Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 231,378 C→A 100% R26S (CGC→AGC)  yafS → putative S‑adenosyl‑L‑methionine‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273231,3780CA97.6% 773.3 / 6.8 252R26S (CGC→AGC) yafSputative S‑adenosyl‑L‑methionine‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (3/3);  new base A (122/123);  total (125/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 253 total aligned reads displayed.

AATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGC  >  CP009273/231233‑231522
                                                                                                                                                 |                                                                                                                                                
  ttGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCaa                                                                                                                                              <  2:2220594/148‑1 (MQ=255)
  ttGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCaa                                                                                                                                              >  2:507174/1‑148 (MQ=255)
    gTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAgg                                                                                                                                            <  1:460950/148‑1 (MQ=255)
      catcaAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGgcg                                                                                                                                          <  1:394012/148‑1 (MQ=255)
      catcaAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGgcg                                                                                                                                          <  2:1561048/148‑1 (MQ=255)
      catcaAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGgcg                                                                                                                                          <  2:1688550/148‑1 (MQ=255)
      catcaAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGgcg                                                                                                                                          >  1:2230394/1‑148 (MQ=255)
        tcaAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCt                                                                                                                                        >  1:741763/1‑148 (MQ=255)
           aaGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATAGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGa                                                                                                                                     >  2:251823/1‑148 (MQ=255)
            aGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAg                                                                                                                                    >  1:429657/1‑148 (MQ=255)
             ggCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAgc                                                                                                                                   >  2:318723/1‑148 (MQ=255)
             ggCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAgc                                                                                                                                   >  2:1468375/1‑148 (MQ=255)
              gCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAgcg                                                                                                                                  <  1:2385139/148‑1 (MQ=255)
                gggAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGcc                                                                                                                                <  1:1172782/148‑1 (MQ=255)
                  gAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTTATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAg                                                                                                                              >  2:3057381/1‑148 (MQ=255)
                    aTACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCt                                                                                                                            >  1:2251619/1‑148 (MQ=255)
                     tACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTc                                                                                                                           >  1:155983/1‑148 (MQ=255)
                        tGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAAc                                                                                                                        >  1:1005128/1‑148 (MQ=255)
                         gTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGc                                                                                                                             <  1:3521267/142‑1 (MQ=255)
                         gTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGc                                                                                                                             >  2:1758660/1‑142 (MQ=255)
                         gTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGc                                                                                                                             >  2:3521197/1‑142 (MQ=255)
                         gTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGACGCTGGAGCGCCAGCTCAAcc                                                                                                                       <  2:2492410/148‑1 (MQ=255)
                            aaGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGt                                                                                                                    <  1:2910233/148‑1 (MQ=255)
                            aaGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGt                                                                                                                    <  1:1976009/148‑1 (MQ=255)
                             aGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTg                                                                                                                   <  1:2894390/148‑1 (MQ=255)
                             aGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTg                                                                                                                   >  2:606090/1‑148 (MQ=255)
                              gATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTgg                                                                                                                  >  2:2582630/1‑148 (MQ=255)
                              gATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTgg                                                                                                                  <  2:3302432/148‑1 (MQ=255)
                                 taaTAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGAGGCTCCTGATTGCTGGGGCGATTTGCCCTGTGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCACCTCAAACCCAGGTTc                                                                                                               >  1:184563/3‑148 (MQ=255)
                                 tCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTc                                                                                                               >  1:1392543/1‑148 (MQ=255)
                                   aTAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCAc                                                                                                             <  1:2979910/148‑1 (MQ=255)
                                    tAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACt                                                                                                            <  2:1981144/148‑1 (MQ=255)
                                    tAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACt                                                                                                            >  1:1251766/1‑148 (MQ=255)
                                     aGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTa                                                                                                           >  2:517259/1‑148 (MQ=255)
                                       aTTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTaaa                                                                                                         >  2:3415219/1‑148 (MQ=255)
