Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,038,316 G→T 100% R451S (CGT→AGT)  etk ← tyrosine‑protein kinase, role in O‑antigen capsule formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,038,3160GT97.8% 583.0 / 3.4 184R451S (CGT→AGT) etktyrosine‑protein kinase, role in O‑antigen capsule formation
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base T (99/81);  total (99/85)
Fisher's exact test for biased strand distribution p-value = 4.38e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 186 total aligned reads displayed.

TATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACGACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGG  >  CP009273/1038172‑1038459
                                                                                                                                                |                                                                                                                                               
tatTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCactacg                                                                                                                                              >  1:3017380/1‑148 (MQ=255)
tatTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCactacg                                                                                                                                              >  1:2351985/1‑148 (MQ=255)
  tttttAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCa                                                                                                                                            >  1:2969703/1‑148 (MQ=255)
     ttAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACa                                                                                                                                         >  2:424152/1‑148 (MQ=255)
        gTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCg                                                                                                                                      >  2:3300285/1‑148 (MQ=255)
        gTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCg                                                                                                                                      >  2:3300323/1‑148 (MQ=255)
        gTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCg                                                                                                                                      >  1:824549/1‑148 (MQ=255)
        gTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCg                                                                                                                                      >  1:824751/1‑148 (MQ=255)
          aCGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCa                                                                                                                                    <  1:3259350/148‑1 (MQ=255)
              tGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTGATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCAcgcg                                                                                                                                <  2:3288356/148‑1 (MQ=255)
               ggCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACgcgc                                                                                                                               >  2:982449/1‑148 (MQ=255)
                gcgcTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGcc                                                                                                                              >  1:266833/1‑148 (MQ=255)
                   ctgctgATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCTCCAGc                                                                                                                           >  2:3143756/1‑148 (MQ=255)
                   ctgctgATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGc                                                                                                                           <  2:2058008/148‑1 (MQ=255)
                   ctgctgATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGc                                                                                                                           <  1:2651830/148‑1 (MQ=255)
                   ctgctgATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGc                                                                                                                           <  1:210627/148‑1 (MQ=255)
                    tgctgATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCa                                                                                                                          <  2:1018618/148‑1 (MQ=255)
                    tgctgATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCa                                                                                                                          >  2:1893377/1‑148 (MQ=255)
                    tgctgATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCa                                                                                                                          <  1:1503835/148‑1 (MQ=255)
                     gctgATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGTAc                                                                                                                         >  2:2915302/1‑148 (MQ=255)
                     gctgATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCAc                                                                                                                         >  1:3041281/1‑148 (MQ=255)
                        gATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGc                                                                                                                      >  2:2402329/1‑148 (MQ=255)
                         aTTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCa                                                                                                                     <  2:68179/148‑1 (MQ=255)
                         aTTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCa                                                                                                                     <  1:2926985/148‑1 (MQ=255)
                          ttAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAc                                                                                                                    >  1:2304001/1‑148 (MQ=255)
                          ttAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAc                                                                                                                    >  1:1193145/1‑148 (MQ=255)
                           tAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAcc                                                                                                                   <  2:2111530/148‑1 (MQ=255)
                           tAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAcc                                                                                                                   >  2:268323/1‑148 (MQ=255)
                             gAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCca                                                                                                                 <  1:3473070/148‑1 (MQ=255)
                             gAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCca                                                                                                                 <  2:292842/148‑1 (MQ=255)
                              aaaaTAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCcac                                                                                                                >  1:3465693/1‑148 (MQ=255)
                              aaaaTAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCcac                                                                                                                <  1:1602089/148‑1 (MQ=255)
                               aaataaatTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCcaca                                                                                                               >  1:2415766/1‑148 (MQ=255)
                                aataaatTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAg                                                                                                              >  1:2901658/1‑148 (MQ=255)
                                aataaatTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAg                                                                                                              >  2:442789/1‑148 (MQ=255)
                                aataaatTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAg                                                                                                              >  2:443164/1‑148 (MQ=255)
                                  taaatTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGaa                                                                                                            <  2:2543503/148‑1 (MQ=255)
