Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,038,316 G→T 100% R451S (CGT→AGT)  etk ← tyrosine‑protein kinase, role in O‑antigen capsule formation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,038,3160GT99.6% 825.7 / ‑4.4 252R451S (CGT→AGT) etktyrosine‑protein kinase, role in O‑antigen capsule formation
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (123/128);  total (123/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 258 total aligned reads displayed.

GGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACGACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCAC  >  CP009273/1038169‑1038462
                                                                                                                                                   |                                                                                                                                                  
  atatTTTTAGTACGATGGCGCTGTTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGc                                                                                                                                                <  1:756359/150‑1 (MQ=255)
    atTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCaa                                                                                                                                              >  2:3833615/1‑150 (MQ=255)
    atTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCaa                                                                                                                                              >  1:4959859/1‑150 (MQ=255)
      ttttAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACa                                                                                                                                            <  1:1941096/150‑1 (MQ=255)
       tttAGTACGATNGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCAAGTTGTTCCGGGGCTTCTACACCACTACGCAACAt                                                                                                                                           >  1:2758289/1‑150 (MQ=255)
       tttAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACAt                                                                                                                                           <  2:3546452/150‑1 (MQ=255)
         tAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCg                                                                                                                                         >  2:3575554/1‑150 (MQ=255)
           gTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCa                                                                                                                                       >  2:4612196/1‑150 (MQ=255)
              cGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCAcgc                                                                                                                                    <  2:2650989/150‑1 (MQ=255)
                 tGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGcc                                                                                                                                 >  2:1720486/1‑150 (MQ=255)
                    cgcTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGc                                                                                                                              >  1:3935504/1‑150 (MQ=255)
                     gctgctGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCa                                                                                                                             <  2:2214715/150‑1 (MQ=255)
                       tgctgATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACg                                                                                                                           <  1:5031597/150‑1 (MQ=255)
                          tgATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCa                                                                                                                        >  1:4742288/1‑150 (MQ=255)
                           gATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAc                                                                                                                       >  1:805252/1‑150 (MQ=255)
                           gATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAc                                                                                                                       >  2:972990/1‑150 (MQ=255)
                             ttAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAccc                                                                                                                     >  1:102949/1‑150 (MQ=255)
                             ttAGAAAATAAATTGTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAccc                                                                                                                     <  2:4826638/150‑1 (MQ=255)
                              tAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCca                                                                                                                    <  1:4107031/150‑1 (MQ=255)
                                gAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCcaca                                                                                                                  <  2:3378181/150‑1 (MQ=255)
                                gAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCcaca                                                                                                                  >  2:1990927/1‑150 (MQ=255)
                                gAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCcaca                                                                                                                  >  1:1804664/1‑150 (MQ=255)
                                 aaaaTAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAg                                                                                                                 <  1:4504993/150‑1 (MQ=255)
                                   aataaatTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGaa                                                                                                               >  1:1248016/1‑150 (MQ=255)
                                    ataaatTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGaaa                                                                                                              >  1:1653979/1‑150 (MQ=255)
                                     taaatTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGAGCCAGCACGGCACCCACAGAAAt                                                                                                             <  1:2917823/150‑1 (MQ=255)
                                       aatTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATaa                                                                                                           >  2:3301516/1‑150 (MQ=255)
                                        atTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATaaa                                                                                                          >  2:62523/1‑150 (MQ=255)
                                        atTTTTCTTAAGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATaaa                                                                                                          >  1:102092/1‑150 (MQ=255)
                                         tttttCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAAc                                                                                                         <  2:1197897/150‑1 (MQ=255)
                                         tttttCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAAc                                                                                                         <  2:1535960/150‑1 (MQ=255)
                                          ttttCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACa                                                                                                        >  2:454905/1‑150 (MQ=255)
                                          ttttCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACa                                                                                                        >  1:5268036/1‑150 (MQ=255)
                                          ttttCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACa                                                                                                        <  2:1845545/150‑1 (MQ=255)
                                           tttCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAg                                                                                                       <  2:3618697/150‑1 (MQ=255)
                                             tCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGc                                                                                                     <  1:4931061/150‑1 (MQ=255)
                                             tCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGc                                                                                                     >  2:2022159/1‑150 (MQ=255)
                                              cTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGcc                                                                                                    >  1:3516847/1‑150 (MQ=255)
