Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 137,071 G→T 100% I214I (ATC→ATA)  gcd ← glucose dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273137,0710GT96.7% 697.3 / 11.9 226I214I (ATC→ATA) gcdglucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (4/4);  new base T (97/121);  total (101/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 226 total aligned reads displayed.

TGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTC  >  CP009273/136928‑137214
                                                                                                                                               |                                                                                                                                               
tGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGAttt                                                                                                                                             >  2:26241/1‑148 (MQ=255)
  cTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCa                                                                                                                                           <  1:1238291/148‑1 (MQ=255)
      aaaGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCg                                                                                                                                       >  2:1009945/1‑148 (MQ=255)
       aaGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCgg                                                                                                                                      <  2:2943694/148‑1 (MQ=255)
          aCTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTAt                                                                                                                                               >  2:3237307/1‑136 (MQ=255)
          aCTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATc                                                                                                                                   <  1:2914933/148‑1 (MQ=255)
           cTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCg                                                                                                                                  <  2:1613176/148‑1 (MQ=255)
           cTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCAACCGGATCg                                                                                                                                  >  1:3011628/1‑148 (MQ=255)
             cGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACCTCATTGGTTATTTCACCCGGATCGtt                                                                                                                                <  2:3431192/148‑1 (MQ=255)
               ttGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCg                                                                                                                              >  2:2722913/1‑148 (MQ=255)
               ttGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCg                                                                                                                              >  1:2209430/1‑148 (MQ=255)
                tGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCgg                                                                                                                             >  2:3360274/1‑148 (MQ=255)
                tGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCgg                                                                                                                             >  2:1216183/1‑148 (MQ=255)
                tGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCgg                                                                                                                             >  2:1243058/1‑148 (MQ=255)
                   ttttCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGgctg                                                                                                                          <  1:397194/148‑1 (MQ=255)
                    tttCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGgctgc                                                                                                                         <  2:11867/148‑1 (MQ=255)
                     ttCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGgctgct                                                                                                                        <  1:1130411/148‑1 (MQ=255)
                           tCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACAt                                                                                                                  >  2:3164251/1‑148 (MQ=255)
                             aGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCg                                                                                                                <  1:868591/148‑1 (MQ=255)
                                 tCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAg                                                                                                            >  2:290509/1‑148 (MQ=255)
                                 tCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAg                                                                                                            >  2:3200638/1‑148 (MQ=255)
                                 tCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAg                                                                                                            >  1:1175502/1‑148 (MQ=255)
                                  cGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGt                                                                                                           >  2:708185/1‑148 (MQ=255)
                                     aaTGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACg                                                                                                        >  1:1235963/1‑148 (MQ=255)
                                       tGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGa                                                                                                      >  1:653960/1‑148 (MQ=255)
                                        gCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGaa                                                                                                     <  1:3212355/148‑1 (MQ=255)
                                        gCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATAGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGaa                                                                                                     >  1:2274319/1‑148 (MQ=255)
                                          cATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAaca                                                                                                   >  1:105303/1‑148 (MQ=255)
                                           aTTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCAGTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAacac                                                                                                  >  1:1908388/1‑148 (MQ=255)
                                            tttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAcc                                                                                                 <  2:2575608/148‑1 (MQ=255)
                                            tttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAcc                                                                                                 >  2:3310191/1‑148 (MQ=255)
                                            tttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAcc                                                                                                 <  2:886387/148‑1 (MQ=255)
                                            tttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAcc                                                                                                 >  1:2166519/1‑148 (MQ=255)
                                            tttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAcc                                                                                                 <  1:1742059/148‑1 (MQ=255)
                                             ttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAccc                                                                                                >  1:2316846/1‑148 (MQ=255)
                                             ttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAccc                                                                                                >  2:2753941/1‑148 (MQ=255)
                                                 ctTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGc                                                                                            <  1:3269673/148‑1 (MQ=255)