                                  taaatTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGaa                                                                                                            >  2:1419184/1‑148 (MQ=255)
                                  taaatTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACGACGCAACATCGCACGCGCCAGCACGGCACCCACAGaa                                                                                                            <  2:372602/148‑1 (MQ=255)
                                   aaatTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGaaa                                                                                                           >  2:2905005/1‑148 (MQ=255)
                                     atTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATa                                                                                                         >  1:759533/1‑148 (MQ=255)
                                     atTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATa                                                                                                         <  2:2210824/148‑1 (MQ=255)
                                     atTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATa                                                                                                         >  2:3497190/1‑148 (MQ=255)
                                          tCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAg                                                                                                    >  1:1298086/1‑148 (MQ=255)
                                              aCGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCa                                                                                                <  2:3076021/148‑1 (MQ=255)
                                               cGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCaa                                                                                               >  1:408320/1‑148 (MQ=255)
                                                   gACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAAt                                                                                           <  2:1944565/148‑1 (MQ=255)
                                                    aCGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATa                                                                                          <  1:1413756/148‑1 (MQ=255)
                                                       ggTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATaaaa                                                                                       <  1:2110172/148‑1 (MQ=255)
                                                        gTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAAc                                                                                      <  2:2816154/148‑1 (MQ=255)
                                                             tttATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGc                                                                                 >  2:339207/1‑148 (MQ=255)
                                                              ttATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGca                                                                                >  1:2109355/1‑148 (MQ=255)
                                                                  ccAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGcaccac                                                                            >  1:2156780/1‑148 (MQ=255)
                                                                   cAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACg                                                                           <  2:824549/148‑1 (MQ=255)
                                                                   cAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACg                                                                           <  2:1179696/148‑1 (MQ=255)
                                                                   cAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACg                                                                           <  2:824751/148‑1 (MQ=255)
                                                                     gCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGtt                                                                         <  1:68198/148‑1 (MQ=255)
                                                                       cACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCa                                                                       >  1:1043324/1‑148 (MQ=255)
                                                                        aCTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCaa                                                                      <  2:2539356/148‑1 (MQ=255)
                                                                        aCTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCaa                                                                      >  2:1709387/1‑148 (MQ=255)
                                                                        aCTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCaa                                                                      <  1:2999171/148‑1 (MQ=255)
                                                                           cGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGc                                                                   <  1:887091/148‑1 (MQ=255)
                                                                           cGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGc                                                                   <  1:2532153/148‑1 (MQ=255)
                                                                             gACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCtt                                                                 >  2:923623/1‑148 (MQ=255)
                                                                             gACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGATTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCtt                                                                 >  2:721053/1‑148 (MQ=255)
                                                                                 ttGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCtttttt                                                             >  1:1192967/1‑148 (MQ=255)
                                                                                   gggATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCg                                                           <  1:3323659/148‑1 (MQ=255)
                                                                                   gggATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCg                                                           <  1:3323649/148‑1 (MQ=255)
                                                                                    ggATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACGACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCgg                                                          <  2:1665951/148‑1 (MQ=255)
                                                                                       tAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGttt                                                       >  2:1385074/1‑148 (MQ=255)
                                                                                       tAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGACAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTAGGttt                                                       >  2:1384953/1‑148 (MQ=255)
                                                                                              aTAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGc                                                <  1:3300323/148‑1 (MQ=255)
                                                                                                aaaCGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTg                                              >  2:1798365/1‑148 (MQ=255)
                                                                                                   cGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCgg                                           <  1:2015534/148‑1 (MQ=255)
                                                                                                   cGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCgg                                           >  2:1792469/1‑148 (MQ=255)
                                                                                                    gCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGc                                          <  2:1559534/148‑1 (MQ=255)
                                                                                                      tGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTg                                        >  1:1289935/1‑148 (MQ=255)
                                                                                                        aTGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACTGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAg                                      >  2:2800306/1‑148 (MQ=255)