                                              cTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGcc                                                                                                    >  1:650463/1‑150 (MQ=255)
                                              cTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGcc                                                                                                    <  1:2794903/150‑1 (MQ=255)
                                               ttACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCa                                                                                                   <  1:52228/150‑1 (MQ=255)
                                                     aGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATa                                                                                             <  1:3096310/150‑1 (MQ=255)
                                                     aGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATa                                                                                             <  2:403194/150‑1 (MQ=255)
                                                      gACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCTCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATaa                                                                                            >  1:1457300/1‑150 (MQ=255)
                                                      gACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCAGGGGCTTCTACACCTCTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATaa                                                                                            <  2:2980295/150‑1 (MQ=255)
                                                       aCGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATaaa                                                                                           <  2:3243711/150‑1 (MQ=255)
                                                        cGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATaaaa                                                                                          >  2:1332825/1‑150 (MQ=255)
                                                          ggTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAAcc                                                                                        >  1:4171643/1‑150 (MQ=255)
                                                             gCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAg                                                                                     <  2:2305568/150‑1 (MQ=255)
                                                             gCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAg                                                                                     <  1:4335087/150‑1 (MQ=255)
                                                              cGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGc                                                                                    >  2:5165751/1‑150 (MQ=255)
                                                              cGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGc                                                                                    >  1:1243202/1‑150 (MQ=255)
                                                               gTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGca                                                                                   >  2:4155214/1‑150 (MQ=255)
                                                               gTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGca                                                                                   >  2:4011731/1‑150 (MQ=255)
                                                                  tATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGcacca                                                                                >  2:4251217/1‑150 (MQ=255)
                                                                   aTCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGcaccac                                                                               <  2:1292037/150‑1 (MQ=255)
                                                                   aTCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGcaccac                                                                               <  1:3159043/150‑1 (MQ=255)
                                                                    tCCAGCCTCTCGGTCATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAAAATCGCACGCGCAAGCAAGTCACCAACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACg                                                                              <  1:3771285/150‑1 (MQ=255)
                                                                       aGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTc                                                                           >  1:286921/1‑150 (MQ=255)
                                                                       aGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTc                                                                           >  1:373395/1‑150 (MQ=255)
                                                                          cACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAAc                                                                        <  1:4579022/150‑1 (MQ=255)
                                                                          cACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAAc                                                                        <  2:3090745/150‑1 (MQ=255)
                                                                               ggACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCttt                                                                   <  1:305441/150‑1 (MQ=255)
                                                                               ggACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCttt                                                                   <  1:2560658/150‑1 (MQ=255)
                                                                               ggACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCatt                                                                   >  2:3033365/1‑150 (MQ=255)
                                                                                gACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCtttt                                                                  <  1:2406642/150‑1 (MQ=255)
                                                                                  cATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCtttttt                                                                >  2:2453784/1‑150 (MQ=255)
                                                                                      gggATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGt                                                            >  1:399342/1‑150 (MQ=255)
                                                                                        gATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGTGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGACAAGAATAAAACCAAGCACCACGATCAACGCTTTTTTCGGatt                                                          >  2:536272/1‑150 (MQ=255)
                                                                                          tAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCTCGTCGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCTACGCTTTTTTCGGTTTCa                                                        <  2:1588591/150‑1 (MQ=255)
                                                                                           aGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCAc                                                       <  1:3526137/150‑1 (MQ=255)
                                                                                              ggCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTgg                                                    <  1:2627320/150‑1 (MQ=255)
                                                                                                  tAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGc                                                <  1:686206/150‑1 (MQ=255)
                                                                                                   aaaCGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCg                                               <  1:1592480/150‑1 (MQ=255)
                                                                                                       gCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTg                                           >  1:4790066/1‑150 (MQ=255)
                                                                                                        cTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGa                                          <  2:5005428/150‑1 (MQ=255)
                                                                                                         tGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAg                                         >  1:1636778/1‑150 (MQ=255)
                                                                                                          gATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGt                                        <  1:3213518/150‑1 (MQ=255)
