Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,147,364 G→T 100% intergenic (+57/‑31) acpP → / → fabF acyl carrier protein (ACP)/3‑oxoacyl‑[acyl‑carrier‑protein] synthase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,147,3640GT97.4% 667.0 / 7.3 227intergenic (+57/‑31)acpP/fabFacyl carrier protein (ACP)/3‑oxoacyl‑[acyl‑carrier‑protein] synthase II
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base T (123/96);  total (128/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 227 total aligned reads displayed.

GTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAAT  >  CP009273/1147220‑1147507
                                                                                                                                                |                                                                                                                                               
gTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAAt                                                                                                                                              >  2:568353/1‑148 (MQ=255)
 tttGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTCTAATc                                                                                                                                             >  2:1965748/1‑148 (MQ=255)
 tttGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATc                                                                                                                                             >  2:1301102/1‑148 (MQ=255)
  ttGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCa                                                                                                                                            >  2:739813/1‑148 (MQ=255)
  ttGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTGTTATCTTTTTGTCCCACGATAATCa                                                                                                                                            >  2:3448026/1‑148 (MQ=255)
   tGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCAt                                                                                                                                           <  2:90580/148‑1 (MQ=255)
    gATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCAtt                                                                                                                                          <  2:2501584/148‑1 (MQ=255)
    gATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACAGCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCAtt                                                                                                                                          <  2:409113/148‑1 (MQ=255)
      tACTGAGATTCCGGACGAACAAGCTGAGAAAATCACCACAGATCAGGCTGCCATTGATTACATAAAACGCCACCAGCCGTAATTGAACATCTCCAGGCGGACGTTCGACCGCCATAGTTTTATCTTTTTGTACCACAAAAATCAtttt                                                                                                                                        >  2:2080717/1‑148 (MQ=255)
       aCTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCAttttt                                                                                                                                       <  1:2599839/148‑1 (MQ=255)
       aCTGAGAGTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTGGTCCCACTATAATCAttttt                                                                                                                                       <  2:531640/148‑1 (MQ=255)
        cTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGGTTTATCTTTTTGTCCCACTATAATCAtttttt                                                                                                                                      >  1:980538/1‑148 (MQ=255)
         tGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCAtttttgc                                                                                                                                     <  2:331616/148‑3 (MQ=255)
         tGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTtc                                                                                                                                     >  2:1022401/1‑148 (MQ=255)
          gagaTTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTtcc                                                                                                                                    <  2:3095625/148‑1 (MQ=255)
           agaTTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTtccc                                                                                                                                   >  2:1828575/1‑148 (MQ=255)
             aTTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTtccctc                                                                                                                                 <  1:2858112/148‑1 (MQ=255)
                 cGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTg                                                                                                                             <  1:798895/148‑1 (MQ=255)
                    aCGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTggag                                                                                                                          >  2:1543819/1‑148 (MQ=255)
                     cgcagaagCTGAGAAAATCCCCACCGTTCAGGCTGCCTTTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTggagg                                                                                                                         <  2:516821/145‑1 (MQ=255)
                      gaagaaGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTggagga                                                                                                                        <  1:3379717/148‑1 (MQ=255)
                      gaagaaGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCCCCGGAGTGTTATATTTTTGTCCCACTATTATCATTTTTTCCCTCCCCggtggt                                                                                                                        >  1:2546531/1‑147 (MQ=255)
                        agaagCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACa                                                                                                                      <  2:3375008/148‑1 (MQ=255)
                        agaagCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACa                                                                                                                      <  2:3329670/148‑1 (MQ=255)
                          aagCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACaaa                                                                                                                    >  2:3260099/1‑148 (MQ=255)
                           agCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAAc                                                                                                                   <  1:583400/148‑1 (MQ=255)
                               gagaAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACgtgt                                                                                                               >  2:3124776/1‑148 (MQ=255)
                                 gaAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGTCCGCCGGAGTTTTTTGTTTTTGTGCCACTATAATCATTTTTTGCCTCCCTGGTGGACAAACGTGTCt                                                                                                             >  1:2950202/1‑148 (MQ=255)
                                 gaAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGCCAAACggggct                                                                                                             >  1:102250/1‑143 (MQ=255)
                                 gaAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCt                                                                                                             <  1:220032/148‑1 (MQ=255)