                                                                                                           ccGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGa                                   >  1:1673978/1‑148 (MQ=255)
                                                                                                           ccGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGa                                   <  1:2437962/148‑1 (MQ=255)
                                                                                                              tGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTg                                >  2:2208970/1‑148 (MQ=255)
                                                                                                              tGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTg                                >  1:1567032/1‑148 (MQ=255)
                                                                                                                 tctTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCg                             <  2:1467380/148‑1 (MQ=255)
                                                                                                                   ttCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCggg                           <  1:3204325/148‑1 (MQ=255)
                                                                                                                      cAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCg                        >  2:43261/1‑148 (MQ=255)
                                                                                                                         ttgttCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATa                     >  1:682359/1‑148 (MQ=255)
                                                                                                                         ttgttCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATa                     <  2:2156780/148‑1 (MQ=255)
                                                                                                                         ttggtCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATa                     <  1:2187194/148‑1 (MQ=255)
                                                                                                                           gttCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAAt                   <  1:655609/148‑1 (MQ=255)
                                                                                                                           gttCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAAt                   <  1:1893377/148‑1 (MQ=255)
                                                                                                                            ttCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATc                  <  2:2193524/148‑1 (MQ=255)
                                                                                                                               cGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGc               <  2:2969703/148‑1 (MQ=255)
                                                                                                                                  gtcTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACg            >  1:1147998/3‑148 (MQ=255)
                                                                                                                                  ggCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTATTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACg            <  2:2415766/148‑1 (MQ=255)
                                                                                                                                         acacCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCaa     <  1:43271/148‑1 (MQ=255)
                                                                                                                                          caccacTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAAt    >  2:2081276/1‑148 (MQ=255)
                                                                                                                                          caccacTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAAt    >  2:287509/1‑148 (MQ=255)
                                                                                                                                           accacTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATg   >  1:1082171/1‑148 (MQ=255)
                                                                                                                                           accacTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATg   <  2:2351985/148‑1 (MQ=255)
                                                                                                                                            ccacTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATgg  <  2:2714588/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
TATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACGACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGG  >  CP009273/1038172‑1038459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACGACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCACTGG  >  CP009273/1038168‑1038465
                                                                                                                                                    |                                                                                                                                                     
GGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTA                                                                                                                                                      <  SRR19973067.3093718/150‑1 (MQ=60)
  GATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACG                                                                                                                                                    >  SRR19973067.2367337/1‑150 (MQ=60)
  GATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACG                                                                                                                                                    <  SRR19973067.3311878/150‑1 (MQ=60)
  GATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACG                                                                                                                                                    >  SRR19973067.3036590/1‑150 (MQ=60)
    TATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCA                                                                                                                                                  >  SRR19973067.2988443/1‑150 (MQ=60)
     ATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAA                                                                                                                                                 >  SRR19973067.2128432/1‑150 (MQ=60)
          TAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCG                                                                                                                                            >  SRR19973067.830384/1‑150 (MQ=60)
          TAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCG                                                                                                                                            >  SRR19973067.830201/1‑150 (MQ=60)
          TAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCG                                                                                                                                            >  SRR19973067.830181/1‑150 (MQ=60)
           AGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGC                                                                                                                                           >  SRR19973067.2208159/1‑150 (MQ=60)
              ACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACG                                                                                                                                        <  SRR19973067.3279713/150‑1 (MQ=60)
                  TGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCC                                                                                                                                    >  SRR19973067.268634/1‑150 (MQ=60)
                       CTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCAC                                                                                                                               >  SRR19973067.3060654/1‑150 (MQ=60)
                       CTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCAC                                                                                                                               <  SRR19973067.2668924/150‑1 (MQ=60)
                       CTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCAC                                                                                                                               <  SRR19973067.2668925/150‑1 (MQ=60)