                                       aTTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTaaa                                                                                                         >  1:2567018/1‑148 (MQ=255)
                                        ttACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTaaaa                                                                                                        <  2:117098/148‑1 (MQ=255)
                                          aCCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATg                                                                                                      >  1:1767629/1‑148 (MQ=255)
                                           ccTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGt                                                                                                     >  2:632820/1‑148 (MQ=255)
                                             tctcAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTAt                                                                                                   >  2:2048272/1‑148 (MQ=255)
                                              ctcAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATg                                                                                                  >  2:1037965/1‑148 (MQ=255)
                                               tcAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATgg                                                                                                 <  2:123054/148‑1 (MQ=255)
                                                cAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTgg                                                                                                                  <  1:2151721/130‑1 (MQ=255)
                                                   tgtgAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTAt                                                                                                   <  2:239434/142‑1 (MQ=255)
                                                   tgtgAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGtttt                                                                                             <  2:2835385/148‑1 (MQ=255)
                                                   tgtgAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGtttt                                                                                             >  1:1079829/1‑148 (MQ=255)
                                                   tgtgAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGtttt                                                                                             >  1:645900/1‑148 (MQ=255)
                                                     tgAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCa                                                                                           >  2:3142970/1‑148 (MQ=255)
                                                      gAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCAt                                                                                          <  2:2701884/148‑1 (MQ=255)
                                                      gAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCAt                                                                                          >  2:2306862/1‑148 (MQ=255)
                                                       aaaCGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATc                                                                                         <  1:1173610/148‑1 (MQ=255)
                                                       aaaCGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATc                                                                                         <  1:2776064/148‑1 (MQ=255)
                                                          cGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGc                                                                                      <  2:2855468/148‑1 (MQ=255)
                                                             aaGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTa                                                                                   <  2:1189638/148‑1 (MQ=255)
                                                             aaGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTa                                                                                   >  1:3346127/1‑148 (MQ=255)
                                                               ggTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAg                                                                                 <  1:1640554/148‑1 (MQ=255)
                                                                gTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGa                                                                                >  2:2086715/1‑148 (MQ=255)
                                                                  tgtgATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGAtt                                                                              <  2:1899312/148‑1 (MQ=255)
                                                                   gtgATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTg                                                                             <  2:2572015/148‑1 (MQ=255)
                                                                       tgaAACCGTCAAGAGTCCCTCAAACTGTCTTGGCTCCTGATTGCTTGGGCGATTTGCCCTGTGGAAAGCTTTATAGCAAGGCGCTGGAGAGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCaa                                                                         <  2:751336/148‑1 (MQ=255)
                                                                        gaAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAAt                                                                        <  2:108367/148‑1 (MQ=255)
                                                                           aCCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTa                                                                     <  2:741763/148‑1 (MQ=255)
                                                                           aCCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTa                                                                     <  1:248691/148‑1 (MQ=255)
                                                                           aCCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTa                                                                     <  1:2048221/148‑1 (MQ=255)
                                                                            ccGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTaa                                                                    >  2:1633610/1‑148 (MQ=255)
                                                                               gCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAgcg                                                                 <  2:2591354/148‑1 (MQ=255)
                                                                                  agagTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAg                                                              >  1:3342811/1‑148 (MQ=255)
                                                                                  agagTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAg                                                              >  1:3025874/1‑148 (MQ=255)
                                                                                   gagTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGa                                                             >  2:1371332/1‑148 (MQ=255)
                                                                                    agTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTTCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGaa                                                            >  1:3036076/1‑148 (MQ=255)
                                                                                    agTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGaa                                                            >  1:3035954/1‑148 (MQ=255)
                                                                                     gTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGaaa                                                           <  1:2667501/148‑1 (MQ=255)
                                                                                        ccTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAACTCa                                                        >  2:3564998/1‑148 (MQ=255)