                                                    tGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGGGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGcttc                                                                                         >  1:77603/1‑148 (MQ=255)
                                                     gCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGcttct                                                                                        <  2:3540770/148‑1 (MQ=255)
                                                      ccGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGACCACCCAGGcttctt                                                                                       >  2:2501129/1‑148 (MQ=255)
                                                      ccGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGcttctt                                                                                       <  2:233597/148‑1 (MQ=255)
                                                           ggcggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGa                                                                                  <  1:2589526/148‑1 (MQ=255)
                                                              ggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTa                                                                               <  2:692514/148‑1 (MQ=255)
                                                              ggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTa                                                                               <  2:692998/148‑1 (MQ=255)
                                                               gcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTAt                                                                              <  1:2722913/148‑1 (MQ=255)
                                                               gcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACCTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTAt                                                                              >  2:2961481/1‑148 (MQ=255)
                                                                    aaGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACg                                                                         <  2:3236408/148‑1 (MQ=255)
                                                                    aaGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACg                                                                         >  1:3188844/1‑148 (MQ=255)
                                                                      gcgcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGtt                                                                       >  1:3116387/1‑148 (MQ=255)
                                                                       cgcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGGTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTa                                                                      >  2:2796930/1‑148 (MQ=255)
                                                                        gcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTAt                                                                     <  1:1454277/148‑1 (MQ=255)
                                                                        gcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTAt                                                                     <  1:1642493/148‑1 (MQ=255)
                                                                         cAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATc                                                                    <  2:1153213/148‑1 (MQ=255)
                                                                           aaCAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCgg                                                                  <  2:2673906/148‑1 (MQ=255)
                                                                           aaCAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCgg                                                                  >  1:557053/1‑148 (MQ=255)
                                                                             cAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCg                                                                <  1:707895/148‑1 (MQ=255)
                                                                             cAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCg                                                                <  1:2675096/148‑1 (MQ=255)
                                                                             cAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCg                                                                >  1:1548254/1‑148 (MQ=255)
                                                                             cAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCg                                                                <  2:1516507/148‑1 (MQ=255)
                                                                                gcgcTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTa                                                             >  2:3476622/1‑148 (MQ=255)
                                                                                gcgcTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTa                                                             >  1:724253/1‑148 (MQ=255)
                                                                                 cgcTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTaa                                                            <  1:1008017/148‑1 (MQ=255)
                                                                                  gcTGGTGAGCGGTACAGAGGTATAGGGTGTCGCCCACTTTAATCGGCGTCACGTCATTGTTTATTTCACCCGGATCGTTCGGCTGCTTCACTTCGCCAGTACGGAACTCCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAAt                                                           <  1:3105768/148‑1 (MQ=255)
                                                                                  gcTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAAt                                                           <  1:2026159/148‑1 (MQ=255)
                                                                                   cTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAAtt                                                          <  2:1079097/148‑1 (MQ=255)
                                                                                     ggTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTg                                                        <  2:197713/148‑1 (MQ=255)
                                                                                     ggTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTg                                                        <  1:2754945/148‑1 (MQ=255)
                                                                                        gAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGttt                                                     <  2:2542479/148‑1 (MQ=255)
                                                                                         aGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTc                                                    >  1:3267884/1‑148 (MQ=255)
                                                                                          gCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCa                                                   <  1:3487384/148‑1 (MQ=255)
                                                                                           cGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAg                                                  <  1:3147154/148‑1 (MQ=255)
                                                                                           cGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAg                                                  >  1:2951217/1‑148 (MQ=255)
                                                                                             gTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAgcg                                                <  1:3201196/148‑1 (MQ=255)
                                                                                              tACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAgcgg                                               >  1:246847/1‑148 (MQ=255)
                                                                                              tACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAgcgg                                               <  1:3227437/148‑1 (MQ=255)
                                                                                                cacaGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAgcggcg                                             >  2:308630/1‑148 (MQ=255)