                       CTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCAC                                                                                                                               <  SRR19973067.212199/150‑1 (MQ=60)
                        TGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACG                                                                                                                              <  SRR19973067.1513742/150‑1 (MQ=60)
                            GATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAC                                                                                                                          >  SRR19973067.1200977/1‑150 (MQ=60)
                            GATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAC                                                                                                                          >  SRR19973067.2319740/1‑150 (MQ=60)
                            GATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAC                                                                                                                          >  SRR19973067.1200955/1‑150 (MQ=60)
                            GATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAC                                                                                                                          >  SRR19973067.2319183/1‑150 (MQ=60)
                             ATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACC                                                                                                                         >  SRR19973067.764581/1‑150 (MQ=60)
                             ATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACC                                                                                                                         <  SRR19973067.2945584/150‑1 (MQ=60)
                             ATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACC                                                                                                                         <  SRR19973067.1163867/150‑1 (MQ=60)
                               TAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCATCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCA                                                                                                                       >  SRR19973067.3249211/1‑150 (MQ=60)
                                AGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCAC                                                                                                                      >  SRR19973067.3490839/1‑150 (MQ=60)
                                AGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCAC                                                                                                                      >  SRR19973067.3487722/1‑150 (MQ=60)
                                 GAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACA                                                                                                                     <  SRR19973067.3495132/150‑1 (MQ=60)
                                 GAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACA                                                                                                                     >  SRR19973067.2431588/1‑150 (MQ=60)
                                  AAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAG                                                                                                                    >  SRR19973067.2920162/1‑150 (MQ=60)
                                  AAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAG                                                                                                                    >  SRR19973067.3129425/1‑150 (MQ=60)
                                  AAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAG                                                                                                                    >  SRR19973067.2978899/1‑150 (MQ=60)
                                  AAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAG                                                                                                                    <  SRR19973067.1613087/150‑1 (MQ=60)
                                    AATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAA                                                                                                                  >  SRR19973067.872940/1‑150 (MQ=60)
                                       AAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATA                                                                                                               >  SRR19973067.764849/1‑150 (MQ=60)
                                       AAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATA                                                                                                               >  SRR19973067.764807/1‑150 (MQ=60)
                                           TTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACA                                                                                                           <  SRR19973067.1963828/150‑1 (MQ=60)
                                            TTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAG                                                                                                          >  SRR19973067.1306951/1‑150 (MQ=60)
                                               CTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCC                                                                                                       <  SRR19973067.509484/150‑1 (MQ=60)
                                                 TACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAA                                                                                                     >  SRR19973067.411167/1‑150 (MQ=60)
                                                        ACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAA                                                                                              <  SRR19973067.2395170/150‑1 (MQ=60)
                                                        ACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAA                                                                                              <  SRR19973067.1423322/150‑1 (MQ=60)
                                                           GGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACC                                                                                           <  SRR19973067.2124198/150‑1 (MQ=60)
                                                           GGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACC                                                                                           <  SRR19973067.2124138/150‑1 (MQ=60)
                                                            GTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCA                                                                                          <  SRR19973067.2872832/150‑1 (MQ=60)
                                                               CGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGC                                                                                       >  SRR19973067.1384313/1‑150 (MQ=60)
                                                                GTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCA                                                                                      >  SRR19973067.2123376/1‑150 (MQ=60)
                                                                    ATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCAC                                                                                  >  SRR19973067.2171204/1‑150 (MQ=60)
                                                                        AGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTC                                                                              <  SRR19973067.3375927/150‑1 (MQ=60)
                                                                         GCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCA                                                                             <  SRR19973067.69007/150‑1 (MQ=60)
                                                                         GCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCA                                                                             >  SRR19973067.1050496/1‑150 (MQ=60)
                                                                            ACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACG                                                                          <  SRR19973067.3018203/150‑1 (MQ=60)
                                                                               CGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTT                                                                       <  SRR19973067.94583/150‑1 (MQ=60)
                                                                               CGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTT                                                                       <  SRR19973067.892978/150‑1 (MQ=60)