                                                                                                             gCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGAc                                     <  2:4959943/150‑1 (MQ=255)
                                                                                                              ccGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACt                                    >  2:3193166/1‑150 (MQ=255)
                                                                                                               cGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTg                                   >  1:4995511/1‑150 (MQ=255)
                                                                                                                 tGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGcc                                 >  2:5258954/1‑150 (MQ=255)
                                                                                                                      ttCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTc                            <  2:785776/150‑1 (MQ=255)
                                                                                                                         cAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGAt                         <  2:4466022/150‑1 (MQ=255)
                                                                                                                         cAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGAt                         <  2:1429601/150‑1 (MQ=255)
                                                                                                                           gttgttCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATaa                       >  1:1404009/1‑150 (MQ=255)
                                                                                                                            ttgttCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAAt                      <  2:4441736/150‑1 (MQ=255)
                                                                                                                             tgttCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATc                     <  1:2163353/150‑1 (MQ=255)
                                                                                                                             tgttCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATc                     <  2:4958942/150‑1 (MQ=255)
                                                                                                                              gttCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATcc                    <  2:886931/150‑1 (MQ=255)
                                                                                                                              gttCCGGGGATTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATACAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATcc                    <  2:1051792/150‑1 (MQ=255)
                                                                                                                                tCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGc                  >  2:593788/1‑150 (MQ=255)
                                                                                                                                     ggCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGtt             <  2:4205031/150‑1 (MQ=255)
                                                                                                                                      gCTTCTACACCACTACGCAACATCGCACGCTCCAGCACGGCACCCACAGAAATAAACATGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTTCCTGGTCGATAATCCGCACGTTa            >  2:1386553/1‑150 (MQ=255)
                                                                                                                                      gCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTa            <  1:3301516/150‑1 (MQ=255)
                                                                                                                                             caccacTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATgg     <  2:2698223/150‑1 (MQ=255)
                                                                                                                                             caccacTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATgg     <  1:4347979/150‑1 (MQ=255)
                                                                                                                                              accacTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGc    >  1:2083104/1‑150 (MQ=255)
                                                                                                                                                cacTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCAc  <  1:2852264/150‑1 (MQ=255)
                                                                                                                                                cacTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCAc  >  2:1871193/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACGACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCAC  >  CP009273/1038169‑1038462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACGACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCACTG  >  CP009273/1038167‑1038464
                                                                                                                                                     |                                                                                                                                                    
GGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACT                                                                                                                                                      <  SRR19973062.5297595/150‑1 (MQ=60)
 GGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTA                                                                                                                                                     >  SRR19973062.4045525/1‑150 (MQ=60)
  GGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTAC                                                                                                                                                    <  SRR19973062.1658613/150‑1 (MQ=60)
  GGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTAC                                                                                                                                                    <  SRR19973062.1999744/150‑1 (MQ=60)
    ATATTTTTAGTACGATGGCGCTGTTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGC                                                                                                                                                  <  SRR19973062.759775/150‑1 (MQ=60)
    ATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGC                                                                                                                                                  >  SRR19973062.3205223/1‑150 (MQ=60)
      ATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAA                                                                                                                                                >  SRR19973062.4985893/1‑150 (MQ=60)
      ATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAA                                                                                                                                                >  SRR19973062.4985930/1‑150 (MQ=60)
      ATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAA                                                                                                                                                >  SRR19973062.4986016/1‑150 (MQ=60)
       TTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAAC                                                                                                                                               >  SRR19973062.3577392/1‑150 (MQ=60)
        TTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACA                                                                                                                                              <  SRR19973062.1951056/150‑1 (MQ=60)
         TTTAGTACGATNGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCAAGTTGTTCCGGGGCTTCTACACCACTACGCAACAT                                                                                                                                             >  SRR19973062.2772858/1‑150 (MQ=60)
          TTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATC                                                                                                                                            <  SRR19973062.4811944/150‑1 (MQ=60)
          TTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATC                                                                                                                                            <  SRR19973062.288093/150‑1 (MQ=60)
          TTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATC                                                                                                                                            <  SRR19973062.288029/150‑1 (MQ=60)
           TAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCG                                                                                                                                           <  SRR19973062.2681491/150‑1 (MQ=60)
           TAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCG                                                                                                                                           >  SRR19973062.1850874/1‑150 (MQ=60)
                CGAGGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGC                                                                                                                                      <  SRR19973062.5282261/150‑1 (MQ=60)
                 GATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCG                                                                                                                                     >  SRR19973062.3766816/1‑150 (MQ=60)
                      CGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGC                                                                                                                                >  SRR19973062.3955494/1‑150 (MQ=60)
                        CTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCAC                                                                                                                              >  SRR19973062.890728/1‑150 (MQ=60)
                        CTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCAAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCTTGCTCTACCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCAC                                                                                                                              >  SRR19973062.789278/1‑150 (MQ=60)
                         TGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACG                                                                                                                             <  SRR19973062.5057942/150‑1 (MQ=60)
                         TGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACG                                                                                                                             <  SRR19973062.5058263/150‑1 (MQ=60)
                           CTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGC                                                                                                                           <  SRR19973062.1610654/150‑1 (MQ=60)
                           CTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGC                                                                                                                           <  SRR19973062.5257993/150‑1 (MQ=60)
                            TGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCA                                                                                                                          >  SRR19973062.4766994/1‑150 (MQ=60)
                            TGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCA                                                                                                                          >  SRR19973062.795827/1‑150 (MQ=60)
                            TGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCA                                                                                                                          <  SRR19973062.4583120/150‑1 (MQ=60)
                             GATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCAC                                                                                                                         >  SRR19973062.808814/1‑150 (MQ=60)
                               TTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCC                                                                                                                       >  SRR19973062.3448728/1‑150 (MQ=60)
                               TTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCC                                                                                                                       >  SRR19973062.103610/1‑150 (MQ=60)
                                TAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCA                                                                                                                      <  SRR19973062.4128241/150‑1 (MQ=60)
                                TAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCA                                                                                                                      <  SRR19973062.4128237/150‑1 (MQ=60)
                                  GAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACA                                                                                                                    >  SRR19973062.1813410/1‑150 (MQ=60)
                                  GAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACA                                                                                                                    >  SRR19973062.3126190/1‑150 (MQ=60)
                                   AAAATAAATTTTTCTTACGCAGACGGGTGTGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAG                                                                                                                   <  SRR19973062.4359529/150‑1 (MQ=60)
                                   AAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAG                                                                                                                   >  SRR19973062.3599586/1‑150 (MQ=60)
                                   AAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAG                                                                                                                   <  SRR19973062.4528327/150‑1 (MQ=60)
                                     AATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAA                                                                                                                 >  SRR19973062.1253788/1‑150 (MQ=60)
                                     AATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAA                                                                                                                 >  SRR19973062.5142483/1‑150 (MQ=60)
                                      ATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAA                                                                                                                >  SRR19973062.3014035/1‑150 (MQ=60)
                                      ATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAA                                                                                                                >  SRR19973062.1662120/1‑150 (MQ=60)
                                       TAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAAT                                                                                                               <  SRR19973062.4875919/150‑1 (MQ=60)
                                       TAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGAGCCAGCACGGCACCCACAGAAAT                                                                                                               <  SRR19973062.2933201/150‑1 (MQ=60)
                                         AATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAA                                                                                                             <  SRR19973062.3962410/150‑1 (MQ=60)
                                          ATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGACATAAA                                                                                                            >  SRR19973062.4091418/1‑150 (MQ=60)
                                          ATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAA                                                                                                            >  SRR19973062.102802/1‑150 (MQ=60)
                                          ATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAA                                                                                                            >  SRR19973062.1216662/1‑150 (MQ=60)
                                          ATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAA                                                                                                            >  SRR19973062.1749905/1‑150 (MQ=60)
                                          ATTTTTCTTAAGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAA                                                                                                            >  SRR19973062.102750/1‑150 (MQ=60)
                                           TTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAAC                                                                                                           >  SRR19973062.1482690/1‑150 (MQ=60)
                                            TTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACA                                                                                                          >  SRR19973062.5295748/1‑150 (MQ=60)
                                               TCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGC                                                                                                       <  SRR19973062.4956806/150‑1 (MQ=60)
                                                CTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCC                                                                                                      >  SRR19973062.653644/1‑150 (MQ=60)
