.1804337/150‑1 (MQ=60)
                  TGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTG                                                                                                                                   <  SRR19973063.1877030/150‑1 (MQ=60)
                  TGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTAG                                                                                                                                   >  SRR19973063.3906165/1‑150 (MQ=60)
                      TCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTT                                                                                                                               <  SRR19973063.2752511/150‑1 (MQ=60)
                      TCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTT                                                                                                                               <  SRR19973063.3747434/150‑1 (MQ=60)
                           AACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCA                                                                                                                          >  SRR19973063.4453531/1‑150 (MQ=60)
                             CCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACT                                                                                                                        <  SRR19973063.3917877/150‑1 (MQ=60)
                             CCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACT                                                                                                                        <  SRR19973063.3917870/150‑1 (MQ=60)
                              CAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTG                                                                                                                       >  SRR19973063.1968560/1‑150 (MQ=60)
                                   TATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCT                                                                                                                  >  SRR19973063.2618662/1‑150 (MQ=60)
                                   TATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCT                                                                                                                  >  SRR19973063.583914/1‑150 (MQ=60)
                                       TTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGA                                                                                                              <  SRR19973063.1113528/150‑1 (MQ=60)
                                         AAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGC                                                                                                            >  SRR19973063.2915968/1‑150 (MQ=60)
                                          AACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCG                                                                                                           <  SRR19973063.760338/150‑1 (MQ=60)
                                          AACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCG                                                                                                           <  SRR19973063.3248515/150‑1 (MQ=60)
                                          AACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCG                                                                                                           >  SRR19973063.3935710/1‑150 (MQ=60)
                                           ACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGC                                                                                                          >  SRR19973063.2310886/1‑150 (MQ=60)
                                             CGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAG                                                                                                        <  SRR19973063.156143/150‑1 (MQ=60)
                                              GGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGT                                                                                                       >  SRR19973063.4648634/1‑150 (MQ=60)
                                              GGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGT                                                                                                       >  SRR19973063.652238/1‑150 (MQ=60)
                                              GGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGCTGTTTTGCTCACTGCCGCTCTGAGCGCAGT                                                                                                       >  SRR19973063.4648540/1‑150 (MQ=60)
                                               GTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTT                                                                                                      >  SRR19973063.4837245/1‑150 (MQ=60)
                                               GTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTGTTGCTCACTGCCGCTCTGAGCGCAGTT                                                                                                      >  SRR19973063.633197/1‑150 (MQ=60)
                                               GTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTT                                                                                                      <  SRR19973063.3830823/150‑1 (MQ=60)
                                               GTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTT                                                                                                      <  SRR19973063.4308547/150‑1 (MQ=60)
                                                TCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTA                                                                                                     >  SRR19973063.2119095/1‑150 (MQ=60)
                                                TCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTA                                                                                                     <  SRR19973063.2638231/150‑1 (MQ=60)
                                                TCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTA                                                                                                     >  SRR19973063.1413002/1‑150 (MQ=60)
                                                TCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTA                                                                                                     <  SRR19973063.254236/150‑1 (MQ=60)
                                                TCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCATGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTA                                                                                                     <  SRR19973063.123626/150‑1 (MQ=60)
                                                 CCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTAC                                                                                                    >  SRR19973063.3189425/1‑150 (MQ=60)
                                                   AGCGGTTGGCACCGGTCAGGCGCGGATCAAGCGCCCCGCCAGGAGTGACGAAAAATTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCATCTCTGAGCGCAGTTACTG                                                                                                  >  SRR19973063.1273239/1‑150 (MQ=60)
                                                    GCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGG                                                                                                 <  SRR19973063.4392572/150‑1 (MQ=60)
                                                       GTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCA                                                                                              >  SRR19973063.4338761/1‑150 (MQ=60)
                                                          GGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGAT                                                                                           >  SRR19973063.4916885/1‑150 (MQ=60)
                                                          GGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGAT                                                                                           >  SRR19973063.574826/1‑150 (MQ=60)
                                                          GGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGAT                                                                                           >  SRR19973063.1167586/1‑150 (MQ=60)
                                                             ACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAG                                                                                        >  SRR19973063.1429482/1‑150 (MQ=60)
                                                             ACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAG                                                                                        <  SRR19973063.4630787/150‑1 (MQ=60)
                                                             ACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAG                                                                                        <  SRR19973063.4712683/150‑1 (MQ=60)
