Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 137,071 G→T 100% I214I (ATC→ATA)  gcd ← glucose dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273137,0710GT98.6% 686.3 / 0.7 211I214I (ATC→ATA) gcdglucose dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (88/120);  total (89/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 211 total aligned reads displayed.

CGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCC  >  CP009273/136926‑137215
                                                                                                                                                 |                                                                                                                                                
cGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTAt                                                                                                                                                >  1:860855/1‑148 (MQ=255)
cGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTAt                                                                                                                                                <  2:2053841/148‑1 (MQ=255)
  tGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTAttt                                                                                                                                              >  1:3138734/1‑148 (MQ=255)
      ggAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCAcc                                                                                                                                          >  2:746219/1‑148 (MQ=255)
       gAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCAccc                                                                                                                                         >  1:3033978/1‑148 (MQ=255)
         aaGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCgg                                                                                                                                       <  1:2761708/148‑1 (MQ=255)
           gACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGAt                                                                                                                                     <  1:712715/148‑1 (MQ=255)
           gACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGAt                                                                                                                                     >  2:809168/1‑148 (MQ=255)
            aCTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATc                                                                                                                                    >  2:862981/1‑148 (MQ=255)
                  tGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCgg                                                                                                                              >  1:2267717/1‑148 (MQ=255)
                     ttttCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGgctg                                                                                                                           <  2:178401/148‑1 (MQ=255)
                       ttCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGgctgct                                                                                                                         <  1:1782547/148‑1 (MQ=255)
                          aGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTca                                                                                                                      <  1:2594423/148‑1 (MQ=255)
                            cTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTcaca                                                                                                                    <  1:2021498/148‑1 (MQ=255)
                            cTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTcaca                                                                                                                    >  1:678726/1‑148 (MQ=255)
                            cTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTcaca                                                                                                                    >  1:1228710/1‑148 (MQ=255)
                            cTCAGGATCGTAATGCCAGTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTcaca                                                                                                                    <  2:2157903/148‑1 (MQ=255)
                             tCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACAt                                                                                                                   <  1:196440/148‑1 (MQ=255)
                             tCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACAt                                                                                                                   <  1:2063147/148‑1 (MQ=255)
                             tCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACAt                                                                                                                   <  1:1397679/148‑1 (MQ=255)
                                ggATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTGCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGc                                                                                                                >  1:15408/1‑148 (MQ=255)
                                 gATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGcc                                                                                                               >  1:432581/1‑148 (MQ=255)
                                 gATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGcc                                                                                                               >  2:2208498/1‑148 (MQ=255)
                                   tCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAg                                                                                                             >  2:3317249/1‑148 (MQ=255)
                                   tCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAg                                                                                                             >  2:85180/1‑148 (MQ=255)
                                     gTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTa                                                                                                           <  2:700350/148‑1 (MQ=255)
                                      tAATGCCATTTCTCTTTGCCGCTGGCGGCATCAATCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTAc                                                                                                          >  1:1655836/1‑148 (MQ=255)
                                      tAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTAc                                                                                                          >  1:3508794/1‑148 (MQ=255)
                                         tGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGa                                                                                                       >  2:3185075/1‑148 (MQ=255)
                                           ccATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAac                                                                                                     <  2:531977/148‑1 (MQ=255)
                                            cATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAaca                                                                                                    <  2:1144035/148‑1 (MQ=255)
                                             aTTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAacac                                                                                                   >  2:385592/1‑148 (MQ=255)
                                              tttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAcc                                                                                                  >  2:1918573/1‑148 (MQ=255)
                                              tttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAcc                                                                                                  <  1:3073231/148‑1 (MQ=255)
                                              tttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAcc                                                                                                  <  1:3073721/148‑1 (MQ=255)
