Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,147,364 G→T 6.4% intergenic (+57/‑31) acpP → / → fabF acyl carrier protein (ACP)/3‑oxoacyl‑[acyl‑carrier‑protein] synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,147,3640GT 6.4% 745.4 / 17.8 203intergenic (+57/‑31)acpP/fabFacyl carrier protein (ACP)/3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
Reads supporting (aligned to +/- strand):  ref base G (101/89);  new base T (2/11);  total (103/100)
Fisher's exact test for biased strand distribution p-value = 9.49e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

Only 100 of 203 total aligned reads displayed.

GAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGA  >  CP009273/1147218‑1147510
                                                                                                                                                  |                                                                                                                                                  
gagTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGa                                                                                                                                                   >  2:444159/1‑148 (MQ=255)
gagTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGa                                                                                                                                                   <  2:3396622/148‑1 (MQ=255)
gagTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCGGAGTTTTATCTTTTTGTCCCACTAGa                                                                                                                                                   >  2:459034/1‑148 (MQ=255)
   tttGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATc                                                                                                                                                >  2:3098072/1‑148 (MQ=255)
   tttGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATc                                                                                                                                                >  2:1327400/1‑148 (MQ=255)
    ttGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCa                                                                                                                                               <  2:1226470/148‑1 (MQ=255)
     tGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTGATCTTTTTGTCCCACTAGAATCAt                                                                                                                                              <  1:254822/148‑1 (MQ=255)
         aCTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCGGAGGGTTATCTTTTTGTCCCACTAGAATCAttttt                                                                                                                                          >  2:3043157/1‑148 (MQ=255)
          cTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCAtttttt                                                                                                                                         <  2:2382222/148‑1 (MQ=255)
          cTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTGATCTTTTTGTCCCACTAGAATCAtttttt                                                                                                                                         <  1:1459217/148‑1 (MQ=255)
          cTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGGGATCTTTTTGTCCCACTAGAATCAtttttt                                                                                                                                         <  1:1709516/148‑1 (MQ=255)
           tGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtc                                                                                                                                        >  1:1779378/1‑148 (MQ=255)
            gagaTTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGGTATCTTTTTGTCCCACTAGAATCATTTTTtcc                                                                                                                                       <  1:1587299/148‑1 (MQ=255)
             agaTTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtccc                                                                                                                                      <  2:3511810/148‑1 (MQ=255)
               aTTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtccctc                                                                                                                                    >  2:2625299/1‑148 (MQ=255)
               aTTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtccctc                                                                                                                                    >  2:2563477/1‑148 (MQ=255)
                ttCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTtccctcc                                                                                                                                   <  2:2768321/148‑1 (MQ=255)
                ttCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtccctcc                                                                                                                                   >  2:3109011/1‑148 (MQ=255)
                 tCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtccctccc                                                                                                                                  <  1:1689752/148‑1 (MQ=255)
                 tCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTtccctccc                                                                                                                                  <  1:1879875/148‑1 (MQ=255)
                 tCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTGGATCTTTTTGTCCCACTAGAATCATTTTTtccctccc                                                                                                                                  <  1:913649/148‑1 (MQ=255)
                  ccGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTccctccct                                                                                                                                 <  2:2053602/148‑1 (MQ=255)
                     gACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTgga                                                                                                                              >  1:141728/1‑148 (MQ=255)
                       cGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTGGACCGCCTGAGTGTTATCTTTTTGTCCCCCTAGAATCATTTTTTACCTCCCGggagg                                                                                                                            >  1:313580/1‑148 (MQ=255)
                       cGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTggagg                                                                                                                            <  2:3169960/148‑1 (MQ=255)
                           gaagCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACaa                                                                                                                        >  1:2365135/1‑148 (MQ=255)
                           gaagCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCGGAGGTTTATCTTTTTGTCCCACGAGAATCATTTTTTCCCTCCCTGGAGGACaa                                                                                                                        >  1:88610/1‑148 (MQ=255)
                            aagCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCTTTCGACCGCCTGTGTTTTATGTTTTTGTCCCACTAGAATAATTTTTTCCCTACCTGGAGGACaaa                                                                                                                       >  1:3160568/1‑148 (MQ=255)
                            aagCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACaaa                                                                                                                       >  1:275414/1‑148 (MQ=255)
                               cTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACgt                                                                                                                    >  2:2561531/1‑148 (MQ=255)
                                 gagaAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACgtgt                                                                                                                  <  2:3077619/148‑1 (MQ=255)