                                                CTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCC                                                                                                      >  SRR19973062.4193252/1‑150 (MQ=60)
                                                CTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCC                                                                                                      <  SRR19973062.3920564/150‑1 (MQ=60)
                                                CTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCC                                                                                                      <  SRR19973062.69779/150‑1 (MQ=60)
                                                CTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCC                                                                                                      >  SRR19973062.3534879/1‑150 (MQ=60)
                                                CTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCC                                                                                                      >  SRR19973062.3534658/1‑150 (MQ=60)
                                                CTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCC                                                                                                      >  SRR19973062.653508/1‑150 (MQ=60)
                                                CTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCC                                                                                                      <  SRR19973062.2809689/150‑1 (MQ=60)
                                                 TTACGCAGACGGGTGCGTTTATCCAGCCTCTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCA                                                                                                     <  SRR19973062.2459321/150‑1 (MQ=60)
                                                 TTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCA                                                                                                     <  SRR19973062.52604/150‑1 (MQ=60)
                                                       AGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATA                                                                                               <  SRR19973062.3112284/150‑1 (MQ=60)
                                                       AGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATA                                                                                               >  SRR19973062.1230204/1‑150 (MQ=60)
                                                        GACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCTCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAA                                                                                              >  SRR19973062.1464423/1‑150 (MQ=60)
                                                        GACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAA                                                                                              >  SRR19973062.3889000/1‑150 (MQ=60)
                                                          CGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAA                                                                                            <  SRR19973062.291867/150‑1 (MQ=60)
                                                           GGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAAC                                                                                           >  SRR19973062.3152450/1‑150 (MQ=60)
                                                            GGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACC                                                                                          >  SRR19973062.4193294/1‑150 (MQ=60)
                                                               GCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAG                                                                                       <  SRR19973062.4357404/150‑1 (MQ=60)
                                                                CGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGC                                                                                      >  SRR19973062.1248964/1‑150 (MQ=60)
                                                                 GTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCA                                                                                     <  SRR19973062.4763273/150‑1 (MQ=60)
                                                                 GTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCA                                                                                     >  SRR19973062.2256604/1‑150 (MQ=60)
                                                                   TTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACC                                                                                   <  SRR19973062.2723928/150‑1 (MQ=60)
                                                                     ATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCAC                                                                                 <  SRR19973062.3175378/150‑1 (MQ=60)
                                                                      TCCAGCCTCTCGGTCATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAAAATCGCACGCGCAAGCAAGTCACCAACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACG                                                                                <  SRR19973062.3790608/150‑1 (MQ=60)
                                                                       CCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGT                                                                               <  SRR19973062.1626905/150‑1 (MQ=60)
                                                                       CCAGCCACTCGGACATTGGGATAGAGGCAAAAACGCTGATGCCGAGCTCTTCCAGTTGTACCGGGGCTTCTACACCACTACGCAACAACGCACGCGCCAGCACGGCACCCACAGACAAAAACATGCCACGAATAAAACCAAGCACCACGT                                                                               >  SRR19973062.3055931/1‑150 (MQ=60)
                                                                        CAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTT                                                                              <  SRR19973062.4491844/150‑1 (MQ=60)
                                                                         AGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTC                                                                             >  SRR19973062.288323/1‑150 (MQ=60)
                                                                         AGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTC                                                                             >  SRR19973062.375064/1‑150 (MQ=60)
                                                                           CCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAA                                                                           <  SRR19973062.1510132/150‑1 (MQ=60)
                                                                            CACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAAC                                                                          >  SRR19973062.1312581/1‑150 (MQ=60)
                                                                            CACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAAC                                                                          <  SRR19973062.4602963/150‑1 (MQ=60)
                                                                            CACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAAC                                                                          <  SRR19973062.4357713/150‑1 (MQ=60)
                                                                                 GGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTT                                                                     <  SRR19973062.306896/150‑1 (MQ=60)
                                                                                 GGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTT                                                                     <  SRR19973062.307092/150‑1 (MQ=60)
                                                                                 GGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTT                                                                     <  SRR19973062.2574283/150‑1 (MQ=60)
                                                                                  GACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTT                                                                    <  SRR19973062.2419562/150‑1 (MQ=60)
                                                                                  GACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTT                                                                    <  SRR19973062.1316310/150‑1 (MQ=60)
                                                                                  GACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTT                                                                    >  SRR19973062.2215700/1‑150 (MQ=60)
                                                                                    CATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTT                                                                  >  SRR19973062.4571754/1‑150 (MQ=60)