                                              tttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAcc                                                                                                  <  1:1602217/148‑1 (MQ=255)
                                              tttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAcc                                                                                                  >  2:1917663/1‑148 (MQ=255)
                                              ggtctcTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAcc                                                                                                  <  2:2826647/146‑1 (MQ=255)
                                               ttCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACAccc                                                                                                 <  2:2990447/148‑1 (MQ=255)
                                                   ctTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGc                                                                                             <  1:2171792/148‑1 (MQ=255)
                                                     ttGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGctt                                                                                           <  1:1011372/148‑1 (MQ=255)
                                                     ttGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGctt                                                                                           <  1:2695768/148‑1 (MQ=255)
                                                     ttGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGctt                                                                                           <  1:2921039/148‑1 (MQ=255)
                                                      tGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGcttc                                                                                          <  1:2444912/148‑1 (MQ=255)
                                                      tGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGcttc                                                                                          <  1:1198501/148‑1 (MQ=255)
                                                       gCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGcttct                                                                                         <  2:318626/148‑1 (MQ=255)
                                                       gCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGcttct                                                                                         <  1:746219/148‑1 (MQ=255)
                                                       gCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGcttct                                                                                         <  1:54238/148‑1 (MQ=255)
                                                         cGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCttt                                                                                       <  1:1998491/148‑1 (MQ=255)
                                                             ggcggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGa                                                                                   >  1:3270569/1‑148 (MQ=255)
                                                             ggcggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGa                                                                                   <  1:3078279/148‑1 (MQ=255)
                                                                ggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTa                                                                                <  1:2545917/148‑1 (MQ=255)
                                                                ggcATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTa                                                                                <  1:2545876/148‑1 (MQ=255)
                                                                    tCAAGCGCAAACAGGCGCTTGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGa                                                                            <  2:386252/148‑1 (MQ=255)
                                                                    tCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGa                                                                            >  1:2365940/1‑148 (MQ=255)
                                                                      aaGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACg                                                                          <  1:3229846/148‑1 (MQ=255)
                                                                      aaGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACg                                                                          <  1:368806/148‑1 (MQ=255)
                                                                       aGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGt                                                                         >  1:3495510/1‑148 (MQ=255)
                                                                       aGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGt                                                                         <  2:3560839/148‑1 (MQ=255)
                                                                       aGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCGTTCAGATTATGGACGt                                                                         >  1:1067009/1‑148 (MQ=255)
                                                                        gcgcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGtt                                                                        >  2:247507/1‑148 (MQ=255)
                                                                         cgcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTa                                                                       >  2:3033134/1‑148 (MQ=255)
                                                                         cgcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTa                                                                       <  2:2918912/148‑1 (MQ=255)
                                                                          gcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTAt                                                                      <  1:283582/148‑1 (MQ=255)
                                                                          gcAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTAt                                                                      <  2:3065686/148‑1 (MQ=255)
                                                                               cAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCg                                                                 >  2:875438/1‑148 (MQ=255)
                                                                               cAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCg                                                                 <  2:637075/148‑1 (MQ=255)
                                                                                 ggCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGtt                                                               >  1:1432586/1‑148 (MQ=255)
                                                                                  gcgcTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTa                                                              >  1:1110931/1‑148 (MQ=255)
                                                                                        gTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGt                                                        <  1:939895/148‑1 (MQ=255)
                                                                                        gTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGt                                                        <  1:2247238/148‑1 (MQ=255)
                                                                                        gTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGt                                                        <  1:941266/148‑1 (MQ=255)
                                                                                           aGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTc                                                     >  2:360791/1‑148 (MQ=255)
                                                                                             cGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAg                                                   >  1:3294174/1‑148 (MQ=255)
                                                                                                     aGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGaaa                                           >  2:3800760/1‑148 (MQ=255)