                                                                                                  caGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGaa                                           <  2:3407587/148‑1 (MQ=255)
                                                                                                    ggTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGaaaa                                         >  1:3105149/1‑148 (MQ=255)
                                                                                                        aaGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGt                                     <  1:509589/148‑1 (MQ=255)
                                                                                                           ggTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCACACGCGAAAAGCGTTGa                                  >  1:297730/1‑148 (MQ=255)
                                                                                                             tgtCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGAcc                                >  2:596455/1‑148 (MQ=255)
                                                                                                                 gCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTcc                            <  1:2359693/148‑1 (MQ=255)
                                                                                                                       tttAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTAAGCGGCGAAAAGCGTTGACCTTCCTGATTa                      >  1:1935806/1‑148 (MQ=255)
                                                                                                                          aaTCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGa                   >  2:3005569/1‑148 (MQ=255)
                                                                                                                          aaTCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGa                   <  1:131415/148‑1 (MQ=255)
                                                                                                                           aTCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGAc                  >  1:853631/1‑148 (MQ=255)
                                                                                                                            tCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGAcc                 <  1:3302748/148‑1 (MQ=255)
                                                                                                                            tCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGAcc                 >  2:1853436/1‑148 (MQ=255)
                                                                                                                             cGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCa                >  2:2155428/1‑148 (MQ=255)
                                                                                                                              ggCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCAt               <  2:237019/148‑1 (MQ=255)
                                                                                                                              ggCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCAt               <  2:77603/148‑1 (MQ=255)
                                                                                                                               gCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATa              <  2:2159153/148‑1 (MQ=255)
                                                                                                                               gCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATa              <  2:2432736/148‑1 (MQ=255)
                                                                                                                               gCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACAACGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTTATTTTTTTAACAGGCGCAAAGCGGTTTCCTTAAAGATTAAGAcccaa              >  1:1216128/1‑145 (MQ=255)
                                                                                                                                  tCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGATGACCTTACAGATTAAGAACAAagtc           >  2:418715/1‑146 (MQ=255)
                                                                                                                                   cACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggca          <  2:3179814/148‑1 (MQ=255)
                                                                                                                                     cTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggcagg        <  1:1501191/148‑1 (MQ=255)
                                                                                                                                     cTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggcagg        <  1:1501228/148‑1 (MQ=255)
                                                                                                                                      ttCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggcaggc       <  2:1732394/148‑1 (MQ=255)
                                                                                                                                           tGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTc  >  2:1435509/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
TGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTC  >  CP009273/136928‑137214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGA  >  CP009273/136922‑137218
                                                                                                                                                     |                                                                                                                                                   
GTTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTT                                                                                                                                                     <  SRR19973066.1968270/150‑1 (MQ=60)
 TTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTA                                                                                                                                                    >  SRR19973066.923283/1‑150 (MQ=60)
 TTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTA                                                                                                                                                    <  SRR19973066.1670371/150‑1 (MQ=60)
 TTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTA                                                                                                                                                    <  SRR19973066.1970410/150‑1 (MQ=60)
 TTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTA                                                                                                                                                    <  SRR19973066.2683193/150‑1 (MQ=60)
  TACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTAT                                                                                                                                                   <  SRR19973066.1667335/150‑1 (MQ=60)
    cttccgatctAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTT                                                                                                                                                 <  SRR19973066.3258679/140‑1 (MQ=60)
        CTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACC                                                                                                                                             <  SRR19973066.1245979/150‑1 (MQ=60)
        CTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACC                                                                                                                                             <  SRR19973066.1247118/150‑1 (MQ=60)
         TGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCC                                                                                                                                            >  SRR19973066.2022200/1‑150 (MQ=60)
          GGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCG                                                                                                                                           >  SRR19973066.1429563/1‑150 (MQ=60)
               GACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCAACCGGATCG                                                                                                                                      >  SRR19973066.3031681/1‑150 (MQ=60)
                ACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGT                                                                                                                                     <  SRR19973066.2934380/150‑1 (MQ=60)