                                                                               CGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTT                                                                       <  SRR19973067.1143603/150‑1 (MQ=60)
                                                                               CGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTT                                                                       <  SRR19973067.2548741/150‑1 (MQ=60)
                                                                                 GACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTT                                                                     >  SRR19973067.2177863/1‑150 (MQ=60)
                                                                                   CATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTT                                                                   >  SRR19973067.1200798/1‑150 (MQ=60)
                                                                                   CATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTT                                                                   >  SRR19973067.174825/1‑150 (MQ=60)
                                                                                    ATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTC                                                                  >  SRR19973067.2925981/1‑150 (MQ=60)
                                                                                       GGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGT                                                               <  SRR19973067.3344669/150‑1 (MQ=60)
                                                                                       GGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGT                                                               <  SRR19973067.3344679/150‑1 (MQ=60)
                                                                                         GATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTT                                                             >  SRR19973067.1553605/1‑150 (MQ=60)
                                                                                              TGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTG                                                        <  SRR19973067.304819/150‑1 (MQ=60)
                                                                                                  ATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTG                                                    <  SRR19973067.3321074/150‑1 (MQ=60)
                                                                                                  ATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTG                                                    <  SRR19973067.3321112/150‑1 (MQ=60)
                                                                                                   TAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGC                                                   >  SRR19973067.2094792/1‑150 (MQ=60)
                                                                                                       CGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCT                                               <  SRR19973067.3034412/150‑1 (MQ=60)
                                                                                                       CGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCT                                               >  SRR19973067.3505702/1‑150 (MQ=60)
                                                                                                       CGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCT                                               <  SRR19973067.2029085/150‑1 (MQ=60)
                                                                                                        GCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTG                                              >  SRR19973067.1298740/1‑150 (MQ=60)
                                                                                                        GCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTG                                              >  SRR19973067.1298745/1‑150 (MQ=60)
                                                                                                             TGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGA                                         >  SRR19973067.1685519/1‑150 (MQ=60)
                                                                                                               CCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACT                                       <  SRR19973067.2453973/150‑1 (MQ=60)
                                                                                                                CGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTG                                      >  SRR19973067.2590225/1‑150 (MQ=60)
                                                                                                                CGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTG                                      >  SRR19973067.1577752/1‑150 (MQ=60)
                                                                                                                CGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTG                                      >  SRR19973067.1581731/1‑150 (MQ=60)
                                                                                                                       TTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTC                               <  SRR19973067.3224525/150‑1 (MQ=60)
                                                                                                                           AGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATA                           >  SRR19973067.249717/1‑150 (MQ=60)
                                                                                                                           AGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATA                           >  SRR19973067.687048/1‑150 (MQ=60)
                                                                                                                             TTGGTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAAT                         <  SRR19973067.2201802/150‑1 (MQ=60)
                                                                                                                               GTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCC                       <  SRR19973067.660270/150‑1 (MQ=60)
                                                                                                                               GTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCC                       <  SRR19973067.660063/150‑1 (MQ=60)
                                                                                                                               GTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCC                       <  SRR19973067.1642203/150‑1 (MQ=60)
                                                                                                                               GTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCC                       <  SRR19973067.1906298/150‑1 (MQ=60)
                                                                                                                                    GGGTCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACG                  >  SRR19973067.1155689/1‑150 (MQ=60)
                                                                                                                                      GGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTT                <  SRR19973067.332941/150‑1 (MQ=60)
                                                                                                                                            TACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAAT          <  SRR19973067.1242401/150‑1 (MQ=60)
                                                                                                                                             ACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCACGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATG         <  SRR19973067.43866/150‑1 (MQ=60)
                                                                                                                                             ACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATG         <  SRR19973067.43855/150‑1 (MQ=60)
                                                                                                                                             ACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATG         >  SRR19973067.1089575/1‑150 (MQ=60)
                                                                                                                                                    TACGCAACATCGCACGCGCCAGTACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCACTGG  <  SRR19973067.2933857/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACGACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCACTGG  >  CP009273/1038168‑1038465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