                                                                                                      ggTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGaaca                                          >  2:1112501/1‑146 (MQ=255)
                                                                                                       gTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAg                                         <  2:2096290/148‑1 (MQ=255)
                                                                                                             ggTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGa                                   >  2:3597459/1‑148 (MQ=255)
                                                                                                              gtgtCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTAAGCGGCGAAAAGCGTTGAc                                  >  1:1466609/1‑148 (MQ=255)
                                                                                                               tgtCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGAcc                                 <  2:678726/148‑1 (MQ=255)
                                                                                                               tgtCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGAcc                                 <  2:455159/148‑1 (MQ=255)
                                                                                                               tgtCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGAcc                                 <  2:2377685/148‑1 (MQ=255)
                                                                                                                  cGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTc                              <  2:3633528/148‑1 (MQ=255)
                                                                                                                      cACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTTGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGa                          <  2:2725937/148‑1 (MQ=255)
                                                                                                                      cACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGa                          <  2:196063/148‑1 (MQ=255)
                                                                                                                      cACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGa                          <  2:3096616/148‑1 (MQ=255)
                                                                                                                       aCTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGAt                         <  2:720303/148‑1 (MQ=255)
                                                                                                                          ttAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTAAGCGGCGAAAAGCGTTGACCTTCCTGAATAc                      >  1:166000/1‑148 (MQ=255)
                                                                                                                            aaTCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGa                    >  1:3764312/1‑148 (MQ=255)
                                                                                                                             aTCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGAc                   <  1:1926905/148‑1 (MQ=255)
                                                                                                                              tCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTTTTTAAGCGGCGAAAAGCGTTGACCGTCCTGATTACGAcc                  >  1:3267424/1‑148 (MQ=255)
                                                                                                                                    tCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggc            <  1:3053067/148‑1 (MQ=255)
                                                                                                                                     cACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATaggca           >  2:1551793/1‑148 (MQ=255)
                                                                                                                                         tCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGcc       <  2:3138734/148‑1 (MQ=255)
                                                                                                                                         tCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGcc       <  2:1232679/148‑1 (MQ=255)
                                                                                                                                         tCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGcc       <  2:1455136/148‑1 (MQ=255)
                                                                                                                                           aTTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAg     >  2:1609037/1‑148 (MQ=255)
                                                                                                                                             tGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTc   <  1:809168/148‑1 (MQ=255)
                                                                                                                                             tGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTc   <  1:1373575/148‑1 (MQ=255)
                                                                                                                                              ggTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTcc  <  1:143025/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCC  >  CP009273/136926‑137215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTG  >  CP009273/136922‑137217
                                                                                                                                                     |                                                                                                                                                  
GTTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTT                                                                                                                                                    <  SRR19973069.1589159/150‑1 (MQ=60)
GTTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTT                                                                                                                                                    <  SRR19973069.1172390/150‑1 (MQ=60)
GTTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTT                                                                                                                                                    <  SRR19973069.3441006/150‑1 (MQ=60)
GTTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTT                                                                                                                                                    <  SRR19973069.3439740/150‑1 (MQ=60)
  TACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTAT                                                                                                                                                  >  SRR19973069.866871/1‑150 (MQ=60)
    CGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTT                                                                                                                                                >  SRR19973069.1465291/1‑150 (MQ=60)
    CGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTT                                                                                                                                                >  SRR19973069.3159272/1‑150 (MQ=60)
    CGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTT                                                                                                                                                >  SRR19973069.3159292/1‑150 (MQ=60)
    CGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTT                                                                                                                                                >  SRR19973069.1241076/1‑150 (MQ=60)
        CTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACC                                                                                                                                            <  SRR19973069.1692044/150‑1 (MQ=60)
        CTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACC                                                                                                                                            <  SRR19973069.2245941/150‑1 (MQ=60)
         TGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCC                                                                                                                                           >  SRR19973069.3054113/1‑150 (MQ=60)
         TGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCC                                                                                                                                           >  SRR19973069.3054092/1‑150 (MQ=60)
           GAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGG                                                                                                                                         >  SRR19973069.1968285/1‑150 (MQ=60)
             AAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGAT                                                                                                                                       <  SRR19973069.2780141/150‑1 (MQ=60)
               GACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCG                                                                                                                                     >  SRR19973069.309683/1‑150 (MQ=60)
               GACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCG                                                                                                                                     <  SRR19973069.717728/150‑1 (MQ=60)
               GACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCG                                                                                                                                     <  SRR19973069.718237/150‑1 (MQ=60)
                    GTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGG                                                                                                                                >  SRR19973069.2283079/1‑150 (MQ=60)
                           TTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTC                                                                                                                         <  SRR19973069.1795102/150‑1 (MQ=60)
                              AGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACA                                                                                                                      >  SRR19973069.458494/1‑150 (MQ=60)
                              AGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACA                                                                                                                      <  SRR19973069.2611731/150‑1 (MQ=60)
                              AGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACA                                                                                                                      >  SRR19973069.2393807/1‑150 (MQ=60)
                              AGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACA                                                                                                                      >  SRR19973069.683440/1‑150 (MQ=60)
                              AGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACA                                                                                                                      <  SRR19973069.1047311/150‑1 (MQ=60)
                              AGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACA                                                                                                                      >  SRR19973069.1237056/1‑150 (MQ=60)
                              AGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACA                                                                                                                      >  SRR19973069.1237094/1‑150 (MQ=60)
                               GCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACAT                                                                                                                     <  SRR19973069.3258042/150‑1 (MQ=60)
                                CTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATC                                                                                                                    >  SRR19973069.2502728/1‑150 (MQ=60)
                                CTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATC                                                                                                                    <  SRR19973069.2035583/150‑1 (MQ=60)
                                CTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATC                                                                                                                    <  SRR19973069.2035547/150‑1 (MQ=60)
                                 TCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCG                                                                                                                   <  SRR19973069.1407433/150‑1 (MQ=60)
                                 TCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCG                                                                                                                   <  SRR19973069.1407389/150‑1 (MQ=60)
                                 TCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCG                                                                                                                   <  SRR19973069.2077455/150‑1 (MQ=60)
                                 TCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCG                                                                                                                   <  SRR19973069.2077452/150‑1 (MQ=60)
                                 TCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCG                                                                                                                   <  SRR19973069.198027/150‑1 (MQ=60)
                                  CAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTGCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGC                                                                                                                  >  SRR19973069.15630/1‑150 (MQ=60)
                                   AGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCC                                                                                                                 >  SRR19973069.435758/1‑150 (MQ=60)
                                   AGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCC                                                                                                                 >  SRR19973069.3519299/1‑150 (MQ=60)
                                      ATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGT                                                                                                              <  SRR19973069.697642/150‑1 (MQ=60)
                                        CGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAATCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTAC                                                                                                            >  SRR19973069.1667296/1‑150 (MQ=60)
                                        CGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTAC                                                                                                            >  SRR19973069.1667281/1‑150 (MQ=60)
                                        CGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTAC                                                                                                            >  SRR19973069.3531379/1‑150 (MQ=60)
                                             TGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAAC                                                                                                       >  SRR19973069.2621741/1‑150 (MQ=60)
                                                  TTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCA                                                                                                  <  SRR19973069.3093445/150‑1 (MQ=60)
                                                  TTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCA                                                                                                  <  SRR19973069.3093525/150‑1 (MQ=60)
                                                  TTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCA                                                                                                  <  SRR19973069.3094008/150‑1 (MQ=60)