                                 gagaAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACgtgt                                                                                                                  >  2:1911379/1‑148 (MQ=255)
                                  agaAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTc                                                                                                                 >  2:2298577/1‑148 (MQ=255)
                                      aaTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAg                                                                                                             <  2:2103101/148‑1 (MQ=255)
                                      aaTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAg                                                                                                             <  2:1239298/148‑1 (MQ=255)
                                        tCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACGAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGcg                                                                                                           >  1:1395551/1‑148 (MQ=255)
                                           ccaccGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGGGGACAAACGTGTCTCAgcggcg                                                                                                        >  1:1601986/1‑148 (MQ=255)
                                                  tCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGtt                                                                                                 >  2:1121989/1‑148 (MQ=255)
                                                     ggCTGCCATTGATTACATCAACGTCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGTGTGTTATCTTTTTTTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTtgtg                                                                                              >  1:2893632/1‑148 (MQ=255)
                                                     ggCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTtgtg                                                                                              <  1:1327400/148‑1 (MQ=255)
                                                      gCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGa                                                                                             <  2:2741864/148‑1 (MQ=255)
                                                         gCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGGGTAGTTGTGACCg                                                                                          >  1:1117096/1‑148 (MQ=255)
                                                          ccATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTAGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATAATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGTCCgg                                                                                         >  1:3318160/1‑148 (MQ=255)
                                                             ttGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGGTTTATATTTTTGTCCCACTAGAATCATTTTTTACCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGGAGTTGTGACCGGACt                                                                                      >  1:1334062/1‑148 (MQ=255)
                                                                  tACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGTCCGGACTGGGCa                                                                                 >  1:2826194/1‑148 (MQ=255)
                                                                  tACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCa                                                                                 >  1:2966447/1‑148 (MQ=255)
                                                                  tACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCa                                                                                 >  2:1750739/1‑148 (MQ=255)
                                                                     aTCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgt                                                                              <  1:529553/148‑1 (MQ=255)
                                                                     aTCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgt                                                                              <  2:141728/148‑1 (MQ=255)
                                                                      tCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGACGTTCGAACGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgtt                                                                             >  2:1697805/1‑148 (MQ=255)
                                                                       cAACGGCCACCAGGCGTAAGTGAACATCTCCCGGCGGTCGTTCGACCGCCTGAGTTTGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgttg                                                                            <  1:1346243/148‑1 (MQ=255)
                                                                        aaCGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtgttgt                                                                           <  1:2561749/148‑1 (MQ=255)
                                                                         aCGGCCACCAGGCGTAAGTGCACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtc                                                                          <  2:1057501/148‑1 (MQ=255)
                                                                         aCGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtc                                                                          <  2:1979943/148‑1 (MQ=255)
                                                                          cGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtct                                                                         <  2:1008448/148‑1 (MQ=255)
                                                                           ggCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtctc                                                                        <  2:1521066/148‑1 (MQ=255)
                                                                            gCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTcc                                                                       <  1:1980733/148‑1 (MQ=255)
                                                                            gCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTcc                                                                       >  2:3539398/1‑148 (MQ=255)
                                                                             ccaccaGGCGTAAGTGAACATCTCCAGGCGGTCGTTGGGCCGCCGGAGTTTTATCTTTTTTTCCCCCTAGAATCATTTTTTCCCGCCCTGGCGGACAAACGTGTCTAAGCGGCGGGTGGTTGTGTCCGGGCTGGGGAGGTTGTCTCCt                                                                      >  1:2107318/1‑148 (MQ=255)
                                                                                 caGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCg                                                                  >  2:433843/1‑148 (MQ=255)
                                                                                    gCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCa                                                               <  1:780053/148‑1 (MQ=255)
                                                                                    gCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCa                                                               >  2:3501701/1‑148 (MQ=255)
                                                                                           tGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATGTTTTTTTCCCACTAGAATCATTTTTTACCTCCCTGGAGGACAAAAGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTATCCTGTCGGCAATACCGt                                                        >  1:3419170/1‑148 (MQ=255)
                                                                                           tGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGt                                                        >  2:532735/1‑148 (MQ=255)
                                                                                           tGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGt                                                        <  2:1077796/148‑1 (MQ=255)