                                                              CCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGC                                                                                       <  SRR19973063.4127631/150‑1 (MQ=60)
                                                                 GTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCG                                                                                    <  SRR19973063.1111622/150‑1 (MQ=60)
                                                                 GTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCG                                                                                    <  SRR19973063.3180981/150‑1 (MQ=60)
                                                                    AGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTCCTGGCCAGATTAGCGCGAAA                                                                                 >  SRR19973063.2742793/1‑150 (MQ=60)
                                                                    AGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAA                                                                                 >  SRR19973063.4314509/1‑150 (MQ=60)
                                                                    AGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAA                                                                                 >  SRR19973063.2742745/1‑150 (MQ=60)
                                                                    AGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAA                                                                                 >  SRR19973063.1671874/1‑150 (MQ=60)
                                                                        GCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTA                                                                             >  SRR19973063.2367744/1‑150 (MQ=60)
                                                                        GCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTA                                                                             <  SRR19973063.3018832/150‑1 (MQ=60)
                                                                        GCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTA                                                                             <  SRR19973063.4691329/150‑1 (MQ=60)
                                                                         CGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTAT                                                                            <  SRR19973063.4369894/150‑1 (MQ=60)
                                                                            ATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCAT                                                                         <  SRR19973063.1723049/150‑1 (MQ=60)
                                                                             TCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATC                                                                        <  SRR19973063.629806/150‑1 (MQ=60)
                                                                             TCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATC                                                                        <  SRR19973063.3587644/150‑1 (MQ=60)
                                                                             TCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATC                                                                        <  SRR19973063.1930245/150‑1 (MQ=60)
                                                                                   GCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATT                                                                  <  SRR19973063.1986764/150‑1 (MQ=60)
                                                                                   GCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATT                                                                  >  SRR19973063.2271044/1‑150 (MQ=60)
                                                                                    CCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTA                                                                 >  SRR19973063.3742768/1‑150 (MQ=60)
                                                                                       CGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTA                                                              >  SRR19973063.2625883/1‑150 (MQ=60)
                                                                                         CCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCCGTTACTGGCCAGATTAGCGCGACAATTATCATCGTTCTTAGTAGG                                                            >  SRR19973063.895777/1‑150 (MQ=60)
                                                                                         CCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGG                                                            >  SRR19973063.342195/1‑150 (MQ=60)
                                                                                         CCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGG                                                            >  SRR19973063.5081712/1‑150 (MQ=60)
                                                                                          CAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGT                                                           >  SRR19973063.2319157/1‑150 (MQ=60)
                                                                                             GAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTGAGTAGTAGGTTAA                                                        >  SRR19973063.2694159/1‑150 (MQ=60)
                                                                                              AGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAAC                                                       >  SRR19973063.3047965/1‑150 (MQ=60)
                                                                                                   CGAAAAAGTTGTCGTCTGATTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGACGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCA                                                  <  SRR19973063.1315587/150‑1 (MQ=60)
                                                                                                   CGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCA                                                  >  SRR19973063.1818449/1‑150 (MQ=60)
                                                                                                     AAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATT                                                >  SRR19973063.3158517/1‑150 (MQ=60)
                                                                                                     AAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATT                                                >  SRR19973063.361879/1‑150 (MQ=60)
                                                                                                     AAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATT                                                >  SRR19973063.361874/1‑150 (MQ=60)
                                                                                                      AAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGGTATTAGTAGGTTAACTCTCATTA                                               >  SRR19973063.3950215/1‑150 (MQ=60)
                                                                                                       AAAGTTTTAGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTTCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTATGTGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCT                                              <  SRR19973063.4740870/150‑1 (MQ=60)
                                                                                                       AAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCT                                              <  SRR19973063.831061/150‑1 (MQ=60)
                                                                                                        AAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTC                                             <  SRR19973063.160122/150‑1 (MQ=60)
                                                                                                        AAGGTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTC                                             <  SRR19973063.3984783/150‑1 (MQ=60)
                                                                                                           TTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTC                                          >  SRR19973063.1989507/1‑150 (MQ=60)
                                                                                                            TGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCT                                         <  SRR19973063.4369727/150‑1 (MQ=60)
                                                                                                            TGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCT                                         <  SRR19973063.1788216/150‑1 (MQ=60)