                                                                                        ccTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCa                                                        >  1:1376315/1‑148 (MQ=255)
                                                                                         cTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCaa                                                       <  2:264955/148‑1 (MQ=255)
                                                                                          tCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAAt                                                      <  1:3142970/148‑1 (MQ=255)
                                                                                            aaaCTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTg                                                    <  2:1641033/148‑1 (MQ=255)
                                                                                               cTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGa                                                 >  2:3268822/1‑148 (MQ=255)
                                                                                                tGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGaa                                                >  2:877520/1‑148 (MQ=255)
                                                                                                 gTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAg                                               <  1:318723/148‑1 (MQ=255)
                                                                                                   cGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCg                                             >  2:2962903/1‑148 (MQ=255)
                                                                                                     tGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTg                                           <  2:3413739/148‑1 (MQ=255)
                                                                                                          ccTGATTGCTGGGGCTATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCgg                                      <  1:97452/148‑1 (MQ=255)
                                                                                                            tGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGtt                                    <  2:155690/148‑1 (MQ=255)
                                                                                                             gATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGttt                                   <  1:2856525/148‑1 (MQ=255)
                                                                                                              aTTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTtc                                  <  2:1964198/148‑1 (MQ=255)
                                                                                                              aTTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTtc                                  <  2:2567197/148‑1 (MQ=255)
                                                                                                               ttGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTtct                                 >  1:1443593/1‑148 (MQ=255)
                                                                                                                  cTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCtcat                              <  2:1005128/148‑1 (MQ=255)
                                                                                                                    ggggCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCtcatca                            <  2:3026402/148‑1 (MQ=255)
                                                                                                                     gggCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCaa                           <  1:813202/148‑1 (MQ=255)
                                                                                                                     gggCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCaa                           >  2:363805/1‑148 (MQ=255)
                                                                                                                            ttGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATg                    >  2:3392342/1‑148 (MQ=255)
                                                                                                                            ttGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATg                    <  1:3097677/148‑1 (MQ=255)
                                                                                                                            ttGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATg                    <  1:1622175/148‑1 (MQ=255)
                                                                                                                               cccTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGttt                 <  2:1771885/148‑1 (MQ=255)
                                                                                                                                    gggAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTgcg            >  1:1925204/1‑148 (MQ=255)
                                                                                                                                     ggAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTgcgc           >  1:3493819/1‑148 (MQ=255)
                                                                                                                                      gAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCa          >  2:1333524/1‑148 (MQ=255)
                                                                                                                                          gCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGa      <  2:1443593/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGC  >  CP009273/231233‑231522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCC  >  CP009273/231230‑231524
                                                                                                                                                    |                                                                                                                                                  
TGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAG                                                                                                                                                   >  SRR19973066.2858536/1‑150 (MQ=60)
 GTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGC                                                                                                                                                  >  SRR19973066.109477/1‑150 (MQ=60)
   AATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAA                                                                                                                                                >  SRR19973066.3205124/1‑150 (MQ=60)
       GTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCG                                                                                                                                            <  SRR19973066.464477/150‑1 (MQ=60)
       GTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCG                                                                                                                                            >  SRR19973066.2245796/1‑150 (MQ=60)
        TCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGC                                                                                                                                           >  SRR19973066.1264030/1‑150 (MQ=60)
         CATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCT                                                                                                                                          <  SRR19973066.397117/150‑1 (MQ=60)
         CATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCT                                                                                                                                          <  SRR19973066.2259595/150‑1 (MQ=60)
         CATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCT                                                                                                                                          >  SRR19973066.747272/1‑150 (MQ=60)
          ATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTG                                                                                                                                         <  SRR19973066.3549254/150‑1 (MQ=60)
          ATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTG                                                                                                                                         <  SRR19973066.428595/150‑1 (MQ=60)
             AAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAG                                                                                                                                      <  SRR19973066.3064075/150‑1 (MQ=60)
             AAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAG                                                                                                                                      >  SRR19973066.432978/1‑150 (MQ=60)
                 GCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCC                                                                                                                                  <  SRR19973066.2401215/150‑1 (MQ=60)
                 GCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCC                                                                                                                                  <  SRR19973066.702292/150‑1 (MQ=60)
                   GGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATATCAAGGCGCTGGAGCGCCAG                                                                                                                                >  SRR19973066.2129788/1‑150 (MQ=60)
                   GGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAG                                                                                                                                <  SRR19973066.1181058/150‑1 (MQ=60)
                     GAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCT                                                                                                                              >  SRR19973066.2267144/1‑150 (MQ=60)
                     GAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCT                                                                                                                              <  SRR19973066.2527603/150‑1 (MQ=60)
                      atctCTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTC                                                                                                                             <  SRR19973066.1771291/146‑1 (MQ=60)
                      atctCTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTC                                                                                                                             <  SRR19973066.3544711/146‑1 (MQ=60)
                      atctCTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTC                                                                                                                             <  SRR19973066.3544640/146‑1 (MQ=60)
                      AATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTC                                                                                                                             >  SRR19973066.157131/1‑150 (MQ=60)
                      AATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTC                                                                                                                             >  SRR19973066.157424/1‑150 (MQ=60)
                      AATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTC                                                                                                                             >  SRR19973066.747576/1‑150 (MQ=60)
                        TACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAA                                                                                                                           >  SRR19973066.3084999/1‑150 (MQ=60)
                         ACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAAC                                                                                                                          <  SRR19973066.2917329/150‑1 (MQ=60)
                         ACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAAC                                                                                                                          >  SRR19973066.1012535/1‑150 (MQ=60)
                          CTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACC                                                                                                                         <  SRR19973066.2985474/150‑1 (MQ=60)
                               AAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGG                                                                                                                    <  SRR19973066.1989941/150‑1 (MQ=60)
                               AAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGG                                                                                                                    <  SRR19973066.1989972/150‑1 (MQ=60)
                               AAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGG                                                                                                                    <  SRR19973066.1990390/150‑1 (MQ=60)
                               AAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGG                                                                                                                    <  SRR19973066.2929661/150‑1 (MQ=60)
                                AGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGT                                                                                                                   <  SRR19973066.2913738/150‑1 (MQ=60)
                                 tgtgctcttccgatCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTT                                                                                                                  <  SRR19973066.2166657/136‑1 (MQ=60)
                                  ATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTC                                                                                                                 >  SRR19973066.1402584/1‑150 (MQ=60)
                                  ATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTC                                                                                                                 >  SRR19973066.2987865/1‑150 (MQ=60)
                                  ATTAATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGAGGCTCCTGATTGCTGGGGCGATTTGCCCTGTGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCACCTCAAacccaggttc                                                                                                                 >  SRR19973066.186173/1‑140 (MQ=60)
                                  AGTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTAGAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTC                                                                                                                 <  SRR19973066.3009882/150‑1 (MQ=60)
                                     CATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACT                                                                                                              >  SRR19973066.1260760/1‑150 (MQ=60)
                                     CATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACT                                                                                                              >  SRR19973066.1260732/1‑150 (MQ=60)
                                     CATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACT                                                                                                              >  SRR19973066.3048967/1‑150 (MQ=60)
                                      ATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTA                                                                                                             <  SRR19973066.2999591/150‑1 (MQ=60)
                                        AGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAA                                                                                                           >  SRR19973066.2584437/1‑150 (MQ=60)
                                           TTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATG                                                                                                        >  SRR19973066.1780300/1‑150 (MQ=60)
                                             ACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTA                                                                                                      >  SRR19973066.1784569/1‑150 (MQ=60)