                                                  TTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCA                                                                                                  <  SRR19973069.3094016/150‑1 (MQ=60)
                                                  TTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCA                                                                                                  <  SRR19973069.1613011/150‑1 (MQ=60)
                                                       CTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTT                                                                                             <  SRR19973069.2186622/150‑1 (MQ=60)
                                                         TTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTTGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCT                                                                                           >  SRR19973069.2744196/1‑150 (MQ=60)
                                                         TTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCT                                                                                           <  SRR19973069.2940384/150‑1 (MQ=60)
                                                         TTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCT                                                                                           <  SRR19973069.2713830/150‑1 (MQ=60)
                                                         TTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCT                                                                                           <  SRR19973069.1018344/150‑1 (MQ=60)
                                                          TGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTT                                                                                          <  SRR19973069.2461371/150‑1 (MQ=60)
                                                          TGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTT                                                                                          <  SRR19973069.1206742/150‑1 (MQ=60)
                                                           GCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTT                                                                                         <  SRR19973069.751506/150‑1 (MQ=60)
                                                           GCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTT                                                                                         <  SRR19973069.54911/150‑1 (MQ=60)
                                                             CGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCA                                                                                       <  SRR19973069.2012414/150‑1 (MQ=60)
                                                               CTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGA                                                                                     >  SRR19973069.3291991/1‑150 (MQ=60)
                                                               CTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGA                                                                                     >  SRR19973069.3291986/1‑150 (MQ=60)
                                                               CTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGA                                                                                     >  SRR19973069.3291979/1‑150 (MQ=60)
                                                                TGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGAT                                                                                    >  SRR19973069.2789171/1‑150 (MQ=60)
                                                                 GGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATT                                                                                   <  SRR19973069.3098594/150‑1 (MQ=60)
                                                                 GGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATT                                                                                   <  SRR19973069.3098626/150‑1 (MQ=60)
                                                                    GGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATG                                                                                <  SRR19973069.2562706/150‑1 (MQ=60)
                                                                    GGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATC                                                                                <  SRR19973069.2562728/150‑1 (MQ=60)
                                                                    GGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATC                                                                                <  SRR19973069.2562747/150‑1 (MQ=60)
                                                                      CATCAATCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCACGCTTCTTTCAGATTATGGA                                                                              >  SRR19973069.1661994/1‑150 (MQ=60)
                                                                      CATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGA                                                                              >  SRR19973069.2382008/1‑150 (MQ=60)
                                                                       ATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGAC                                                                             <  SRR19973069.1900245/150‑1 (MQ=60)
                                                                       ATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGAC                                                                             <  SRR19973069.2058777/150‑1 (MQ=60)
                                                                        TCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACG                                                                            <  SRR19973069.200003/150‑1 (MQ=60)
                                                                         CAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGT                                                                           >  SRR19973069.3518034/1‑150 (MQ=60)
                                                                         CAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGT                                                                           >  SRR19973069.1074494/1‑150 (MQ=60)
                                                                         CAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCGTTCAGATTATGGACGT                                                                           >  SRR19973069.1074442/1‑150 (MQ=60)
                                                                          AAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTT                                                                          <  SRR19973069.371506/150‑1 (MQ=60)
                                                                          AAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTT                                                                          <  SRR19973069.371496/150‑1 (MQ=60)
                                                                          AAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTT                                                                          <  SRR19973069.3250968/150‑1 (MQ=60)
                                                                              GCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCG                                                                      <  SRR19973069.285547/150‑1 (MQ=60)
                                                                                   CAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTT                                                                 <  SRR19973069.3697427/150‑1 (MQ=60)
                                                                                   CAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTT                                                                 >  SRR19973069.1442584/1‑150 (MQ=60)
                                                                                   CAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTT                                                                 >  SRR19973069.1442012/1‑150 (MQ=60)
                                                                                    AGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTA                                                                >  SRR19973069.1118681/1‑150 (MQ=60)