                                   aaaTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATGTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTaa                                                                                                           >  1:1476551/1‑148 (MQ=255)
                                   aaaTCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTaa                                                                                                           <  1:2933456/148‑1 (MQ=255)
                                      tCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGcg                                                                                                        <  1:1431735/148‑1 (MQ=255)
                                        accaccGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGGCTAAGcgtc                                                                                                      >  1:1964710/1‑148 (MQ=255)
                                         ccaccGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGcgtcg                                                                                                     <  2:2605223/148‑1 (MQ=255)
                                         ccaccGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGcgtcg                                                                                                     <  2:145789/148‑1 (MQ=255)
                                          caccGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATGATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGcgtcgt                                                                                                    >  2:698088/1‑148 (MQ=255)
                                          caccGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGcgtcgt                                                                                                    >  2:3223927/1‑148 (MQ=255)
                                            ccGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCGGGAGGACAAACGTGTCTAAGCGTCgtgt                                                                                                  >  1:837282/1‑148 (MQ=255)
                                             cGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCGGAGGTTTATCTTTTTGTCCCACGATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGGCGAAGCGTCGTGTa                                                                                                 >  1:2392381/1‑148 (MQ=255)
                                                     cTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGAc                                                                                         <  2:350858/148‑1 (MQ=255)
                                                       gCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGTCCg                                                                                       >  1:2139512/1‑148 (MQ=255)
                                                        ccATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCgg                                                                                      <  1:2085566/148‑1 (MQ=255)
                                                        ccATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCgg                                                                                      <  2:652956/148‑1 (MQ=255)
                                                        ccATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCgg                                                                                      >  2:1923623/1‑148 (MQ=255)
                                                        ccATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGGAGTTGTGACCgg                                                                                      >  1:2552582/1‑148 (MQ=255)
                                                         cATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGa                                                                                     <  2:2655132/148‑1 (MQ=255)
                                                          aTTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGAc                                                                                    >  2:3155445/1‑148 (MQ=255)
                                                           ttGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACt                                                                                   >  2:2726009/1‑148 (MQ=255)
                                                             gATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTgg                                                                                 <  1:1177383/148‑1 (MQ=255)
                                                              aTTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTggg                                                                                >  2:419848/1‑148 (MQ=255)
                                                               ttACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGGAGTAGTGACCCGACTGGGc                                                                               >  2:1259735/1‑148 (MQ=255)
                                                                tACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCa                                                                              >  2:1039364/1‑148 (MQ=255)
                                                                  cATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtg                                                                            >  2:368418/1‑148 (MQ=255)
                                                                  cATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCAGTTTTTCCCTCCCTGGAGGACCAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCAtg                                                                            >  2:2206673/1‑148 (MQ=255)
                                                                        cGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTGCCACTAAAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGGGTAGTTGTGTCCGGACTGGGCCTGTTGtct                                                                      >  1:1311122/1‑148 (MQ=255)
                                                                         ggCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtctc                                                                     <  2:1337459/148‑1 (MQ=255)
                                                                         ggCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtctc                                                                     <  1:71303/148‑1 (MQ=255)
                                                                         ggCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGtctc                                                                     <  2:780188/148‑1 (MQ=255)
                                                                            caccaGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTg                                                                  >  2:2927528/1‑148 (MQ=255)
                                                                                  gCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGTGTTTTATCTTTTTGTCCCACGATAATCATTTTTTCCCTCCCGGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCa                                                            >  1:2588050/1‑148 (MQ=255)
                                                                                  gCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAATCGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCa                                                            >  2:3403048/1‑148 (MQ=255)
                                                                                  gCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCa                                                            >  1:2620567/1‑148 (MQ=255)
                                                                                  gCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCa                                                            <  1:3196179/148‑1 (MQ=255)