                                               CTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATG                                                                                                    >  SRR19973066.82511/1‑150 (MQ=60)
                                                 CTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGT                                                                                                  >  SRR19973066.1243502/1‑150 (MQ=60)
                                                 ATCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGT                                                                                                  <  SRR19973066.3359694/150‑1 (MQ=60)
                                                   CAGTGTGAACCGGCAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTT                                                                                                <  SRR19973066.2793622/150‑1 (MQ=60)
                                                    AGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGagat                                                                                               >  SRR19973066.241313/1‑146 (MQ=60)
                                                    AGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTT                                                                                               >  SRR19973066.1087718/1‑150 (MQ=60)
                                                    AGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTT                                                                                               >  SRR19973066.451506/1‑150 (MQ=60)
                                                    AGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTT                                                                                               >  SRR19973066.451548/1‑150 (MQ=60)
                                                    AGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTT                                                                                               >  SRR19973066.650581/1‑150 (MQ=60)
                                                     GTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTC                                                                                              <  SRR19973066.358447/150‑1 (MQ=60)
                                                      TGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCA                                                                                             >  SRR19973066.2815669/1‑150 (MQ=60)
                                                       GTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCAT                                                                                            <  SRR19973066.906954/150‑1 (MQ=60)
                                                          AAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAGACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCNGGGGAAAGCGTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTG                                                                                         >  SRR19973066.1820308/1‑150 (MQ=60)
                                                          AAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTG                                                                                         <  SRR19973066.2794751/150‑1 (MQ=60)
                                                          AAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTG                                                                                         <  SRR19973066.1181887/150‑1 (MQ=60)
                                                              GGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTA                                                                                     >  SRR19973066.3368402/1‑150 (MQ=60)
                                                              GGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCGGAGTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAATGAGCTGGAGCGCCAGCTCAACCCGTGGTTCACAAAAATGTAAGGTTTTCATATGCTTA                                                                                     >  SRR19973066.3367206/1‑150 (MQ=60)
                                                                  TGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGAT                                                                                 <  SRR19973066.1652444/150‑1 (MQ=60)
                                                                  GGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGAT                                                                                 <  SRR19973066.1652485/150‑1 (MQ=60)
                                                                              ACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAG                                                                     <  SRR19973066.250610/150‑1 (MQ=60)
                                                                              ACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAG                                                                     <  SRR19973066.2062511/150‑1 (MQ=60)
                                                                              ACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAG                                                                     <  SRR19973066.2062459/150‑1 (MQ=60)
                                                                                   CAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAG                                                                >  SRR19973066.3046275/1‑150 (MQ=60)
                                                                                   CAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAG                                                                >  SRR19973066.3046082/1‑150 (MQ=60)
                                                                                   CAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAG                                                                >  SRR19973066.3365065/1‑150 (MQ=60)
                                                                                     AGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTTCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTTGCAATTTAATCGCAGAA                                                              >  SRR19973066.3056367/1‑150 (MQ=60)
                                                                                     AGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTAATCTGCTTAATATTGGCAATTTAAGCGCAGAA                                                              >  SRR19973066.3056242/1‑150 (MQ=60)
                                                                                     AGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTAATCTGCTTAAGATTGGCAATTTAAGCGCAGAA                                                              >  SRR19973066.3056357/1‑150 (MQ=60)
                                                                                       AGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAAT                                                            <  SRR19973066.3241587/150‑1 (MQ=60)
                                                                                        GTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATC                                                           <  SRR19973066.2685701/150‑1 (MQ=60)
                                                                                        GTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATC                                                           <  SRR19973066.2685346/150‑1 (MQ=60)
                                                                                         TCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCA                                                          >  SRR19973066.1386271/1‑150 (MQ=60)
                                                                                             TCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTG                                                      <  SRR19973066.3163936/150‑1 (MQ=60)
                                                                                               AAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCG                                                    <  SRR19973066.1045602/150‑1 (MQ=60)
                                                                                               AAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCG                                                    >  SRR19973066.3513677/1‑150 (MQ=60)
                                                                                                    GTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCG                                               <  SRR19973066.321113/150‑1 (MQ=60)