                   CGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCG                                                                                                                                  >  SRR19973066.2224731/1‑150 (MQ=60)
                        GTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGC                                                                                                                             >  SRR19973066.3006068/1‑150 (MQ=60)
                         TTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCT                                                                                                                            <  SRR19973066.400322/150‑1 (MQ=60)
                         TTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCT                                                                                                                            <  SRR19973066.401374/150‑1 (MQ=60)
                         TTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCT                                                                                                                            <  SRR19973066.2700236/150‑1 (MQ=60)
                           TTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTC                                                                                                                          <  SRR19973066.2806654/150‑1 (MQ=60)
                           TTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTC                                                                                                                          <  SRR19973066.1138524/150‑1 (MQ=60)
                           TTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTC                                                                                                                          <  SRR19973066.2238665/150‑1 (MQ=60)
                           TTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTC                                                                                                                          <  SRR19973066.387550/150‑1 (MQ=60)
                              AGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACA                                                                                                                       >  SRR19973066.636545/1‑150 (MQ=60)
                                CTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATC                                                                                                                     <  SRR19973066.1516520/150‑1 (MQ=60)
                                   AGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCC                                                                                                                  <  SRR19973066.874661/150‑1 (MQ=60)
                                   AGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCC                                                                                                                  <  SRR19973066.874817/150‑1 (MQ=60)
                                     GATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAG                                                                                                                >  SRR19973066.1183786/1‑150 (MQ=60)
                                        CGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTAC                                                                                                             <  SRR19973066.1660753/150‑1 (MQ=60)
                                         GTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACG                                                                                                            >  SRR19973066.149778/1‑150 (MQ=60)
                                         GTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACG                                                                                                            >  SRR19973066.1244755/1‑150 (MQ=60)
                                           AATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGA                                                                                                          >  SRR19973066.658730/1‑150 (MQ=60)
                                            ATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATAGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAA                                                                                                         >  SRR19973066.2289945/1‑150 (MQ=60)
                                              GCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACA                                                                                                       <  SRR19973066.3233638/150‑1 (MQ=60)
                                              GCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACA                                                                                                       >  SRR19973066.106401/1‑150 (MQ=60)
                                               CCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAC                                                                                                      >  SRR19973066.3110188/1‑150 (MQ=60)
                                               CCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAC                                                                                                      >  SRR19973066.1921996/1‑150 (MQ=60)
                                               CCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAC                                                                                                      >  SRR19973066.3107618/1‑150 (MQ=60)
                                               CCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCAGTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAC                                                                                                      >  SRR19973066.1921985/1‑150 (MQ=60)
                                                CATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACC                                                                                                     >  SRR19973066.2181566/1‑150 (MQ=60)
                                                CATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACC                                                                                                     >  SRR19973066.2296517/1‑150 (MQ=60)
                                                 ATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCC                                                                                                    >  SRR19973066.2332691/1‑150 (MQ=60)
                                                  TTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCA                                                                                                   <  SRR19973066.1754613/150‑1 (MQ=60)
                                                  TTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCA                                                                                                   <  SRR19973066.252957/150‑1 (MQ=60)
                                                     CTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGC                                                                                                >  SRR19973066.1421168/1‑150 (MQ=60)
                                                      TCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCT                                                                                               <  SRR19973066.1227483/150‑1 (MQ=60)
                                                       CTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTT                                                                                              <  SRR19973066.3291140/150‑1 (MQ=60)
                                                       CTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTT                                                                                              <  SRR19973066.2224000/150‑1 (MQ=60)
                                                        TTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGGGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTC                                                                                             >  SRR19973066.78509/1‑150 (MQ=60)
                                                          TGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTT                                                                                           <  SRR19973066.1017389/150‑1 (MQ=60)
                                                          TGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTT                                                                                           >  SRR19973066.3229722/1‑150 (MQ=60)