                                                                                      aaGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGGGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATAc                                                        >  2:2961575/1‑148 (MQ=255)
                                                                                      aaGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCGAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATAc                                                        >  2:420781/1‑148 (MQ=255)
                                                                                        gTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACGATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCg                                                      >  1:2797711/1‑148 (MQ=255)
                                                                                         tGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGt                                                     >  2:1416014/1‑148 (MQ=255)
                                                                                            aCATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTaga                                                  <  2:1646175/148‑1 (MQ=255)
                                                                                              aTCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGt                                                <  1:1414819/148‑1 (MQ=255)
                                                                                                ctcCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCt                                              <  2:176033/148‑1 (MQ=255)
                                                                                                  ccAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTAc                                            >  1:272362/1‑148 (MQ=255)
                                                                                                   cAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTAcc                                           <  2:2549402/148‑1 (MQ=255)
                                                                                                     ggCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTg                                         <  2:2090607/148‑1 (MQ=255)
                                                                                                            gTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGct                                  >  2:239992/1‑148 (MQ=255)
                                                                                                             gtCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGctc                                 <  2:3449794/147‑1 (MQ=255)
                                                                                                              tCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGctct                                >  2:194846/1‑148 (MQ=255)
                                                                                                                 aCCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCt                             <  1:420120/148‑1 (MQ=255)
                                                                                                                       tGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCgg                       >  2:1221549/1‑148 (MQ=255)
                                                                                                                       tGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCgg                       <  2:2691770/148‑1 (MQ=255)
                                                                                                                         aGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTc                     <  1:12020/148‑1 (MQ=255)
                                                                                                                         aGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTc                     >  2:1703205/1‑148 (MQ=255)
                                                                                                                           ttttATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCag                   >  1:1557080/1‑148 (MQ=255)
                                                                                                                            tttATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCaga                  >  2:2043614/1‑148 (MQ=255)
                                                                                                                            tttATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCaga                  <  1:1828575/148‑1 (MQ=255)
                                                                                                                            tttATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCaga                  >  1:2000418/1‑148 (MQ=255)
                                                                                                                             ttATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCagag                 >  2:2862461/1‑148 (MQ=255)
                                                                                                                              tATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGt                >  1:1273784/1‑148 (MQ=255)
                                                                                                                              tATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGt                >  1:3129224/1‑148 (MQ=255)
                                                                                                                              tATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGt                <  1:2686790/148‑1 (MQ=255)
                                                                                                                              tATCTTTTTGTCCCACTATAATCATTTTGTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGt                <  1:1613034/148‑1 (MQ=255)
                                                                                                                               aTCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTg               >  2:3034151/1‑148 (MQ=255)
                                                                                                                               aTCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTg               >  2:1835079/1‑148 (MQ=255)
                                                                                                                               aTCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTg               <  2:866397/148‑1 (MQ=255)
                                                                                                                                tCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTgg              >  2:1071874/1‑148 (MQ=255)
                                                                                                                                 cTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGc             >  1:2177353/1‑148 (MQ=255)
                                                                                                                                 cTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGc             >  1:3071999/1‑148 (MQ=255)
                                                                                                                                   ttttGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCAt           >  2:2758684/1‑148 (MQ=255)
                                                                                                                                   ttttGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCAt           >  2:152240/1‑148 (MQ=255)
                                                                                                                                        tCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGcc      >  1:1074396/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAAT  >  CP009273/1147220‑1147507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGAC  >  CP009273/1147217‑1147511