                                                                                     ATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTC                                                                 >  SRR19973062.4290487/1‑150 (MQ=60)
                                                                                     ATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTC                                                                 >  SRR19973062.3381779/1‑150 (MQ=60)
                                                                                     ATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCGTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCTCGTTCAACGCTTTTTTC                                                                 <  SRR19973062.3249359/150‑1 (MQ=60)
                                                                                       TGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGG                                                               <  SRR19973062.4609271/150‑1 (MQ=60)
                                                                                        GGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGT                                                              >  SRR19973062.401108/1‑150 (MQ=60)
                                                                                           ATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTC                                                           <  SRR19973062.5140199/150‑1 (MQ=60)
                                                                                             AGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCAC                                                         <  SRR19973062.3543972/150‑1 (MQ=60)
                                                                                             AGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCAC                                                         <  SRR19973062.5006336/150‑1 (MQ=60)
                                                                                                GGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGG                                                      <  SRR19973062.2641165/150‑1 (MQ=60)
                                                                                                   ATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTG                                                   >  SRR19973062.541175/1‑150 (MQ=60)
                                                                                                    TAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGC                                                  <  SRR19973062.689386/150‑1 (MQ=60)
                                                                                                     AAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCG                                                 <  SRR19973062.1600376/150‑1 (MQ=60)
                                                                                                       ACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGC                                               <  SRR19973062.259019/150‑1 (MQ=60)
                                                                                                         GCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTG                                             >  SRR19973062.4814933/1‑150 (MQ=60)
                                                                                                           TGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAG                                           >  SRR19973062.2510903/1‑150 (MQ=60)
                                                                                                           TGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAG                                           >  SRR19973062.1644849/1‑150 (MQ=60)
                                                                                                            GATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGT                                          <  SRR19973062.3230206/150‑1 (MQ=60)
                                                                                                            GATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGT                                          <  SRR19973062.3271010/150‑1 (MQ=60)
                                                                                                                CCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACT                                      <  SRR19973062.4838976/150‑1 (MQ=60)
                                                                                                                 CGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTG                                     >  SRR19973062.5021907/1‑150 (MQ=60)
                                                                                                                      TCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGG                                >  SRR19973062.2848378/1‑150 (MQ=60)
                                                                                                                      TCTTCCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGG                                >  SRR19973062.2848501/1‑150 (MQ=60)
                                                                                                                          CCAGTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGA                            >  SRR19973062.1436160/1‑150 (MQ=60)
                                                                                                                             GTTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAA                         >  SRR19973062.1410716/1‑150 (MQ=60)
                                                                                                                              TTGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAAT                        <  SRR19973062.2806025/150‑1 (MQ=60)
                                                                                                                               TGTTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATC                       <  SRR19973062.2174290/150‑1 (MQ=60)
                                                                                                                                 TTCCGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCG                     <  SRR19973062.977480/150‑1 (MQ=60)
                                                                                                                                    CGGGGCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCAC                  <  SRR19973062.1763266/150‑1 (MQ=60)
                                                                                                                                       GGCTTCTACCCCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTTCCGGGTCGATAATCCGCACGTT               <  SRR19973062.661171/150‑1 (MQ=60)
                                                                                                                                        GCTTCTACACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTA              <  SRR19973062.3318609/150‑1 (MQ=60)
                                                                                                                                               CACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGG       <  SRR19973062.198087/150‑1 (MQ=60)
                                                                                                                                               CACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGG       <  SRR19973062.4370340/150‑1 (MQ=60)
                                                                                                                                                ACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGC      >  SRR19973062.2093761/1‑150 (MQ=60)
                                                                                                                                                ACCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGC      >  SRR19973062.2142813/1‑150 (MQ=60)
                                                                                                                                                 CCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCA     <  SRR19973062.1253107/150‑1 (MQ=60)
                                                                                                                                                 CCACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCA     <  SRR19973062.4191423/150‑1 (MQ=60)
                                                                                                                                                  CACTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCAC    <  SRR19973062.2867335/150‑1 (MQ=60)
                                                                                                                                                    CTACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCACTG  >  SRR19973062.4684640/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GGGGATATTTTTAGTACGATGGCGCTGCTGATTAGAAAATAAATTTTTCTTACGCAGACGGGTGCGTTTATCCAGCCACTCGGACATTGGGATAGTGGCATAAACGCTGATGCCGTGCTCTTCCAGTTGTTCCGGGGCTTCTACACCACGACGCAACATCGCACGCGCCAGCACGGCACCCACAGAAATAAACAGGCCAAGAATAAAACCAAGCACCACGTTCAACGCTTTTTTCGGTTTCACTGGCTGCGGCTGAGTGACTGCCGGGTCGATAATCCGCACGTTACCAATGGCACTG  >  CP009273/1038167‑1038464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