                                                                TGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGAT                                                                                     >  SRR19973066.3266452/1‑150 (MQ=60)
                                                                TGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGAT                                                                                     >  SRR19973066.881248/1‑150 (MQ=60)
                                                                TGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGAT                                                                                     >  SRR19973066.3168083/1‑150 (MQ=60)
                                                                 GGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATT                                                                                    <  SRR19973066.1183612/150‑1 (MQ=60)
                                                                 GGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATT                                                                                    <  SRR19973066.2607070/150‑1 (MQ=60)
                                                                 GGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATT                                                                                    <  SRR19973066.1184361/150‑1 (MQ=60)
                                                                   CGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTAT                                                                                  <  SRR19973066.2824276/150‑1 (MQ=60)
                                                                    GGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATG                                                                                 <  SRR19973066.3132103/150‑1 (MQ=60)
                                                                     GCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGG                                                                                <  SRR19973066.2741230/150‑1 (MQ=60)
                                                                       ATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGAC                                                                              >  SRR19973066.784888/1‑150 (MQ=60)
                                                                        TCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACG                                                                             >  SRR19973066.3210247/1‑150 (MQ=60)
                                                                        TCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACG                                                                             >  SRR19973066.3210045/1‑150 (MQ=60)
                                                                          AAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTT                                                                           >  SRR19973066.3137204/1‑150 (MQ=60)
                                                                          AAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTT                                                                           >  SRR19973066.1288644/1‑150 (MQ=60)
                                                                            GCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTAT                                                                         <  SRR19973066.1835868/150‑1 (MQ=60)
                                                                             CGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATC                                                                        <  SRR19973066.3311219/150‑1 (MQ=60)
                                                                              GCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCG                                                                       <  SRR19973066.1654443/150‑1 (MQ=60)
                                                                              GCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCG                                                                       <  SRR19973066.3170581/150‑1 (MQ=60)
                                                                              GCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCG                                                                       <  SRR19973066.1464589/150‑1 (MQ=60)
                                                                               CAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGG                                                                      >  SRR19973066.561044/1‑150 (MQ=60)
                                                                                 AACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCG                                                                    >  SRR19973066.1559341/1‑150 (MQ=60)
                                                                                   CAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTT                                                                  <  SRR19973066.713425/150‑1 (MQ=60)
                                                                                   CAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTT                                                                  <  SRR19973066.2692965/150‑1 (MQ=60)
                                                                                   CAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTT                                                                  <  SRR19973066.713134/150‑1 (MQ=60)
                                                                                    AGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTA                                                                 >  SRR19973066.729620/1‑150 (MQ=60)
                                                                                    AGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTGA                                                                 >  SRR19973066.729623/1‑150 (MQ=60)
                                                                                       CGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATT                                                              <  SRR19973066.1015438/150‑1 (MQ=60)
                                                                                        GCTGGTGAGCGGTACAGAGGTATAGGGTGTCGCCCACTTTAATCGGCGTCACGTCATTGTTTATTTCACCCGGATCGTTCGGCTGCTTCACTTCGCCAGTACGGAACTCCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTT                                                             <  SRR19973066.3126506/150‑1 (MQ=60)
                                                                                        GCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTT                                                             <  SRR19973066.2040317/150‑1 (MQ=60)
                                                                                        GCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTT                                                             <  SRR19973066.3126371/150‑1 (MQ=60)
                                                                                           GGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCCCCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGNT                                                          <  SRR19973066.2772485/150‑1 (MQ=60)
                                                                                           GGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTT                                                          <  SRR19973066.2773495/150‑1 (MQ=60)
                                                                                             TGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTC                                                        <  SRR19973066.2661402/150‑1 (MQ=60)
                                                                                             TGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTC                                                        >  SRR19973066.2450090/1‑150 (MQ=60)
                                                                                             TGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTC                                                        >  SRR19973066.3289350/1‑150 (MQ=60)
                                                                                             TGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTC                                                        >  SRR19973066.3289870/1‑150 (MQ=60)
                                                                                              GAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCA                                                       <  SRR19973066.1990038/150‑1 (MQ=60)
                                                                                               AGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAG                                                      >  SRR19973066.2970798/1‑150 (MQ=60)