                                                                                                                                                   |                                                                                                                                                   
ttAGTTTGATACTGAGATTCCGGACGAAGAAGCTGTGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAA                                                                                                                                                   <  SRR19973065.1807488/148‑1 (MQ=60)
     TTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATT                                                                                                                                              <  SRR19973065.2420520/150‑1 (MQ=60)
         TACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTT                                                                                                                                          >  SRR19973065.2750513/1‑150 (MQ=60)
         TACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGGTTTATCTTTTTGTCCCACTATAATCATTTTTT                                                                                                                                          >  SRR19973065.987045/1‑150 (MQ=60)
          ACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTC                                                                                                                                         <  SRR19973065.2615986/150‑1 (MQ=60)
                ATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCC                                                                                                                                   <  SRR19973065.1116045/150‑1 (MQ=60)
                ATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCC                                                                                                                                   <  SRR19973065.2875668/150‑1 (MQ=60)
                    CGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGA                                                                                                                               <  SRR19973065.804175/150‑1 (MQ=60)
                       ACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGA                                                                                                                            <  SRR19973065.3102364/150‑1 (MQ=60)
                       ACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGA                                                                                                                            <  SRR19973065.1316177/150‑1 (MQ=60)
                       ACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCCCCGGAGTGTTATATTTTTGTCCCACTATTATCATTTTTTCCCTCCCcggtggt                                                                                                                            >  SRR19973065.2562477/1‑143 (MQ=60)
                         GAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACA                                                                                                                          <  SRR19973065.1461004/150‑1 (MQ=60)
                         GAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACA                                                                                                                          <  SRR19973065.3400381/150‑1 (MQ=60)
                           AGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAA                                                                                                                        >  SRR19973065.280833/1‑150 (MQ=60)
                            GAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAAC                                                                                                                       <  SRR19973065.1947568/150‑1 (MQ=60)
                            GAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAAC                                                                                                                       <  SRR19973065.3307095/150‑1 (MQ=60)
                              AGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGT                                                                                                                     <  SRR19973065.587034/150‑1 (MQ=60)
                                  GAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGTCCGCCGGAGTTTTTTGTTTTTGTGCCACTATAATCATTTTTTGCCTCCCTGGTGGACAAACGTGTCT                                                                                                                 >  SRR19973065.2968167/1‑150 (MQ=60)
                                  GAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGCCAAACGgggct                                                                                                                 >  SRR19973065.103193/1‑145 (MQ=60)
                                  GAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCGGAGGTTTTTCTTTTTGTCGCACTATAATAATTTTTTCCCTCCCTGGAGGACAAAagtggcg                                                                                                                 >  SRR19973065.2968228/1‑143 (MQ=60)
                                  GAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGAACGCCTGTGTTTTATGTTTTTGTGGCACTTTAATCATTTTTTACCAACAAGGAGGACAAACGTGTCT                                                                                                                 >  SRR19973065.100214/1‑150 (MQ=60)
                                    GAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATGTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAA                                                                                                               >  SRR19973065.1485993/1‑150 (MQ=60)
                                    GAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAA                                                                                                               <  SRR19973065.221671/150‑1 (MQ=60)
                                      AAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGC                                                                                                             <  SRR19973065.2951248/150‑1 (MQ=60)
                                         TCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTC                                                                                                          <  SRR19973065.1441044/150‑1 (MQ=60)
                                         TCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTC                                                                                                          >  SRR19973065.1977713/1‑150 (MQ=60)
                                         TCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGGCTAAGCGTC                                                                                                          >  SRR19973065.1977537/1‑150 (MQ=60)
                                             CACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCGGGAGGACAAACGTGTCTAAGCGTCGTGT                                                                                                      >  SRR19973065.842736/1‑150 (MQ=60)
                                              ACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTA                                                                                                     <  SRR19973065.1477771/150‑1 (MQ=60)
                                              ACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCGGAGGTTTATCTTTTTGTCCCACGATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGGCGAAGCGTCGTGTA                                                                                                     >  SRR19973065.2407412/1‑150 (MQ=60)
                                                    CAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGGGTAGTTGTG                                                                                               >  SRR19973065.2213804/1‑150 (MQ=60)