                                                                                                          GCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCG                                         <  SRR19973066.983720/150‑1 (MQ=60)
                                                                                                          GCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGAAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCG                                         <  SRR19973066.2268724/150‑1 (MQ=60)
                                                                                                             CCTGATTGCTGGGGCTATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTT                                      <  SRR19973066.98496/150‑1 (MQ=60)
                                                                                                              CTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTT                                     <  SRR19973066.2312614/150‑1 (MQ=60)
                                                                                                                GATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCT                                   >  SRR19973066.1453865/1‑150 (MQ=60)
                                                                                                                GATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCT                                   <  SRR19973066.2875714/150‑1 (MQ=60)
                                                                                                                GATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCT                                   >  SRR19973066.1450676/1‑150 (MQ=60)
                                                                                                                GATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCT                                   <  SRR19973066.2875713/150‑1 (MQ=60)
                                                                                                                 ATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATTGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTC                                  <  SRR19973066.1181516/150‑1 (MQ=60)
                                                                                                                      TGGGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAA                             >  SRR19973066.688280/1‑150 (MQ=60)
                                                                                                                        GGGCGTTGTGCCCTGGGGAAAGCTGTAGAGCAAGGCGCTGTAGCGCCAGCTCAACGCGTGGTTCACTAAAATTTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCTTCAAGT                           <  SRR19973066.3413682/150‑1 (MQ=60)
                                                                                                                        GGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGT                           <  SRR19973066.1478733/150‑1 (MQ=60)
                                                                                                                        GGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGT                           >  SRR19973066.1387720/1‑150 (MQ=60)
                                                                                                                        GGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGT                           <  SRR19973066.819162/150‑1 (MQ=60)
                                                                                                                        GGGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGT                           >  SRR19973066.3173665/1‑150 (MQ=60)
                                                                                                                         GGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTG                          <  SRR19973066.2194328/150‑1 (MQ=60)
                                                                                                                         GGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTG                          <  SRR19973066.2194289/150‑1 (MQ=60)
                                                                                                                         GGCGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTG                          <  SRR19973066.1153013/150‑1 (MQ=60)
                                                                                                                           CGATTTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAA                        >  SRR19973066.1910906/1‑150 (MQ=60)
                                                                                                                               TTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTT                    <  SRR19973066.3118372/150‑1 (MQ=60)
                                                                                                                               TTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTT                    <  SRR19973066.1633931/150‑1 (MQ=60)
                                                                                                                               TTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTT                    <  SRR19973066.1645719/150‑1 (MQ=60)
                                                                                                                               TTGCCCTGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTT                    <  SRR19973066.3118754/150‑1 (MQ=60)
                                                                                                                                     TGGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCG              >  SRR19973066.1938895/1‑150 (MQ=60)
                                                                                                                                      GGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGC             >  SRR19973066.3517118/1‑150 (MQ=60)
                                                                                                                                      GGGGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGC             >  SRR19973066.2641912/1‑150 (MQ=60)
                                                                                                                                        GGAAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAA           >  SRR19973066.1964075/1‑150 (MQ=60)
                                                                                                                                          AAAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGG         <  SRR19973066.2894901/150‑1 (MQ=60)
                                                                                                                                           AAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGA        >  SRR19973066.1612441/1‑150 (MQ=60)
                                                                                                                                           AAGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGA        >  SRR19973066.2548505/1‑150 (MQ=60)
                                                                                                                                            AGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAA       <  SRR19973066.637377/150‑1 (MQ=60)
                                                                                                                                            AGCTTTATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAA       >  SRR19973066.139574/1‑150 (MQ=60)
                                                                                                                                                 TATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCC  >  SRR19973066.3335547/1‑150 (MQ=60)
                                                                                                                                                 TATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCC  >  SRR19973066.3335543/1‑150 (MQ=60)
                                                                                                                                                 TATAGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCC  >  SRR19973066.1115942/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
TGTAATTGTCATCAAAGGCGGGAATACTGTTAAGATTCATAGATTACCTCTCAGTGTGAAACGGAAGGTTGTGATGAAACCGGCAAGAGTCCCTCAAACTGTCGTGGCTCCTGATTGCTGGGGCGATTTGCCCTGGGGAAAGCTTTATCGCAAGGCGCTGGAGCGCCAGCTCAACCCGTGGTTCACTAAAATGTATGGTTTTCATCTGCTTAAGATTGGCAATTTAAGCGCAGAAATCAATTGCGAAGCGTGCGCGGTTTCTCATCAAGTGAATGTTTCTGCGCAAGGAATGCCC  >  CP009273/231230‑231524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