                                                                                               AGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAG                                                      <  SRR19973066.54240/150‑1 (MQ=60)
                                                                                                GCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGC                                                     <  SRR19973066.697026/150‑1 (MQ=60)
                                                                                                GCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGC                                                     <  SRR19973066.3510649/150‑1 (MQ=60)
                                                                                                GCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGC                                                     <  SRR19973066.3510617/150‑1 (MQ=60)
                                                                                                 CGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCG                                                    <  SRR19973066.3168143/150‑1 (MQ=60)
                                                                                                  GGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGG                                                   <  SRR19973066.2330530/150‑1 (MQ=60)
                                                                                                  GGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGG                                                   >  SRR19973066.248759/1‑150 (MQ=60)
                                                                                                   GTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGC                                                  <  SRR19973066.3222447/150‑1 (MQ=60)
                                                                                                    TACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCG                                                 <  SRR19973066.3248772/150‑1 (MQ=60)
                                                                                                    TACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCG                                                 <  SRR19973066.3248770/150‑1 (MQ=60)
                                                                                                        CAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTTTTTAAGCGGCGAAAA                                             >  SRR19973066.3125885/1‑150 (MQ=60)
                                                                                                        CAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAA                                             >  SRR19973066.2631236/1‑150 (MQ=60)
                                                                                                        CAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTGAATTTTTTTCATCGGCGAAAA                                             >  SRR19973066.832073/1‑150 (MQ=60)
                                                                                                              AAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTG                                       <  SRR19973066.513364/150‑1 (MQ=60)
                                                                                                               AGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCACACGCGAAAAGCGTTGA                                      >  SRR19973066.299838/1‑150 (MQ=60)
                                                                                                                      CGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCT                               <  SRR19973066.1189269/150‑1 (MQ=60)
                                                                                                                       GCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTG                              <  SRR19973066.2375692/150‑1 (MQ=60)
                                                                                                                       GCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTG                              >  SRR19973066.3555478/1‑150 (MQ=60)
                                                                                                                        CCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGA                             >  SRR19973066.1575634/1‑150 (MQ=60)
                                                                                                                        CCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGGTGACCTTCCTGA                             >  SRR19973066.1575680/1‑150 (MQ=60)
                                                                                                                         CCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGAT                            <  SRR19973066.2983091/150‑1 (MQ=60)
                                                                                                                           ACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTAAGCGGCGAAAAGCGTTGACCTTCCTGATTA                          >  SRR19973066.1949542/1‑150 (MQ=60)
                                                                                                                             TTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACG                        <  SRR19973066.704791/150‑1 (MQ=60)
                                                                                                                               TAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGAC                      >  SRR19973066.859799/1‑150 (MQ=60)
                                                                                                                                AATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACC                     <  SRR19973066.132699/150‑1 (MQ=60)
                                                                                                                                  TCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCAT                   <  SRR19973066.3189867/150‑1 (MQ=60)
                                                                                                                                  TCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCAT                   <  SRR19973066.3324592/150‑1 (MQ=60)
                                                                                                                                   CGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACAACGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTtatttttttaacaggcgcaaagcggtttccttaaagattaagacccaa                  >  SRR19973066.1224686/1‑102 (MQ=60)
                                                                                                                                    GGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAG                 <  SRR19973066.2336391/150‑1 (MQ=60)
                                                                                                                                      CGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGC               <  SRR19973066.2956884/150‑1 (MQ=60)
                                                                                                                                        TCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAG             <  SRR19973066.1129937/150‑1 (MQ=60)
                                                                                                                                           CTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCC          <  SRR19973066.1511681/150‑1 (MQ=60)
                                                                                                                                           CTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCC          <  SRR19973066.1511649/150‑1 (MQ=60)
                                                                                                                                           CTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCC          <  SRR19973066.1511686/150‑1 (MQ=60)
                                                                                                                                           CTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTACTGATTACGACCAAAGGCAGGCC          >  SRR19973066.2136442/1‑150 (MQ=60)
                                                                                                                                                   GTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCGTCCTGATTACGACCATAGGCAGGGCAGTCCTGA  >  SRR19973066.1320392/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GTTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTGA  >  CP009273/136922‑137218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