                                                     AGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGA                                                                                              <  SRR19973065.2630011/150‑1 (MQ=60)
                                                     AGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCGGAGTTTTTTGTTTTTGTGCCACGATAATCATTTTTTCCCTCCCGGGAGGGCAAACGTGTCTAAGCGTGGTGGAGTTGTGA                                                                                              >  SRR19973065.349439/1‑150 (MQ=60)
                                                      GGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGAC                                                                                             <  SRR19973065.2371894/150‑1 (MQ=60)
                                                        CTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGTCCG                                                                                           >  SRR19973065.2153272/1‑150 (MQ=60)
                                                         TGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGG                                                                                          >  SRR19973065.2568502/1‑150 (MQ=60)
                                                         TGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGGAGTTGTGACCGG                                                                                          >  SRR19973065.2568557/1‑150 (MQ=60)
                                                           CCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGAC                                                                                        <  SRR19973065.2098937/150‑1 (MQ=60)
                                                                GATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGC                                                                                   <  SRR19973065.210467/150‑1 (MQ=60)
                                                                GATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGC                                                                                   <  SRR19973065.1184860/150‑1 (MQ=60)
                                                                 ATTACATCAACGGCCACCAGTCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCCCCGGAGTTTTATATTTTTTTACCCCTATAATCATTTTTTACCACCCAGGGGGACAAACGTGTGTAAGCGgcgggttggtttgtccgggcgggggc                                                                                  >  SRR19973065.1533155/1‑124 (MQ=60)
                                                                    ACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGT                                                                               >  SRR19973065.248626/1‑150 (MQ=60)
                                                                       TCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGT                                                                            <  SRR19973065.3081347/150‑1 (MQ=60)
                                                                       TCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATAATTTTTTACCTCCCTGGAGGCCAAAAGTGTCTAAGCGTCGTGTAGTTGTGACCGGACGGGGCCTGTTGT                                                                            >  SRR19973065.2728686/1‑150 (MQ=60)
                                                                        CAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTC                                                                           >  SRR19973065.1975159/1‑150 (MQ=60)
                                                                         AACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTGCCACTAAAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGGGTAGTTGTGTCCGGACTGGGCCTGTTGTCT                                                                          >  SRR19973065.1319781/1‑150 (MQ=60)
                                                                            GGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCT                                                                       <  SRR19973065.72047/150‑1 (MQ=60)
                                                                            GGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCT                                                                       <  SRR19973065.2680341/150‑1 (MQ=60)
                                                                                   AGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGTGTTTTATCTTTTTGTCCCACGATAATCATTTTTTCCCTCCCGGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCA                                                                >  SRR19973065.2604163/1‑150 (MQ=60)
                                                                                   AGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCA                                                                >  SRR19973065.2636788/1‑150 (MQ=60)
                                                                                   AGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATATTTTTGTCCCCCTATAATAATTTTTTACCTCCCTGGAGGACAAACGGGTCTAAGCGGCGTGTAGTTGTGACCGGACTGGGCCTGTTGTGTCCTGTCGGCA                                                                >  SRR19973065.1608973/1‑150 (MQ=60)
                                                                                   AGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCAGAGTTTTATATTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGATGACAAAAGTGTCTAAGCGTAGTGTATTTGTGACCGGAATGGGCATGTTGTGTCCTTTAGGCA                                                                >  SRR19973065.2636763/1‑150 (MQ=60)
                                                                                     GCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAAT                                                              <  SRR19973065.3215399/150‑1 (MQ=60)
                                                                                     GCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAAT                                                              <  SRR19973065.3215439/150‑1 (MQ=60)
                                                                                       GTAAGTGAACATCTCCAGGCGGTCGTTCGGCCGCCGGAGTTTTATCTTTTTTTCCCACAATAATCATTTTTTGCCACCCGGGGGGACAAACGTGTCTAAGCGTCGTGTTGTTGTGTCCGGACTGGGCCTGTTGTCTCCGGTCGGggaaac                                                            >  SRR19973065.725323/1‑144 (MQ=60)
                                                                                         AAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACGATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCG                                                          >  SRR19973065.2815044/1‑150 (MQ=60)
                                                                                         AAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATATTTTTGTCCCACTATAATCATTTTTTACCTCCCTGGAGGACAAACGTGTCTAATCGTCGTGTAGTTGTGACCGGGCTGGGCATGTTGTCTCCGGTCGGCAATAACG                                                          >  SRR19973065.813231/1‑150 (MQ=60)
                                                                                                 ATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCT                                                  <  SRR19973065.1424061/150‑1 (MQ=60)
                                                                                                 ATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCT                                                  <  SRR19973065.1425264/150‑1 (MQ=60)
                                                                                                   CTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTAC                                                >  SRR19973065.274198/1‑150 (MQ=60)
                                                                                                   CTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTAC                                                <  SRR19973065.2952344/150‑1 (MQ=60)