                                                                                                aTCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGt                                                   <  2:803603/148‑1 (MQ=255)
                                                                                                 tctcCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTc                                                  <  1:3508682/148‑1 (MQ=255)
                                                                                                 tctcCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTc                                                  <  2:946116/148‑1 (MQ=255)
                                                                                                 tctcCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTc                                                  <  1:3109011/148‑1 (MQ=255)
                                                                                                 tctcCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTc                                                  <  1:1061261/148‑1 (MQ=255)
                                                                                                    ccAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTAc                                               <  1:413421/148‑1 (MQ=255)
                                                                                                     cAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTAcc                                              <  2:1691006/148‑1 (MQ=255)
                                                                                                        gCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTgg                                           >  2:3398650/1‑148 (MQ=255)
                                                                                                          ggTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGaa                                         <  2:3139511/148‑1 (MQ=255)
                                                                                                          ggTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGaa                                         <  1:3077590/148‑1 (MQ=255)
                                                                                                            tCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCAGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTTTCTACTGTCGGCAAAACCGTAGAGTCTACCTGGAAAg                                       >  2:298715/1‑148 (MQ=255)
                                                                                                                tCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGctct                                   <  2:3232887/148‑1 (MQ=255)
                                                                                                                  gACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGc                                 <  2:1399928/148‑1 (MQ=255)
                                                                                                                  gACCGCCTGAGTGTGATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGc                                 <  1:2088501/148‑1 (MQ=255)
                                                                                                                   aCCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCt                                >  2:3286336/1‑148 (MQ=255)
                                                                                                                      gCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGc                             <  1:1187874/148‑1 (MQ=255)
                                                                                                                       ccTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGcc                            >  1:2131682/1‑148 (MQ=255)
                                                                                                                        cTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCg                           >  1:2410827/1‑148 (MQ=255)
                                                                                                                           aGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTc                        >  1:1480240/1‑148 (MQ=255)
                                                                                                                              tttATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCaga                     >  2:3187063/1‑148 (MQ=255)
                                                                                                                              tttATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCaga                     <  2:1395551/148‑1 (MQ=255)
                                                                                                                                  tCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTgg                 >  2:1880340/1‑148 (MQ=255)
                                                                                                                                     ttttGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCAt              >  2:662737/1‑148 (MQ=255)
                                                                                                                                     ttttGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCAt              <  1:3050440/148‑1 (MQ=255)
                                                                                                                                      tttGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATc             <  2:2893632/148‑1 (MQ=255)
                                                                                                                                        tGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCCTGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAg           >  2:2432698/1‑148 (MQ=255)
                                                                                                                                        tGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAg           >  2:975159/1‑148 (MQ=255)
                                                                                                                                         gTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGc          <  1:549532/148‑1 (MQ=255)
                                                                                                                                          tCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGcc         <  1:353772/148‑1 (MQ=255)
                                                                                                                                          tCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGcc         >  1:3112164/1‑148 (MQ=255)
                                                                                                                                           cccACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGTCTGGGCATGTTGTCTCCTGTCGGCTATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCt        >  2:879116/1‑148 (MQ=255)
                                                                                                                                              aCTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAAt     >  2:1356188/1‑148 (MQ=255)
                                                                                                                                                tAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCg   >  2:2523660/1‑148 (MQ=255)
                                                                                                                                                tAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCg   >  1:3407445/1‑148 (MQ=255)
                                                                                                                                                tAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCg   >  1:2124116/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGA  >  CP009273/1147218‑1147510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A