                                                                                    AGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTA                                                                >  SRR19973069.3039961/1‑150 (MQ=60)
                                                                                       CGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATT                                                             <  SRR19973069.259096/150‑1 (MQ=60)
                                                                                           GGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTT                                                         <  SRR19973069.2358403/150‑1 (MQ=60)
                                                                                           GGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTT                                                         <  SRR19973069.422711/150‑1 (MQ=60)
                                                                                           GGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTT                                                         <  SRR19973069.2879307/150‑1 (MQ=60)
                                                                                            GTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTT                                                        <  SRR19973069.946246/150‑1 (MQ=60)
                                                                                            GTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTT                                                        <  SRR19973069.946674/150‑1 (MQ=60)
                                                                                            GTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTT                                                        <  SRR19973069.947620/150‑1 (MQ=60)
                                                                                            GTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTT                                                        <  SRR19973069.2262377/150‑1 (MQ=60)
                                                                                             TGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTC                                                       >  SRR19973069.2281823/1‑150 (MQ=60)
                                                                                             TGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTC                                                       <  SRR19973069.676800/150‑1 (MQ=60)
                                                                                               AGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAG                                                     >  SRR19973069.3315744/1‑150 (MQ=60)
                                                                                                          GGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGC                                          <  SRR19973069.159380/150‑1 (MQ=60)
                                                                                                           GTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCG                                         <  SRR19973069.270709/150‑1 (MQ=60)
                                                                                                            TAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTTTTTAAGCGGCGAAAAccct                                        >  SRR19973069.339882/1‑146 (MQ=60)
                                                                                                                GGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGAC                                    >  SRR19973069.1477333/1‑150 (MQ=60)
                                                                                                                GGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGAC                                    >  SRR19973069.1476998/1‑150 (MQ=60)
                                                                                                                GGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTAAGCGGCGAAAAGCGTTGAC                                    >  SRR19973069.1476841/1‑150 (MQ=60)
                                                                                                                      CGCCCACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCT                              <  SRR19973069.3793790/150‑1 (MQ=60)
                                                                                                                          CACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTTTTTAAGCGGCGAAAAGCGTTGACCATACTGATT                          >  SRR19973069.767770/1‑150 (MQ=60)
                                                                                                                          CACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATT                          <  SRR19973069.86083/150‑1 (MQ=60)
                                                                                                                          CACTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATT                          <  SRR19973069.2030887/150‑1 (MQ=60)
                                                                                                                            CTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTAAGCGGCGAAAAGCGTTGACCTTCCTGATTAA                        >  SRR19973069.167298/1‑150 (MQ=60)
                                                                                                                            CTTTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTAAGCGGCGAAAAGCGTTGACCTTCCTGAATAC                        >  SRR19973069.167429/1‑150 (MQ=60)
                                                                                                                              TTAATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTTTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGA                      >  SRR19973069.3788782/1‑150 (MQ=60)
                                                                                                                                AATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTTTTTAAGCGGCGAAAAGCGTTGACCGTCCTGATTACGACC                    >  SRR19973069.3288826/1‑150 (MQ=60)
                                                                                                                                 ATCGGCGTCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCA                   <  SRR19973069.1940143/150‑1 (MQ=60)
                                                                                                                                        TCACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAG            <  SRR19973069.3073272/150‑1 (MQ=60)
                                                                                                                                         CACTTCATTGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGG           <  SRR19973069.1202682/150‑1 (MQ=60)
                                                                                                                                                 TGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCNT   <  SRR19973069.3205774/150‑1 (MQ=60)
                                                                                                                                                 TGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCT   <  SRR19973069.814896/150‑1 (MQ=60)
                                                                                                                                                 TGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCT   <  SRR19973069.921710/150‑1 (MQ=60)
                                                                                                                                                 TGGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCT   <  SRR19973069.1383146/150‑1 (MQ=60)
                                                                                                                                                  GGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTG  <  SRR19973069.144319/150‑1 (MQ=60)
                                                                                                                                                  GGTTATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTG  <  SRR19973069.143378/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
GTTACGTGCTGGAAAGACTCGTTGGTTTTCAGCTCAGGATCGTAATGCCATTTCTCTTTGCCGCTGGCGGCATCAAGCGCAAACAGGCGCTGGTGAGCGGTACACAGGTAAAGGGTGTCGCCCACTTTAATCGGCGTCACTTCATTGGTGATTTCACCCGGATCGTTCGGCTGCTTCACATCGCCAGTACGGAACACCCAGGCTTCTTTCAGATTATGGACGTTATCGGCGTTAATTTGTTTCAGCGGCGAAAAGCGTTGACCTTCCTGATTACGACCATAGGCAGGCCAGTCCTG  >  CP009273/136922‑137217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