                                                                                                                  CGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCT                                 <  SRR19973065.2074906/150‑1 (MQ=60)
                                                                                                                  CGACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCT                                 <  SRR19973065.290814/150‑1 (MQ=60)
                                                                                                                    ACCGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTG                               <  SRR19973065.423050/150‑1 (MQ=60)
                                                                                                                      CGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCC                             >  SRR19973065.1215076/1‑150 (MQ=60)
                                                                                                                      CGCCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCC                             <  SRR19973065.1813378/150‑1 (MQ=60)
                                                                                                                        CCTGAGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGG                           >  SRR19973065.968029/1‑150 (MQ=60)
                                                                                                                            AGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAATCGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAG                       <  SRR19973065.12135/150‑1 (MQ=60)
                                                                                                                            AGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAG                       >  SRR19973065.1567413/1‑150 (MQ=60)
                                                                                                                            AGTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAG                       <  SRR19973065.12197/150‑1 (MQ=60)
                                                                                                                            AGTTTGATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTTGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTATCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAG                       <  SRR19973065.12216/150‑1 (MQ=60)
                                                                                                                             GTTTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGA                      >  SRR19973065.2013368/1‑150 (MQ=60)
                                                                                                                             GTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGA                      >  SRR19973065.585211/1‑150 (MQ=60)
                                                                                                                               TTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGT                    >  SRR19973065.1282183/1‑150 (MQ=60)
                                                                                                                               TTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGT                    <  SRR19973065.1840621/150‑1 (MQ=60)
                                                                                                                               TTTATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGT                    >  SRR19973065.3148268/1‑150 (MQ=60)
                                                                                                                                 TATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGG                  <  SRR19973065.2703466/150‑1 (MQ=60)
                                                                                                                                 TATCTTTTTGTCCCACTATAATCATTTTGTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGG                  <  SRR19973065.1623857/150‑1 (MQ=60)
                                                                                                                                 TATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGG                  <  SRR19973065.2836523/150‑1 (MQ=60)
                                                                                                                                  ATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGC                 >  SRR19973065.2119538/1‑150 (MQ=60)
                                                                                                                                  ATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGC                 >  SRR19973065.2191314/1‑150 (MQ=60)
                                                                                                                                  ATCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGC                 >  SRR19973065.2191316/1‑150 (MQ=60)
                                                                                                                                  ANCTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGC                 >  SRR19973065.3090775/1‑150 (MQ=60)
                                                                                                                                    CTTTTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCAT               <  SRR19973065.3438269/150‑1 (MQ=60)
                                                                                                                                       TTTGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAG            <  SRR19973065.2600142/150‑1 (MQ=60)
                                                                                                                                         TGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCC          >  SRR19973065.1081501/1‑150 (MQ=60)
                                                                                                                                         TGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCC          >  SRR19973065.1081495/1‑150 (MQ=60)
                                                                                                                                         TGTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCC          >  SRR19973065.140663/1‑150 (MQ=60)
                                                                                                                                          GTCCCACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCT         >  SRR19973065.2608549/1‑150 (MQ=60)
                                                                                                                                              CACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATC     <  SRR19973065.692330/150‑1 (MQ=60)
                                                                                                                                               ACTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCG    <  SRR19973065.842000/150‑1 (MQ=60)
                                                                                                                                                CTATAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGA   >  SRR19973065.835766/1‑150 (MQ=60)
                                                                                                                                                 TATAATCATGTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGAC  <  SRR19973065.2392316/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
AGAGTTTGATACTGAGATTCCGGACGAAGAAGCTGAGAAAATCACCACCGTTCAGGCTGCCATTGATTACATCAACGGCCACCAGGCGTAAGTGAACATCTCCAGGCGGTCGTTCGACCGCCTGAGTTTTATCTTTTTGTCCCACTAGAATCATTTTTTCCCTCCCTGGAGGACAAACGTGTCTAAGCGTCGTGTAGTTGTGACCGGACTGGGCATGTTGTCTCCTGTCGGCAATACCGTAGAGTCTACCTGGAAAGCTCTGCTTGCCGGTCAGAGTGGCATCAGCCTAATCGAC  >  CP009273/1147217‑1147511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