                                                                                                            TGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGAGCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCT                                         <  SRR19973063.2654208/150‑1 (MQ=60)
                                                                                                               CGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCT                                      <  SRR19973063.1619508/150‑1 (MQ=60)
                                                                                                               CGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCT                                      >  SRR19973063.1739345/1‑150 (MQ=60)
                                                                                                               CGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCT                                      <  SRR19973063.3060453/150‑1 (MQ=60)
                                                                                                               CGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCT                                      >  SRR19973063.2183558/1‑150 (MQ=60)
                                                                                                               CGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCT                                      <  SRR19973063.2197333/150‑1 (MQ=60)
                                                                                                               CGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCT                                      <  SRR19973063.2197347/150‑1 (MQ=60)
                                                                                                                 TCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGT                                    >  SRR19973063.3341875/1‑150 (MQ=60)
                                                                                                                   TGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCT                                  >  SRR19973063.2002399/1‑150 (MQ=60)
                                                                                                                   TGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCT                                  >  SRR19973063.4573041/1‑150 (MQ=60)
                                                                                                                   TGAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCT                                  <  SRR19973063.2702367/150‑1 (MQ=60)
                                                                                                                    GAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTG                                 >  SRR19973063.4686499/1‑150 (MQ=60)
                                                                                                                    GAGTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTG                                 <  SRR19973063.5045024/150‑1 (MQ=60)
                                                                                                                      GTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCG                               <  SRR19973063.2354449/150‑1 (MQ=60)
                                                                                                                      GTTGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCG                               <  SRR19973063.2354469/150‑1 (MQ=60)
                                                                                                                        TGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTC                             >  SRR19973063.744620/1‑150 (MQ=60)
                                                                                                                        TGTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTC                             <  SRR19973063.3580385/150‑1 (MQ=60)
                                                                                                                         GTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCA                            <  SRR19973063.48748/150‑1 (MQ=60)
                                                                                                                         GTTTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATGAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCA                            <  SRR19973063.4752064/150‑1 (MQ=60)
                                                                                                                           TTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACC                          >  SRR19973063.5062015/1‑150 (MQ=60)
                                                                                                                           TTTCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACC                          >  SRR19973063.5062043/1‑150 (MQ=60)
                                                                                                                             TCGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGC                        >  SRR19973063.1395817/1‑150 (MQ=60)
                                                                                                                              CGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCC                       <  SRR19973063.1207930/150‑1 (MQ=60)
                                                                                                                              CGACAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCC                       <  SRR19973063.1198727/150‑1 (MQ=60)
                                                                                                                                 CAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCC                    <  SRR19973063.961241/150‑1 (MQ=60)
                                                                                                                                 CAAACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCC                    <  SRR19973063.961437/150‑1 (MQ=60)
                                                                                                                                   AACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCA                  >  SRR19973063.3163703/1‑150 (MQ=60)
                                                                                                                                    ACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCAC                 >  SRR19973063.4491946/1‑150 (MQ=60)
                                                                                                                                    ACACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCAC                 >  SRR19973063.4491873/1‑150 (MQ=60)
                                                                                                                                     CACAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACG                >  SRR19973063.5147186/1‑150 (MQ=60)
                                                                                                                                     CACAAACTCGCGTGCCTGTGCATCCGCCCAGGGTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCAATCTCTTCTCCTGTCTGCGACACCGCCGCCCACG                >  SRR19973063.5147185/1‑150 (MQ=60)
                                                                                                                                       CAAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGAT              <  SRR19973063.1877423/150‑1 (MQ=60)
                                                                                                                                        AAACTCGCGTGCCTGTGCATCCGCCTAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATG             >  SRR19973063.2258223/1‑150 (MQ=60)
                                                                                                                                        AAACTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATG             >  SRR19973063.4641903/1‑150 (MQ=60)
                                                                                                                                           CTCGCGTGCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGA          <  SRR19973063.841336/150‑1 (MQ=60)
                                                                                                                                                  GCCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCT   >  SRR19973063.2810147/1‑150 (MQ=60)
                                                                                                                                                   CCTGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTG  <  SRR19973063.2529222/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
CGAGGCTTTGCTGATAGATGGTTCCTGAACCAGTGTATTTTAAACCGGTCCAGCGGTTGGCACCGGTCAGGCGCGGATCCAGCGCCCCGCCAGGAGTGACGAAAAAGTTGTCGTCTGAGTTGTTTTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTG  >  CP009273/302279‑302575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